VPS18 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS18
Entrez Gene ID	102409323
Full Name	VPS18, CORVET/HOPS core subunit
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006066251.1	XP_006066313.1	vacuolar protein sorting-associated protein 18 homolog
XM_006066251.2	XP_006066313.1	vacuolar protein sorting-associated protein 18 homolog

Pathways from BioSystems
Gene Ontology

The following VPS18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu21633	XM_006066251.1 Latest version!	Bubalus bubalis vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OBu45050	XM_006066251.2 Latest version!	Bubalus bubalis VPS18, CORVET/HOPS core subunit (VPS18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS18 ( XM_006066251.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066313.1 Bubalus bubalis vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18), mRNA.

Price & Availability↑

CloneID	OBu21633
Clone ID Related Accession (Same CDS sequence)	XM_006066251.1
Accession Version	XM_006066251.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	vacuolar protein sorting-associated protein 18 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785199.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTA TCCTGGATGA ATACGAGGAC TCCCTGTCCC GCTCGACCGT CTTGCAGCCC 
GGCTGCCCCA GCGTGGGCAT CCCCCACTCG GGATATGTGA ATGCCCAGCT AGAGAAGGAA 
GCGCCCATCT TCACGAAGCA GCGCATTGAC TTTACCCCTT CCGAGCGCAT CACCAATCTT 
GTCGTCTCCT GCAATCAGCT CTGCATGAGC CTTGGCAAGG ATACACTACT CCGCATTGAC 
TTGGGCAAAG CAAATGAACC CAACCACATG GAGCTGGGGC GCAAGGATGA TGCCAAAGTT 
CACAAGATGT TCCTGGACCC CACTGGCTCT CATCTGCTGA TCGCTCTGAG CAGCACTGAG 
GTCCTCTATG TGAATCGTAA TGGACAGAAG GTTCGGCCCC TGGCACGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGCTG GAATAAGGCC CTGGGCACTG AGAGCAGCAC GGGCCCCATC 
CTGGTTGGCA CCGCCCAAGG GCAGATCTTC GAAGCAGAGC TTTCAGCCAG CGAGGGTGGG 
CTTTTTGGCC CTGCCCCGGA TCTCTACTTC CGTCCATTGT ACGTGCTAAA TGAAGAAGGG 
GGCCCAGCAC CTGTGTGCTC CCTTGAGGCT GAGCGGGGCC CTGAAGGGCG CGGTTTTGTC 
ATCGCCACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGTGGCGGA GGGAGCTGAG 
GCCCAGGGTT TCTCGGGGCT CTTTGCTGCC TACGCTGACC ACCCACCCCC ATTCCGTGAG 
TTCCCCAGCA GTCTGGGCTA CAGCGAGCTG GCCTTCTACA CCCCCAAGTT GCGCTCTGCG 
CCCCGGGCCT TCGCCTGGAT GATGGGAGAT GGCGTGTTGT ATGGATCATT GGACTGCGGG 
CGTCCGGACT CCCTGCTGAG TGAGGAGCGG GTCTGGGAGT ACCCAGAGGG GGTGGGTCCC 
GGGGCCAGCC CACCCCTGGC CATTGTCCTG ACCCAGTTCC ACTTCCTGCT GCTGCTGGCA 
GACCGGGTGG AGGCAGTGTG CACGCTGACG GGACAGGTGG TGCTGCGGGA CCACTTCCTG 
GAGAAGTTTG GGCCGCTGAA GCACATGGTG AAGGACTCCT CCACGGGCCA CCTGTGGGCC 
CACACCGAGC GGGCTGTCTT CCGCTACCAC GTACAGCGGG AGGCACGGGA TGTCTGGCGC 
ACCTACCTGG ACATGAACCG CTTCGACCTG GCCAAGGAGT ATTGTCGAGA GCGGCCTGAC 
TGCCTGGACA CAGTCCTGGC CCGGGAGGCT GACTTCTGCT TCCGCCAGCG TCGCTACCTG 
GAGAGCGCCC GCTGCTATGC CCTGACTCAG AGCTACTTTG AAGAGATTGC CCTTAAGTTC 
TTGGAGGCCC GACAGGAGGA GGCGCTGGCC GAGTTCCTGC AGCGAAAACT AGCCAGTTTG 
AAGCCTGCCG AACGAACCCA GGCAACCCTG CTGACTGCCT GGCTGACGGA GCTCTACTTG 
AGCCGGCTCG GGGCCCTGCA GGGTGACCCC GACGCCCTGA ACCTCTACCG GGAAACCCGT 
GAGCGCTTCC GCTCCTTCCT CAGCAGCCCT CGCCACAAGG AGTGGCTCTT CGCCAGCCGG 
GCCTCCATCC ACGAGCTGTT GGCCAGCCAC GGGGACACGG AGCACATGGT GTACTTTGCT 
GTGATCATGC AGGACTACGA GCGCGTGGTG GCTTACCACT GCCAGCATGA GGCCTACGAG 
GAGGCCTTGG CCGTGCTGGC CCGCCACCGT GATCCCCAGC TCTTCTACAA GTTCTCGCCC 
ATCCTCATCC GTCACATCCC TCGCCAGCTG GTGGACGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCCC GGCAGCTCAT CCCTGCCCTG GTGAACTATA GCCAAGGTGG CGAGGCCCAG 
CAGGTGAGCC AAGCCATCCG CTACATGGAG TTCTGCGTGA ACGTGCTGGG CGAGACCGAG 
CAGGCCATTC ACAATTACCT GCTGTCGCTC TATGCCCGGG GCCAGCCGGC CTCACTGTTG 
GCCTACCTGG AGCAGGCCGG GGCCAGCCCG CATCGCGTGC ATTATGATCT CAAGTATGCG 
CTGCGGCTCT GCGCTGAGCA CGGCCACCAC CGTGCTTGTG TCCACGTCTA CAAGGTCCTG 
GAGCTGTATG AGGAGGCTGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCTGACC TGCCTGAGGA AGACGAAGAG CTGCGCAAGA AGCTGTGGCT GAAGATTGCG 
CGGCACGTGG TGCAGGAGGA GGAAGATGTG CAGACGGCCA TGGCCTGCCT GGCCAGCTGC 
CCCCTGCTCA AGATTGAAGA TGTGCTGCCT TTCTTTCCTG ACTTCGTCAC CATTGACCAC 
TTCAAGGAGG CCATCTGCAG CTCACTGAAG GCCTACAACC ACCACATCCA AGAGCTACAG 
CGAGAGATGG AAGAGGCCAC AGCCAGTGCC CAGCGCATCC GGAGAGACCT GCAGGAGCTG 
CGGGGCCGCT ATGGCACCGT AGAGCCCCAG GACAAATGTG CCACCTGCGA CTTCCCCCTG 
CTCAACCGCC CCTTTTACCT CTTCCTCTGT GGCCACATGT TCCATGCTGA CTGCTTGCTG 
CAGGCCGTGC GGCCTGGCCT GCCGGCCTAC AAGCAGGCCC GGCTCGAGGA GCTGCAGCGA 
AAGCTGGGGG CTGCTCCACC CCCTGCCAAG GGCTCTGCCC GGGCTAAGGA GGCTGAGGGG 
GGCGCTGCTG CTGGGGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTGGA TGAACTTGTG 
GCCGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATCCGCT CTATTGACCG GCCCTTCATT 
GACCCCCAGC GCTATGAGGA GGAGCACCTC AGTTGGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066313.1
CDS	350..3271
Translation	MASILDEYEDSLSRSTVLQPGCPSVGIPHSGYVNAQLEKEAPIFTKQRIDFTPSERITNLVVSCNQLCMSLGKDTLLRIDLGKANEPNHMELGRKDDAKVHKMFLDPTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGQIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPEGRGFVIATTRQRLFQFIGRVAEGAEAQGFSGLFAAYADHPPPFREFPSSLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGSLDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGHLWAHTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTAWLTELYLSRLGALQGDPDALNLYRETRERFRSFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEAQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGQPASLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAAAGGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEHLSWL

Target ORF information:

RefSeq Version	XM_006066251.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis vacuolar protein sorting 18 homolog (S. cerevisiae) (VPS18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066251.1

ORF Insert Sequence:

ATGGCGTCTA TCCTGGATGA ATACGAGGAC TCCCTGTCCC GCTCGACCGT CTTGCAGCCC 
GGCTGCCCCA GCGTGGGCAT CCCCCACTCG GGATATGTGA ATGCCCAGCT AGAGAAGGAA 
GCGCCCATCT TCACGAAGCA GCGCATTGAC TTTACCCCTT CCGAGCGCAT CACCAATCTT 
GTCGTCTCCT GCAATCAGCT CTGCATGAGC CTTGGCAAGG ATACACTACT CCGCATTGAC 
TTGGGCAAAG CAAATGAACC CAACCACATG GAGCTGGGGC GCAAGGATGA TGCCAAAGTT 
CACAAGATGT TCCTGGACCC CACTGGCTCT CATCTGCTGA TCGCTCTGAG CAGCACTGAG 
GTCCTCTATG TGAATCGTAA TGGACAGAAG GTTCGGCCCC TGGCACGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGCTG GAATAAGGCC CTGGGCACTG AGAGCAGCAC GGGCCCCATC 
CTGGTTGGCA CCGCCCAAGG GCAGATCTTC GAAGCAGAGC TTTCAGCCAG CGAGGGTGGG 
CTTTTTGGCC CTGCCCCGGA TCTCTACTTC CGTCCATTGT ACGTGCTAAA TGAAGAAGGG 
GGCCCAGCAC CTGTGTGCTC CCTTGAGGCT GAGCGGGGCC CTGAAGGGCG CGGTTTTGTC 
ATCGCCACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGTGGCGGA GGGAGCTGAG 
GCCCAGGGTT TCTCGGGGCT CTTTGCTGCC TACGCTGACC ACCCACCCCC ATTCCGTGAG 
TTCCCCAGCA GTCTGGGCTA CAGCGAGCTG GCCTTCTACA CCCCCAAGTT GCGCTCTGCG 
CCCCGGGCCT TCGCCTGGAT GATGGGAGAT GGCGTGTTGT ATGGATCATT GGACTGCGGG 
CGTCCGGACT CCCTGCTGAG TGAGGAGCGG GTCTGGGAGT ACCCAGAGGG GGTGGGTCCC 
GGGGCCAGCC CACCCCTGGC CATTGTCCTG ACCCAGTTCC ACTTCCTGCT GCTGCTGGCA 
GACCGGGTGG AGGCAGTGTG CACGCTGACG GGACAGGTGG TGCTGCGGGA CCACTTCCTG 
GAGAAGTTTG GGCCGCTGAA GCACATGGTG AAGGACTCCT CCACGGGCCA CCTGTGGGCC 
CACACCGAGC GGGCTGTCTT CCGCTACCAC GTACAGCGGG AGGCACGGGA TGTCTGGCGC 
ACCTACCTGG ACATGAACCG CTTCGACCTG GCCAAGGAGT ATTGTCGAGA GCGGCCTGAC 
TGCCTGGACA CAGTCCTGGC CCGGGAGGCT GACTTCTGCT TCCGCCAGCG TCGCTACCTG 
GAGAGCGCCC GCTGCTATGC CCTGACTCAG AGCTACTTTG AAGAGATTGC CCTTAAGTTC 
TTGGAGGCCC GACAGGAGGA GGCGCTGGCC GAGTTCCTGC AGCGAAAACT AGCCAGTTTG 
AAGCCTGCCG AACGAACCCA GGCAACCCTG CTGACTGCCT GGCTGACGGA GCTCTACTTG 
AGCCGGCTCG GGGCCCTGCA GGGTGACCCC GACGCCCTGA ACCTCTACCG GGAAACCCGT 
GAGCGCTTCC GCTCCTTCCT CAGCAGCCCT CGCCACAAGG AGTGGCTCTT CGCCAGCCGG 
GCCTCCATCC ACGAGCTGTT GGCCAGCCAC GGGGACACGG AGCACATGGT GTACTTTGCT 
GTGATCATGC AGGACTACGA GCGCGTGGTG GCTTACCACT GCCAGCATGA GGCCTACGAG 
GAGGCCTTGG CCGTGCTGGC CCGCCACCGT GATCCCCAGC TCTTCTACAA GTTCTCGCCC 
ATCCTCATCC GTCACATCCC TCGCCAGCTG GTGGACGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCCC GGCAGCTCAT CCCTGCCCTG GTGAACTATA GCCAAGGTGG CGAGGCCCAG 
CAGGTGAGCC AAGCCATCCG CTACATGGAG TTCTGCGTGA ACGTGCTGGG CGAGACCGAG 
CAGGCCATTC ACAATTACCT GCTGTCGCTC TATGCCCGGG GCCAGCCGGC CTCACTGTTG 
GCCTACCTGG AGCAGGCCGG GGCCAGCCCG CATCGCGTGC ATTATGATCT CAAGTATGCG 
CTGCGGCTCT GCGCTGAGCA CGGCCACCAC CGTGCTTGTG TCCACGTCTA CAAGGTCCTG 
GAGCTGTATG AGGAGGCTGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCTGACC TGCCTGAGGA AGACGAAGAG CTGCGCAAGA AGCTGTGGCT GAAGATTGCG 
CGGCACGTGG TGCAGGAGGA GGAAGATGTG CAGACGGCCA TGGCCTGCCT GGCCAGCTGC 
CCCCTGCTCA AGATTGAAGA TGTGCTGCCT TTCTTTCCTG ACTTCGTCAC CATTGACCAC 
TTCAAGGAGG CCATCTGCAG CTCACTGAAG GCCTACAACC ACCACATCCA AGAGCTACAG 
CGAGAGATGG AAGAGGCCAC AGCCAGTGCC CAGCGCATCC GGAGAGACCT GCAGGAGCTG 
CGGGGCCGCT ATGGCACCGT AGAGCCCCAG GACAAATGTG CCACCTGCGA CTTCCCCCTG 
CTCAACCGCC CCTTTTACCT CTTCCTCTGT GGCCACATGT TCCATGCTGA CTGCTTGCTG 
CAGGCCGTGC GGCCTGGCCT GCCGGCCTAC AAGCAGGCCC GGCTCGAGGA GCTGCAGCGA 
AAGCTGGGGG CTGCTCCACC CCCTGCCAAG GGCTCTGCCC GGGCTAAGGA GGCTGAGGGG 
GGCGCTGCTG CTGGGGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTGGA TGAACTTGTG 
GCCGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATCCGCT CTATTGACCG GCCCTTCATT 
GACCCCCAGC GCTATGAGGA GGAGCACCTC AGTTGGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS18 ( XM_006066251.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006066313.1 Bubalus bubalis VPS18, CORVET/HOPS core subunit (VPS18), mRNA.

Price & Availability↑

CloneID	OBu45050
Clone ID Related Accession (Same CDS sequence)	XM_006066251.2
Accession Version	XM_006066251.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	vacuolar protein sorting-associated protein 18 homolog
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037555.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006066251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCTA TCCTGGATGA ATACGAGGAC TCCCTGTCCC GCTCGACCGT CTTGCAGCCC 
GGCTGCCCCA GCGTGGGCAT CCCCCACTCG GGATATGTGA ATGCCCAGCT AGAGAAGGAA 
GCGCCCATCT TCACGAAGCA GCGCATTGAC TTTACCCCTT CCGAGCGCAT CACCAATCTT 
GTCGTCTCCT GCAATCAGCT CTGCATGAGC CTTGGCAAGG ATACACTACT CCGCATTGAC 
TTGGGCAAAG CAAATGAACC CAACCACATG GAGCTGGGGC GCAAGGATGA TGCCAAAGTT 
CACAAGATGT TCCTGGACCC CACTGGCTCT CATCTGCTGA TCGCTCTGAG CAGCACTGAG 
GTCCTCTATG TGAATCGTAA TGGACAGAAG GTTCGGCCCC TGGCACGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGCTG GAATAAGGCC CTGGGCACTG AGAGCAGCAC GGGCCCCATC 
CTGGTTGGCA CCGCCCAAGG GCAGATCTTC GAAGCAGAGC TTTCAGCCAG CGAGGGTGGG 
CTTTTTGGCC CTGCCCCGGA TCTCTACTTC CGTCCATTGT ACGTGCTAAA TGAAGAAGGG 
GGCCCAGCAC CTGTGTGCTC CCTTGAGGCT GAGCGGGGCC CTGAAGGGCG CGGTTTTGTC 
ATCGCCACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGTGGCGGA GGGAGCTGAG 
GCCCAGGGTT TCTCGGGGCT CTTTGCTGCC TACGCTGACC ACCCACCCCC ATTCCGTGAG 
TTCCCCAGCA GTCTGGGCTA CAGCGAGCTG GCCTTCTACA CCCCCAAGTT GCGCTCTGCG 
CCCCGGGCCT TCGCCTGGAT GATGGGAGAT GGCGTGTTGT ATGGATCATT GGACTGCGGG 
CGTCCGGACT CCCTGCTGAG TGAGGAGCGG GTCTGGGAGT ACCCAGAGGG GGTGGGTCCC 
GGGGCCAGCC CACCCCTGGC CATTGTCCTG ACCCAGTTCC ACTTCCTGCT GCTGCTGGCA 
GACCGGGTGG AGGCAGTGTG CACGCTGACG GGACAGGTGG TGCTGCGGGA CCACTTCCTG 
GAGAAGTTTG GGCCGCTGAA GCACATGGTG AAGGACTCCT CCACGGGCCA CCTGTGGGCC 
CACACCGAGC GGGCTGTCTT CCGCTACCAC GTACAGCGGG AGGCACGGGA TGTCTGGCGC 
ACCTACCTGG ACATGAACCG CTTCGACCTG GCCAAGGAGT ATTGTCGAGA GCGGCCTGAC 
TGCCTGGACA CAGTCCTGGC CCGGGAGGCT GACTTCTGCT TCCGCCAGCG TCGCTACCTG 
GAGAGCGCCC GCTGCTATGC CCTGACTCAG AGCTACTTTG AAGAGATTGC CCTTAAGTTC 
TTGGAGGCCC GACAGGAGGA GGCGCTGGCC GAGTTCCTGC AGCGAAAACT AGCCAGTTTG 
AAGCCTGCCG AACGAACCCA GGCAACCCTG CTGACTGCCT GGCTGACGGA GCTCTACTTG 
AGCCGGCTCG GGGCCCTGCA GGGTGACCCC GACGCCCTGA ACCTCTACCG GGAAACCCGT 
GAGCGCTTCC GCTCCTTCCT CAGCAGCCCT CGCCACAAGG AGTGGCTCTT CGCCAGCCGG 
GCCTCCATCC ACGAGCTGTT GGCCAGCCAC GGGGACACGG AGCACATGGT GTACTTTGCT 
GTGATCATGC AGGACTACGA GCGCGTGGTG GCTTACCACT GCCAGCATGA GGCCTACGAG 
GAGGCCTTGG CCGTGCTGGC CCGCCACCGT GATCCCCAGC TCTTCTACAA GTTCTCGCCC 
ATCCTCATCC GTCACATCCC TCGCCAGCTG GTGGACGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCCC GGCAGCTCAT CCCTGCCCTG GTGAACTATA GCCAAGGTGG CGAGGCCCAG 
CAGGTGAGCC AAGCCATCCG CTACATGGAG TTCTGCGTGA ACGTGCTGGG CGAGACCGAG 
CAGGCCATTC ACAATTACCT GCTGTCGCTC TATGCCCGGG GCCAGCCGGC CTCACTGTTG 
GCCTACCTGG AGCAGGCCGG GGCCAGCCCG CATCGCGTGC ATTATGATCT CAAGTATGCG 
CTGCGGCTCT GCGCTGAGCA CGGCCACCAC CGTGCTTGTG TCCACGTCTA CAAGGTCCTG 
GAGCTGTATG AGGAGGCTGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCTGACC TGCCTGAGGA AGACGAAGAG CTGCGCAAGA AGCTGTGGCT GAAGATTGCG 
CGGCACGTGG TGCAGGAGGA GGAAGATGTG CAGACGGCCA TGGCCTGCCT GGCCAGCTGC 
CCCCTGCTCA AGATTGAAGA TGTGCTGCCT TTCTTTCCTG ACTTCGTCAC CATTGACCAC 
TTCAAGGAGG CCATCTGCAG CTCACTGAAG GCCTACAACC ACCACATCCA AGAGCTACAG 
CGAGAGATGG AGGAGGCCAC AGCCAGTGCC CAGCGCATCC GGAGAGACCT GCAGGAGCTG 
CGGGGCCGCT ATGGCACCGT AGAGCCCCAG GACAAATGTG CCACCTGCGA CTTCCCCCTG 
CTCAACCGCC CCTTTTACCT CTTCCTCTGT GGCCACATGT TCCATGCTGA CTGCTTGCTG 
CAGGCCGTGC GGCCTGGCCT GCCGGCCTAC AAGCAGGCCC GGCTCGAGGA GCTGCAGCGA 
AAGCTGGGGG CTGCTCCACC CCCTGCCAAG GGCTCTGCCC GGGCTAAGGA GGCTGAGGGG 
GGCGCTGCTG CTGGGGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTGGA TGAACTTGTG 
GCCGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATCCGCT CTATTGACCG GCCCTTCATT 
GACCCCCAGC GCTATGAGGA GGAGCACCTC AGTTGGTTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006066313.1
CDS	370..3291
Translation	MASILDEYEDSLSRSTVLQPGCPSVGIPHSGYVNAQLEKEAPIFTKQRIDFTPSERITNLVVSCNQLCMSLGKDTLLRIDLGKANEPNHMELGRKDDAKVHKMFLDPTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGQIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPEGRGFVIATTRQRLFQFIGRVAEGAEAQGFSGLFAAYADHPPPFREFPSSLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGSLDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGHLWAHTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTAWLTELYLSRLGALQGDPDALNLYRETRERFRSFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEAQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGQPASLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAAAGGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEHLSWL

Target ORF information:

RefSeq Version	XM_006066251.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis VPS18, CORVET/HOPS core subunit (VPS18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006066251.2

ORF Insert Sequence:

ATGGCGTCTA TCCTGGATGA ATACGAGGAC TCCCTGTCCC GCTCGACCGT CTTGCAGCCC 
GGCTGCCCCA GCGTGGGCAT CCCCCACTCG GGATATGTGA ATGCCCAGCT AGAGAAGGAA 
GCGCCCATCT TCACGAAGCA GCGCATTGAC TTTACCCCTT CCGAGCGCAT CACCAATCTT 
GTCGTCTCCT GCAATCAGCT CTGCATGAGC CTTGGCAAGG ATACACTACT CCGCATTGAC 
TTGGGCAAAG CAAATGAACC CAACCACATG GAGCTGGGGC GCAAGGATGA TGCCAAAGTT 
CACAAGATGT TCCTGGACCC CACTGGCTCT CATCTGCTGA TCGCTCTGAG CAGCACTGAG 
GTCCTCTATG TGAATCGTAA TGGACAGAAG GTTCGGCCCC TGGCACGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGCTG GAATAAGGCC CTGGGCACTG AGAGCAGCAC GGGCCCCATC 
CTGGTTGGCA CCGCCCAAGG GCAGATCTTC GAAGCAGAGC TTTCAGCCAG CGAGGGTGGG 
CTTTTTGGCC CTGCCCCGGA TCTCTACTTC CGTCCATTGT ACGTGCTAAA TGAAGAAGGG 
GGCCCAGCAC CTGTGTGCTC CCTTGAGGCT GAGCGGGGCC CTGAAGGGCG CGGTTTTGTC 
ATCGCCACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGTGGCGGA GGGAGCTGAG 
GCCCAGGGTT TCTCGGGGCT CTTTGCTGCC TACGCTGACC ACCCACCCCC ATTCCGTGAG 
TTCCCCAGCA GTCTGGGCTA CAGCGAGCTG GCCTTCTACA CCCCCAAGTT GCGCTCTGCG 
CCCCGGGCCT TCGCCTGGAT GATGGGAGAT GGCGTGTTGT ATGGATCATT GGACTGCGGG 
CGTCCGGACT CCCTGCTGAG TGAGGAGCGG GTCTGGGAGT ACCCAGAGGG GGTGGGTCCC 
GGGGCCAGCC CACCCCTGGC CATTGTCCTG ACCCAGTTCC ACTTCCTGCT GCTGCTGGCA 
GACCGGGTGG AGGCAGTGTG CACGCTGACG GGACAGGTGG TGCTGCGGGA CCACTTCCTG 
GAGAAGTTTG GGCCGCTGAA GCACATGGTG AAGGACTCCT CCACGGGCCA CCTGTGGGCC 
CACACCGAGC GGGCTGTCTT CCGCTACCAC GTACAGCGGG AGGCACGGGA TGTCTGGCGC 
ACCTACCTGG ACATGAACCG CTTCGACCTG GCCAAGGAGT ATTGTCGAGA GCGGCCTGAC 
TGCCTGGACA CAGTCCTGGC CCGGGAGGCT GACTTCTGCT TCCGCCAGCG TCGCTACCTG 
GAGAGCGCCC GCTGCTATGC CCTGACTCAG AGCTACTTTG AAGAGATTGC CCTTAAGTTC 
TTGGAGGCCC GACAGGAGGA GGCGCTGGCC GAGTTCCTGC AGCGAAAACT AGCCAGTTTG 
AAGCCTGCCG AACGAACCCA GGCAACCCTG CTGACTGCCT GGCTGACGGA GCTCTACTTG 
AGCCGGCTCG GGGCCCTGCA GGGTGACCCC GACGCCCTGA ACCTCTACCG GGAAACCCGT 
GAGCGCTTCC GCTCCTTCCT CAGCAGCCCT CGCCACAAGG AGTGGCTCTT CGCCAGCCGG 
GCCTCCATCC ACGAGCTGTT GGCCAGCCAC GGGGACACGG AGCACATGGT GTACTTTGCT 
GTGATCATGC AGGACTACGA GCGCGTGGTG GCTTACCACT GCCAGCATGA GGCCTACGAG 
GAGGCCTTGG CCGTGCTGGC CCGCCACCGT GATCCCCAGC TCTTCTACAA GTTCTCGCCC 
ATCCTCATCC GTCACATCCC TCGCCAGCTG GTGGACGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCCC GGCAGCTCAT CCCTGCCCTG GTGAACTATA GCCAAGGTGG CGAGGCCCAG 
CAGGTGAGCC AAGCCATCCG CTACATGGAG TTCTGCGTGA ACGTGCTGGG CGAGACCGAG 
CAGGCCATTC ACAATTACCT GCTGTCGCTC TATGCCCGGG GCCAGCCGGC CTCACTGTTG 
GCCTACCTGG AGCAGGCCGG GGCCAGCCCG CATCGCGTGC ATTATGATCT CAAGTATGCG 
CTGCGGCTCT GCGCTGAGCA CGGCCACCAC CGTGCTTGTG TCCACGTCTA CAAGGTCCTG 
GAGCTGTATG AGGAGGCTGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCTGACC TGCCTGAGGA AGACGAAGAG CTGCGCAAGA AGCTGTGGCT GAAGATTGCG 
CGGCACGTGG TGCAGGAGGA GGAAGATGTG CAGACGGCCA TGGCCTGCCT GGCCAGCTGC 
CCCCTGCTCA AGATTGAAGA TGTGCTGCCT TTCTTTCCTG ACTTCGTCAC CATTGACCAC 
TTCAAGGAGG CCATCTGCAG CTCACTGAAG GCCTACAACC ACCACATCCA AGAGCTACAG 
CGAGAGATGG AGGAGGCCAC AGCCAGTGCC CAGCGCATCC GGAGAGACCT GCAGGAGCTG 
CGGGGCCGCT ATGGCACCGT AGAGCCCCAG GACAAATGTG CCACCTGCGA CTTCCCCCTG 
CTCAACCGCC CCTTTTACCT CTTCCTCTGT GGCCACATGT TCCATGCTGA CTGCTTGCTG 
CAGGCCGTGC GGCCTGGCCT GCCGGCCTAC AAGCAGGCCC GGCTCGAGGA GCTGCAGCGA 
AAGCTGGGGG CTGCTCCACC CCCTGCCAAG GGCTCTGCCC GGGCTAAGGA GGCTGAGGGG 
GGCGCTGCTG CTGGGGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTGGA TGAACTTGTG 
GCCGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATCCGCT CTATTGACCG GCCCTTCATT 
GACCCCCAGC GCTATGAGGA GGAGCACCTC AGTTGGTTGT AG