TRDN cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRDN
Entrez Gene ID	403776
Full Name	triadin
General protein information	Preferred Names triadin Names triadin
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001003154.2	NP_001003154.2	triadin

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	TRDN	NP_001003154.2
Homo sapiens (human)	TRDN	NP_006064.2
Bos taurus (cattle)	TRDN	XP_003586508.2
Mus musculus (house mouse)	Trdn	NP_084002.2
Rattus norvegicus (Norway rat)	Trdn	NP_067698.1
	TRDN	XP_001107956.2

Gene Ontology
Bibliography

Related articles in PubMed

Inefficient glycosylation leads to high steady-state levels of actively degrading cardiac triadin-1.
Milstein ML, McFarland TP, Marsh JD, Cala SE
The Journal of biological chemistry283(4)1929-35(2008 Jan)

Triadin overexpression stimulates excitation-contraction coupling and increases predisposition to cellular arrhythmia in cardiac myocytes.
Terentyev D, Cala SE, Houle TD, Viatchenko-Karpinski S, Gyorke I, Terentyeva R, Williams SC, Gyorke S
Circulation research96(6)651-8(2005 Apr)

The role of calsequestrin, triadin, and junctin in conferring cardiac ryanodine receptor responsiveness to luminal calcium.
Gy?rke I, Hester N, Jones LR, Gy?rke S
Biophysical journal86(4)2121-8(2004 Apr)

Cardiac hypertrophy and impaired relaxation in transgenic mice overexpressing triadin 1.
Kirchhefer U, Neumann J, Baba HA, Begrow F, Kobayashi YM, Reinke U, Schmitz W, Jones LR
The Journal of biological chemistry276(6)4142-9(2001 Feb)

Identification of triadin 1 as the predominant triadin isoform expressed in mammalian myocardium.
Kobayashi YM, Jones LR
The Journal of biological chemistry274(40)28660-8(1999 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

proteasome inhibition led to an accumulation of two new modified forms of triadin-1 that were seen with triadin-1 only when it is not glycosylated on Asn(75).
Title: Inefficient glycosylation leads to high steady-state levels of actively degrading cardiac triadin-1.

TRD enhances cardiac excitation-contraction coupling by directly stimulating the RyR2. Interaction of TRD with RyR2 may involve amino acids 200 to 224 in C-terminal domain of TRD.
Title: Triadin overexpression stimulates excitation-contraction coupling and increases predisposition to cellular arrhythmia in cardiac myocytes.

The following TRDN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRDN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00251	NM_001003154.2 Latest version!	Canis lupus familiaris triadin (TRDN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRDN ( NM_001003154.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001003154.2 Canis lupus familiaris triadin (TRDN), mRNA.

Price & Availability↑

CloneID	OCe00251
Clone ID Related Accession (Same CDS sequence)	NM_001003154.2
Accession Version	NM_001003154.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Canis lupus familiaris(dog)
Product	triadin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF165916.1. On Jun 4, 2008 this sequence version replaced NM_001003154.1. ##Evidence-Data-START## Transcript exon combination :: AF165916.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2240594, SAMEA4547738 [ECO:0000350] ##Evidence-Data-END##

ATGACTGAGA TCACTGCTGA AGGAAATGCA TCTACTACCA CAACTGTGAT CGACAGCAAA 
AATGGATCTG TGCCCAAATC CCCTGGAAAA GTGCTGAAGA GGACAGTCAC AGAAGACATA 
GTGACAACAT TCAGCTCCCC AGCAGCCTGG CTTCTGGTCA TTGCTCTGAT CATCACATGG 
TCAGCTGTTG CCGTTGTTAT GTTTGACCTA GTGGATTACA AAAACTTTTC AGCGAGCTCT 
CTTTCCAAGA TTGGCTCAGA TCCTCTAAAA CTTGTACATG ATGCTGTGGA AGAAACCACG 
GACTGGGTCT ATGGCTTCTT TTCTTTGCTG TCTGACATCA TCTCGTCTGA CGGTGATGAA 
GATGATGATG ATGGGGATGA GGACACTGAT AAAGGAGAAA TAGAGGAGCC TCCCTTGAAA 
CAAAAAGAAA TACACAAAGA AAAGGCTGAA AAGGAGGAGA AACCTGAAAG GAAAATACTA 
GCCAAAGTTG CACACAGAGA AAAAGAAAAA GTAAAAGAAA AAGAAAAATC TGAAAAGAAA 
GCAACTCACA AGGAAAAAAT TGAGAAAAAA GAAAAACCAG AAACAAAGAC AATGGCAAAA 
GAAGAGAGGA AAGCCAAGAC TGAAGAGAAG ATCAAAAAGG AAGTGAAAGG TGGAAAACAA 
GAGAAAGTGA AGCCAACAGC TGCAAAAGTC AAAGAAGTAC AGAAAACACC ACCTAAGGCC 
AAAGAGAAGG AGGGGAAAGA GACTGCGGCT GTGGCCAAGC ATGAACAGAA AGATCAGTAT 
GCATTCTGTC GATATATGAT TGACATGTTT GTCCATGGGG ATTTAAGACC AGGACAAAGC 
CCAGCCTTAC CACCTCCATT ACCAACAGTA CAAGCTTCCA GACCCACTCC GGCATCACCT 
ACTCTTGAAG GAAAAGAAGA GGAGGAAAAG AAGAAAGCTG AGAAGAAAGT AACTTCTGAA 
ACAAAAAAGA AAGAAAAAGA AGATGTCAAA AAGAAGTCGG ACAAGGACAC TGCCATTGAT 
GTGGAAAAAA AAGAGCCAGG GAAAGCTCCT GAAACCAAAC AAGGGACTAT AAAAGTTGTG 
GCACAAGCAG CGGCTAAAAA AGATGAAAAG AAGGAAGATT CCAAGAAAAC AAAAACACCT 
GTAGAAGAAC ATCCCAAGGG AAAAAAACAG GAAAAGAAAG AAAAATACGT GGAACCAGCA 
AAGTCGTCAA AGAAGGAACA TTCAGCTCCA AGTGAAAAAC AAGTAAAAGC AAAAACTGAA 
CGAGCCAAAG AAGAGACTAG TGCTGCCTCA ACTAAAAAAG CTGTACCTGG AAAGAAGGAA 
GAGAAAACAA CCAAGACAGT GGAGCAAGAA ATTAGAAAAG AAAAGTCCGG GAAGACTTCT 
ACAGCTTCGA AGGACAAAGA GCCTGAAATT AAAAAAGATG AAAAGATGCC CAAAGCAGAC 
AAAGAAGTCA AGCCTAAGCC TCCACAGTCA CAAGTAAAGA AAGAAGAGAA ATCAGAGTCA 
CAAGTTAAAA AAGAAGCAAA ACCAGAACAA GACATAGCAA AACCAGAAAA GACTGTTTCT 
CATGGGAAAC CAGAAGAAAA AGTTGTCAAG CAGGTAAAAG CTACAGAAAA AGCAGCTATA 
GAAAAAACAG TCAAGCCCAA ACCAGCAAAA AAAGCTGAAC ATCAAGAAAA AGAATCTCCA 
ACTATAAAAA CAGACAAACC CAAACCAACC TCAAAGGAAA CACCAGAAGT CACAGAATCA 
GGGAAGAAGA AAATTGAAAA ATCTGAAAAA GAAAGTAAAG AAAAAGCAGA AATGAAACAT 
CTTAAAGAAG AAAAAGTCTC AACAAGAAAA GAAAGTCTTC AATCACACAA CGTGACAAAA 
GCAGAAAAAC CTGCAAGAGT ATCAAGAGAA GATTTGGAAG ATGTATCAGC TTCAAAGAAA 
GCTAAAGAAG AAGCTGAAGA TGTGTCTTCC ACAAAGAGGC AAAAAAGCCC CATCAGTTTC 
TTCCAGTGTG TCTACTTGGA TGGATACAAT GGCTACGGAT TTCAGTTTCC TGTCACTCCC 
GCATACCGTC CTGGAGAGAG CTCTGGCCAA CCAAGTTCTC CAGGACAGAA GCAACAAGGA 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001003154.2
CDS	216..2321
Translation	MTEITAEGNASTTTTVIDSKNGSVPKSPGKVLKRTVTEDIVTTFSSPAAWLLVIALIITWSAVAVVMFDLVDYKNFSASSLSKIGSDPLKLVHDAVEETTDWVYGFFSLLSDIISSDGDEDDDDGDEDTDKGEIEEPPLKQKEIHKEKAEKEEKPERKILAKVAHREKEKVKEKEKSEKKATHKEKIEKKEKPETKTMAKEERKAKTEEKIKKEVKGGKQEKVKPTAAKVKEVQKTPPKAKEKEGKETAAVAKHEQKDQYAFCRYMIDMFVHGDLRPGQSPALPPPLPTVQASRPTPASPTLEGKEEEEKKKAEKKVTSETKKKEKEDVKKKSDKDTAIDVEKKEPGKAPETKQGTIKVVAQAAAKKDEKKEDSKKTKTPVEEHPKGKKQEKKEKYVEPAKSSKKEHSAPSEKQVKAKTERAKEETSAASTKKAVPGKKEEKTTKTVEQEIRKEKSGKTSTASKDKEPEIKKDEKMPKADKEVKPKPPQSQVKKEEKSESQVKKEAKPEQDIAKPEKTVSHGKPEEKVVKQVKATEKAAIEKTVKPKPAKKAEHQEKESPTIKTDKPKPTSKETPEVTESGKKKIEKSEKESKEKAEMKHLKEEKVSTRKESLQSHNVTKAEKPARVSREDLEDVSASKKAKEEAEDVSSTKRQKSPISFFQCVYLDGYNGYGFQFPVTPAYRPGESSGQPSSPGQKQQGQ

Target ORF information:

RefSeq Version	NM_001003154.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris triadin (TRDN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001003154.2

ORF Insert Sequence:

ATGACTGAGA TCACTGCTGA AGGAAATGCA TCTACTACCA CAACTGTGAT CGACAGCAAA 
AATGGATCTG TGCCCAAATC CCCTGGAAAA GTGCTGAAGA GGACAGTCAC AGAAGACATA 
GTGACAACAT TCAGCTCCCC AGCAGCCTGG CTTCTGGTCA TTGCTCTGAT CATCACATGG 
TCAGCTGTTG CCGTTGTTAT GTTTGACCTA GTGGATTACA AAAACTTTTC AGCGAGCTCT 
CTTTCCAAGA TTGGCTCAGA TCCTCTAAAA CTTGTACATG ATGCTGTGGA AGAAACCACG 
GACTGGGTCT ATGGCTTCTT TTCTTTGCTG TCTGACATCA TCTCGTCTGA CGGTGATGAA 
GATGATGATG ATGGGGATGA GGACACTGAT AAAGGAGAAA TAGAGGAGCC TCCCTTGAAA 
CAAAAAGAAA TACACAAAGA AAAGGCTGAA AAGGAGGAGA AACCTGAAAG GAAAATACTA 
GCCAAAGTTG CACACAGAGA AAAAGAAAAA GTAAAAGAAA AAGAAAAATC TGAAAAGAAA 
GCAACTCACA AGGAAAAAAT TGAGAAAAAA GAAAAACCAG AAACAAAGAC AATGGCAAAA 
GAAGAGAGGA AAGCCAAGAC TGAAGAGAAG ATCAAAAAGG AAGTGAAAGG TGGAAAACAA 
GAGAAAGTGA AGCCAACAGC TGCAAAAGTC AAAGAAGTAC AGAAAACACC ACCTAAGGCC 
AAAGAGAAGG AGGGGAAAGA GACTGCGGCT GTGGCCAAGC ATGAACAGAA AGATCAGTAT 
GCATTCTGTC GATATATGAT TGACATGTTT GTCCATGGGG ATTTAAGACC AGGACAAAGC 
CCAGCCTTAC CACCTCCATT ACCAACAGTA CAAGCTTCCA GACCCACTCC GGCATCACCT 
ACTCTTGAAG GAAAAGAAGA GGAGGAAAAG AAGAAAGCTG AGAAGAAAGT AACTTCTGAA 
ACAAAAAAGA AAGAAAAAGA AGATGTCAAA AAGAAGTCGG ACAAGGACAC TGCCATTGAT 
GTGGAAAAAA AAGAGCCAGG GAAAGCTCCT GAAACCAAAC AAGGGACTAT AAAAGTTGTG 
GCACAAGCAG CGGCTAAAAA AGATGAAAAG AAGGAAGATT CCAAGAAAAC AAAAACACCT 
GTAGAAGAAC ATCCCAAGGG AAAAAAACAG GAAAAGAAAG AAAAATACGT GGAACCAGCA 
AAGTCGTCAA AGAAGGAACA TTCAGCTCCA AGTGAAAAAC AAGTAAAAGC AAAAACTGAA 
CGAGCCAAAG AAGAGACTAG TGCTGCCTCA ACTAAAAAAG CTGTACCTGG AAAGAAGGAA 
GAGAAAACAA CCAAGACAGT GGAGCAAGAA ATTAGAAAAG AAAAGTCCGG GAAGACTTCT 
ACAGCTTCGA AGGACAAAGA GCCTGAAATT AAAAAAGATG AAAAGATGCC CAAAGCAGAC 
AAAGAAGTCA AGCCTAAGCC TCCACAGTCA CAAGTAAAGA AAGAAGAGAA ATCAGAGTCA 
CAAGTTAAAA AAGAAGCAAA ACCAGAACAA GACATAGCAA AACCAGAAAA GACTGTTTCT 
CATGGGAAAC CAGAAGAAAA AGTTGTCAAG CAGGTAAAAG CTACAGAAAA AGCAGCTATA 
GAAAAAACAG TCAAGCCCAA ACCAGCAAAA AAAGCTGAAC ATCAAGAAAA AGAATCTCCA 
ACTATAAAAA CAGACAAACC CAAACCAACC TCAAAGGAAA CACCAGAAGT CACAGAATCA 
GGGAAGAAGA AAATTGAAAA ATCTGAAAAA GAAAGTAAAG AAAAAGCAGA AATGAAACAT 
CTTAAAGAAG AAAAAGTCTC AACAAGAAAA GAAAGTCTTC AATCACACAA CGTGACAAAA 
GCAGAAAAAC CTGCAAGAGT ATCAAGAGAA GATTTGGAAG ATGTATCAGC TTCAAAGAAA 
GCTAAAGAAG AAGCTGAAGA TGTGTCTTCC ACAAAGAGGC AAAAAAGCCC CATCAGTTTC 
TTCCAGTGTG TCTACTTGGA TGGATACAAT GGCTACGGAT TTCAGTTTCC TGTCACTCCC 
GCATACCGTC CTGGAGAGAG CTCTGGCCAA CCAAGTTCTC CAGGACAGAA GCAACAAGGA 
CAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inefficient glycosylation leads to high steady-state levels of actively degrading cardiac triadin-1.
The Journal of biological chemistry283(4)1929-35(2008 Jan)
Milstein ML,McFarland TP,Marsh JD,Cala SE

Triadin overexpression stimulates excitation-contraction coupling and increases predisposition to cellular arrhythmia in cardiac myocytes.
Circulation research96(6)651-8(2005 Apr)
Terentyev D,Cala SE,Houle TD,Viatchenko-Karpinski S,Gyorke I,Terentyeva R,Williams SC,Gyorke S

The role of calsequestrin, triadin, and junctin in conferring cardiac ryanodine receptor responsiveness to luminal calcium.
Biophysical journal86(4)2121-8(2004 Apr)
Gy?rke I,Hester N,Jones LR,Gy?rke S

Cardiac hypertrophy and impaired relaxation in transgenic mice overexpressing triadin 1.
The Journal of biological chemistry276(6)4142-9(2001 Feb)
Kirchhefer U,Neumann J,Baba HA,Begrow F,Kobayashi YM,Reinke U,Schmitz W,Jones LR

Identification of triadin 1 as the predominant triadin isoform expressed in mammalian myocardium.
The Journal of biological chemistry274(40)28660-8(1999 Oct)
Kobayashi YM,Jones LR