VCL cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	VCL
Entrez Gene ID	102406026
Full Name	vinculin
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006052639.2	XP_006052701.1	vinculin isoform X3
XM_006052640.2	XP_006052702.1	vinculin isoform X4
XM_006052637.2	XP_006052699.1	vinculin isoform X1
XM_006052637.1	XP_006052699.1	vinculin isoform X1
XM_006052639.1	XP_006052701.1	vinculin isoform X3
XM_006052640.1	XP_006052702.1	vinculin isoform X4
XM_006052638.1	XP_006052700.1	vinculin isoform X2
XM_006052638.2	XP_006052700.1	vinculin isoform X2

Pathways from BioSystems
Gene Ontology

The following VCL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VCL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu10330	XM_006052637.1 Latest version!	Bubalus bubalis vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu10331	XM_006052638.1 Latest version!	Bubalus bubalis vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu10332	XM_006052639.1 Latest version!	Bubalus bubalis vinculin (VCL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu10333	XM_006052640.1 Latest version!	Bubalus bubalis vinculin (VCL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu65393	XM_006052637.2 Latest version!	Bubalus bubalis vinculin (VCL), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu65394	XM_006052638.2 Latest version!	Bubalus bubalis vinculin (VCL), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu65395	XM_006052639.2 Latest version!	Bubalus bubalis vinculin (VCL), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu65396	XM_006052640.2 Latest version!	Bubalus bubalis vinculin (VCL), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VCL ( XM_006052637.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052699.1 Bubalus bubalis vinculin (VCL), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu10330
Clone ID Related Accession (Same CDS sequence)	XM_006052637.1
Accession Version	XM_006052637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784481.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGACGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGAA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTGGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCTCGA GAGCTCACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCG CGCAAGTGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAAG TGTTGTAGCT GAGGCAGAGG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GACTGGTCAG AGGGGGCAGC GGCACCAAGC GGGCACTGAT TCAGTGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGGATTAG AACCAACCTG TTACAGGTAT GCGAGCGAAT CCCAACCATA 
AGCACCCAGC TCAAAATCCT GTCCACGGTG AAGGCCACCA TGCTGGGCCG GACCAACATA 
AGTGACGAGG AGTCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACAGAT 
GCTGGGTTTA CTCTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052699.1
CDS	118..3525
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGISVVAEAEAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006052637.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052637.1

ORF Insert Sequence:

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGACGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGAA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTGGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCTCGA GAGCTCACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCG CGCAAGTGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAAG TGTTGTAGCT GAGGCAGAGG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GACTGGTCAG AGGGGGCAGC GGCACCAAGC GGGCACTGAT TCAGTGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGGATTAG AACCAACCTG TTACAGGTAT GCGAGCGAAT CCCAACCATA 
AGCACCCAGC TCAAAATCCT GTCCACGGTG AAGGCCACCA TGCTGGGCCG GACCAACATA 
AGTGACGAGG AGTCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACAGAT 
GCTGGGTTTA CTCTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_006052638.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052700.1 Bubalus bubalis vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu10331
Clone ID Related Accession (Same CDS sequence)	XM_006052638.1
Accession Version	XM_006052638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784481.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GACGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGAAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCTCGAGAG CTCACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCGCGC AAGTGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAAGTGT TGTAGCTGAG GCAGAGGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGAC TGGTCAGAGG GGGCAGCGGC ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTGTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACGGTGAAG GCCACCATGC TGGGCCGGAC CAACATAAGT 
GACGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACAGATGCT 
GGGTTTACTC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052700.1
CDS	117..3521
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGISVVAEAEAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006052638.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052638.1

ORF Insert Sequence:

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GACGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGAAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCTCGAGAG CTCACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCGCGC AAGTGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAAGTGT TGTAGCTGAG GCAGAGGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGAC TGGTCAGAGG GGGCAGCGGC ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTGTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACGGTGAAG GCCACCATGC TGGGCCGGAC CAACATAAGT 
GACGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACAGATGCT 
GGGTTTACTC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_006052639.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052701.1 Bubalus bubalis vinculin (VCL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu10332
Clone ID Related Accession (Same CDS sequence)	XM_006052639.1
Accession Version	XM_006052639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	vinculin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784481.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGACGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGAA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTGGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCTCGA GAGCTCACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCG CGCAAGTGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGACT GGTCAGAGGG 
GGCAGCGGCA CCAAGCGGGC ACTGATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG GATTAGAACC 
AACCTGTTAC AGGTATGCGA GCGAATCCCA ACCATAAGCA CCCAGCTCAA AATCCTGTCC 
ACGGTGAAGG CCACCATGCT GGGCCGGACC AACATAAGTG ACGAGGAGTC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACAGATGCTG GGTTTACTCT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052701.1
CDS	117..3320
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006052639.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis vinculin (VCL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052639.1

ORF Insert Sequence:

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGACGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGAA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTGGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCTCGA GAGCTCACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCG CGCAAGTGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGACT GGTCAGAGGG 
GGCAGCGGCA CCAAGCGGGC ACTGATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG GATTAGAACC 
AACCTGTTAC AGGTATGCGA GCGAATCCCA ACCATAAGCA CCCAGCTCAA AATCCTGTCC 
ACGGTGAAGG CCACCATGCT GGGCCGGACC AACATAAGTG ACGAGGAGTC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACAGATGCTG GGTTTACTCT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_006052640.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052702.1 Bubalus bubalis vinculin (VCL), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu10333
Clone ID Related Accession (Same CDS sequence)	XM_006052640.1
Accession Version	XM_006052640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	vinculin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784481.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GACGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGAAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCTCGAGAG CTCACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCGCGC AAGTGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGACTGGT CAGAGGGGGC 
AGCGGCACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTGTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACG 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATAAGTGACG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGGT TTACTCTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052702.1
CDS	116..3316
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006052640.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis vinculin (VCL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052640.1

ORF Insert Sequence:

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ACGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GACGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGAAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCTCGAGAG CTCACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCGCGC AAGTGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGACTGGT CAGAGGGGGC 
AGCGGCACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTGTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACG 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATAAGTGACG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGGT TTACTCTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_006052637.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052699.1 Bubalus bubalis vinculin (VCL), transcript variant X1, mRNA.

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CloneID	OBu65393
Clone ID Related Accession (Same CDS sequence)	XM_006052637.2
Accession Version	XM_006052637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGACGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGAA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTGGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCTCGA GAGCTCACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCG CGCAAGTGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAAG TGTTGTAGCT GAGGCAGAGG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GACTGGTCAG AGGGGGCAGC GGCACCAAGC GGGCACTGAT TCAGTGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGGATTAG AACCAACCTG TTACAGGTAT GCGAGCGAAT CCCAACCATA 
AGCACCCAGC TCAAAATCCT GTCCACGGTG AAGGCCACCA TGCTGGGCCG GACCAACATA 
AGTGACGAGG AGTCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACAGAT 
GCTGGGTTTA CTCTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052699.1
CDS	113..3520
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGISVVAEAEAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006052637.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis vinculin (VCL), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052637.2

ORF Insert Sequence:

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGACGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGAA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTGGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCTCGA GAGCTCACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCG CGCAAGTGGT CCAGCAAGCC GGGTAACCCA 
GCCGCTAAGG TGGGTATAAG TGTTGTAGCT GAGGCAGAGG CGGCTGATGC TGTTGGGTTC 
CCTGTCCCCC CTGACATGGA AGACGATTAC GAACCTGAGC TGCTGTTAAT GCCATCCAAT 
CAGCCGGTCA ACCAGCCCAT TCTGGCCGCG GCTCAGTCAT TGCATCGGGA AGCTACCAAG 
TGGTCTAGTA AGGGCAATGA CATCATTGCA GCAGCCAAGC GCATGGCTCT GTTGATGGCT 
GAGATGTCTC GACTGGTCAG AGGGGGCAGC GGCACCAAGC GGGCACTGAT TCAGTGTGCC 
AAGGACATCG CCAAGGCCTC AGATGAGGTG ACTCGGTTGG CCAAGGAGGT TGCCAAGCAG 
TGCACAGATA AGCGGATTAG AACCAACCTG TTACAGGTAT GCGAGCGAAT CCCAACCATA 
AGCACCCAGC TCAAAATCCT GTCCACGGTG AAGGCCACCA TGCTGGGCCG GACCAACATA 
AGTGACGAGG AGTCTGAGCA GGCCACAGAG ATGCTGGTTC ACAATGCCCA GAACCTCATG 
CAGTCTGTGA AGGAGACTGT TCGAGAAGCA GAAGCAGCTT CAATCAAAAT TCGAACAGAT 
GCTGGGTTTA CTCTGCGCTG GGTTAGAAAG ACTCCCTGGT ACCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_006052638.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052700.1 Bubalus bubalis vinculin (VCL), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu65394
Clone ID Related Accession (Same CDS sequence)	XM_006052638.2
Accession Version	XM_006052638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	vinculin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GACGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGAAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCTCGAGAG CTCACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCGCGC AAGTGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAAGTGT TGTAGCTGAG GCAGAGGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGAC TGGTCAGAGG GGGCAGCGGC ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTGTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACGGTGAAG GCCACCATGC TGGGCCGGAC CAACATAAGT 
GACGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACAGATGCT 
GGGTTTACTC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052700.1
CDS	113..3517
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGNPAAKVGISVVAEAEAADAVGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006052638.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis vinculin (VCL), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052638.2

ORF Insert Sequence:

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GACGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGAAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCTCGAGAG CTCACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCGCGC AAGTGGTCCA GCAAGCCGGG TAACCCAGCC 
GCTAAGGTGG GTATAAGTGT TGTAGCTGAG GCAGAGGCGG CTGATGCTGT TGGGTTCCCT 
GTCCCCCCTG ACATGGAAGA CGATTACGAA CCTGAGCTGC TGTTAATGCC ATCCAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCATTGC ATCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATTGCAGCA GCCAAGCGCA TGGCTCTGTT GATGGCTGAG 
ATGTCTCGAC TGGTCAGAGG GGGCAGCGGC ACCAAGCGGG CACTGATTCA GTGTGCCAAG 
GACATCGCCA AGGCCTCAGA TGAGGTGACT CGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAACCTGTTA CAGGTATGCG AGCGAATCCC AACCATAAGC 
ACCCAGCTCA AAATCCTGTC CACGGTGAAG GCCACCATGC TGGGCCGGAC CAACATAAGT 
GACGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCACA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAGG AGACTGTTCG AGAAGCAGAA GCAGCTTCAA TCAAAATTCG AACAGATGCT 
GGGTTTACTC TGCGCTGGGT TAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_006052639.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052701.1 Bubalus bubalis vinculin (VCL), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu65395
Clone ID Related Accession (Same CDS sequence)	XM_006052639.2
Accession Version	XM_006052639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3204bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	vinculin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGACGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGAA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTGGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCTCGA GAGCTCACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCG CGCAAGTGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGACT GGTCAGAGGG 
GGCAGCGGCA CCAAGCGGGC ACTGATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG GATTAGAACC 
AACCTGTTAC AGGTATGCGA GCGAATCCCA ACCATAAGCA CCCAGCTCAA AATCCTGTCC 
ACGGTGAAGG CCACCATGCT GGGCCGGACC AACATAAGTG ACGAGGAGTC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACAGATGCTG GGTTTACTCT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052701.1
CDS	112..3315
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006052639.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis vinculin (VCL), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052639.2

ORF Insert Sequence:

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGAAGGACA CTGAAGCCAT GAAGAGAGCA TTGGCCTCCA TAGACTCCAA ACTGAACCAG 
GCTAAAGGTT GGCTCCGTGA TCCCAATGCC TCCCCAGGTG ATGCTGGTGA ACAGGCCATC 
AGGCAGATCT TAGATGAAGC TGGAAAAGTT GGTGAACTCT GTGCAGGCAA AGAACGCAGG 
GAGATCCTGG GAACCTGCAA AATGCTGGGG CAGATGACTG ACCAAGTGGC TGACCTCCGA 
GCCAGAGGAC AAGGAGCCTC ACCGGTGGCC ATGCAGAAAG CCCAGCAAGT GTCTCAGGGT 
CTGGACGTGC TCACAGCAAA AGTGGAAAAT GCAGCCCGCA AGCTGGAAGC CATGACCAAC 
TCAAAGCAGA GCATTGCAAA GAAGATCGAT GCTGCTCAGA ATTGGCTTGC AGATCCAAAT 
GGTGGACCAG AAGGAGAAGA ACAGATTCGA GGGGCTTTGG CTGAAGCTCG GAAAATAGCA 
GAATTATGTG ATGATCCTAA AGAGAGAGAT GACATTCTGC GTTCTCTTGG AGAAATATCT 
GCTCTGACTT CTAAATTAGG AGATCTACGA AGACAAGGAA AAGGAGATTC CCCAGAGGCC 
CGAGCATTAG CCAAACAGGT GGCCACAGCC CTACAGAACT TACAGACTAA AACAAACAGG 
GCCGTGGCCA ACAGCAGACC TGCCAAAGCA GCGGTCCACC TTGAAGGCAA GATTGAGCAA 
GCGCAGCGGT GGATTGATAA TCCTACTGTG GATGACCGGG GAGTTGGTCA GGCTGCCATC 
CGAGGGCTTG TGGCTGAAGG GCATCGTCTG GCTAATGTCA TGATGGGGCC TTATCGCCAG 
GATCTTCTTG CAAAGTGTGA CCGAGTGGAC CAGCTGACAG CCCAGCTGGC TGACCTGGCT 
GCCAGAGGGG AAGGGGAGAG CCCTCAGGCG AGAGCTCTTG CATCTCAGCT CCAAGACTCC 
TTAAAGGATC TGAAAGCCCG GATGCAGGAG GCCATGACTC AGGAGGTGTC AGATGTTTTC 
AGTGATACCA CGACTCCCAT CAAGCTGTTG GCAGTGGCAG CCACTGCTCC TCCTGATGCA 
CCCAATAGGG AAGAGGTGTT TGATGAGAGG GCAGCTAACT TTGAAAACCA TTCAGGAAGG 
CTTGGGGCCA CAGCAGAGAA GGCGGCTGCT GTTGGAACTG CTAATAAATC AACAGTGGAA 
GGCATTCAGG CCTCAGTGAA GACAGCTCGA GAGCTCACAC CCCAGGTGGT CTCAGCTGCT 
CGCATCTTAC TTAGGAATCC TGGAAATCAA GCTGCTTATG AACATTTTGA GACCATGAAG 
AACCAGTGGA TCGATAATGT TGAAAAAATG ACAGGGCTGG TGGACGAAGC CATTGACACT 
AAATCTCTGT TGGATGCTTC CGAAGAAGCA ATTAAAAAAG ACCTGGACAA GTGTAAAGTT 
GCCATGGCCA ACATTCAGCC TCAGATGCTG GTCGCCGGGG CAACCAGCAT TGCTCGTCGG 
GCCAACCGGA TCCTGCTGGT GGCTAAGAGG GAGGTGGAGA ACTCTGAGGA TCCCAAATTC 
CGTGAGGCTG TGAAAGCTGC CTCTGATGAA TTGAGCAAAA CCATCTCCCC AATGGTGGTG 
GATGCAAAGG CCGTGGCAGG AAACATTTCT GACCCTGGAC TGCAAAAGAG CTTCCTGGAC 
TCAGGATACC GGATCCTAGG AGCTGTGGCC AAGGTCAGAG AAGCCTTCCA ACCACAGGAG 
CCTGACTTCC CGCCTCCTCC ACCAGACCTT GAACAGCTCC GACTGACAGA TGAGCTTGCT 
CCTCCCAAAC CACCTCTGCC TGAGGGTGAG GTCCCTCCAC CCAGGCCCCC ACCACCGGAG 
GAGAAGGATG AAGAATTCCC TGAGCAGAAA GCCGGGGAGG TGATTAACCA GCCAATGATG 
ATGGCTGCCA GGCAGCTCCA CGATGAAGCG CGCAAGTGGT CCAGCAAGGG CAATGACATC 
ATTGCAGCAG CCAAGCGCAT GGCTCTGTTG ATGGCTGAGA TGTCTCGACT GGTCAGAGGG 
GGCAGCGGCA CCAAGCGGGC ACTGATTCAG TGTGCCAAGG ACATCGCCAA GGCCTCAGAT 
GAGGTGACTC GGTTGGCCAA GGAGGTTGCC AAGCAGTGCA CAGATAAGCG GATTAGAACC 
AACCTGTTAC AGGTATGCGA GCGAATCCCA ACCATAAGCA CCCAGCTCAA AATCCTGTCC 
ACGGTGAAGG CCACCATGCT GGGCCGGACC AACATAAGTG ACGAGGAGTC TGAGCAGGCC 
ACAGAGATGC TGGTTCACAA TGCCCAGAAC CTCATGCAGT CTGTGAAGGA GACTGTTCGA 
GAAGCAGAAG CAGCTTCAAT CAAAATTCGA ACAGATGCTG GGTTTACTCT GCGCTGGGTT 
AGAAAGACTC CCTGGTACCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VCL ( XM_006052640.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052702.1 Bubalus bubalis vinculin (VCL), transcript variant X4, mRNA.

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CloneID	OBu65396
Clone ID Related Accession (Same CDS sequence)	XM_006052640.2
Accession Version	XM_006052640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	vinculin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037548.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GACGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGAAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCTCGAGAG CTCACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCGCGC AAGTGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGACTGGT CAGAGGGGGC 
AGCGGCACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTGTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACG 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATAAGTGACG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGGT TTACTCTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052702.1
CDS	112..3312
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEISALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKARMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGTANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVVDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006052640.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis vinculin (VCL), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052640.2

ORF Insert Sequence:

ATGCCGGTGT TCCATACGCG CACGATTGAG AGCATTCTGG AGCCGGTGGC GCAGCAGATC 
TCGCACCTGG TGATTATGCA CGAGGAAGGC GAGGTGGACG GCAAAGCCAT CCCTGACCTC 
ACCGCGCCTG TGGCCGCCGT GCAGGCGGCG GTCAGCAACC TCGTTCGGGT TGGAAAAGAG 
ACTGTTCAAA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCACCAGC ATTTATTAAG 
GTGGAGAACG CTTGCACCAA GCTTGTCCAA GCAGCCCAGA TGCTGCAGTC AGACCCTTAC 
TCAGTGCCTG CTCGAGATTA TCTAATTGAT GGGTCAAGGG GTATCCTCTC CGGCACATCA 
GACCTGCTCC TCACCTTCGA CGAGGCTGAG GTTCGTAAAA TTATTAGAGT TTGCAAAGGA 
ATTTTGGAAT ATCTTACAGT GGCAGAGGTG GTAGAAACTA TGGAAGATTT GGTCACTTAC 
ACGAAGAATC TTGGACCAGG AATGACTAAG ATGGCCAAAA TGATTGACGA GAGGCAGCAG 
GAACTGACTC ATCAGGAGCA TCGAGTGATG TTGGTGAACT CAATGAACAC TGTGAAAGAG 
TTGCTGCCAG TTCTCATTTC AGCTATGAAG ATTTTTGTAA CAACTAAAAA CTCCAAAAAC 
CAAGGAATTG AAGAAGCTTT GAAAAATCGC AATTTTACTG TAGAAAAGAT GAGCGCTGAA 
ATTAATGAGA TTATTCGTGT ATTACAACTC ACTTCCTGGG ATGAAGATGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCATTG GCCTCCATAG ACTCCAAACT GAACCAGGCT 
AAAGGTTGGC TCCGTGATCC CAATGCCTCC CCAGGTGATG CTGGTGAACA GGCCATCAGG 
CAGATCTTAG ATGAAGCTGG AAAAGTTGGT GAACTCTGTG CAGGCAAAGA ACGCAGGGAG 
ATCCTGGGAA CCTGCAAAAT GCTGGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCACC GGTGGCCATG CAGAAAGCCC AGCAAGTGTC TCAGGGTCTG 
GACGTGCTCA CAGCAAAAGT GGAAAATGCA GCCCGCAAGC TGGAAGCCAT GACCAACTCA 
AAGCAGAGCA TTGCAAAGAA GATCGATGCT GCTCAGAATT GGCTTGCAGA TCCAAATGGT 
GGACCAGAAG GAGAAGAACA GATTCGAGGG GCTTTGGCTG AAGCTCGGAA AATAGCAGAA 
TTATGTGATG ATCCTAAAGA GAGAGATGAC ATTCTGCGTT CTCTTGGAGA AATATCTGCT 
CTGACTTCTA AATTAGGAGA TCTACGAAGA CAAGGAAAAG GAGATTCCCC AGAGGCCCGA 
GCATTAGCCA AACAGGTGGC CACAGCCCTA CAGAACTTAC AGACTAAAAC AAACAGGGCC 
GTGGCCAACA GCAGACCTGC CAAAGCAGCG GTCCACCTTG AAGGCAAGAT TGAGCAAGCG 
CAGCGGTGGA TTGATAATCC TACTGTGGAT GACCGGGGAG TTGGTCAGGC TGCCATCCGA 
GGGCTTGTGG CTGAAGGGCA TCGTCTGGCT AATGTCATGA TGGGGCCTTA TCGCCAGGAT 
CTTCTTGCAA AGTGTGACCG AGTGGACCAG CTGACAGCCC AGCTGGCTGA CCTGGCTGCC 
AGAGGGGAAG GGGAGAGCCC TCAGGCGAGA GCTCTTGCAT CTCAGCTCCA AGACTCCTTA 
AAGGATCTGA AAGCCCGGAT GCAGGAGGCC ATGACTCAGG AGGTGTCAGA TGTTTTCAGT 
GATACCACGA CTCCCATCAA GCTGTTGGCA GTGGCAGCCA CTGCTCCTCC TGATGCACCC 
AATAGGGAAG AGGTGTTTGA TGAGAGGGCA GCTAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACAG CAGAGAAGGC GGCTGCTGTT GGAACTGCTA ATAAATCAAC AGTGGAAGGC 
ATTCAGGCCT CAGTGAAGAC AGCTCGAGAG CTCACACCCC AGGTGGTCTC AGCTGCTCGC 
ATCTTACTTA GGAATCCTGG AAATCAAGCT GCTTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATCG ATAATGTTGA AAAAATGACA GGGCTGGTGG ACGAAGCCAT TGACACTAAA 
TCTCTGTTGG ATGCTTCCGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAAGTTGCC 
ATGGCCAACA TTCAGCCTCA GATGCTGGTC GCCGGGGCAA CCAGCATTGC TCGTCGGGCC 
AACCGGATCC TGCTGGTGGC TAAGAGGGAG GTGGAGAACT CTGAGGATCC CAAATTCCGT 
GAGGCTGTGA AAGCTGCCTC TGATGAATTG AGCAAAACCA TCTCCCCAAT GGTGGTGGAT 
GCAAAGGCCG TGGCAGGAAA CATTTCTGAC CCTGGACTGC AAAAGAGCTT CCTGGACTCA 
GGATACCGGA TCCTAGGAGC TGTGGCCAAG GTCAGAGAAG CCTTCCAACC ACAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTCCGAC TGACAGATGA GCTTGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAGGTC CCTCCACCCA GGCCCCCACC ACCGGAGGAG 
AAGGATGAAG AATTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC AATGATGATG 
GCTGCCAGGC AGCTCCACGA TGAAGCGCGC AAGTGGTCCA GCAAGGGCAA TGACATCATT 
GCAGCAGCCA AGCGCATGGC TCTGTTGATG GCTGAGATGT CTCGACTGGT CAGAGGGGGC 
AGCGGCACCA AGCGGGCACT GATTCAGTGT GCCAAGGACA TCGCCAAGGC CTCAGATGAG 
GTGACTCGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAC 
CTGTTACAGG TATGCGAGCG AATCCCAACC ATAAGCACCC AGCTCAAAAT CCTGTCCACG 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATAAGTGACG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCACAATGC CCAGAACCTC ATGCAGTCTG TGAAGGAGAC TGTTCGAGAA 
GCAGAAGCAG CTTCAATCAA AATTCGAACA GATGCTGGGT TTACTCTGCG CTGGGTTAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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VCL cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム