DYSF gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_003432223.3	XP_003432271.1	dysferlin isoform X14
XM_022404853.1	XP_022260561.1	dysferlin isoform X10
XM_003432227.3	XP_003432275.1	dysferlin isoform X11
XM_022404847.1	XP_022260555.1	dysferlin isoform X3
XM_003432234.4	XP_003432282.1	dysferlin isoform X9
XM_003432225.4	XP_003432273.1	dysferlin isoform X13
XM_003432226.4	XP_003432274.1	dysferlin isoform X14
XM_003432223.4	XP_003432271.1	dysferlin isoform X24
XM_003432234.3	XP_003432282.1	dysferlin isoform X1
XM_003432230.3	XP_003432278.1	dysferlin isoform X3
XM_003432225.3	XP_003432273.1	dysferlin isoform X4
XM_003432222.3	XP_003432270.1	dysferlin isoform X6
XM_003432233.3	XP_003432281.1	dysferlin isoform X7
XM_003432228.3	XP_003432276.1	dysferlin isoform X8
XM_540237.5	XP_540237.2	dysferlin isoform X12
XM_022404845.1	XP_022260553.1	dysferlin isoform X1
XM_022404846.1	XP_022260554.1	dysferlin isoform X2
XM_022404849.1	XP_022260557.1	dysferlin isoform X5
XM_003432230.4	XP_003432278.1	dysferlin isoform X12
XM_003432228.4	XP_003432276.1	dysferlin isoform X17
XM_003432231.4	XP_003432279.1	dysferlin isoform X18
XM_003432224.4	XP_003432272.1	dysferlin isoform X19
XM_003432227.4	XP_003432275.1	dysferlin isoform X20
XM_540237.6	XP_540237.2	dysferlin isoform X21
XM_003432226.3	XP_003432274.1	dysferlin isoform X5
XM_003432231.3	XP_003432279.1	dysferlin isoform X9
XM_022404852.1	XP_022260560.1	dysferlin isoform X8
XM_003432232.4	XP_003432280.1	dysferlin isoform X23
XM_003432229.3	XP_003432277.1	dysferlin isoform X2
XM_003432224.3	XP_003432272.1	dysferlin isoform X10
XM_003432232.3	XP_003432280.1	dysferlin isoform X13
XM_022404848.1	XP_022260556.1	dysferlin isoform X4
XM_022404850.1	XP_022260558.1	dysferlin isoform X6
XM_022404851.1	XP_022260559.1	dysferlin isoform X7
XM_003432229.4	XP_003432277.1	dysferlin isoform X11
XM_003432222.4	XP_003432270.1	dysferlin isoform X15
XM_003432233.4	XP_003432281.1	dysferlin isoform X16
XM_022404854.1	XP_022260562.1	dysferlin isoform X22

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe294454	XM_022404845.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294455	XM_022404846.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294456	XM_022404847.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294457	XM_022404848.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294458	XM_022404849.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294459	XM_022404850.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294460	XM_022404851.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294461	XM_022404852.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294462	XM_022404853.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe294463	XM_022404854.1 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OCe19206	XM_003432234.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19207	XM_003432229.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19208	XM_003432230.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19209	XM_003432225.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19210	XM_003432226.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19211	XM_003432222.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19212	XM_003432233.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19213	XM_003432228.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19214	XM_003432231.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19215	XM_003432224.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19216	XM_003432227.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19217	XM_540237.6 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19218	XM_003432232.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19219	XM_003432223.4 Latest version!	Canis lupus familiaris dysferlin (DYSF), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OCe19219	XM_003432223.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19206	XM_003432234.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19209	XM_003432225.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19208	XM_003432230.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19213	XM_003432228.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19216	XM_003432227.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19217	XM_540237.5	Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19210	XM_003432226.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19214	XM_003432231.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19215	XM_003432224.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19218	XM_003432232.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19207	XM_003432229.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19211	XM_003432222.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OCe19212	XM_003432233.3	Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

DYSF ( XM_022404845.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260553.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe294454
Clone ID Related Accession (Same CDS sequence)	XM_022404845.1
Accession Version	XM_022404845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT 
GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT 
GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT 
AAGGCCCTCC TGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG 
GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC 
ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG 
GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA 
TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA 
GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA 
CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG 
GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC 
TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC 
CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA 
GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG 
GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG 
TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG 
ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG 
AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC 
GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC 
TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC 
TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC 
TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG 
GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG 
TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG 
GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA 
ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG 
GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC 
TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC 
ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT 
TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260553.1
CDS	23..6487
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEELCAPAFFLQGLLQTSEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHKALLEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404845.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404845.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT 
GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT 
GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT 
AAGGCCCTCC TGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG 
GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC 
ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG 
GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA 
TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA 
GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA 
CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG 
GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC 
TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC 
CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA 
GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG 
GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG 
TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG 
ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG 
AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC 
GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC 
TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC 
TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC 
TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG 
GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG 
TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG 
GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA 
ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG 
GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC 
TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC 
ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT 
TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_022404846.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260554.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	OCe294455
Clone ID Related Accession (Same CDS sequence)	XM_022404846.1
Accession Version	XM_022404846.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC 
CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC 
AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC 
CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT 
GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG 
ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA 
GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA 
GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC 
TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT 
GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT 
CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC 
CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC 
CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC 
AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG 
GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC 
CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT 
GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC 
TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT 
GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT 
GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT 
TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT 
GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG 
CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG 
GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT CCAAACGTCA GAGGACATAG ACCTGCCCTA CCCGCCACCT 
CAGAGGGAGG CCAACATCTT CATGGTCCCC CAGAACATCA AGCCTGTTCT CCAGCGTACT 
GCCATAGAGA TCCTGGCGTG GGGCCTGCGG AACATGAAGA GCTACCAGCT AGCCAGCATC 
TCCTCCCCTA GCCTTGTGGT AGAGTGCGGG GGCCAGACAG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGTACCCTTT TCATGGAAGT GATGCTGCCC 
AAGGAGGAGG TCTACTGTCC CTCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATC CGTTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAATCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT GCAGCTTGCA 
GATGGTCTGT CGGGCTCTGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCCTCATAAG 
GCCCTCCTGG AGGAAGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG 
AGGGAAAAGT GCGGCTCATA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA 
CAACTGGAGA ACGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGACAGAG GACCCATCTG TTATTGGGGA GTTTAAGGGC 
CTCTTCAAAA TCTATCCCCT CCCAGAAGAC CCGGCTGTTC CCATGCCCCC GAGACAATTC 
CACCAGCTGG CTGCCCAAGG GCCTCAGGAG TGCCTGGTCC GCATCTATAT CATCCGAGCA 
TTTGGCCTTC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATTCCCT GTACTCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACACTC 
TATGACTATG ACCTCCTCTC CAAAGATGAA AAGATTGGGG AGACAGTCAT TGACCTGGAG 
GATAGGCTGC TGTCCAAGTT TGGGGCTCGC TGCGGACTCC CACAGACGTA CTGTGTCTCT 
GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TTCTCCACCT CTTCTGCCAG 
CAGCGCAGAC TCAAGGCTCC TGTGTACCTC ACGGACCACG TGGTGTTCCA GGATAAAGAA 
TACAGCATTG AAGAAATAGA GGCTGGCCGA GTCCCGAACC CACACCTGGG CCCAGTGGAA 
GAGCGTCTGG CCTTGCATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC 
CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC ATTGAACAGG GGAAGCTGCA GATGTGGATC 
GACCTATTTC CCAAGGCCCT GGGGCGTCCC GGACCTCCCT TCAACATCAC CCCGCGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATCATC TGGAACACAA AGGACGTGAT CCTGGACGAC 
CTCAGCATCA CAGGGGAGAA GATGAGTGAT ATTTATGTGA AGGGTTGGAT GGTTGGCTTT 
GAAGAGCACA AGCAAAAGAC AGATGTGCAT TACCGGTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCAGCCG AGCAAGTCTG CACCGTCTCT 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTA 
TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTGGGCTC CTTGCAGTTG 
GATCTCAACC ACATGCCCAA GCCTGCCAAG ACAGCTGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACTT TCCACCCGGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC AGTTAAGGGC 
TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG AAGAAGATCC TGGCAGGCAA GCTGGAAATG 
ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAACCCAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGCC CTGACACCTC CTTCCTCTGG 
TTCACCTCTC CATACAAGAC TATGAAGTTC ATCCTGTGGC GGCGGTTCCG ATGTGCCATT 
ATCCTCTTCA TCATTCTCTT CATCCTGCTA CTATTCTTAG GCATCTTCAT CTATGCTTTC 
CCGAACTACG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260554.1
CDS	406..6867
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEELCAPAFFLQGLLQTSEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHKALLEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404846.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404846.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC 
CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC 
AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC 
CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT 
GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG 
ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA 
GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA 
GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC 
TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT 
GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT 
CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC 
CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC 
CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC 
AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG 
GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC 
CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT 
GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC 
TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT 
GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT 
GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT 
TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT 
GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG 
CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG 
GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGCTGTG TGCACCTGCC 
TTCTTCCTCC AGGGCCTCCT CCAAACGTCA GAGGACATAG ACCTGCCCTA CCCGCCACCT 
CAGAGGGAGG CCAACATCTT CATGGTCCCC CAGAACATCA AGCCTGTTCT CCAGCGTACT 
GCCATAGAGA TCCTGGCGTG GGGCCTGCGG AACATGAAGA GCTACCAGCT AGCCAGCATC 
TCCTCCCCTA GCCTTGTGGT AGAGTGCGGG GGCCAGACAG TGCAGTCCTG TGTCATCAAG 
AACCTCCGGA AGAACCCCAA CTTCGACATC TGTACCCTTT TCATGGAAGT GATGCTGCCC 
AAGGAGGAGG TCTACTGTCC CTCCATCGTG ATCAAGGTCA TTGATAACCG CCAGTTTGGC 
CGCCGGCCTG TGGTGGGCCA GTGTACCATC CGTTCCCTGG AGAGCTTCAT GTGTGACCTG 
TACTCCGAGG AGAATCCGTC CCCACAGGGC GGCCCAGATG ATGTGAGTTT ACTCAGTCCT 
GGGGAAGACG TGCTCATTGA CATCGATGAC AAGGAGCCCC TCATCCCCAT GCAGCTTGCA 
GATGGTCTGT CGGGCTCTGC CCCCACTAAC ATGGCATCTT CTCCATCCAG TCCTCATAAG 
GCCCTCCTGG AGGAAGAGTT CATCGACTGG TGGAGCAAAT TCTTTGCCTC CACAGGGGAG 
AGGGAAAAGT GCGGCTCATA CTTGGAGAAG GGCTTTGACA CTCTAAAGGT CTATGACACA 
CAACTGGAGA ACGTGGAGGC CTTTGAGGGC CTGTCTGACT TTTGTAACAC CTTCAAGCTC 
TACCGGGGCA AGACTCAGGA GGAGACAGAG GACCCATCTG TTATTGGGGA GTTTAAGGGC 
CTCTTCAAAA TCTATCCCCT CCCAGAAGAC CCGGCTGTTC CCATGCCCCC GAGACAATTC 
CACCAGCTGG CTGCCCAAGG GCCTCAGGAG TGCCTGGTCC GCATCTATAT CATCCGAGCA 
TTTGGCCTTC AGCCCAAGGA CCCCAATGGG AAGTGTGATC CTTACATCAA GATCTCCATA 
GGGAAGAAAT CAGTGAGTGA CCAGGATAAC TACATTCCCT GTACTCTGGA GCCTGTGTTT 
GGAAAGATGT TCGAGCTGAC CTGCACTCTG CCTCTGGAGA AGGACCTGAA GATCACACTC 
TATGACTATG ACCTCCTCTC CAAAGATGAA AAGATTGGGG AGACAGTCAT TGACCTGGAG 
GATAGGCTGC TGTCCAAGTT TGGGGCTCGC TGCGGACTCC CACAGACGTA CTGTGTCTCT 
GGACCCAACC AGTGGAGGGA CCAGCTCCGC CCCTCCCAGC TTCTCCACCT CTTCTGCCAG 
CAGCGCAGAC TCAAGGCTCC TGTGTACCTC ACGGACCACG TGGTGTTCCA GGATAAAGAA 
TACAGCATTG AAGAAATAGA GGCTGGCCGA GTCCCGAACC CACACCTGGG CCCAGTGGAA 
GAGCGTCTGG CCTTGCATGT CCTCCAGCAG CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC 
CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC ATTGAACAGG GGAAGCTGCA GATGTGGATC 
GACCTATTTC CCAAGGCCCT GGGGCGTCCC GGACCTCCCT TCAACATCAC CCCGCGGAGA 
GCCAGAAGGT TTTTCCTGCG TTGTATCATC TGGAACACAA AGGACGTGAT CCTGGACGAC 
CTCAGCATCA CAGGGGAGAA GATGAGTGAT ATTTATGTGA AGGGTTGGAT GGTTGGCTTT 
GAAGAGCACA AGCAAAAGAC AGATGTGCAT TACCGGTCCC TGGGAGGTGA AGGCAACTTT 
AACTGGAGGT TCATTTTCCC CTTTGACTAC CTGCCAGCCG AGCAAGTCTG CACCGTCTCT 
AAGAAGGATG CTTTCTGGAG GCTGGACAAG ACTGAGAGCA AAATCCCAGC ACGAGTGGTA 
TTTCAGATCT GGGACAATGA CAAGTTCTCC TTTGACGACT TTCTGGGCTC CTTGCAGTTG 
GATCTCAACC ACATGCCCAA GCCTGCCAAG ACAGCTGAGA AGTGCTCCTT GGACCAGCTG 
GATGACACTT TCCACCCGGA GTGGTTTGTG TCCCTTTTTG AACAGAAAAC AGTTAAGGGC 
TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG AAGAAGATCC TGGCAGGCAA GCTGGAAATG 
ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT 
GAACCCAACA TGAACCCCAA GCTCGAGGAC CCAAGGCGCC CTGACACCTC CTTCCTCTGG 
TTCACCTCTC CATACAAGAC TATGAAGTTC ATCCTGTGGC GGCGGTTCCG ATGTGCCATT 
ATCCTCTTCA TCATTCTCTT CATCCTGCTA CTATTCTTAG GCATCTTCAT CTATGCTTTC 
CCGAACTACG CTGCCATGAA GCTGGTGAAG CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_022404847.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260555.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	OCe294456
Clone ID Related Accession (Same CDS sequence)	XM_022404847.1
Accession Version	XM_022404847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGGAGGAGA GGAGGACACT GAGGACCAGG GGCTCACTGG AGACGAGGGA 
GAGCCATTAC TGGATCAGAG TGGCGTCCCA GGCCCTGGGG CTCCCACCAC CCCGAGGAAG 
CCACCTTCCC ATCTGCCCCC CTACCACCCT GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA 
GCCAGAAAGC TGCTGTCCGA CAAACCGCAG GACTTCCAGA TCAGAGTGCA AGTGATCGAG 
GGCCGCCAGC TGCCAGGGGT GAATATCAAG CCTGTGATCA AGGTCACTGC TGCTGGGCAG 
ACCAAGAGGA CACGGATCCA CAAGGGAAAC AGCCCACTAT TTAACGAGAC TCTTTTCTTC 
AATGTGTTTG ACTCTCCTTC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTCGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCATT GGGGAATTCC GGATGGATGT GGGTACCATC 
TACAGAGAGC CCCGACATGC TTATCTGAGG AAGTGGCTGC TACTCTCCAA CCCTGATGAC 
TTCTCTGTTG GGGCCAGAGG TTACCTGAAA GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT 
GAAGCGCCTC TGGAGAGAAA GGACCCCTCT GAAGACAAGG AGGACATTGA AAACAATCTG 
CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGAT CTTCAGAGCG 
GAGGACTTAC CACAAATGGA TGATGCCGTG ATGGACAATG TGAAGCAGAT CTTTGGCTTT 
GACAGCAACA AGAAGAATTT GGTAGATCCC TTCATGGAAG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCCTGGA GAAGACAGCC AACCCTCAGT GGAACCAGAG CATCACTCTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAAGATTC GCGTCATGGA CTGGGACCGC 
CTTACCCACA ATGACATCGT GGCCACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GTGGTCAAGC CTTCTCCAGC CACGGACCTG 
GATGACCATC TGGGCTTCCT TCCCACCTTC GGGCCCTGCT TCGTCAATCT CTATGGCAGC 
CCCAGGGAAT TCACTGGCTT CCCGGACCCC TACGCGGAAC TCAACATGGG CAAGGGGGAA 
GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGC 
GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT GATGACATCC TTCGGGTGGA GAAATACCTC 
CGGAGGCGCA AGTACTCTCT CTTTGCCGCC TTCTACTCGG CCACCATGCT CCAGGATGTC 
GACGATGCCA TCCAGTTTGA GGTCAGCATT GGGAACTATG GCAACAAGTT CGACACGACC 
TGTCTGCCAC TGGCCTCCAC CACCCAGTAC AGCCGGGCAG TCTTTGATGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA TGTGAAGCCT GTGGTGGTGC TGTCATCCTA CTGGGAAGAC 
ATCAGCCACA GAGTCGAGGC TCAGAACCAG CTGCTCAGGA TCGCTGACCA TCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCTTTAAAG GCGCAGTTCA ACACAGAGGA TGTGGACTCC 
CTGTTGGCCC AGCTGATGGA TGAGCTCATC TCAGACTGCA GCCAGCCCCT GGGCAATGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CGGCACCTGT ACAGGCTGCG CACCCGTCAC 
CTAGGCCAGA TTACTGAGGC GGCCCTGGCT CTGAAACTCG GGCATGGCAA GCTCCCTGCC 
GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAA ACAAGCGAGT GGCGTACCAG 
CGGGTGCCTG CGCACGAGGT CCTCTTCTCC CGGCGGGGCG CCAGCTACTG TGGCAAGAAC 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCCATGG AGGGGGTGCC CGGAGCCCGG 
ATGCCAGTAC AAATACGGGT GAAACTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CTTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ATGGGCAAGA TCAAGCTACC CAAGGATAGC TTCCGCCCCT CAGCTGGCTG GACCTGGGCC 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ACATGGACGC GGGCCACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACAGA TGTGAATGGG GATAAGGTGC TTCCTAAGGA TGACATTGAG 
TGCCCACTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTC 
GATGAGCAAG GCTGGGAGTA CAGTATCACC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA TTACACGCAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA 
AGGGACCTCA GCCAGATGGA GGCATTGAAG AGGCACAGAC AGGCAGAGGC AGAGGGTGAG 
GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG AAGTTCCACC TGGAGTACCG CAAGACTGAT 
GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAACCGC TGGAGAAGAC GGGGCCTGCA 
GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT GGTGTGGTAG ATGACAGGAG TGAAGATTCC 
GTGTCCGTGT CCACCTTGAG CTTTGGAGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CTCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACAGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACGCTTAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGTATC GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ACGACACCTA CGGTGCAGAT GAGTTTATGG GACGCTGCAT TTGTCAGCCC 
AGCCTGGAGC GGACACCCCG GCTGGCCTGG TTCCCACTGA TTAGGGGCAG CCAGTCGGCG 
GGGGAACTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAAA AGCCGGCCAT TCACCATATT 
CCTGGTTTTG AGGTACAGGA TATGTCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCAGAG GACATAGACC TGCCCTACCC GCCACCTCAG 
AGGGAGGCCA ACATCTTCAT GGTCCCCCAG AACATCAAGC CTGTTCTCCA GCGTACTGCC 
ATAGAGATCC TGGCGTGGGG CCTGCGGAAC ATGAAGAGCT ACCAGCTAGC CAGCATCTCC 
TCCCCTAGCC TTGTGGTAGA GTGCGGGGGC CAGACAGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT CGACATCTGT ACCCTTTTCA TGGAAGTGAT GCTGCCCAAG 
GAGGAGGTCT ACTGTCCCTC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGT TCCCTGGAGA GCTTCATGTG TGACCTGTAC 
TCCGAGGAGA ATCCGTCCCC ACAGGGCGGC CCAGATGATG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT CGATGACAAG GAGCCCCTCA TCCCCATGCA GCTTGCAGAT 
GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCTTCTC CATCCAGTCC TCATAAGGCC 
CTCCTGGAGG AAGAGTTCAT CGACTGGTGG AGCAAATTCT TTGCCTCCAC AGGGGAGAGG 
GAAAAGTGCG GCTCATACTT GGAGAAGGGC TTTGACACTC TAAAGGTCTA TGACACACAA 
CTGGAGAACG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTCTAC 
CGGGGCAAGA CTCAGGAGGA GACAGAGGAC CCATCTGTTA TTGGGGAGTT TAAGGGCCTC 
TTCAAAATCT ATCCCCTCCC AGAAGACCCG GCTGTTCCCA TGCCCCCGAG ACAATTCCAC 
CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC CTGGTCCGCA TCTATATCAT CCGAGCATTT 
GGCCTTCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGAT CACACTCTAT 
GACTATGACC TCCTCTCCAA AGATGAAAAG ATTGGGGAGA CAGTCATTGA CCTGGAGGAT 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC GGACTCCCAC AGACGTACTG TGTCTCTGGA 
CCCAACCAGT GGAGGGACCA GCTCCGCCCC TCCCAGCTTC TCCACCTCTT CTGCCAGCAG 
CGCAGACTCA AGGCTCCTGT GTACCTCACG GACCACGTGG TGTTCCAGGA TAAAGAATAC 
AGCATTGAAG AAATAGAGGC TGGCCGAGTC CCGAACCCAC ACCTGGGCCC AGTGGAAGAG 
CGTCTGGCCT TGCATGTCCT CCAGCAGCAG GGCCTGGTCC CTGAGCATGT GGAGTCCCGG 
CCTCTCTACA GCCCCCTGCA GCCAGACATT GAACAGGGGA AGCTGCAGAT GTGGATCGAC 
CTATTTCCCA AGGCCCTGGG GCGTCCCGGA CCTCCCTTCA ACATCACCCC GCGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATCATCTGG AACACAAAGG ACGTGATCCT GGACGACCTC 
AGCATCACAG GGGAGAAGAT GAGTGATATT TATGTGAAGG GTTGGATGGT TGGCTTTGAA 
GAGCACAAGC AAAAGACAGA TGTGCATTAC CGGTCCCTGG GAGGTGAAGG CAACTTTAAC 
TGGAGGTTCA TTTTCCCCTT TGACTACCTG CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG 
AAGGATGCTT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTATTT 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GACGACTTTC TGGGCTCCTT GCAGTTGGAT 
CTCAACCACA TGCCCAAGCC TGCCAAGACA GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTTCC ACCCGGAGTG GTTTGTGTCC CTTTTTGAAC AGAAAACAGT TAAGGGCTGG 
TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG AAGATCCTGG CAGGCAAGCT GGAAATGACC 
TTGGAGATCG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAA 
CCCAACATGA ACCCCAAGCT CGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTCTGGTTC 
ACCTCTCCAT ACAAGACTAT GAAGTTCATC CTGTGGCGGC GGTTCCGATG TGCCATTATC 
CTCTTCATCA TTCTCTTCAT CCTGCTACTA TTCTTAGGCA TCTTCATCTA TGCTTTCCCG 
AACTACGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260555.1
CDS	23..6481
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEELCAPAFFLQGLLQTSEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHKALLEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404847.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404847.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGGAGGAGA GGAGGACACT GAGGACCAGG GGCTCACTGG AGACGAGGGA 
GAGCCATTAC TGGATCAGAG TGGCGTCCCA GGCCCTGGGG CTCCCACCAC CCCGAGGAAG 
CCACCTTCCC ATCTGCCCCC CTACCACCCT GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA 
GCCAGAAAGC TGCTGTCCGA CAAACCGCAG GACTTCCAGA TCAGAGTGCA AGTGATCGAG 
GGCCGCCAGC TGCCAGGGGT GAATATCAAG CCTGTGATCA AGGTCACTGC TGCTGGGCAG 
ACCAAGAGGA CACGGATCCA CAAGGGAAAC AGCCCACTAT TTAACGAGAC TCTTTTCTTC 
AATGTGTTTG ACTCTCCTTC GGAGCTGTTT GATGAGCCCA TCTTTATCAC GGTCGTAGAC 
TCCCGTTCTC TCAGGACAGA TGCTCTCATT GGGGAATTCC GGATGGATGT GGGTACCATC 
TACAGAGAGC CCCGACATGC TTATCTGAGG AAGTGGCTGC TACTCTCCAA CCCTGATGAC 
TTCTCTGTTG GGGCCAGAGG TTACCTGAAA GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT 
GAAGCGCCTC TGGAGAGAAA GGACCCCTCT GAAGACAAGG AGGACATTGA AAACAATCTG 
CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA GCCCACTTCT GCCTGAAGAT CTTCAGAGCG 
GAGGACTTAC CACAAATGGA TGATGCCGTG ATGGACAATG TGAAGCAGAT CTTTGGCTTT 
GACAGCAACA AGAAGAATTT GGTAGATCCC TTCATGGAAG TCAGCTTTGC GGGGAAAATG 
CTCTGCAGCA AGATCCTGGA GAAGACAGCC AACCCTCAGT GGAACCAGAG CATCACTCTG 
CCTGCCATGT TTCCCTCCAT GTGTGAAAAA ATGAAGATTC GCGTCATGGA CTGGGACCGC 
CTTACCCACA ATGACATCGT GGCCACCACC TACCTGAGTA TGTCGAAAAT CTCTGCCCCT 
GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT GTGGTCAAGC CTTCTCCAGC CACGGACCTG 
GATGACCATC TGGGCTTCCT TCCCACCTTC GGGCCCTGCT TCGTCAATCT CTATGGCAGC 
CCCAGGGAAT TCACTGGCTT CCCGGACCCC TACGCGGAAC TCAACATGGG CAAGGGGGAA 
GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC TCCCTGGAGA CCAAGCTGGT GGAGCACAGC 
GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT GATGACATCC TTCGGGTGGA GAAATACCTC 
CGGAGGCGCA AGTACTCTCT CTTTGCCGCC TTCTACTCGG CCACCATGCT CCAGGATGTC 
GACGATGCCA TCCAGTTTGA GGTCAGCATT GGGAACTATG GCAACAAGTT CGACACGACC 
TGTCTGCCAC TGGCCTCCAC CACCCAGTAC AGCCGGGCAG TCTTTGATGG GTGCCACTAC 
TACTACTTGC CCTGGGGCAA TGTGAAGCCT GTGGTGGTGC TGTCATCCTA CTGGGAAGAC 
ATCAGCCACA GAGTCGAGGC TCAGAACCAG CTGCTCAGGA TCGCTGACCA TCTGGAAGCT 
GGCCTGGAGC AAGTCCACTT GGCTTTAAAG GCGCAGTTCA ACACAGAGGA TGTGGACTCC 
CTGTTGGCCC AGCTGATGGA TGAGCTCATC TCAGACTGCA GCCAGCCCCT GGGCAATGTC 
CAAGAGATAC CCTCTGCCAC CCACCTGGAC CGGCACCTGT ACAGGCTGCG CACCCGTCAC 
CTAGGCCAGA TTACTGAGGC GGCCCTGGCT CTGAAACTCG GGCATGGCAA GCTCCCTGCC 
GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG 
AACAGCCTGC CCGACATCAT CATCTGGATG CTGCAGGGAA ACAAGCGAGT GGCGTACCAG 
CGGGTGCCTG CGCACGAGGT CCTCTTCTCC CGGCGGGGCG CCAGCTACTG TGGCAAGAAC 
TGTGGGAAGC TGCAGACGAT ATTTCTGAAA TATCCCATGG AGGGGGTGCC CGGAGCCCGG 
ATGCCAGTAC AAATACGGGT GAAACTCTGG TTTGGGCTTT CTGTGGATGA GAAGGAGTTC 
AACCAGTTTG CTGAGGGAAA GCTGTCCGTC TTTGCTGAAA CTTATGAGAA CCAGACCAAA 
CTGGCCCTGG TTGGGAACTG GGGCACAACA GGCCTCACCT ACCCCAAGTT TTCTGATGTC 
ATGGGCAAGA TCAAGCTACC CAAGGATAGC TTCCGCCCCT CAGCTGGCTG GACCTGGGCC 
GGAGACTGGT TCGTGTGTCC AGAGAAGACC CTACTCCATG ACATGGACGC GGGCCACCTG 
AGTTTTGTGG AGGAGGTGTT TGAGAACCAG ACCCGGCTCC CTGGAGGCCA GTGGATCTAC 
ATGAGTGACA ACTACACAGA TGTGAATGGG GATAAGGTGC TTCCTAAGGA TGACATTGAG 
TGCCCACTGG GCTGGAAGTG GGAAGATGAA GAGTGGTCCA CGGACCTCAA TCGGGCCGTC 
GATGAGCAAG GCTGGGAGTA CAGTATCACC ATCCCCCCGG ATCGGAAGCC AAAGCACTGG 
GTTCCTGCTG AGAAGATGTA TTACACGCAC CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA 
AGGGACCTCA GCCAGATGGA GGCATTGAAG AGGCACAGAC AGGCAGAGGC AGAGGGTGAG 
GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG AAGTTCCACC TGGAGTACCG CAAGACTGAT 
GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC ATGGAACCGC TGGAGAAGAC GGGGCCTGCA 
GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT GGTGTGGTAG ATGACAGGAG TGAAGATTCC 
GTGTCCGTGT CCACCTTGAG CTTTGGAGTG AACAGACCCA CGATTTCCTG CATCTTTGAT 
TATGGGAACC GCTACCACCT ACGCTGCTAC ATGTACCAGG CTCGGGACCT GCCCTCGATG 
GACAAGGACT CTTTTTCTGA TCCCTACGCC ATCGTCTCCT TCCTGCACCA GAGCCAGAAG 
ACAGTGGTGG CGAAGAACAC TCTGAACCCC ACCTGGGACC AGACGCTTAT CTTCTATGAG 
ATTGAGATCT TTGGTGAGCC AGCCAGTATC GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG 
CTGTATGACC ACGACACCTA CGGTGCAGAT GAGTTTATGG GACGCTGCAT TTGTCAGCCC 
AGCCTGGAGC GGACACCCCG GCTGGCCTGG TTCCCACTGA TTAGGGGCAG CCAGTCGGCG 
GGGGAACTGC TGGCCTCTTT TGAGCTCATC CAGAGAGAAA AGCCGGCCAT TCACCATATT 
CCTGGTTTTG AGGTACAGGA TATGTCAGGA ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC 
TTCCTCCAGG GCCTCCTCCA AACGTCAGAG GACATAGACC TGCCCTACCC GCCACCTCAG 
AGGGAGGCCA ACATCTTCAT GGTCCCCCAG AACATCAAGC CTGTTCTCCA GCGTACTGCC 
ATAGAGATCC TGGCGTGGGG CCTGCGGAAC ATGAAGAGCT ACCAGCTAGC CAGCATCTCC 
TCCCCTAGCC TTGTGGTAGA GTGCGGGGGC CAGACAGTGC AGTCCTGTGT CATCAAGAAC 
CTCCGGAAGA ACCCCAACTT CGACATCTGT ACCCTTTTCA TGGAAGTGAT GCTGCCCAAG 
GAGGAGGTCT ACTGTCCCTC CATCGTGATC AAGGTCATTG ATAACCGCCA GTTTGGCCGC 
CGGCCTGTGG TGGGCCAGTG TACCATCCGT TCCCTGGAGA GCTTCATGTG TGACCTGTAC 
TCCGAGGAGA ATCCGTCCCC ACAGGGCGGC CCAGATGATG TGAGTTTACT CAGTCCTGGG 
GAAGACGTGC TCATTGACAT CGATGACAAG GAGCCCCTCA TCCCCATGCA GCTTGCAGAT 
GGTCTGTCGG GCTCTGCCCC CACTAACATG GCATCTTCTC CATCCAGTCC TCATAAGGCC 
CTCCTGGAGG AAGAGTTCAT CGACTGGTGG AGCAAATTCT TTGCCTCCAC AGGGGAGAGG 
GAAAAGTGCG GCTCATACTT GGAGAAGGGC TTTGACACTC TAAAGGTCTA TGACACACAA 
CTGGAGAACG TGGAGGCCTT TGAGGGCCTG TCTGACTTTT GTAACACCTT CAAGCTCTAC 
CGGGGCAAGA CTCAGGAGGA GACAGAGGAC CCATCTGTTA TTGGGGAGTT TAAGGGCCTC 
TTCAAAATCT ATCCCCTCCC AGAAGACCCG GCTGTTCCCA TGCCCCCGAG ACAATTCCAC 
CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC CTGGTCCGCA TCTATATCAT CCGAGCATTT 
GGCCTTCAGC CCAAGGACCC CAATGGGAAG TGTGATCCTT ACATCAAGAT CTCCATAGGG 
AAGAAATCAG TGAGTGACCA GGATAACTAC ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA 
AAGATGTTCG AGCTGACCTG CACTCTGCCT CTGGAGAAGG ACCTGAAGAT CACACTCTAT 
GACTATGACC TCCTCTCCAA AGATGAAAAG ATTGGGGAGA CAGTCATTGA CCTGGAGGAT 
AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC GGACTCCCAC AGACGTACTG TGTCTCTGGA 
CCCAACCAGT GGAGGGACCA GCTCCGCCCC TCCCAGCTTC TCCACCTCTT CTGCCAGCAG 
CGCAGACTCA AGGCTCCTGT GTACCTCACG GACCACGTGG TGTTCCAGGA TAAAGAATAC 
AGCATTGAAG AAATAGAGGC TGGCCGAGTC CCGAACCCAC ACCTGGGCCC AGTGGAAGAG 
CGTCTGGCCT TGCATGTCCT CCAGCAGCAG GGCCTGGTCC CTGAGCATGT GGAGTCCCGG 
CCTCTCTACA GCCCCCTGCA GCCAGACATT GAACAGGGGA AGCTGCAGAT GTGGATCGAC 
CTATTTCCCA AGGCCCTGGG GCGTCCCGGA CCTCCCTTCA ACATCACCCC GCGGAGAGCC 
AGAAGGTTTT TCCTGCGTTG TATCATCTGG AACACAAAGG ACGTGATCCT GGACGACCTC 
AGCATCACAG GGGAGAAGAT GAGTGATATT TATGTGAAGG GTTGGATGGT TGGCTTTGAA 
GAGCACAAGC AAAAGACAGA TGTGCATTAC CGGTCCCTGG GAGGTGAAGG CAACTTTAAC 
TGGAGGTTCA TTTTCCCCTT TGACTACCTG CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG 
AAGGATGCTT TCTGGAGGCT GGACAAGACT GAGAGCAAAA TCCCAGCACG AGTGGTATTT 
CAGATCTGGG ACAATGACAA GTTCTCCTTT GACGACTTTC TGGGCTCCTT GCAGTTGGAT 
CTCAACCACA TGCCCAAGCC TGCCAAGACA GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT 
GACACTTTCC ACCCGGAGTG GTTTGTGTCC CTTTTTGAAC AGAAAACAGT TAAGGGCTGG 
TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG AAGATCCTGG CAGGCAAGCT GGAAATGACC 
TTGGAGATCG TAGCAGAGAG TGAGCATGAG GAGCGGCCTG CTGGCCAGGG CCGGGATGAA 
CCCAACATGA ACCCCAAGCT CGAGGACCCA AGGCGCCCTG ACACCTCCTT CCTCTGGTTC 
ACCTCTCCAT ACAAGACTAT GAAGTTCATC CTGTGGCGGC GGTTCCGATG TGCCATTATC 
CTCTTCATCA TTCTCTTCAT CCTGCTACTA TTCTTAGGCA TCTTCATCTA TGCTTTCCCG 
AACTACGCTG CCATGAAGCT GGTGAAGCCC TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_022404848.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260556.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	OCe294457
Clone ID Related Accession (Same CDS sequence)	XM_022404848.1
Accession Version	XM_022404848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT 
GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT 
GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260556.1
CDS	23..6475
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEELCAPAFFLQGLLQTSEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404848.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404848.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT 
GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT 
GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_022404849.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260557.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	OCe294458
Clone ID Related Accession (Same CDS sequence)	XM_022404849.1
Accession Version	XM_022404849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA 
GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC 
AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG 
AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA 
GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT 
TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC 
ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG 
GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT 
GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC 
CTCATCCCCA TGCAGCTTGC AGATGGTCTG TCGGGCTCTG CCCCCACTAA CATGGCATCT 
TCTCCATCCA GTCCTCATAA GGCCCTCCTG GAGGAAGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT 
GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT 
CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC 
CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC 
CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC 
GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC 
TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG 
AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG 
CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA 
GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260557.1
CDS	23..6445
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHKALLEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404849.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404849.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA 
GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC 
AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG 
AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA 
GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT 
TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC 
ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG 
GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT 
GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC 
CTCATCCCCA TGCAGCTTGC AGATGGTCTG TCGGGCTCTG CCCCCACTAA CATGGCATCT 
TCTCCATCCA GTCCTCATAA GGCCCTCCTG GAGGAAGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT 
GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT 
CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC 
CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC 
CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC 
GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC 
TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG 
AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG 
CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA 
GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_022404850.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260558.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	OCe294459
Clone ID Related Accession (Same CDS sequence)	XM_022404850.1
Accession Version	XM_022404850.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGCTGT GTGCACCTGC CTTCTTCCTC CAGGGCCTCC TCCAAACGTC AGAGGACATA 
GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC 
AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG 
AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA 
GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT 
TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC 
ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG 
GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT 
GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC 
CTCATCCCCA TGCAGCTTGC AGATGGTCTG TCGGGCTCTG CCCCCACTAA CATGGCATCT 
TCTCCATCCA GTCCTCATAA GGCCCTCCTG GAGGAAGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT 
GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT 
CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC 
CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC 
CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC 
GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC 
TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG 
AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG 
CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA 
GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260558.1
CDS	23..6445
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEELCAPAFFLQGLLQTSEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHKALLEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404850.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404850.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGCTGT GTGCACCTGC CTTCTTCCTC CAGGGCCTCC TCCAAACGTC AGAGGACATA 
GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC 
AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG 
AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA 
GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT 
TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC 
ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG 
GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT 
GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC 
CTCATCCCCA TGCAGCTTGC AGATGGTCTG TCGGGCTCTG CCCCCACTAA CATGGCATCT 
TCTCCATCCA GTCCTCATAA GGCCCTCCTG GAGGAAGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT 
GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT 
CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC 
CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC 
CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC 
GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC 
TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG 
AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG 
CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA 
GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_022404851.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260559.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	OCe294460
Clone ID Related Accession (Same CDS sequence)	XM_022404851.1
Accession Version	XM_022404851.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT 
GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG 
GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC 
GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC 
TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT 
GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC 
CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC 
AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA 
GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC 
AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA 
GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG 
CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC 
GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA 
TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC 
ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260559.1
CDS	23..6412
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEELCAPAFFLQGLLQTSEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404851.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404851.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGCT GTGTGCACCT 
GCCTTCTTCC TCCAGGGCCT CCTCCAAACG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG 
GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC 
GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC 
TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT 
GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC 
CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC 
AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA 
GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC 
AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA 
GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG 
CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC 
GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA 
TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC 
ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_022404852.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260560.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	OCe294461
Clone ID Related Accession (Same CDS sequence)	XM_022404852.1
Accession Version	XM_022404852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGCTGTG TGCACCTGCC TTCTTCCTCC AGGGCCTCCT CCAAACGTCA 
GAGGACATAG ACCTGCCCTA CCCGCCACCT CAGAGGGAGG CCAACATCTT CATGGTCCCC 
CAGAACATCA AGCCTGTTCT CCAGCGTACT GCCATAGAGA TCCTGGCGTG GGGCCTGCGG 
AACATGAAGA GCTACCAGCT AGCCAGCATC TCCTCCCCTA GCCTTGTGGT AGAGTGCGGG 
GGCCAGACAG TGCAGTCCTG TGTCATCAAG AACCTCCGGA AGAACCCCAA CTTCGACATC 
TGTACCCTTT TCATGGAAGT GATGCTGCCC AAGGAGGAGG TCTACTGTCC CTCCATCGTG 
ATCAAGGTCA TTGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGTTCCCTGG AGAGCTTCAT GTGTGACCTG TACTCCGAGG AGAATCCGTC CCCACAGGGC 
GGCCCAGATG ATGTGAGTTT ACTCAGTCCT GGGGAAGACG TGCTCATTGA CATCGATGAC 
AAGGAGCCCC TCATCCCCAT GCAGCTTGCA GATGGTCTGT CGGGCTCTGC CCCCACTAAC 
ATGGCATCTT CTCCATCCAG TCCTCATAAG GCCCTCCTGG AGGAAGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG 
GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC 
CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG 
TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA 
AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC 
ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA 
GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC 
ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC 
GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT 
ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT 
TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG 
AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT 
GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA 
CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260560.1
CDS	23..6394
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEELCAPAFFLQGLLQTSEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHKALLEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404852.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404852.1

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGCTGTG TGCACCTGCC TTCTTCCTCC AGGGCCTCCT CCAAACGTCA 
GAGGACATAG ACCTGCCCTA CCCGCCACCT CAGAGGGAGG CCAACATCTT CATGGTCCCC 
CAGAACATCA AGCCTGTTCT CCAGCGTACT GCCATAGAGA TCCTGGCGTG GGGCCTGCGG 
AACATGAAGA GCTACCAGCT AGCCAGCATC TCCTCCCCTA GCCTTGTGGT AGAGTGCGGG 
GGCCAGACAG TGCAGTCCTG TGTCATCAAG AACCTCCGGA AGAACCCCAA CTTCGACATC 
TGTACCCTTT TCATGGAAGT GATGCTGCCC AAGGAGGAGG TCTACTGTCC CTCCATCGTG 
ATCAAGGTCA TTGATAACCG CCAGTTTGGC CGCCGGCCTG TGGTGGGCCA GTGTACCATC 
CGTTCCCTGG AGAGCTTCAT GTGTGACCTG TACTCCGAGG AGAATCCGTC CCCACAGGGC 
GGCCCAGATG ATGTGAGTTT ACTCAGTCCT GGGGAAGACG TGCTCATTGA CATCGATGAC 
AAGGAGCCCC TCATCCCCAT GCAGCTTGCA GATGGTCTGT CGGGCTCTGC CCCCACTAAC 
ATGGCATCTT CTCCATCCAG TCCTCATAAG GCCCTCCTGG AGGAAGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG 
GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC 
CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG 
TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA 
AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC 
ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA 
GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC 
ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC 
GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT 
ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT 
TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG 
AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT 
GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA 
CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_022404853.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260561.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	OCe294462
Clone ID Related Accession (Same CDS sequence)	XM_022404853.1
Accession Version	XM_022404853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTCCA AACGTCAGAG 
GACATAGACC TGCCCTACCC GCCACCTCAG AGGGAGGCCA ACATCTTCAT GGTCCCCCAG 
AACATCAAGC CTGTTCTCCA GCGTACTGCC ATAGAGATCC TGGCGTGGGG CCTGCGGAAC 
ATGAAGAGCT ACCAGCTAGC CAGCATCTCC TCCCCTAGCC TTGTGGTAGA GTGCGGGGGC 
CAGACAGTGC AGTCCTGTGT CATCAAGAAC CTCCGGAAGA ACCCCAACTT CGACATCTGT 
ACCCTTTTCA TGGAAGTGAT GCTGCCCAAG GAGGAGGTCT ACTGTCCCTC CATCGTGATC 
AAGGTCATTG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGT 
TCCCTGGAGA GCTTCATGTG TGACCTGTAC TCCGAGGAGA ATCCGTCCCC ACAGGGCGGC 
CCAGATGATG TGAGTTTACT CAGTCCTGGG GAAGACGTGC TCATTGACAT CGATGACAAG 
GAGCCCCTCA TCCCCATGCA GCTTGCAGAT GGTCTGTCGG GCTCTGCCCC CACTAACATG 
GCATCTTCTC CATCCAGTCC TCATAAGGCC CTCCTGGAGG AAGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC 
CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG 
GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC 
CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG 
ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC 
GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG 
GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC 
CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT 
GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA 
CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT 
TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC 
CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG 
AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA 
TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260561.1
CDS	406..6774
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEELCAPAFFLQGLLQTSEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHKALLEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404853.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404853.1

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGCTGTGTGC ACCTGCCTTC TTCCTCCAGG GCCTCCTCCA AACGTCAGAG 
GACATAGACC TGCCCTACCC GCCACCTCAG AGGGAGGCCA ACATCTTCAT GGTCCCCCAG 
AACATCAAGC CTGTTCTCCA GCGTACTGCC ATAGAGATCC TGGCGTGGGG CCTGCGGAAC 
ATGAAGAGCT ACCAGCTAGC CAGCATCTCC TCCCCTAGCC TTGTGGTAGA GTGCGGGGGC 
CAGACAGTGC AGTCCTGTGT CATCAAGAAC CTCCGGAAGA ACCCCAACTT CGACATCTGT 
ACCCTTTTCA TGGAAGTGAT GCTGCCCAAG GAGGAGGTCT ACTGTCCCTC CATCGTGATC 
AAGGTCATTG ATAACCGCCA GTTTGGCCGC CGGCCTGTGG TGGGCCAGTG TACCATCCGT 
TCCCTGGAGA GCTTCATGTG TGACCTGTAC TCCGAGGAGA ATCCGTCCCC ACAGGGCGGC 
CCAGATGATG TGAGTTTACT CAGTCCTGGG GAAGACGTGC TCATTGACAT CGATGACAAG 
GAGCCCCTCA TCCCCATGCA GCTTGCAGAT GGTCTGTCGG GCTCTGCCCC CACTAACATG 
GCATCTTCTC CATCCAGTCC TCATAAGGCC CTCCTGGAGG AAGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC 
CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG 
GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC 
CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG 
ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC 
GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG 
GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC 
CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT 
GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA 
CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT 
TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC 
CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG 
AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA 
TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_022404854.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022260562.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X22, mRNA.

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CloneID	OCe294463
Clone ID Related Accession (Same CDS sequence)	XM_022404854.1
Accession Version	XM_022404854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGGA ACAGGTTCTT AGGGGAAGCC CAAGTCCCAC TCCAGGAGGT CCTCGCCACT 
CCTAGTCTGT CTGCCAGCTT CAATGCCCCC CTCCTGGACG CCAAGAAGCA GCCCACGGGG 
GCCTCGCTGG TCCTGCAGGT GTCCTACACT CCACTCCCTG GAGCCGTGCC CCTGTTCCCA 
CCTGCTGCAT CTCTGGAACC CTCCCCGACT CTGCCTGACA TGGATATGGG GGCCGGTGGG 
GGACAGAGCC GGGCTGAGAC TTGGTCCCTG CTCAGTGACA GCACCGTGGA TACAAGATAC 
TCTGGAAAGA AGTGGCTGGC CCCCACGGAC ACAGGAGGAG AGGAGGACAC TGAGGACCAG 
GGGCTCACTG GAGACGAGGG AGAGCCATTA CTGGATCAGA GTGGCGTCCC AGGCCCTGGG 
GCTCCCACCA CCCCGAGGAA GCCACCTTCC CATCTGCCCC CCTACCACCC TGGGAGCAAG 
AGGAAAAGAA GTGCTCCCGC AGCCAGAAAG CTGCTGTCCG ACAAACCGCA GGACTTCCAG 
ATCAGAGTGC AAGTGATCGA GGGCCGCCAG CTGCCAGGGG TGAATATCAA GCCTGTGATC 
AAGGTCACTG CTGCTGGGCA GACCAAGAGG ACACGGATCC ACAAGGGAAA CAGCCCACTA 
TTTAACGAGA CTCTTTTCTT CAATGTGTTT GACTCTCCTT CGGAGCTGTT TGATGAGCCC 
ATCTTTATCA CGGTCGTAGA CTCCCGTTCT CTCAGGACAG ATGCTCTCAT TGGGGAATTC 
CGGATGGATG TGGGTACCAT CTACAGAGAG CCCCGACATG CTTATCTGAG GAAGTGGCTG 
CTACTCTCCA ACCCTGATGA CTTCTCTGTT GGGGCCAGAG GTTACCTGAA AGCAAGCCTT 
TATGTGCTGG GGCCCGGAGA TGAAGCGCCT CTGGAGAGAA AGGACCCCTC TGAAGACAAG 
GAGGACATTG AAAACAATCT GCTCAGGCCA ACTGGCGTGG CCCTGCGAGG AGCCCACTTC 
TGCCTGAAGA TCTTCAGAGC GGAGGACTTA CCACAAATGG ATGATGCCGT GATGGACAAT 
GTGAAGCAGA TCTTTGGCTT TGACAGCAAC AAGAAGAATT TGGTAGATCC CTTCATGGAA 
GTCAGCTTTG CGGGGAAAAT GCTCTGCAGC AAGATCCTGG AGAAGACAGC CAACCCTCAG 
TGGAACCAGA GCATCACTCT GCCTGCCATG TTTCCCTCCA TGTGTGAAAA AATGAAGATT 
CGCGTCATGG ACTGGGACCG CCTTACCCAC AATGACATCG TGGCCACCAC CTACCTGAGT 
ATGTCGAAAA TCTCTGCCCC TGGAGGAGAA ATAGAAGAGG AGCCTGCAGG TGTGGTCAAG 
CCTTCTCCAG CCACGGACCT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTCC AAACGTCAGA GGACATAGAC 
CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG 
CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC 
TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC 
ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATGC AGCTTGCAGA TGGTCTGTCG GGCTCTGCCC CCACTAACAT GGCATCTTCT 
CCATCCAGTC CTCATAAGGC CCTCCTGGAG GAAGAGTTCA TCGACTGGTG GAGCAAATTC 
TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC GGCTCATACT TGGAGAAGGG CTTTGACACT 
CTAAAGGTCT ATGACACACA ACTGGAGAAC GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGACAGAGGA CCCATCTGTT 
ATTGGGGAGT TTAAGGGCCT CTTCAAAATC TATCCCCTCC CAGAAGACCC GGCTGTTCCC 
ATGCCCCCGA GACAATTCCA CCAGCTGGCT GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC 
ATCTATATCA TCCGAGCATT TGGCCTTCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATTCCCTGT 
ACTCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACACTCTA TGACTATGAC CTCCTCTCCA AAGATGAAAA GATTGGGGAG 
ACAGTCATTG ACCTGGAGGA TAGGCTGCTG TCCAAGTTTG GGGCTCGCTG CGGACTCCCA 
CAGACGTACT GTGTCTCTGG ACCCAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTT 
CTCCACCTCT TCTGCCAGCA GCGCAGACTC AAGGCTCCTG TGTACCTCAC GGACCACGTG 
GTGTTCCAGG ATAAAGAATA CAGCATTGAA GAAATAGAGG CTGGCCGAGT CCCGAACCCA 
CACCTGGGCC CAGTGGAAGA GCGTCTGGCC TTGCATGTCC TCCAGCAGCA GGGCCTGGTC 
CCTGAGCATG TGGAGTCCCG GCCTCTCTAC AGCCCCCTGC AGCCAGACAT TGAACAGGGG 
AAGCTGCAGA TGTGGATCGA CCTATTTCCC AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC 
AACATCACCC CGCGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATCATCTG GAACACAAAG 
GACGTGATCC TGGACGACCT CAGCATCACA GGGGAGAAGA TGAGTGATAT TTATGTGAAG 
GGTTGGATGG TTGGCTTTGA AGAGCACAAG CAAAAGACAG ATGTGCATTA CCGGTCCCTG 
GGAGGTGAAG GCAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCCAGCCGAG 
CAAGTCTGCA CCGTCTCTAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTATT TCAGATCTGG GACAATGACA AGTTCTCCTT TGACGACTTT 
CTGGGCTCCT TGCAGTTGGA TCTCAACCAC ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCGGAGT GGTTTGTGTC CCTTTTTGAA 
CAGAAAACAG TTAAGGGCTG GTGGCCCTGC GTAGCAGAAG AGGGCGAGAA GAAGATCCTG 
GCAGGCAAGC TGGAAATGAC CTTGGAGATC GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA ACCCAACATG AACCCCAAGC TCGAGGACCC AAGGCGCCCT 
GACACCTCCT TCCTCTGGTT CACCTCTCCA TACAAGACTA TGAAGTTCAT CCTGTGGCGG 
CGGTTCCGAT GTGCCATTAT CCTCTTCATC ATTCTCTTCA TCCTGCTACT ATTCTTAGGC 
ATCTTCATCT ATGCTTTCCC GAACTACGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022260562.1
CDS	225..6464
Translation	MGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEELCAPAFFLQGLLQTSEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHKALLEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_022404854.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022404854.1

ORF Insert Sequence:

ATGGGGAGGA ACAGGTTCTT AGGGGAAGCC CAAGTCCCAC TCCAGGAGGT CCTCGCCACT 
CCTAGTCTGT CTGCCAGCTT CAATGCCCCC CTCCTGGACG CCAAGAAGCA GCCCACGGGG 
GCCTCGCTGG TCCTGCAGGT GTCCTACACT CCACTCCCTG GAGCCGTGCC CCTGTTCCCA 
CCTGCTGCAT CTCTGGAACC CTCCCCGACT CTGCCTGACA TGGATATGGG GGCCGGTGGG 
GGACAGAGCC GGGCTGAGAC TTGGTCCCTG CTCAGTGACA GCACCGTGGA TACAAGATAC 
TCTGGAAAGA AGTGGCTGGC CCCCACGGAC ACAGGAGGAG AGGAGGACAC TGAGGACCAG 
GGGCTCACTG GAGACGAGGG AGAGCCATTA CTGGATCAGA GTGGCGTCCC AGGCCCTGGG 
GCTCCCACCA CCCCGAGGAA GCCACCTTCC CATCTGCCCC CCTACCACCC TGGGAGCAAG 
AGGAAAAGAA GTGCTCCCGC AGCCAGAAAG CTGCTGTCCG ACAAACCGCA GGACTTCCAG 
ATCAGAGTGC AAGTGATCGA GGGCCGCCAG CTGCCAGGGG TGAATATCAA GCCTGTGATC 
AAGGTCACTG CTGCTGGGCA GACCAAGAGG ACACGGATCC ACAAGGGAAA CAGCCCACTA 
TTTAACGAGA CTCTTTTCTT CAATGTGTTT GACTCTCCTT CGGAGCTGTT TGATGAGCCC 
ATCTTTATCA CGGTCGTAGA CTCCCGTTCT CTCAGGACAG ATGCTCTCAT TGGGGAATTC 
CGGATGGATG TGGGTACCAT CTACAGAGAG CCCCGACATG CTTATCTGAG GAAGTGGCTG 
CTACTCTCCA ACCCTGATGA CTTCTCTGTT GGGGCCAGAG GTTACCTGAA AGCAAGCCTT 
TATGTGCTGG GGCCCGGAGA TGAAGCGCCT CTGGAGAGAA AGGACCCCTC TGAAGACAAG 
GAGGACATTG AAAACAATCT GCTCAGGCCA ACTGGCGTGG CCCTGCGAGG AGCCCACTTC 
TGCCTGAAGA TCTTCAGAGC GGAGGACTTA CCACAAATGG ATGATGCCGT GATGGACAAT 
GTGAAGCAGA TCTTTGGCTT TGACAGCAAC AAGAAGAATT TGGTAGATCC CTTCATGGAA 
GTCAGCTTTG CGGGGAAAAT GCTCTGCAGC AAGATCCTGG AGAAGACAGC CAACCCTCAG 
TGGAACCAGA GCATCACTCT GCCTGCCATG TTTCCCTCCA TGTGTGAAAA AATGAAGATT 
CGCGTCATGG ACTGGGACCG CCTTACCCAC AATGACATCG TGGCCACCAC CTACCTGAGT 
ATGTCGAAAA TCTCTGCCCC TGGAGGAGAA ATAGAAGAGG AGCCTGCAGG TGTGGTCAAG 
CCTTCTCCAG CCACGGACCT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGCTGTGTG CACCTGCCTT CTTCCTCCAG GGCCTCCTCC AAACGTCAGA GGACATAGAC 
CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG 
CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC 
TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC 
ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATGC AGCTTGCAGA TGGTCTGTCG GGCTCTGCCC CCACTAACAT GGCATCTTCT 
CCATCCAGTC CTCATAAGGC CCTCCTGGAG GAAGAGTTCA TCGACTGGTG GAGCAAATTC 
TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC GGCTCATACT TGGAGAAGGG CTTTGACACT 
CTAAAGGTCT ATGACACACA ACTGGAGAAC GTGGAGGCCT TTGAGGGCCT GTCTGACTTT 
TGTAACACCT TCAAGCTCTA CCGGGGCAAG ACTCAGGAGG AGACAGAGGA CCCATCTGTT 
ATTGGGGAGT TTAAGGGCCT CTTCAAAATC TATCCCCTCC CAGAAGACCC GGCTGTTCCC 
ATGCCCCCGA GACAATTCCA CCAGCTGGCT GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC 
ATCTATATCA TCCGAGCATT TGGCCTTCAG CCCAAGGACC CCAATGGGAA GTGTGATCCT 
TACATCAAGA TCTCCATAGG GAAGAAATCA GTGAGTGACC AGGATAACTA CATTCCCTGT 
ACTCTGGAGC CTGTGTTTGG AAAGATGTTC GAGCTGACCT GCACTCTGCC TCTGGAGAAG 
GACCTGAAGA TCACACTCTA TGACTATGAC CTCCTCTCCA AAGATGAAAA GATTGGGGAG 
ACAGTCATTG ACCTGGAGGA TAGGCTGCTG TCCAAGTTTG GGGCTCGCTG CGGACTCCCA 
CAGACGTACT GTGTCTCTGG ACCCAACCAG TGGAGGGACC AGCTCCGCCC CTCCCAGCTT 
CTCCACCTCT TCTGCCAGCA GCGCAGACTC AAGGCTCCTG TGTACCTCAC GGACCACGTG 
GTGTTCCAGG ATAAAGAATA CAGCATTGAA GAAATAGAGG CTGGCCGAGT CCCGAACCCA 
CACCTGGGCC CAGTGGAAGA GCGTCTGGCC TTGCATGTCC TCCAGCAGCA GGGCCTGGTC 
CCTGAGCATG TGGAGTCCCG GCCTCTCTAC AGCCCCCTGC AGCCAGACAT TGAACAGGGG 
AAGCTGCAGA TGTGGATCGA CCTATTTCCC AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC 
AACATCACCC CGCGGAGAGC CAGAAGGTTT TTCCTGCGTT GTATCATCTG GAACACAAAG 
GACGTGATCC TGGACGACCT CAGCATCACA GGGGAGAAGA TGAGTGATAT TTATGTGAAG 
GGTTGGATGG TTGGCTTTGA AGAGCACAAG CAAAAGACAG ATGTGCATTA CCGGTCCCTG 
GGAGGTGAAG GCAACTTTAA CTGGAGGTTC ATTTTCCCCT TTGACTACCT GCCAGCCGAG 
CAAGTCTGCA CCGTCTCTAA GAAGGATGCT TTCTGGAGGC TGGACAAGAC TGAGAGCAAA 
ATCCCAGCAC GAGTGGTATT TCAGATCTGG GACAATGACA AGTTCTCCTT TGACGACTTT 
CTGGGCTCCT TGCAGTTGGA TCTCAACCAC ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG 
TGCTCCTTGG ACCAGCTGGA TGACACTTTC CACCCGGAGT GGTTTGTGTC CCTTTTTGAA 
CAGAAAACAG TTAAGGGCTG GTGGCCCTGC GTAGCAGAAG AGGGCGAGAA GAAGATCCTG 
GCAGGCAAGC TGGAAATGAC CTTGGAGATC GTAGCAGAGA GTGAGCATGA GGAGCGGCCT 
GCTGGCCAGG GCCGGGATGA ACCCAACATG AACCCCAAGC TCGAGGACCC AAGGCGCCCT 
GACACCTCCT TCCTCTGGTT CACCTCTCCA TACAAGACTA TGAAGTTCAT CCTGTGGCGG 
CGGTTCCGAT GTGCCATTAT CCTCTTCATC ATTCTCTTCA TCCTGCTACT ATTCTTAGGC 
ATCTTCATCT ATGCTTTCCC GAACTACGCT GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432234.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432282.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	OCe19206
Clone ID Related Accession (Same CDS sequence)	XM_003432234.4 , XM_003432234.3
Accession Version	XM_003432234.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432234.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC 
ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG 
GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA 
GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC 
TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC 
TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC 
CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC 
ATCGATGACA AGGAGCCCCT CATCCCCATG CAGCTTGCAG ATGGTCTGTC GGGCTCTGCC 
CCCACTAACA TGGCATCTTC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC 
CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG 
GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC 
CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG 
ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC 
GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG 
GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC 
CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT 
GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA 
CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT 
TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC 
CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG 
AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA 
TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432282.1
CDS	23..6391
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432234.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432234.4

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC 
ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG 
GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA 
GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC 
TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC 
TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC 
CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC 
ATCGATGACA AGGAGCCCCT CATCCCCATG CAGCTTGCAG ATGGTCTGTC GGGCTCTGCC 
CCCACTAACA TGGCATCTTC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC 
CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG 
GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC 
CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG 
ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC 
GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG 
GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC 
CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT 
GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA 
CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT 
TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC 
CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG 
AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA 
TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432234.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432282.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA.

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CloneID	OCe19206
Clone ID Related Accession (Same CDS sequence)	XM_003432234.4 , XM_003432234.3
Accession Version	XM_003432234.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432234.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC 
ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG 
GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA 
GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC 
TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC 
TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC 
CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC 
ATCGATGACA AGGAGCCCCT CATCCCCATG CAGCTTGCAG ATGGTCTGTC GGGCTCTGCC 
CCCACTAACA TGGCATCTTC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC 
CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG 
GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC 
CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG 
ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC 
GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG 
GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC 
CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT 
GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA 
CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT 
TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC 
CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG 
AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA 
TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432282.1
CDS	51..6419
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432234.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432234.3

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC 
ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG 
GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA 
GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC 
TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC 
TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC 
CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC 
ATCGATGACA AGGAGCCCCT CATCCCCATG CAGCTTGCAG ATGGTCTGTC GGGCTCTGCC 
CCCACTAACA TGGCATCTTC TCCATCCAGT CCTCATGAGG AAGAGTTCAT CGACTGGTGG 
AGCAAATTCT TTGCCTCCAC AGGGGAGAGG GAAAAGTGCG GCTCATACTT GGAGAAGGGC 
TTTGACACTC TAAAGGTCTA TGACACACAA CTGGAGAACG TGGAGGCCTT TGAGGGCCTG 
TCTGACTTTT GTAACACCTT CAAGCTCTAC CGGGGCAAGA CTCAGGAGGA GACAGAGGAC 
CCATCTGTTA TTGGGGAGTT TAAGGGCCTC TTCAAAATCT ATCCCCTCCC AGAAGACCCG 
GCTGTTCCCA TGCCCCCGAG ACAATTCCAC CAGCTGGCTG CCCAAGGGCC TCAGGAGTGC 
CTGGTCCGCA TCTATATCAT CCGAGCATTT GGCCTTCAGC CCAAGGACCC CAATGGGAAG 
TGTGATCCTT ACATCAAGAT CTCCATAGGG AAGAAATCAG TGAGTGACCA GGATAACTAC 
ATTCCCTGTA CTCTGGAGCC TGTGTTTGGA AAGATGTTCG AGCTGACCTG CACTCTGCCT 
CTGGAGAAGG ACCTGAAGAT CACACTCTAT GACTATGACC TCCTCTCCAA AGATGAAAAG 
ATTGGGGAGA CAGTCATTGA CCTGGAGGAT AGGCTGCTGT CCAAGTTTGG GGCTCGCTGC 
GGACTCCCAC AGACGTACTG TGTCTCTGGA CCCAACCAGT GGAGGGACCA GCTCCGCCCC 
TCCCAGCTTC TCCACCTCTT CTGCCAGCAG CGCAGACTCA AGGCTCCTGT GTACCTCACG 
GACCACGTGG TGTTCCAGGA TAAAGAATAC AGCATTGAAG AAATAGAGGC TGGCCGAGTC 
CCGAACCCAC ACCTGGGCCC AGTGGAAGAG CGTCTGGCCT TGCATGTCCT CCAGCAGCAG 
GGCCTGGTCC CTGAGCATGT GGAGTCCCGG CCTCTCTACA GCCCCCTGCA GCCAGACATT 
GAACAGGGGA AGCTGCAGAT GTGGATCGAC CTATTTCCCA AGGCCCTGGG GCGTCCCGGA 
CCTCCCTTCA ACATCACCCC GCGGAGAGCC AGAAGGTTTT TCCTGCGTTG TATCATCTGG 
AACACAAAGG ACGTGATCCT GGACGACCTC AGCATCACAG GGGAGAAGAT GAGTGATATT 
TATGTGAAGG GTTGGATGGT TGGCTTTGAA GAGCACAAGC AAAAGACAGA TGTGCATTAC 
CGGTCCCTGG GAGGTGAAGG CAACTTTAAC TGGAGGTTCA TTTTCCCCTT TGACTACCTG 
CCAGCCGAGC AAGTCTGCAC CGTCTCTAAG AAGGATGCTT TCTGGAGGCT GGACAAGACT 
GAGAGCAAAA TCCCAGCACG AGTGGTATTT CAGATCTGGG ACAATGACAA GTTCTCCTTT 
GACGACTTTC TGGGCTCCTT GCAGTTGGAT CTCAACCACA TGCCCAAGCC TGCCAAGACA 
GCTGAGAAGT GCTCCTTGGA CCAGCTGGAT GACACTTTCC ACCCGGAGTG GTTTGTGTCC 
CTTTTTGAAC AGAAAACAGT TAAGGGCTGG TGGCCCTGCG TAGCAGAAGA GGGCGAGAAG 
AAGATCCTGG CAGGCAAGCT GGAAATGACC TTGGAGATCG TAGCAGAGAG TGAGCATGAG 
GAGCGGCCTG CTGGCCAGGG CCGGGATGAA CCCAACATGA ACCCCAAGCT CGAGGACCCA 
AGGCGCCCTG ACACCTCCTT CCTCTGGTTC ACCTCTCCAT ACAAGACTAT GAAGTTCATC 
CTGTGGCGGC GGTTCCGATG TGCCATTATC CTCTTCATCA TTCTCTTCAT CCTGCTACTA 
TTCTTAGGCA TCTTCATCTA TGCTTTCCCG AACTACGCTG CCATGAAGCT GGTGAAGCCC 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432229.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432277.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA.

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CloneID	OCe19207
Clone ID Related Accession (Same CDS sequence)	XM_003432229.3 , XM_003432229.4
Accession Version	XM_003432229.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432229.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG 
GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC 
CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG 
TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATGCAG CTTGCAGATG GTCTGTCGGG CTCTGCCCCC 
ACTAACATGG CATCTTCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA 
TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT 
GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA 
CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC 
CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT 
CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT 
GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG 
TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC 
TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432277.1
CDS	439..6804
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432229.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432229.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG 
GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC 
CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG 
TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATGCAG CTTGCAGATG GTCTGTCGGG CTCTGCCCCC 
ACTAACATGG CATCTTCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA 
TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT 
GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA 
CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC 
CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT 
CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT 
GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG 
TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC 
TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432229.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432277.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	OCe19207
Clone ID Related Accession (Same CDS sequence)	XM_003432229.3 , XM_003432229.4
Accession Version	XM_003432229.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6366bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432229.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG 
GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC 
CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG 
TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATGCAG CTTGCAGATG GTCTGTCGGG CTCTGCCCCC 
ACTAACATGG CATCTTCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA 
TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT 
GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA 
CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC 
CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT 
CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT 
GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG 
TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC 
TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432277.1
CDS	405..6770
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432229.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432229.4

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG 
GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC 
CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG 
TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATGCAG CTTGCAGATG GTCTGTCGGG CTCTGCCCCC 
ACTAACATGG CATCTTCTCC ATCCAGTCCT CATGAGGAAG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA 
TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT 
GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA 
CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC 
CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT 
CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT 
GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG 
TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC 
TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432230.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432278.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA.

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CloneID	OCe19208
Clone ID Related Accession (Same CDS sequence)	XM_003432230.3 , XM_003432230.4
Accession Version	XM_003432230.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432230.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA 
GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC 
AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG 
AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA 
GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT 
TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC 
ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG 
GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT 
GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC 
CTCATCCCCA TGCAGGAGGA AGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA 
GGGGAGAGGG AAAAGTGCGG CTCATACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT 
GACACACAAC TGGAGAACGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACAGAGGACC CATCTGTTAT TGGGGAGTTT 
AAGGGCCTCT TCAAAATCTA TCCCCTCCCA GAAGACCCGG CTGTTCCCAT GCCCCCGAGA 
CAATTCCACC AGCTGGCTGC CCAAGGGCCT CAGGAGTGCC TGGTCCGCAT CTATATCATC 
CGAGCATTTG GCCTTCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TTCCCTGTAC TCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACACTCTATG ACTATGACCT CCTCTCCAAA GATGAAAAGA TTGGGGAGAC AGTCATTGAC 
CTGGAGGATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GACTCCCACA GACGTACTGT 
GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTCTTC 
TGCCAGCAGC GCAGACTCAA GGCTCCTGTG TACCTCACGG ACCACGTGGT GTTCCAGGAT 
AAAGAATACA GCATTGAAGA AATAGAGGCT GGCCGAGTCC CGAACCCACA CCTGGGCCCA 
GTGGAAGAGC GTCTGGCCTT GCATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAGCATGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCAGACATTG AACAGGGGAA GCTGCAGATG 
TGGATCGACC TATTTCCCAA GGCCCTGGGG CGTCCCGGAC CTCCCTTCAA CATCACCCCG 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATCATCTGGA ACACAAAGGA CGTGATCCTG 
GACGACCTCA GCATCACAGG GGAGAAGATG AGTGATATTT ATGTGAAGGG TTGGATGGTT 
GGCTTTGAAG AGCACAAGCA AAAGACAGAT GTGCATTACC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CAGCCGAGCA AGTCTGCACC 
GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCCTTG 
CAGTTGGATC TCAACCACAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTT 
AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG GGCGAGAAGA AGATCCTGGC AGGCAAGCTG 
GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC 
CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCTCCATA CAAGACTATG AAGTTCATCC TGTGGCGGCG GTTCCGATGT 
GCCATTATCC TCTTCATCAT TCTCTTCATC CTGCTACTAT TCTTAGGCAT CTTCATCTAT 
GCTTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432278.1
CDS	51..6398
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432230.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432230.3

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA 
GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC 
AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG 
AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA 
GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT 
TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC 
ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG 
GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT 
GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC 
CTCATCCCCA TGCAGGAGGA AGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA 
GGGGAGAGGG AAAAGTGCGG CTCATACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT 
GACACACAAC TGGAGAACGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACAGAGGACC CATCTGTTAT TGGGGAGTTT 
AAGGGCCTCT TCAAAATCTA TCCCCTCCCA GAAGACCCGG CTGTTCCCAT GCCCCCGAGA 
CAATTCCACC AGCTGGCTGC CCAAGGGCCT CAGGAGTGCC TGGTCCGCAT CTATATCATC 
CGAGCATTTG GCCTTCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TTCCCTGTAC TCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACACTCTATG ACTATGACCT CCTCTCCAAA GATGAAAAGA TTGGGGAGAC AGTCATTGAC 
CTGGAGGATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GACTCCCACA GACGTACTGT 
GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTCTTC 
TGCCAGCAGC GCAGACTCAA GGCTCCTGTG TACCTCACGG ACCACGTGGT GTTCCAGGAT 
AAAGAATACA GCATTGAAGA AATAGAGGCT GGCCGAGTCC CGAACCCACA CCTGGGCCCA 
GTGGAAGAGC GTCTGGCCTT GCATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAGCATGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCAGACATTG AACAGGGGAA GCTGCAGATG 
TGGATCGACC TATTTCCCAA GGCCCTGGGG CGTCCCGGAC CTCCCTTCAA CATCACCCCG 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATCATCTGGA ACACAAAGGA CGTGATCCTG 
GACGACCTCA GCATCACAGG GGAGAAGATG AGTGATATTT ATGTGAAGGG TTGGATGGTT 
GGCTTTGAAG AGCACAAGCA AAAGACAGAT GTGCATTACC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CAGCCGAGCA AGTCTGCACC 
GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCCTTG 
CAGTTGGATC TCAACCACAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTT 
AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG GGCGAGAAGA AGATCCTGGC AGGCAAGCTG 
GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC 
CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCTCCATA CAAGACTATG AAGTTCATCC TGTGGCGGCG GTTCCGATGT 
GCCATTATCC TCTTCATCAT TCTCTTCATC CTGCTACTAT TCTTAGGCAT CTTCATCTAT 
GCTTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432230.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432278.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	OCe19208
Clone ID Related Accession (Same CDS sequence)	XM_003432230.3 , XM_003432230.4
Accession Version	XM_003432230.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432230.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA 
GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC 
AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG 
AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA 
GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT 
TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC 
ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG 
GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT 
GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC 
CTCATCCCCA TGCAGGAGGA AGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA 
GGGGAGAGGG AAAAGTGCGG CTCATACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT 
GACACACAAC TGGAGAACGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACAGAGGACC CATCTGTTAT TGGGGAGTTT 
AAGGGCCTCT TCAAAATCTA TCCCCTCCCA GAAGACCCGG CTGTTCCCAT GCCCCCGAGA 
CAATTCCACC AGCTGGCTGC CCAAGGGCCT CAGGAGTGCC TGGTCCGCAT CTATATCATC 
CGAGCATTTG GCCTTCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TTCCCTGTAC TCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACACTCTATG ACTATGACCT CCTCTCCAAA GATGAAAAGA TTGGGGAGAC AGTCATTGAC 
CTGGAGGATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GACTCCCACA GACGTACTGT 
GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTCTTC 
TGCCAGCAGC GCAGACTCAA GGCTCCTGTG TACCTCACGG ACCACGTGGT GTTCCAGGAT 
AAAGAATACA GCATTGAAGA AATAGAGGCT GGCCGAGTCC CGAACCCACA CCTGGGCCCA 
GTGGAAGAGC GTCTGGCCTT GCATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAGCATGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCAGACATTG AACAGGGGAA GCTGCAGATG 
TGGATCGACC TATTTCCCAA GGCCCTGGGG CGTCCCGGAC CTCCCTTCAA CATCACCCCG 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATCATCTGGA ACACAAAGGA CGTGATCCTG 
GACGACCTCA GCATCACAGG GGAGAAGATG AGTGATATTT ATGTGAAGGG TTGGATGGTT 
GGCTTTGAAG AGCACAAGCA AAAGACAGAT GTGCATTACC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CAGCCGAGCA AGTCTGCACC 
GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCCTTG 
CAGTTGGATC TCAACCACAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTT 
AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG GGCGAGAAGA AGATCCTGGC AGGCAAGCTG 
GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC 
CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCTCCATA CAAGACTATG AAGTTCATCC TGTGGCGGCG GTTCCGATGT 
GCCATTATCC TCTTCATCAT TCTCTTCATC CTGCTACTAT TCTTAGGCAT CTTCATCTAT 
GCTTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432278.1
CDS	23..6370
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432230.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432230.4

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGAGGAGCCT GCAGGTGTGG TCAAGCCTTC TCCAGCCACG 
GACCTGGATG ACCATCTGGG CTTCCTTCCC ACCTTCGGGC CCTGCTTCGT CAATCTCTAT 
GGCAGCCCCA GGGAATTCAC TGGCTTCCCG GACCCCTACG CGGAACTCAA CATGGGCAAG 
GGGGAAGGCG TGGCCTACCG AGGTCGGCTT CTGCTCTCCC TGGAGACCAA GCTGGTGGAG 
CACAGCGGCG AGCAAAGGGT GGAGGACCTC CCTGCTGATG ACATCCTTCG GGTGGAGAAA 
TACCTCCGGA GGCGCAAGTA CTCTCTCTTT GCCGCCTTCT ACTCGGCCAC CATGCTCCAG 
GATGTCGACG ATGCCATCCA GTTTGAGGTC AGCATTGGGA ACTATGGCAA CAAGTTCGAC 
ACGACCTGTC TGCCACTGGC CTCCACCACC CAGTACAGCC GGGCAGTCTT TGATGGGTGC 
CACTACTACT ACTTGCCCTG GGGCAATGTG AAGCCTGTGG TGGTGCTGTC ATCCTACTGG 
GAAGACATCA GCCACAGAGT CGAGGCTCAG AACCAGCTGC TCAGGATCGC TGACCATCTG 
GAAGCTGGCC TGGAGCAAGT CCACTTGGCT TTAAAGGCGC AGTTCAACAC AGAGGATGTG 
GACTCCCTGT TGGCCCAGCT GATGGATGAG CTCATCTCAG ACTGCAGCCA GCCCCTGGGC 
AATGTCCAAG AGATACCCTC TGCCACCCAC CTGGACCGGC ACCTGTACAG GCTGCGCACC 
CGTCACCTAG GCCAGATTAC TGAGGCGGCC CTGGCTCTGA AACTCGGGCA TGGCAAGCTC 
CCTGCCGCCC TGGAGCAGGC TGAAGACTGG CTCCTGCGTC TCCGTGCCCT GGCTGATGAG 
CCCCAGAACA GCCTGCCCGA CATCATCATC TGGATGCTGC AGGGAAACAA GCGAGTGGCG 
TACCAGCGGG TGCCTGCGCA CGAGGTCCTC TTCTCCCGGC GGGGCGCCAG CTACTGTGGC 
AAGAACTGTG GGAAGCTGCA GACGATATTT CTGAAATATC CCATGGAGGG GGTGCCCGGA 
GCCCGGATGC CAGTACAAAT ACGGGTGAAA CTCTGGTTTG GGCTTTCTGT GGATGAGAAG 
GAGTTCAACC AGTTTGCTGA GGGAAAGCTG TCCGTCTTTG CTGAAACTTA TGAGAACCAG 
ACCAAACTGG CCCTGGTTGG GAACTGGGGC ACAACAGGCC TCACCTACCC CAAGTTTTCT 
GATGTCATGG GCAAGATCAA GCTACCCAAG GATAGCTTCC GCCCCTCAGC TGGCTGGACC 
TGGGCCGGAG ACTGGTTCGT GTGTCCAGAG AAGACCCTAC TCCATGACAT GGACGCGGGC 
CACCTGAGTT TTGTGGAGGA GGTGTTTGAG AACCAGACCC GGCTCCCTGG AGGCCAGTGG 
ATCTACATGA GTGACAACTA CACAGATGTG AATGGGGATA AGGTGCTTCC TAAGGATGAC 
ATTGAGTGCC CACTGGGCTG GAAGTGGGAA GATGAAGAGT GGTCCACGGA CCTCAATCGG 
GCCGTCGATG AGCAAGGCTG GGAGTACAGT ATCACCATCC CCCCGGATCG GAAGCCAAAG 
CACTGGGTTC CTGCTGAGAA GATGTATTAC ACGCACCGTC GGCGGCGCTG GGTCCGCCTG 
CGCAGAAGGG ACCTCAGCCA GATGGAGGCA TTGAAGAGGC ACAGACAGGC AGAGGCAGAG 
GGTGAGGGCT GGGAGTACGC CTCGCTCTTG GGCTGGAAGT TCCACCTGGA GTACCGCAAG 
ACTGATGCTT TCCGCCGCCG CCGCTGGCGC CGCCGCATGG AACCGCTGGA GAAGACGGGG 
CCTGCAGCCG TGTTTGCCCT TGAGGGGGCT CTGGGTGGTG TGGTAGATGA CAGGAGTGAA 
GATTCCGTGT CCGTGTCCAC CTTGAGCTTT GGAGTGAACA GACCCACGAT TTCCTGCATC 
TTTGATTATG GGAACCGCTA CCACCTACGC TGCTACATGT ACCAGGCTCG GGACCTGCCC 
TCGATGGACA AGGACTCTTT TTCTGATCCC TACGCCATCG TCTCCTTCCT GCACCAGAGC 
CAGAAGACAG TGGTGGCGAA GAACACTCTG AACCCCACCT GGGACCAGAC GCTTATCTTC 
TATGAGATTG AGATCTTTGG TGAGCCAGCC AGTATCGCTG AGCAGCCGCC CAGCATTGTG 
GTGGAGCTGT ATGACCACGA CACCTACGGT GCAGATGAGT TTATGGGACG CTGCATTTGT 
CAGCCCAGCC TGGAGCGGAC ACCCCGGCTG GCCTGGTTCC CACTGATTAG GGGCAGCCAG 
TCGGCGGGGG AACTGCTGGC CTCTTTTGAG CTCATCCAGA GAGAAAAGCC GGCCATTCAC 
CATATTCCTG GTTTTGAGGT ACAGGATATG TCAGGAATCC TGGAAGAGTC AGAGGACATA 
GACCTGCCCT ACCCGCCACC TCAGAGGGAG GCCAACATCT TCATGGTCCC CCAGAACATC 
AAGCCTGTTC TCCAGCGTAC TGCCATAGAG ATCCTGGCGT GGGGCCTGCG GAACATGAAG 
AGCTACCAGC TAGCCAGCAT CTCCTCCCCT AGCCTTGTGG TAGAGTGCGG GGGCCAGACA 
GTGCAGTCCT GTGTCATCAA GAACCTCCGG AAGAACCCCA ACTTCGACAT CTGTACCCTT 
TTCATGGAAG TGATGCTGCC CAAGGAGGAG GTCTACTGTC CCTCCATCGT GATCAAGGTC 
ATTGATAACC GCCAGTTTGG CCGCCGGCCT GTGGTGGGCC AGTGTACCAT CCGTTCCCTG 
GAGAGCTTCA TGTGTGACCT GTACTCCGAG GAGAATCCGT CCCCACAGGG CGGCCCAGAT 
GATGTGAGTT TACTCAGTCC TGGGGAAGAC GTGCTCATTG ACATCGATGA CAAGGAGCCC 
CTCATCCCCA TGCAGGAGGA AGAGTTCATC GACTGGTGGA GCAAATTCTT TGCCTCCACA 
GGGGAGAGGG AAAAGTGCGG CTCATACTTG GAGAAGGGCT TTGACACTCT AAAGGTCTAT 
GACACACAAC TGGAGAACGT GGAGGCCTTT GAGGGCCTGT CTGACTTTTG TAACACCTTC 
AAGCTCTACC GGGGCAAGAC TCAGGAGGAG ACAGAGGACC CATCTGTTAT TGGGGAGTTT 
AAGGGCCTCT TCAAAATCTA TCCCCTCCCA GAAGACCCGG CTGTTCCCAT GCCCCCGAGA 
CAATTCCACC AGCTGGCTGC CCAAGGGCCT CAGGAGTGCC TGGTCCGCAT CTATATCATC 
CGAGCATTTG GCCTTCAGCC CAAGGACCCC AATGGGAAGT GTGATCCTTA CATCAAGATC 
TCCATAGGGA AGAAATCAGT GAGTGACCAG GATAACTACA TTCCCTGTAC TCTGGAGCCT 
GTGTTTGGAA AGATGTTCGA GCTGACCTGC ACTCTGCCTC TGGAGAAGGA CCTGAAGATC 
ACACTCTATG ACTATGACCT CCTCTCCAAA GATGAAAAGA TTGGGGAGAC AGTCATTGAC 
CTGGAGGATA GGCTGCTGTC CAAGTTTGGG GCTCGCTGCG GACTCCCACA GACGTACTGT 
GTCTCTGGAC CCAACCAGTG GAGGGACCAG CTCCGCCCCT CCCAGCTTCT CCACCTCTTC 
TGCCAGCAGC GCAGACTCAA GGCTCCTGTG TACCTCACGG ACCACGTGGT GTTCCAGGAT 
AAAGAATACA GCATTGAAGA AATAGAGGCT GGCCGAGTCC CGAACCCACA CCTGGGCCCA 
GTGGAAGAGC GTCTGGCCTT GCATGTCCTC CAGCAGCAGG GCCTGGTCCC TGAGCATGTG 
GAGTCCCGGC CTCTCTACAG CCCCCTGCAG CCAGACATTG AACAGGGGAA GCTGCAGATG 
TGGATCGACC TATTTCCCAA GGCCCTGGGG CGTCCCGGAC CTCCCTTCAA CATCACCCCG 
CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT ATCATCTGGA ACACAAAGGA CGTGATCCTG 
GACGACCTCA GCATCACAGG GGAGAAGATG AGTGATATTT ATGTGAAGGG TTGGATGGTT 
GGCTTTGAAG AGCACAAGCA AAAGACAGAT GTGCATTACC GGTCCCTGGG AGGTGAAGGC 
AACTTTAACT GGAGGTTCAT TTTCCCCTTT GACTACCTGC CAGCCGAGCA AGTCTGCACC 
GTCTCTAAGA AGGATGCTTT CTGGAGGCTG GACAAGACTG AGAGCAAAAT CCCAGCACGA 
GTGGTATTTC AGATCTGGGA CAATGACAAG TTCTCCTTTG ACGACTTTCT GGGCTCCTTG 
CAGTTGGATC TCAACCACAT GCCCAAGCCT GCCAAGACAG CTGAGAAGTG CTCCTTGGAC 
CAGCTGGATG ACACTTTCCA CCCGGAGTGG TTTGTGTCCC TTTTTGAACA GAAAACAGTT 
AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG GGCGAGAAGA AGATCCTGGC AGGCAAGCTG 
GAAATGACCT TGGAGATCGT AGCAGAGAGT GAGCATGAGG AGCGGCCTGC TGGCCAGGGC 
CGGGATGAAC CCAACATGAA CCCCAAGCTC GAGGACCCAA GGCGCCCTGA CACCTCCTTC 
CTCTGGTTCA CCTCTCCATA CAAGACTATG AAGTTCATCC TGTGGCGGCG GTTCCGATGT 
GCCATTATCC TCTTCATCAT TCTCTTCATC CTGCTACTAT TCTTAGGCAT CTTCATCTAT 
GCTTTCCCGA ACTACGCTGC CATGAAGCTG GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432225.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432273.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	OCe19209
Clone ID Related Accession (Same CDS sequence)	XM_003432225.4 , XM_003432225.3
Accession Version	XM_003432225.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432225.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC 
CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC 
AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC 
CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT 
GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG 
ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA 
GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA 
GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC 
TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT 
GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT 
CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC 
CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC 
CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC 
AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG 
GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC 
CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT 
GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC 
TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT 
GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT 
GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT 
TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT 
GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG 
CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG 
GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGTCAGA GGACATAGAC 
CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG 
CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC 
TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC 
ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATGC AGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG 
GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC 
ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG 
GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA 
TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA 
GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA 
CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG 
GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC 
TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC 
CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA 
GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG 
GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG 
TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG 
ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG 
AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC 
GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC 
TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC 
TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC 
TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG 
GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG 
TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG 
GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA 
ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG 
GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC 
TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC 
ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT 
TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432273.1
CDS	405..6749
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432225.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432225.4

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC 
CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC 
AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC 
CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT 
GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG 
ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA 
GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA 
GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC 
TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT 
GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT 
CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC 
CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC 
CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC 
AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG 
GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC 
CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT 
GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC 
TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT 
GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT 
GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT 
TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT 
GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG 
CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG 
GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGTCAGA GGACATAGAC 
CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG 
CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC 
TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC 
ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATGC AGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG 
GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC 
ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG 
GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA 
TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA 
GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA 
CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG 
GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC 
TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC 
CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA 
GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG 
GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG 
TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG 
ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG 
AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC 
GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC 
TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC 
TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC 
TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG 
GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG 
TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG 
GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA 
ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG 
GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC 
TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC 
ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT 
TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432225.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432273.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA.

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CloneID	OCe19209
Clone ID Related Accession (Same CDS sequence)	XM_003432225.4 , XM_003432225.3
Accession Version	XM_003432225.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6345bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432225.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC 
CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC 
AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC 
CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT 
GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG 
ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA 
GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA 
GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC 
TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT 
GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT 
CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC 
CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC 
CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC 
AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG 
GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC 
CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT 
GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC 
TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT 
GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT 
GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT 
TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT 
GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG 
CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG 
GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGTCAGA GGACATAGAC 
CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG 
CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC 
TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC 
ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATGC AGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG 
GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC 
ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG 
GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA 
TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA 
GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA 
CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG 
GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC 
TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC 
CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA 
GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG 
GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG 
TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG 
ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG 
AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC 
GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC 
TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC 
TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC 
TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG 
GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG 
TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG 
GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA 
ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG 
GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC 
TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC 
ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT 
TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432273.1
CDS	439..6783
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432225.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432225.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGA GGAGCCTGCA GGTGTGGTCA AGCCTTCTCC AGCCACGGAC 
CTGGATGACC ATCTGGGCTT CCTTCCCACC TTCGGGCCCT GCTTCGTCAA TCTCTATGGC 
AGCCCCAGGG AATTCACTGG CTTCCCGGAC CCCTACGCGG AACTCAACAT GGGCAAGGGG 
GAAGGCGTGG CCTACCGAGG TCGGCTTCTG CTCTCCCTGG AGACCAAGCT GGTGGAGCAC 
AGCGGCGAGC AAAGGGTGGA GGACCTCCCT GCTGATGACA TCCTTCGGGT GGAGAAATAC 
CTCCGGAGGC GCAAGTACTC TCTCTTTGCC GCCTTCTACT CGGCCACCAT GCTCCAGGAT 
GTCGACGATG CCATCCAGTT TGAGGTCAGC ATTGGGAACT ATGGCAACAA GTTCGACACG 
ACCTGTCTGC CACTGGCCTC CACCACCCAG TACAGCCGGG CAGTCTTTGA TGGGTGCCAC 
TACTACTACT TGCCCTGGGG CAATGTGAAG CCTGTGGTGG TGCTGTCATC CTACTGGGAA 
GACATCAGCC ACAGAGTCGA GGCTCAGAAC CAGCTGCTCA GGATCGCTGA CCATCTGGAA 
GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA AAGGCGCAGT TCAACACAGA GGATGTGGAC 
TCCCTGTTGG CCCAGCTGAT GGATGAGCTC ATCTCAGACT GCAGCCAGCC CCTGGGCAAT 
GTCCAAGAGA TACCCTCTGC CACCCACCTG GACCGGCACC TGTACAGGCT GCGCACCCGT 
CACCTAGGCC AGATTACTGA GGCGGCCCTG GCTCTGAAAC TCGGGCATGG CAAGCTCCCT 
GCCGCCCTGG AGCAGGCTGA AGACTGGCTC CTGCGTCTCC GTGCCCTGGC TGATGAGCCC 
CAGAACAGCC TGCCCGACAT CATCATCTGG ATGCTGCAGG GAAACAAGCG AGTGGCGTAC 
CAGCGGGTGC CTGCGCACGA GGTCCTCTTC TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG 
AACTGTGGGA AGCTGCAGAC GATATTTCTG AAATATCCCA TGGAGGGGGT GCCCGGAGCC 
CGGATGCCAG TACAAATACG GGTGAAACTC TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG 
TTCAACCAGT TTGCTGAGGG AAAGCTGTCC GTCTTTGCTG AAACTTATGA GAACCAGACC 
AAACTGGCCC TGGTTGGGAA CTGGGGCACA ACAGGCCTCA CCTACCCCAA GTTTTCTGAT 
GTCATGGGCA AGATCAAGCT ACCCAAGGAT AGCTTCCGCC CCTCAGCTGG CTGGACCTGG 
GCCGGAGACT GGTTCGTGTG TCCAGAGAAG ACCCTACTCC ATGACATGGA CGCGGGCCAC 
CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC CAGACCCGGC TCCCTGGAGG CCAGTGGATC 
TACATGAGTG ACAACTACAC AGATGTGAAT GGGGATAAGG TGCTTCCTAA GGATGACATT 
GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT GAAGAGTGGT CCACGGACCT CAATCGGGCC 
GTCGATGAGC AAGGCTGGGA GTACAGTATC ACCATCCCCC CGGATCGGAA GCCAAAGCAC 
TGGGTTCCTG CTGAGAAGAT GTATTACACG CACCGTCGGC GGCGCTGGGT CCGCCTGCGC 
AGAAGGGACC TCAGCCAGAT GGAGGCATTG AAGAGGCACA GACAGGCAGA GGCAGAGGGT 
GAGGGCTGGG AGTACGCCTC GCTCTTGGGC TGGAAGTTCC ACCTGGAGTA CCGCAAGACT 
GATGCTTTCC GCCGCCGCCG CTGGCGCCGC CGCATGGAAC CGCTGGAGAA GACGGGGCCT 
GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG GGTGGTGTGG TAGATGACAG GAGTGAAGAT 
TCCGTGTCCG TGTCCACCTT GAGCTTTGGA GTGAACAGAC CCACGATTTC CTGCATCTTT 
GATTATGGGA ACCGCTACCA CCTACGCTGC TACATGTACC AGGCTCGGGA CCTGCCCTCG 
ATGGACAAGG ACTCTTTTTC TGATCCCTAC GCCATCGTCT CCTTCCTGCA CCAGAGCCAG 
AAGACAGTGG TGGCGAAGAA CACTCTGAAC CCCACCTGGG ACCAGACGCT TATCTTCTAT 
GAGATTGAGA TCTTTGGTGA GCCAGCCAGT ATCGCTGAGC AGCCGCCCAG CATTGTGGTG 
GAGCTGTATG ACCACGACAC CTACGGTGCA GATGAGTTTA TGGGACGCTG CATTTGTCAG 
CCCAGCCTGG AGCGGACACC CCGGCTGGCC TGGTTCCCAC TGATTAGGGG CAGCCAGTCG 
GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC ATCCAGAGAG AAAAGCCGGC CATTCACCAT 
ATTCCTGGTT TTGAGGTACA GGATATGTCA GGAATCCTGG AAGAGTCAGA GGACATAGAC 
CTGCCCTACC CGCCACCTCA GAGGGAGGCC AACATCTTCA TGGTCCCCCA GAACATCAAG 
CCTGTTCTCC AGCGTACTGC CATAGAGATC CTGGCGTGGG GCCTGCGGAA CATGAAGAGC 
TACCAGCTAG CCAGCATCTC CTCCCCTAGC CTTGTGGTAG AGTGCGGGGG CCAGACAGTG 
CAGTCCTGTG TCATCAAGAA CCTCCGGAAG AACCCCAACT TCGACATCTG TACCCTTTTC 
ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC TACTGTCCCT CCATCGTGAT CAAGGTCATT 
GATAACCGCC AGTTTGGCCG CCGGCCTGTG GTGGGCCAGT GTACCATCCG TTCCCTGGAG 
AGCTTCATGT GTGACCTGTA CTCCGAGGAG AATCCGTCCC CACAGGGCGG CCCAGATGAT 
GTGAGTTTAC TCAGTCCTGG GGAAGACGTG CTCATTGACA TCGATGACAA GGAGCCCCTC 
ATCCCCATGC AGGAGGAAGA GTTCATCGAC TGGTGGAGCA AATTCTTTGC CTCCACAGGG 
GAGAGGGAAA AGTGCGGCTC ATACTTGGAG AAGGGCTTTG ACACTCTAAA GGTCTATGAC 
ACACAACTGG AGAACGTGGA GGCCTTTGAG GGCCTGTCTG ACTTTTGTAA CACCTTCAAG 
CTCTACCGGG GCAAGACTCA GGAGGAGACA GAGGACCCAT CTGTTATTGG GGAGTTTAAG 
GGCCTCTTCA AAATCTATCC CCTCCCAGAA GACCCGGCTG TTCCCATGCC CCCGAGACAA 
TTCCACCAGC TGGCTGCCCA AGGGCCTCAG GAGTGCCTGG TCCGCATCTA TATCATCCGA 
GCATTTGGCC TTCAGCCCAA GGACCCCAAT GGGAAGTGTG ATCCTTACAT CAAGATCTCC 
ATAGGGAAGA AATCAGTGAG TGACCAGGAT AACTACATTC CCTGTACTCT GGAGCCTGTG 
TTTGGAAAGA TGTTCGAGCT GACCTGCACT CTGCCTCTGG AGAAGGACCT GAAGATCACA 
CTCTATGACT ATGACCTCCT CTCCAAAGAT GAAAAGATTG GGGAGACAGT CATTGACCTG 
GAGGATAGGC TGCTGTCCAA GTTTGGGGCT CGCTGCGGAC TCCCACAGAC GTACTGTGTC 
TCTGGACCCA ACCAGTGGAG GGACCAGCTC CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC 
CAGCAGCGCA GACTCAAGGC TCCTGTGTAC CTCACGGACC ACGTGGTGTT CCAGGATAAA 
GAATACAGCA TTGAAGAAAT AGAGGCTGGC CGAGTCCCGA ACCCACACCT GGGCCCAGTG 
GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG CAGCAGGGCC TGGTCCCTGA GCATGTGGAG 
TCCCGGCCTC TCTACAGCCC CCTGCAGCCA GACATTGAAC AGGGGAAGCT GCAGATGTGG 
ATCGACCTAT TTCCCAAGGC CCTGGGGCGT CCCGGACCTC CCTTCAACAT CACCCCGCGG 
AGAGCCAGAA GGTTTTTCCT GCGTTGTATC ATCTGGAACA CAAAGGACGT GATCCTGGAC 
GACCTCAGCA TCACAGGGGA GAAGATGAGT GATATTTATG TGAAGGGTTG GATGGTTGGC 
TTTGAAGAGC ACAAGCAAAA GACAGATGTG CATTACCGGT CCCTGGGAGG TGAAGGCAAC 
TTTAACTGGA GGTTCATTTT CCCCTTTGAC TACCTGCCAG CCGAGCAAGT CTGCACCGTC 
TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC AAGACTGAGA GCAAAATCCC AGCACGAGTG 
GTATTTCAGA TCTGGGACAA TGACAAGTTC TCCTTTGACG ACTTTCTGGG CTCCTTGCAG 
TTGGATCTCA ACCACATGCC CAAGCCTGCC AAGACAGCTG AGAAGTGCTC CTTGGACCAG 
CTGGATGACA CTTTCCACCC GGAGTGGTTT GTGTCCCTTT TTGAACAGAA AACAGTTAAG 
GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA 
ATGACCTTGG AGATCGTAGC AGAGAGTGAG CATGAGGAGC GGCCTGCTGG CCAGGGCCGG 
GATGAACCCA ACATGAACCC CAAGCTCGAG GACCCAAGGC GCCCTGACAC CTCCTTCCTC 
TGGTTCACCT CTCCATACAA GACTATGAAG TTCATCCTGT GGCGGCGGTT CCGATGTGCC 
ATTATCCTCT TCATCATTCT CTTCATCCTG CTACTATTCT TAGGCATCTT CATCTATGCT 
TTCCCGAACT ACGCTGCCAT GAAGCTGGTG AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432226.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432274.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	OCe19210
Clone ID Related Accession (Same CDS sequence)	XM_003432226.4 , XM_003432226.3
Accession Version	XM_003432226.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432226.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC 
AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC 
CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC 
CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG 
AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC 
TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG 
AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG 
CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGCTTGCAGA TGGTCTGTCG 
GGCTCTGCCC CCACTAACAT GGCATCTTCT CCATCCAGTC CTCATGAGGA AGAGTTCATC 
GACTGGTGGA GCAAATTCTT TGCCTCCACA GGGGAGAGGG AAAAGTGCGG CTCATACTTG 
GAGAAGGGCT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAACGT GGAGGCCTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG 
ACAGAGGACC CATCTGTTAT TGGGGAGTTT AAGGGCCTCT TCAAAATCTA TCCCCTCCCA 
GAAGACCCGG CTGTTCCCAT GCCCCCGAGA CAATTCCACC AGCTGGCTGC CCAAGGGCCT 
CAGGAGTGCC TGGTCCGCAT CTATATCATC CGAGCATTTG GCCTTCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG 
GATAACTACA TTCCCTGTAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACACTCTATG ACTATGACCT CCTCTCCAAA 
GATGAAAAGA TTGGGGAGAC AGTCATTGAC CTGGAGGATA GGCTGCTGTC CAAGTTTGGG 
GCTCGCTGCG GACTCCCACA GACGTACTGT GTCTCTGGAC CCAACCAGTG GAGGGACCAG 
CTCCGCCCCT CCCAGCTTCT CCACCTCTTC TGCCAGCAGC GCAGACTCAA GGCTCCTGTG 
TACCTCACGG ACCACGTGGT GTTCCAGGAT AAAGAATACA GCATTGAAGA AATAGAGGCT 
GGCCGAGTCC CGAACCCACA CCTGGGCCCA GTGGAAGAGC GTCTGGCCTT GCATGTCCTC 
CAGCAGCAGG GCCTGGTCCC TGAGCATGTG GAGTCCCGGC CTCTCTACAG CCCCCTGCAG 
CCAGACATTG AACAGGGGAA GCTGCAGATG TGGATCGACC TATTTCCCAA GGCCCTGGGG 
CGTCCCGGAC CTCCCTTCAA CATCACCCCG CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT 
ATCATCTGGA ACACAAAGGA CGTGATCCTG GACGACCTCA GCATCACAGG GGAGAAGATG 
AGTGATATTT ATGTGAAGGG TTGGATGGTT GGCTTTGAAG AGCACAAGCA AAAGACAGAT 
GTGCATTACC GGTCCCTGGG AGGTGAAGGC AACTTTAACT GGAGGTTCAT TTTCCCCTTT 
GACTACCTGC CAGCCGAGCA AGTCTGCACC GTCTCTAAGA AGGATGCTTT CTGGAGGCTG 
GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTATTTC AGATCTGGGA CAATGACAAG 
TTCTCCTTTG ACGACTTTCT GGGCTCCTTG CAGTTGGATC TCAACCACAT GCCCAAGCCT 
GCCAAGACAG CTGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCGGAGTGG 
TTTGTGTCCC TTTTTGAACA GAAAACAGTT AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG 
GGCGAGAAGA AGATCCTGGC AGGCAAGCTG GAAATGACCT TGGAGATCGT AGCAGAGAGT 
GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAAC CCAACATGAA CCCCAAGCTC 
GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCTCCATA CAAGACTATG 
AAGTTCATCC TGTGGCGGCG GTTCCGATGT GCCATTATCC TCTTCATCAT TCTCTTCATC 
CTGCTACTAT TCTTAGGCAT CTTCATCTAT GCTTTCCCGA ACTACGCTGC CATGAAGCTG 
GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432274.1
CDS	23..6340
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432226.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432226.4

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC 
AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC 
CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC 
CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG 
AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC 
TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG 
AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG 
CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGCTTGCAGA TGGTCTGTCG 
GGCTCTGCCC CCACTAACAT GGCATCTTCT CCATCCAGTC CTCATGAGGA AGAGTTCATC 
GACTGGTGGA GCAAATTCTT TGCCTCCACA GGGGAGAGGG AAAAGTGCGG CTCATACTTG 
GAGAAGGGCT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAACGT GGAGGCCTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG 
ACAGAGGACC CATCTGTTAT TGGGGAGTTT AAGGGCCTCT TCAAAATCTA TCCCCTCCCA 
GAAGACCCGG CTGTTCCCAT GCCCCCGAGA CAATTCCACC AGCTGGCTGC CCAAGGGCCT 
CAGGAGTGCC TGGTCCGCAT CTATATCATC CGAGCATTTG GCCTTCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG 
GATAACTACA TTCCCTGTAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACACTCTATG ACTATGACCT CCTCTCCAAA 
GATGAAAAGA TTGGGGAGAC AGTCATTGAC CTGGAGGATA GGCTGCTGTC CAAGTTTGGG 
GCTCGCTGCG GACTCCCACA GACGTACTGT GTCTCTGGAC CCAACCAGTG GAGGGACCAG 
CTCCGCCCCT CCCAGCTTCT CCACCTCTTC TGCCAGCAGC GCAGACTCAA GGCTCCTGTG 
TACCTCACGG ACCACGTGGT GTTCCAGGAT AAAGAATACA GCATTGAAGA AATAGAGGCT 
GGCCGAGTCC CGAACCCACA CCTGGGCCCA GTGGAAGAGC GTCTGGCCTT GCATGTCCTC 
CAGCAGCAGG GCCTGGTCCC TGAGCATGTG GAGTCCCGGC CTCTCTACAG CCCCCTGCAG 
CCAGACATTG AACAGGGGAA GCTGCAGATG TGGATCGACC TATTTCCCAA GGCCCTGGGG 
CGTCCCGGAC CTCCCTTCAA CATCACCCCG CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT 
ATCATCTGGA ACACAAAGGA CGTGATCCTG GACGACCTCA GCATCACAGG GGAGAAGATG 
AGTGATATTT ATGTGAAGGG TTGGATGGTT GGCTTTGAAG AGCACAAGCA AAAGACAGAT 
GTGCATTACC GGTCCCTGGG AGGTGAAGGC AACTTTAACT GGAGGTTCAT TTTCCCCTTT 
GACTACCTGC CAGCCGAGCA AGTCTGCACC GTCTCTAAGA AGGATGCTTT CTGGAGGCTG 
GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTATTTC AGATCTGGGA CAATGACAAG 
TTCTCCTTTG ACGACTTTCT GGGCTCCTTG CAGTTGGATC TCAACCACAT GCCCAAGCCT 
GCCAAGACAG CTGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCGGAGTGG 
TTTGTGTCCC TTTTTGAACA GAAAACAGTT AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG 
GGCGAGAAGA AGATCCTGGC AGGCAAGCTG GAAATGACCT TGGAGATCGT AGCAGAGAGT 
GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAAC CCAACATGAA CCCCAAGCTC 
GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCTCCATA CAAGACTATG 
AAGTTCATCC TGTGGCGGCG GTTCCGATGT GCCATTATCC TCTTCATCAT TCTCTTCATC 
CTGCTACTAT TCTTAGGCAT CTTCATCTAT GCTTTCCCGA ACTACGCTGC CATGAAGCTG 
GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432226.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432274.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA.

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CloneID	OCe19210
Clone ID Related Accession (Same CDS sequence)	XM_003432226.4 , XM_003432226.3
Accession Version	XM_003432226.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432226.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC 
AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC 
CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC 
CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG 
AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC 
TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG 
AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG 
CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGCTTGCAGA TGGTCTGTCG 
GGCTCTGCCC CCACTAACAT GGCATCTTCT CCATCCAGTC CTCATGAGGA AGAGTTCATC 
GACTGGTGGA GCAAATTCTT TGCCTCCACA GGGGAGAGGG AAAAGTGCGG CTCATACTTG 
GAGAAGGGCT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAACGT GGAGGCCTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG 
ACAGAGGACC CATCTGTTAT TGGGGAGTTT AAGGGCCTCT TCAAAATCTA TCCCCTCCCA 
GAAGACCCGG CTGTTCCCAT GCCCCCGAGA CAATTCCACC AGCTGGCTGC CCAAGGGCCT 
CAGGAGTGCC TGGTCCGCAT CTATATCATC CGAGCATTTG GCCTTCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG 
GATAACTACA TTCCCTGTAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACACTCTATG ACTATGACCT CCTCTCCAAA 
GATGAAAAGA TTGGGGAGAC AGTCATTGAC CTGGAGGATA GGCTGCTGTC CAAGTTTGGG 
GCTCGCTGCG GACTCCCACA GACGTACTGT GTCTCTGGAC CCAACCAGTG GAGGGACCAG 
CTCCGCCCCT CCCAGCTTCT CCACCTCTTC TGCCAGCAGC GCAGACTCAA GGCTCCTGTG 
TACCTCACGG ACCACGTGGT GTTCCAGGAT AAAGAATACA GCATTGAAGA AATAGAGGCT 
GGCCGAGTCC CGAACCCACA CCTGGGCCCA GTGGAAGAGC GTCTGGCCTT GCATGTCCTC 
CAGCAGCAGG GCCTGGTCCC TGAGCATGTG GAGTCCCGGC CTCTCTACAG CCCCCTGCAG 
CCAGACATTG AACAGGGGAA GCTGCAGATG TGGATCGACC TATTTCCCAA GGCCCTGGGG 
CGTCCCGGAC CTCCCTTCAA CATCACCCCG CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT 
ATCATCTGGA ACACAAAGGA CGTGATCCTG GACGACCTCA GCATCACAGG GGAGAAGATG 
AGTGATATTT ATGTGAAGGG TTGGATGGTT GGCTTTGAAG AGCACAAGCA AAAGACAGAT 
GTGCATTACC GGTCCCTGGG AGGTGAAGGC AACTTTAACT GGAGGTTCAT TTTCCCCTTT 
GACTACCTGC CAGCCGAGCA AGTCTGCACC GTCTCTAAGA AGGATGCTTT CTGGAGGCTG 
GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTATTTC AGATCTGGGA CAATGACAAG 
TTCTCCTTTG ACGACTTTCT GGGCTCCTTG CAGTTGGATC TCAACCACAT GCCCAAGCCT 
GCCAAGACAG CTGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCGGAGTGG 
TTTGTGTCCC TTTTTGAACA GAAAACAGTT AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG 
GGCGAGAAGA AGATCCTGGC AGGCAAGCTG GAAATGACCT TGGAGATCGT AGCAGAGAGT 
GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAAC CCAACATGAA CCCCAAGCTC 
GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCTCCATA CAAGACTATG 
AAGTTCATCC TGTGGCGGCG GTTCCGATGT GCCATTATCC TCTTCATCAT TCTCTTCATC 
CTGCTACTAT TCTTAGGCAT CTTCATCTAT GCTTTCCCGA ACTACGCTGC CATGAAGCTG 
GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432274.1
CDS	51..6368
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432226.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432226.3

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC 
AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC 
CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC 
CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG 
AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC 
TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG 
AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG 
CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGCTTGCAGA TGGTCTGTCG 
GGCTCTGCCC CCACTAACAT GGCATCTTCT CCATCCAGTC CTCATGAGGA AGAGTTCATC 
GACTGGTGGA GCAAATTCTT TGCCTCCACA GGGGAGAGGG AAAAGTGCGG CTCATACTTG 
GAGAAGGGCT TTGACACTCT AAAGGTCTAT GACACACAAC TGGAGAACGT GGAGGCCTTT 
GAGGGCCTGT CTGACTTTTG TAACACCTTC AAGCTCTACC GGGGCAAGAC TCAGGAGGAG 
ACAGAGGACC CATCTGTTAT TGGGGAGTTT AAGGGCCTCT TCAAAATCTA TCCCCTCCCA 
GAAGACCCGG CTGTTCCCAT GCCCCCGAGA CAATTCCACC AGCTGGCTGC CCAAGGGCCT 
CAGGAGTGCC TGGTCCGCAT CTATATCATC CGAGCATTTG GCCTTCAGCC CAAGGACCCC 
AATGGGAAGT GTGATCCTTA CATCAAGATC TCCATAGGGA AGAAATCAGT GAGTGACCAG 
GATAACTACA TTCCCTGTAC TCTGGAGCCT GTGTTTGGAA AGATGTTCGA GCTGACCTGC 
ACTCTGCCTC TGGAGAAGGA CCTGAAGATC ACACTCTATG ACTATGACCT CCTCTCCAAA 
GATGAAAAGA TTGGGGAGAC AGTCATTGAC CTGGAGGATA GGCTGCTGTC CAAGTTTGGG 
GCTCGCTGCG GACTCCCACA GACGTACTGT GTCTCTGGAC CCAACCAGTG GAGGGACCAG 
CTCCGCCCCT CCCAGCTTCT CCACCTCTTC TGCCAGCAGC GCAGACTCAA GGCTCCTGTG 
TACCTCACGG ACCACGTGGT GTTCCAGGAT AAAGAATACA GCATTGAAGA AATAGAGGCT 
GGCCGAGTCC CGAACCCACA CCTGGGCCCA GTGGAAGAGC GTCTGGCCTT GCATGTCCTC 
CAGCAGCAGG GCCTGGTCCC TGAGCATGTG GAGTCCCGGC CTCTCTACAG CCCCCTGCAG 
CCAGACATTG AACAGGGGAA GCTGCAGATG TGGATCGACC TATTTCCCAA GGCCCTGGGG 
CGTCCCGGAC CTCCCTTCAA CATCACCCCG CGGAGAGCCA GAAGGTTTTT CCTGCGTTGT 
ATCATCTGGA ACACAAAGGA CGTGATCCTG GACGACCTCA GCATCACAGG GGAGAAGATG 
AGTGATATTT ATGTGAAGGG TTGGATGGTT GGCTTTGAAG AGCACAAGCA AAAGACAGAT 
GTGCATTACC GGTCCCTGGG AGGTGAAGGC AACTTTAACT GGAGGTTCAT TTTCCCCTTT 
GACTACCTGC CAGCCGAGCA AGTCTGCACC GTCTCTAAGA AGGATGCTTT CTGGAGGCTG 
GACAAGACTG AGAGCAAAAT CCCAGCACGA GTGGTATTTC AGATCTGGGA CAATGACAAG 
TTCTCCTTTG ACGACTTTCT GGGCTCCTTG CAGTTGGATC TCAACCACAT GCCCAAGCCT 
GCCAAGACAG CTGAGAAGTG CTCCTTGGAC CAGCTGGATG ACACTTTCCA CCCGGAGTGG 
TTTGTGTCCC TTTTTGAACA GAAAACAGTT AAGGGCTGGT GGCCCTGCGT AGCAGAAGAG 
GGCGAGAAGA AGATCCTGGC AGGCAAGCTG GAAATGACCT TGGAGATCGT AGCAGAGAGT 
GAGCATGAGG AGCGGCCTGC TGGCCAGGGC CGGGATGAAC CCAACATGAA CCCCAAGCTC 
GAGGACCCAA GGCGCCCTGA CACCTCCTTC CTCTGGTTCA CCTCTCCATA CAAGACTATG 
AAGTTCATCC TGTGGCGGCG GTTCCGATGT GCCATTATCC TCTTCATCAT TCTCTTCATC 
CTGCTACTAT TCTTAGGCAT CTTCATCTAT GCTTTCCCGA ACTACGCTGC CATGAAGCTG 
GTGAAGCCCT TCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432222.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432270.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA.

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CloneID	OCe19211
Clone ID Related Accession (Same CDS sequence)	XM_003432222.3 , XM_003432222.4
Accession Version	XM_003432222.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC 
ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG 
GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT 
GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC 
TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGC TTGCAGATGG TCTGTCGGGC 
TCTGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCCTC ATGAGGAAGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA 
GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA 
GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG 
GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT 
GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC 
CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC 
CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA 
GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT 
CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT 
GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG 
CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC 
GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG 
TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG 
CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432270.1
CDS	439..6753
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432222.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432222.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC 
ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG 
GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT 
GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC 
TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGC TTGCAGATGG TCTGTCGGGC 
TCTGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCCTC ATGAGGAAGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA 
GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA 
GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG 
GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT 
GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC 
CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC 
CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA 
GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT 
CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT 
GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG 
CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC 
GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG 
TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG 
CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432222.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432270.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X15, mRNA.

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CloneID	OCe19211
Clone ID Related Accession (Same CDS sequence)	XM_003432222.3 , XM_003432222.4
Accession Version	XM_003432222.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432222.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC 
ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG 
GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT 
GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC 
TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGC TTGCAGATGG TCTGTCGGGC 
TCTGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCCTC ATGAGGAAGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA 
GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA 
GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG 
GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT 
GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC 
CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC 
CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA 
GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT 
CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT 
GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG 
CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC 
GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG 
TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG 
CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432270.1
CDS	405..6719
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432222.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432222.4

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC 
ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG 
GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT 
GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC 
TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGC TTGCAGATGG TCTGTCGGGC 
TCTGCCCCCA CTAACATGGC ATCTTCTCCA TCCAGTCCTC ATGAGGAAGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA 
GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA 
GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG 
GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT 
GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC 
CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC 
CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA 
GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT 
CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT 
GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG 
CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC 
GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG 
TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG 
CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432233.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432281.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA.

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CloneID	OCe19212
Clone ID Related Accession (Same CDS sequence)	XM_003432233.3 , XM_003432233.4
Accession Version	XM_003432233.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432233.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC 
ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG 
GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA 
GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC 
TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC 
TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC 
CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC 
ATCGATGACA AGGAGCCCCT CATCCCCATG CAGGAGGAAG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA 
TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT 
GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA 
CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC 
CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT 
CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT 
GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG 
TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC 
TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432281.1
CDS	51..6356
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432233.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432233.3

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC 
ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG 
GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA 
GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC 
TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC 
TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC 
CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC 
ATCGATGACA AGGAGCCCCT CATCCCCATG CAGGAGGAAG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA 
TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT 
GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA 
CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC 
CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT 
CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT 
GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG 
TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC 
TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432233.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432281.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X16, mRNA.

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CloneID	OCe19212
Clone ID Related Accession (Same CDS sequence)	XM_003432233.3 , XM_003432233.4
Accession Version	XM_003432233.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6306bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432233.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC 
ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG 
GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA 
GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC 
TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC 
TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC 
CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC 
ATCGATGACA AGGAGCCCCT CATCCCCATG CAGGAGGAAG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA 
TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT 
GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA 
CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC 
CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT 
CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT 
GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG 
TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC 
TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432281.1
CDS	23..6328
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432233.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432233.4

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG GTGGGGGACA GAGCCGGGCT 
GAGACTTGGT CCCTGCTCAG TGACAGCACC GTGGATACAA GATACTCTGG AAAGAAGTGG 
CTGGCCCCCA CGGACACAGG AGGAGAGGAG GACACTGAGG ACCAGGGGCT CACTGGAGAC 
GAGGGAGAGC CATTACTGGA TCAGAGTGGC GTCCCAGGCC CTGGGGCTCC CACCACCCCG 
AGGAAGCCAC CTTCCCATCT GCCCCCCTAC CACCCTGGGA GCAAGAGGAA AAGAAGTGCT 
CCCGCAGCCA GAAAGCTGCT GTCCGACAAA CCGCAGGACT TCCAGATCAG AGTGCAAGTG 
ATCGAGGGCC GCCAGCTGCC AGGGGTGAAT ATCAAGCCTG TGATCAAGGT CACTGCTGCT 
GGGCAGACCA AGAGGACACG GATCCACAAG GGAAACAGCC CACTATTTAA CGAGACTCTT 
TTCTTCAATG TGTTTGACTC TCCTTCGGAG CTGTTTGATG AGCCCATCTT TATCACGGTC 
GTAGACTCCC GTTCTCTCAG GACAGATGCT CTCATTGGGG AATTCCGGAT GGATGTGGGT 
ACCATCTACA GAGAGCCCCG ACATGCTTAT CTGAGGAAGT GGCTGCTACT CTCCAACCCT 
GATGACTTCT CTGTTGGGGC CAGAGGTTAC CTGAAAGCAA GCCTTTATGT GCTGGGGCCC 
GGAGATGAAG CGCCTCTGGA GAGAAAGGAC CCCTCTGAAG ACAAGGAGGA CATTGAAAAC 
AATCTGCTCA GGCCAACTGG CGTGGCCCTG CGAGGAGCCC ACTTCTGCCT GAAGATCTTC 
AGAGCGGAGG ACTTACCACA AATGGATGAT GCCGTGATGG ACAATGTGAA GCAGATCTTT 
GGCTTTGACA GCAACAAGAA GAATTTGGTA GATCCCTTCA TGGAAGTCAG CTTTGCGGGG 
AAAATGCTCT GCAGCAAGAT CCTGGAGAAG ACAGCCAACC CTCAGTGGAA CCAGAGCATC 
ACTCTGCCTG CCATGTTTCC CTCCATGTGT GAAAAAATGA AGATTCGCGT CATGGACTGG 
GACCGCCTTA CCCACAATGA CATCGTGGCC ACCACCTACC TGAGTATGTC GAAAATCTCT 
GCCCCTGGAG GAGAAATAGA AGTGGATGAC CATCTGGGCT TCCTTCCCAC CTTCGGGCCC 
TGCTTCGTCA ATCTCTATGG CAGCCCCAGG GAATTCACTG GCTTCCCGGA CCCCTACGCG 
GAACTCAACA TGGGCAAGGG GGAAGGCGTG GCCTACCGAG GTCGGCTTCT GCTCTCCCTG 
GAGACCAAGC TGGTGGAGCA CAGCGGCGAG CAAAGGGTGG AGGACCTCCC TGCTGATGAC 
ATCCTTCGGG TGGAGAAATA CCTCCGGAGG CGCAAGTACT CTCTCTTTGC CGCCTTCTAC 
TCGGCCACCA TGCTCCAGGA TGTCGACGAT GCCATCCAGT TTGAGGTCAG CATTGGGAAC 
TATGGCAACA AGTTCGACAC GACCTGTCTG CCACTGGCCT CCACCACCCA GTACAGCCGG 
GCAGTCTTTG ATGGGTGCCA CTACTACTAC TTGCCCTGGG GCAATGTGAA GCCTGTGGTG 
GTGCTGTCAT CCTACTGGGA AGACATCAGC CACAGAGTCG AGGCTCAGAA CCAGCTGCTC 
AGGATCGCTG ACCATCTGGA AGCTGGCCTG GAGCAAGTCC ACTTGGCTTT AAAGGCGCAG 
TTCAACACAG AGGATGTGGA CTCCCTGTTG GCCCAGCTGA TGGATGAGCT CATCTCAGAC 
TGCAGCCAGC CCCTGGGCAA TGTCCAAGAG ATACCCTCTG CCACCCACCT GGACCGGCAC 
CTGTACAGGC TGCGCACCCG TCACCTAGGC CAGATTACTG AGGCGGCCCT GGCTCTGAAA 
CTCGGGCATG GCAAGCTCCC TGCCGCCCTG GAGCAGGCTG AAGACTGGCT CCTGCGTCTC 
CGTGCCCTGG CTGATGAGCC CCAGAACAGC CTGCCCGACA TCATCATCTG GATGCTGCAG 
GGAAACAAGC GAGTGGCGTA CCAGCGGGTG CCTGCGCACG AGGTCCTCTT CTCCCGGCGG 
GGCGCCAGCT ACTGTGGCAA GAACTGTGGG AAGCTGCAGA CGATATTTCT GAAATATCCC 
ATGGAGGGGG TGCCCGGAGC CCGGATGCCA GTACAAATAC GGGTGAAACT CTGGTTTGGG 
CTTTCTGTGG ATGAGAAGGA GTTCAACCAG TTTGCTGAGG GAAAGCTGTC CGTCTTTGCT 
GAAACTTATG AGAACCAGAC CAAACTGGCC CTGGTTGGGA ACTGGGGCAC AACAGGCCTC 
ACCTACCCCA AGTTTTCTGA TGTCATGGGC AAGATCAAGC TACCCAAGGA TAGCTTCCGC 
CCCTCAGCTG GCTGGACCTG GGCCGGAGAC TGGTTCGTGT GTCCAGAGAA GACCCTACTC 
CATGACATGG ACGCGGGCCA CCTGAGTTTT GTGGAGGAGG TGTTTGAGAA CCAGACCCGG 
CTCCCTGGAG GCCAGTGGAT CTACATGAGT GACAACTACA CAGATGTGAA TGGGGATAAG 
GTGCTTCCTA AGGATGACAT TGAGTGCCCA CTGGGCTGGA AGTGGGAAGA TGAAGAGTGG 
TCCACGGACC TCAATCGGGC CGTCGATGAG CAAGGCTGGG AGTACAGTAT CACCATCCCC 
CCGGATCGGA AGCCAAAGCA CTGGGTTCCT GCTGAGAAGA TGTATTACAC GCACCGTCGG 
CGGCGCTGGG TCCGCCTGCG CAGAAGGGAC CTCAGCCAGA TGGAGGCATT GAAGAGGCAC 
AGACAGGCAG AGGCAGAGGG TGAGGGCTGG GAGTACGCCT CGCTCTTGGG CTGGAAGTTC 
CACCTGGAGT ACCGCAAGAC TGATGCTTTC CGCCGCCGCC GCTGGCGCCG CCGCATGGAA 
CCGCTGGAGA AGACGGGGCC TGCAGCCGTG TTTGCCCTTG AGGGGGCTCT GGGTGGTGTG 
GTAGATGACA GGAGTGAAGA TTCCGTGTCC GTGTCCACCT TGAGCTTTGG AGTGAACAGA 
CCCACGATTT CCTGCATCTT TGATTATGGG AACCGCTACC ACCTACGCTG CTACATGTAC 
CAGGCTCGGG ACCTGCCCTC GATGGACAAG GACTCTTTTT CTGATCCCTA CGCCATCGTC 
TCCTTCCTGC ACCAGAGCCA GAAGACAGTG GTGGCGAAGA ACACTCTGAA CCCCACCTGG 
GACCAGACGC TTATCTTCTA TGAGATTGAG ATCTTTGGTG AGCCAGCCAG TATCGCTGAG 
CAGCCGCCCA GCATTGTGGT GGAGCTGTAT GACCACGACA CCTACGGTGC AGATGAGTTT 
ATGGGACGCT GCATTTGTCA GCCCAGCCTG GAGCGGACAC CCCGGCTGGC CTGGTTCCCA 
CTGATTAGGG GCAGCCAGTC GGCGGGGGAA CTGCTGGCCT CTTTTGAGCT CATCCAGAGA 
GAAAAGCCGG CCATTCACCA TATTCCTGGT TTTGAGGTAC AGGATATGTC AGGAATCCTG 
GAAGAGTCAG AGGACATAGA CCTGCCCTAC CCGCCACCTC AGAGGGAGGC CAACATCTTC 
ATGGTCCCCC AGAACATCAA GCCTGTTCTC CAGCGTACTG CCATAGAGAT CCTGGCGTGG 
GGCCTGCGGA ACATGAAGAG CTACCAGCTA GCCAGCATCT CCTCCCCTAG CCTTGTGGTA 
GAGTGCGGGG GCCAGACAGT GCAGTCCTGT GTCATCAAGA ACCTCCGGAA GAACCCCAAC 
TTCGACATCT GTACCCTTTT CATGGAAGTG ATGCTGCCCA AGGAGGAGGT CTACTGTCCC 
TCCATCGTGA TCAAGGTCAT TGATAACCGC CAGTTTGGCC GCCGGCCTGT GGTGGGCCAG 
TGTACCATCC GTTCCCTGGA GAGCTTCATG TGTGACCTGT ACTCCGAGGA GAATCCGTCC 
CCACAGGGCG GCCCAGATGA TGTGAGTTTA CTCAGTCCTG GGGAAGACGT GCTCATTGAC 
ATCGATGACA AGGAGCCCCT CATCCCCATG CAGGAGGAAG AGTTCATCGA CTGGTGGAGC 
AAATTCTTTG CCTCCACAGG GGAGAGGGAA AAGTGCGGCT CATACTTGGA GAAGGGCTTT 
GACACTCTAA AGGTCTATGA CACACAACTG GAGAACGTGG AGGCCTTTGA GGGCCTGTCT 
GACTTTTGTA ACACCTTCAA GCTCTACCGG GGCAAGACTC AGGAGGAGAC AGAGGACCCA 
TCTGTTATTG GGGAGTTTAA GGGCCTCTTC AAAATCTATC CCCTCCCAGA AGACCCGGCT 
GTTCCCATGC CCCCGAGACA ATTCCACCAG CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG 
GTCCGCATCT ATATCATCCG AGCATTTGGC CTTCAGCCCA AGGACCCCAA TGGGAAGTGT 
GATCCTTACA TCAAGATCTC CATAGGGAAG AAATCAGTGA GTGACCAGGA TAACTACATT 
CCCTGTACTC TGGAGCCTGT GTTTGGAAAG ATGTTCGAGC TGACCTGCAC TCTGCCTCTG 
GAGAAGGACC TGAAGATCAC ACTCTATGAC TATGACCTCC TCTCCAAAGA TGAAAAGATT 
GGGGAGACAG TCATTGACCT GGAGGATAGG CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA 
CTCCCACAGA CGTACTGTGT CTCTGGACCC AACCAGTGGA GGGACCAGCT CCGCCCCTCC 
CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC AGACTCAAGG CTCCTGTGTA CCTCACGGAC 
CACGTGGTGT TCCAGGATAA AGAATACAGC ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG 
AACCCACACC TGGGCCCAGT GGAAGAGCGT CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC 
CTGGTCCCTG AGCATGTGGA GTCCCGGCCT CTCTACAGCC CCCTGCAGCC AGACATTGAA 
CAGGGGAAGC TGCAGATGTG GATCGACCTA TTTCCCAAGG CCCTGGGGCG TCCCGGACCT 
CCCTTCAACA TCACCCCGCG GAGAGCCAGA AGGTTTTTCC TGCGTTGTAT CATCTGGAAC 
ACAAAGGACG TGATCCTGGA CGACCTCAGC ATCACAGGGG AGAAGATGAG TGATATTTAT 
GTGAAGGGTT GGATGGTTGG CTTTGAAGAG CACAAGCAAA AGACAGATGT GCATTACCGG 
TCCCTGGGAG GTGAAGGCAA CTTTAACTGG AGGTTCATTT TCCCCTTTGA CTACCTGCCA 
GCCGAGCAAG TCTGCACCGT CTCTAAGAAG GATGCTTTCT GGAGGCTGGA CAAGACTGAG 
AGCAAAATCC CAGCACGAGT GGTATTTCAG ATCTGGGACA ATGACAAGTT CTCCTTTGAC 
GACTTTCTGG GCTCCTTGCA GTTGGATCTC AACCACATGC CCAAGCCTGC CAAGACAGCT 
GAGAAGTGCT CCTTGGACCA GCTGGATGAC ACTTTCCACC CGGAGTGGTT TGTGTCCCTT 
TTTGAACAGA AAACAGTTAA GGGCTGGTGG CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG 
ATCCTGGCAG GCAAGCTGGA AATGACCTTG GAGATCGTAG CAGAGAGTGA GCATGAGGAG 
CGGCCTGCTG GCCAGGGCCG GGATGAACCC AACATGAACC CCAAGCTCGA GGACCCAAGG 
CGCCCTGACA CCTCCTTCCT CTGGTTCACC TCTCCATACA AGACTATGAA GTTCATCCTG 
TGGCGGCGGT TCCGATGTGC CATTATCCTC TTCATCATTC TCTTCATCCT GCTACTATTC 
TTAGGCATCT TCATCTATGC TTTCCCGAAC TACGCTGCCA TGAAGCTGGT GAAGCCCTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432228.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432276.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA.

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CloneID	OCe19213
Clone ID Related Accession (Same CDS sequence)	XM_003432228.3 , XM_003432228.4
Accession Version	XM_003432228.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432228.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG 
GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC 
CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG 
TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATGCAG GAGGAAGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT 
GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT 
CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC 
CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC 
CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC 
GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC 
TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG 
AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG 
CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA 
GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432276.1
CDS	439..6741
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432228.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432228.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG 
GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC 
CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG 
TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATGCAG GAGGAAGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT 
GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT 
CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC 
CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC 
CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC 
GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC 
TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG 
AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG 
CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA 
GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432228.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432276.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X17, mRNA.

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CloneID	OCe19213
Clone ID Related Accession (Same CDS sequence)	XM_003432228.3 , XM_003432228.4
Accession Version	XM_003432228.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6303bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432228.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG 
GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC 
CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG 
TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATGCAG GAGGAAGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT 
GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT 
CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC 
CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC 
CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC 
GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC 
TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG 
AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG 
CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA 
GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432276.1
CDS	405..6707
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGAGGGQSRAETWSLLSDSTVDTRYSGKKWLAPTDTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432228.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432228.4

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGGTG GGGGACAGAG CCGGGCTGAG 
ACTTGGTCCC TGCTCAGTGA CAGCACCGTG GATACAAGAT ACTCTGGAAA GAAGTGGCTG 
GCCCCCACGG ACACAGGAGG AGAGGAGGAC ACTGAGGACC AGGGGCTCAC TGGAGACGAG 
GGAGAGCCAT TACTGGATCA GAGTGGCGTC CCAGGCCCTG GGGCTCCCAC CACCCCGAGG 
AAGCCACCTT CCCATCTGCC CCCCTACCAC CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC 
GCAGCCAGAA AGCTGCTGTC CGACAAACCG CAGGACTTCC AGATCAGAGT GCAAGTGATC 
GAGGGCCGCC AGCTGCCAGG GGTGAATATC AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG 
CAGACCAAGA GGACACGGAT CCACAAGGGA AACAGCCCAC TATTTAACGA GACTCTTTTC 
TTCAATGTGT TTGACTCTCC TTCGGAGCTG TTTGATGAGC CCATCTTTAT CACGGTCGTA 
GACTCCCGTT CTCTCAGGAC AGATGCTCTC ATTGGGGAAT TCCGGATGGA TGTGGGTACC 
ATCTACAGAG AGCCCCGACA TGCTTATCTG AGGAAGTGGC TGCTACTCTC CAACCCTGAT 
GACTTCTCTG TTGGGGCCAG AGGTTACCTG AAAGCAAGCC TTTATGTGCT GGGGCCCGGA 
GATGAAGCGC CTCTGGAGAG AAAGGACCCC TCTGAAGACA AGGAGGACAT TGAAAACAAT 
CTGCTCAGGC CAACTGGCGT GGCCCTGCGA GGAGCCCACT TCTGCCTGAA GATCTTCAGA 
GCGGAGGACT TACCACAAAT GGATGATGCC GTGATGGACA ATGTGAAGCA GATCTTTGGC 
TTTGACAGCA ACAAGAAGAA TTTGGTAGAT CCCTTCATGG AAGTCAGCTT TGCGGGGAAA 
ATGCTCTGCA GCAAGATCCT GGAGAAGACA GCCAACCCTC AGTGGAACCA GAGCATCACT 
CTGCCTGCCA TGTTTCCCTC CATGTGTGAA AAAATGAAGA TTCGCGTCAT GGACTGGGAC 
CGCCTTACCC ACAATGACAT CGTGGCCACC ACCTACCTGA GTATGTCGAA AATCTCTGCC 
CCTGGAGGAG AAATAGAAGT GGATGACCAT CTGGGCTTCC TTCCCACCTT CGGGCCCTGC 
TTCGTCAATC TCTATGGCAG CCCCAGGGAA TTCACTGGCT TCCCGGACCC CTACGCGGAA 
CTCAACATGG GCAAGGGGGA AGGCGTGGCC TACCGAGGTC GGCTTCTGCT CTCCCTGGAG 
ACCAAGCTGG TGGAGCACAG CGGCGAGCAA AGGGTGGAGG ACCTCCCTGC TGATGACATC 
CTTCGGGTGG AGAAATACCT CCGGAGGCGC AAGTACTCTC TCTTTGCCGC CTTCTACTCG 
GCCACCATGC TCCAGGATGT CGACGATGCC ATCCAGTTTG AGGTCAGCAT TGGGAACTAT 
GGCAACAAGT TCGACACGAC CTGTCTGCCA CTGGCCTCCA CCACCCAGTA CAGCCGGGCA 
GTCTTTGATG GGTGCCACTA CTACTACTTG CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG 
CTGTCATCCT ACTGGGAAGA CATCAGCCAC AGAGTCGAGG CTCAGAACCA GCTGCTCAGG 
ATCGCTGACC ATCTGGAAGC TGGCCTGGAG CAAGTCCACT TGGCTTTAAA GGCGCAGTTC 
AACACAGAGG ATGTGGACTC CCTGTTGGCC CAGCTGATGG ATGAGCTCAT CTCAGACTGC 
AGCCAGCCCC TGGGCAATGT CCAAGAGATA CCCTCTGCCA CCCACCTGGA CCGGCACCTG 
TACAGGCTGC GCACCCGTCA CCTAGGCCAG ATTACTGAGG CGGCCCTGGC TCTGAAACTC 
GGGCATGGCA AGCTCCCTGC CGCCCTGGAG CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT 
GCCCTGGCTG ATGAGCCCCA GAACAGCCTG CCCGACATCA TCATCTGGAT GCTGCAGGGA 
AACAAGCGAG TGGCGTACCA GCGGGTGCCT GCGCACGAGG TCCTCTTCTC CCGGCGGGGC 
GCCAGCTACT GTGGCAAGAA CTGTGGGAAG CTGCAGACGA TATTTCTGAA ATATCCCATG 
GAGGGGGTGC CCGGAGCCCG GATGCCAGTA CAAATACGGG TGAAACTCTG GTTTGGGCTT 
TCTGTGGATG AGAAGGAGTT CAACCAGTTT GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA 
ACTTATGAGA ACCAGACCAA ACTGGCCCTG GTTGGGAACT GGGGCACAAC AGGCCTCACC 
TACCCCAAGT TTTCTGATGT CATGGGCAAG ATCAAGCTAC CCAAGGATAG CTTCCGCCCC 
TCAGCTGGCT GGACCTGGGC CGGAGACTGG TTCGTGTGTC CAGAGAAGAC CCTACTCCAT 
GACATGGACG CGGGCCACCT GAGTTTTGTG GAGGAGGTGT TTGAGAACCA GACCCGGCTC 
CCTGGAGGCC AGTGGATCTA CATGAGTGAC AACTACACAG ATGTGAATGG GGATAAGGTG 
CTTCCTAAGG ATGACATTGA GTGCCCACTG GGCTGGAAGT GGGAAGATGA AGAGTGGTCC 
ACGGACCTCA ATCGGGCCGT CGATGAGCAA GGCTGGGAGT ACAGTATCAC CATCCCCCCG 
GATCGGAAGC CAAAGCACTG GGTTCCTGCT GAGAAGATGT ATTACACGCA CCGTCGGCGG 
CGCTGGGTCC GCCTGCGCAG AAGGGACCTC AGCCAGATGG AGGCATTGAA GAGGCACAGA 
CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG TACGCCTCGC TCTTGGGCTG GAAGTTCCAC 
CTGGAGTACC GCAAGACTGA TGCTTTCCGC CGCCGCCGCT GGCGCCGCCG CATGGAACCG 
CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA 
GATGACAGGA GTGAAGATTC CGTGTCCGTG TCCACCTTGA GCTTTGGAGT GAACAGACCC 
ACGATTTCCT GCATCTTTGA TTATGGGAAC CGCTACCACC TACGCTGCTA CATGTACCAG 
GCTCGGGACC TGCCCTCGAT GGACAAGGAC TCTTTTTCTG ATCCCTACGC CATCGTCTCC 
TTCCTGCACC AGAGCCAGAA GACAGTGGTG GCGAAGAACA CTCTGAACCC CACCTGGGAC 
CAGACGCTTA TCTTCTATGA GATTGAGATC TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG 
CCGCCCAGCA TTGTGGTGGA GCTGTATGAC CACGACACCT ACGGTGCAGA TGAGTTTATG 
GGACGCTGCA TTTGTCAGCC CAGCCTGGAG CGGACACCCC GGCTGGCCTG GTTCCCACTG 
ATTAGGGGCA GCCAGTCGGC GGGGGAACTG CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA 
AAGCCGGCCA TTCACCATAT TCCTGGTTTT GAGGTACAGG ATATGTCAGG AATCCTGGAA 
GAGTCAGAGG ACATAGACCT GCCCTACCCG CCACCTCAGA GGGAGGCCAA CATCTTCATG 
GTCCCCCAGA ACATCAAGCC TGTTCTCCAG CGTACTGCCA TAGAGATCCT GGCGTGGGGC 
CTGCGGAACA TGAAGAGCTA CCAGCTAGCC AGCATCTCCT CCCCTAGCCT TGTGGTAGAG 
TGCGGGGGCC AGACAGTGCA GTCCTGTGTC ATCAAGAACC TCCGGAAGAA CCCCAACTTC 
GACATCTGTA CCCTTTTCAT GGAAGTGATG CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC 
ATCGTGATCA AGGTCATTGA TAACCGCCAG TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT 
ACCATCCGTT CCCTGGAGAG CTTCATGTGT GACCTGTACT CCGAGGAGAA TCCGTCCCCA 
CAGGGCGGCC CAGATGATGT GAGTTTACTC AGTCCTGGGG AAGACGTGCT CATTGACATC 
GATGACAAGG AGCCCCTCAT CCCCATGCAG GAGGAAGAGT TCATCGACTG GTGGAGCAAA 
TTCTTTGCCT CCACAGGGGA GAGGGAAAAG TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC 
ACTCTAAAGG TCTATGACAC ACAACTGGAG AACGTGGAGG CCTTTGAGGG CCTGTCTGAC 
TTTTGTAACA CCTTCAAGCT CTACCGGGGC AAGACTCAGG AGGAGACAGA GGACCCATCT 
GTTATTGGGG AGTTTAAGGG CCTCTTCAAA ATCTATCCCC TCCCAGAAGA CCCGGCTGTT 
CCCATGCCCC CGAGACAATT CCACCAGCTG GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC 
CGCATCTATA TCATCCGAGC ATTTGGCCTT CAGCCCAAGG ACCCCAATGG GAAGTGTGAT 
CCTTACATCA AGATCTCCAT AGGGAAGAAA TCAGTGAGTG ACCAGGATAA CTACATTCCC 
TGTACTCTGG AGCCTGTGTT TGGAAAGATG TTCGAGCTGA CCTGCACTCT GCCTCTGGAG 
AAGGACCTGA AGATCACACT CTATGACTAT GACCTCCTCT CCAAAGATGA AAAGATTGGG 
GAGACAGTCA TTGACCTGGA GGATAGGCTG CTGTCCAAGT TTGGGGCTCG CTGCGGACTC 
CCACAGACGT ACTGTGTCTC TGGACCCAAC CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG 
CTTCTCCACC TCTTCTGCCA GCAGCGCAGA CTCAAGGCTC CTGTGTACCT CACGGACCAC 
GTGGTGTTCC AGGATAAAGA ATACAGCATT GAAGAAATAG AGGCTGGCCG AGTCCCGAAC 
CCACACCTGG GCCCAGTGGA AGAGCGTCTG GCCTTGCATG TCCTCCAGCA GCAGGGCCTG 
GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC TACAGCCCCC TGCAGCCAGA CATTGAACAG 
GGGAAGCTGC AGATGTGGAT CGACCTATTT CCCAAGGCCC TGGGGCGTCC CGGACCTCCC 
TTCAACATCA CCCCGCGGAG AGCCAGAAGG TTTTTCCTGC GTTGTATCAT CTGGAACACA 
AAGGACGTGA TCCTGGACGA CCTCAGCATC ACAGGGGAGA AGATGAGTGA TATTTATGTG 
AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC AAGCAAAAGA CAGATGTGCA TTACCGGTCC 
CTGGGAGGTG AAGGCAACTT TAACTGGAGG TTCATTTTCC CCTTTGACTA CCTGCCAGCC 
GAGCAAGTCT GCACCGTCTC TAAGAAGGAT GCTTTCTGGA GGCTGGACAA GACTGAGAGC 
AAAATCCCAG CACGAGTGGT ATTTCAGATC TGGGACAATG ACAAGTTCTC CTTTGACGAC 
TTTCTGGGCT CCTTGCAGTT GGATCTCAAC CACATGCCCA AGCCTGCCAA GACAGCTGAG 
AAGTGCTCCT TGGACCAGCT GGATGACACT TTCCACCCGG AGTGGTTTGT GTCCCTTTTT 
GAACAGAAAA CAGTTAAGGG CTGGTGGCCC TGCGTAGCAG AAGAGGGCGA GAAGAAGATC 
CTGGCAGGCA AGCTGGAAAT GACCTTGGAG ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG 
CCTGCTGGCC AGGGCCGGGA TGAACCCAAC ATGAACCCCA AGCTCGAGGA CCCAAGGCGC 
CCTGACACCT CCTTCCTCTG GTTCACCTCT CCATACAAGA CTATGAAGTT CATCCTGTGG 
CGGCGGTTCC GATGTGCCAT TATCCTCTTC ATCATTCTCT TCATCCTGCT ACTATTCTTA 
GGCATCTTCA TCTATGCTTT CCCGAACTAC GCTGCCATGA AGCTGGTGAA GCCCTTCAGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432231.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432279.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X18, mRNA.

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CloneID	OCe19214
Clone ID Related Accession (Same CDS sequence)	XM_003432231.4 , XM_003432231.3
Accession Version	XM_003432231.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432231.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA 
TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA 
AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC 
AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC 
ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA 
CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG 
CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC 
AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG 
CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC 
CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA 
CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG 
ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG 
CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG 
CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT 
GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG 
CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA 
CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT 
GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG 
GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG 
ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG 
TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG 
GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC 
AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG 
GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA 
GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT 
GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC 
AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG 
TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC 
CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT 
GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG 
TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC 
CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC 
TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG 
GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC 
TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC 
CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG 
CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG 
CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT 
GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG 
CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG 
CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC 
AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC 
ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG 
CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT 
GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC 
AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG 
CTTGCAGATG GTCTGTCGGG CTCTGCCCCC ACTAACATGG CATCTTCTCC ATCCAGTCCT 
CATGAGGAAG AGTTCATCGA CTGGTGGAGC AAATTCTTTG CCTCCACAGG GGAGAGGGAA 
AAGTGCGGCT CATACTTGGA GAAGGGCTTT GACACTCTAA AGGTCTATGA CACACAACTG 
GAGAACGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTCTACCGG 
GGCAAGACTC AGGAGGAGAC AGAGGACCCA TCTGTTATTG GGGAGTTTAA GGGCCTCTTC 
AAAATCTATC CCCTCCCAGA AGACCCGGCT GTTCCCATGC CCCCGAGACA ATTCCACCAG 
CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG GTCCGCATCT ATATCATCCG AGCATTTGGC 
CTTCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATT CCCTGTACTC TGGAGCCTGT GTTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGATCAC ACTCTATGAC 
TATGACCTCC TCTCCAAAGA TGAAAAGATT GGGGAGACAG TCATTGACCT GGAGGATAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA CTCCCACAGA CGTACTGTGT CTCTGGACCC 
AACCAGTGGA GGGACCAGCT CCGCCCCTCC CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC 
AGACTCAAGG CTCCTGTGTA CCTCACGGAC CACGTGGTGT TCCAGGATAA AGAATACAGC 
ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG AACCCACACC TGGGCCCAGT GGAAGAGCGT 
CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC CTGGTCCCTG AGCATGTGGA GTCCCGGCCT 
CTCTACAGCC CCCTGCAGCC AGACATTGAA CAGGGGAAGC TGCAGATGTG GATCGACCTA 
TTTCCCAAGG CCCTGGGGCG TCCCGGACCT CCCTTCAACA TCACCCCGCG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT CATCTGGAAC ACAAAGGACG TGATCCTGGA CGACCTCAGC 
ATCACAGGGG AGAAGATGAG TGATATTTAT GTGAAGGGTT GGATGGTTGG CTTTGAAGAG 
CACAAGCAAA AGACAGATGT GCATTACCGG TCCCTGGGAG GTGAAGGCAA CTTTAACTGG 
AGGTTCATTT TCCCCTTTGA CTACCTGCCA GCCGAGCAAG TCTGCACCGT CTCTAAGAAG 
GATGCTTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTATTTCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAC GACTTTCTGG GCTCCTTGCA GTTGGATCTC 
AACCACATGC CCAAGCCTGC CAAGACAGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CGGAGTGGTT TGTGTCCCTT TTTGAACAGA AAACAGTTAA GGGCTGGTGG 
CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG ATCCTGGCAG GCAAGCTGGA AATGACCTTG 
GAGATCGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAACCC 
AACATGAACC CCAAGCTCGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT CTGGTTCACC 
TCTCCATACA AGACTATGAA GTTCATCCTG TGGCGGCGGT TCCGATGTGC CATTATCCTC 
TTCATCATTC TCTTCATCCT GCTACTATTC TTAGGCATCT TCATCTATGC TTTCCCGAAC 
TACGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432279.1
CDS	23..6298
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432231.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432231.4

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA 
TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA 
AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC 
AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC 
ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA 
CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG 
CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC 
AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG 
CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC 
CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA 
CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG 
ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG 
CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG 
CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT 
GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG 
CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA 
CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT 
GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG 
GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG 
ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG 
TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG 
GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC 
AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG 
GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA 
GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT 
GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC 
AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG 
TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC 
CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT 
GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG 
TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC 
CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC 
TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG 
GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC 
TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC 
CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG 
CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG 
CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT 
GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG 
CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG 
CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC 
AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC 
ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG 
CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT 
GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC 
AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG 
CTTGCAGATG GTCTGTCGGG CTCTGCCCCC ACTAACATGG CATCTTCTCC ATCCAGTCCT 
CATGAGGAAG AGTTCATCGA CTGGTGGAGC AAATTCTTTG CCTCCACAGG GGAGAGGGAA 
AAGTGCGGCT CATACTTGGA GAAGGGCTTT GACACTCTAA AGGTCTATGA CACACAACTG 
GAGAACGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTCTACCGG 
GGCAAGACTC AGGAGGAGAC AGAGGACCCA TCTGTTATTG GGGAGTTTAA GGGCCTCTTC 
AAAATCTATC CCCTCCCAGA AGACCCGGCT GTTCCCATGC CCCCGAGACA ATTCCACCAG 
CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG GTCCGCATCT ATATCATCCG AGCATTTGGC 
CTTCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATT CCCTGTACTC TGGAGCCTGT GTTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGATCAC ACTCTATGAC 
TATGACCTCC TCTCCAAAGA TGAAAAGATT GGGGAGACAG TCATTGACCT GGAGGATAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA CTCCCACAGA CGTACTGTGT CTCTGGACCC 
AACCAGTGGA GGGACCAGCT CCGCCCCTCC CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC 
AGACTCAAGG CTCCTGTGTA CCTCACGGAC CACGTGGTGT TCCAGGATAA AGAATACAGC 
ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG AACCCACACC TGGGCCCAGT GGAAGAGCGT 
CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC CTGGTCCCTG AGCATGTGGA GTCCCGGCCT 
CTCTACAGCC CCCTGCAGCC AGACATTGAA CAGGGGAAGC TGCAGATGTG GATCGACCTA 
TTTCCCAAGG CCCTGGGGCG TCCCGGACCT CCCTTCAACA TCACCCCGCG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT CATCTGGAAC ACAAAGGACG TGATCCTGGA CGACCTCAGC 
ATCACAGGGG AGAAGATGAG TGATATTTAT GTGAAGGGTT GGATGGTTGG CTTTGAAGAG 
CACAAGCAAA AGACAGATGT GCATTACCGG TCCCTGGGAG GTGAAGGCAA CTTTAACTGG 
AGGTTCATTT TCCCCTTTGA CTACCTGCCA GCCGAGCAAG TCTGCACCGT CTCTAAGAAG 
GATGCTTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTATTTCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAC GACTTTCTGG GCTCCTTGCA GTTGGATCTC 
AACCACATGC CCAAGCCTGC CAAGACAGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CGGAGTGGTT TGTGTCCCTT TTTGAACAGA AAACAGTTAA GGGCTGGTGG 
CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG ATCCTGGCAG GCAAGCTGGA AATGACCTTG 
GAGATCGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAACCC 
AACATGAACC CCAAGCTCGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT CTGGTTCACC 
TCTCCATACA AGACTATGAA GTTCATCCTG TGGCGGCGGT TCCGATGTGC CATTATCCTC 
TTCATCATTC TCTTCATCCT GCTACTATTC TTAGGCATCT TCATCTATGC TTTCCCGAAC 
TACGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432231.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432279.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA.

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CloneID	OCe19214
Clone ID Related Accession (Same CDS sequence)	XM_003432231.4 , XM_003432231.3
Accession Version	XM_003432231.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432231.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA 
TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA 
AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC 
AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC 
ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA 
CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG 
CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC 
AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG 
CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC 
CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA 
CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG 
ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG 
CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG 
CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT 
GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG 
CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA 
CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT 
GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG 
GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG 
ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG 
TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG 
GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC 
AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG 
GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA 
GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT 
GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC 
AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG 
TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC 
CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT 
GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG 
TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC 
CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC 
TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG 
GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC 
TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC 
CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG 
CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG 
CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT 
GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG 
CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG 
CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC 
AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC 
ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG 
CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT 
GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC 
AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG 
CTTGCAGATG GTCTGTCGGG CTCTGCCCCC ACTAACATGG CATCTTCTCC ATCCAGTCCT 
CATGAGGAAG AGTTCATCGA CTGGTGGAGC AAATTCTTTG CCTCCACAGG GGAGAGGGAA 
AAGTGCGGCT CATACTTGGA GAAGGGCTTT GACACTCTAA AGGTCTATGA CACACAACTG 
GAGAACGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTCTACCGG 
GGCAAGACTC AGGAGGAGAC AGAGGACCCA TCTGTTATTG GGGAGTTTAA GGGCCTCTTC 
AAAATCTATC CCCTCCCAGA AGACCCGGCT GTTCCCATGC CCCCGAGACA ATTCCACCAG 
CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG GTCCGCATCT ATATCATCCG AGCATTTGGC 
CTTCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATT CCCTGTACTC TGGAGCCTGT GTTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGATCAC ACTCTATGAC 
TATGACCTCC TCTCCAAAGA TGAAAAGATT GGGGAGACAG TCATTGACCT GGAGGATAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA CTCCCACAGA CGTACTGTGT CTCTGGACCC 
AACCAGTGGA GGGACCAGCT CCGCCCCTCC CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC 
AGACTCAAGG CTCCTGTGTA CCTCACGGAC CACGTGGTGT TCCAGGATAA AGAATACAGC 
ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG AACCCACACC TGGGCCCAGT GGAAGAGCGT 
CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC CTGGTCCCTG AGCATGTGGA GTCCCGGCCT 
CTCTACAGCC CCCTGCAGCC AGACATTGAA CAGGGGAAGC TGCAGATGTG GATCGACCTA 
TTTCCCAAGG CCCTGGGGCG TCCCGGACCT CCCTTCAACA TCACCCCGCG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT CATCTGGAAC ACAAAGGACG TGATCCTGGA CGACCTCAGC 
ATCACAGGGG AGAAGATGAG TGATATTTAT GTGAAGGGTT GGATGGTTGG CTTTGAAGAG 
CACAAGCAAA AGACAGATGT GCATTACCGG TCCCTGGGAG GTGAAGGCAA CTTTAACTGG 
AGGTTCATTT TCCCCTTTGA CTACCTGCCA GCCGAGCAAG TCTGCACCGT CTCTAAGAAG 
GATGCTTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTATTTCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAC GACTTTCTGG GCTCCTTGCA GTTGGATCTC 
AACCACATGC CCAAGCCTGC CAAGACAGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CGGAGTGGTT TGTGTCCCTT TTTGAACAGA AAACAGTTAA GGGCTGGTGG 
CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG ATCCTGGCAG GCAAGCTGGA AATGACCTTG 
GAGATCGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAACCC 
AACATGAACC CCAAGCTCGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT CTGGTTCACC 
TCTCCATACA AGACTATGAA GTTCATCCTG TGGCGGCGGT TCCGATGTGC CATTATCCTC 
TTCATCATTC TCTTCATCCT GCTACTATTC TTAGGCATCT TCATCTATGC TTTCCCGAAC 
TACGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432279.1
CDS	51..6326
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432231.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432231.3

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA 
TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA 
AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC 
AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC 
ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA 
CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG 
CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC 
AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG 
CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC 
CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA 
CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG 
ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG 
CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG 
CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT 
GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG 
CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA 
CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT 
GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG 
GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG 
ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG 
TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG 
GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC 
AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG 
GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA 
GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT 
GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC 
AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG 
TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC 
CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT 
GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG 
TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC 
CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC 
TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG 
GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC 
TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC 
CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG 
CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG 
CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT 
GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG 
CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG 
CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC 
AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC 
ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG 
CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT 
GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC 
AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG 
CTTGCAGATG GTCTGTCGGG CTCTGCCCCC ACTAACATGG CATCTTCTCC ATCCAGTCCT 
CATGAGGAAG AGTTCATCGA CTGGTGGAGC AAATTCTTTG CCTCCACAGG GGAGAGGGAA 
AAGTGCGGCT CATACTTGGA GAAGGGCTTT GACACTCTAA AGGTCTATGA CACACAACTG 
GAGAACGTGG AGGCCTTTGA GGGCCTGTCT GACTTTTGTA ACACCTTCAA GCTCTACCGG 
GGCAAGACTC AGGAGGAGAC AGAGGACCCA TCTGTTATTG GGGAGTTTAA GGGCCTCTTC 
AAAATCTATC CCCTCCCAGA AGACCCGGCT GTTCCCATGC CCCCGAGACA ATTCCACCAG 
CTGGCTGCCC AAGGGCCTCA GGAGTGCCTG GTCCGCATCT ATATCATCCG AGCATTTGGC 
CTTCAGCCCA AGGACCCCAA TGGGAAGTGT GATCCTTACA TCAAGATCTC CATAGGGAAG 
AAATCAGTGA GTGACCAGGA TAACTACATT CCCTGTACTC TGGAGCCTGT GTTTGGAAAG 
ATGTTCGAGC TGACCTGCAC TCTGCCTCTG GAGAAGGACC TGAAGATCAC ACTCTATGAC 
TATGACCTCC TCTCCAAAGA TGAAAAGATT GGGGAGACAG TCATTGACCT GGAGGATAGG 
CTGCTGTCCA AGTTTGGGGC TCGCTGCGGA CTCCCACAGA CGTACTGTGT CTCTGGACCC 
AACCAGTGGA GGGACCAGCT CCGCCCCTCC CAGCTTCTCC ACCTCTTCTG CCAGCAGCGC 
AGACTCAAGG CTCCTGTGTA CCTCACGGAC CACGTGGTGT TCCAGGATAA AGAATACAGC 
ATTGAAGAAA TAGAGGCTGG CCGAGTCCCG AACCCACACC TGGGCCCAGT GGAAGAGCGT 
CTGGCCTTGC ATGTCCTCCA GCAGCAGGGC CTGGTCCCTG AGCATGTGGA GTCCCGGCCT 
CTCTACAGCC CCCTGCAGCC AGACATTGAA CAGGGGAAGC TGCAGATGTG GATCGACCTA 
TTTCCCAAGG CCCTGGGGCG TCCCGGACCT CCCTTCAACA TCACCCCGCG GAGAGCCAGA 
AGGTTTTTCC TGCGTTGTAT CATCTGGAAC ACAAAGGACG TGATCCTGGA CGACCTCAGC 
ATCACAGGGG AGAAGATGAG TGATATTTAT GTGAAGGGTT GGATGGTTGG CTTTGAAGAG 
CACAAGCAAA AGACAGATGT GCATTACCGG TCCCTGGGAG GTGAAGGCAA CTTTAACTGG 
AGGTTCATTT TCCCCTTTGA CTACCTGCCA GCCGAGCAAG TCTGCACCGT CTCTAAGAAG 
GATGCTTTCT GGAGGCTGGA CAAGACTGAG AGCAAAATCC CAGCACGAGT GGTATTTCAG 
ATCTGGGACA ATGACAAGTT CTCCTTTGAC GACTTTCTGG GCTCCTTGCA GTTGGATCTC 
AACCACATGC CCAAGCCTGC CAAGACAGCT GAGAAGTGCT CCTTGGACCA GCTGGATGAC 
ACTTTCCACC CGGAGTGGTT TGTGTCCCTT TTTGAACAGA AAACAGTTAA GGGCTGGTGG 
CCCTGCGTAG CAGAAGAGGG CGAGAAGAAG ATCCTGGCAG GCAAGCTGGA AATGACCTTG 
GAGATCGTAG CAGAGAGTGA GCATGAGGAG CGGCCTGCTG GCCAGGGCCG GGATGAACCC 
AACATGAACC CCAAGCTCGA GGACCCAAGG CGCCCTGACA CCTCCTTCCT CTGGTTCACC 
TCTCCATACA AGACTATGAA GTTCATCCTG TGGCGGCGGT TCCGATGTGC CATTATCCTC 
TTCATCATTC TCTTCATCCT GCTACTATTC TTAGGCATCT TCATCTATGC TTTCCCGAAC 
TACGCTGCCA TGAAGCTGGT GAAGCCCTTC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432224.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432272.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X19, mRNA.

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CloneID	OCe19215
Clone ID Related Accession (Same CDS sequence)	XM_003432224.4 , XM_003432224.3
Accession Version	XM_003432224.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432224.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC 
ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG 
GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG 
TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC 
CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG 
GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA 
GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA 
GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC 
TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT 
ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG 
CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA 
ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT 
GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC 
AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG 
AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC 
CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC 
GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC 
ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC 
TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT 
GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG 
ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT 
GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432272.1
CDS	405..6677
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432224.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432224.4

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC 
ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG 
GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG 
TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC 
CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG 
GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA 
GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA 
GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC 
TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT 
ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG 
CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA 
ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT 
GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC 
AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG 
AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC 
CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC 
GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC 
ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC 
TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT 
GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG 
ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT 
GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432224.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432272.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA.

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CloneID	OCe19215
Clone ID Related Accession (Same CDS sequence)	XM_003432224.4 , XM_003432224.3
Accession Version	XM_003432224.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6273bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432224.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC 
ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG 
GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG 
TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC 
CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG 
GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA 
GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA 
GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC 
TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT 
ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG 
CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA 
ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT 
GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC 
AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG 
AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC 
CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC 
GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC 
ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC 
TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT 
GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG 
ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT 
GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432272.1
CDS	439..6711
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQLADGLSGSAPTNMASSPSSPHEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432224.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432224.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC 
ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG 
GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG 
TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC 
CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG 
GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA 
GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA 
GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC 
TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT 
ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG 
CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA 
ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT 
GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC 
AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG 
AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC 
CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC 
GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC 
ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC 
TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT 
GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG 
ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGCTT 
GCAGATGGTC TGTCGGGCTC TGCCCCCACT AACATGGCAT CTTCTCCATC CAGTCCTCAT 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432227.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432275.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA.

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CloneID	OCe19216
Clone ID Related Accession (Same CDS sequence)	XM_003432227.3 , XM_003432227.4
Accession Version	XM_003432227.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC 
AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC 
CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC 
CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG 
AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC 
TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG 
AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG 
CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGGAGGAAGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA 
GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA 
GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG 
GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT 
GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC 
CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC 
CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA 
GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT 
CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT 
GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG 
CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC 
GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG 
TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG 
CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432275.1
CDS	51..6305
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432227.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432227.3

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC 
AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC 
CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC 
CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG 
AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC 
TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG 
AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG 
CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGGAGGAAGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA 
GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA 
GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG 
GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT 
GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC 
CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC 
CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA 
GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT 
CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT 
GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG 
CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC 
GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG 
TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG 
CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432227.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432275.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X20, mRNA.

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CloneID	OCe19216
Clone ID Related Accession (Same CDS sequence)	XM_003432227.3 , XM_003432227.4
Accession Version	XM_003432227.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6255bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432227.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC 
AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC 
CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC 
CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG 
AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC 
TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG 
AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG 
CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGGAGGAAGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA 
GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA 
GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG 
GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT 
GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC 
CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC 
CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA 
GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT 
CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT 
GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG 
CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC 
GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG 
TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG 
CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432275.1
CDS	23..6277
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432227.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432227.4

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGA GGAGCCTGCA 
GGTGTGGTCA AGCCTTCTCC AGCCACGGAC CTGGATGACC ATCTGGGCTT CCTTCCCACC 
TTCGGGCCCT GCTTCGTCAA TCTCTATGGC AGCCCCAGGG AATTCACTGG CTTCCCGGAC 
CCCTACGCGG AACTCAACAT GGGCAAGGGG GAAGGCGTGG CCTACCGAGG TCGGCTTCTG 
CTCTCCCTGG AGACCAAGCT GGTGGAGCAC AGCGGCGAGC AAAGGGTGGA GGACCTCCCT 
GCTGATGACA TCCTTCGGGT GGAGAAATAC CTCCGGAGGC GCAAGTACTC TCTCTTTGCC 
GCCTTCTACT CGGCCACCAT GCTCCAGGAT GTCGACGATG CCATCCAGTT TGAGGTCAGC 
ATTGGGAACT ATGGCAACAA GTTCGACACG ACCTGTCTGC CACTGGCCTC CACCACCCAG 
TACAGCCGGG CAGTCTTTGA TGGGTGCCAC TACTACTACT TGCCCTGGGG CAATGTGAAG 
CCTGTGGTGG TGCTGTCATC CTACTGGGAA GACATCAGCC ACAGAGTCGA GGCTCAGAAC 
CAGCTGCTCA GGATCGCTGA CCATCTGGAA GCTGGCCTGG AGCAAGTCCA CTTGGCTTTA 
AAGGCGCAGT TCAACACAGA GGATGTGGAC TCCCTGTTGG CCCAGCTGAT GGATGAGCTC 
ATCTCAGACT GCAGCCAGCC CCTGGGCAAT GTCCAAGAGA TACCCTCTGC CACCCACCTG 
GACCGGCACC TGTACAGGCT GCGCACCCGT CACCTAGGCC AGATTACTGA GGCGGCCCTG 
GCTCTGAAAC TCGGGCATGG CAAGCTCCCT GCCGCCCTGG AGCAGGCTGA AGACTGGCTC 
CTGCGTCTCC GTGCCCTGGC TGATGAGCCC CAGAACAGCC TGCCCGACAT CATCATCTGG 
ATGCTGCAGG GAAACAAGCG AGTGGCGTAC CAGCGGGTGC CTGCGCACGA GGTCCTCTTC 
TCCCGGCGGG GCGCCAGCTA CTGTGGCAAG AACTGTGGGA AGCTGCAGAC GATATTTCTG 
AAATATCCCA TGGAGGGGGT GCCCGGAGCC CGGATGCCAG TACAAATACG GGTGAAACTC 
TGGTTTGGGC TTTCTGTGGA TGAGAAGGAG TTCAACCAGT TTGCTGAGGG AAAGCTGTCC 
GTCTTTGCTG AAACTTATGA GAACCAGACC AAACTGGCCC TGGTTGGGAA CTGGGGCACA 
ACAGGCCTCA CCTACCCCAA GTTTTCTGAT GTCATGGGCA AGATCAAGCT ACCCAAGGAT 
AGCTTCCGCC CCTCAGCTGG CTGGACCTGG GCCGGAGACT GGTTCGTGTG TCCAGAGAAG 
ACCCTACTCC ATGACATGGA CGCGGGCCAC CTGAGTTTTG TGGAGGAGGT GTTTGAGAAC 
CAGACCCGGC TCCCTGGAGG CCAGTGGATC TACATGAGTG ACAACTACAC AGATGTGAAT 
GGGGATAAGG TGCTTCCTAA GGATGACATT GAGTGCCCAC TGGGCTGGAA GTGGGAAGAT 
GAAGAGTGGT CCACGGACCT CAATCGGGCC GTCGATGAGC AAGGCTGGGA GTACAGTATC 
ACCATCCCCC CGGATCGGAA GCCAAAGCAC TGGGTTCCTG CTGAGAAGAT GTATTACACG 
CACCGTCGGC GGCGCTGGGT CCGCCTGCGC AGAAGGGACC TCAGCCAGAT GGAGGCATTG 
AAGAGGCACA GACAGGCAGA GGCAGAGGGT GAGGGCTGGG AGTACGCCTC GCTCTTGGGC 
TGGAAGTTCC ACCTGGAGTA CCGCAAGACT GATGCTTTCC GCCGCCGCCG CTGGCGCCGC 
CGCATGGAAC CGCTGGAGAA GACGGGGCCT GCAGCCGTGT TTGCCCTTGA GGGGGCTCTG 
GGTGGTGTGG TAGATGACAG GAGTGAAGAT TCCGTGTCCG TGTCCACCTT GAGCTTTGGA 
GTGAACAGAC CCACGATTTC CTGCATCTTT GATTATGGGA ACCGCTACCA CCTACGCTGC 
TACATGTACC AGGCTCGGGA CCTGCCCTCG ATGGACAAGG ACTCTTTTTC TGATCCCTAC 
GCCATCGTCT CCTTCCTGCA CCAGAGCCAG AAGACAGTGG TGGCGAAGAA CACTCTGAAC 
CCCACCTGGG ACCAGACGCT TATCTTCTAT GAGATTGAGA TCTTTGGTGA GCCAGCCAGT 
ATCGCTGAGC AGCCGCCCAG CATTGTGGTG GAGCTGTATG ACCACGACAC CTACGGTGCA 
GATGAGTTTA TGGGACGCTG CATTTGTCAG CCCAGCCTGG AGCGGACACC CCGGCTGGCC 
TGGTTCCCAC TGATTAGGGG CAGCCAGTCG GCGGGGGAAC TGCTGGCCTC TTTTGAGCTC 
ATCCAGAGAG AAAAGCCGGC CATTCACCAT ATTCCTGGTT TTGAGGTACA GGATATGTCA 
GGAATCCTGG AAGAGTCAGA GGACATAGAC CTGCCCTACC CGCCACCTCA GAGGGAGGCC 
AACATCTTCA TGGTCCCCCA GAACATCAAG CCTGTTCTCC AGCGTACTGC CATAGAGATC 
CTGGCGTGGG GCCTGCGGAA CATGAAGAGC TACCAGCTAG CCAGCATCTC CTCCCCTAGC 
CTTGTGGTAG AGTGCGGGGG CCAGACAGTG CAGTCCTGTG TCATCAAGAA CCTCCGGAAG 
AACCCCAACT TCGACATCTG TACCCTTTTC ATGGAAGTGA TGCTGCCCAA GGAGGAGGTC 
TACTGTCCCT CCATCGTGAT CAAGGTCATT GATAACCGCC AGTTTGGCCG CCGGCCTGTG 
GTGGGCCAGT GTACCATCCG TTCCCTGGAG AGCTTCATGT GTGACCTGTA CTCCGAGGAG 
AATCCGTCCC CACAGGGCGG CCCAGATGAT GTGAGTTTAC TCAGTCCTGG GGAAGACGTG 
CTCATTGACA TCGATGACAA GGAGCCCCTC ATCCCCATGC AGGAGGAAGA GTTCATCGAC 
TGGTGGAGCA AATTCTTTGC CTCCACAGGG GAGAGGGAAA AGTGCGGCTC ATACTTGGAG 
AAGGGCTTTG ACACTCTAAA GGTCTATGAC ACACAACTGG AGAACGTGGA GGCCTTTGAG 
GGCCTGTCTG ACTTTTGTAA CACCTTCAAG CTCTACCGGG GCAAGACTCA GGAGGAGACA 
GAGGACCCAT CTGTTATTGG GGAGTTTAAG GGCCTCTTCA AAATCTATCC CCTCCCAGAA 
GACCCGGCTG TTCCCATGCC CCCGAGACAA TTCCACCAGC TGGCTGCCCA AGGGCCTCAG 
GAGTGCCTGG TCCGCATCTA TATCATCCGA GCATTTGGCC TTCAGCCCAA GGACCCCAAT 
GGGAAGTGTG ATCCTTACAT CAAGATCTCC ATAGGGAAGA AATCAGTGAG TGACCAGGAT 
AACTACATTC CCTGTACTCT GGAGCCTGTG TTTGGAAAGA TGTTCGAGCT GACCTGCACT 
CTGCCTCTGG AGAAGGACCT GAAGATCACA CTCTATGACT ATGACCTCCT CTCCAAAGAT 
GAAAAGATTG GGGAGACAGT CATTGACCTG GAGGATAGGC TGCTGTCCAA GTTTGGGGCT 
CGCTGCGGAC TCCCACAGAC GTACTGTGTC TCTGGACCCA ACCAGTGGAG GGACCAGCTC 
CGCCCCTCCC AGCTTCTCCA CCTCTTCTGC CAGCAGCGCA GACTCAAGGC TCCTGTGTAC 
CTCACGGACC ACGTGGTGTT CCAGGATAAA GAATACAGCA TTGAAGAAAT AGAGGCTGGC 
CGAGTCCCGA ACCCACACCT GGGCCCAGTG GAAGAGCGTC TGGCCTTGCA TGTCCTCCAG 
CAGCAGGGCC TGGTCCCTGA GCATGTGGAG TCCCGGCCTC TCTACAGCCC CCTGCAGCCA 
GACATTGAAC AGGGGAAGCT GCAGATGTGG ATCGACCTAT TTCCCAAGGC CCTGGGGCGT 
CCCGGACCTC CCTTCAACAT CACCCCGCGG AGAGCCAGAA GGTTTTTCCT GCGTTGTATC 
ATCTGGAACA CAAAGGACGT GATCCTGGAC GACCTCAGCA TCACAGGGGA GAAGATGAGT 
GATATTTATG TGAAGGGTTG GATGGTTGGC TTTGAAGAGC ACAAGCAAAA GACAGATGTG 
CATTACCGGT CCCTGGGAGG TGAAGGCAAC TTTAACTGGA GGTTCATTTT CCCCTTTGAC 
TACCTGCCAG CCGAGCAAGT CTGCACCGTC TCTAAGAAGG ATGCTTTCTG GAGGCTGGAC 
AAGACTGAGA GCAAAATCCC AGCACGAGTG GTATTTCAGA TCTGGGACAA TGACAAGTTC 
TCCTTTGACG ACTTTCTGGG CTCCTTGCAG TTGGATCTCA ACCACATGCC CAAGCCTGCC 
AAGACAGCTG AGAAGTGCTC CTTGGACCAG CTGGATGACA CTTTCCACCC GGAGTGGTTT 
GTGTCCCTTT TTGAACAGAA AACAGTTAAG GGCTGGTGGC CCTGCGTAGC AGAAGAGGGC 
GAGAAGAAGA TCCTGGCAGG CAAGCTGGAA ATGACCTTGG AGATCGTAGC AGAGAGTGAG 
CATGAGGAGC GGCCTGCTGG CCAGGGCCGG GATGAACCCA ACATGAACCC CAAGCTCGAG 
GACCCAAGGC GCCCTGACAC CTCCTTCCTC TGGTTCACCT CTCCATACAA GACTATGAAG 
TTCATCCTGT GGCGGCGGTT CCGATGTGCC ATTATCCTCT TCATCATTCT CTTCATCCTG 
CTACTATTCT TAGGCATCTT CATCTATGCT TTCCCGAACT ACGCTGCCAT GAAGCTGGTG 
AAGCCCTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_540237.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_540237.2 Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA.

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CloneID	OCe19217
Clone ID Related Accession (Same CDS sequence)	XM_540237.5 , XM_540237.6
Accession Version	XM_540237.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_540237.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC 
ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG 
GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT 
GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC 
TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGG AGGAAGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG 
GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC 
CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG 
TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA 
AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC 
ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA 
GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC 
ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC 
GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT 
ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT 
TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG 
AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT 
GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA 
CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_540237.2
CDS	438..6689
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_540237.5
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_540237.5

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC 
ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG 
GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT 
GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC 
TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGG AGGAAGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG 
GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC 
CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG 
TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA 
AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC 
ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA 
GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC 
ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC 
GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT 
ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT 
TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG 
AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT 
GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA 
CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_540237.6 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_540237.2 Canis lupus familiaris dysferlin (DYSF), transcript variant X21, mRNA.

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CloneID	OCe19217
Clone ID Related Accession (Same CDS sequence)	XM_540237.5 , XM_540237.6
Accession Version	XM_540237.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_540237.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC 
ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG 
GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT 
GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC 
TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGG AGGAAGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG 
GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC 
CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG 
TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA 
AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC 
ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA 
GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC 
ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC 
GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT 
ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT 
TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG 
AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT 
GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA 
CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_540237.2
CDS	405..6656
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEEEPAGVVKPSPATDLDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_540237.6
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_540237.6

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGAGGA GCCTGCAGGT 
GTGGTCAAGC CTTCTCCAGC CACGGACCTG GATGACCATC TGGGCTTCCT TCCCACCTTC 
GGGCCCTGCT TCGTCAATCT CTATGGCAGC CCCAGGGAAT TCACTGGCTT CCCGGACCCC 
TACGCGGAAC TCAACATGGG CAAGGGGGAA GGCGTGGCCT ACCGAGGTCG GCTTCTGCTC 
TCCCTGGAGA CCAAGCTGGT GGAGCACAGC GGCGAGCAAA GGGTGGAGGA CCTCCCTGCT 
GATGACATCC TTCGGGTGGA GAAATACCTC CGGAGGCGCA AGTACTCTCT CTTTGCCGCC 
TTCTACTCGG CCACCATGCT CCAGGATGTC GACGATGCCA TCCAGTTTGA GGTCAGCATT 
GGGAACTATG GCAACAAGTT CGACACGACC TGTCTGCCAC TGGCCTCCAC CACCCAGTAC 
AGCCGGGCAG TCTTTGATGG GTGCCACTAC TACTACTTGC CCTGGGGCAA TGTGAAGCCT 
GTGGTGGTGC TGTCATCCTA CTGGGAAGAC ATCAGCCACA GAGTCGAGGC TCAGAACCAG 
CTGCTCAGGA TCGCTGACCA TCTGGAAGCT GGCCTGGAGC AAGTCCACTT GGCTTTAAAG 
GCGCAGTTCA ACACAGAGGA TGTGGACTCC CTGTTGGCCC AGCTGATGGA TGAGCTCATC 
TCAGACTGCA GCCAGCCCCT GGGCAATGTC CAAGAGATAC CCTCTGCCAC CCACCTGGAC 
CGGCACCTGT ACAGGCTGCG CACCCGTCAC CTAGGCCAGA TTACTGAGGC GGCCCTGGCT 
CTGAAACTCG GGCATGGCAA GCTCCCTGCC GCCCTGGAGC AGGCTGAAGA CTGGCTCCTG 
CGTCTCCGTG CCCTGGCTGA TGAGCCCCAG AACAGCCTGC CCGACATCAT CATCTGGATG 
CTGCAGGGAA ACAAGCGAGT GGCGTACCAG CGGGTGCCTG CGCACGAGGT CCTCTTCTCC 
CGGCGGGGCG CCAGCTACTG TGGCAAGAAC TGTGGGAAGC TGCAGACGAT ATTTCTGAAA 
TATCCCATGG AGGGGGTGCC CGGAGCCCGG ATGCCAGTAC AAATACGGGT GAAACTCTGG 
TTTGGGCTTT CTGTGGATGA GAAGGAGTTC AACCAGTTTG CTGAGGGAAA GCTGTCCGTC 
TTTGCTGAAA CTTATGAGAA CCAGACCAAA CTGGCCCTGG TTGGGAACTG GGGCACAACA 
GGCCTCACCT ACCCCAAGTT TTCTGATGTC ATGGGCAAGA TCAAGCTACC CAAGGATAGC 
TTCCGCCCCT CAGCTGGCTG GACCTGGGCC GGAGACTGGT TCGTGTGTCC AGAGAAGACC 
CTACTCCATG ACATGGACGC GGGCCACCTG AGTTTTGTGG AGGAGGTGTT TGAGAACCAG 
ACCCGGCTCC CTGGAGGCCA GTGGATCTAC ATGAGTGACA ACTACACAGA TGTGAATGGG 
GATAAGGTGC TTCCTAAGGA TGACATTGAG TGCCCACTGG GCTGGAAGTG GGAAGATGAA 
GAGTGGTCCA CGGACCTCAA TCGGGCCGTC GATGAGCAAG GCTGGGAGTA CAGTATCACC 
ATCCCCCCGG ATCGGAAGCC AAAGCACTGG GTTCCTGCTG AGAAGATGTA TTACACGCAC 
CGTCGGCGGC GCTGGGTCCG CCTGCGCAGA AGGGACCTCA GCCAGATGGA GGCATTGAAG 
AGGCACAGAC AGGCAGAGGC AGAGGGTGAG GGCTGGGAGT ACGCCTCGCT CTTGGGCTGG 
AAGTTCCACC TGGAGTACCG CAAGACTGAT GCTTTCCGCC GCCGCCGCTG GCGCCGCCGC 
ATGGAACCGC TGGAGAAGAC GGGGCCTGCA GCCGTGTTTG CCCTTGAGGG GGCTCTGGGT 
GGTGTGGTAG ATGACAGGAG TGAAGATTCC GTGTCCGTGT CCACCTTGAG CTTTGGAGTG 
AACAGACCCA CGATTTCCTG CATCTTTGAT TATGGGAACC GCTACCACCT ACGCTGCTAC 
ATGTACCAGG CTCGGGACCT GCCCTCGATG GACAAGGACT CTTTTTCTGA TCCCTACGCC 
ATCGTCTCCT TCCTGCACCA GAGCCAGAAG ACAGTGGTGG CGAAGAACAC TCTGAACCCC 
ACCTGGGACC AGACGCTTAT CTTCTATGAG ATTGAGATCT TTGGTGAGCC AGCCAGTATC 
GCTGAGCAGC CGCCCAGCAT TGTGGTGGAG CTGTATGACC ACGACACCTA CGGTGCAGAT 
GAGTTTATGG GACGCTGCAT TTGTCAGCCC AGCCTGGAGC GGACACCCCG GCTGGCCTGG 
TTCCCACTGA TTAGGGGCAG CCAGTCGGCG GGGGAACTGC TGGCCTCTTT TGAGCTCATC 
CAGAGAGAAA AGCCGGCCAT TCACCATATT CCTGGTTTTG AGGTACAGGA TATGTCAGGA 
ATCCTGGAAG AGTCAGAGGA CATAGACCTG CCCTACCCGC CACCTCAGAG GGAGGCCAAC 
ATCTTCATGG TCCCCCAGAA CATCAAGCCT GTTCTCCAGC GTACTGCCAT AGAGATCCTG 
GCGTGGGGCC TGCGGAACAT GAAGAGCTAC CAGCTAGCCA GCATCTCCTC CCCTAGCCTT 
GTGGTAGAGT GCGGGGGCCA GACAGTGCAG TCCTGTGTCA TCAAGAACCT CCGGAAGAAC 
CCCAACTTCG ACATCTGTAC CCTTTTCATG GAAGTGATGC TGCCCAAGGA GGAGGTCTAC 
TGTCCCTCCA TCGTGATCAA GGTCATTGAT AACCGCCAGT TTGGCCGCCG GCCTGTGGTG 
GGCCAGTGTA CCATCCGTTC CCTGGAGAGC TTCATGTGTG ACCTGTACTC CGAGGAGAAT 
CCGTCCCCAC AGGGCGGCCC AGATGATGTG AGTTTACTCA GTCCTGGGGA AGACGTGCTC 
ATTGACATCG ATGACAAGGA GCCCCTCATC CCCATGCAGG AGGAAGAGTT CATCGACTGG 
TGGAGCAAAT TCTTTGCCTC CACAGGGGAG AGGGAAAAGT GCGGCTCATA CTTGGAGAAG 
GGCTTTGACA CTCTAAAGGT CTATGACACA CAACTGGAGA ACGTGGAGGC CTTTGAGGGC 
CTGTCTGACT TTTGTAACAC CTTCAAGCTC TACCGGGGCA AGACTCAGGA GGAGACAGAG 
GACCCATCTG TTATTGGGGA GTTTAAGGGC CTCTTCAAAA TCTATCCCCT CCCAGAAGAC 
CCGGCTGTTC CCATGCCCCC GAGACAATTC CACCAGCTGG CTGCCCAAGG GCCTCAGGAG 
TGCCTGGTCC GCATCTATAT CATCCGAGCA TTTGGCCTTC AGCCCAAGGA CCCCAATGGG 
AAGTGTGATC CTTACATCAA GATCTCCATA GGGAAGAAAT CAGTGAGTGA CCAGGATAAC 
TACATTCCCT GTACTCTGGA GCCTGTGTTT GGAAAGATGT TCGAGCTGAC CTGCACTCTG 
CCTCTGGAGA AGGACCTGAA GATCACACTC TATGACTATG ACCTCCTCTC CAAAGATGAA 
AAGATTGGGG AGACAGTCAT TGACCTGGAG GATAGGCTGC TGTCCAAGTT TGGGGCTCGC 
TGCGGACTCC CACAGACGTA CTGTGTCTCT GGACCCAACC AGTGGAGGGA CCAGCTCCGC 
CCCTCCCAGC TTCTCCACCT CTTCTGCCAG CAGCGCAGAC TCAAGGCTCC TGTGTACCTC 
ACGGACCACG TGGTGTTCCA GGATAAAGAA TACAGCATTG AAGAAATAGA GGCTGGCCGA 
GTCCCGAACC CACACCTGGG CCCAGTGGAA GAGCGTCTGG CCTTGCATGT CCTCCAGCAG 
CAGGGCCTGG TCCCTGAGCA TGTGGAGTCC CGGCCTCTCT ACAGCCCCCT GCAGCCAGAC 
ATTGAACAGG GGAAGCTGCA GATGTGGATC GACCTATTTC CCAAGGCCCT GGGGCGTCCC 
GGACCTCCCT TCAACATCAC CCCGCGGAGA GCCAGAAGGT TTTTCCTGCG TTGTATCATC 
TGGAACACAA AGGACGTGAT CCTGGACGAC CTCAGCATCA CAGGGGAGAA GATGAGTGAT 
ATTTATGTGA AGGGTTGGAT GGTTGGCTTT GAAGAGCACA AGCAAAAGAC AGATGTGCAT 
TACCGGTCCC TGGGAGGTGA AGGCAACTTT AACTGGAGGT TCATTTTCCC CTTTGACTAC 
CTGCCAGCCG AGCAAGTCTG CACCGTCTCT AAGAAGGATG CTTTCTGGAG GCTGGACAAG 
ACTGAGAGCA AAATCCCAGC ACGAGTGGTA TTTCAGATCT GGGACAATGA CAAGTTCTCC 
TTTGACGACT TTCTGGGCTC CTTGCAGTTG GATCTCAACC ACATGCCCAA GCCTGCCAAG 
ACAGCTGAGA AGTGCTCCTT GGACCAGCTG GATGACACTT TCCACCCGGA GTGGTTTGTG 
TCCCTTTTTG AACAGAAAAC AGTTAAGGGC TGGTGGCCCT GCGTAGCAGA AGAGGGCGAG 
AAGAAGATCC TGGCAGGCAA GCTGGAAATG ACCTTGGAGA TCGTAGCAGA GAGTGAGCAT 
GAGGAGCGGC CTGCTGGCCA GGGCCGGGAT GAACCCAACA TGAACCCCAA GCTCGAGGAC 
CCAAGGCGCC CTGACACCTC CTTCCTCTGG TTCACCTCTC CATACAAGAC TATGAAGTTC 
ATCCTGTGGC GGCGGTTCCG ATGTGCCATT ATCCTCTTCA TCATTCTCTT CATCCTGCTA 
CTATTCTTAG GCATCTTCAT CTATGCTTTC CCGAACTACG CTGCCATGAA GCTGGTGAAG 
CCCTTCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432232.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432280.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X23, mRNA.

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CloneID	OCe19218
Clone ID Related Accession (Same CDS sequence)	XM_003432232.4 , XM_003432232.3
Accession Version	XM_003432232.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432232.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA 
TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA 
AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC 
AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC 
ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA 
CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG 
CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC 
AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG 
CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC 
CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA 
CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG 
ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG 
CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG 
CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT 
GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG 
CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA 
CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT 
GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG 
GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG 
ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG 
TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG 
GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC 
AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG 
GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA 
GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT 
GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC 
AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG 
TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC 
CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT 
GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG 
TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC 
CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC 
TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG 
GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC 
TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC 
CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG 
CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG 
CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT 
GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG 
CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG 
CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC 
AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC 
ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG 
CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT 
GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC 
AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432280.1
CDS	22..6234
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432232.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432232.4

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA 
TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA 
AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC 
AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC 
ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA 
CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG 
CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC 
AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG 
CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC 
CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA 
CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG 
ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG 
CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG 
CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT 
GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG 
CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA 
CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT 
GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG 
GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG 
ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG 
TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG 
GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC 
AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG 
GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA 
GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT 
GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC 
AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG 
TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC 
CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT 
GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG 
TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC 
CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC 
TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG 
GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC 
TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC 
CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG 
CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG 
CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT 
GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG 
CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG 
CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC 
AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC 
ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG 
CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT 
GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC 
AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432232.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432280.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA.

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CloneID	OCe19218
Clone ID Related Accession (Same CDS sequence)	XM_003432232.4 , XM_003432232.3
Accession Version	XM_003432232.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432232.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA 
TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA 
AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC 
AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC 
ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA 
CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG 
CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC 
AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG 
CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC 
CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA 
CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG 
ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG 
CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG 
CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT 
GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG 
CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA 
CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT 
GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG 
GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG 
ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG 
TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG 
GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC 
AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG 
GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA 
GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT 
GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC 
AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG 
TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC 
CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT 
GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG 
TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC 
CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC 
TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG 
GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC 
TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC 
CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG 
CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG 
CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT 
GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG 
CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG 
CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC 
AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC 
ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG 
CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT 
GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC 
AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432280.1
CDS	50..6262
Translation	MLCCLLLRASNLPGGKKDRRSDPVASLTFRGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432232.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432232.3

ORF Insert Sequence:

ATGCTGTGCT GCCTGCTGCT GAGGGCCAGC AACCTCCCCG GCGGGAAGAA GGACCGGCGC 
AGCGACCCGG TCGCCAGCCT GACTTTCCGA GGAGTGAAGA AGAGAACCAA AGTCATCAAG 
AACAGCGTGA ACCCCGTGTG GAATGAGGGC TTTGAGTGGG ACCTCAAGGG CATCCCCTTG 
GACCAGAGCT CCGAGCTTCA TGTGGTGGTC AAAGACCATG AGACAATGGG GAGGAACAGG 
TTCTTAGGGG AAGCCCAAGT CCCACTCCAG GAGGTCCTCG CCACTCCTAG TCTGTCTGCC 
AGCTTCAATG CCCCCCTCCT GGACGCCAAG AAGCAGCCCA CGGGGGCCTC GCTGGTCCTG 
CAGGTGTCCT ACACTCCACT CCCTGGAGCC GTGCCCCTGT TCCCACCTGC TGCATCTCTG 
GAACCCTCCC CGACTCTGCC TGACATGGAT ATGGGGGCCG ACACAGGAGG AGAGGAGGAC 
ACTGAGGACC AGGGGCTCAC TGGAGACGAG GGAGAGCCAT TACTGGATCA GAGTGGCGTC 
CCAGGCCCTG GGGCTCCCAC CACCCCGAGG AAGCCACCTT CCCATCTGCC CCCCTACCAC 
CCTGGGAGCA AGAGGAAAAG AAGTGCTCCC GCAGCCAGAA AGCTGCTGTC CGACAAACCG 
CAGGACTTCC AGATCAGAGT GCAAGTGATC GAGGGCCGCC AGCTGCCAGG GGTGAATATC 
AAGCCTGTGA TCAAGGTCAC TGCTGCTGGG CAGACCAAGA GGACACGGAT CCACAAGGGA 
AACAGCCCAC TATTTAACGA GACTCTTTTC TTCAATGTGT TTGACTCTCC TTCGGAGCTG 
TTTGATGAGC CCATCTTTAT CACGGTCGTA GACTCCCGTT CTCTCAGGAC AGATGCTCTC 
ATTGGGGAAT TCCGGATGGA TGTGGGTACC ATCTACAGAG AGCCCCGACA TGCTTATCTG 
AGGAAGTGGC TGCTACTCTC CAACCCTGAT GACTTCTCTG TTGGGGCCAG AGGTTACCTG 
AAAGCAAGCC TTTATGTGCT GGGGCCCGGA GATGAAGCGC CTCTGGAGAG AAAGGACCCC 
TCTGAAGACA AGGAGGACAT TGAAAACAAT CTGCTCAGGC CAACTGGCGT GGCCCTGCGA 
GGAGCCCACT TCTGCCTGAA GATCTTCAGA GCGGAGGACT TACCACAAAT GGATGATGCC 
GTGATGGACA ATGTGAAGCA GATCTTTGGC TTTGACAGCA ACAAGAAGAA TTTGGTAGAT 
CCCTTCATGG AAGTCAGCTT TGCGGGGAAA ATGCTCTGCA GCAAGATCCT GGAGAAGACA 
GCCAACCCTC AGTGGAACCA GAGCATCACT CTGCCTGCCA TGTTTCCCTC CATGTGTGAA 
AAAATGAAGA TTCGCGTCAT GGACTGGGAC CGCCTTACCC ACAATGACAT CGTGGCCACC 
ACCTACCTGA GTATGTCGAA AATCTCTGCC CCTGGAGGAG AAATAGAAGT GGATGACCAT 
CTGGGCTTCC TTCCCACCTT CGGGCCCTGC TTCGTCAATC TCTATGGCAG CCCCAGGGAA 
TTCACTGGCT TCCCGGACCC CTACGCGGAA CTCAACATGG GCAAGGGGGA AGGCGTGGCC 
TACCGAGGTC GGCTTCTGCT CTCCCTGGAG ACCAAGCTGG TGGAGCACAG CGGCGAGCAA 
AGGGTGGAGG ACCTCCCTGC TGATGACATC CTTCGGGTGG AGAAATACCT CCGGAGGCGC 
AAGTACTCTC TCTTTGCCGC CTTCTACTCG GCCACCATGC TCCAGGATGT CGACGATGCC 
ATCCAGTTTG AGGTCAGCAT TGGGAACTAT GGCAACAAGT TCGACACGAC CTGTCTGCCA 
CTGGCCTCCA CCACCCAGTA CAGCCGGGCA GTCTTTGATG GGTGCCACTA CTACTACTTG 
CCCTGGGGCA ATGTGAAGCC TGTGGTGGTG CTGTCATCCT ACTGGGAAGA CATCAGCCAC 
AGAGTCGAGG CTCAGAACCA GCTGCTCAGG ATCGCTGACC ATCTGGAAGC TGGCCTGGAG 
CAAGTCCACT TGGCTTTAAA GGCGCAGTTC AACACAGAGG ATGTGGACTC CCTGTTGGCC 
CAGCTGATGG ATGAGCTCAT CTCAGACTGC AGCCAGCCCC TGGGCAATGT CCAAGAGATA 
CCCTCTGCCA CCCACCTGGA CCGGCACCTG TACAGGCTGC GCACCCGTCA CCTAGGCCAG 
ATTACTGAGG CGGCCCTGGC TCTGAAACTC GGGCATGGCA AGCTCCCTGC CGCCCTGGAG 
CAGGCTGAAG ACTGGCTCCT GCGTCTCCGT GCCCTGGCTG ATGAGCCCCA GAACAGCCTG 
CCCGACATCA TCATCTGGAT GCTGCAGGGA AACAAGCGAG TGGCGTACCA GCGGGTGCCT 
GCGCACGAGG TCCTCTTCTC CCGGCGGGGC GCCAGCTACT GTGGCAAGAA CTGTGGGAAG 
CTGCAGACGA TATTTCTGAA ATATCCCATG GAGGGGGTGC CCGGAGCCCG GATGCCAGTA 
CAAATACGGG TGAAACTCTG GTTTGGGCTT TCTGTGGATG AGAAGGAGTT CAACCAGTTT 
GCTGAGGGAA AGCTGTCCGT CTTTGCTGAA ACTTATGAGA ACCAGACCAA ACTGGCCCTG 
GTTGGGAACT GGGGCACAAC AGGCCTCACC TACCCCAAGT TTTCTGATGT CATGGGCAAG 
ATCAAGCTAC CCAAGGATAG CTTCCGCCCC TCAGCTGGCT GGACCTGGGC CGGAGACTGG 
TTCGTGTGTC CAGAGAAGAC CCTACTCCAT GACATGGACG CGGGCCACCT GAGTTTTGTG 
GAGGAGGTGT TTGAGAACCA GACCCGGCTC CCTGGAGGCC AGTGGATCTA CATGAGTGAC 
AACTACACAG ATGTGAATGG GGATAAGGTG CTTCCTAAGG ATGACATTGA GTGCCCACTG 
GGCTGGAAGT GGGAAGATGA AGAGTGGTCC ACGGACCTCA ATCGGGCCGT CGATGAGCAA 
GGCTGGGAGT ACAGTATCAC CATCCCCCCG GATCGGAAGC CAAAGCACTG GGTTCCTGCT 
GAGAAGATGT ATTACACGCA CCGTCGGCGG CGCTGGGTCC GCCTGCGCAG AAGGGACCTC 
AGCCAGATGG AGGCATTGAA GAGGCACAGA CAGGCAGAGG CAGAGGGTGA GGGCTGGGAG 
TACGCCTCGC TCTTGGGCTG GAAGTTCCAC CTGGAGTACC GCAAGACTGA TGCTTTCCGC 
CGCCGCCGCT GGCGCCGCCG CATGGAACCG CTGGAGAAGA CGGGGCCTGC AGCCGTGTTT 
GCCCTTGAGG GGGCTCTGGG TGGTGTGGTA GATGACAGGA GTGAAGATTC CGTGTCCGTG 
TCCACCTTGA GCTTTGGAGT GAACAGACCC ACGATTTCCT GCATCTTTGA TTATGGGAAC 
CGCTACCACC TACGCTGCTA CATGTACCAG GCTCGGGACC TGCCCTCGAT GGACAAGGAC 
TCTTTTTCTG ATCCCTACGC CATCGTCTCC TTCCTGCACC AGAGCCAGAA GACAGTGGTG 
GCGAAGAACA CTCTGAACCC CACCTGGGAC CAGACGCTTA TCTTCTATGA GATTGAGATC 
TTTGGTGAGC CAGCCAGTAT CGCTGAGCAG CCGCCCAGCA TTGTGGTGGA GCTGTATGAC 
CACGACACCT ACGGTGCAGA TGAGTTTATG GGACGCTGCA TTTGTCAGCC CAGCCTGGAG 
CGGACACCCC GGCTGGCCTG GTTCCCACTG ATTAGGGGCA GCCAGTCGGC GGGGGAACTG 
CTGGCCTCTT TTGAGCTCAT CCAGAGAGAA AAGCCGGCCA TTCACCATAT TCCTGGTTTT 
GAGGTACAGG ATATGTCAGG AATCCTGGAA GAGTCAGAGG ACATAGACCT GCCCTACCCG 
CCACCTCAGA GGGAGGCCAA CATCTTCATG GTCCCCCAGA ACATCAAGCC TGTTCTCCAG 
CGTACTGCCA TAGAGATCCT GGCGTGGGGC CTGCGGAACA TGAAGAGCTA CCAGCTAGCC 
AGCATCTCCT CCCCTAGCCT TGTGGTAGAG TGCGGGGGCC AGACAGTGCA GTCCTGTGTC 
ATCAAGAACC TCCGGAAGAA CCCCAACTTC GACATCTGTA CCCTTTTCAT GGAAGTGATG 
CTGCCCAAGG AGGAGGTCTA CTGTCCCTCC ATCGTGATCA AGGTCATTGA TAACCGCCAG 
TTTGGCCGCC GGCCTGTGGT GGGCCAGTGT ACCATCCGTT CCCTGGAGAG CTTCATGTGT 
GACCTGTACT CCGAGGAGAA TCCGTCCCCA CAGGGCGGCC CAGATGATGT GAGTTTACTC 
AGTCCTGGGG AAGACGTGCT CATTGACATC GATGACAAGG AGCCCCTCAT CCCCATGCAG 
GAGGAAGAGT TCATCGACTG GTGGAGCAAA TTCTTTGCCT CCACAGGGGA GAGGGAAAAG 
TGCGGCTCAT ACTTGGAGAA GGGCTTTGAC ACTCTAAAGG TCTATGACAC ACAACTGGAG 
AACGTGGAGG CCTTTGAGGG CCTGTCTGAC TTTTGTAACA CCTTCAAGCT CTACCGGGGC 
AAGACTCAGG AGGAGACAGA GGACCCATCT GTTATTGGGG AGTTTAAGGG CCTCTTCAAA 
ATCTATCCCC TCCCAGAAGA CCCGGCTGTT CCCATGCCCC CGAGACAATT CCACCAGCTG 
GCTGCCCAAG GGCCTCAGGA GTGCCTGGTC CGCATCTATA TCATCCGAGC ATTTGGCCTT 
CAGCCCAAGG ACCCCAATGG GAAGTGTGAT CCTTACATCA AGATCTCCAT AGGGAAGAAA 
TCAGTGAGTG ACCAGGATAA CTACATTCCC TGTACTCTGG AGCCTGTGTT TGGAAAGATG 
TTCGAGCTGA CCTGCACTCT GCCTCTGGAG AAGGACCTGA AGATCACACT CTATGACTAT 
GACCTCCTCT CCAAAGATGA AAAGATTGGG GAGACAGTCA TTGACCTGGA GGATAGGCTG 
CTGTCCAAGT TTGGGGCTCG CTGCGGACTC CCACAGACGT ACTGTGTCTC TGGACCCAAC 
CAGTGGAGGG ACCAGCTCCG CCCCTCCCAG CTTCTCCACC TCTTCTGCCA GCAGCGCAGA 
CTCAAGGCTC CTGTGTACCT CACGGACCAC GTGGTGTTCC AGGATAAAGA ATACAGCATT 
GAAGAAATAG AGGCTGGCCG AGTCCCGAAC CCACACCTGG GCCCAGTGGA AGAGCGTCTG 
GCCTTGCATG TCCTCCAGCA GCAGGGCCTG GTCCCTGAGC ATGTGGAGTC CCGGCCTCTC 
TACAGCCCCC TGCAGCCAGA CATTGAACAG GGGAAGCTGC AGATGTGGAT CGACCTATTT 
CCCAAGGCCC TGGGGCGTCC CGGACCTCCC TTCAACATCA CCCCGCGGAG AGCCAGAAGG 
TTTTTCCTGC GTTGTATCAT CTGGAACACA AAGGACGTGA TCCTGGACGA CCTCAGCATC 
ACAGGGGAGA AGATGAGTGA TATTTATGTG AAGGGTTGGA TGGTTGGCTT TGAAGAGCAC 
AAGCAAAAGA CAGATGTGCA TTACCGGTCC CTGGGAGGTG AAGGCAACTT TAACTGGAGG 
TTCATTTTCC CCTTTGACTA CCTGCCAGCC GAGCAAGTCT GCACCGTCTC TAAGAAGGAT 
GCTTTCTGGA GGCTGGACAA GACTGAGAGC AAAATCCCAG CACGAGTGGT ATTTCAGATC 
TGGGACAATG ACAAGTTCTC CTTTGACGAC TTTCTGGGCT CCTTGCAGTT GGATCTCAAC 
CACATGCCCA AGCCTGCCAA GACAGCTGAG AAGTGCTCCT TGGACCAGCT GGATGACACT 
TTCCACCCGG AGTGGTTTGT GTCCCTTTTT GAACAGAAAA CAGTTAAGGG CTGGTGGCCC 
TGCGTAGCAG AAGAGGGCGA GAAGAAGATC CTGGCAGGCA AGCTGGAAAT GACCTTGGAG 
ATCGTAGCAG AGAGTGAGCA TGAGGAGCGG CCTGCTGGCC AGGGCCGGGA TGAACCCAAC 
ATGAACCCCA AGCTCGAGGA CCCAAGGCGC CCTGACACCT CCTTCCTCTG GTTCACCTCT 
CCATACAAGA CTATGAAGTT CATCCTGTGG CGGCGGTTCC GATGTGCCAT TATCCTCTTC 
ATCATTCTCT TCATCCTGCT ACTATTCTTA GGCATCTTCA TCTATGCTTT CCCGAACTAC 
GCTGCCATGA AGCTGGTGAA GCCCTTCAGC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432223.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432271.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA.

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CloneID	OCe19219
Clone ID Related Accession (Same CDS sequence)	XM_003432223.3 , XM_003432223.4
Accession Version	XM_003432223.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	dysferlin isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_003432223.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC 
ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG 
GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG 
TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC 
CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG 
GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA 
GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA 
GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC 
TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT 
ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG 
CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA 
ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT 
GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC 
AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG 
AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC 
CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC 
GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC 
ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC 
TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT 
GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG 
ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG 
GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC 
GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC 
TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT 
GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC 
CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC 
AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA 
GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC 
AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA 
GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG 
CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC 
GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA 
TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC 
ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432271.1
CDS	438..6647
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432223.3
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432223.3

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC 
ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG 
GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG 
TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC 
CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG 
GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA 
GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA 
GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC 
TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT 
ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG 
CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA 
ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT 
GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC 
AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG 
AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC 
CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC 
GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC 
ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC 
TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT 
GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG 
ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG 
GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC 
GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC 
TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT 
GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC 
CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC 
AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA 
GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC 
AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA 
GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG 
CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC 
GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA 
TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC 
ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DYSF ( XM_003432223.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_003432271.1 Canis lupus familiaris dysferlin (DYSF), transcript variant X24, mRNA.

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CloneID	OCe19219
Clone ID Related Accession (Same CDS sequence)	XM_003432223.3 , XM_003432223.4
Accession Version	XM_003432223.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	dysferlin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006599.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_003432223.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC 
ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG 
GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG 
TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC 
CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG 
GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA 
GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA 
GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC 
TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT 
ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG 
CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA 
ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT 
GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC 
AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG 
AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC 
CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC 
GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC 
ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC 
TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT 
GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG 
ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG 
GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC 
GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC 
TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT 
GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC 
CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC 
AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA 
GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC 
AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA 
GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG 
CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC 
GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA 
TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC 
ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003432271.1
CDS	405..6614
Translation	MLRVFILYAENVHTPDADISDAYCSAVFAGVKKRTKVIKNSVNPVWNEGFEWDLKGIPLDQSSELHVVVKDHETMGRNRFLGEAQVPLQEVLATPSLSASFNAPLLDAKKQPTGASLVLQVSYTPLPGAVPLFPPAASLEPSPTLPDMDMGADTGGEEDTEDQGLTGDEGEPLLDQSGVPGPGAPTTPRKPPSHLPPYHPGSKRKRSAPAARKLLSDKPQDFQIRVQVIEGRQLPGVNIKPVIKVTAAGQTKRTRIHKGNSPLFNETLFFNVFDSPSELFDEPIFITVVDSRSLRTDALIGEFRMDVGTIYREPRHAYLRKWLLLSNPDDFSVGARGYLKASLYVLGPGDEAPLERKDPSEDKEDIENNLLRPTGVALRGAHFCLKIFRAEDLPQMDDAVMDNVKQIFGFDSNKKNLVDPFMEVSFAGKMLCSKILEKTANPQWNQSITLPAMFPSMCEKMKIRVMDWDRLTHNDIVATTYLSMSKISAPGGEIEVDDHLGFLPTFGPCFVNLYGSPREFTGFPDPYAELNMGKGEGVAYRGRLLLSLETKLVEHSGEQRVEDLPADDILRVEKYLRRRKYSLFAAFYSATMLQDVDDAIQFEVSIGNYGNKFDTTCLPLASTTQYSRAVFDGCHYYYLPWGNVKPVVVLSSYWEDISHRVEAQNQLLRIADHLEAGLEQVHLALKAQFNTEDVDSLLAQLMDELISDCSQPLGNVQEIPSATHLDRHLYRLRTRHLGQITEAALALKLGHGKLPAALEQAEDWLLRLRALADEPQNSLPDIIIWMLQGNKRVAYQRVPAHEVLFSRRGASYCGKNCGKLQTIFLKYPMEGVPGARMPVQIRVKLWFGLSVDEKEFNQFAEGKLSVFAETYENQTKLALVGNWGTTGLTYPKFSDVMGKIKLPKDSFRPSAGWTWAGDWFVCPEKTLLHDMDAGHLSFVEEVFENQTRLPGGQWIYMSDNYTDVNGDKVLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPDRKPKHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLLGWKFHLEYRKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVVDDRSEDSVSVSTLSFGVNRPTISCIFDYGNRYHLRCYMYQARDLPSMDKDSFSDPYAIVSFLHQSQKTVVAKNTLNPTWDQTLIFYEIEIFGEPASIAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERTPRLAWFPLIRGSQSAGELLASFELIQREKPAIHHIPGFEVQDMSGILEESEDIDLPYPPPQREANIFMVPQNIKPVLQRTAIEILAWGLRNMKSYQLASISSPSLVVECGGQTVQSCVIKNLRKNPNFDICTLFMEVMLPKEEVYCPSIVIKVIDNRQFGRRPVVGQCTIRSLESFMCDLYSEENPSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPMQEEEFIDWWSKFFASTGEREKCGSYLEKGFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLPEDPAVPMPPRQFHQLAAQGPQECLVRIYIIRAFGLQPKDPNGKCDPYIKISIGKKSVSDQDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVIDLEDRLLSKFGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQRRLKAPVYLTDHVVFQDKEYSIEEIEAGRVPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWIDLFPKALGRPGPPFNITPRRARRFFLRCIIWNTKDVILDDLSITGEKMSDIYVKGWMVGFEEHKQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTVSKKDAFWRLDKTESKIPARVVFQIWDNDKFSFDDFLGSLQLDLNHMPKPAKTAEKCSLDQLDDTFHPEWFVSLFEQKTVKGWWPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLWFTSPYKTMKFILWRRFRCAIILFIILFILLLFLGIFIYAFPNYAAMKLVKPFS

Target ORF information:

RefSeq Version	XM_003432223.4
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris dysferlin (DYSF), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003432223.4

ORF Insert Sequence:

ATGCTGAGGG TCTTCATCCT CTACGCCGAG AATGTCCACA CCCCGGACGC GGACATCAGC 
GATGCCTACT GCTCCGCGGT GTTTGCAGGA GTGAAGAAGA GAACCAAAGT CATCAAGAAC 
AGCGTGAACC CCGTGTGGAA TGAGGGCTTT GAGTGGGACC TCAAGGGCAT CCCCTTGGAC 
CAGAGCTCCG AGCTTCATGT GGTGGTCAAA GACCATGAGA CAATGGGGAG GAACAGGTTC 
TTAGGGGAAG CCCAAGTCCC ACTCCAGGAG GTCCTCGCCA CTCCTAGTCT GTCTGCCAGC 
TTCAATGCCC CCCTCCTGGA CGCCAAGAAG CAGCCCACGG GGGCCTCGCT GGTCCTGCAG 
GTGTCCTACA CTCCACTCCC TGGAGCCGTG CCCCTGTTCC CACCTGCTGC ATCTCTGGAA 
CCCTCCCCGA CTCTGCCTGA CATGGATATG GGGGCCGACA CAGGAGGAGA GGAGGACACT 
GAGGACCAGG GGCTCACTGG AGACGAGGGA GAGCCATTAC TGGATCAGAG TGGCGTCCCA 
GGCCCTGGGG CTCCCACCAC CCCGAGGAAG CCACCTTCCC ATCTGCCCCC CTACCACCCT 
GGGAGCAAGA GGAAAAGAAG TGCTCCCGCA GCCAGAAAGC TGCTGTCCGA CAAACCGCAG 
GACTTCCAGA TCAGAGTGCA AGTGATCGAG GGCCGCCAGC TGCCAGGGGT GAATATCAAG 
CCTGTGATCA AGGTCACTGC TGCTGGGCAG ACCAAGAGGA CACGGATCCA CAAGGGAAAC 
AGCCCACTAT TTAACGAGAC TCTTTTCTTC AATGTGTTTG ACTCTCCTTC GGAGCTGTTT 
GATGAGCCCA TCTTTATCAC GGTCGTAGAC TCCCGTTCTC TCAGGACAGA TGCTCTCATT 
GGGGAATTCC GGATGGATGT GGGTACCATC TACAGAGAGC CCCGACATGC TTATCTGAGG 
AAGTGGCTGC TACTCTCCAA CCCTGATGAC TTCTCTGTTG GGGCCAGAGG TTACCTGAAA 
GCAAGCCTTT ATGTGCTGGG GCCCGGAGAT GAAGCGCCTC TGGAGAGAAA GGACCCCTCT 
GAAGACAAGG AGGACATTGA AAACAATCTG CTCAGGCCAA CTGGCGTGGC CCTGCGAGGA 
GCCCACTTCT GCCTGAAGAT CTTCAGAGCG GAGGACTTAC CACAAATGGA TGATGCCGTG 
ATGGACAATG TGAAGCAGAT CTTTGGCTTT GACAGCAACA AGAAGAATTT GGTAGATCCC 
TTCATGGAAG TCAGCTTTGC GGGGAAAATG CTCTGCAGCA AGATCCTGGA GAAGACAGCC 
AACCCTCAGT GGAACCAGAG CATCACTCTG CCTGCCATGT TTCCCTCCAT GTGTGAAAAA 
ATGAAGATTC GCGTCATGGA CTGGGACCGC CTTACCCACA ATGACATCGT GGCCACCACC 
TACCTGAGTA TGTCGAAAAT CTCTGCCCCT GGAGGAGAAA TAGAAGTGGA TGACCATCTG 
GGCTTCCTTC CCACCTTCGG GCCCTGCTTC GTCAATCTCT ATGGCAGCCC CAGGGAATTC 
ACTGGCTTCC CGGACCCCTA CGCGGAACTC AACATGGGCA AGGGGGAAGG CGTGGCCTAC 
CGAGGTCGGC TTCTGCTCTC CCTGGAGACC AAGCTGGTGG AGCACAGCGG CGAGCAAAGG 
GTGGAGGACC TCCCTGCTGA TGACATCCTT CGGGTGGAGA AATACCTCCG GAGGCGCAAG 
TACTCTCTCT TTGCCGCCTT CTACTCGGCC ACCATGCTCC AGGATGTCGA CGATGCCATC 
CAGTTTGAGG TCAGCATTGG GAACTATGGC AACAAGTTCG ACACGACCTG TCTGCCACTG 
GCCTCCACCA CCCAGTACAG CCGGGCAGTC TTTGATGGGT GCCACTACTA CTACTTGCCC 
TGGGGCAATG TGAAGCCTGT GGTGGTGCTG TCATCCTACT GGGAAGACAT CAGCCACAGA 
GTCGAGGCTC AGAACCAGCT GCTCAGGATC GCTGACCATC TGGAAGCTGG CCTGGAGCAA 
GTCCACTTGG CTTTAAAGGC GCAGTTCAAC ACAGAGGATG TGGACTCCCT GTTGGCCCAG 
CTGATGGATG AGCTCATCTC AGACTGCAGC CAGCCCCTGG GCAATGTCCA AGAGATACCC 
TCTGCCACCC ACCTGGACCG GCACCTGTAC AGGCTGCGCA CCCGTCACCT AGGCCAGATT 
ACTGAGGCGG CCCTGGCTCT GAAACTCGGG CATGGCAAGC TCCCTGCCGC CCTGGAGCAG 
GCTGAAGACT GGCTCCTGCG TCTCCGTGCC CTGGCTGATG AGCCCCAGAA CAGCCTGCCC 
GACATCATCA TCTGGATGCT GCAGGGAAAC AAGCGAGTGG CGTACCAGCG GGTGCCTGCG 
CACGAGGTCC TCTTCTCCCG GCGGGGCGCC AGCTACTGTG GCAAGAACTG TGGGAAGCTG 
CAGACGATAT TTCTGAAATA TCCCATGGAG GGGGTGCCCG GAGCCCGGAT GCCAGTACAA 
ATACGGGTGA AACTCTGGTT TGGGCTTTCT GTGGATGAGA AGGAGTTCAA CCAGTTTGCT 
GAGGGAAAGC TGTCCGTCTT TGCTGAAACT TATGAGAACC AGACCAAACT GGCCCTGGTT 
GGGAACTGGG GCACAACAGG CCTCACCTAC CCCAAGTTTT CTGATGTCAT GGGCAAGATC 
AAGCTACCCA AGGATAGCTT CCGCCCCTCA GCTGGCTGGA CCTGGGCCGG AGACTGGTTC 
GTGTGTCCAG AGAAGACCCT ACTCCATGAC ATGGACGCGG GCCACCTGAG TTTTGTGGAG 
GAGGTGTTTG AGAACCAGAC CCGGCTCCCT GGAGGCCAGT GGATCTACAT GAGTGACAAC 
TACACAGATG TGAATGGGGA TAAGGTGCTT CCTAAGGATG ACATTGAGTG CCCACTGGGC 
TGGAAGTGGG AAGATGAAGA GTGGTCCACG GACCTCAATC GGGCCGTCGA TGAGCAAGGC 
TGGGAGTACA GTATCACCAT CCCCCCGGAT CGGAAGCCAA AGCACTGGGT TCCTGCTGAG 
AAGATGTATT ACACGCACCG TCGGCGGCGC TGGGTCCGCC TGCGCAGAAG GGACCTCAGC 
CAGATGGAGG CATTGAAGAG GCACAGACAG GCAGAGGCAG AGGGTGAGGG CTGGGAGTAC 
GCCTCGCTCT TGGGCTGGAA GTTCCACCTG GAGTACCGCA AGACTGATGC TTTCCGCCGC 
CGCCGCTGGC GCCGCCGCAT GGAACCGCTG GAGAAGACGG GGCCTGCAGC CGTGTTTGCC 
CTTGAGGGGG CTCTGGGTGG TGTGGTAGAT GACAGGAGTG AAGATTCCGT GTCCGTGTCC 
ACCTTGAGCT TTGGAGTGAA CAGACCCACG ATTTCCTGCA TCTTTGATTA TGGGAACCGC 
TACCACCTAC GCTGCTACAT GTACCAGGCT CGGGACCTGC CCTCGATGGA CAAGGACTCT 
TTTTCTGATC CCTACGCCAT CGTCTCCTTC CTGCACCAGA GCCAGAAGAC AGTGGTGGCG 
AAGAACACTC TGAACCCCAC CTGGGACCAG ACGCTTATCT TCTATGAGAT TGAGATCTTT 
GGTGAGCCAG CCAGTATCGC TGAGCAGCCG CCCAGCATTG TGGTGGAGCT GTATGACCAC 
GACACCTACG GTGCAGATGA GTTTATGGGA CGCTGCATTT GTCAGCCCAG CCTGGAGCGG 
ACACCCCGGC TGGCCTGGTT CCCACTGATT AGGGGCAGCC AGTCGGCGGG GGAACTGCTG 
GCCTCTTTTG AGCTCATCCA GAGAGAAAAG CCGGCCATTC ACCATATTCC TGGTTTTGAG 
GTACAGGATA TGTCAGGAAT CCTGGAAGAG TCAGAGGACA TAGACCTGCC CTACCCGCCA 
CCTCAGAGGG AGGCCAACAT CTTCATGGTC CCCCAGAACA TCAAGCCTGT TCTCCAGCGT 
ACTGCCATAG AGATCCTGGC GTGGGGCCTG CGGAACATGA AGAGCTACCA GCTAGCCAGC 
ATCTCCTCCC CTAGCCTTGT GGTAGAGTGC GGGGGCCAGA CAGTGCAGTC CTGTGTCATC 
AAGAACCTCC GGAAGAACCC CAACTTCGAC ATCTGTACCC TTTTCATGGA AGTGATGCTG 
CCCAAGGAGG AGGTCTACTG TCCCTCCATC GTGATCAAGG TCATTGATAA CCGCCAGTTT 
GGCCGCCGGC CTGTGGTGGG CCAGTGTACC ATCCGTTCCC TGGAGAGCTT CATGTGTGAC 
CTGTACTCCG AGGAGAATCC GTCCCCACAG GGCGGCCCAG ATGATGTGAG TTTACTCAGT 
CCTGGGGAAG ACGTGCTCAT TGACATCGAT GACAAGGAGC CCCTCATCCC CATGCAGGAG 
GAAGAGTTCA TCGACTGGTG GAGCAAATTC TTTGCCTCCA CAGGGGAGAG GGAAAAGTGC 
GGCTCATACT TGGAGAAGGG CTTTGACACT CTAAAGGTCT ATGACACACA ACTGGAGAAC 
GTGGAGGCCT TTGAGGGCCT GTCTGACTTT TGTAACACCT TCAAGCTCTA CCGGGGCAAG 
ACTCAGGAGG AGACAGAGGA CCCATCTGTT ATTGGGGAGT TTAAGGGCCT CTTCAAAATC 
TATCCCCTCC CAGAAGACCC GGCTGTTCCC ATGCCCCCGA GACAATTCCA CCAGCTGGCT 
GCCCAAGGGC CTCAGGAGTG CCTGGTCCGC ATCTATATCA TCCGAGCATT TGGCCTTCAG 
CCCAAGGACC CCAATGGGAA GTGTGATCCT TACATCAAGA TCTCCATAGG GAAGAAATCA 
GTGAGTGACC AGGATAACTA CATTCCCTGT ACTCTGGAGC CTGTGTTTGG AAAGATGTTC 
GAGCTGACCT GCACTCTGCC TCTGGAGAAG GACCTGAAGA TCACACTCTA TGACTATGAC 
CTCCTCTCCA AAGATGAAAA GATTGGGGAG ACAGTCATTG ACCTGGAGGA TAGGCTGCTG 
TCCAAGTTTG GGGCTCGCTG CGGACTCCCA CAGACGTACT GTGTCTCTGG ACCCAACCAG 
TGGAGGGACC AGCTCCGCCC CTCCCAGCTT CTCCACCTCT TCTGCCAGCA GCGCAGACTC 
AAGGCTCCTG TGTACCTCAC GGACCACGTG GTGTTCCAGG ATAAAGAATA CAGCATTGAA 
GAAATAGAGG CTGGCCGAGT CCCGAACCCA CACCTGGGCC CAGTGGAAGA GCGTCTGGCC 
TTGCATGTCC TCCAGCAGCA GGGCCTGGTC CCTGAGCATG TGGAGTCCCG GCCTCTCTAC 
AGCCCCCTGC AGCCAGACAT TGAACAGGGG AAGCTGCAGA TGTGGATCGA CCTATTTCCC 
AAGGCCCTGG GGCGTCCCGG ACCTCCCTTC AACATCACCC CGCGGAGAGC CAGAAGGTTT 
TTCCTGCGTT GTATCATCTG GAACACAAAG GACGTGATCC TGGACGACCT CAGCATCACA 
GGGGAGAAGA TGAGTGATAT TTATGTGAAG GGTTGGATGG TTGGCTTTGA AGAGCACAAG 
CAAAAGACAG ATGTGCATTA CCGGTCCCTG GGAGGTGAAG GCAACTTTAA CTGGAGGTTC 
ATTTTCCCCT TTGACTACCT GCCAGCCGAG CAAGTCTGCA CCGTCTCTAA GAAGGATGCT 
TTCTGGAGGC TGGACAAGAC TGAGAGCAAA ATCCCAGCAC GAGTGGTATT TCAGATCTGG 
GACAATGACA AGTTCTCCTT TGACGACTTT CTGGGCTCCT TGCAGTTGGA TCTCAACCAC 
ATGCCCAAGC CTGCCAAGAC AGCTGAGAAG TGCTCCTTGG ACCAGCTGGA TGACACTTTC 
CACCCGGAGT GGTTTGTGTC CCTTTTTGAA CAGAAAACAG TTAAGGGCTG GTGGCCCTGC 
GTAGCAGAAG AGGGCGAGAA GAAGATCCTG GCAGGCAAGC TGGAAATGAC CTTGGAGATC 
GTAGCAGAGA GTGAGCATGA GGAGCGGCCT GCTGGCCAGG GCCGGGATGA ACCCAACATG 
AACCCCAAGC TCGAGGACCC AAGGCGCCCT GACACCTCCT TCCTCTGGTT CACCTCTCCA 
TACAAGACTA TGAAGTTCAT CCTGTGGCGG CGGTTCCGAT GTGCCATTAT CCTCTTCATC 
ATTCTCTTCA TCCTGCTACT ATTCTTAGGC ATCTTCATCT ATGCTTTCCC GAACTACGCT 
GCCATGAAGC TGGTGAAGCC CTTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DYSF
Entrez Gene ID	483121
Full Name	dysferlin
General protein information	Preferred Names dysferlin Names dysferlin dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Homo sapiens (human)	DYSF	NP_001124454.1
	DYSF	XP_002799336.1
Caenorhabditis elegans (roundworm)	fer-1	NP_492337.1
Caenorhabditis elegans (roundworm)	ferl-1	NP_491602.2
Rattus norvegicus (Norway rat)	Dysf	NP_001101339.1
Danio rerio (zebrafish)	dysf	NP_001244122.1
Mus musculus (house mouse)	Dysf	NP_067444.2
Canis lupus familiaris (dog)	DYSF	XP_003432278.1
Bos taurus (cattle)	DYSF	NP_001095960.1
Pan troglodytes (chimpanzee)	DYSF	XP_003309136.1
Gallus gallus (chicken)	DYSF	XP_423126.4

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DYSF cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

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