ARHGEF5 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARHGEF5
Entrez Gene ID	482718
Full Name	Rho guanine nucleotide exchange factor 5
General protein information	Preferred Names Rho guanine nucleotide exchange factor 5 Names rho guanine nucleotide exchange factor 5
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005629525.2	XP_005629582.1	rho guanine nucleotide exchange factor 5 isoform X2
XM_005629523.1	XP_005629580.1	rho guanine nucleotide exchange factor 5 isoform X1
XM_539834.5	XP_539834.3	rho guanine nucleotide exchange factor 5 isoform X1
XM_005629523.2	XP_005629580.1	rho guanine nucleotide exchange factor 5 isoform X1
XM_005629524.1	XP_005629581.1	rho guanine nucleotide exchange factor 5 isoform X1
XM_014119973.1	XP_013975448.1	rho guanine nucleotide exchange factor 5 isoform X2
XM_005629525.1	XP_005629582.1	rho guanine nucleotide exchange factor 5 isoform X3
XM_539834.4	XP_539834.3	rho guanine nucleotide exchange factor 5 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ARHGEF5	NP_005426.2
	ARHGEF5	XP_001094223.2
Canis lupus familiaris (dog)	ARHGEF5	XP_005629580.1
Pan troglodytes (chimpanzee)	ARHGEF5	XP_519460.3
Mus musculus (house mouse)	Arhgef5	NP_598435.1
Rattus norvegicus (Norway rat)	Arhgef5	XP_342677.4
Bos taurus (cattle)	ARHGEF5	NP_001103545.2

Gene Ontology

The following ARHGEF5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe40509	XM_014119973.1 Latest version!	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCe17929	XM_005629525.2 Latest version!	Canis lupus familiaris Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe17928	XM_005629524.1 Latest version!	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe17928	XM_005629523.2 Latest version!	Canis lupus familiaris Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe17928	XM_539834.5 Latest version!	Canis lupus familiaris Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
OCe17928	XM_005629523.1	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OCe17929	XM_005629525.1	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OCe17928	XM_539834.4	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARHGEF5 ( XM_014119973.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013975448.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe40509
Clone ID Related Accession (Same CDS sequence)	XM_014119973.1
Accession Version	XM_014119973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 5 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GACACCTGTT ATCCCATGAC CGGTACTCCT 
GGGCCTCGGG AGGAGCCTAG GGCTGAGGAA CCATCCCCTG TAGCTCTGCC TCCTCCACTG 
GAGTCCGGAG GGTGGTCGGG CCGGCCTGCT TCTTTACCTG GTTCCTTTCC TGCTGGGGGG 
TCGCCCGCCC AAGAAGCAGC TCAGGACAGC CAGCAGGAGG GACCCGAGCT GGGGAAGAGG 
GCAGTGTTCG GCCAGGGGAC CCAGATGGTC TCTGCCGGTA CCCCTGCAAC TCCTCCGAGG 
ACTCCCCATC CACCTCCTTC CGGTCCTGCT GAAGTCTCCT CCCGGACGAC TACATCATTG 
TCTGCCAGCC CCCCAGTTGG CTCTCCCAGG AGGGAGCCCT TTCTTGCTGC GCATTCTCCA 
GAAACCTCCA GAGCAACTCT TCGGACCACC CACGCACATC ACTGTGGGGC TTCCGAGACT 
CCCCCAGCTG CCCTCCCTGG CGCTTCCTCT GCAGAGGCCA CCGTGGAAAC ACCCACCAGC 
TCCATCTGGG CCCACCCCCA GGGCCCCCCA GGCCACCCTG CAGGGGCTGT CCAGCACCTA 
AGGAGCAACT CCTTCCCAGG CTCTCATAGG ACAGAGCCAA CTCCGGACCT GCTGGGACTC 
TCACTTTCCT TTTCTCATTC AGAGTTGCCC CAAAGGGCCC CCAAACCTGC CATCTATGGC 
TCTGTGATCC CAAGAAGGGA CAGGAAAAGT GGTAGGGACT GTAGGATCAT TCCCGAATCC 
CCTAGTTCAT TATCTACTCT GGGGTGGGAC TCTCAAGAAT TCACTTCAAA TCTGGAAAGA 
TCCAGTAGTC CCTGTAGCAC CCAGCCATGT GGCTCCCCAC ACACTTCAGC CTCTGCCCCA 
GAGTCTCCTG CCTATGGCTC TTCCCCACCC TTTATCTCTG TAGATGGGAG GATCCCTGAA 
CCTCTGCCCC CTCCTCCCCC AGAGAAGAGG CATGTCCACC CCTCCGTGGT AGAGAGAGAC 
AGCCACCTCC ATGCAGGAGT TGCCACGTTG AAGAGGTGTG GCCATGCTCT TCCATTGGCC 
CCAGGTTCGG GGCTACATGG TCCCCCCAAA GGCCCACTTC CCCCATTTCC TGACCCCCTC 
GTGGCAAGGC AGCATCGACC TCTGCCCTCT ACCCCGGACA CTCCCACCCC TACTCTGACC 
TCTTTCTCCC GTAGGCTGAG ATACAATAAG CCATTGCCCC CAACTCCTGA TTTGTCCCAG 
CCCCACCATT CTGTTTCTTT TAGTAATCCA AGGATCTACA GGCCTCTCCC CCCTGTCCCT 
ATTATGGATC CTCCCATTGA ACCACCTCCG TTACCCCCCA AGTCCAAGGG GAGGAGTAGG 
AGCACTCAGG GAGAACTCAT GAATTCAGGG GGTCGGGGTA AGCCAAGGCC TGTTTGTCAA 
GAGCGGACAG TCTCTGCTCC CCATTCTGTT GGACGTACCT CCTGGCCCCC AGCCATGGGC 
CGATCAACAG ACTCTTTGGC TTCTACCAGC AGGAGTAAGA GTGAAGTGTC CCCTGGCATG 
GCTTTCAGCA ATGTGGCAAC CCTTCTCAGT CCGTCTTCCC CAACCACACA CTGGAGTCTG 
GAGCTCCAGG GACCCACCTG TGAACCAGGA CCCTCCGAAG AGTCTGAGGC CCCTGCCAGA 
GGATCTTTGA GAAGATCAGC CCCTCCGGAA GGAGCCAATG GCCTGAGGAG GTTGGATGTA 
GGCCAAGCAA GGCAGTCGGA AAAGCTCAGC CATCCCCACC TGGAGAAGGC ATCCAGCTGG 
CCCCACAGGC GGGACACAGG GAGACCGCTG GAGAGCGTCA GTGGGCGGGT CGCAGATCCC 
GGTGAGGGGT CCAGTAAGCA CAAGGGCTGG AACCGGCAAG GCCTGCGCAG ACCTTCCATC 
TTGCCTGAGG GCTCTTCCGA TACAAGAGGT CCAGGCGTAG AAAAGGCCCC TGGCCCTTCA 
GACACCATTG TGTTTCGGGA GAAGAAACCA AAGGAGGTGA TGGGAGGCTT TTCAAGACGC 
CGCTCGAAGC TCATCAACTC CTCTCAGCTG CTTTACCAGG AATACAGCGA TGTGGTTTTG 
AACAAGGAGA TTCAGAGCCA GCAGCGGCTG GACAGCCTGG GAGAGGCCAC GGGGCCCGCC 
TCCCCCCGGC AGCCCCGGAA GGCTCTGGTC TCCTCCGAAT CCTACCTGCA GCGCCTGTCC 
ATGGCCTCCA GTGGCTCCCT CTGGCAGGAA ATCCCTGTGG TGCGCAACAG CACCGTGCTG 
CTCTCCATGA CCCATGAAGA TCAAAAACTG CAAGAGGCCA AATTTGAGCT GATCGTGTCA 
GAGGCCTCAT ACCTACGCAG TCTGCATGTA GCTGTGGATC ATTTCCAGCT CTCAGCCCCA 
CTGCGGGCCA CCCTTTCCAA CCAGGAATAC CAGTGGCTCT TCTCTCGTTT ACAGGACGTG 
CGTGAAGTCA GCACCACGTT CCTTTCCGAC CTGGAGGAGA ACTTCGAGAG CAACATCTTC 
ACCTTCCAAG TGTGTGACGT AGTCCTGAAC CACGCTCCCG ACTTTCGCCG GGTCTACCTG 
CCGTATGTCA CCAACCAGAC CTACCAGGAG CGCACCTTCC AAGTCCTGCT GAACAGCAAC 
AGCAGTTTCC GAGAGGTCCT GGAGAAGCTG GAGAGCGACC CCGTCTGCCA GCGCCTCTCC 
CTGAAGTCCT TTCTGATCCT GCCCTTCCAG CGCATCACGC GTCTCAAACT GCTGCTCCAG 
AACATTCTGA AGAGAACACA GCCTGGATCT CCAGAGGAAG CAGAGGCCAC AAAGGCACAT 
CATGCGCTGG AGGAGCTGAT CCGAGACTGC AATAACAATG TCCAGAGGAT GCGACGGACA 
GAGGAGCTCA TCTACCTGAG CCAGAAGATT GAGTTCGAGT GTAAAATATT CCCGCTCATT 
TCGCAGTCGC GCTGGCTGGT GAAGAGTGGA GAGGTGACAG CCCTGGAGTT CAGTCTGTCC 
CCAGGGCTGC GAAGGAAGCT CAACACGCGT CCTGTCCACC TGCATCTCTT CAATGACTGT 
CTGTTGCTGT CTCGGCCCCG AGAGGGTAGC CGATTCCTGG TATTTGACCA TGCTCCCTTC 
TCCTCCATCC GAGGGGAAAA GTGTGAAATG AAGCTACACG GACCTCACAA AAACCTCTTC 
CGACTCTTCC TGCTGCACAA CGCGCAGGGC ACCCAGGCCG AGTTCCTCTT CAGCACAGAG 
ACCCAAAGTG AAAAGCTTCG ATGGATCTCA GCCTTGGCCA TGCCCAGAGA GGAGTTGGAC 
CTTCTGGAGT GTTATGACTC CCCTCAAGTA CAGTGCCTTC GAGCCTACAA ACCCCGAGAG 
AACGACGAGC TGGCACTAGA GAAGGCAGAC GTGGTGATGG TGACCCAGCA AAGCAGCGAT 
GGCTGGCTGG AGGGCATGAG ACTCTCAGAT GGGGAGCGAG GCTGGTTCCC TATGCAACAA 
GTGGAGTTCA TTTCCAACCG AGAGGTCCGT GCCCGGAACG TGAGTGAAGC CCACCGAGTC 
AAGACTGCCA AACTACAGCT GGTGGAGCAG CAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013975448.1
CDS	152..4090
Translation	MEAEEPQHGALTPIPALAELSIIPEPLRRSSQTATLGPEAQEGWEPSSTWAEGRGLLEPQQGGLKDVKSCATESMTSFPEEAPAGVETDQEDTVGDTWDTPERQEPVPQSLADGRARTPAHAERGACPFQDTCYPMTGTPGPREEPRAEEPSPVALPPPLESGGWSGRPASLPGSFPAGGSPAQEAAQDSQQEGPELGKRAVFGQGTQMVSAGTPATPPRTPHPPPSGPAEVSSRTTTSLSASPPVGSPRREPFLAAHSPETSRATLRTTHAHHCGASETPPAALPGASSAEATVETPTSSIWAHPQGPPGHPAGAVQHLRSNSFPGSHRTEPTPDLLGLSLSFSHSELPQRAPKPAIYGSVIPRRDRKSGRDCRIIPESPSSLSTLGWDSQEFTSNLERSSSPCSTQPCGSPHTSASAPESPAYGSSPPFISVDGRIPEPLPPPPPEKRHVHPSVVERDSHLHAGVATLKRCGHALPLAPGSGLHGPPKGPLPPFPDPLVARQHRPLPSTPDTPTPTLTSFSRRLRYNKPLPPTPDLSQPHHSVSFSNPRIYRPLPPVPIMDPPIEPPPLPPKSKGRSRSTQGELMNSGGRGKPRPVCQERTVSAPHSVGRTSWPPAMGRSTDSLASTSRSKSEVSPGMAFSNVATLLSPSSPTTHWSLELQGPTCEPGPSEESEAPARGSLRRSAPPEGANGLRRLDVGQARQSEKLSHPHLEKASSWPHRRDTGRPLESVSGRVADPGEGSSKHKGWNRQGLRRPSILPEGSSDTRGPGVEKAPGPSDTIVFREKKPKEVMGGFSRRRSKLINSSQLLYQEYSDVVLNKEIQSQQRLDSLGEATGPASPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLHVAVDHFQLSAPLRATLSNQEYQWLFSRLQDVREVSTTFLSDLEENFESNIFTFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQVLLNSNSSFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSPEEAEATKAHHALEELIRDCNNNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGEVTALEFSLSPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQAEFLFSTETQSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGMRLSDGERGWFPMQQVEFISNREVRARNVSEAHRVKTAKLQLVEQQT

Target ORF information:

RefSeq Version	XM_014119973.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014119973.1

ORF Insert Sequence:

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GACACCTGTT ATCCCATGAC CGGTACTCCT 
GGGCCTCGGG AGGAGCCTAG GGCTGAGGAA CCATCCCCTG TAGCTCTGCC TCCTCCACTG 
GAGTCCGGAG GGTGGTCGGG CCGGCCTGCT TCTTTACCTG GTTCCTTTCC TGCTGGGGGG 
TCGCCCGCCC AAGAAGCAGC TCAGGACAGC CAGCAGGAGG GACCCGAGCT GGGGAAGAGG 
GCAGTGTTCG GCCAGGGGAC CCAGATGGTC TCTGCCGGTA CCCCTGCAAC TCCTCCGAGG 
ACTCCCCATC CACCTCCTTC CGGTCCTGCT GAAGTCTCCT CCCGGACGAC TACATCATTG 
TCTGCCAGCC CCCCAGTTGG CTCTCCCAGG AGGGAGCCCT TTCTTGCTGC GCATTCTCCA 
GAAACCTCCA GAGCAACTCT TCGGACCACC CACGCACATC ACTGTGGGGC TTCCGAGACT 
CCCCCAGCTG CCCTCCCTGG CGCTTCCTCT GCAGAGGCCA CCGTGGAAAC ACCCACCAGC 
TCCATCTGGG CCCACCCCCA GGGCCCCCCA GGCCACCCTG CAGGGGCTGT CCAGCACCTA 
AGGAGCAACT CCTTCCCAGG CTCTCATAGG ACAGAGCCAA CTCCGGACCT GCTGGGACTC 
TCACTTTCCT TTTCTCATTC AGAGTTGCCC CAAAGGGCCC CCAAACCTGC CATCTATGGC 
TCTGTGATCC CAAGAAGGGA CAGGAAAAGT GGTAGGGACT GTAGGATCAT TCCCGAATCC 
CCTAGTTCAT TATCTACTCT GGGGTGGGAC TCTCAAGAAT TCACTTCAAA TCTGGAAAGA 
TCCAGTAGTC CCTGTAGCAC CCAGCCATGT GGCTCCCCAC ACACTTCAGC CTCTGCCCCA 
GAGTCTCCTG CCTATGGCTC TTCCCCACCC TTTATCTCTG TAGATGGGAG GATCCCTGAA 
CCTCTGCCCC CTCCTCCCCC AGAGAAGAGG CATGTCCACC CCTCCGTGGT AGAGAGAGAC 
AGCCACCTCC ATGCAGGAGT TGCCACGTTG AAGAGGTGTG GCCATGCTCT TCCATTGGCC 
CCAGGTTCGG GGCTACATGG TCCCCCCAAA GGCCCACTTC CCCCATTTCC TGACCCCCTC 
GTGGCAAGGC AGCATCGACC TCTGCCCTCT ACCCCGGACA CTCCCACCCC TACTCTGACC 
TCTTTCTCCC GTAGGCTGAG ATACAATAAG CCATTGCCCC CAACTCCTGA TTTGTCCCAG 
CCCCACCATT CTGTTTCTTT TAGTAATCCA AGGATCTACA GGCCTCTCCC CCCTGTCCCT 
ATTATGGATC CTCCCATTGA ACCACCTCCG TTACCCCCCA AGTCCAAGGG GAGGAGTAGG 
AGCACTCAGG GAGAACTCAT GAATTCAGGG GGTCGGGGTA AGCCAAGGCC TGTTTGTCAA 
GAGCGGACAG TCTCTGCTCC CCATTCTGTT GGACGTACCT CCTGGCCCCC AGCCATGGGC 
CGATCAACAG ACTCTTTGGC TTCTACCAGC AGGAGTAAGA GTGAAGTGTC CCCTGGCATG 
GCTTTCAGCA ATGTGGCAAC CCTTCTCAGT CCGTCTTCCC CAACCACACA CTGGAGTCTG 
GAGCTCCAGG GACCCACCTG TGAACCAGGA CCCTCCGAAG AGTCTGAGGC CCCTGCCAGA 
GGATCTTTGA GAAGATCAGC CCCTCCGGAA GGAGCCAATG GCCTGAGGAG GTTGGATGTA 
GGCCAAGCAA GGCAGTCGGA AAAGCTCAGC CATCCCCACC TGGAGAAGGC ATCCAGCTGG 
CCCCACAGGC GGGACACAGG GAGACCGCTG GAGAGCGTCA GTGGGCGGGT CGCAGATCCC 
GGTGAGGGGT CCAGTAAGCA CAAGGGCTGG AACCGGCAAG GCCTGCGCAG ACCTTCCATC 
TTGCCTGAGG GCTCTTCCGA TACAAGAGGT CCAGGCGTAG AAAAGGCCCC TGGCCCTTCA 
GACACCATTG TGTTTCGGGA GAAGAAACCA AAGGAGGTGA TGGGAGGCTT TTCAAGACGC 
CGCTCGAAGC TCATCAACTC CTCTCAGCTG CTTTACCAGG AATACAGCGA TGTGGTTTTG 
AACAAGGAGA TTCAGAGCCA GCAGCGGCTG GACAGCCTGG GAGAGGCCAC GGGGCCCGCC 
TCCCCCCGGC AGCCCCGGAA GGCTCTGGTC TCCTCCGAAT CCTACCTGCA GCGCCTGTCC 
ATGGCCTCCA GTGGCTCCCT CTGGCAGGAA ATCCCTGTGG TGCGCAACAG CACCGTGCTG 
CTCTCCATGA CCCATGAAGA TCAAAAACTG CAAGAGGCCA AATTTGAGCT GATCGTGTCA 
GAGGCCTCAT ACCTACGCAG TCTGCATGTA GCTGTGGATC ATTTCCAGCT CTCAGCCCCA 
CTGCGGGCCA CCCTTTCCAA CCAGGAATAC CAGTGGCTCT TCTCTCGTTT ACAGGACGTG 
CGTGAAGTCA GCACCACGTT CCTTTCCGAC CTGGAGGAGA ACTTCGAGAG CAACATCTTC 
ACCTTCCAAG TGTGTGACGT AGTCCTGAAC CACGCTCCCG ACTTTCGCCG GGTCTACCTG 
CCGTATGTCA CCAACCAGAC CTACCAGGAG CGCACCTTCC AAGTCCTGCT GAACAGCAAC 
AGCAGTTTCC GAGAGGTCCT GGAGAAGCTG GAGAGCGACC CCGTCTGCCA GCGCCTCTCC 
CTGAAGTCCT TTCTGATCCT GCCCTTCCAG CGCATCACGC GTCTCAAACT GCTGCTCCAG 
AACATTCTGA AGAGAACACA GCCTGGATCT CCAGAGGAAG CAGAGGCCAC AAAGGCACAT 
CATGCGCTGG AGGAGCTGAT CCGAGACTGC AATAACAATG TCCAGAGGAT GCGACGGACA 
GAGGAGCTCA TCTACCTGAG CCAGAAGATT GAGTTCGAGT GTAAAATATT CCCGCTCATT 
TCGCAGTCGC GCTGGCTGGT GAAGAGTGGA GAGGTGACAG CCCTGGAGTT CAGTCTGTCC 
CCAGGGCTGC GAAGGAAGCT CAACACGCGT CCTGTCCACC TGCATCTCTT CAATGACTGT 
CTGTTGCTGT CTCGGCCCCG AGAGGGTAGC CGATTCCTGG TATTTGACCA TGCTCCCTTC 
TCCTCCATCC GAGGGGAAAA GTGTGAAATG AAGCTACACG GACCTCACAA AAACCTCTTC 
CGACTCTTCC TGCTGCACAA CGCGCAGGGC ACCCAGGCCG AGTTCCTCTT CAGCACAGAG 
ACCCAAAGTG AAAAGCTTCG ATGGATCTCA GCCTTGGCCA TGCCCAGAGA GGAGTTGGAC 
CTTCTGGAGT GTTATGACTC CCCTCAAGTA CAGTGCCTTC GAGCCTACAA ACCCCGAGAG 
AACGACGAGC TGGCACTAGA GAAGGCAGAC GTGGTGATGG TGACCCAGCA AAGCAGCGAT 
GGCTGGCTGG AGGGCATGAG ACTCTCAGAT GGGGAGCGAG GCTGGTTCCC TATGCAACAA 
GTGGAGTTCA TTTCCAACCG AGAGGTCCGT GCCCGGAACG TGAGTGAAGC CCACCGAGTC 
AAGACTGCCA AACTACAGCT GGTGGAGCAG CAGACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( XM_005629525.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005629582.1 Canis lupus familiaris Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe17929
Clone ID Related Accession (Same CDS sequence)	XM_005629525.2 , XM_005629525.1
Accession Version	XM_005629525.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629525.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGACACCTG TTATCCCATG ACCGGTACTC CTGGGCCTCG GGAGGAGCCT 
AGGGCTGAGG AACCATCCCC TGTAGCTCTG CCTCCTCCAC TGGAGTCCGG AGGGTGGTCG 
GGCCGGCCTG CTTCTTTACC TGGTTCCTTT CCTGCTGGGG GGTCGCCCGC CCAAGAAGCA 
GCTCAGGACA GCCAGCAGGA GGGACCCGAG CTGGGGAAGA GGGCAGTGTT CGGCCAGGGG 
ACCCAGATGG TCTCTGCCGG TACCCCTGCA ACTCCTCCGA GGACTCCCCA TCCACCTCCT 
TCCGGTCCTG CTGAAGTCTC CTCCCGGACG ACTACATCAT TGTCTGCCAG CCCCCCAGTT 
GGCTCTCCCA GGAGGGAGCC CTTTCTTGCT GCGCATTCTC CAGAAACCTC CAGAGCAACT 
CTTCGGACCA CCCACGCACA TCACTGTGGG GCTTCCGAGA CTCCCCCAGC TGCCCTCCCT 
GGCGCTTCCT CTGCAGAGGC CACCGTGGAA ACACCCACCA GCTCCATCTG GGCCCACCCC 
CAGGGCCCCC CAGGCCACCC TGCAGGGGCT GTCCAGCACC TAAGGAGCAA CTCCTTCCCA 
GGCTCTCATA GGACAGAGCC AACTCCGGAC CTGCTGGGAC TCTCACTTTC CTTTTCTCAT 
TCAGAGTTGC CCCAAAGGGC CCCCAAACCT GCCATCTATG GCTCTGTGAT CCCAAGAAGG 
GACAGGAAAA GTGGTAGGGA CTGTAGGATC ATTCCCGAAT CCCCTAGTTC ATTATCTACT 
CTGGGGTGGG ACTCTCAAGA ATTCACTTCA AATCTGGAAA GATCCAGTAG TCCCTGTAGC 
ACCCAGCCAT GTGGCTCCCC ACACACTTCA GCCTCTGCCC CAGAGTCTCC TGCCTATGGC 
TCTTCCCCAC CCTTTATCTC TGTAGATGGG AGGATCCCTG AACCTCTGCC CCCTCCTCCC 
CCAGAGAAGA GGCATGTCCA CCCCTCCGTG GTAGAGAGAG ACAGCCACCT CCATGCAGGA 
GTTGCCACGT TGAAGAGGTG TGGCCATGCT CTTCCATTGG CCCCAGGTTC GGGGCTACAT 
GGTCCCCCCA AAGGCCCACT TCCCCCATTT CCTGACCCCC TCGTGGCAAG GCAGCATCGA 
CCTCTGCCCT CTACCCCGGA CACTCCCACC CCTACTCTGA CCTCTTTCTC CCGTAGGCTG 
AGATACAATA AGCCATTGCC CCCAACTCCT GATTTGTCCC AGCCCCACCA TTCTGTTTCT 
TTTAGTAATC CAAGGATCTA CAGGCCTCTC CCCCCTGTCC CTATTATGGA TCCTCCCATT 
GAACCACCTC CGTTACCCCC CAAGTCCAAG GGGAGGAGTA GGAGCACTCA GGGAGAACTC 
ATGAATTCAG GGGGTCGGGG TAAGCCAAGG CCTGTTTGTC AAGAGCGGAC AGTCTCTGCT 
CCCCATTCTG TTGGACGTAC CTCCTGGCCC CCAGCCATGG GCCGATCAAC AGACTCTTTG 
GCTTCTACCA GCAGGAGTAA GAGTGAAGTG TCCCCTGGCA TGGCTTTCAG CAATGTGGCA 
ACCCTTCTCA GTCCGTCTTC CCCAACCACA CACTGGAGTC TGGAGCTCCA GGGACCCACC 
TGTGAACCAG GACCCTCCGA AGAGTCTGAG GCCCCTGCCA GAGGATCTTT GAGAAGATCA 
GCCCCTCCGG AAGGAGCCAA TGGCCTGAGG AGGTTGGATG TAGGCCAAGC AAGGCAGTCG 
GAAAAGCTCA GCCATCCCCA CCTGGAGAAG GCATCCAGCT GGCCCCACAG GCGGGACACA 
GGGAGACCGC TGGAGAGCGT CAGTGGGCGG GTCGCAGATC CCGGTGAGGG GTCCAGTAAG 
CACAAGGGCT GGAACCGGCA AGGCCTGCGC AGACCTTCCA TCTTGCCTGA GGGCTCTTCC 
GATACAAGAG GTCCAGGCGT AGAAAAGGCC CCTGGCCCTT CAGACACCAT TGTGTTTCGG 
GAGAAGAAAC CAAAGGAGGT GATGGGAGGC TTTTCAAGAC GCCGCTCGAA GCTCATCAAC 
TCCTCTCAGC TGCTTTACCA GGAATACAGC GATGTGGTTT TGAACAAGGA GATTCAGAGC 
CAGCAGCGGC TGGACAGCCT GGGAGAGGCC ACGGGGCCCG CCTCCCCCCG GCAGCCCCGG 
AAGGCTCTGG TCTCCTCCGA ATCCTACCTG CAGCGCCTGT CCATGGCCTC CAGTGGCTCC 
CTCTGGCAGG AAATCCCTGT GGTGCGCAAC AGCACCGTGC TGCTCTCCAT GACCCATGAA 
GATCAAAAAC TGCAAGAGGC CAAATTTGAG CTGATCGTGT CAGAGGCCTC ATACCTACGC 
AGTCTGCATG TAGCTGTGGA TCATTTCCAG CTCTCAGCCC CACTGCGGGC CACCCTTTCC 
AACCAGGAAT ACCAGTGGCT CTTCTCTCGT TTACAGGACG TGCGTGAAGT CAGCACCACG 
TTCCTTTCCG ACCTGGAGGA GAACTTCGAG AGCAACATCT TCACCTTCCA AGTGTGTGAC 
GTAGTCCTGA ACCACGCTCC CGACTTTCGC CGGGTCTACC TGCCGTATGT CACCAACCAG 
ACCTACCAGG AGCGCACCTT CCAAGTCCTG CTGAACAGCA ACAGCAGTTT CCGAGAGGTC 
CTGGAGAAGC TGGAGAGCGA CCCCGTCTGC CAGCGCCTCT CCCTGAAGTC CTTTCTGATC 
CTGCCCTTCC AGCGCATCAC GCGTCTCAAA CTGCTGCTCC AGAACATTCT GAAGAGAACA 
CAGCCTGGAT CTCCAGAGGA AGCAGAGGCC ACAAAGGCAC ATCATGCGCT GGAGGAGCTG 
ATCCGAGACT GCAATAACAA TGTCCAGAGG ATGCGACGGA CAGAGGAGCT CATCTACCTG 
AGCCAGAAGA TTGAGTTCGA GTGTAAAATA TTCCCGCTCA TTTCGCAGTC GCGCTGGCTG 
GTGAAGAGTG GAGAGGTGAC AGCCCTGGAG TTCAGTCTGT CCCCAGGGCT GCGAAGGAAG 
CTCAACACGC GTCCTGTCCA CCTGCATCTC TTCAATGACT GTCTGTTGCT GTCTCGGCCC 
CGAGAGGGTA GCCGATTCCT GGTATTTGAC CATGCTCCCT TCTCCTCCAT CCGAGGGGAA 
AAGTGTGAAA TGAAGCTACA CGGACCTCAC AAAAACCTCT TCCGACTCTT CCTGCTGCAC 
AACGCGCAGG GCACCCAGGC CGAGTTCCTC TTCAGCACAG AGACCCAAAG TGAAAAGCTT 
CGATGGATCT CAGCCTTGGC CATGCCCAGA GAGGAGTTGG ACCTTCTGGA GTGTTATGAC 
TCCCCTCAAG TACAGTGCCT TCGAGCCTAC AAACCCCGAG AGAACGACGA GCTGGCACTA 
GAGAAGGCAG ACGTGGTGAT GGTGACCCAG CAAAGCAGCG ATGGCTGGCT GGAGGGCATG 
AGACTCTCAG ATGGGGAGCG AGGCTGGTTC CCTATGCAAC AAGTGGAGTT CATTTCCAAC 
CGAGAGGTCC GTGCCCGGAA CGTGAGTGAA GCCCACCGAG TCAAGACTGC CAAACTACAG 
CTGGTGGAGC AGCAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629582.1
CDS	117..3917
Translation	MEAEEPQHGALTPIPALAELSIIPEPLRRSSQTATLGPEAQEGWEPSSTWAEGRGLLEPQQGGLKDVKSCATESMTSFPEEAPADTCYPMTGTPGPREEPRAEEPSPVALPPPLESGGWSGRPASLPGSFPAGGSPAQEAAQDSQQEGPELGKRAVFGQGTQMVSAGTPATPPRTPHPPPSGPAEVSSRTTTSLSASPPVGSPRREPFLAAHSPETSRATLRTTHAHHCGASETPPAALPGASSAEATVETPTSSIWAHPQGPPGHPAGAVQHLRSNSFPGSHRTEPTPDLLGLSLSFSHSELPQRAPKPAIYGSVIPRRDRKSGRDCRIIPESPSSLSTLGWDSQEFTSNLERSSSPCSTQPCGSPHTSASAPESPAYGSSPPFISVDGRIPEPLPPPPPEKRHVHPSVVERDSHLHAGVATLKRCGHALPLAPGSGLHGPPKGPLPPFPDPLVARQHRPLPSTPDTPTPTLTSFSRRLRYNKPLPPTPDLSQPHHSVSFSNPRIYRPLPPVPIMDPPIEPPPLPPKSKGRSRSTQGELMNSGGRGKPRPVCQERTVSAPHSVGRTSWPPAMGRSTDSLASTSRSKSEVSPGMAFSNVATLLSPSSPTTHWSLELQGPTCEPGPSEESEAPARGSLRRSAPPEGANGLRRLDVGQARQSEKLSHPHLEKASSWPHRRDTGRPLESVSGRVADPGEGSSKHKGWNRQGLRRPSILPEGSSDTRGPGVEKAPGPSDTIVFREKKPKEVMGGFSRRRSKLINSSQLLYQEYSDVVLNKEIQSQQRLDSLGEATGPASPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLHVAVDHFQLSAPLRATLSNQEYQWLFSRLQDVREVSTTFLSDLEENFESNIFTFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQVLLNSNSSFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSPEEAEATKAHHALEELIRDCNNNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGEVTALEFSLSPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQAEFLFSTETQSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGMRLSDGERGWFPMQQVEFISNREVRARNVSEAHRVKTAKLQLVEQQT

Target ORF information:

RefSeq Version	XM_005629525.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629525.2

ORF Insert Sequence:

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGACACCTG TTATCCCATG ACCGGTACTC CTGGGCCTCG GGAGGAGCCT 
AGGGCTGAGG AACCATCCCC TGTAGCTCTG CCTCCTCCAC TGGAGTCCGG AGGGTGGTCG 
GGCCGGCCTG CTTCTTTACC TGGTTCCTTT CCTGCTGGGG GGTCGCCCGC CCAAGAAGCA 
GCTCAGGACA GCCAGCAGGA GGGACCCGAG CTGGGGAAGA GGGCAGTGTT CGGCCAGGGG 
ACCCAGATGG TCTCTGCCGG TACCCCTGCA ACTCCTCCGA GGACTCCCCA TCCACCTCCT 
TCCGGTCCTG CTGAAGTCTC CTCCCGGACG ACTACATCAT TGTCTGCCAG CCCCCCAGTT 
GGCTCTCCCA GGAGGGAGCC CTTTCTTGCT GCGCATTCTC CAGAAACCTC CAGAGCAACT 
CTTCGGACCA CCCACGCACA TCACTGTGGG GCTTCCGAGA CTCCCCCAGC TGCCCTCCCT 
GGCGCTTCCT CTGCAGAGGC CACCGTGGAA ACACCCACCA GCTCCATCTG GGCCCACCCC 
CAGGGCCCCC CAGGCCACCC TGCAGGGGCT GTCCAGCACC TAAGGAGCAA CTCCTTCCCA 
GGCTCTCATA GGACAGAGCC AACTCCGGAC CTGCTGGGAC TCTCACTTTC CTTTTCTCAT 
TCAGAGTTGC CCCAAAGGGC CCCCAAACCT GCCATCTATG GCTCTGTGAT CCCAAGAAGG 
GACAGGAAAA GTGGTAGGGA CTGTAGGATC ATTCCCGAAT CCCCTAGTTC ATTATCTACT 
CTGGGGTGGG ACTCTCAAGA ATTCACTTCA AATCTGGAAA GATCCAGTAG TCCCTGTAGC 
ACCCAGCCAT GTGGCTCCCC ACACACTTCA GCCTCTGCCC CAGAGTCTCC TGCCTATGGC 
TCTTCCCCAC CCTTTATCTC TGTAGATGGG AGGATCCCTG AACCTCTGCC CCCTCCTCCC 
CCAGAGAAGA GGCATGTCCA CCCCTCCGTG GTAGAGAGAG ACAGCCACCT CCATGCAGGA 
GTTGCCACGT TGAAGAGGTG TGGCCATGCT CTTCCATTGG CCCCAGGTTC GGGGCTACAT 
GGTCCCCCCA AAGGCCCACT TCCCCCATTT CCTGACCCCC TCGTGGCAAG GCAGCATCGA 
CCTCTGCCCT CTACCCCGGA CACTCCCACC CCTACTCTGA CCTCTTTCTC CCGTAGGCTG 
AGATACAATA AGCCATTGCC CCCAACTCCT GATTTGTCCC AGCCCCACCA TTCTGTTTCT 
TTTAGTAATC CAAGGATCTA CAGGCCTCTC CCCCCTGTCC CTATTATGGA TCCTCCCATT 
GAACCACCTC CGTTACCCCC CAAGTCCAAG GGGAGGAGTA GGAGCACTCA GGGAGAACTC 
ATGAATTCAG GGGGTCGGGG TAAGCCAAGG CCTGTTTGTC AAGAGCGGAC AGTCTCTGCT 
CCCCATTCTG TTGGACGTAC CTCCTGGCCC CCAGCCATGG GCCGATCAAC AGACTCTTTG 
GCTTCTACCA GCAGGAGTAA GAGTGAAGTG TCCCCTGGCA TGGCTTTCAG CAATGTGGCA 
ACCCTTCTCA GTCCGTCTTC CCCAACCACA CACTGGAGTC TGGAGCTCCA GGGACCCACC 
TGTGAACCAG GACCCTCCGA AGAGTCTGAG GCCCCTGCCA GAGGATCTTT GAGAAGATCA 
GCCCCTCCGG AAGGAGCCAA TGGCCTGAGG AGGTTGGATG TAGGCCAAGC AAGGCAGTCG 
GAAAAGCTCA GCCATCCCCA CCTGGAGAAG GCATCCAGCT GGCCCCACAG GCGGGACACA 
GGGAGACCGC TGGAGAGCGT CAGTGGGCGG GTCGCAGATC CCGGTGAGGG GTCCAGTAAG 
CACAAGGGCT GGAACCGGCA AGGCCTGCGC AGACCTTCCA TCTTGCCTGA GGGCTCTTCC 
GATACAAGAG GTCCAGGCGT AGAAAAGGCC CCTGGCCCTT CAGACACCAT TGTGTTTCGG 
GAGAAGAAAC CAAAGGAGGT GATGGGAGGC TTTTCAAGAC GCCGCTCGAA GCTCATCAAC 
TCCTCTCAGC TGCTTTACCA GGAATACAGC GATGTGGTTT TGAACAAGGA GATTCAGAGC 
CAGCAGCGGC TGGACAGCCT GGGAGAGGCC ACGGGGCCCG CCTCCCCCCG GCAGCCCCGG 
AAGGCTCTGG TCTCCTCCGA ATCCTACCTG CAGCGCCTGT CCATGGCCTC CAGTGGCTCC 
CTCTGGCAGG AAATCCCTGT GGTGCGCAAC AGCACCGTGC TGCTCTCCAT GACCCATGAA 
GATCAAAAAC TGCAAGAGGC CAAATTTGAG CTGATCGTGT CAGAGGCCTC ATACCTACGC 
AGTCTGCATG TAGCTGTGGA TCATTTCCAG CTCTCAGCCC CACTGCGGGC CACCCTTTCC 
AACCAGGAAT ACCAGTGGCT CTTCTCTCGT TTACAGGACG TGCGTGAAGT CAGCACCACG 
TTCCTTTCCG ACCTGGAGGA GAACTTCGAG AGCAACATCT TCACCTTCCA AGTGTGTGAC 
GTAGTCCTGA ACCACGCTCC CGACTTTCGC CGGGTCTACC TGCCGTATGT CACCAACCAG 
ACCTACCAGG AGCGCACCTT CCAAGTCCTG CTGAACAGCA ACAGCAGTTT CCGAGAGGTC 
CTGGAGAAGC TGGAGAGCGA CCCCGTCTGC CAGCGCCTCT CCCTGAAGTC CTTTCTGATC 
CTGCCCTTCC AGCGCATCAC GCGTCTCAAA CTGCTGCTCC AGAACATTCT GAAGAGAACA 
CAGCCTGGAT CTCCAGAGGA AGCAGAGGCC ACAAAGGCAC ATCATGCGCT GGAGGAGCTG 
ATCCGAGACT GCAATAACAA TGTCCAGAGG ATGCGACGGA CAGAGGAGCT CATCTACCTG 
AGCCAGAAGA TTGAGTTCGA GTGTAAAATA TTCCCGCTCA TTTCGCAGTC GCGCTGGCTG 
GTGAAGAGTG GAGAGGTGAC AGCCCTGGAG TTCAGTCTGT CCCCAGGGCT GCGAAGGAAG 
CTCAACACGC GTCCTGTCCA CCTGCATCTC TTCAATGACT GTCTGTTGCT GTCTCGGCCC 
CGAGAGGGTA GCCGATTCCT GGTATTTGAC CATGCTCCCT TCTCCTCCAT CCGAGGGGAA 
AAGTGTGAAA TGAAGCTACA CGGACCTCAC AAAAACCTCT TCCGACTCTT CCTGCTGCAC 
AACGCGCAGG GCACCCAGGC CGAGTTCCTC TTCAGCACAG AGACCCAAAG TGAAAAGCTT 
CGATGGATCT CAGCCTTGGC CATGCCCAGA GAGGAGTTGG ACCTTCTGGA GTGTTATGAC 
TCCCCTCAAG TACAGTGCCT TCGAGCCTAC AAACCCCGAG AGAACGACGA GCTGGCACTA 
GAGAAGGCAG ACGTGGTGAT GGTGACCCAG CAAAGCAGCG ATGGCTGGCT GGAGGGCATG 
AGACTCTCAG ATGGGGAGCG AGGCTGGTTC CCTATGCAAC AAGTGGAGTT CATTTCCAAC 
CGAGAGGTCC GTGCCCGGAA CGTGAGTGAA GCCCACCGAG TCAAGACTGC CAAACTACAG 
CTGGTGGAGC AGCAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( XM_005629525.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005629582.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X5, mRNA.

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CloneID	OCe17929
Clone ID Related Accession (Same CDS sequence)	XM_005629525.2 , XM_005629525.1
Accession Version	XM_005629525.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3801bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 5 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGACACCTG TTATCCCATG ACCGGTACTC CTGGGCCTCG GGAGGAGCCT 
AGGGCTGAGG AACCATCCCC TGTAGCTCTG CCTCCTCCAC TGGAGTCCGG AGGGTGGTCG 
GGCCGGCCTG CTTCTTTACC TGGTTCCTTT CCTGCTGGGG GGTCGCCCGC CCAAGAAGCA 
GCTCAGGACA GCCAGCAGGA GGGACCCGAG CTGGGGAAGA GGGCAGTGTT CGGCCAGGGG 
ACCCAGATGG TCTCTGCCGG TACCCCTGCA ACTCCTCCGA GGACTCCCCA TCCACCTCCT 
TCCGGTCCTG CTGAAGTCTC CTCCCGGACG ACTACATCAT TGTCTGCCAG CCCCCCAGTT 
GGCTCTCCCA GGAGGGAGCC CTTTCTTGCT GCGCATTCTC CAGAAACCTC CAGAGCAACT 
CTTCGGACCA CCCACGCACA TCACTGTGGG GCTTCCGAGA CTCCCCCAGC TGCCCTCCCT 
GGCGCTTCCT CTGCAGAGGC CACCGTGGAA ACACCCACCA GCTCCATCTG GGCCCACCCC 
CAGGGCCCCC CAGGCCACCC TGCAGGGGCT GTCCAGCACC TAAGGAGCAA CTCCTTCCCA 
GGCTCTCATA GGACAGAGCC AACTCCGGAC CTGCTGGGAC TCTCACTTTC CTTTTCTCAT 
TCAGAGTTGC CCCAAAGGGC CCCCAAACCT GCCATCTATG GCTCTGTGAT CCCAAGAAGG 
GACAGGAAAA GTGGTAGGGA CTGTAGGATC ATTCCCGAAT CCCCTAGTTC ATTATCTACT 
CTGGGGTGGG ACTCTCAAGA ATTCACTTCA AATCTGGAAA GATCCAGTAG TCCCTGTAGC 
ACCCAGCCAT GTGGCTCCCC ACACACTTCA GCCTCTGCCC CAGAGTCTCC TGCCTATGGC 
TCTTCCCCAC CCTTTATCTC TGTAGATGGG AGGATCCCTG AACCTCTGCC CCCTCCTCCC 
CCAGAGAAGA GGCATGTCCA CCCCTCCGTG GTAGAGAGAG ACAGCCACCT CCATGCAGGA 
GTTGCCACGT TGAAGAGGTG TGGCCATGCT CTTCCATTGG CCCCAGGTTC GGGGCTACAT 
GGTCCCCCCA AAGGCCCACT TCCCCCATTT CCTGACCCCC TCGTGGCAAG GCAGCATCGA 
CCTCTGCCCT CTACCCCGGA CACTCCCACC CCTACTCTGA CCTCTTTCTC CCGTAGGCTG 
AGATACAATA AGCCATTGCC CCCAACTCCT GATTTGTCCC AGCCCCACCA TTCTGTTTCT 
TTTAGTAATC CAAGGATCTA CAGGCCTCTC CCCCCTGTCC CTATTATGGA TCCTCCCATT 
GAACCACCTC CGTTACCCCC CAAGTCCAAG GGGAGGAGTA GGAGCACTCA GGGAGAACTC 
ATGAATTCAG GGGGTCGGGG TAAGCCAAGG CCTGTTTGTC AAGAGCGGAC AGTCTCTGCT 
CCCCATTCTG TTGGACGTAC CTCCTGGCCC CCAGCCATGG GCCGATCAAC AGACTCTTTG 
GCTTCTACCA GCAGGAGTAA GAGTGAAGTG TCCCCTGGCA TGGCTTTCAG CAATGTGGCA 
ACCCTTCTCA GTCCGTCTTC CCCAACCACA CACTGGAGTC TGGAGCTCCA GGGACCCACC 
TGTGAACCAG GACCCTCCGA AGAGTCTGAG GCCCCTGCCA GAGGATCTTT GAGAAGATCA 
GCCCCTCCGG AAGGAGCCAA TGGCCTGAGG AGGTTGGATG TAGGCCAAGC AAGGCAGTCG 
GAAAAGCTCA GCCATCCCCA CCTGGAGAAG GCATCCAGCT GGCCCCACAG GCGGGACACA 
GGGAGACCGC TGGAGAGCGT CAGTGGGCGG GTCGCAGATC CCGGTGAGGG GTCCAGTAAG 
CACAAGGGCT GGAACCGGCA AGGCCTGCGC AGACCTTCCA TCTTGCCTGA GGGCTCTTCC 
GATACAAGAG GTCCAGGCGT AGAAAAGGCC CCTGGCCCTT CAGACACCAT TGTGTTTCGG 
GAGAAGAAAC CAAAGGAGGT GATGGGAGGC TTTTCAAGAC GCCGCTCGAA GCTCATCAAC 
TCCTCTCAGC TGCTTTACCA GGAATACAGC GATGTGGTTT TGAACAAGGA GATTCAGAGC 
CAGCAGCGGC TGGACAGCCT GGGAGAGGCC ACGGGGCCCG CCTCCCCCCG GCAGCCCCGG 
AAGGCTCTGG TCTCCTCCGA ATCCTACCTG CAGCGCCTGT CCATGGCCTC CAGTGGCTCC 
CTCTGGCAGG AAATCCCTGT GGTGCGCAAC AGCACCGTGC TGCTCTCCAT GACCCATGAA 
GATCAAAAAC TGCAAGAGGC CAAATTTGAG CTGATCGTGT CAGAGGCCTC ATACCTACGC 
AGTCTGCATG TAGCTGTGGA TCATTTCCAG CTCTCAGCCC CACTGCGGGC CACCCTTTCC 
AACCAGGAAT ACCAGTGGCT CTTCTCTCGT TTACAGGACG TGCGTGAAGT CAGCACCACG 
TTCCTTTCCG ACCTGGAGGA GAACTTCGAG AGCAACATCT TCACCTTCCA AGTGTGTGAC 
GTAGTCCTGA ACCACGCTCC CGACTTTCGC CGGGTCTACC TGCCGTATGT CACCAACCAG 
ACCTACCAGG AGCGCACCTT CCAAGTCCTG CTGAACAGCA ACAGCAGTTT CCGAGAGGTC 
CTGGAGAAGC TGGAGAGCGA CCCCGTCTGC CAGCGCCTCT CCCTGAAGTC CTTTCTGATC 
CTGCCCTTCC AGCGCATCAC GCGTCTCAAA CTGCTGCTCC AGAACATTCT GAAGAGAACA 
CAGCCTGGAT CTCCAGAGGA AGCAGAGGCC ACAAAGGCAC ATCATGCGCT GGAGGAGCTG 
ATCCGAGACT GCAATAACAA TGTCCAGAGG ATGCGACGGA CAGAGGAGCT CATCTACCTG 
AGCCAGAAGA TTGAGTTCGA GTGTAAAATA TTCCCGCTCA TTTCGCAGTC GCGCTGGCTG 
GTGAAGAGTG GAGAGGTGAC AGCCCTGGAG TTCAGTCTGT CCCCAGGGCT GCGAAGGAAG 
CTCAACACGC GTCCTGTCCA CCTGCATCTC TTCAATGACT GTCTGTTGCT GTCTCGGCCC 
CGAGAGGGTA GCCGATTCCT GGTATTTGAC CATGCTCCCT TCTCCTCCAT CCGAGGGGAA 
AAGTGTGAAA TGAAGCTACA CGGACCTCAC AAAAACCTCT TCCGACTCTT CCTGCTGCAC 
AACGCGCAGG GCACCCAGGC CGAGTTCCTC TTCAGCACAG AGACCCAAAG TGAAAAGCTT 
CGATGGATCT CAGCCTTGGC CATGCCCAGA GAGGAGTTGG ACCTTCTGGA GTGTTATGAC 
TCCCCTCAAG TACAGTGCCT TCGAGCCTAC AAACCCCGAG AGAACGACGA GCTGGCACTA 
GAGAAGGCAG ACGTGGTGAT GGTGACCCAG CAAAGCAGCG ATGGCTGGCT GGAGGGCATG 
AGACTCTCAG ATGGGGAGCG AGGCTGGTTC CCTATGCAAC AAGTGGAGTT CATTTCCAAC 
CGAGAGGTCC GTGCCCGGAA CGTGAGTGAA GCCCACCGAG TCAAGACTGC CAAACTACAG 
CTGGTGGAGC AGCAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629582.1
CDS	152..3952
Translation	MEAEEPQHGALTPIPALAELSIIPEPLRRSSQTATLGPEAQEGWEPSSTWAEGRGLLEPQQGGLKDVKSCATESMTSFPEEAPADTCYPMTGTPGPREEPRAEEPSPVALPPPLESGGWSGRPASLPGSFPAGGSPAQEAAQDSQQEGPELGKRAVFGQGTQMVSAGTPATPPRTPHPPPSGPAEVSSRTTTSLSASPPVGSPRREPFLAAHSPETSRATLRTTHAHHCGASETPPAALPGASSAEATVETPTSSIWAHPQGPPGHPAGAVQHLRSNSFPGSHRTEPTPDLLGLSLSFSHSELPQRAPKPAIYGSVIPRRDRKSGRDCRIIPESPSSLSTLGWDSQEFTSNLERSSSPCSTQPCGSPHTSASAPESPAYGSSPPFISVDGRIPEPLPPPPPEKRHVHPSVVERDSHLHAGVATLKRCGHALPLAPGSGLHGPPKGPLPPFPDPLVARQHRPLPSTPDTPTPTLTSFSRRLRYNKPLPPTPDLSQPHHSVSFSNPRIYRPLPPVPIMDPPIEPPPLPPKSKGRSRSTQGELMNSGGRGKPRPVCQERTVSAPHSVGRTSWPPAMGRSTDSLASTSRSKSEVSPGMAFSNVATLLSPSSPTTHWSLELQGPTCEPGPSEESEAPARGSLRRSAPPEGANGLRRLDVGQARQSEKLSHPHLEKASSWPHRRDTGRPLESVSGRVADPGEGSSKHKGWNRQGLRRPSILPEGSSDTRGPGVEKAPGPSDTIVFREKKPKEVMGGFSRRRSKLINSSQLLYQEYSDVVLNKEIQSQQRLDSLGEATGPASPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLHVAVDHFQLSAPLRATLSNQEYQWLFSRLQDVREVSTTFLSDLEENFESNIFTFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQVLLNSNSSFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSPEEAEATKAHHALEELIRDCNNNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGEVTALEFSLSPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQAEFLFSTETQSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGMRLSDGERGWFPMQQVEFISNREVRARNVSEAHRVKTAKLQLVEQQT

Target ORF information:

RefSeq Version	XM_005629525.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629525.1

ORF Insert Sequence:

