UBE2M cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	UBE2M
Entrez Gene ID	484221
Full Name	ubiquitin conjugating enzyme E2 M
General protein information	Preferred Names ubiquitin conjugating enzyme E2 M Names NEDD8-conjugating enzyme Ubc12 ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_541337.5	XP_541337.3	NEDD8-conjugating enzyme Ubc12

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	UBE2M	XP_001146573.2
	UBE2M	XP_001099932.1
Bos taurus (cattle)	UBE2M	NP_001076972.1
Schizosaccharomyces pombe (fission yeast)	ubc12	NP_588256.1
Mus musculus (house mouse)	Ube2m	NP_663553.1
Magnaporthe oryzae (rice blast fungus)	MGG_01807	XP_003714818.1
Neurospora crassa	NCU03610	XP_961337.1
Oryza sativa (rice)	Os09g0321900	NP_001062859.1
Homo sapiens (human)	UBE2M	NP_003960.1
Canis lupus familiaris (dog)	UBE2M	XP_541337.3
Drosophila melanogaster (fruit fly)	Ubc12	NP_001261568.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005916	XP_315947.2
Saccharomyces cerevisiae (baker's yeast)	UBC12	NP_013409.1
Arabidopsis thaliana (thale cress)	RCE1	NP_001154289.1
Arabidopsis thaliana (thale cress)	AT2G18600	NP_565440.1
Rattus norvegicus (Norway rat)	Ube2m	NP_001101941.1
Danio rerio (zebrafish)	wu:fb58e05	XP_001920670.3
Kluyveromyces lactis	KLLA0C17248g	XP_452971.1
Oryza sativa (rice)	Os08g0374100	NP_001061664.1
Eremothecium gossypii	AGOS_ADL035C	NP_984062.1
Xenopus tropicalis (tropical clawed frog)	ube2m	NP_988956.1

Gene Ontology

The following UBE2M gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UBE2M cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe01499	XM_541337.5 Latest version!	Canis lupus familiaris ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UBE2M ( XM_541337.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_541337.3 Canis lupus familiaris ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.

Price & Availability↑

CloneID	OCe01499
Clone ID Related Accession (Same CDS sequence)	XM_541337.5
Accession Version	XM_541337.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	NEDD8-conjugating enzyme Ubc12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_541337.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC GGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCTGCG CAGCTGCGGA TCCAGAAGGA CATAAACGAG 
CTAAACCTGC CCAAGACGTG TGACATCAGC TTCTCAGATC CAGACGATCT CCTCAACTTC 
AAGCTGGTTA TCTGTCCTGA TGAGGGCTTC TACAAGAGTG GGAAGTTTGT ATTCAGTTTT 
AAGGTGGGCC AGGGTTACCC ACATGACCCC CCCAAGGTGA AGTGTGAGAC GATGGTCTAT 
CACCCCAACA TTGACCTCGA GGGCAATGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCCAAC 
CCCGAAGACC CACTGAACAA GGAGGCCGCC GAGGTCCTGC AGAACAACCG GCGGCTGTTT 
GAGCAGAATG TGCAGCGCTC CATGCGGGGT GGCTATATCG GCTCCACCTA CTTCGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_541337.3
CDS	239..790
Translation	MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVICPDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIYGLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLK

Target ORF information:

RefSeq Version	XM_541337.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris ubiquitin conjugating enzyme E2 M (UBE2M), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_541337.5

ORF Insert Sequence:

ATGATCAAGC TGTTCTCGCT GAAGCAGCAG AAGAAGGAGG AGGAGTCGGC GGGCGGCACC 
AAGGGCAGCA GCAAGAAGGC GTCGGCTGCG CAGCTGCGGA TCCAGAAGGA CATAAACGAG 
CTAAACCTGC CCAAGACGTG TGACATCAGC TTCTCAGATC CAGACGATCT CCTCAACTTC 
AAGCTGGTTA TCTGTCCTGA TGAGGGCTTC TACAAGAGTG GGAAGTTTGT ATTCAGTTTT 
AAGGTGGGCC AGGGTTACCC ACATGACCCC CCCAAGGTGA AGTGTGAGAC GATGGTCTAT 
CACCCCAACA TTGACCTCGA GGGCAATGTC TGCCTCAACA TCCTCAGAGA GGACTGGAAG 
CCAGTCCTTA CGATAAACTC CATAATTTAT GGCCTGCAGT ATCTCTTCTT GGAGCCCAAC 
CCCGAAGACC CACTGAACAA GGAGGCCGCC GAGGTCCTGC AGAACAACCG GCGGCTGTTT 
GAGCAGAATG TGCAGCGCTC CATGCGGGGT GGCTATATCG GCTCCACCTA CTTCGAGCGC 
TGCCTGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.