crim1 cDNA ORF clone, Callorhinchus milii(elephant shark)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	103181761
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Callorhinchus milii(elephant shark)

mRNA and Protein(s)

mRNA	Protein	Name
XM_007898435.1	XP_007896626.1	cysteine-rich motor neuron 1 protein isoform X1
XM_007898436.1	XP_007896627.1	cysteine-rich motor neuron 1 protein isoform X2

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa379760	XM_007898435.1 Latest version!	Callorhinchus milii cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OCa379761	XM_007898436.1 Latest version!	Callorhinchus milii cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_007898435.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007896626.1 Callorhinchus milii cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCa379760
Clone ID Related Accession (Same CDS sequence)	XM_007898435.1
Accession Version	XM_007898435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890115.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTTT CAGATGAGAA CTGGGAGGAC GACCAACTTC TTGGACTTGA GCCCTGCAAT 
GAAAACCTCA TAACAGGATG CAACATAATC AATGGGAAAT GTGAATGTGA TTCGATTCGG 
ACCTGTAATA ACCCATTTGT ATTTCCCAGT CGGGAAACCT GTCTGTCAGC GTTAAAGAGA 
ATTGAAGAGG AAAAGCCAGA CTGTTCCAAA GCCAGGTGTG AAGTGCAGTT TTCCCCTCGC 
TGCCCGGAGG ACGCCATCCT GATCGAAGGC TATGCCCCAC CTGGCGAGTG TTGCCCTCTG 
CCAAGCCGCT GTGTGTGCAA ACCAGCTGGT TGTTTGAGAA AAGTCTGTCA GCCCGGCTAC 
CAGAGTATTC TAGTTTCCAA AGCATCGGGG AAACCAGGGG AATGTTGCGA CCTGTACGAG 
TGCAAAAAAG TGTATAACGT GGACTGCAGC ACCGTGGAGT GCCCACCCGT ACAGCAAGTG 
GTCTGCCCGC CCGACAGCTT TGAGACACGG ACCCGGCTGA CTGCCGACGG CTGCTGTACT 
CTCCCGACTC GGTGTGAATG TTTGCTGGGA ATCTGCCGGG TCCCTAAATG TGAGGCTGGC 
GCAGTGCCCC GCATCATTTC TCCTGGAGAC GGCAGCCCTG GTCGGTGCTG TGATGTGTTT 
CAGTGCGTCA ATGAGACGAA GCCCACTTGT ATATTCAATG GTGTGGAATA TTATGACGGG 
AACATGTTCC GGATGGACGC TTGCCGCTTC TGCCGGTGTC AGGGAGGAGT CTCTATCTGT 
TTTTCAGCGC AGTGCGGGGA GTTGCACTGC GACCGGTACT ATGTGCCAGA AGGAGAATGC 
TGCCCAGTGT GTGAAGACCC GATATACCCA GTGCATAATC CTGCCGGCTG TTATGCCAAT 
GGGCAGATCC GCGCCCACGG TGACCGGTGG CGTGAAGACG ATTGCACCTT CTGTCAGTGC 
ATTAATGGGG AACCCCACTG TGTGGCCACA GCGTGTGGGC AGAGCTGCCT GAACCCTGTG 
AAGATTCCTG GTGAATGCTG CCCCGTCTGC GAAGAGCCCA CCTACATTAC CATCGGCCCT 
CCTACATGTG AGCTACTGGT GAACTGCACA TTGACGGAGA AGGACTGCAT CTTCGGCTTC 
AAGCTGGACC GGAATGGATG CCGCACGTGC CAGTGCAAAA CCAGAGCAGA GCTGTGCACT 
GGGCTGATAT CAGGCTGTGC CCTGGACTGT CCTTTTGGTT TGCAGACTGG CCCACACGGT 
TGTGAGATTT GCCAGTGCCG CCCAAGGCCA AAGAAGTGCA AACCAGCAGC TTGCGACAAA 
TACTGCCCTT TTGGATACAC GAAGAACAAA CATGGCTGTG ATGTCTGTCG CTGCAAGAAG 
TGTCCAGAGA TCCCATGTGA CAAGTACTGT TCAATGGGCT TTGAGCAGGA TATTTACGGC 
TGTCTTATCT GCAAATGCAG AGGTAAAGGT GCGGGAGTGG CGGAAGATGT GCCGACAACC 
ATGGTTCCTC CAGTCAAAAC TGGCTCTTGC TTGTCCATGG ACGGCCGACG GCACGAGAGT 
GGTGAGAGCT GGCATGATGG GTGCCGCGAG TGCTTTTGCT ACAGTGGACG TGAGATGTGC 
GCCCTCATCA CCTGCTCCGT GCCCAACTGT GGGAATCCTA CCATCCGGCC TGGACAATGC 
TGCCCATCAT GCCCGGAGGA TTCTGGCCTT CGGAAGCCTG AGCTGAGCGA CACATCTATC 
TGCCACGCTC CTGGCGGTGA ATATTTTGTG GAAGGAGAAA CGTGGAACAT CGACGCTTGC 
ACCCAGTGCA CGTGCCACAT TGGCCGGGTG CTGTGCGACA CCGAGGTCTG CCCGCCAATC 
CTCTGCCAGA ACCCCATCCG CACCCAGGAC TCGTGCTGCC CACAGTGCCC AGATGAGCCC 
CTTCAGCCTT TGTCGCCGAG CAACAAAAGC ATGCCCAGTT ACTGCAAAAG CGAGGATGGA 
GATATCTACC TCGCTGCCGA ATCCTGGAAA CCCAACGTCT GCTCCAGCTG CATATGCATG 
GATGGGGTCA TCAACTGCTT CTCAGAGTCG TGCCCTCCTG TGACGTGTGC CCGACCTGTC 
CTGCGGAAAG GGCAGTGCTG TCCATACTGC ATTGAGGAGA GCATCCCAAA GAAGGTGGTG 
TGTCACTTCA ACGAAAAGAC ATACGTAGAC GAGGAGAGGT GGGACATTGA CAGCTGCACC 
CACTGTTACT GCCTGCAAGG TCAGACACTG TGCTCGACAG TCAGTTGCCC TCCGCTTGCC 
TGCCTGGAGC CAGTCTACGT AGAAGGGAGC TGCTGTCCTG TCTGTCCAGA CCTCTACATG 
CCCGAGCCAA CCAACATCCC GATAGAAAAA TTTGACCCAA GGGGAGAGAT CGAAGTGGAA 
GCCCCATTCT GGCCGACACC TGGTGAAAAC GAAATTCCCT CACACCTTCG AGACATGGGC 
AGTGAGAACC TTCGTGTTAA TGCCCACGGC GGAGATTATC CCCGGGACAA CGAGGATTCT 
GCTATACACT CGGTGGCGTG GGTGGCCATT CCGGTCATGA TCGCCCTGAT TCTTGTCATA 
GCTCTGCTGC TGGTTAATCA GAAGAAACGG TGGATCCCAG TGCCCTGCTA CAGAGCTCCT 
ACAAAGCCCT CCTGTCTGAA TAATCAGCTG GTTTATGTGG ACTGTAAGAA AGGAACCATG 
GTTCAAGTGG ATAGCTCCCA GAGAATGCTG AGGATGGCAG ACCCAGACTC CAGACACAGT 
GGTTTCTATA GCATGCCAAA CACAAAGCAG AACAACTTGC AGGTGGACAG CTTCTACCAG 
ACAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007896626.1
CDS	333..3161
Translation	MFLSDENWEDDQLLGLEPCNENLITGCNIINGKCECDSIRTCNNPFVFPSRETCLSALKRIEEEKPDCSKARCEVQFSPRCPEDAILIEGYAPPGECCPLPSRCVCKPAGCLRKVCQPGYQSILVSKASGKPGECCDLYECKKVYNVDCSTVECPPVQQVVCPPDSFETRTRLTADGCCTLPTRCECLLGICRVPKCEAGAVPRIISPGDGSPGRCCDVFQCVNETKPTCIFNGVEYYDGNMFRMDACRFCRCQGGVSICFSAQCGELHCDRYYVPEGECCPVCEDPIYPVHNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCLNPVKIPGECCPVCEEPTYITIGPPTCELLVNCTLTEKDCIFGFKLDRNGCRTCQCKTRAELCTGLISGCALDCPFGLQTGPHGCEICQCRPRPKKCKPAACDKYCPFGYTKNKHGCDVCRCKKCPEIPCDKYCSMGFEQDIYGCLICKCRGKGAGVAEDVPTTMVPPVKTGSCLSMDGRRHESGESWHDGCRECFCYSGREMCALITCSVPNCGNPTIRPGQCCPSCPEDSGLRKPELSDTSICHAPGGEYFVEGETWNIDACTQCTCHIGRVLCDTEVCPPILCQNPIRTQDSCCPQCPDEPLQPLSPSNKSMPSYCKSEDGDIYLAAESWKPNVCSSCICMDGVINCFSESCPPVTCARPVLRKGQCCPYCIEESIPKKVVCHFNEKTYVDEERWDIDSCTHCYCLQGQTLCSTVSCPPLACLEPVYVEGSCCPVCPDLYMPEPTNIPIEKFDPRGEIEVEAPFWPTPGENEIPSHLRDMGSENLRVNAHGGDYPRDNEDSAIHSVAWVAIPVMIALILVIALLLVNQKKRWIPVPCYRAPTKPSCLNNQLVYVDCKKGTMVQVDSSQRMLRMADPDSRHSGFYSMPNTKQNNLQVDSFYQTV

Target ORF information:

RefSeq Version	XM_007898435.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007898435.1

ORF Insert Sequence:

ATGTTTCTTT CAGATGAGAA CTGGGAGGAC GACCAACTTC TTGGACTTGA GCCCTGCAAT 
GAAAACCTCA TAACAGGATG CAACATAATC AATGGGAAAT GTGAATGTGA TTCGATTCGG 
ACCTGTAATA ACCCATTTGT ATTTCCCAGT CGGGAAACCT GTCTGTCAGC GTTAAAGAGA 
ATTGAAGAGG AAAAGCCAGA CTGTTCCAAA GCCAGGTGTG AAGTGCAGTT TTCCCCTCGC 
TGCCCGGAGG ACGCCATCCT GATCGAAGGC TATGCCCCAC CTGGCGAGTG TTGCCCTCTG 
CCAAGCCGCT GTGTGTGCAA ACCAGCTGGT TGTTTGAGAA AAGTCTGTCA GCCCGGCTAC 
CAGAGTATTC TAGTTTCCAA AGCATCGGGG AAACCAGGGG AATGTTGCGA CCTGTACGAG 
TGCAAAAAAG TGTATAACGT GGACTGCAGC ACCGTGGAGT GCCCACCCGT ACAGCAAGTG 
GTCTGCCCGC CCGACAGCTT TGAGACACGG ACCCGGCTGA CTGCCGACGG CTGCTGTACT 
CTCCCGACTC GGTGTGAATG TTTGCTGGGA ATCTGCCGGG TCCCTAAATG TGAGGCTGGC 
GCAGTGCCCC GCATCATTTC TCCTGGAGAC GGCAGCCCTG GTCGGTGCTG TGATGTGTTT 
CAGTGCGTCA ATGAGACGAA GCCCACTTGT ATATTCAATG GTGTGGAATA TTATGACGGG 
AACATGTTCC GGATGGACGC TTGCCGCTTC TGCCGGTGTC AGGGAGGAGT CTCTATCTGT 
TTTTCAGCGC AGTGCGGGGA GTTGCACTGC GACCGGTACT ATGTGCCAGA AGGAGAATGC 
TGCCCAGTGT GTGAAGACCC GATATACCCA GTGCATAATC CTGCCGGCTG TTATGCCAAT 
GGGCAGATCC GCGCCCACGG TGACCGGTGG CGTGAAGACG ATTGCACCTT CTGTCAGTGC 
ATTAATGGGG AACCCCACTG TGTGGCCACA GCGTGTGGGC AGAGCTGCCT GAACCCTGTG 
AAGATTCCTG GTGAATGCTG CCCCGTCTGC GAAGAGCCCA CCTACATTAC CATCGGCCCT 
CCTACATGTG AGCTACTGGT GAACTGCACA TTGACGGAGA AGGACTGCAT CTTCGGCTTC 
AAGCTGGACC GGAATGGATG CCGCACGTGC CAGTGCAAAA CCAGAGCAGA GCTGTGCACT 
GGGCTGATAT CAGGCTGTGC CCTGGACTGT CCTTTTGGTT TGCAGACTGG CCCACACGGT 
TGTGAGATTT GCCAGTGCCG CCCAAGGCCA AAGAAGTGCA AACCAGCAGC TTGCGACAAA 
TACTGCCCTT TTGGATACAC GAAGAACAAA CATGGCTGTG ATGTCTGTCG CTGCAAGAAG 
TGTCCAGAGA TCCCATGTGA CAAGTACTGT TCAATGGGCT TTGAGCAGGA TATTTACGGC 
TGTCTTATCT GCAAATGCAG AGGTAAAGGT GCGGGAGTGG CGGAAGATGT GCCGACAACC 
ATGGTTCCTC CAGTCAAAAC TGGCTCTTGC TTGTCCATGG ACGGCCGACG GCACGAGAGT 
GGTGAGAGCT GGCATGATGG GTGCCGCGAG TGCTTTTGCT ACAGTGGACG TGAGATGTGC 
GCCCTCATCA CCTGCTCCGT GCCCAACTGT GGGAATCCTA CCATCCGGCC TGGACAATGC 
TGCCCATCAT GCCCGGAGGA TTCTGGCCTT CGGAAGCCTG AGCTGAGCGA CACATCTATC 
TGCCACGCTC CTGGCGGTGA ATATTTTGTG GAAGGAGAAA CGTGGAACAT CGACGCTTGC 
ACCCAGTGCA CGTGCCACAT TGGCCGGGTG CTGTGCGACA CCGAGGTCTG CCCGCCAATC 
CTCTGCCAGA ACCCCATCCG CACCCAGGAC TCGTGCTGCC CACAGTGCCC AGATGAGCCC 
CTTCAGCCTT TGTCGCCGAG CAACAAAAGC ATGCCCAGTT ACTGCAAAAG CGAGGATGGA 
GATATCTACC TCGCTGCCGA ATCCTGGAAA CCCAACGTCT GCTCCAGCTG CATATGCATG 
GATGGGGTCA TCAACTGCTT CTCAGAGTCG TGCCCTCCTG TGACGTGTGC CCGACCTGTC 
CTGCGGAAAG GGCAGTGCTG TCCATACTGC ATTGAGGAGA GCATCCCAAA GAAGGTGGTG 
TGTCACTTCA ACGAAAAGAC ATACGTAGAC GAGGAGAGGT GGGACATTGA CAGCTGCACC 
CACTGTTACT GCCTGCAAGG TCAGACACTG TGCTCGACAG TCAGTTGCCC TCCGCTTGCC 
TGCCTGGAGC CAGTCTACGT AGAAGGGAGC TGCTGTCCTG TCTGTCCAGA CCTCTACATG 
CCCGAGCCAA CCAACATCCC GATAGAAAAA TTTGACCCAA GGGGAGAGAT CGAAGTGGAA 
GCCCCATTCT GGCCGACACC TGGTGAAAAC GAAATTCCCT CACACCTTCG AGACATGGGC 
AGTGAGAACC TTCGTGTTAA TGCCCACGGC GGAGATTATC CCCGGGACAA CGAGGATTCT 
GCTATACACT CGGTGGCGTG GGTGGCCATT CCGGTCATGA TCGCCCTGAT TCTTGTCATA 
GCTCTGCTGC TGGTTAATCA GAAGAAACGG TGGATCCCAG TGCCCTGCTA CAGAGCTCCT 
ACAAAGCCCT CCTGTCTGAA TAATCAGCTG GTTTATGTGG ACTGTAAGAA AGGAACCATG 
GTTCAAGTGG ATAGCTCCCA GAGAATGCTG AGGATGGCAG ACCCAGACTC CAGACACAGT 
GGTTTCTATA GCATGCCAAA CACAAAGCAG AACAACTTGC AGGTGGACAG CTTCTACCAG 
ACAGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_007898436.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> XP_007896627.1 Callorhinchus milii cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCa379761
Clone ID Related Accession (Same CDS sequence)	XM_007898436.1
Accession Version	XM_007898436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2805bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-05-08
Organism	Callorhinchus milii(elephant shark)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_006890115.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Callorhinchus milii Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTTT CAGATGAGAA CTGGGAGGAC GACCAACTTC TTGGACTTGA GCCCTGCAAT 
GAAAACCTCA TAACAGGATG CAACATAATC AATGGGAAAT GTGAATGTGA TTCGATTCGG 
ACCTGTAATA ACCCATTTGT ATTTCCCAGT CGGGAAACCT GTCTGTCAGC GTTAAAGAGA 
ATTGAAGAGG AAAAGCCAGA CTGTTCCAAA GCCAGGTGTG AAGTGCAGTT TTCCCCTCGC 
TGCCCGGAGG ACGCCATCCT GATCGAAGGC TATGCCCCAC CTGGCGAGTG TTGCCCTCTG 
CCAAGCCGCT GTGTGTGCAA ACCAGCTGGT TGTTTGAGAA AAGTCTGTCA GCCCGGCTAC 
CAGAGTATTC TAGTTTCCAA AGCATCGGGG AAACCAGGGG AATGTTGCGA CCTGTACGAG 
TGCAAAAAAG TGTATAACGT GGACTGCAGC ACCGTGGAGT GCCCACCCGT ACAGCAAGTG 
GTCTGCCCGC CCGACAGCTT TGAGACACGG ACCCGGCTGA CTGCCGACGG CTGCTGTACT 
CTCCCGACTC GGTGTGAATG TTTGCTGGGA ATCTGCCGGG TCCCTAAATG TGAGGCTGGC 
GCAGTGCCCC GCATCATTTC TCCTGGAGAC GGCAGCCCTG GTCGGTGCTG TGATGTGTTT 
CAGTGCGTCA ATGAGACGAA GCCCACTTGT ATATTCAATG GTGTGGAATA TTATGACGGG 
AACATGTTCC GGATGGACGC TTGCCGCTTC TGCCGGTGTC AGGGAGGAGT CTCTATCTGT 
TTTTCAGCGC AGTGCGGGGA GTTGCACTGC GACCGGTACT ATGTGCCAGA AGGAGAATGC 
TGCCCAGTGT GTGAAGACCC GATATACCCA GTGCATAATC CTGCCGGCTG TTATGCCAAT 
GGGCAGATCC GCGCCCACGG TGACCGGTGG CGTGAAGACG ATTGCACCTT CTGTCAGTGC 
ATTAATGGGG AACCCCACTG TGTGGCCACA GCGTGTGGGC AGAGCTGCCT GAACCCTGTG 
AAGATTCCTG GTGAATGCTG CCCCGTCTGC GAAGAGCCCA CCTACATTAC CATCGGCCCT 
CCTACATGTG AGCTACTGGT GAACTGCACA TTGACGGAGA AGGACTGCAT CTTCGGCTTC 
AAGCTGGACC GGAATGGATG CCGCACGTGC CAGTGCAAAA CCAGAGCAGA GCTGTGCACT 
GGGCTGATAT CAGGCTGTGC CCTGGACTGT CCTTTTGGTT TGCAGACTGG CCCACACGGT 
TGTGAGATTT GCCAGTGCCG CCCAAGGCCA AAGAAGTGCA AACCAGCAGC TTGCGACAAA 
TACTGCCCTT TTGGATACAC GAAGAACAAA CATGGCTGTG ATGTCTGTCG CTGCAAGAAG 
TGTCCAGAGA TCCCATGTGA CAAGTACTGT TCAATGGGCT TTGAGCAGGA TATTTACGGC 
TGTCTTATCT GCAAATGCAG AGATGTGCCG ACAACCATGG TTCCTCCAGT CAAAACTGGC 
TCTTGCTTGT CCATGGACGG CCGACGGCAC GAGAGTGGTG AGAGCTGGCA TGATGGGTGC 
CGCGAGTGCT TTTGCTACAG TGGACGTGAG ATGTGCGCCC TCATCACCTG CTCCGTGCCC 
AACTGTGGGA ATCCTACCAT CCGGCCTGGA CAATGCTGCC CATCATGCCC GGAGGATTCT 
GGCCTTCGGA AGCCTGAGCT GAGCGACACA TCTATCTGCC ACGCTCCTGG CGGTGAATAT 
TTTGTGGAAG GAGAAACGTG GAACATCGAC GCTTGCACCC AGTGCACGTG CCACATTGGC 
CGGGTGCTGT GCGACACCGA GGTCTGCCCG CCAATCCTCT GCCAGAACCC CATCCGCACC 
CAGGACTCGT GCTGCCCACA GTGCCCAGAT GAGCCCCTTC AGCCTTTGTC GCCGAGCAAC 
AAAAGCATGC CCAGTTACTG CAAAAGCGAG GATGGAGATA TCTACCTCGC TGCCGAATCC 
TGGAAACCCA ACGTCTGCTC CAGCTGCATA TGCATGGATG GGGTCATCAA CTGCTTCTCA 
GAGTCGTGCC CTCCTGTGAC GTGTGCCCGA CCTGTCCTGC GGAAAGGGCA GTGCTGTCCA 
TACTGCATTG AGGAGAGCAT CCCAAAGAAG GTGGTGTGTC ACTTCAACGA AAAGACATAC 
GTAGACGAGG AGAGGTGGGA CATTGACAGC TGCACCCACT GTTACTGCCT GCAAGGTCAG 
ACACTGTGCT CGACAGTCAG TTGCCCTCCG CTTGCCTGCC TGGAGCCAGT CTACGTAGAA 
GGGAGCTGCT GTCCTGTCTG TCCAGACCTC TACATGCCCG AGCCAACCAA CATCCCGATA 
GAAAAATTTG ACCCAAGGGG AGAGATCGAA GTGGAAGCCC CATTCTGGCC GACACCTGGT 
GAAAACGAAA TTCCCTCACA CCTTCGAGAC ATGGGCAGTG AGAACCTTCG TGTTAATGCC 
CACGGCGGAG ATTATCCCCG GGACAACGAG GATTCTGCTA TACACTCGGT GGCGTGGGTG 
GCCATTCCGG TCATGATCGC CCTGATTCTT GTCATAGCTC TGCTGCTGGT TAATCAGAAG 
AAACGGTGGA TCCCAGTGCC CTGCTACAGA GCTCCTACAA AGCCCTCCTG TCTGAATAAT 
CAGCTGGTTT ATGTGGACTG TAAGAAAGGA ACCATGGTTC AAGTGGATAG CTCCCAGAGA 
ATGCTGAGGA TGGCAGACCC AGACTCCAGA CACAGTGGTT TCTATAGCAT GCCAAACACA 
AAGCAGAACA ACTTGCAGGT GGACAGCTTC TACCAGACAG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_007896627.1
CDS	333..3137
Translation	MFLSDENWEDDQLLGLEPCNENLITGCNIINGKCECDSIRTCNNPFVFPSRETCLSALKRIEEEKPDCSKARCEVQFSPRCPEDAILIEGYAPPGECCPLPSRCVCKPAGCLRKVCQPGYQSILVSKASGKPGECCDLYECKKVYNVDCSTVECPPVQQVVCPPDSFETRTRLTADGCCTLPTRCECLLGICRVPKCEAGAVPRIISPGDGSPGRCCDVFQCVNETKPTCIFNGVEYYDGNMFRMDACRFCRCQGGVSICFSAQCGELHCDRYYVPEGECCPVCEDPIYPVHNPAGCYANGQIRAHGDRWREDDCTFCQCINGEPHCVATACGQSCLNPVKIPGECCPVCEEPTYITIGPPTCELLVNCTLTEKDCIFGFKLDRNGCRTCQCKTRAELCTGLISGCALDCPFGLQTGPHGCEICQCRPRPKKCKPAACDKYCPFGYTKNKHGCDVCRCKKCPEIPCDKYCSMGFEQDIYGCLICKCRDVPTTMVPPVKTGSCLSMDGRRHESGESWHDGCRECFCYSGREMCALITCSVPNCGNPTIRPGQCCPSCPEDSGLRKPELSDTSICHAPGGEYFVEGETWNIDACTQCTCHIGRVLCDTEVCPPILCQNPIRTQDSCCPQCPDEPLQPLSPSNKSMPSYCKSEDGDIYLAAESWKPNVCSSCICMDGVINCFSESCPPVTCARPVLRKGQCCPYCIEESIPKKVVCHFNEKTYVDEERWDIDSCTHCYCLQGQTLCSTVSCPPLACLEPVYVEGSCCPVCPDLYMPEPTNIPIEKFDPRGEIEVEAPFWPTPGENEIPSHLRDMGSENLRVNAHGGDYPRDNEDSAIHSVAWVAIPVMIALILVIALLLVNQKKRWIPVPCYRAPTKPSCLNNQLVYVDCKKGTMVQVDSSQRMLRMADPDSRHSGFYSMPNTKQNNLQVDSFYQTV

Target ORF information:

RefSeq Version	XM_007898436.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii cysteine rich transmembrane BMP regulator 1 (chordin-like) (crim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_007898436.1

ORF Insert Sequence:

ATGTTTCTTT CAGATGAGAA CTGGGAGGAC GACCAACTTC TTGGACTTGA GCCCTGCAAT 
GAAAACCTCA TAACAGGATG CAACATAATC AATGGGAAAT GTGAATGTGA TTCGATTCGG 
ACCTGTAATA ACCCATTTGT ATTTCCCAGT CGGGAAACCT GTCTGTCAGC GTTAAAGAGA 
ATTGAAGAGG AAAAGCCAGA CTGTTCCAAA GCCAGGTGTG AAGTGCAGTT TTCCCCTCGC 
TGCCCGGAGG ACGCCATCCT GATCGAAGGC TATGCCCCAC CTGGCGAGTG TTGCCCTCTG 
CCAAGCCGCT GTGTGTGCAA ACCAGCTGGT TGTTTGAGAA AAGTCTGTCA GCCCGGCTAC 
CAGAGTATTC TAGTTTCCAA AGCATCGGGG AAACCAGGGG AATGTTGCGA CCTGTACGAG 
TGCAAAAAAG TGTATAACGT GGACTGCAGC ACCGTGGAGT GCCCACCCGT ACAGCAAGTG 
GTCTGCCCGC CCGACAGCTT TGAGACACGG ACCCGGCTGA CTGCCGACGG CTGCTGTACT 
CTCCCGACTC GGTGTGAATG TTTGCTGGGA ATCTGCCGGG TCCCTAAATG TGAGGCTGGC 
GCAGTGCCCC GCATCATTTC TCCTGGAGAC GGCAGCCCTG GTCGGTGCTG TGATGTGTTT 
CAGTGCGTCA ATGAGACGAA GCCCACTTGT ATATTCAATG GTGTGGAATA TTATGACGGG 
AACATGTTCC GGATGGACGC TTGCCGCTTC TGCCGGTGTC AGGGAGGAGT CTCTATCTGT 
TTTTCAGCGC AGTGCGGGGA GTTGCACTGC GACCGGTACT ATGTGCCAGA AGGAGAATGC 
TGCCCAGTGT GTGAAGACCC GATATACCCA GTGCATAATC CTGCCGGCTG TTATGCCAAT 
GGGCAGATCC GCGCCCACGG TGACCGGTGG CGTGAAGACG ATTGCACCTT CTGTCAGTGC 
ATTAATGGGG AACCCCACTG TGTGGCCACA GCGTGTGGGC AGAGCTGCCT GAACCCTGTG 
AAGATTCCTG GTGAATGCTG CCCCGTCTGC GAAGAGCCCA CCTACATTAC CATCGGCCCT 
CCTACATGTG AGCTACTGGT GAACTGCACA TTGACGGAGA AGGACTGCAT CTTCGGCTTC 
AAGCTGGACC GGAATGGATG CCGCACGTGC CAGTGCAAAA CCAGAGCAGA GCTGTGCACT 
GGGCTGATAT CAGGCTGTGC CCTGGACTGT CCTTTTGGTT TGCAGACTGG CCCACACGGT 
TGTGAGATTT GCCAGTGCCG CCCAAGGCCA AAGAAGTGCA AACCAGCAGC TTGCGACAAA 
TACTGCCCTT TTGGATACAC GAAGAACAAA CATGGCTGTG ATGTCTGTCG CTGCAAGAAG 
TGTCCAGAGA TCCCATGTGA CAAGTACTGT TCAATGGGCT TTGAGCAGGA TATTTACGGC 
TGTCTTATCT GCAAATGCAG AGATGTGCCG ACAACCATGG TTCCTCCAGT CAAAACTGGC 
TCTTGCTTGT CCATGGACGG CCGACGGCAC GAGAGTGGTG AGAGCTGGCA TGATGGGTGC 
CGCGAGTGCT TTTGCTACAG TGGACGTGAG ATGTGCGCCC TCATCACCTG CTCCGTGCCC 
AACTGTGGGA ATCCTACCAT CCGGCCTGGA CAATGCTGCC CATCATGCCC GGAGGATTCT 
GGCCTTCGGA AGCCTGAGCT GAGCGACACA TCTATCTGCC ACGCTCCTGG CGGTGAATAT 
TTTGTGGAAG GAGAAACGTG GAACATCGAC GCTTGCACCC AGTGCACGTG CCACATTGGC 
CGGGTGCTGT GCGACACCGA GGTCTGCCCG CCAATCCTCT GCCAGAACCC CATCCGCACC 
CAGGACTCGT GCTGCCCACA GTGCCCAGAT GAGCCCCTTC AGCCTTTGTC GCCGAGCAAC 
AAAAGCATGC CCAGTTACTG CAAAAGCGAG GATGGAGATA TCTACCTCGC TGCCGAATCC 
TGGAAACCCA ACGTCTGCTC CAGCTGCATA TGCATGGATG GGGTCATCAA CTGCTTCTCA 
GAGTCGTGCC CTCCTGTGAC GTGTGCCCGA CCTGTCCTGC GGAAAGGGCA GTGCTGTCCA 
TACTGCATTG AGGAGAGCAT CCCAAAGAAG GTGGTGTGTC ACTTCAACGA AAAGACATAC 
GTAGACGAGG AGAGGTGGGA CATTGACAGC TGCACCCACT GTTACTGCCT GCAAGGTCAG 
ACACTGTGCT CGACAGTCAG TTGCCCTCCG CTTGCCTGCC TGGAGCCAGT CTACGTAGAA 
GGGAGCTGCT GTCCTGTCTG TCCAGACCTC TACATGCCCG AGCCAACCAA CATCCCGATA 
GAAAAATTTG ACCCAAGGGG AGAGATCGAA GTGGAAGCCC CATTCTGGCC GACACCTGGT 
GAAAACGAAA TTCCCTCACA CCTTCGAGAC ATGGGCAGTG AGAACCTTCG TGTTAATGCC 
CACGGCGGAG ATTATCCCCG GGACAACGAG GATTCTGCTA TACACTCGGT GGCGTGGGTG 
GCCATTCCGG TCATGATCGC CCTGATTCTT GTCATAGCTC TGCTGCTGGT TAATCAGAAG 
AAACGGTGGA TCCCAGTGCC CTGCTACAGA GCTCCTACAA AGCCCTCCTG TCTGAATAAT 
CAGCTGGTTT ATGTGGACTG TAAGAAAGGA ACCATGGTTC AAGTGGATAG CTCCCAGAGA 
ATGCTGAGGA TGGCAGACCC AGACTCCAGA CACAGTGGTT TCTATAGCAT GCCAAACACA 
AAGCAGAACA ACTTGCAGGT GGACAGCTTC TACCAGACAG TGTGA