AARS2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	AARS2
Entrez Gene ID	474920
Full Name	alanyl-tRNA synthetase 2, mitochondrial
General protein information	Preferred Names alanyl-tRNA synthetase 2, mitochondrial Names alanine--tRNA ligase, mitochondrial alanyl-tRNA synthetase 2, mitochondrial (putative)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_532155.4	XP_532155.2	alanine--tRNA ligase, mitochondrial
XM_532155.5	XP_532155.2	alanine--tRNA ligase, mitochondrial

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AARS2	XP_518510.2
Mus musculus (house mouse)	Aars2	NP_941010.2
Homo sapiens (human)	AARS2	NP_065796.1
Rattus norvegicus (Norway rat)	Aars2	NP_001100361.1
Gallus gallus (chicken)	AARS2	NP_001026227.1
	AARS2	XP_001099647.1
Canis lupus familiaris (dog)	AARS2	XP_532155.2
Danio rerio (zebrafish)	aars2	XP_001332388.5
Bos taurus (cattle)	AARS2	NP_001178140.1

Gene Ontology

The following AARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe15335	XM_532155.5 Latest version!	Canis lupus familiaris alanyl-tRNA synthetase 2, mitochondrial (AARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OCe15335	XM_532155.4	Canis lupus familiaris alanyl-tRNA synthetase 2, mitochondrial (AARS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AARS2 ( XM_532155.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_532155.2 Canis lupus familiaris alanyl-tRNA synthetase 2, mitochondrial (AARS2), mRNA.

Price & Availability↑

CloneID	OCe15335
Clone ID Related Accession (Same CDS sequence)	XM_532155.4 , XM_532155.5
Accession Version	XM_532155.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	alanine--tRNA ligase, mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726081.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_532155.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT CGATGGCAGC GGCCGCCGGG GGACTGCGGC GGGCCATTCG AAGGTCGCCC 
CTGTGGGGGG GCCCCAGCCG CCGGCCGCTC TCATCCGAGC CCCCTCCGGC CCAGGCCACC 
GCCGTGCGAG ACGCCTTCCT AAGCTTCTTT CGGGACCGCC ATGGCCACCG GCTGGTGCCG 
TCCGCTTCCG TGCGGCCCCG CGGCGACCCC AGCTTGCTCT TCGTCAACGC CGGCATGAAC 
CAGTTCAAGC CGATCTTCCT GGGCACTGTG AATCCACGAA GCGAGATGGC AGGCTTCCGA 
CGTGTGGCCA ACAGCCAGAA ATGTGTGAGG GCTGGAGGAC GCCACAACGA CCTGGAAGAC 
GTGGGCCGGG ACCTTTCCCA TCATACCTTC TTCGAGATGC TCGGCAATTG GGCTTTTGGG 
GGTGAATATT TTAAGGAAGA GGCTTGCAGC ATGGCCTGGG AACTGCTGAC TCAAGTCTAT 
GGGATCCCTA AGGACAGGCT CTGGGTCTCC TACTTTGGTG GTGACCCCAA GGCAGGGTTG 
GACCCAGACC TAGAGAGCAG GGACATCTGG CTCAACTTAG GGGTGCATGC CAGCCATGTG 
CTTTCCTTTG GACCAGAAGA GAACTTCTGG GAGATGGGGG ACACTGGCCC ATGTGGGCCC 
TGTACCGAGA TCCACTATGA CCTGGCTGGT AGAGTGGGAG CCCCCCAGCT GGTGGAGCTT 
TGGAATCTGG TCTTCATTCA ACACAACAGA GAGACAGATG GAAGCCTGCA GCCCCTGCCC 
CAGCGGCACG TGGACACAGG AATGGGCCTG GAAAGACTGG TAGCTGTGCT GCAAGGCACA 
CCCTCCACCT ACGACACTGA CCTCTTTTCC CCGCTGCTCA AAGCCATACA CCAGGGTTGC 
GGGGCACCCC CTTACTTGGG CCAGGTCGGA GCAGCAGACA AGGGTCGCAC AGACATGGCA 
TATCGCGTGG TGGCCGATCA CATCCGCACA CTCAGTGTCT GCATCGCTGA TGGTGTCTAC 
CCTGGAATGT CGGGCGCCCC GCTGGTTCTT CGTCGGATCC TCCGTCGAGC TGTGCGGTTC 
TCTACGGAGG TGTTGCAGGC ACCACCGGGG TTCCTAGGCA GCCTGGTGCC TGTAGTGGTG 
GAGATACTGG GGGATACTTA TCCAGAACTA CAGAAGCACT CAGCCCAGAT AGCCAGCCTG 
GTATCAGAGG AGGAGGCAGC CTTCCTGGCC TCCCTGCAGC GGGGTCGGCG GATCATCGAT 
CAGACCCTGA GGTGCCTGGG GCCTTCTGAA TTGTTCCCTG CTGAAGTGGC CTGGTCCTTA 
TCACTGTCTG GGAACCTGGG GCTCCCCCTG GACCTGGTAG AGCTAATGCT GGAGGAGAAA 
GGGGTGCAGC TGGACTCTGC TGGGCTGGCA CGGTTGGCCC AGGAGGAGGC CAAGCTCCGG 
TCACGGCAGG CCGAGCCAGT TCAGAGGCAG GGACTGCAGC TGAATGTCCA CGCACTGGGG 
GAGCTGCAGC GCCGAGGCGT GCCCCCAACT GACGACAGTC CCAAGTACAA CTACTCTCTG 
CGGCCCAGCG GAGGCTATGA GTTCAACCCC TGTGAGGCCC AGGTGCTCCA GCTATATTTG 
GAGGATGGGA CAGCTGTGGC TTCTGTGGGG AAAGGCCAGC ACTGTGGCCT CCTCTTGGAC 
AAAACCAACT TCTACGCTGA ACAGGGGGGT CAGGCTTCAG ACCGAGGCTA CCTGGTGCGG 
GTGGGCCAGG AGGATGTGCT GTTCCCAGTG GCCCGGGCCC AGATCTGTGG AGGCTTCATC 
CTACACGAGG CTGTGGCCCC TGAGGGCCTG AAGGTGGGGG ACCAGGTGCA GCTACATGTG 
GATGAGGCCT GGCGTCTAGG CTGCATGGAG AAGCACACGG CCACGCACCT GCTAAACTGG 
GCCCTGCGGC AGGCCCTGGG CCCAGGCACG GAGCAGCGAG GTTCTCATCT CAATCCCGAG 
CGGCTGCGCT TTGACGTGGC CACCCAGGCT CCACTGACCC CAGAGCAGCT CCAGGCAGTG 
GAGGGCACTG TACAAGAGGC CGTGGGGCAG GATGAGGCCG TTTACATGGA GGAGGTGGCC 
CTGGCACACA CTGCTCATGT CCCTGGCCTG CGCTCTCTGG ATGAGGTATA CCCAGACCCT 
GTACGGGTGG TATCAGTGGG GGTGCCTGTG GCCCGTGCAC TGGACCCAGC CTCCCAAGCT 
GCACTGCAGA CCTCTGTGGA GCTGTGCTGC GGGACGCACC TGCTGCGCAC AGGGGCTGTA 
GGGGACCTCG TCATCATTGG GGAGCGCCAG CTCACCAAGG GCACTGTCCG CCTACTTGCT 
GTCACTGGGG AGCAGGCCCA GCAGGCCCGA GAGGTGGGCC AGAGCCTGGC CCAGGAAGTA 
GAAGCTGCCA CAGAGCGGCT TAGTCGAGGC AGCCAGGATG TAGTGGAGGC TCACCGACTA 
TCCAAGGACA TGGGACGACT CATTGATGCT GTGGACACGG CTGTGATGCC TCAGTGGCAG 
CGGCGGGAAC TGCAGGCTGC CCTAAAGGTG CTACAGCGGC GTGCCAACAC GGCCCTCCGA 
AAGCTGGAGC TGAGGCAGGC TGCACAGAAA ACCCAGGAGC TGCTGCAGCG GCACTCGGAG 
GGGCCCCTGA TCGTGGACAC AGTTGCCGCC GAGTCCCTCT CAGTGCTGGT GAAGGTGGTG 
CGGCAGCTGT GTGAGCGGGC ACCCTGCACA TCTGTGCTCC TCCTCAGCCC CCAGCCACAG 
GGGCACGTGC TGTGTGCCTG TCAGGTGGCT CAGAGTGCCA TGCCAGCCTT CACAGCAGAG 
GCCTGGGCAC TGGCGGTATG CAGCCACATG GGGGGCAAGG CCTGGGGCTC TCAAGTGGTG 
GCCCAGGGCA CCGGAACCAC TGCTGACCTA GAAGCTGCCC TCAGTACAGC TCGAACCTAT 
GCCCTCGACC AACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532155.2
CDS	221..3178
Translation	MAASMAAAAGGLRRAIRRSPLWGGPSRRPLSSEPPPAQATAVRDAFLSFFRDRHGHRLVPSASVRPRGDPSLLFVNAGMNQFKPIFLGTVNPRSEMAGFRRVANSQKCVRAGGRHNDLEDVGRDLSHHTFFEMLGNWAFGGEYFKEEACSMAWELLTQVYGIPKDRLWVSYFGGDPKAGLDPDLESRDIWLNLGVHASHVLSFGPEENFWEMGDTGPCGPCTEIHYDLAGRVGAPQLVELWNLVFIQHNRETDGSLQPLPQRHVDTGMGLERLVAVLQGTPSTYDTDLFSPLLKAIHQGCGAPPYLGQVGAADKGRTDMAYRVVADHIRTLSVCIADGVYPGMSGAPLVLRRILRRAVRFSTEVLQAPPGFLGSLVPVVVEILGDTYPELQKHSAQIASLVSEEEAAFLASLQRGRRIIDQTLRCLGPSELFPAEVAWSLSLSGNLGLPLDLVELMLEEKGVQLDSAGLARLAQEEAKLRSRQAEPVQRQGLQLNVHALGELQRRGVPPTDDSPKYNYSLRPSGGYEFNPCEAQVLQLYLEDGTAVASVGKGQHCGLLLDKTNFYAEQGGQASDRGYLVRVGQEDVLFPVARAQICGGFILHEAVAPEGLKVGDQVQLHVDEAWRLGCMEKHTATHLLNWALRQALGPGTEQRGSHLNPERLRFDVATQAPLTPEQLQAVEGTVQEAVGQDEAVYMEEVALAHTAHVPGLRSLDEVYPDPVRVVSVGVPVARALDPASQAALQTSVELCCGTHLLRTGAVGDLVIIGERQLTKGTVRLLAVTGEQAQQAREVGQSLAQEVEAATERLSRGSQDVVEAHRLSKDMGRLIDAVDTAVMPQWQRRELQAALKVLQRRANTALRKLELRQAAQKTQELLQRHSEGPLIVDTVAAESLSVLVKVVRQLCERAPCTSVLLLSPQPQGHVLCACQVAQSAMPAFTAEAWALAVCSHMGGKAWGSQVVAQGTGTTADLEAALSTARTYALDQL

Target ORF information:

RefSeq Version	XM_532155.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris alanyl-tRNA synthetase 2, mitochondrial (AARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532155.4

ORF Insert Sequence:

ATGGCGGCGT CGATGGCAGC GGCCGCCGGG GGACTGCGGC GGGCCATTCG AAGGTCGCCC 
CTGTGGGGGG GCCCCAGCCG CCGGCCGCTC TCATCCGAGC CCCCTCCGGC CCAGGCCACC 
GCCGTGCGAG ACGCCTTCCT AAGCTTCTTT CGGGACCGCC ATGGCCACCG GCTGGTGCCG 
TCCGCTTCCG TGCGGCCCCG CGGCGACCCC AGCTTGCTCT TCGTCAACGC CGGCATGAAC 
CAGTTCAAGC CGATCTTCCT GGGCACTGTG AATCCACGAA GCGAGATGGC AGGCTTCCGA 
CGTGTGGCCA ACAGCCAGAA ATGTGTGAGG GCTGGAGGAC GCCACAACGA CCTGGAAGAC 
GTGGGCCGGG ACCTTTCCCA TCATACCTTC TTCGAGATGC TCGGCAATTG GGCTTTTGGG 
GGTGAATATT TTAAGGAAGA GGCTTGCAGC ATGGCCTGGG AACTGCTGAC TCAAGTCTAT 
GGGATCCCTA AGGACAGGCT CTGGGTCTCC TACTTTGGTG GTGACCCCAA GGCAGGGTTG 
GACCCAGACC TAGAGAGCAG GGACATCTGG CTCAACTTAG GGGTGCATGC CAGCCATGTG 
CTTTCCTTTG GACCAGAAGA GAACTTCTGG GAGATGGGGG ACACTGGCCC ATGTGGGCCC 
TGTACCGAGA TCCACTATGA CCTGGCTGGT AGAGTGGGAG CCCCCCAGCT GGTGGAGCTT 
TGGAATCTGG TCTTCATTCA ACACAACAGA GAGACAGATG GAAGCCTGCA GCCCCTGCCC 
CAGCGGCACG TGGACACAGG AATGGGCCTG GAAAGACTGG TAGCTGTGCT GCAAGGCACA 
CCCTCCACCT ACGACACTGA CCTCTTTTCC CCGCTGCTCA AAGCCATACA CCAGGGTTGC 
GGGGCACCCC CTTACTTGGG CCAGGTCGGA GCAGCAGACA AGGGTCGCAC AGACATGGCA 
TATCGCGTGG TGGCCGATCA CATCCGCACA CTCAGTGTCT GCATCGCTGA TGGTGTCTAC 
CCTGGAATGT CGGGCGCCCC GCTGGTTCTT CGTCGGATCC TCCGTCGAGC TGTGCGGTTC 
TCTACGGAGG TGTTGCAGGC ACCACCGGGG TTCCTAGGCA GCCTGGTGCC TGTAGTGGTG 
GAGATACTGG GGGATACTTA TCCAGAACTA CAGAAGCACT CAGCCCAGAT AGCCAGCCTG 
GTATCAGAGG AGGAGGCAGC CTTCCTGGCC TCCCTGCAGC GGGGTCGGCG GATCATCGAT 
CAGACCCTGA GGTGCCTGGG GCCTTCTGAA TTGTTCCCTG CTGAAGTGGC CTGGTCCTTA 
TCACTGTCTG GGAACCTGGG GCTCCCCCTG GACCTGGTAG AGCTAATGCT GGAGGAGAAA 
GGGGTGCAGC TGGACTCTGC TGGGCTGGCA CGGTTGGCCC AGGAGGAGGC CAAGCTCCGG 
TCACGGCAGG CCGAGCCAGT TCAGAGGCAG GGACTGCAGC TGAATGTCCA CGCACTGGGG 
GAGCTGCAGC GCCGAGGCGT GCCCCCAACT GACGACAGTC CCAAGTACAA CTACTCTCTG 
CGGCCCAGCG GAGGCTATGA GTTCAACCCC TGTGAGGCCC AGGTGCTCCA GCTATATTTG 
GAGGATGGGA CAGCTGTGGC TTCTGTGGGG AAAGGCCAGC ACTGTGGCCT CCTCTTGGAC 
AAAACCAACT TCTACGCTGA ACAGGGGGGT CAGGCTTCAG ACCGAGGCTA CCTGGTGCGG 
GTGGGCCAGG AGGATGTGCT GTTCCCAGTG GCCCGGGCCC AGATCTGTGG AGGCTTCATC 
CTACACGAGG CTGTGGCCCC TGAGGGCCTG AAGGTGGGGG ACCAGGTGCA GCTACATGTG 
GATGAGGCCT GGCGTCTAGG CTGCATGGAG AAGCACACGG CCACGCACCT GCTAAACTGG 
GCCCTGCGGC AGGCCCTGGG CCCAGGCACG GAGCAGCGAG GTTCTCATCT CAATCCCGAG 
CGGCTGCGCT TTGACGTGGC CACCCAGGCT CCACTGACCC CAGAGCAGCT CCAGGCAGTG 
GAGGGCACTG TACAAGAGGC CGTGGGGCAG GATGAGGCCG TTTACATGGA GGAGGTGGCC 
CTGGCACACA CTGCTCATGT CCCTGGCCTG CGCTCTCTGG ATGAGGTATA CCCAGACCCT 
GTACGGGTGG TATCAGTGGG GGTGCCTGTG GCCCGTGCAC TGGACCCAGC CTCCCAAGCT 
GCACTGCAGA CCTCTGTGGA GCTGTGCTGC GGGACGCACC TGCTGCGCAC AGGGGCTGTA 
GGGGACCTCG TCATCATTGG GGAGCGCCAG CTCACCAAGG GCACTGTCCG CCTACTTGCT 
GTCACTGGGG AGCAGGCCCA GCAGGCCCGA GAGGTGGGCC AGAGCCTGGC CCAGGAAGTA 
GAAGCTGCCA CAGAGCGGCT TAGTCGAGGC AGCCAGGATG TAGTGGAGGC TCACCGACTA 
TCCAAGGACA TGGGACGACT CATTGATGCT GTGGACACGG CTGTGATGCC TCAGTGGCAG 
CGGCGGGAAC TGCAGGCTGC CCTAAAGGTG CTACAGCGGC GTGCCAACAC GGCCCTCCGA 
AAGCTGGAGC TGAGGCAGGC TGCACAGAAA ACCCAGGAGC TGCTGCAGCG GCACTCGGAG 
GGGCCCCTGA TCGTGGACAC AGTTGCCGCC GAGTCCCTCT CAGTGCTGGT GAAGGTGGTG 
CGGCAGCTGT GTGAGCGGGC ACCCTGCACA TCTGTGCTCC TCCTCAGCCC CCAGCCACAG 
GGGCACGTGC TGTGTGCCTG TCAGGTGGCT CAGAGTGCCA TGCCAGCCTT CACAGCAGAG 
GCCTGGGCAC TGGCGGTATG CAGCCACATG GGGGGCAAGG CCTGGGGCTC TCAAGTGGTG 
GCCCAGGGCA CCGGAACCAC TGCTGACCTA GAAGCTGCCC TCAGTACAGC TCGAACCTAT 
GCCCTCGACC AACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AARS2 ( XM_532155.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_532155.2 Canis lupus familiaris alanyl-tRNA synthetase 2, mitochondrial (AARS2), mRNA.

Price & Availability↑

CloneID	OCe15335
Clone ID Related Accession (Same CDS sequence)	XM_532155.4 , XM_532155.5
Accession Version	XM_532155.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	alanine--tRNA ligase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006594.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_532155.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGT CGATGGCAGC GGCCGCCGGG GGACTGCGGC GGGCCATTCG AAGGTCGCCC 
CTGTGGGGGG GCCCCAGCCG CCGGCCGCTC TCATCCGAGC CCCCTCCGGC CCAGGCCACC 
GCCGTGCGAG ACGCCTTCCT AAGCTTCTTT CGGGACCGCC ATGGCCACCG GCTGGTGCCG 
TCCGCTTCCG TGCGGCCCCG CGGCGACCCC AGCTTGCTCT TCGTCAACGC CGGCATGAAC 
CAGTTCAAGC CGATCTTCCT GGGCACTGTG AATCCACGAA GCGAGATGGC AGGCTTCCGA 
CGTGTGGCCA ACAGCCAGAA ATGTGTGAGG GCTGGAGGAC GCCACAACGA CCTGGAAGAC 
GTGGGCCGGG ACCTTTCCCA TCATACCTTC TTCGAGATGC TCGGCAATTG GGCTTTTGGG 
GGTGAATATT TTAAGGAAGA GGCTTGCAGC ATGGCCTGGG AACTGCTGAC TCAAGTCTAT 
GGGATCCCTA AGGACAGGCT CTGGGTCTCC TACTTTGGTG GTGACCCCAA GGCAGGGTTG 
GACCCAGACC TAGAGAGCAG GGACATCTGG CTCAACTTAG GGGTGCATGC CAGCCATGTG 
CTTTCCTTTG GACCAGAAGA GAACTTCTGG GAGATGGGGG ACACTGGCCC ATGTGGGCCC 
TGTACCGAGA TCCACTATGA CCTGGCTGGT AGAGTGGGAG CCCCCCAGCT GGTGGAGCTT 
TGGAATCTGG TCTTCATTCA ACACAACAGA GAGACAGATG GAAGCCTGCA GCCCCTGCCC 
CAGCGGCACG TGGACACAGG AATGGGCCTG GAAAGACTGG TAGCTGTGCT GCAAGGCACA 
CCCTCCACCT ACGACACTGA CCTCTTTTCC CCGCTGCTCA AAGCCATACA CCAGGGTTGC 
GGGGCACCCC CTTACTTGGG CCAGGTCGGA GCAGCAGACA AGGGTCGCAC AGACATGGCA 
TATCGCGTGG TGGCCGATCA CATCCGCACA CTCAGTGTCT GCATCGCTGA TGGTGTCTAC 
CCTGGAATGT CGGGCGCCCC GCTGGTTCTT CGTCGGATCC TCCGTCGAGC TGTGCGGTTC 
TCTACGGAGG TGTTGCAGGC ACCACCGGGG TTCCTAGGCA GCCTGGTGCC TGTAGTGGTG 
GAGATACTGG GGGATACTTA TCCAGAACTA CAGAAGCACT CAGCCCAGAT AGCCAGCCTG 
GTATCAGAGG AGGAGGCAGC CTTCCTGGCC TCCCTGCAGC GGGGTCGGCG GATCATCGAT 
CAGACCCTGA GGTGCCTGGG GCCTTCTGAA TTGTTCCCTG CTGAAGTGGC CTGGTCCTTA 
TCACTGTCTG GGAACCTGGG GCTCCCCCTG GACCTGGTAG AGCTAATGCT GGAGGAGAAA 
GGGGTGCAGC TGGACTCTGC TGGGCTGGCA CGGTTGGCCC AGGAGGAGGC CAAGCTCCGG 
TCACGGCAGG CCGAGCCAGT TCAGAGGCAG GGACTGCAGC TGAATGTCCA CGCACTGGGG 
GAGCTGCAGC GCCGAGGCGT GCCCCCAACT GACGACAGTC CCAAGTACAA CTACTCTCTG 
CGGCCCAGCG GAGGCTATGA GTTCAACCCC TGTGAGGCCC AGGTGCTCCA GCTATATTTG 
GAGGATGGGA CAGCTGTGGC TTCTGTGGGG AAAGGCCAGC ACTGTGGCCT CCTCTTGGAC 
AAAACCAACT TCTACGCTGA ACAGGGGGGT CAGGCTTCAG ACCGAGGCTA CCTGGTGCGG 
GTGGGCCAGG AGGATGTGCT GTTCCCAGTG GCCCGGGCCC AGATCTGTGG AGGCTTCATC 
CTACACGAGG CTGTGGCCCC TGAGGGCCTG AAGGTGGGGG ACCAGGTGCA GCTACATGTG 
GATGAGGCCT GGCGTCTAGG CTGCATGGAG AAGCACACGG CCACGCACCT GCTAAACTGG 
GCCCTGCGGC AGGCCCTGGG CCCAGGCACG GAGCAGCGAG GTTCTCATCT CAATCCCGAG 
CGGCTGCGCT TTGACGTGGC CACCCAGGCT CCACTGACCC CAGAGCAGCT CCAGGCAGTG 
GAGGGCACTG TACAAGAGGC CGTGGGGCAG GATGAGGCCG TTTACATGGA GGAGGTGGCC 
CTGGCACACA CTGCTCATGT CCCTGGCCTG CGCTCTCTGG ATGAGGTATA CCCAGACCCT 
GTACGGGTGG TATCAGTGGG GGTGCCTGTG GCCCGTGCAC TGGACCCAGC CTCCCAAGCT 
GCACTGCAGA CCTCTGTGGA GCTGTGCTGC GGGACGCACC TGCTGCGCAC AGGGGCTGTA 
GGGGACCTCG TCATCATTGG GGAGCGCCAG CTCACCAAGG GCACTGTCCG CCTACTTGCT 
GTCACTGGGG AGCAGGCCCA GCAGGCCCGA GAGGTGGGCC AGAGCCTGGC CCAGGAAGTA 
GAAGCTGCCA CAGAGCGGCT TAGTCGAGGC AGCCAGGATG TAGTGGAGGC TCACCGACTA 
TCCAAGGACA TGGGACGACT CATTGATGCT GTGGACACGG CTGTGATGCC TCAGTGGCAG 
CGGCGGGAAC TGCAGGCTGC CCTAAAGGTG CTACAGCGGC GTGCCAACAC GGCCCTCCGA 
AAGCTGGAGC TGAGGCAGGC TGCACAGAAA ACCCAGGAGC TGCTGCAGCG GCACTCGGAG 
GGGCCCCTGA TCGTGGACAC AGTTGCCGCC GAGTCCCTCT CAGTGCTGGT GAAGGTGGTG 
CGGCAGCTGT GTGAGCGGGC ACCCTGCACA TCTGTGCTCC TCCTCAGCCC CCAGCCACAG 
GGGCACGTGC TGTGTGCCTG TCAGGTGGCT CAGAGTGCCA TGCCAGCCTT CACAGCAGAG 
GCCTGGGCAC TGGCGGTATG CAGCCACATG GGGGGCAAGG CCTGGGGCTC TCAAGTGGTG 
GCCCAGGGCA CCGGAACCAC TGCTGACCTA GAAGCTGCCC TCAGTACAGC TCGAACCTAT 
GCCCTCGACC AACTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_532155.2
CDS	1..2958
Translation	MAASMAAAAGGLRRAIRRSPLWGGPSRRPLSSEPPPAQATAVRDAFLSFFRDRHGHRLVPSASVRPRGDPSLLFVNAGMNQFKPIFLGTVNPRSEMAGFRRVANSQKCVRAGGRHNDLEDVGRDLSHHTFFEMLGNWAFGGEYFKEEACSMAWELLTQVYGIPKDRLWVSYFGGDPKAGLDPDLESRDIWLNLGVHASHVLSFGPEENFWEMGDTGPCGPCTEIHYDLAGRVGAPQLVELWNLVFIQHNRETDGSLQPLPQRHVDTGMGLERLVAVLQGTPSTYDTDLFSPLLKAIHQGCGAPPYLGQVGAADKGRTDMAYRVVADHIRTLSVCIADGVYPGMSGAPLVLRRILRRAVRFSTEVLQAPPGFLGSLVPVVVEILGDTYPELQKHSAQIASLVSEEEAAFLASLQRGRRIIDQTLRCLGPSELFPAEVAWSLSLSGNLGLPLDLVELMLEEKGVQLDSAGLARLAQEEAKLRSRQAEPVQRQGLQLNVHALGELQRRGVPPTDDSPKYNYSLRPSGGYEFNPCEAQVLQLYLEDGTAVASVGKGQHCGLLLDKTNFYAEQGGQASDRGYLVRVGQEDVLFPVARAQICGGFILHEAVAPEGLKVGDQVQLHVDEAWRLGCMEKHTATHLLNWALRQALGPGTEQRGSHLNPERLRFDVATQAPLTPEQLQAVEGTVQEAVGQDEAVYMEEVALAHTAHVPGLRSLDEVYPDPVRVVSVGVPVARALDPASQAALQTSVELCCGTHLLRTGAVGDLVIIGERQLTKGTVRLLAVTGEQAQQAREVGQSLAQEVEAATERLSRGSQDVVEAHRLSKDMGRLIDAVDTAVMPQWQRRELQAALKVLQRRANTALRKLELRQAAQKTQELLQRHSEGPLIVDTVAAESLSVLVKVVRQLCERAPCTSVLLLSPQPQGHVLCACQVAQSAMPAFTAEAWALAVCSHMGGKAWGSQVVAQGTGTTADLEAALSTARTYALDQL

Target ORF information:

RefSeq Version	XM_532155.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris alanyl-tRNA synthetase 2, mitochondrial (AARS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_532155.5

ORF Insert Sequence:

ATGGCGGCGT CGATGGCAGC GGCCGCCGGG GGACTGCGGC GGGCCATTCG AAGGTCGCCC 
CTGTGGGGGG GCCCCAGCCG CCGGCCGCTC TCATCCGAGC CCCCTCCGGC CCAGGCCACC 
GCCGTGCGAG ACGCCTTCCT AAGCTTCTTT CGGGACCGCC ATGGCCACCG GCTGGTGCCG 
TCCGCTTCCG TGCGGCCCCG CGGCGACCCC AGCTTGCTCT TCGTCAACGC CGGCATGAAC 
CAGTTCAAGC CGATCTTCCT GGGCACTGTG AATCCACGAA GCGAGATGGC AGGCTTCCGA 
CGTGTGGCCA ACAGCCAGAA ATGTGTGAGG GCTGGAGGAC GCCACAACGA CCTGGAAGAC 
GTGGGCCGGG ACCTTTCCCA TCATACCTTC TTCGAGATGC TCGGCAATTG GGCTTTTGGG 
GGTGAATATT TTAAGGAAGA GGCTTGCAGC ATGGCCTGGG AACTGCTGAC TCAAGTCTAT 
GGGATCCCTA AGGACAGGCT CTGGGTCTCC TACTTTGGTG GTGACCCCAA GGCAGGGTTG 
GACCCAGACC TAGAGAGCAG GGACATCTGG CTCAACTTAG GGGTGCATGC CAGCCATGTG 
CTTTCCTTTG GACCAGAAGA GAACTTCTGG GAGATGGGGG ACACTGGCCC ATGTGGGCCC 
TGTACCGAGA TCCACTATGA CCTGGCTGGT AGAGTGGGAG CCCCCCAGCT GGTGGAGCTT 
TGGAATCTGG TCTTCATTCA ACACAACAGA GAGACAGATG GAAGCCTGCA GCCCCTGCCC 
CAGCGGCACG TGGACACAGG AATGGGCCTG GAAAGACTGG TAGCTGTGCT GCAAGGCACA 
CCCTCCACCT ACGACACTGA CCTCTTTTCC CCGCTGCTCA AAGCCATACA CCAGGGTTGC 
GGGGCACCCC CTTACTTGGG CCAGGTCGGA GCAGCAGACA AGGGTCGCAC AGACATGGCA 
TATCGCGTGG TGGCCGATCA CATCCGCACA CTCAGTGTCT GCATCGCTGA TGGTGTCTAC 
CCTGGAATGT CGGGCGCCCC GCTGGTTCTT CGTCGGATCC TCCGTCGAGC TGTGCGGTTC 
TCTACGGAGG TGTTGCAGGC ACCACCGGGG TTCCTAGGCA GCCTGGTGCC TGTAGTGGTG 
GAGATACTGG GGGATACTTA TCCAGAACTA CAGAAGCACT CAGCCCAGAT AGCCAGCCTG 
GTATCAGAGG AGGAGGCAGC CTTCCTGGCC TCCCTGCAGC GGGGTCGGCG GATCATCGAT 
CAGACCCTGA GGTGCCTGGG GCCTTCTGAA TTGTTCCCTG CTGAAGTGGC CTGGTCCTTA 
TCACTGTCTG GGAACCTGGG GCTCCCCCTG GACCTGGTAG AGCTAATGCT GGAGGAGAAA 
GGGGTGCAGC TGGACTCTGC TGGGCTGGCA CGGTTGGCCC AGGAGGAGGC CAAGCTCCGG 
TCACGGCAGG CCGAGCCAGT TCAGAGGCAG GGACTGCAGC TGAATGTCCA CGCACTGGGG 
GAGCTGCAGC GCCGAGGCGT GCCCCCAACT GACGACAGTC CCAAGTACAA CTACTCTCTG 
CGGCCCAGCG GAGGCTATGA GTTCAACCCC TGTGAGGCCC AGGTGCTCCA GCTATATTTG 
GAGGATGGGA CAGCTGTGGC TTCTGTGGGG AAAGGCCAGC ACTGTGGCCT CCTCTTGGAC 
AAAACCAACT TCTACGCTGA ACAGGGGGGT CAGGCTTCAG ACCGAGGCTA CCTGGTGCGG 
GTGGGCCAGG AGGATGTGCT GTTCCCAGTG GCCCGGGCCC AGATCTGTGG AGGCTTCATC 
CTACACGAGG CTGTGGCCCC TGAGGGCCTG AAGGTGGGGG ACCAGGTGCA GCTACATGTG 
GATGAGGCCT GGCGTCTAGG CTGCATGGAG AAGCACACGG CCACGCACCT GCTAAACTGG 
GCCCTGCGGC AGGCCCTGGG CCCAGGCACG GAGCAGCGAG GTTCTCATCT CAATCCCGAG 
CGGCTGCGCT TTGACGTGGC CACCCAGGCT CCACTGACCC CAGAGCAGCT CCAGGCAGTG 
GAGGGCACTG TACAAGAGGC CGTGGGGCAG GATGAGGCCG TTTACATGGA GGAGGTGGCC 
CTGGCACACA CTGCTCATGT CCCTGGCCTG CGCTCTCTGG ATGAGGTATA CCCAGACCCT 
GTACGGGTGG TATCAGTGGG GGTGCCTGTG GCCCGTGCAC TGGACCCAGC CTCCCAAGCT 
GCACTGCAGA CCTCTGTGGA GCTGTGCTGC GGGACGCACC TGCTGCGCAC AGGGGCTGTA 
GGGGACCTCG TCATCATTGG GGAGCGCCAG CTCACCAAGG GCACTGTCCG CCTACTTGCT 
GTCACTGGGG AGCAGGCCCA GCAGGCCCGA GAGGTGGGCC AGAGCCTGGC CCAGGAAGTA 
GAAGCTGCCA CAGAGCGGCT TAGTCGAGGC AGCCAGGATG TAGTGGAGGC TCACCGACTA 
TCCAAGGACA TGGGACGACT CATTGATGCT GTGGACACGG CTGTGATGCC TCAGTGGCAG 
CGGCGGGAAC TGCAGGCTGC CCTAAAGGTG CTACAGCGGC GTGCCAACAC GGCCCTCCGA 
AAGCTGGAGC TGAGGCAGGC TGCACAGAAA ACCCAGGAGC TGCTGCAGCG GCACTCGGAG 
GGGCCCCTGA TCGTGGACAC AGTTGCCGCC GAGTCCCTCT CAGTGCTGGT GAAGGTGGTG 
CGGCAGCTGT GTGAGCGGGC ACCCTGCACA TCTGTGCTCC TCCTCAGCCC CCAGCCACAG 
GGGCACGTGC TGTGTGCCTG TCAGGTGGCT CAGAGTGCCA TGCCAGCCTT CACAGCAGAG 
GCCTGGGCAC TGGCGGTATG CAGCCACATG GGGGGCAAGG CCTGGGGCTC TCAAGTGGTG 
GCCCAGGGCA CCGGAACCAC TGCTGACCTA GAAGCTGCCC TCAGTACAGC TCGAACCTAT 
GCCCTCGACC AACTCTGA