rpl27a cDNA ORF clone, Callorhinchus milii(elephant shark)

Gene
Clones
gRNAs

Summary

Gene Symbol	rpl27a
Entrez Gene ID	103174921
Full Name	ribosomal protein L27a
Gene Type	protein-coding
Organism	Callorhinchus milii(elephant shark)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001292134.1	NP_001279063.1	ribosomal protein L27a

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Sequencing and analysis of full-length cDNAs, 5'-ESTs and 3'-ESTs from a cartilaginous fish, the elephant shark (Callorhinchus milii).
Tan YY, Kodzius R, Tay BH, Tay A, Brenner S, Venkatesh B
PloS one7(10)e47174(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following rpl27a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the rpl27a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCa82540	NM_001292134.1 Latest version!	Callorhinchus milii ribosomal protein L27a (rpl27a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

rpl27a ( NM_001292134.1 ) cDNA ORF clone, Callorhinchus milii(elephant shark) -> NP_001279063.1 Callorhinchus milii ribosomal protein L27a (rpl27a), mRNA.

Price & Availability↑

CloneID	OCa82540
Clone ID Related Accession (Same CDS sequence)	NM_001292134.1
Accession Version	NM_001292134.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-04
Organism	Callorhinchus milii(elephant shark)
Product	ribosomal protein L27a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JX212573.1. On May 23, 2014 this sequence version replaced XM_007887551.1. ##Evidence-Data-START## Transcript exon combination :: JX207741.1, JX212573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00188955, SAMN02699929 [ECO:0000348] ##Evidence-Data-END##

ATGCCATCCA AACTGAGAAA AACCAGGAAG CTACGAGGAC ATGTCAGCCA CGGCCATGGC 
CGTATTGGCA AGCACAGGAA GCATCCTGGT GGTCGGGGTA ATGCTGGTGG AATGCACCAC 
CACAGAATCA ACTTCGACAA ATATCATCCA GGTTACTTTG GAAAAGTTGG TATGAGACAT 
TTCCACTTGA AGAAGAATCA GTTGTTTAGT CCTACCATCA ATCTGGATAA GCTGTGGACA 
CTGGTCAGTG AACAGACCAG GGTAAACTAT GGCAAAAAGC CCGATGGACC AGCTCCCGTC 
ATTAATGTTG TACATGCTGG ATACTACAAA GTTCTGGGCA AGGGAAGGCT GCCTAAGCAA 
CCTCTCATTG TCAAGGCCAA GTTCTTCAGC AGGAAAGCAG AAGAGAAAAT CAAACAAGTT 
GGTGGGGCAT GTGTATTAAT GGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001279063.1
CDS	22..468
Translation	MPSKLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHFHLKKNQLFSPTINLDKLWTLVSEQTRVNYGKKPDGPAPVINVVHAGYYKVLGKGRLPKQPLIVKAKFFSRKAEEKIKQVGGACVLMA

Target ORF information:

RefSeq Version	NM_001292134.1
Organism	Callorhinchus milii(elephant shark)
Definition	Callorhinchus milii ribosomal protein L27a (rpl27a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001292134.1

ORF Insert Sequence:

ATGCCATCCA AACTGAGAAA AACCAGGAAG CTACGAGGAC ATGTCAGCCA CGGCCATGGC 
CGTATTGGCA AGCACAGGAA GCATCCTGGT GGTCGGGGTA ATGCTGGTGG AATGCACCAC 
CACAGAATCA ACTTCGACAA ATATCATCCA GGTTACTTTG GAAAAGTTGG TATGAGACAT 
TTCCACTTGA AGAAGAATCA GTTGTTTAGT CCTACCATCA ATCTGGATAA GCTGTGGACA 
CTGGTCAGTG AACAGACCAG GGTAAACTAT GGCAAAAAGC CCGATGGACC AGCTCCCGTC 
ATTAATGTTG TACATGCTGG ATACTACAAA GTTCTGGGCA AGGGAAGGCT GCCTAAGCAA 
CCTCTCATTG TCAAGGCCAA GTTCTTCAGC AGGAAAGCAG AAGAGAAAAT CAAACAAGTT 
GGTGGGGCAT GTGTATTAAT GGCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequencing and analysis of full-length cDNAs, 5'-ESTs and 3'-ESTs from a cartilaginous fish, the elephant shark (Callorhinchus milii).
PloS one7(10)e47174(2012)
Tan YY,Kodzius R,Tay BH,Tay A,Brenner S,Venkatesh B