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGACACCTG TTATCCCATG ACCGGTACTC CTGGGCCTCG GGAGGAGCCT 
AGGGCTGAGG AACCATCCCC TGTAGCTCTG CCTCCTCCAC TGGAGTCCGG AGGGTGGTCG 
GGCCGGCCTG CTTCTTTACC TGGTTCCTTT CCTGCTGGGG GGTCGCCCGC CCAAGAAGCA 
GCTCAGGACA GCCAGCAGGA GGGACCCGAG CTGGGGAAGA GGGCAGTGTT CGGCCAGGGG 
ACCCAGATGG TCTCTGCCGG TACCCCTGCA ACTCCTCCGA GGACTCCCCA TCCACCTCCT 
TCCGGTCCTG CTGAAGTCTC CTCCCGGACG ACTACATCAT TGTCTGCCAG CCCCCCAGTT 
GGCTCTCCCA GGAGGGAGCC CTTTCTTGCT GCGCATTCTC CAGAAACCTC CAGAGCAACT 
CTTCGGACCA CCCACGCACA TCACTGTGGG GCTTCCGAGA CTCCCCCAGC TGCCCTCCCT 
GGCGCTTCCT CTGCAGAGGC CACCGTGGAA ACACCCACCA GCTCCATCTG GGCCCACCCC 
CAGGGCCCCC CAGGCCACCC TGCAGGGGCT GTCCAGCACC TAAGGAGCAA CTCCTTCCCA 
GGCTCTCATA GGACAGAGCC AACTCCGGAC CTGCTGGGAC TCTCACTTTC CTTTTCTCAT 
TCAGAGTTGC CCCAAAGGGC CCCCAAACCT GCCATCTATG GCTCTGTGAT CCCAAGAAGG 
GACAGGAAAA GTGGTAGGGA CTGTAGGATC ATTCCCGAAT CCCCTAGTTC ATTATCTACT 
CTGGGGTGGG ACTCTCAAGA ATTCACTTCA AATCTGGAAA GATCCAGTAG TCCCTGTAGC 
ACCCAGCCAT GTGGCTCCCC ACACACTTCA GCCTCTGCCC CAGAGTCTCC TGCCTATGGC 
TCTTCCCCAC CCTTTATCTC TGTAGATGGG AGGATCCCTG AACCTCTGCC CCCTCCTCCC 
CCAGAGAAGA GGCATGTCCA CCCCTCCGTG GTAGAGAGAG ACAGCCACCT CCATGCAGGA 
GTTGCCACGT TGAAGAGGTG TGGCCATGCT CTTCCATTGG CCCCAGGTTC GGGGCTACAT 
GGTCCCCCCA AAGGCCCACT TCCCCCATTT CCTGACCCCC TCGTGGCAAG GCAGCATCGA 
CCTCTGCCCT CTACCCCGGA CACTCCCACC CCTACTCTGA CCTCTTTCTC CCGTAGGCTG 
AGATACAATA AGCCATTGCC CCCAACTCCT GATTTGTCCC AGCCCCACCA TTCTGTTTCT 
TTTAGTAATC CAAGGATCTA CAGGCCTCTC CCCCCTGTCC CTATTATGGA TCCTCCCATT 
GAACCACCTC CGTTACCCCC CAAGTCCAAG GGGAGGAGTA GGAGCACTCA GGGAGAACTC 
ATGAATTCAG GGGGTCGGGG TAAGCCAAGG CCTGTTTGTC AAGAGCGGAC AGTCTCTGCT 
CCCCATTCTG TTGGACGTAC CTCCTGGCCC CCAGCCATGG GCCGATCAAC AGACTCTTTG 
GCTTCTACCA GCAGGAGTAA GAGTGAAGTG TCCCCTGGCA TGGCTTTCAG CAATGTGGCA 
ACCCTTCTCA GTCCGTCTTC CCCAACCACA CACTGGAGTC TGGAGCTCCA GGGACCCACC 
TGTGAACCAG GACCCTCCGA AGAGTCTGAG GCCCCTGCCA GAGGATCTTT GAGAAGATCA 
GCCCCTCCGG AAGGAGCCAA TGGCCTGAGG AGGTTGGATG TAGGCCAAGC AAGGCAGTCG 
GAAAAGCTCA GCCATCCCCA CCTGGAGAAG GCATCCAGCT GGCCCCACAG GCGGGACACA 
GGGAGACCGC TGGAGAGCGT CAGTGGGCGG GTCGCAGATC CCGGTGAGGG GTCCAGTAAG 
CACAAGGGCT GGAACCGGCA AGGCCTGCGC AGACCTTCCA TCTTGCCTGA GGGCTCTTCC 
GATACAAGAG GTCCAGGCGT AGAAAAGGCC CCTGGCCCTT CAGACACCAT TGTGTTTCGG 
GAGAAGAAAC CAAAGGAGGT GATGGGAGGC TTTTCAAGAC GCCGCTCGAA GCTCATCAAC 
TCCTCTCAGC TGCTTTACCA GGAATACAGC GATGTGGTTT TGAACAAGGA GATTCAGAGC 
CAGCAGCGGC TGGACAGCCT GGGAGAGGCC ACGGGGCCCG CCTCCCCCCG GCAGCCCCGG 
AAGGCTCTGG TCTCCTCCGA ATCCTACCTG CAGCGCCTGT CCATGGCCTC CAGTGGCTCC 
CTCTGGCAGG AAATCCCTGT GGTGCGCAAC AGCACCGTGC TGCTCTCCAT GACCCATGAA 
GATCAAAAAC TGCAAGAGGC CAAATTTGAG CTGATCGTGT CAGAGGCCTC ATACCTACGC 
AGTCTGCATG TAGCTGTGGA TCATTTCCAG CTCTCAGCCC CACTGCGGGC CACCCTTTCC 
AACCAGGAAT ACCAGTGGCT CTTCTCTCGT TTACAGGACG TGCGTGAAGT CAGCACCACG 
TTCCTTTCCG ACCTGGAGGA GAACTTCGAG AGCAACATCT TCACCTTCCA AGTGTGTGAC 
GTAGTCCTGA ACCACGCTCC CGACTTTCGC CGGGTCTACC TGCCGTATGT CACCAACCAG 
ACCTACCAGG AGCGCACCTT CCAAGTCCTG CTGAACAGCA ACAGCAGTTT CCGAGAGGTC 
CTGGAGAAGC TGGAGAGCGA CCCCGTCTGC CAGCGCCTCT CCCTGAAGTC CTTTCTGATC 
CTGCCCTTCC AGCGCATCAC GCGTCTCAAA CTGCTGCTCC AGAACATTCT GAAGAGAACA 
CAGCCTGGAT CTCCAGAGGA AGCAGAGGCC ACAAAGGCAC ATCATGCGCT GGAGGAGCTG 
ATCCGAGACT GCAATAACAA TGTCCAGAGG ATGCGACGGA CAGAGGAGCT CATCTACCTG 
AGCCAGAAGA TTGAGTTCGA GTGTAAAATA TTCCCGCTCA TTTCGCAGTC GCGCTGGCTG 
GTGAAGAGTG GAGAGGTGAC AGCCCTGGAG TTCAGTCTGT CCCCAGGGCT GCGAAGGAAG 
CTCAACACGC GTCCTGTCCA CCTGCATCTC TTCAATGACT GTCTGTTGCT GTCTCGGCCC 
CGAGAGGGTA GCCGATTCCT GGTATTTGAC CATGCTCCCT TCTCCTCCAT CCGAGGGGAA 
AAGTGTGAAA TGAAGCTACA CGGACCTCAC AAAAACCTCT TCCGACTCTT CCTGCTGCAC 
AACGCGCAGG GCACCCAGGC CGAGTTCCTC TTCAGCACAG AGACCCAAAG TGAAAAGCTT 
CGATGGATCT CAGCCTTGGC CATGCCCAGA GAGGAGTTGG ACCTTCTGGA GTGTTATGAC 
TCCCCTCAAG TACAGTGCCT TCGAGCCTAC AAACCCCGAG AGAACGACGA GCTGGCACTA 
GAGAAGGCAG ACGTGGTGAT GGTGACCCAG CAAAGCAGCG ATGGCTGGCT GGAGGGCATG 
AGACTCTCAG ATGGGGAGCG AGGCTGGTTC CCTATGCAAC AAGTGGAGTT CATTTCCAAC 
CGAGAGGTCC GTGCCCGGAA CGTGAGTGAA GCCCACCGAG TCAAGACTGC CAAACTACAG 
CTGGTGGAGC AGCAGACCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( XM_005629523.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005629580.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe17928
Clone ID Related Accession (Same CDS sequence)	XM_005629523.1 , XM_539834.5 , XM_005629523.2 , XM_005629524.1 , XM_539834.4
Accession Version	XM_005629523.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629580.1
CDS	253..5067
Translation	MEAEEPQHGALTPIPALAELSIIPEPLRRSSQTATLGPEAQEGWEPSSTWAEGRGLLEPQQGGLKDVKSCATESMTSFPEEAPAGVETDQEDTVGDTWDTPERQEPVPQSLADGRARTPAHAERGACPFQGEHLDTVPVSRERGGGVEMELRPELTSLPGGTGHAEEEEVSPDTSAQPRSGPPCEEHPAEPELPGDSVKQGEELRAAGAEESPGRGGLMCLLDAWGLEEQGLAEAAFREEAAAGGDGRLGGQEQGGEQADGTGEQEQKQEQRRGRAMCAKHGERTGLSGELEGLRCSEQGMEPRRQPGLQQGEEGNRETGGPEEGSGAAQNEEKWSVVGKSGQVTGRPEGRGLRGLRGKVASVEGREAEAVGSGEEEPENWDGDPLRGEGREGPTEEGEARDGPSTLALVASEVSSPCDLFPDTCYPMTGTPGPREEPRAEEPSPVALPPPLESGGWSGRPASLPGSFPAGGSPAQEAAQDSQQEGPELGKRAVFGQGTQMVSAGTPATPPRTPHPPPSGPAEVSSRTTTSLSASPPVGSPRREPFLAAHSPETSRATLRTTHAHHCGASETPPAALPGASSAEATVETPTSSIWAHPQGPPGHPAGAVQHLRSNSFPGSHRTEPTPDLLGLSLSFSHSELPQRAPKPAIYGSVIPRRDRKSGRDCRIIPESPSSLSTLGWDSQEFTSNLERSSSPCSTQPCGSPHTSASAPESPAYGSSPPFISVDGRIPEPLPPPPPEKRHVHPSVVERDSHLHAGVATLKRCGHALPLAPGSGLHGPPKGPLPPFPDPLVARQHRPLPSTPDTPTPTLTSFSRRLRYNKPLPPTPDLSQPHHSVSFSNPRIYRPLPPVPIMDPPIEPPPLPPKSKGRSRSTQGELMNSGGRGKPRPVCQERTVSAPHSVGRTSWPPAMGRSTDSLASTSRSKSEVSPGMAFSNVATLLSPSSPTTHWSLELQGPTCEPGPSEESEAPARGSLRRSAPPEGANGLRRLDVGQARQSEKLSHPHLEKASSWPHRRDTGRPLESVSGRVADPGEGSSKHKGWNRQGLRRPSILPEGSSDTRGPGVEKAPGPSDTIVFREKKPKEVMGGFSRRRSKLINSSQLLYQEYSDVVLNKEIQSQQRLDSLGEATGPASPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLHVAVDHFQLSAPLRATLSNQEYQWLFSRLQDVREVSTTFLSDLEENFESNIFTFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQVLLNSNSSFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSPEEAEATKAHHALEELIRDCNNNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGEVTALEFSLSPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQAEFLFSTETQSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGMRLSDGERGWFPMQQVEFISNREVRARNVSEAHRVKTAKLQLVEQQT

Target ORF information:

RefSeq Version	XM_005629523.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629523.1

ORF Insert Sequence:

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( XM_539834.5 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_539834.3 Canis lupus familiaris Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe17928
Clone ID Related Accession (Same CDS sequence)	XM_005629523.1 , XM_539834.5 , XM_005629523.2 , XM_005629524.1 , XM_539834.4
Accession Version	XM_539834.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_539834.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539834.3
CDS	117..4931
Translation	MEAEEPQHGALTPIPALAELSIIPEPLRRSSQTATLGPEAQEGWEPSSTWAEGRGLLEPQQGGLKDVKSCATESMTSFPEEAPAGVETDQEDTVGDTWDTPERQEPVPQSLADGRARTPAHAERGACPFQGEHLDTVPVSRERGGGVEMELRPELTSLPGGTGHAEEEEVSPDTSAQPRSGPPCEEHPAEPELPGDSVKQGEELRAAGAEESPGRGGLMCLLDAWGLEEQGLAEAAFREEAAAGGDGRLGGQEQGGEQADGTGEQEQKQEQRRGRAMCAKHGERTGLSGELEGLRCSEQGMEPRRQPGLQQGEEGNRETGGPEEGSGAAQNEEKWSVVGKSGQVTGRPEGRGLRGLRGKVASVEGREAEAVGSGEEEPENWDGDPLRGEGREGPTEEGEARDGPSTLALVASEVSSPCDLFPDTCYPMTGTPGPREEPRAEEPSPVALPPPLESGGWSGRPASLPGSFPAGGSPAQEAAQDSQQEGPELGKRAVFGQGTQMVSAGTPATPPRTPHPPPSGPAEVSSRTTTSLSASPPVGSPRREPFLAAHSPETSRATLRTTHAHHCGASETPPAALPGASSAEATVETPTSSIWAHPQGPPGHPAGAVQHLRSNSFPGSHRTEPTPDLLGLSLSFSHSELPQRAPKPAIYGSVIPRRDRKSGRDCRIIPESPSSLSTLGWDSQEFTSNLERSSSPCSTQPCGSPHTSASAPESPAYGSSPPFISVDGRIPEPLPPPPPEKRHVHPSVVERDSHLHAGVATLKRCGHALPLAPGSGLHGPPKGPLPPFPDPLVARQHRPLPSTPDTPTPTLTSFSRRLRYNKPLPPTPDLSQPHHSVSFSNPRIYRPLPPVPIMDPPIEPPPLPPKSKGRSRSTQGELMNSGGRGKPRPVCQERTVSAPHSVGRTSWPPAMGRSTDSLASTSRSKSEVSPGMAFSNVATLLSPSSPTTHWSLELQGPTCEPGPSEESEAPARGSLRRSAPPEGANGLRRLDVGQARQSEKLSHPHLEKASSWPHRRDTGRPLESVSGRVADPGEGSSKHKGWNRQGLRRPSILPEGSSDTRGPGVEKAPGPSDTIVFREKKPKEVMGGFSRRRSKLINSSQLLYQEYSDVVLNKEIQSQQRLDSLGEATGPASPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLHVAVDHFQLSAPLRATLSNQEYQWLFSRLQDVREVSTTFLSDLEENFESNIFTFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQVLLNSNSSFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSPEEAEATKAHHALEELIRDCNNNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGEVTALEFSLSPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQAEFLFSTETQSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGMRLSDGERGWFPMQQVEFISNREVRARNVSEAHRVKTAKLQLVEQQT

Target ORF information:

RefSeq Version	XM_539834.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539834.5

ORF Insert Sequence:

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( XM_005629523.2 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005629580.1 Canis lupus familiaris Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X2, mRNA.

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CloneID	OCe17928
Clone ID Related Accession (Same CDS sequence)	XM_005629523.1 , XM_539834.5 , XM_005629523.2 , XM_005629524.1 , XM_539834.4
Accession Version	XM_005629523.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	rho guanine nucleotide exchange factor 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005629523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629580.1
CDS	213..5027
Translation	MEAEEPQHGALTPIPALAELSIIPEPLRRSSQTATLGPEAQEGWEPSSTWAEGRGLLEPQQGGLKDVKSCATESMTSFPEEAPAGVETDQEDTVGDTWDTPERQEPVPQSLADGRARTPAHAERGACPFQGEHLDTVPVSRERGGGVEMELRPELTSLPGGTGHAEEEEVSPDTSAQPRSGPPCEEHPAEPELPGDSVKQGEELRAAGAEESPGRGGLMCLLDAWGLEEQGLAEAAFREEAAAGGDGRLGGQEQGGEQADGTGEQEQKQEQRRGRAMCAKHGERTGLSGELEGLRCSEQGMEPRRQPGLQQGEEGNRETGGPEEGSGAAQNEEKWSVVGKSGQVTGRPEGRGLRGLRGKVASVEGREAEAVGSGEEEPENWDGDPLRGEGREGPTEEGEARDGPSTLALVASEVSSPCDLFPDTCYPMTGTPGPREEPRAEEPSPVALPPPLESGGWSGRPASLPGSFPAGGSPAQEAAQDSQQEGPELGKRAVFGQGTQMVSAGTPATPPRTPHPPPSGPAEVSSRTTTSLSASPPVGSPRREPFLAAHSPETSRATLRTTHAHHCGASETPPAALPGASSAEATVETPTSSIWAHPQGPPGHPAGAVQHLRSNSFPGSHRTEPTPDLLGLSLSFSHSELPQRAPKPAIYGSVIPRRDRKSGRDCRIIPESPSSLSTLGWDSQEFTSNLERSSSPCSTQPCGSPHTSASAPESPAYGSSPPFISVDGRIPEPLPPPPPEKRHVHPSVVERDSHLHAGVATLKRCGHALPLAPGSGLHGPPKGPLPPFPDPLVARQHRPLPSTPDTPTPTLTSFSRRLRYNKPLPPTPDLSQPHHSVSFSNPRIYRPLPPVPIMDPPIEPPPLPPKSKGRSRSTQGELMNSGGRGKPRPVCQERTVSAPHSVGRTSWPPAMGRSTDSLASTSRSKSEVSPGMAFSNVATLLSPSSPTTHWSLELQGPTCEPGPSEESEAPARGSLRRSAPPEGANGLRRLDVGQARQSEKLSHPHLEKASSWPHRRDTGRPLESVSGRVADPGEGSSKHKGWNRQGLRRPSILPEGSSDTRGPGVEKAPGPSDTIVFREKKPKEVMGGFSRRRSKLINSSQLLYQEYSDVVLNKEIQSQQRLDSLGEATGPASPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLHVAVDHFQLSAPLRATLSNQEYQWLFSRLQDVREVSTTFLSDLEENFESNIFTFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQVLLNSNSSFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSPEEAEATKAHHALEELIRDCNNNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGEVTALEFSLSPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQAEFLFSTETQSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGMRLSDGERGWFPMQQVEFISNREVRARNVSEAHRVKTAKLQLVEQQT

Target ORF information:

RefSeq Version	XM_005629523.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor 5 (ARHGEF5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629523.2

ORF Insert Sequence:

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( XM_005629524.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_005629581.1 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X3, mRNA.

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CloneID	OCe17928
Clone ID Related Accession (Same CDS sequence)	XM_005629523.1 , XM_539834.5 , XM_005629523.2 , XM_005629524.1 , XM_539834.4
Accession Version	XM_005629524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005629581.1
CDS	24..4838
Translation	MEAEEPQHGALTPIPALAELSIIPEPLRRSSQTATLGPEAQEGWEPSSTWAEGRGLLEPQQGGLKDVKSCATESMTSFPEEAPAGVETDQEDTVGDTWDTPERQEPVPQSLADGRARTPAHAERGACPFQGEHLDTVPVSRERGGGVEMELRPELTSLPGGTGHAEEEEVSPDTSAQPRSGPPCEEHPAEPELPGDSVKQGEELRAAGAEESPGRGGLMCLLDAWGLEEQGLAEAAFREEAAAGGDGRLGGQEQGGEQADGTGEQEQKQEQRRGRAMCAKHGERTGLSGELEGLRCSEQGMEPRRQPGLQQGEEGNRETGGPEEGSGAAQNEEKWSVVGKSGQVTGRPEGRGLRGLRGKVASVEGREAEAVGSGEEEPENWDGDPLRGEGREGPTEEGEARDGPSTLALVASEVSSPCDLFPDTCYPMTGTPGPREEPRAEEPSPVALPPPLESGGWSGRPASLPGSFPAGGSPAQEAAQDSQQEGPELGKRAVFGQGTQMVSAGTPATPPRTPHPPPSGPAEVSSRTTTSLSASPPVGSPRREPFLAAHSPETSRATLRTTHAHHCGASETPPAALPGASSAEATVETPTSSIWAHPQGPPGHPAGAVQHLRSNSFPGSHRTEPTPDLLGLSLSFSHSELPQRAPKPAIYGSVIPRRDRKSGRDCRIIPESPSSLSTLGWDSQEFTSNLERSSSPCSTQPCGSPHTSASAPESPAYGSSPPFISVDGRIPEPLPPPPPEKRHVHPSVVERDSHLHAGVATLKRCGHALPLAPGSGLHGPPKGPLPPFPDPLVARQHRPLPSTPDTPTPTLTSFSRRLRYNKPLPPTPDLSQPHHSVSFSNPRIYRPLPPVPIMDPPIEPPPLPPKSKGRSRSTQGELMNSGGRGKPRPVCQERTVSAPHSVGRTSWPPAMGRSTDSLASTSRSKSEVSPGMAFSNVATLLSPSSPTTHWSLELQGPTCEPGPSEESEAPARGSLRRSAPPEGANGLRRLDVGQARQSEKLSHPHLEKASSWPHRRDTGRPLESVSGRVADPGEGSSKHKGWNRQGLRRPSILPEGSSDTRGPGVEKAPGPSDTIVFREKKPKEVMGGFSRRRSKLINSSQLLYQEYSDVVLNKEIQSQQRLDSLGEATGPASPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLHVAVDHFQLSAPLRATLSNQEYQWLFSRLQDVREVSTTFLSDLEENFESNIFTFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQVLLNSNSSFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSPEEAEATKAHHALEELIRDCNNNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGEVTALEFSLSPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQAEFLFSTETQSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGMRLSDGERGWFPMQQVEFISNREVRARNVSEAHRVKTAKLQLVEQQT

Target ORF information:

RefSeq Version	XM_005629524.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005629524.1

ORF Insert Sequence:

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF5 ( XM_539834.4 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_539834.3 Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X1, mRNA.

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CloneID	OCe17928
Clone ID Related Accession (Same CDS sequence)	XM_005629523.1 , XM_539834.5 , XM_005629523.2 , XM_005629524.1 , XM_539834.4
Accession Version	XM_539834.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	rho guanine nucleotide exchange factor 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2013 this sequence version replaced XM_539834.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_539834.3
CDS	152..4966
Translation	MEAEEPQHGALTPIPALAELSIIPEPLRRSSQTATLGPEAQEGWEPSSTWAEGRGLLEPQQGGLKDVKSCATESMTSFPEEAPAGVETDQEDTVGDTWDTPERQEPVPQSLADGRARTPAHAERGACPFQGEHLDTVPVSRERGGGVEMELRPELTSLPGGTGHAEEEEVSPDTSAQPRSGPPCEEHPAEPELPGDSVKQGEELRAAGAEESPGRGGLMCLLDAWGLEEQGLAEAAFREEAAAGGDGRLGGQEQGGEQADGTGEQEQKQEQRRGRAMCAKHGERTGLSGELEGLRCSEQGMEPRRQPGLQQGEEGNRETGGPEEGSGAAQNEEKWSVVGKSGQVTGRPEGRGLRGLRGKVASVEGREAEAVGSGEEEPENWDGDPLRGEGREGPTEEGEARDGPSTLALVASEVSSPCDLFPDTCYPMTGTPGPREEPRAEEPSPVALPPPLESGGWSGRPASLPGSFPAGGSPAQEAAQDSQQEGPELGKRAVFGQGTQMVSAGTPATPPRTPHPPPSGPAEVSSRTTTSLSASPPVGSPRREPFLAAHSPETSRATLRTTHAHHCGASETPPAALPGASSAEATVETPTSSIWAHPQGPPGHPAGAVQHLRSNSFPGSHRTEPTPDLLGLSLSFSHSELPQRAPKPAIYGSVIPRRDRKSGRDCRIIPESPSSLSTLGWDSQEFTSNLERSSSPCSTQPCGSPHTSASAPESPAYGSSPPFISVDGRIPEPLPPPPPEKRHVHPSVVERDSHLHAGVATLKRCGHALPLAPGSGLHGPPKGPLPPFPDPLVARQHRPLPSTPDTPTPTLTSFSRRLRYNKPLPPTPDLSQPHHSVSFSNPRIYRPLPPVPIMDPPIEPPPLPPKSKGRSRSTQGELMNSGGRGKPRPVCQERTVSAPHSVGRTSWPPAMGRSTDSLASTSRSKSEVSPGMAFSNVATLLSPSSPTTHWSLELQGPTCEPGPSEESEAPARGSLRRSAPPEGANGLRRLDVGQARQSEKLSHPHLEKASSWPHRRDTGRPLESVSGRVADPGEGSSKHKGWNRQGLRRPSILPEGSSDTRGPGVEKAPGPSDTIVFREKKPKEVMGGFSRRRSKLINSSQLLYQEYSDVVLNKEIQSQQRLDSLGEATGPASPRQPRKALVSSESYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLHVAVDHFQLSAPLRATLSNQEYQWLFSRLQDVREVSTTFLSDLEENFESNIFTFQVCDVVLNHAPDFRRVYLPYVTNQTYQERTFQVLLNSNSSFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKRTQPGSPEEAEATKAHHALEELIRDCNNNVQRMRRTEELIYLSQKIEFECKIFPLISQSRWLVKSGEVTALEFSLSPGLRRKLNTRPVHLHLFNDCLLLSRPREGSRFLVFDHAPFSSIRGEKCEMKLHGPHKNLFRLFLLHNAQGTQAEFLFSTETQSEKLRWISALAMPREELDLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGMRLSDGERGWFPMQQVEFISNREVRARNVSEAHRVKTAKLQLVEQQT

Target ORF information:

RefSeq Version	XM_539834.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Rho guanine nucleotide exchange factor (GEF) 5 (ARHGEF5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_539834.4

ORF Insert Sequence:

ATGGAGGCTG AGGAGCCCCA ACACGGGGCC TTGACCCCCA TCCCTGCCTT AGCAGAGCTC 
AGCATTATCC CGGAACCTCT CAGGAGAAGC AGTCAGACTG CCACCTTGGG GCCCGAAGCT 
CAAGAAGGCT GGGAGCCATC GTCCACATGG GCAGAGGGAC GAGGACTCTT GGAGCCCCAG 
CAGGGAGGCC TAAAGGATGT GAAGAGCTGT GCAACTGAGA GCATGACCTC CTTCCCCGAG 
GAAGCCCCTG CAGGTGTGGA GACCGACCAG GAAGACACCG TGGGTGACAC GTGGGACACC 
CCGGAGCGCC AGGAGCCGGT GCCTCAGAGC CTGGCAGACG GTCGGGCAAG GACACCAGCT 
CACGCAGAGC GCGGGGCCTG CCCTTTTCAG GGTGAACATC TAGACACGGT CCCAGTGTCC 
AGAGAGCGGG GTGGCGGGGT GGAGATGGAG CTTAGACCAG AGCTCACTTC CTTGCCTGGG 
GGAACTGGAC ATGCAGAAGA GGAGGAGGTT TCTCCAGACA CCTCCGCTCA GCCCCGATCT 
GGGCCTCCCT GTGAAGAGCA CCCTGCAGAG CCCGAGCTCC CGGGGGACTC TGTCAAGCAG 
GGGGAAGAGC TGCGGGCTGC GGGGGCCGAA GAAAGTCCAG GGAGAGGGGG ACTCATGTGC 
CTTCTGGATG CCTGGGGGCT GGAGGAGCAG GGACTGGCCG AAGCGGCCTT CCGGGAGGAA 
GCAGCGGCGG GGGGCGATGG GCGTTTAGGG GGACAGGAAC AGGGCGGGGA GCAGGCGGAC 
GGTACAGGAG AGCAAGAGCA GAAACAGGAG CAGAGACGAG GCCGTGCGAT GTGTGCAAAG 
CACGGAGAGA GAACGGGGCT TTCTGGGGAA TTGGAAGGTC TGCGCTGCAG TGAGCAGGGG 
ATGGAGCCTC GGCGCCAGCC GGGTCTACAG CAGGGAGAGG AGGGGAACAG GGAGACAGGG 
GGGCCAGAGG AGGGGAGCGG GGCTGCTCAG AATGAGGAGA AGTGGAGTGT GGTGGGCAAA 
TCAGGGCAAG TAACTGGAAG GCCAGAAGGT CGAGGTCTTC GAGGTCTTCG GGGGAAGGTG 
GCCTCGGTGG AGGGGCGGGA GGCGGAGGCA GTGGGGTCAG GGGAGGAGGA GCCGGAGAAT 
TGGGATGGGG ACCCGCTTCG GGGAGAGGGC AGAGAGGGGC CTACAGAAGA GGGGGAAGCA 
CGTGATGGTC CTTCCACACT GGCTCTGGTA GCCTCTGAGG TCTCTTCTCC CTGTGACTTG 
TTTCCAGACA CCTGTTATCC CATGACCGGT ACTCCTGGGC CTCGGGAGGA GCCTAGGGCT 
GAGGAACCAT CCCCTGTAGC TCTGCCTCCT CCACTGGAGT CCGGAGGGTG GTCGGGCCGG 
CCTGCTTCTT TACCTGGTTC CTTTCCTGCT GGGGGGTCGC CCGCCCAAGA AGCAGCTCAG 
GACAGCCAGC AGGAGGGACC CGAGCTGGGG AAGAGGGCAG TGTTCGGCCA GGGGACCCAG 
ATGGTCTCTG CCGGTACCCC TGCAACTCCT CCGAGGACTC CCCATCCACC TCCTTCCGGT 
CCTGCTGAAG TCTCCTCCCG GACGACTACA TCATTGTCTG CCAGCCCCCC AGTTGGCTCT 
CCCAGGAGGG AGCCCTTTCT TGCTGCGCAT TCTCCAGAAA CCTCCAGAGC AACTCTTCGG 
ACCACCCACG CACATCACTG TGGGGCTTCC GAGACTCCCC CAGCTGCCCT CCCTGGCGCT 
TCCTCTGCAG AGGCCACCGT GGAAACACCC ACCAGCTCCA TCTGGGCCCA CCCCCAGGGC 
CCCCCAGGCC ACCCTGCAGG GGCTGTCCAG CACCTAAGGA GCAACTCCTT CCCAGGCTCT 
CATAGGACAG AGCCAACTCC GGACCTGCTG GGACTCTCAC TTTCCTTTTC TCATTCAGAG 
TTGCCCCAAA GGGCCCCCAA ACCTGCCATC TATGGCTCTG TGATCCCAAG AAGGGACAGG 
AAAAGTGGTA GGGACTGTAG GATCATTCCC GAATCCCCTA GTTCATTATC TACTCTGGGG 
TGGGACTCTC AAGAATTCAC TTCAAATCTG GAAAGATCCA GTAGTCCCTG TAGCACCCAG 
CCATGTGGCT CCCCACACAC TTCAGCCTCT GCCCCAGAGT CTCCTGCCTA TGGCTCTTCC 
CCACCCTTTA TCTCTGTAGA TGGGAGGATC CCTGAACCTC TGCCCCCTCC TCCCCCAGAG 
AAGAGGCATG TCCACCCCTC CGTGGTAGAG AGAGACAGCC ACCTCCATGC AGGAGTTGCC 
ACGTTGAAGA GGTGTGGCCA TGCTCTTCCA TTGGCCCCAG GTTCGGGGCT ACATGGTCCC 
CCCAAAGGCC CACTTCCCCC ATTTCCTGAC CCCCTCGTGG CAAGGCAGCA TCGACCTCTG 
CCCTCTACCC CGGACACTCC CACCCCTACT CTGACCTCTT TCTCCCGTAG GCTGAGATAC 
AATAAGCCAT TGCCCCCAAC TCCTGATTTG TCCCAGCCCC ACCATTCTGT TTCTTTTAGT 
AATCCAAGGA TCTACAGGCC TCTCCCCCCT GTCCCTATTA TGGATCCTCC CATTGAACCA 
CCTCCGTTAC CCCCCAAGTC CAAGGGGAGG AGTAGGAGCA CTCAGGGAGA ACTCATGAAT 
TCAGGGGGTC GGGGTAAGCC AAGGCCTGTT TGTCAAGAGC GGACAGTCTC TGCTCCCCAT 
TCTGTTGGAC GTACCTCCTG GCCCCCAGCC ATGGGCCGAT CAACAGACTC TTTGGCTTCT 
ACCAGCAGGA GTAAGAGTGA AGTGTCCCCT GGCATGGCTT TCAGCAATGT GGCAACCCTT 
CTCAGTCCGT CTTCCCCAAC CACACACTGG AGTCTGGAGC TCCAGGGACC CACCTGTGAA 
CCAGGACCCT CCGAAGAGTC TGAGGCCCCT GCCAGAGGAT CTTTGAGAAG ATCAGCCCCT 
CCGGAAGGAG CCAATGGCCT GAGGAGGTTG GATGTAGGCC AAGCAAGGCA GTCGGAAAAG 
CTCAGCCATC CCCACCTGGA GAAGGCATCC AGCTGGCCCC ACAGGCGGGA CACAGGGAGA 
CCGCTGGAGA GCGTCAGTGG GCGGGTCGCA GATCCCGGTG AGGGGTCCAG TAAGCACAAG 
GGCTGGAACC GGCAAGGCCT GCGCAGACCT TCCATCTTGC CTGAGGGCTC TTCCGATACA 
AGAGGTCCAG GCGTAGAAAA GGCCCCTGGC CCTTCAGACA CCATTGTGTT TCGGGAGAAG 
AAACCAAAGG AGGTGATGGG AGGCTTTTCA AGACGCCGCT CGAAGCTCAT CAACTCCTCT 
CAGCTGCTTT ACCAGGAATA CAGCGATGTG GTTTTGAACA AGGAGATTCA GAGCCAGCAG 
CGGCTGGACA GCCTGGGAGA GGCCACGGGG CCCGCCTCCC CCCGGCAGCC CCGGAAGGCT 
CTGGTCTCCT CCGAATCCTA CCTGCAGCGC CTGTCCATGG CCTCCAGTGG CTCCCTCTGG 
CAGGAAATCC CTGTGGTGCG CAACAGCACC GTGCTGCTCT CCATGACCCA TGAAGATCAA 
AAACTGCAAG AGGCCAAATT TGAGCTGATC GTGTCAGAGG CCTCATACCT ACGCAGTCTG 
CATGTAGCTG TGGATCATTT CCAGCTCTCA GCCCCACTGC GGGCCACCCT TTCCAACCAG 
GAATACCAGT GGCTCTTCTC TCGTTTACAG GACGTGCGTG AAGTCAGCAC CACGTTCCTT 
TCCGACCTGG AGGAGAACTT CGAGAGCAAC ATCTTCACCT TCCAAGTGTG TGACGTAGTC 
CTGAACCACG CTCCCGACTT TCGCCGGGTC TACCTGCCGT ATGTCACCAA CCAGACCTAC 
CAGGAGCGCA CCTTCCAAGT CCTGCTGAAC AGCAACAGCA GTTTCCGAGA GGTCCTGGAG 
AAGCTGGAGA GCGACCCCGT CTGCCAGCGC CTCTCCCTGA AGTCCTTTCT GATCCTGCCC 
TTCCAGCGCA TCACGCGTCT CAAACTGCTG CTCCAGAACA TTCTGAAGAG AACACAGCCT 
GGATCTCCAG AGGAAGCAGA GGCCACAAAG GCACATCATG CGCTGGAGGA GCTGATCCGA 
GACTGCAATA ACAATGTCCA GAGGATGCGA CGGACAGAGG AGCTCATCTA CCTGAGCCAG 
AAGATTGAGT TCGAGTGTAA AATATTCCCG CTCATTTCGC AGTCGCGCTG GCTGGTGAAG 
AGTGGAGAGG TGACAGCCCT GGAGTTCAGT CTGTCCCCAG GGCTGCGAAG GAAGCTCAAC 
ACGCGTCCTG TCCACCTGCA TCTCTTCAAT GACTGTCTGT TGCTGTCTCG GCCCCGAGAG 
GGTAGCCGAT TCCTGGTATT TGACCATGCT CCCTTCTCCT CCATCCGAGG GGAAAAGTGT 
GAAATGAAGC TACACGGACC TCACAAAAAC CTCTTCCGAC TCTTCCTGCT GCACAACGCG 
CAGGGCACCC AGGCCGAGTT CCTCTTCAGC ACAGAGACCC AAAGTGAAAA GCTTCGATGG 
ATCTCAGCCT TGGCCATGCC CAGAGAGGAG TTGGACCTTC TGGAGTGTTA TGACTCCCCT 
CAAGTACAGT GCCTTCGAGC CTACAAACCC CGAGAGAACG ACGAGCTGGC ACTAGAGAAG 
GCAGACGTGG TGATGGTGAC CCAGCAAAGC AGCGATGGCT GGCTGGAGGG CATGAGACTC 
TCAGATGGGG AGCGAGGCTG GTTCCCTATG CAACAAGTGG AGTTCATTTC CAACCGAGAG 
GTCCGTGCCC GGAACGTGAG TGAAGCCCAC CGAGTCAAGA CTGCCAAACT ACAGCTGGTG 
GAGCAGCAGA CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

ARHGEF5 cDNA ORF clone, Canis lupus familiaris(Dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム