SLC35B2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC35B2
Entrez Gene ID	481819
Full Name	solute carrier family 35 member B2
Synonyms	PAPST1
General protein information	Preferred Names solute carrier family 35 member B2 Names adenosine 3'-phospho 5'-phosphosulfate transporter 1 3'-phosphoadenosine 5'-phosphosulfate transporter 1
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001127499.1	NP_001120971.1	adenosine 3'-phospho 5'-phosphosulfate transporter 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC35B2	NP_835361.1
Mus musculus (house mouse)	Slc35b2	NP_082938.1
Gallus gallus (chicken)	SLC35B2	XP_426131.2
Canis lupus familiaris (dog)	SLC35B2	NP_001120971.1
Danio rerio (zebrafish)	slc35b2	NP_991198.1
	LOC703388	XP_002803843.1
Rattus norvegicus (Norway rat)	Slc35b2	NP_001032292.1
Arabidopsis thaliana (thale cress)	UTR5	NP_190204.2
Arabidopsis thaliana (thale cress)	AT5G59740	NP_200782.1
Bos taurus (cattle)	SLC35B2	NP_001070433.1
Drosophila melanogaster (fruit fly)	sll	NP_524389.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002571	XP_312365.4
Caenorhabditis elegans (roundworm)	pst-1	NP_741545.2
Oryza sativa (rice)	Os05g0115700	NP_001054466.1
Xenopus tropicalis (tropical clawed frog)	slc35b2	NP_001182701.1

Gene Ontology
Bibliography

Related articles in PubMed

PAPST1 regulates sulfation of heparan sulfate proteoglycans in epithelial MDCK II cells.
Dick G, Akslen-Hoel LK, Gr?ndahl F, Kjos I, Maccarana M, Prydz K
Glycobiology25(1)30-41(2015 Jan)

Overexpression of the 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transporter 1 increases sulfation of chondroitin sulfate in the apical pathway of MDCK II cells.
Dick G, Gr?ndahl F, Prydz K
Glycobiology18(1)53-65(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Regulation of PAPST1 allows epithelial cells to prioritize between proteoglycan sulfation in the apical and basolateral secretory routes at the level of the Golgi apparatus.
Title: PAPST1 regulates sulfation of heparan sulfate proteoglycans in epithelial MDCK II cells.

The canine 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transporter 1 fused to GFP was stably expressed with a typical Golgi localization in MDCK II cells
Title: Overexpression of the 3'-phosphoadenosine 5'-phosphosulfate (PAPS) transporter 1 increases sulfation of chondroitin sulfate in the apical pathway of MDCK II cells.

The following SLC35B2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC35B2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe34050	NM_001127499.1 Latest version!	Canis lupus familiaris solute carrier family 35 member B2 (SLC35B2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC35B2 ( NM_001127499.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001120971.1 Canis lupus familiaris solute carrier family 35 member B2 (SLC35B2), mRNA.

Price & Availability↑

CloneID	OCe34050
Clone ID Related Accession (Same CDS sequence)	NM_001127499.1
Accession Version	NM_001127499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Canis lupus familiaris(dog)
Product	adenosine 3'-phospho 5'-phosphosulfate transporter 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EF568109.1. On May 22, 2008 this sequence version replaced XM_538940.2. ##Evidence-Data-START## Transcript exon combination :: EF568109.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END##

ATGGACGCCA GGTGGTGGGC AGTGGTGGTG CTGGCTGCGC TCCCCTCCCT GGGGGCAGGG 
GGCGAGACCC CCGAAGCCCT TCCAGAGTCA TGGACGCAGC TGTGGTTCTT CCGCTTTTTG 
GTGAACGCTG CTGGCTATGC CAGCTTTATG GTCCCCGGCT ACCTGATGGT GCAGTACTTC 
AGGCGGAAGA ACTACCTGGA GACAGGCAGG GGTCTCTGCT TCCCCCTGGT GAAAACTTGT 
GTGTTTGGCA ATGAGCCCAA GGCCTCTGAC GAGGTCCCTC TGACTTCGCG GTCTGAGCCT 
GCGGAGACCA CTCCCACTTG GCAAGCCCTG AAGCTGCTCT TCTGTGCTGC GGGGCTCCAG 
GTGTCTTATC TGACCTGGGG AGTGCTGCAG GAAAGAGTGA TGACCCGCAG CTATGGGGCT 
ACTGCTACAT CGCCAGGTGA GCGCTTCACA GACTCACAGT TCCTGGTGCT AATGAACCGA 
GTGCTGGCGC TGATCGTAGC TGGCCTCTAC TGCGTCCTCT GCAAGCAGCC CCGCCACGGG 
GCGCCCATGT ACCGGTACTC CTTTGCCAGC CTGTCAAACG TGCTTAGCAG CTGGTGTCAG 
TACGAAGCCC TCAAGTTCGT CAGCTTCCCC ACCCAGGTGC TGGCCAAGGC CTCTAAGGTG 
ATCCCAGTCA TGCTCATGGG AAAGCTGGTG TCGCGGCGCA GCTACGAACA CTGGGAATAC 
CTGACGGCTG GCCTCATCTC CATTGGGGTC AGCATGTTTC TGCTGTCCAG TGGACCAGAG 
CCCCGCAGCT CCCCAGCCAC CACGCTGTCA GGCCTCATCC TCCTGGCAGG CTATATTGCC 
TTTGACAGCT TCACCTCAAA CTGGCAGGAT GCCCTGTTTG CCTATAAGAT GTCTTCGGTG 
CAGATGATGT TCGGGGTCAA CTTATTCTCC TGCCTCTTCA CCGTGGGCTC GCTGCTAGAG 
CAGGGGGCCC TCCTGGAGGG GACCCGCTTC ATGGGGCGAC ACAGTGAATT TGCTGCACAT 
GCCCTGCTGC TCTCTATCTG CTCTGCGTGT GGCCAGCTCT TCATCTTCTA CACCATAGGG 
CAGTTTGGGG CTGCCGTCTT CACCATCATC ATGACCCTCC GCCAGGCCTT TGCCATCCTC 
CTCTCCTGCC TTCTCTATGG CCACACTGTC ACTGTGGTGG GGGGCCTGGG GGTGGCTGTG 
GTCTTTGCAG CCCTCCTGCT CAGAGTCTAT GCCCGGGGCC GTCTAAAGCA ACGGGGAAAG 
AAGGCTGTGC CTGTGGAGTC CCCTGTGCAA AAAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120971.1
CDS	1..1299
Translation	MDARWWAVVVLAALPSLGAGGETPEALPESWTQLWFFRFLVNAAGYASFMVPGYLMVQYFRRKNYLETGRGLCFPLVKTCVFGNEPKASDEVPLTSRSEPAETTPTWQALKLLFCAAGLQVSYLTWGVLQERVMTRSYGATATSPGERFTDSQFLVLMNRVLALIVAGLYCVLCKQPRHGAPMYRYSFASLSNVLSSWCQYEALKFVSFPTQVLAKASKVIPVMLMGKLVSRRSYEHWEYLTAGLISIGVSMFLLSSGPEPRSSPATTLSGLILLAGYIAFDSFTSNWQDALFAYKMSSVQMMFGVNLFSCLFTVGSLLEQGALLEGTRFMGRHSEFAAHALLLSICSACGQLFIFYTIGQFGAAVFTIIMTLRQAFAILLSCLLYGHTVTVVGGLGVAVVFAALLLRVYARGRLKQRGKKAVPVESPVQKV

Target ORF information:

RefSeq Version	NM_001127499.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris solute carrier family 35 member B2 (SLC35B2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127499.1

ORF Insert Sequence:

ATGGACGCCA GGTGGTGGGC AGTGGTGGTG CTGGCTGCGC TCCCCTCCCT GGGGGCAGGG 
GGCGAGACCC CCGAAGCCCT TCCAGAGTCA TGGACGCAGC TGTGGTTCTT CCGCTTTTTG 
GTGAACGCTG CTGGCTATGC CAGCTTTATG GTCCCCGGCT ACCTGATGGT GCAGTACTTC 
AGGCGGAAGA ACTACCTGGA GACAGGCAGG GGTCTCTGCT TCCCCCTGGT GAAAACTTGT 
GTGTTTGGCA ATGAGCCCAA GGCCTCTGAC GAGGTCCCTC TGACTTCGCG GTCTGAGCCT 
GCGGAGACCA CTCCCACTTG GCAAGCCCTG AAGCTGCTCT TCTGTGCTGC GGGGCTCCAG 
GTGTCTTATC TGACCTGGGG AGTGCTGCAG GAAAGAGTGA TGACCCGCAG CTATGGGGCT 
ACTGCTACAT CGCCAGGTGA GCGCTTCACA GACTCACAGT TCCTGGTGCT AATGAACCGA 
GTGCTGGCGC TGATCGTAGC TGGCCTCTAC TGCGTCCTCT GCAAGCAGCC CCGCCACGGG 
GCGCCCATGT ACCGGTACTC CTTTGCCAGC CTGTCAAACG TGCTTAGCAG CTGGTGTCAG 
TACGAAGCCC TCAAGTTCGT CAGCTTCCCC ACCCAGGTGC TGGCCAAGGC CTCTAAGGTG 
ATCCCAGTCA TGCTCATGGG AAAGCTGGTG TCGCGGCGCA GCTACGAACA CTGGGAATAC 
CTGACGGCTG GCCTCATCTC CATTGGGGTC AGCATGTTTC TGCTGTCCAG TGGACCAGAG 
CCCCGCAGCT CCCCAGCCAC CACGCTGTCA GGCCTCATCC TCCTGGCAGG CTATATTGCC 
TTTGACAGCT TCACCTCAAA CTGGCAGGAT GCCCTGTTTG CCTATAAGAT GTCTTCGGTG 
CAGATGATGT TCGGGGTCAA CTTATTCTCC TGCCTCTTCA CCGTGGGCTC GCTGCTAGAG 
CAGGGGGCCC TCCTGGAGGG GACCCGCTTC ATGGGGCGAC ACAGTGAATT TGCTGCACAT 
GCCCTGCTGC TCTCTATCTG CTCTGCGTGT GGCCAGCTCT TCATCTTCTA CACCATAGGG 
CAGTTTGGGG CTGCCGTCTT CACCATCATC ATGACCCTCC GCCAGGCCTT TGCCATCCTC 
CTCTCCTGCC TTCTCTATGG CCACACTGTC ACTGTGGTGG GGGGCCTGGG GGTGGCTGTG 
GTCTTTGCAG CCCTCCTGCT CAGAGTCTAT GCCCGGGGCC GTCTAAAGCA ACGGGGAAAG 
AAGGCTGTGC CTGTGGAGTC CCCTGTGCAA AAAGTTTGA