NSMAF cDNA ORF clone, Buceros rhinoceros silvestris

Gene
Clones
gRNAs

Summary

Gene Symbol	NSMAF
Entrez Gene ID	104493843
Full Name	neutral sphingomyelinase activation associated factor
Gene Type	protein-coding
Organism	Buceros rhinoceros silvestris

mRNA and Protein(s)

mRNA	Protein	Name
XM_010136596.1	XP_010134898.1	protein FAN

Pathways from BioSystems
Gene Ontology

The following NSMAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSMAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu37960	XM_010136596.1 Latest version!	Buceros rhinoceros silvestris neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSMAF ( XM_010136596.1 ) cDNA ORF clone, Buceros rhinoceros silvestris -> XP_010134898.1 Buceros rhinoceros silvestris neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), mRNA.

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CloneID	OBu37960
Clone ID Related Accession (Same CDS sequence)	XM_010136596.1
Accession Version	XM_010136596.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Buceros rhinoceros silvestris
Product	protein FAN
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010413104.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Buceros rhinoceros silvestris Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGGTTCTCG AGAGGGACCC TGTTGATTCA GTAACTGTCA GAGGCTCTAC TTATTTACGT 
GGAAGAAGTC TGTTCTGTGA AGAGAAAAAG CTCTGGCTGT ACTCTAAGGA GAAGTTTTCA 
CTGCTTCTTC TTAACCTAGA AGAATACTAC TTTGAACAGC ACACAGCTAA TCATATTATA 
AATAAAGGCT GCAGAGATGA AAGAAAATTC AGAGGCTCTC TAAAAATCTG TTCCAAGTCA 
ATCATTTTTG AACCTGATGA CAATAACGAA CCCATTATCA AGATACCCCT GAGAGACTGC 
GTCAGTATAA AAGCCCCGGA AGATAATGAA GCAAATAACC TGTTCACACG GGACACCTCT 
GGAGGGATTT CTGTTGTGTG TAACCAGGCT TTTCTCATTA AAGAAAGGAA CATTATTGCA 
CCCTATAAAA CAGTTAGAGG TAAAACAGAA CACTTGTTCC AGCTGGATGT CGCTGGGAAA 
CTAGGAGACG TTGTGCAAAC TCTGCACCAG CTTTACAGGG CTTCTTGTCT AGACAAGATG 
GGGGATCAAG CTGCCATGAT TACTGCTATA TTGCAGTCGC GCTTGGCTAG AACTTCATTT 
GATAAAAACA GATTTCAAAG CATTTCTGAA ACGCTGCATA TGGAATGTAA AGCAGAAATG 
GTGACACCAC TGGTGACTAA TCCAGGGCAT GTGTGTGTTA CTGATGCTAA CTTGTACTTC 
CAGCCTCTTA ATGGATATCC TAAACCAGTC GTACAAATAA CACTGCAAAA TGTGCGTCGC 
ATCTATAAGA GACGACATGG GCTAATGCCG CTGGGACTAG AAGTATTTTG CACAGAAAAT 
GATTTATGTT CTGACATCTA CTTGAAATTC TACAACTATC AAGATCGAGA TGAGCTCTAT 
TTCCTCATTG CTACGTATAT AGAAAATCAT GTGACAGAGC ATACTGCTGA AAGTTATATG 
TTGCAGTGGC AGCGAGGACA TCTATCAAAT TACCAGTATC TTCTTCATCT CAACAATCTG 
GCTGACAGAA GCTGCAACGA TCTCTCACAG TATCCCGTAT TTCCCTGGAT CATAGCAGAC 
TACTCCAGTT CAGTGTTAGA TCTGACAAAG CCTGAAACAT TCCGTGACCT TAGTAAACCG 
ATTGGTGCCC TGAATAAGGA AAGGCTGGAT AGACTATTGA CACGTTATCA GGAAATGCCA 
GAGCCCAAGT TCATGTATGG GAGCCATTAT TCATCTCCGG GCTATGTGTT GTTTTACCTC 
GTCCGAGTTG CTCCAGAGTA CATGCTCTGT CTGCAGAATG GCAAGTTTGA TCACGCTGAC 
AGAATGTTCA ACAGTCTTGC AGAAACCTGG AAAAACTGTT TGGATGGTGC AACAGATTTC 
AAAGAGTTGA TTCCAGAATT TTATGAAAAT GATTCTAGTT TTCTAGTAAA CAGCTTGAAG 
TTGGACTTGG GAAAAAGGCA GGGTGGAAAG ATGGTTGAAG ATGTTGAGCT TCCCCCTTGG 
GCGTCAGGTC CTGATGACTT TCTTCAGAAG AGCCGAGAGG CTTTAGAAAG TCAGTATGTA 
TCAGAACATC TTCACGAATG GATTGACATA ATATTTGGCT ACAAGCAGAA AGGGAGTGAA 
GCGGTTGCAG CTCAAAACGT GTTTCATCCA TTAACTTACG AAGGAGGAGT GGATTTAAAC 
AGCATTATGG ACCCCAATGA GAAAGTAGCT CTGCTGACAC AGATCTTGGA ATTTGGACAG 
ACGCCAAAGC AGTTGTTTAC AATTCCTCAC CCTCAGAGGA TAATGCCGAA GTTGAAAAGC 
TTGTCTCGAA CATCGAGTCG CAGCATTTCC ATAACTGAGT CTCCAGTTTC TTCAAATGAA 
GAATCTTTTG AAGATTTGAC AGAAGAAAGC ATAACACTTG CTTGGAACAA TATTGCCAAA 
CTAAAATTAG ATGAGCAATA TAAAATTCAC AAAGAGGCCA TTACTGGAAT AGCAGTCACT 
TGCAGTGGGT CTTCTGTTCT CACTACATCC CAGGATTCAA CCTTAAAGAT GTTTTCCAAA 
GAATCAAAAA CAATCCAGAG AAGCGTGTCC TTTTCAAACA TGGCTCTGTC GTCTTGTCTC 
ATCCTACCAG GAGACACCAC TGTCATAAGT TCTTCGTGGG ACAATCATAT ATATTTTTAT 
TCAATTGCAT TTGGAAGGCA GCAGGACATC TTAATGGGGC ATGACGATGC AGTCAGCAAG 
ATCTGCTGGC GTGATGGGCG TTTATATTCC GCATCCTGGG ATTCCACCGT GAAGGTGTGG 
CATTGCGTCC CCGGAGAGGC CACGAGCAAT AAAAAACACC ACTTTGACCT GCTGGCAGAG 
CTGGAACACG ATGTTAGCGT GAATACAATC GACCTTAACG CTGCCAACAC TATGCTGGCA 
TCTGGAACCA AAGAGGGTAC CATCAGCGTT TGGGATGTTG CCACAGCAAC AGTGTTGCAC 
CAGCTGCCCT GTCATTCCGG GACAGTGTTT GACGCTGCTT TCAGTCCTGA CAGCAGACAC 
CTCCTCAGCG CAGGAGAGGA CTGTGCTTTT AAAGTAATAG ATGTTCAGAC TGGAATGCTC 
ATCTCTTCTG TAACGGCTGC GGAGCCACAG AGGTGCTTCA AATGGAATGG AAACACCATC 
TTATCAGGAA GTCAGTCTGG TGAATTACTG ATTTGGGACC TGCTGGGAGG AAAAGTTCTG 
GAAAGAATCA AAGGACATAC AGGTGCTGTA ACATCCCTGT GGATGAACGA ACAGTGCACC 
AGCATTATTA CAGGAGGGGA AGACAAACAG ATCATGTTCT GGAAGCTGCA GTACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010134898.1
CDS	1..2817
Translation	MVLERDPVDSVTVRGSTYLRGRSLFCEEKKLWLYSKEKFSLLLLNLEEYYFEQHTANHIINKGCRDERKFRGSLKICSKSIIFEPDDNNEPIIKIPLRDCVSIKAPEDNEANNLFTRDTSGGISVVCNQAFLIKERNIIAPYKTVRGKTEHLFQLDVAGKLGDVVQTLHQLYRASCLDKMGDQAAMITAILQSRLARTSFDKNRFQSISETLHMECKAEMVTPLVTNPGHVCVTDANLYFQPLNGYPKPVVQITLQNVRRIYKRRHGLMPLGLEVFCTENDLCSDIYLKFYNYQDRDELYFLIATYIENHVTEHTAESYMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWIIADYSSSVLDLTKPETFRDLSKPIGALNKERLDRLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRVAPEYMLCLQNGKFDHADRMFNSLAETWKNCLDGATDFKELIPEFYENDSSFLVNSLKLDLGKRQGGKMVEDVELPPWASGPDDFLQKSREALESQYVSEHLHEWIDIIFGYKQKGSEAVAAQNVFHPLTYEGGVDLNSIMDPNEKVALLTQILEFGQTPKQLFTIPHPQRIMPKLKSLSRTSSRSISITESPVSSNEESFEDLTEESITLAWNNIAKLKLDEQYKIHKEAITGIAVTCSGSSVLTTSQDSTLKMFSKESKTIQRSVSFSNMALSSCLILPGDTTVISSSWDNHIYFYSIAFGRQQDILMGHDDAVSKICWRDGRLYSASWDSTVKVWHCVPGEATSNKKHHFDLLAELEHDVSVNTIDLNAANTMLASGTKEGTISVWDVATATVLHQLPCHSGTVFDAAFSPDSRHLLSAGEDCAFKVIDVQTGMLISSVTAAEPQRCFKWNGNTILSGSQSGELLIWDLLGGKVLERIKGHTGAVTSLWMNEQCTSIITGGEDKQIMFWKLQY

Target ORF information:

RefSeq Version	XM_010136596.1
Organism	Buceros rhinoceros silvestris
Definition	Buceros rhinoceros silvestris neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010136596.1

ORF Insert Sequence:

ATGGTTCTCG AGAGGGACCC TGTTGATTCA GTAACTGTCA GAGGCTCTAC TTATTTACGT 
GGAAGAAGTC TGTTCTGTGA AGAGAAAAAG CTCTGGCTGT ACTCTAAGGA GAAGTTTTCA 
CTGCTTCTTC TTAACCTAGA AGAATACTAC TTTGAACAGC ACACAGCTAA TCATATTATA 
AATAAAGGCT GCAGAGATGA AAGAAAATTC AGAGGCTCTC TAAAAATCTG TTCCAAGTCA 
ATCATTTTTG AACCTGATGA CAATAACGAA CCCATTATCA AGATACCCCT GAGAGACTGC 
GTCAGTATAA AAGCCCCGGA AGATAATGAA GCAAATAACC TGTTCACACG GGACACCTCT 
GGAGGGATTT CTGTTGTGTG TAACCAGGCT TTTCTCATTA AAGAAAGGAA CATTATTGCA 
CCCTATAAAA CAGTTAGAGG TAAAACAGAA CACTTGTTCC AGCTGGATGT CGCTGGGAAA 
CTAGGAGACG TTGTGCAAAC TCTGCACCAG CTTTACAGGG CTTCTTGTCT AGACAAGATG 
GGGGATCAAG CTGCCATGAT TACTGCTATA TTGCAGTCGC GCTTGGCTAG AACTTCATTT 
GATAAAAACA GATTTCAAAG CATTTCTGAA ACGCTGCATA TGGAATGTAA AGCAGAAATG 
GTGACACCAC TGGTGACTAA TCCAGGGCAT GTGTGTGTTA CTGATGCTAA CTTGTACTTC 
CAGCCTCTTA ATGGATATCC TAAACCAGTC GTACAAATAA CACTGCAAAA TGTGCGTCGC 
ATCTATAAGA GACGACATGG GCTAATGCCG CTGGGACTAG AAGTATTTTG CACAGAAAAT 
GATTTATGTT CTGACATCTA CTTGAAATTC TACAACTATC AAGATCGAGA TGAGCTCTAT 
TTCCTCATTG CTACGTATAT AGAAAATCAT GTGACAGAGC ATACTGCTGA AAGTTATATG 
TTGCAGTGGC AGCGAGGACA TCTATCAAAT TACCAGTATC TTCTTCATCT CAACAATCTG 
GCTGACAGAA GCTGCAACGA TCTCTCACAG TATCCCGTAT TTCCCTGGAT CATAGCAGAC 
TACTCCAGTT CAGTGTTAGA TCTGACAAAG CCTGAAACAT TCCGTGACCT TAGTAAACCG 
ATTGGTGCCC TGAATAAGGA AAGGCTGGAT AGACTATTGA CACGTTATCA GGAAATGCCA 
GAGCCCAAGT TCATGTATGG GAGCCATTAT TCATCTCCGG GCTATGTGTT GTTTTACCTC 
GTCCGAGTTG CTCCAGAGTA CATGCTCTGT CTGCAGAATG GCAAGTTTGA TCACGCTGAC 
AGAATGTTCA ACAGTCTTGC AGAAACCTGG AAAAACTGTT TGGATGGTGC AACAGATTTC 
AAAGAGTTGA TTCCAGAATT TTATGAAAAT GATTCTAGTT TTCTAGTAAA CAGCTTGAAG 
TTGGACTTGG GAAAAAGGCA GGGTGGAAAG ATGGTTGAAG ATGTTGAGCT TCCCCCTTGG 
GCGTCAGGTC CTGATGACTT TCTTCAGAAG AGCCGAGAGG CTTTAGAAAG TCAGTATGTA 
TCAGAACATC TTCACGAATG GATTGACATA ATATTTGGCT ACAAGCAGAA AGGGAGTGAA 
GCGGTTGCAG CTCAAAACGT GTTTCATCCA TTAACTTACG AAGGAGGAGT GGATTTAAAC 
AGCATTATGG ACCCCAATGA GAAAGTAGCT CTGCTGACAC AGATCTTGGA ATTTGGACAG 
ACGCCAAAGC AGTTGTTTAC AATTCCTCAC CCTCAGAGGA TAATGCCGAA GTTGAAAAGC 
TTGTCTCGAA CATCGAGTCG CAGCATTTCC ATAACTGAGT CTCCAGTTTC TTCAAATGAA 
GAATCTTTTG AAGATTTGAC AGAAGAAAGC ATAACACTTG CTTGGAACAA TATTGCCAAA 
CTAAAATTAG ATGAGCAATA TAAAATTCAC AAAGAGGCCA TTACTGGAAT AGCAGTCACT 
TGCAGTGGGT CTTCTGTTCT CACTACATCC CAGGATTCAA CCTTAAAGAT GTTTTCCAAA 
GAATCAAAAA CAATCCAGAG AAGCGTGTCC TTTTCAAACA TGGCTCTGTC GTCTTGTCTC 
ATCCTACCAG GAGACACCAC TGTCATAAGT TCTTCGTGGG ACAATCATAT ATATTTTTAT 
TCAATTGCAT TTGGAAGGCA GCAGGACATC TTAATGGGGC ATGACGATGC AGTCAGCAAG 
ATCTGCTGGC GTGATGGGCG TTTATATTCC GCATCCTGGG ATTCCACCGT GAAGGTGTGG 
CATTGCGTCC CCGGAGAGGC CACGAGCAAT AAAAAACACC ACTTTGACCT GCTGGCAGAG 
CTGGAACACG ATGTTAGCGT GAATACAATC GACCTTAACG CTGCCAACAC TATGCTGGCA 
TCTGGAACCA AAGAGGGTAC CATCAGCGTT TGGGATGTTG CCACAGCAAC AGTGTTGCAC 
CAGCTGCCCT GTCATTCCGG GACAGTGTTT GACGCTGCTT TCAGTCCTGA CAGCAGACAC 
CTCCTCAGCG CAGGAGAGGA CTGTGCTTTT AAAGTAATAG ATGTTCAGAC TGGAATGCTC 
ATCTCTTCTG TAACGGCTGC GGAGCCACAG AGGTGCTTCA AATGGAATGG AAACACCATC 
TTATCAGGAA GTCAGTCTGG TGAATTACTG ATTTGGGACC TGCTGGGAGG AAAAGTTCTG 
GAAAGAATCA AAGGACATAC AGGTGCTGTA ACATCCCTGT GGATGAACGA ACAGTGCACC 
AGCATTATTA CAGGAGGGGA AGACAAACAG ATCATGTTCT GGAAGCTGCA GTACTAA