ZP2 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZP2
Entrez Gene ID	403988
Full Name	zona pellucida glycoprotein 2
Synonyms	ZPA,Zp-2
General protein information	Preferred Names zona pellucida glycoprotein 2 Names zona pellucida sperm-binding protein 2 zona pellucida A protein zona pellucida glycoprotein 2 (sperm receptor) zona pellucida glycoprotein ZP2 zona pellucida protein A
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003304.1	NP_001003304.1	zona pellucida sperm-binding protein 2 precursor

Pathways from BioSystems

Homologies

	ZP2	XP_001091147.2
Canis lupus familiaris (dog)	ZP2	NP_001003304.1
Gallus gallus (chicken)	ZP2	NP_001034187.1
Xenopus tropicalis (tropical clawed frog)	zp2	NP_988855.1
Pan troglodytes (chimpanzee)	ZP2	XP_510869.3
Mus musculus (house mouse)	Zp2	NP_035905.1
Homo sapiens (human)	ZP2	NP_003451.1
Bos taurus (cattle)	ZP2	NP_776398.1
Rattus norvegicus (Norway rat)	Zp2	NP_112412.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and after in vitro maturation.
Kempisty B, Piotrowska H, Bukowska D, Wo?na M, Ciesi??ka S, Wojtanowicz-Markiewicz K, W?odarczyk R, Jopek K, Jeseta M, Bruska M, Nowicki M, Ja?kowski JM, Br?ssow KP, Zabel M
Zygote (Cambridge, England)23(6)863-73(2015 Dec)

Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.
Burkart AD, Xiong B, Baibakov B, Jim?nez-Movilla M, Dean J
The Journal of cell biology197(1)37-44(2012 Apr)

Evaluation of the immunocontraceptive potential of Escherichia coli-expressed recombinant dog ZP2 and ZP3 in a homologous animal model.
Srivastava N, Santhanam R, Sheela P, Mukund S, Thakral SS, Malik BS, Gupta SK
Reproduction (Cambridge, England)123(6)847-57(2002 Jun)

Cloning and characterization of zona pellucida genes and cDNAs from a variety of mammalian species: the ZPA, ZPB and ZPC gene families.
Harris JD, Hibler DW, Fontenot GK, Hsu KT, Yurewicz EC, Sacco AG
DNA sequence : the journal of DNA sequencing and mapping4(6)361-93(1994)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of ZP2 and ZP3 genes is associated with the maturation stage of canine oocytes.
Title: Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and after in vitro maturation.

The following ZP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00086	NM_001003304.1 Latest version!	Canis lupus familiaris zona pellucida glycoprotein 2 (ZP2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZP2 ( NM_001003304.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003304.1 Canis lupus familiaris zona pellucida glycoprotein 2 (ZP2), mRNA.

Price & Availability↑

CloneID	OCe00086
Clone ID Related Accession (Same CDS sequence)	NM_001003304.1
Accession Version	NM_001003304.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Canis lupus familiaris(dog)
Product	zona pellucida sperm-binding protein 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U05779.1. ##Evidence-Data-START## Transcript exon combination :: U05779.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00013597 [ECO:0000348] ##Evidence-Data-END##

ATGGCATGCA AACAGAAAGG AGACAGTGGG AGTCCCTCAA GCAGGTTTAG TGCAGATTGG 
AGCACCTACA GGTCACTTTC TTTATTCTTC ATCCTTGTGA CTTCAGTGAA CTCAGTAGGT 
GTTATGCAGT TGGTGAATCC CATCTTCCCA GGTACTGTCA TTTGCCATGA AAATAAAATG 
ACAGTGGAAT TTCCAAGGGA TCTTGGCACC AAAAAATGGC ATGCATCTGT GGTGGATCCA 
TTTAGTTTTG AATTGTTGAA CTGTACTTCT ATCCTGGACC CAGAAAAGCT CACCCTGAAG 
GCCCCATATG AGACCTGTAG CAGGAGAGTG CTTGGCCAGC ATCAGATGGC CATCAGACTC 
ACGGACAACA ATGCTGCTTC AAGACATAAG GCTTTCATGT ATCAGATCAG CTGTCCAGTT 
ATGCAAACAG AAGAAACCCA TGAGCATGCA GGATCCACAA TCTGCACAAA AGATTCCATG 
TCTTTTACCT TTAACATTAT TCCTGGCATG GCTGATGAAA ATACGAATCC CAGTGGTGGG 
AAATGGATGA TGGAGGTTGA TGATGCAAAA GCTCAAAATC TGACTCTTCG GGAGGCCTTG 
ATGCAAGGAT ATAATTTCCT GTTTGATAGC CACAGGCTCA GTGTCCAAGT GTCATTCAAT 
GCCACTGGAG TCACTCACTA CATGCAAGGT AACAGTCACC TCTACACAGT GCCTCTGAAG 
CTTATACACA CATCTCCTGG GCAGAAGATC ATCTTAACAA CACGAGTACT TTGTATGTCA 
GATCCCGTGA CCTGTAACGC CACACACATG ACCCTCACCA TACCAGAGTT TCCTGGGAAA 
CTACAGTCTG TGAGATTTGA AAACACGAAC TTTCGTGTAA GCCAGCTGCA CAACCATGGG 
ATTGATAAAG AAGAATTAAA CGGCTTGAGG TTACACTTCA GCAAATCTCT TCTCAAAATG 
AACTCCTCTG AAAAATGCCT ACTCTATCAG TTCTACTTAG CATCTCTCAA GCTGACCTTT 
GCCTTTGAAC GGGACACGGT TTCCACAGTG GTTTATCCTG AGTGTGTTTG TGAGCCACCA 
GTTACTATAG TTACAGGTGA CCTGTGTACC CAGGATGGGT TTATGGATGT CAAGGTCTAC 
AGCCACCAAA CAAAACCAGC TCTAAACTTG GATACCCTCA GAGTGGGAGA CTCCTCCTGC 
CAACCTACTT TCAAGGCTCC ATCACAAGGG TTGACACTGT TTCACATCCC CCTAAATGGA 
TGTGGAACAA GACTTAAGTT CAAAGGTGAC ACAGTCATCT ATGAAAATGA AATACATGCT 
CTCTGGACAG ATCTCCCTCC AAGCACAATT TCCAGAGATA GTGAATTCAG AATGACTGTG 
AAGTGCCATT ACAGCAGAGA TGACCTGCTG ATAAATACCA ATGTCCAAAG TCTTCCTCCT 
CCCGTGGCCT CAGTGAGGCC TGGTCCACTT GCCTTAATCC TGCAAACCTA CCCAGATAAA 
TCCTATTTGC GACCCTATGG GGATAAGGAG TATCCTGTGG TGAGATACCT CCGCCAACCA 
ATTTACCTGG AAGTGAAAGT CCTAAATAGG GCTGACCCCA ACATCAAGCT GGTCTTAGAT 
GATTGCTGGG CAACACCCAC CATGGACCCA GCCTCACTCC CCCAGTGGAA TATTGTCATG 
GATGGCTGTG AATACAATCT GGACAACTAC AGAACGACCT TCCATCCAGT TGGCTCCTCT 
GTGACCTACC CTACTCACTA TCAGAGGTTT GATGTGAAGA CCTTTGCCTT TATATCAGAG 
GCCCAAGTGC TTTCTAGCCT GGTCTACTTC CACTGCACCG CATTAATCTG CAATCGACTG 
TCTCCTGACT CCCCTCTGTG TTCTGTGACT TGCCCTGTAT CATCCAGGCA CAGGCGAGCC 
ACAGGCAGTA CTGAAGAAGA GAAGATGATA GTAAGTCTCC CGGGACCCAT CCTCCTGTTG 
GCAGACAGCT CTTCACTCAG AGATGGTGTG GACTCAAAAG GGCACAGGGC TGCTGGATAT 
GTTGCTTTTA AAACTGTAGT GGCTGTGGCT GCCTTAGCAG GCCTTGTGGC TGCTCTAGGT 
CTCATCATCT ACCTGCGTAA GAAAAGAACC ATGGTGTTAA ATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003304.1
CDS	196..2343
Translation	MACKQKGDSGSPSSRFSADWSTYRSLSLFFILVTSVNSVGVMQLVNPIFPGTVICHENKMTVEFPRDLGTKKWHASVVDPFSFELLNCTSILDPEKLTLKAPYETCSRRVLGQHQMAIRLTDNNAASRHKAFMYQISCPVMQTEETHEHAGSTICTKDSMSFTFNIIPGMADENTNPSGGKWMMEVDDAKAQNLTLREALMQGYNFLFDSHRLSVQVSFNATGVTHYMQGNSHLYTVPLKLIHTSPGQKIILTTRVLCMSDPVTCNATHMTLTIPEFPGKLQSVRFENTNFRVSQLHNHGIDKEELNGLRLHFSKSLLKMNSSEKCLLYQFYLASLKLTFAFERDTVSTVVYPECVCEPPVTIVTGDLCTQDGFMDVKVYSHQTKPALNLDTLRVGDSSCQPTFKAPSQGLTLFHIPLNGCGTRLKFKGDTVIYENEIHALWTDLPPSTISRDSEFRMTVKCHYSRDDLLINTNVQSLPPPVASVRPGPLALILQTYPDKSYLRPYGDKEYPVVRYLRQPIYLEVKVLNRADPNIKLVLDDCWATPTMDPASLPQWNIVMDGCEYNLDNYRTTFHPVGSSVTYPTHYQRFDVKTFAFISEAQVLSSLVYFHCTALICNRLSPDSPLCSVTCPVSSRHRRATGSTEEEKMIVSLPGPILLLADSSSLRDGVDSKGHRAAGYVAFKTVVAVAALAGLVAALGLIIYLRKKRTMVLNH

Target ORF information:

RefSeq Version	NM_001003304.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris zona pellucida glycoprotein 2 (ZP2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003304.1

ORF Insert Sequence:

ATGGCATGCA AACAGAAAGG AGACAGTGGG AGTCCCTCAA GCAGGTTTAG TGCAGATTGG 
AGCACCTACA GGTCACTTTC TTTATTCTTC ATCCTTGTGA CTTCAGTGAA CTCAGTAGGT 
GTTATGCAGT TGGTGAATCC CATCTTCCCA GGTACTGTCA TTTGCCATGA AAATAAAATG 
ACAGTGGAAT TTCCAAGGGA TCTTGGCACC AAAAAATGGC ATGCATCTGT GGTGGATCCA 
TTTAGTTTTG AATTGTTGAA CTGTACTTCT ATCCTGGACC CAGAAAAGCT CACCCTGAAG 
GCCCCATATG AGACCTGTAG CAGGAGAGTG CTTGGCCAGC ATCAGATGGC CATCAGACTC 
ACGGACAACA ATGCTGCTTC AAGACATAAG GCTTTCATGT ATCAGATCAG CTGTCCAGTT 
ATGCAAACAG AAGAAACCCA TGAGCATGCA GGATCCACAA TCTGCACAAA AGATTCCATG 
TCTTTTACCT TTAACATTAT TCCTGGCATG GCTGATGAAA ATACGAATCC CAGTGGTGGG 
AAATGGATGA TGGAGGTTGA TGATGCAAAA GCTCAAAATC TGACTCTTCG GGAGGCCTTG 
ATGCAAGGAT ATAATTTCCT GTTTGATAGC CACAGGCTCA GTGTCCAAGT GTCATTCAAT 
GCCACTGGAG TCACTCACTA CATGCAAGGT AACAGTCACC TCTACACAGT GCCTCTGAAG 
CTTATACACA CATCTCCTGG GCAGAAGATC ATCTTAACAA CACGAGTACT TTGTATGTCA 
GATCCCGTGA CCTGTAACGC CACACACATG ACCCTCACCA TACCAGAGTT TCCTGGGAAA 
CTACAGTCTG TGAGATTTGA AAACACGAAC TTTCGTGTAA GCCAGCTGCA CAACCATGGG 
ATTGATAAAG AAGAATTAAA CGGCTTGAGG TTACACTTCA GCAAATCTCT TCTCAAAATG 
AACTCCTCTG AAAAATGCCT ACTCTATCAG TTCTACTTAG CATCTCTCAA GCTGACCTTT 
GCCTTTGAAC GGGACACGGT TTCCACAGTG GTTTATCCTG AGTGTGTTTG TGAGCCACCA 
GTTACTATAG TTACAGGTGA CCTGTGTACC CAGGATGGGT TTATGGATGT CAAGGTCTAC 
AGCCACCAAA CAAAACCAGC TCTAAACTTG GATACCCTCA GAGTGGGAGA CTCCTCCTGC 
CAACCTACTT TCAAGGCTCC ATCACAAGGG TTGACACTGT TTCACATCCC CCTAAATGGA 
TGTGGAACAA GACTTAAGTT CAAAGGTGAC ACAGTCATCT ATGAAAATGA AATACATGCT 
CTCTGGACAG ATCTCCCTCC AAGCACAATT TCCAGAGATA GTGAATTCAG AATGACTGTG 
AAGTGCCATT ACAGCAGAGA TGACCTGCTG ATAAATACCA ATGTCCAAAG TCTTCCTCCT 
CCCGTGGCCT CAGTGAGGCC TGGTCCACTT GCCTTAATCC TGCAAACCTA CCCAGATAAA 
TCCTATTTGC GACCCTATGG GGATAAGGAG TATCCTGTGG TGAGATACCT CCGCCAACCA 
ATTTACCTGG AAGTGAAAGT CCTAAATAGG GCTGACCCCA ACATCAAGCT GGTCTTAGAT 
GATTGCTGGG CAACACCCAC CATGGACCCA GCCTCACTCC CCCAGTGGAA TATTGTCATG 
GATGGCTGTG AATACAATCT GGACAACTAC AGAACGACCT TCCATCCAGT TGGCTCCTCT 
GTGACCTACC CTACTCACTA TCAGAGGTTT GATGTGAAGA CCTTTGCCTT TATATCAGAG 
GCCCAAGTGC TTTCTAGCCT GGTCTACTTC CACTGCACCG CATTAATCTG CAATCGACTG 
TCTCCTGACT CCCCTCTGTG TTCTGTGACT TGCCCTGTAT CATCCAGGCA CAGGCGAGCC 
ACAGGCAGTA CTGAAGAAGA GAAGATGATA GTAAGTCTCC CGGGACCCAT CCTCCTGTTG 
GCAGACAGCT CTTCACTCAG AGATGGTGTG GACTCAAAAG GGCACAGGGC TGCTGGATAT 
GTTGCTTTTA AAACTGTAGT GGCTGTGGCT GCCTTAGCAG GCCTTGTGGC TGCTCTAGGT 
CTCATCATCT ACCTGCGTAA GAAAAGAACC ATGGTGTTAA ATCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and cellular distribution of zona pellucida glycoproteins in canine oocytes before and after in vitro maturation.
Zygote (Cambridge, England)23(6)863-73(2015 Dec)
Kempisty B,Piotrowska H,Bukowska D,Wo?na M,Ciesi??ka S,Wojtanowicz-Markiewicz K,W?odarczyk R,Jopek K,Jeseta M,Bruska M,Nowicki M,Ja?kowski JM,Br?ssow KP,Zabel M

Ovastacin, a cortical granule protease, cleaves ZP2 in the zona pellucida to prevent polyspermy.
The Journal of cell biology197(1)37-44(2012 Apr)
Burkart AD,Xiong B,Baibakov B,Jim?nez-Movilla M,Dean J

Evaluation of the immunocontraceptive potential of Escherichia coli-expressed recombinant dog ZP2 and ZP3 in a homologous animal model.
Reproduction (Cambridge, England)123(6)847-57(2002 Jun)
Srivastava N,Santhanam R,Sheela P,Mukund S,Thakral SS,Malik BS,Gupta SK

Cloning and characterization of zona pellucida genes and cDNAs from a variety of mammalian species: the ZPA, ZPB and ZPC gene families.
DNA sequence : the journal of DNA sequencing and mapping4(6)361-93(1994)
Harris JD,Hibler DW,Fontenot GK,Hsu KT,Yurewicz EC,Sacco AG