VWA8 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	VWA8
Entrez Gene ID	102402132
Full Name	von Willebrand factor A domain containing 8
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025263046.1	XP_025118831.1	LOW QUALITY PROTEIN: von Willebrand factor A domain-containing protein 8
XM_006076533.1	XP_006076595.1	von Willebrand factor A domain-containing protein 8

Pathways from BioSystems
Gene Ontology

The following VWA8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VWA8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu47671	XM_025263046.1 Latest version!	Bubalus bubalis von Willebrand factor A domain containing 8 (VWA8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBu30039	XM_006076533.1 Latest version!	Bubalus bubalis von Willebrand factor A domain containing 8 (VWA8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00

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Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VWA8 ( XM_025263046.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025118831.1 Bubalus bubalis von Willebrand factor A domain containing 8 (VWA8), mRNA.

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CloneID	OBu47671
Clone ID Related Accession (Same CDS sequence)	XM_025263046.1
Accession Version	XM_025263046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: von Willebrand factor A domain-containing protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037557.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGCAGTCGC GGCTCCTGCT CCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCGTG 
CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGAGGC CAGGCTTGTG 
CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG 
TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATCTCAGA CTCTCTGGCT 
CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC 
TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CCTGGAGCTG 
ACCAAAAGGG AGGTTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA 
CAGCGGCGAG AGATCCGTGC TGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA 
GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG 
CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG 
ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT 
TGGAAGATTG TCCGAGTTAG TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC 
AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT 
TATTATCTGC CCTTCAAGGA CCAACTTAAA CTGTTATACT CAGTTGGAGC CAATGTTCCT 
ACTGAAAAAG TTTCTCAGCT CTTATCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT 
ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAT 
TCCTTTCCTA TGATGTCAAT CCAACACGTG CTCCAGCGGC TTTACCCGTA CACTGTGTTA 
CTGGGCCATG AAGGGAGGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGA ACTTGAGGAT 
TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGATGAC CGAAAACAAT 
GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA 
GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC 
CATAAACAAC TACTTGCTGA AATGATTCAG TCTCACATGG TTAAGGACAT ATGCTTAATT 
GGAGGAAAAG GTTGTGGAAA AACAGTCATC ACTAAGAACT TTGCAGATAC CTTGGGATAC 
AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCGC GTGATCTGCT ACAGCAGAGG 
TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG 
GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC GCTTGCCGTG 
TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTGTATG ATGGCTCCAG GCTGCTGAGA 
GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG 
AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT 
ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT 
TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT 
ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACGC ACAAACTCAG AGAGACACAG 
GATCCAACAG CGCAATCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT 
CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT 
TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA 
ATAGAAATAA GCACTGATAA TAGTCCAGAG TCTGAATTGG AGGATTACAA ATGTGAAGTT 
TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA 
ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT 
ATGCTCAAAG ACTTTCTTCT TGGAGAACAC CTATTATTGG TTGGAAACCA GGGTGTAGGG 
AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG 
CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT 
ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA 
GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT AGTAGAAAAT 
GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA 
AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT 
GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT 
GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT 
GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAACTGAG GAATTTAGCT 
GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT 
TTGCAGAAGT TTCCCACTGA AGGTCTGTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT 
TCCTACAACA ACGACATGAG GGAGATTTTG ATTAACACTT TGCATAAATA CGGAATACCA 
GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC 
TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA 
GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA 
ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA 
CCATTGGATG AAATGAACTT AATCTGTGAC GTAGCTACAG CACATGAAAA TGAGGAAAAT 
ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAG 
AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG GATCTGGCAC 
CCATTTGTGA CGGTGGCACC GCTGGGAAAT CCCCTAAAGG GTCAAGTGGT TCTCCATGAG 
CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC 
CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA 
ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA 
GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC 
ATCAACCTCC AGACAGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA 
ACAAATCAGA AGTATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT 
TGCCAGTTGT ATGTGTTGAA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA 
ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT 
TCAGCTGTGG GACAGAAGAT TTCCTCTCCA AACCGACTTC TCTCAGATAA GAATAGTTAT 
GCTACAATTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA 
AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG 
AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACGAA TCAGATAGTC 
AGGATTTTGC CCCCCGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT 
CCACAGACAG CTGGGTATAT AGAAGTTACT GATCTCCAGT CCAAGAGACT TCGATACATC 
CCAATTCCCG GGTCAGACTC TCTATCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC 
ACAGATGCAC TGCTGGCCGA GTGGGGCAAA GGTGGCGTTG TCACTGTTGA TATGGGAGGC 
CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA 
AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA 
GACGTGAGCT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT 
AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACTGCAG GCCTGGGCGG CAAGGGAGGT 
CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTCAGGCCGA GAAAGACGCT 
GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG 
CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT 
GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA 
GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT 
GGGCTAACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAAATGGGC 
AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTGGTAG ACGTGTCGGG CAGCATGTAC 
CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCAATGG AAGCTGTGTG CATGGTCATG 
GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGTATGACA TCGCTGGACA CTCTGGAGAT 
GGATACAACA TTGGTCTAGT TCCAATTAAC AAAATCCCCA AGGACAATAA GCAAAGACTA 
GAAATTCTCA AGACGATGCA CGCCCACGCT CAGTTCTGCA TGAGTGGGGA CCACACCTTA 
GAGGGGACAG AACATGCCAT CAAAGAAATC GTCAAAGAAG AAGCTGATGA GTACTTCGTC 
ATTGTCTTAA GTGATGCCAA CCTGTCTCGC TATGGAATAC ACCCTGCCAA GTTTGCCCAG 
ATCCTGACAA GCAACCCTCA AGTAAATGCT TTCGCCATCT TTATTGGATC TTTAGGCGAT 
CAAGCAGCCA GGCTTCAGAG AACTCTACCA GCGGGCCGGT CTTTCGTTGC CATGGACACC 
AAGGACATCC CTCAGATTTT ACAACAGATT TTCACCTCCA CCATGCTGTC CAGTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025118831.1
CDS	85..5784
Translation	MQSRLLLLGPPGGPPAARRVRLLLRQVVRRQPPHSEARLVHAGAGAGSGDTVNIGDVSYKLKNPKNPELVPQNYISDSLAQSVIQHLRWIMQKDLLGQDVFLIGPPGPLRRSIAMQYLELTKREVEYIALSRDTTETDLKQRREIRAGTAFYIDQCAVRAATEGRTLILEGLEKAERNVLPVLNNLLENREMQLEDGRFLMSAERYDKLLQDHTKTELDAWKIVRVSENFRVIALGLPVPRYSGNPLDPPLRSRFQARDIYYLPFKDQLKLLYSVGANVPTEKVSQLLSFATTLCSQESSTLGLPDFPLDSLPAAVQILDSFPMMSIQHVLQRLYPYTVLLGHEGRMAVQGVLKRFELEDSRRSVLPEKIVRVERMTENNVFQASVTIRIAEKEVTIKVPAGTRPLSQPCTSDRFIQTLSHKQLLAEMIQSHMVKDICLIGGKGCGKTVITKNFADTLGYNIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSPLVNAALEGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYLRLKEELQMSDEQLQQRSIFPVHPSFRIIALAEPPVIGSTTQQWLGPEFLTMFFFHYMKPLVKSEEIQVIKEMVPDMPPEALDKLLSFTHKLRETQDPTAQSLAASLSTRQLLRISRRLSQYPNENLHNAVTKACLSRFLPSLARSALEKNLADAAIEISTDNSPESELEDYKCEVSSGSLRIGTVSAPIYNAHEKMKVPDVLFYDNIQHMIVMEDMLKDFLLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREYIQLHRDTTVQTLTLQPSVKDGLIVYEDSPLVKAVKLGHVLVVDEADKAPTNVTCILKTLVENGEMILADGRRIVANAALVNGRENVVVIHPDFRMIVLANRPGFPFLGNDFFGTLGDIFSCHAVDNPKPYSELRMLRQYGPNVPEPILQKLVAAFGELRNLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFDFDSYNNDMREILINTLHKYGIPVGAKPSNVQLAKELPLPEQTFMGYWTIGQSRNGMQKLLCPAETHHIDVKGPALIHIQEYPIERHEGRSQTFTEECSSWRLPLDEMNLICDVATAHENEENTLYVVACNPVSLYFMNMTGKSGHFVDLFDIFPRTASGIWHPFVTVAPLGNPLKGQVVLHEQQSNVILLLDPASQALRRLILPSEEFTMKKTSWWNKEEAETYKMCKEFSHKNWLVFYKEKGNTLAVLDVLEGRTHTISLPINLQTVFLVAEDKWLLVESKTNQKYLLTTPAHIESKDSGVCQLYVLKEELPSPGFGVMQETDRSIPHTISSDQLSSENLSSAVGQKISSPNRLLSDKNSYATIVVGFPDLMSPSEVYSWKRPSSLSKSRVTDTTFYGGKKKSGTAKQSNCVTLLDTNQIVRILPPGEVPLQDIYPKDVTPPQTAGYIEVTDLQSKRLRYIPIPGSDSLSPYTTWLSTISDTDALLAEWGKGGVVTVDMGGRVRLWETGLEHLQRSLMEWRNMIGEGDRHMQITIERESGEDVSSPKHGKEDPDNMPHVGGNTWAGGTGGRDTAGLGGKGGPYRLDAGHTVYQVSQAEKDAVPEEVKRAAREMGQRAFQQRLKEIQMSEYDAATYERFSGAVRRQVHSLRIILDNLQAKGKERQWLRHQATGELDDAKIIDGLTGEKAIYKRRGELEPQMGSPQQKPKRLRLVVDVSGSMYRFNGVDGRLERSMEAVCMVMEAFENYEEKFKYDIAGHSGDGYNIGLVPINKIPKDNKQRLEILKTMHAHAQFCMSGDHTLEGTEHAIKEIVKEEADEYFVIVLSDANLSRYGIHPAKFAQILTSNPQVNAFAIFIGSLGDQAARLQRTLPAGRSFVAMDTKDIPQILQQIFTSTMLSSV

Target ORF information:

RefSeq Version	XM_025263046.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis von Willebrand factor A domain containing 8 (VWA8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025263046.1

ORF Insert Sequence:

ATGCAGTCGC GGCTCCTGCT CCTCGGGCCG CCTGGCGGCC CTCCGGCCGC GCGGCGCGTG 
CGGCTGCTCC TGCGGCAGGT GGTGCGGCGC CAGCCGCCGC ACTCGGAGGC CAGGCTTGTG 
CACGCCGGGG CGGGGGCCGG CTCAGGTGAT ACAGTTAATA TTGGAGATGT ATCCTACAAG 
TTGAAAAATC CTAAGAACCC AGAACTTGTG CCACAAAACT ACATCTCAGA CTCTCTGGCT 
CAATCAGTAA TTCAACATCT AAGATGGATA ATGCAGAAGG ATCTTTTGGG GCAAGACGTC 
TTTCTAATAG GACCTCCTGG GCCTCTTCGA CGCTCTATTG CTATGCAGTA CCTGGAGCTG 
ACCAAAAGGG AGGTTGAATA CATTGCCTTG TCAAGAGACA CCACCGAAAC CGACCTCAAA 
CAGCGGCGAG AGATCCGTGC TGGCACGGCT TTTTACATCG ATCAGTGTGC AGTTCGTGCA 
GCCACAGAAG GTAGAACACT CATCCTGGAA GGCTTGGAAA AGGCGGAGAG GAACGTCCTG 
CCGGTTTTGA ACAACTTGCT GGAGAACAGG GAGATGCAGC TTGAGGATGG ACGCTTCCTG 
ATGTCTGCCG AGCGTTACGA CAAACTTCTC CAAGATCATA CCAAAACAGA ATTGGATGCT 
TGGAAGATTG TCCGAGTTAG TGAAAATTTC CGAGTCATTG CCTTGGGCTT GCCAGTGCCC 
AGGTACTCTG GGAATCCATT AGATCCCCCA CTCCGGTCTC GATTTCAAGC CAGAGATATT 
TATTATCTGC CCTTCAAGGA CCAACTTAAA CTGTTATACT CAGTTGGAGC CAATGTTCCT 
ACTGAAAAAG TTTCTCAGCT CTTATCCTTT GCCACCACTC TCTGTTCCCA AGAATCTTCT 
ACCCTTGGAC TTCCAGATTT TCCTTTAGAT AGTTTACCAG CTGCAGTTCA AATCCTGGAT 
TCCTTTCCTA TGATGTCAAT CCAACACGTG CTCCAGCGGC TTTACCCGTA CACTGTGTTA 
CTGGGCCATG AAGGGAGGAT GGCTGTGCAA GGGGTTTTAA AACGCTTTGA ACTTGAGGAT 
TCAAGAAGAT CTGTGCTTCC TGAGAAGATT GTGAGAGTGG AAAGGATGAC CGAAAACAAT 
GTCTTCCAAG CTTCTGTGAC CATCCGGATT GCAGAAAAAG AGGTGACCAT CAAGGTACCA 
GCTGGGACTA GGCCATTAAG TCAGCCTTGT ACATCGGACC GTTTTATACA AACCTTGAGC 
CATAAACAAC TACTTGCTGA AATGATTCAG TCTCACATGG TTAAGGACAT ATGCTTAATT 
GGAGGAAAAG GTTGTGGAAA AACAGTCATC ACTAAGAACT TTGCAGATAC CTTGGGATAC 
AATATAGAAC CTATCATGCT GTATCAGGAT ATGACAGCGC GTGATCTGCT ACAGCAGAGG 
TACACGCTTC CCAATGGAGA CACTGCCTGG CGGTCCTCGC CCCTTGTAAA CGCTGCTCTG 
GAAGGAAAGC TGGTTCTCCT GGATGGCATT CACAGAGTCA ATGCCGGGAC GCTTGCCGTG 
TTACAGAGGC TGATCCATGA TCGGGAGCTA AGCCTGTATG ATGGCTCCAG GCTGCTGAGA 
GAAGACAGGT ACTTGCGCTT GAAGGAGGAG CTGCAAATGT CTGATGAGCA GCTGCAGCAG 
AGATCGATTT TTCCTGTTCA CCCCTCCTTC AGAATCATCG CCTTGGCAGA ACCCCCTGTT 
ATTGGAAGCA CGACACAGCA GTGGCTGGGG CCAGAATTCT TAACCATGTT CTTTTTCCAT 
TACATGAAAC CACTTGTCAA AAGTGAAGAA ATCCAAGTGA TTAAGGAAAT GGTCCCAGAT 
ATGCCTCCGG AAGCTTTGGA CAAATTGTTG TCATTTACGC ACAAACTCAG AGAGACACAG 
GATCCAACAG CGCAATCACT GGCGGCGTCC CTTTCTACCA GACAGCTGTT GAGGATTTCT 
CGTCGGCTGT CACAGTACCC TAATGAGAAC CTTCACAATG CTGTCACCAA AGCCTGTCTT 
TCCAGGTTTT TACCAAGCCT TGCAAGGTCA GCATTAGAAA AAAATCTGGC AGATGCTGCA 
ATAGAAATAA GCACTGATAA TAGTCCAGAG TCTGAATTGG AGGATTACAA ATGTGAAGTT 
TCTTCTGGAT CTCTGAGGAT TGGCACGGTT AGTGCACCAA TCTATAATGC CCACGAGAAA 
ATGAAAGTGC CTGATGTTTT GTTCTATGAC AACATCCAGC ACATGATAGT GATGGAAGAT 
ATGCTCAAAG ACTTTCTTCT TGGAGAACAC CTATTATTGG TTGGAAACCA GGGTGTAGGG 
AAAAACAAGA TTGTTGATAG ATTCCTTCAC CTGCTCAACA GACCCCGAGA GTATATCCAG 
CTGCACAGGG ACACCACAGT CCAAACACTT ACTCTTCAGC CTTCTGTTAA AGATGGACTT 
ATTGTATATG AAGACTCACC TTTGGTTAAA GCAGTAAAAT TGGGTCATGT TCTGGTAGTA 
GATGAGGCAG ACAAGGCTCC AACAAATGTC ACCTGTATTT TAAAAACTCT AGTAGAAAAT 
GGAGAAATGA TTCTAGCAGA TGGACGACGC ATTGTTGCAA ATGCTGCACT AGTCAATGGA 
AGAGAAAATG TTGTAGTGAT TCATCCTGAT TTTAGAATGA TTGTTCTGGC CAACAGACCT 
GGATTTCCTT TCCTAGGCAA TGATTTCTTC GGTACCTTAG GTGATATTTT TAGCTGCCAT 
GCAGTGGATA ATCCCAAACC CTACTCGGAA CTCAGGATGC TCAGGCAGTA CGGCCCAAAT 
GTGCCCGAGC CCATCCTTCA GAAGCTCGTC GCTGCCTTTG GAGAACTGAG GAATTTAGCT 
GACCAGGGTA TTATTAACTA CCCTTATTCT ACCAGAGAAG TTGTCAACAT AGTCAAGCAT 
TTGCAGAAGT TTCCCACTGA AGGTCTGTCC AGTGTGGTTA GGAACGTGTT TGACTTTGAT 
TCCTACAACA ACGACATGAG GGAGATTTTG ATTAACACTT TGCATAAATA CGGAATACCA 
GTTGGAGCAA AACCATCCAA TGTGCAGCTG GCGAAGGAGT TGCCTCTGCC GGAGCAGACC 
TTCATGGGCT ACTGGACAAT TGGTCAGTCA AGAAATGGAA TGCAAAAACT GCTGTGTCCA 
GCAGAAACTC ATCATATAGA CGTTAAGGGT CCAGCACTCA TACACATACA GGAGTATCCA 
ATAGAAAGAC ATGAAGGAAG ATCCCAAACC TTTACCGAAG AATGTAGCTC CTGGAGGTTA 
CCATTGGATG AAATGAACTT AATCTGTGAC GTAGCTACAG CACATGAAAA TGAGGAAAAT 
ACTCTCTATG TAGTTGCATG CAATCCCGTG TCCTTATACT TTATGAATAT GACTGGGAAG 
AGCGGCCACT TTGTGGACCT TTTCGACATC TTCCCGAGAA CGGCCAGTGG GATCTGGCAC 
CCATTTGTGA CGGTGGCACC GCTGGGAAAT CCCCTAAAGG GTCAAGTGGT TCTCCATGAG 
CAGCAGAGCA ATGTCATCCT GTTGCTGGAT CCCGCTAGCC AAGCCCTTCG CCGTCTCATC 
CTACCTTCAG AAGAATTTAC AATGAAGAAA ACTTCCTGGT GGAACAAGGA GGAAGCTGAA 
ACTTACAAAA TGTGTAAAGA GTTTTCACAC AAAAACTGGC TAGTGTTCTA CAAAGAAAAA 
GGGAACACCC TCGCTGTGCT GGATGTTCTA GAAGGGCGAA CTCACACCAT CTCGCTTCCC 
ATCAACCTCC AGACAGTTTT TCTCGTAGCA GAAGACAAAT GGCTTCTGGT GGAGAGTAAA 
ACAAATCAGA AGTATCTTTT AACTACACCT GCACACATCG AATCTAAGGA CAGTGGGGTT 
TGCCAGTTGT ATGTGTTGAA GGAGGAGCTT CCTAGCCCAG GGTTTGGAGT CATGCAAGAA 
ACAGACCGCA GCATACCTCA TACAATTTCA AGTGATCAAC TGTCATCTGA AAATCTGAGT 
TCAGCTGTGG GACAGAAGAT TTCCTCTCCA AACCGACTTC TCTCAGATAA GAATAGTTAT 
GCTACAATTG TTGTTGGTTT CCCAGATCTC ATGTCACCTA GTGAAGTTTA TTCTTGGAAA 
AGGCCATCAT CTTTGAGTAA ATCAAGAGTC ACTGATACAA CATTCTATGG AGGAAAGAAG 
AAAAGTGGAA CCGCGAAACA GAGCAATTGT GTGACTCTTT TGGATACGAA TCAGATAGTC 
AGGATTTTGC CCCCCGGAGA AGTCCCTCTA CAAGACATCT ACCCAAAAGA TGTTACCCCT 
CCACAGACAG CTGGGTATAT AGAAGTTACT GATCTCCAGT CCAAGAGACT TCGATACATC 
CCAATTCCCG GGTCAGACTC TCTATCACCA TATACCACAT GGCTGTCTAC TATCTCGGAC 
ACAGATGCAC TGCTGGCCGA GTGGGGCAAA GGTGGCGTTG TCACTGTTGA TATGGGAGGC 
CGTGTCCGGC TTTGGGAAAC CGGCCTTGAG CACCTGCAGC GGTCACTCAT GGAATGGAGA 
AACATGATTG GAGAAGGTGA CAGACACATG CAGATAACAA TCGAAAGAGA GAGCGGTGAA 
GACGTGAGCT CCCCCAAACA CGGGAAGGAG GACCCAGACA ACATGCCACA TGTGGGTGGT 
AACACTTGGG CTGGCGGCAC AGGTGGAAGA GACACTGCAG GCCTGGGCGG CAAGGGAGGT 
CCTTACCGGC TGGATGCAGG CCACACGGTC TACCAGGTCT CTCAGGCCGA GAAAGACGCT 
GTTCCCGAGG AGGTGAAGAG AGCTGCGAGA GAGATGGGCC AGAGAGCGTT TCAACAGAGG 
CTAAAGGAGA TCCAAATGAG TGAATATGAT GCTGCCACCT ATGAAAGGTT TTCAGGGGCT 
GTTCGACGAC AGGTGCACTC CCTCCGAATT ATCCTGGATA ATTTACAGGC TAAAGGTAAA 
GAAAGACAGT GGCTAAGACA CCAGGCTACT GGGGAGCTAG ATGATGCCAA GATCATTGAT 
GGGCTAACTG GAGAAAAAGC CATCTACAAA CGCCGGGGTG AGCTGGAGCC ACAAATGGGC 
AGCCCCCAAC AGAAACCCAA GCGCCTGCGC CTGGTGGTAG ACGTGTCGGG CAGCATGTAC 
CGCTTCAACG GGGTGGATGG CCGGCTCGAG CGCTCAATGG AAGCTGTGTG CATGGTCATG 
GAGGCCTTCG AGAACTATGA GGAGAAGTTT AAGTATGACA TCGCTGGACA CTCTGGAGAT 
GGATACAACA TTGGTCTAGT TCCAATTAAC AAAATCCCCA AGGACAATAA GCAAAGACTA 
GAAATTCTCA AGACGATGCA CGCCCACGCT CAGTTCTGCA TGAGTGGGGA CCACACCTTA 
GAGGGGACAG AACATGCCAT CAAAGAAATC GTCAAAGAAG AAGCTGATGA GTACTTCGTC 
ATTGTCTTAA GTGATGCCAA CCTGTCTCGC TATGGAATAC ACCCTGCCAA GTTTGCCCAG 
ATCCTGACAA GCAACCCTCA AGTAAATGCT TTCGCCATCT TTATTGGATC TTTAGGCGAT 
CAAGCAGCCA GGCTTCAGAG AACTCTACCA GCGGGCCGGT CTTTCGTTGC CATGGACACC 
AAGGACATCC CTCAGATTTT ACAACAGATT TTCACCTCCA CCATGCTGTC CAGTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VWA8 ( XM_006076533.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006076595.1 Bubalus bubalis von Willebrand factor A domain containing 8 (VWA8), mRNA.

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CloneID	OBu30039
Clone ID Related Accession (Same CDS sequence)	XM_006076533.1
Accession Version	XM_006076533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	von Willebrand factor A domain-containing protein 8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785696.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACATTG GGGTACACCA TCTCCTGAAG TCCCCGAGCA CTGACGGGAC CGTATGGAGC 
CGCCGGAGGC CTGTCCGGGG AGTTCGGGGA CGCGCCGCAG GTGAGTGCAA GGCGCGCCCC 
GGGGTCACCG CCGGCACGCG CTCCTCCCCG CCGGCCCCGG GCACGGCTCC AGGCGACGAG 
GACCGGCACC CTAGCGGGCC CAGGGCCGCC CGGGTCGGCC GAGCCGGGCG CGCCGGGGGA 
AGGAGTTTCC GGCGGACTCC GCGCGCTGCC GCTTCCTGTC CCCCTCGTCG CGCCGCCTCA 
CGCCGCCCGT CCCGGAGCGC CATTACTGTA CACGCAGGGG TTCATGCAGC TGCCCTCAGA 
GGCTGCGGAA AGCTGGGCCT GACCTTGCTC TCCAAGTCTC CAGGACCTGG ATGGAAGTCA 
GTCCGTGCCA CGTTCCAGCC AGGGCTGGCG GCCGCTGACA AGTGTGATAC AGTTAATATT 
GGAGATGTAT CCTACAAGTT GAAAAATCCT AAGAACCCAG AACTTGTGCC ACAAAACTAC 
ATCTCAGACT CTCTGGCTCA ATCAGTAATT CAACATCTAA GATGGATAAT GCAGAAGGAT 
CTTTTGGGGC AAGACGTCTT TCTAATAGGA CCTCCTGGGC CTCTTCGACG CTCTATTGCT 
ATGCAGTACC TGGAGCTGAC CAAAAGGGAG GTTGAATACA TTGCCTTGTC AAGAGACACC 
ACCGAAACCG ACCTCAAACA GCGGCGAGAG ATCCGTGCTG GCACGGCTTT TTACATCGAT 
CAGTGTGCAG TTCGTGCAGC CACAGAAGGT AGAACACTCA TCCTGGAAGG CTTGGAAAAG 
GCGGAGAGGA ACGTCCTGCC GGTTTTGAAC AACTTGCTGG AGAACAGGGA GATGCAGCTT 
GAGGATGGAC GCTTCCTGAT GTCTGCCGAG CGTTACGACA AACTTCTCCA AGATCATACC 
AAAACAGAAT TGGATGCTTG GAAGATTGTC CGAGTTAGTG AAAATTTCCG AGTCATTGCC 
TTGGGCTTGC CAGTGCCCAG GTACTCTGGG AATCCATTAG ATCCCCCACT CCGGTCTCGA 
TTTCAAGCCA GAGATATTTA TTATCTGCCC TTCAAGGACC AACTTAAACT GTTATACTCA 
GTTGGAGCCA ATGTTCCTAC TGAAAAAGTT TCTCAGCTCT TATCCTTTGC CACCACTCTC 
TGTTCCCAAG AATCTTCTAC CCTTGGACTT CCAGATTTTC CTTTAGATAG TTTACCAGCT 
GCAGTTCAAA TCCTGGATTC CTTTCCTATG ATGTCAATCC AACACGTGCT CCAGCGGCTT 
TACCCGTACA CTGTGTTACT GGGCCATGAA GGGAGGATGG CTGTGCAAGG GGTTTTAAAA 
CGCTTTGAAC TTGAGGATTC AAGAAGATCT GTGCTTCCTG AGAAGATTGT GAGAGTGGAA 
AGGATGACCG AAAACAATGT CTTCCAAGCT TCTGTGACCA TCCGGATTGC AGAAAAAGAG 
GTGACCATCA AGGTACCAGC TGGGACTAGG CCATTAAGTC AGCCTTGTAC ATCGGACCGT 
TTTATACAAA CCTTGAGCCA TAAACAACTA CTTGCTGAAA TGATGCAGTC TCACATGGTT 
AAGGACATAT GCTTAATTGG AGGAAAAGGT TGTGGAAAAA CAGTCATCAC TAAGAACTTT 
GCAGATACCT TGGGATACAA TATAGAACCT ATCATGCTGT ATCAGGATAT GACAGCGCGT 
GATCTGCTAC AGCAGAGGTA CACGCTTCCC AATGGAGACA CTGCCTGGCG GTCCTCGCCC 
CTTGTAAACG CTGCTCTGGA AGGAAAGCTG GTTCTCCTGG ATGGCATTCA CAGAGTCAAT 
GCCGGGACGC TTGCCGTGTT ACAGAGGCTG ATCCATGATC GGGAGCTAAG CCTGTATGAT 
GGCTCCAGGC TGCTGAGAGA AGACAGGTAC TTGCGCTTGA AGGAGGAGCT GCAAATGTCT 
GATGAGCAGC TGCAGCAGAG ATCGATTTTT CCTGTTCACC CCTCCTTCAG AATCATCGCC 
TTGGCAGAAC CCCCTGTTAT TGGAAGCACG ACACAGCAGT GGCTGGGGCC AGAATTCTTA 
ACCATGTTCT TTTTCCATTA CATGAAACCA CTTGTCAAAA GTGAAGAAAT CCAAGTGATT 
AAGGAAATGG TCCCAGATAT GCCTCCGGAA GCTTTGGACA AATTGTTGTC ATTTACGCAC 
AAACTCAGAG AGACACAGGA TCCAACAGCG CAATCACTGG CGGCGTCCCT TTCTACCAGA 
CAGCTGTTGA GGATTTCTCG TCGGCTGTCA CAGTACCCTA ATGAGAACCT TCACAATGCT 
GTCACCAAAG CCTGTCTTTC CAGGTTTTTA CCAAGCCTTG CAAGGTCAGC ATTAGAAAAA 
AATCTGGCAG ATGCTGCAAT AGAAATAAGC ACTGATAATA GTCCAGAGTC TGAATTGGAG 
GATTACAAAT GTGAAGTTTC TTCTGGATCT CTGAGGATTG GCACGGTTAG TGCACCAATC 
TATAATGCCC ACGAGAAAAT GAAAGTGCCT GATGTTTTGT TCTATGACAA CATCCAGCAC 
ATGATAGTGA TGGAAGATAT GCTCAAAGAC TTTCTTCTTG GAGAACACCT ATTATTGGTT 
GGAAACCAGG GTGTAGGGAA AAACAAGATT GTTGATAGAT TCCTTCACCT GCTCAACAGA 
CCCCGAGAGT ATATCCAGCT GCACAGGGAC ACCACAGTCC AAACACTTAC TCTTCAGCCT 
TCTGTTAAAG ATGGACTTAT TGTATATGAA GACTCACCTT TGGTTAAAGC AGTAAAATTG 
GGTCATGTTC TGGTAGTAGA TGAGGCAGAC AAGGCTCCAA CAAATGTCAC CTGTATTTTA 
AAAACTCTAG TAGAAAATGG AGAAATGATT CTAGCAGATG GACGACGCAT TGTTGCAAAT 
GCTGCACTAG TCAATGGAAG AGAAAATGTT GTAGTGATTC ATCCTGATTT TAGAATGATT 
GTTCTGGCCA ACAGACCTGG ATTTCCTTTC CTAGGCAATG ATTTCTTCGG TACCTTAGGT 
GATATTTTTA GCTGCCATGC AGTGGATAAT CCCAAACCCT ACTCGGAACT CAGGATGCTC 
AGGCAGTACG GCCCAAATGT GCCCGAGCCC ATCCTTCAGA AGCTCGTCGC TGCCTTTGGA 
GAACTGAGGA ATTTAGCTGA CCAGGGTATT ATTAACTACC CTTATTCTAC CAGAGAAGTT 
GTCAACATAG TCAAGCATTT GCAGAAGTTT CCCACTGAAG GTCTGTCCAG TGTGGTTAGG 
AACGTGTTTG ACTTTGATTC CTACAACAAC GACATGAGGG AGATTTTGAT TAACACTTTG 
CATAAATACG GAATACCAGT TGGAGCAAAA CCATCCAATG TGCAGCTGGC GAAGGAGTTG 
CCTCTGCCGG AGCAGACCTT CATGGGCTAC TGGACAATTG GTCAGTCAAG AAATGGAATG 
CAAAAACTGC TGTGTCCAGC AGAAACTCAT CATATAGACG TTAAGGGTCC AGCACTCATA 
CACATACAGG AGTATCCAAT AGAAAGACAT GAAGGAAGAT CCCAAACCTT TACCGAAGAA 
TGTAGCTCCT GGAGGTTACC ATTGGATGAA ATGAACTTAA TCTGTGACGT CGCTACAGCA 
CATGAAAATG AGGAAAATAC TCTCTATGTA GTTGCATGCA ATCCCGTGTC CTTATACTTT 
ATGAATATGA CTGGGAAGAG CGGCCACTTT GTGGACCTTT TCGACATCTT CCCGAGAACG 
GCCAGTGGGA TCTGGCACCC ATTTGTGACG GTGGCGCCGC TGGGAAATCC CCTAAAGGGT 
CAAGTGGTTC TCCATGAGCA GCAGAGCAAT GTCATCCTGT TGCTGGATCC CGCTAGCCAA 
GCCCTTCGCC GTCTCATCCT ACCTTCAGAA GAATTTACAA TGAAGAAAAC TTCCTGGTGG 
AACAAGGAGG AAGCTGAAAC TTACAAAATG TGTAAAGAGT TTTCACACAA AAACTGGCTA 
GTGTTCTACA AAGAAAAAGG GAACACCCTC GCTGTGCTGG ATGTTCTAGA AGGGCGAACT 
CACACCATCT CGCTTCCCAT CAACCTCCAG ACGGTTTTTC TCGTAGCAGA AGACAAATGG 
CTTCTGGTGG AGAGTAAAAC AAATCAGAAG TATCTTTTAA CTACACCTGC ACACATCGAA 
TCTAAGGACA GTGGGGTTTG CCAGTTGTAT GTGTTGAAGG AGGAGCTTCC TAGCCCAGGG 
TTTGGAGTCA TGCAAGAAAC AGACCGCAGC ATACCTCATA CAATTTCAAG TGATCAACTG 
TCATCTGAAA ATCTGAGTTC AGCTGTGGGA CAGAAGATTT CCTCTCCAAA CCGACTTCTC 
TCAGATAAGA ATAGTTATGC TACAATTGTT GTTGGTTTCC CAGATCTCAT GTCACCTAGT 
GAAGTTTATT CTTGGAAAAG GCCATCATCT TTGAGTAAAT CAAGAGTCAC TGATACAACA 
TTCTATGGAG GAAAGAAGAA AAGTGGAACC GCGAAACAGA GCAATTGTGT GACTCTTTTG 
GATACGAATC AGATAGTCAG GATTTTGCCC CCCGGAGAAG TCCCTCTACA AGACATCTAC 
CCAAAAGATG TTACCCCTCC ACAGACAGCT GGGTATATAG AAGTTACTGA TCTCCAGTCC 
AAGAGACTTC GATACATCCC AATTCCCGGG TCAGACTCTC TATCACCATA TACCACATGG 
CTGTCTACTA TCTCGGACAC AGACGCACTG CTGGCCGAGT GGGGCAAAGG TGGCGTTGTC 
ACTGTTGATA TGGGAGGCCG TGTCCGGCTT TGGGAAACCG GCCTTGAGCA CCTGCAGCGG 
TCACTCATGG AATGGAGAAA CATGATTGGA GAAGGTGACA GACACATGCA GATAACAATC 
GAAAGAGAGA GCGGTGAAGA CGTGAGCTCC CCCAAACACG GGAAGGAGGA CCCAGACAAC 
ATGCCACATG TGGGTGGTAA CACTTGGGCT GGCGGCACAG GTGGAAGAGA CACTGCAGGC 
CTGGGCGGCA AGGGAGGTCC TTACCGGCTG GATGCAGGCC ACACGGTCTA CCAGGTCTCT 
CAGGCCGAGA AAGACGCTGT TCCCGAGGAG GTGAAGAGAG CTGCGAGAGA GATGGGCCAG 
AGAGCGTTTC AACAGAGGCT AAAGGAGATC CAAATGAGTG AATATGATGC TGCCACCTAT 
GAAAGGTTTT CAGGGGCTGT TCGACGACAG GTGCACTCCC TCCGAATTAT CCTGGATAAT 
TTACAGGCTA AAGGTAAAGA AAGACAGTGG CTAAGACACC AGGCTACTGG GGAGCTAGAT 
GATGCCAAGA TCATTGATGG GCTAACTGGA GAAAAAGCCA TCTACAAACG CCGGGGTGAG 
CTGGAGCCAC AAATGGGCAG CCCCCAACAG AAACCCAAGC GCCTGCGCCT GGTGGTAGAC 
GTGTCGGGCA GCATGTACCG CTTCAACGGG GTGGATGGCC GGCTCGAGCG CTCAATGGAA 
GCTGTGTGCA TGGTCATGGA GGCCTTCGAG AACTATGAGG AGAAGTTTAA GTATGACATC 
GCTGGACACT CTGGAGATGG ATACAACATT GGTCTAGTTC CAATTAACAA AATCCCCAAG 
GACAATAAGC AAAGACTAGA AATTCTCAAG ACGATGCATG CCCACGCTCA GTTCTGCATG 
AGTGGGGACC ACACCTTAGA GGGGACAGAA CATGCCATCA AAGAAATCGT CAAAGAAGAA 
GCTGATGAGT ACTTCGTCAT TGTCTTAAGT GATGCCAACC TGTCTCGCTA TGGAATACAC 
CCTGCCAAGT TTGCCCAGAT CCTGACAAGC AACCCTCAAG TAAATGCTTT CGCCATCTTT 
ATTGGATCTT TAGGCGATCA AGCAGCCAGG CTTCAGAGAA CTCTACCAGC GGGCCGGTCT 
TTCGTTGCCA TGGACACCAA GGACATCCCT CAGATTTTAC AACAGATTTT CACCTCCACC 
ATGCTGTCCA GTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006076595.1
CDS	1..6018
Translation	MNIGVHHLLKSPSTDGTVWSRRRPVRGVRGRAAGECKARPGVTAGTRSSPPAPGTAPGDEDRHPSGPRAARVGRAGRAGGRSFRRTPRAAASCPPRRAASRRPSRSAITVHAGVHAAALRGCGKLGLTLLSKSPGPGWKSVRATFQPGLAAADKCDTVNIGDVSYKLKNPKNPELVPQNYISDSLAQSVIQHLRWIMQKDLLGQDVFLIGPPGPLRRSIAMQYLELTKREVEYIALSRDTTETDLKQRREIRAGTAFYIDQCAVRAATEGRTLILEGLEKAERNVLPVLNNLLENREMQLEDGRFLMSAERYDKLLQDHTKTELDAWKIVRVSENFRVIALGLPVPRYSGNPLDPPLRSRFQARDIYYLPFKDQLKLLYSVGANVPTEKVSQLLSFATTLCSQESSTLGLPDFPLDSLPAAVQILDSFPMMSIQHVLQRLYPYTVLLGHEGRMAVQGVLKRFELEDSRRSVLPEKIVRVERMTENNVFQASVTIRIAEKEVTIKVPAGTRPLSQPCTSDRFIQTLSHKQLLAEMMQSHMVKDICLIGGKGCGKTVITKNFADTLGYNIEPIMLYQDMTARDLLQQRYTLPNGDTAWRSSPLVNAALEGKLVLLDGIHRVNAGTLAVLQRLIHDRELSLYDGSRLLREDRYLRLKEELQMSDEQLQQRSIFPVHPSFRIIALAEPPVIGSTTQQWLGPEFLTMFFFHYMKPLVKSEEIQVIKEMVPDMPPEALDKLLSFTHKLRETQDPTAQSLAASLSTRQLLRISRRLSQYPNENLHNAVTKACLSRFLPSLARSALEKNLADAAIEISTDNSPESELEDYKCEVSSGSLRIGTVSAPIYNAHEKMKVPDVLFYDNIQHMIVMEDMLKDFLLGEHLLLVGNQGVGKNKIVDRFLHLLNRPREYIQLHRDTTVQTLTLQPSVKDGLIVYEDSPLVKAVKLGHVLVVDEADKAPTNVTCILKTLVENGEMILADGRRIVANAALVNGRENVVVIHPDFRMIVLANRPGFPFLGNDFFGTLGDIFSCHAVDNPKPYSELRMLRQYGPNVPEPILQKLVAAFGELRNLADQGIINYPYSTREVVNIVKHLQKFPTEGLSSVVRNVFDFDSYNNDMREILINTLHKYGIPVGAKPSNVQLAKELPLPEQTFMGYWTIGQSRNGMQKLLCPAETHHIDVKGPALIHIQEYPIERHEGRSQTFTEECSSWRLPLDEMNLICDVATAHENEENTLYVVACNPVSLYFMNMTGKSGHFVDLFDIFPRTASGIWHPFVTVAPLGNPLKGQVVLHEQQSNVILLLDPASQALRRLILPSEEFTMKKTSWWNKEEAETYKMCKEFSHKNWLVFYKEKGNTLAVLDVLEGRTHTISLPINLQTVFLVAEDKWLLVESKTNQKYLLTTPAHIESKDSGVCQLYVLKEELPSPGFGVMQETDRSIPHTISSDQLSSENLSSAVGQKISSPNRLLSDKNSYATIVVGFPDLMSPSEVYSWKRPSSLSKSRVTDTTFYGGKKKSGTAKQSNCVTLLDTNQIVRILPPGEVPLQDIYPKDVTPPQTAGYIEVTDLQSKRLRYIPIPGSDSLSPYTTWLSTISDTDALLAEWGKGGVVTVDMGGRVRLWETGLEHLQRSLMEWRNMIGEGDRHMQITIERESGEDVSSPKHGKEDPDNMPHVGGNTWAGGTGGRDTAGLGGKGGPYRLDAGHTVYQVSQAEKDAVPEEVKRAAREMGQRAFQQRLKEIQMSEYDAATYERFSGAVRRQVHSLRIILDNLQAKGKERQWLRHQATGELDDAKIIDGLTGEKAIYKRRGELEPQMGSPQQKPKRLRLVVDVSGSMYRFNGVDGRLERSMEAVCMVMEAFENYEEKFKYDIAGHSGDGYNIGLVPINKIPKDNKQRLEILKTMHAHAQFCMSGDHTLEGTEHAIKEIVKEEADEYFVIVLSDANLSRYGIHPAKFAQILTSNPQVNAFAIFIGSLGDQAARLQRTLPAGRSFVAMDTKDIPQILQQIFTSTMLSSV

Target ORF information:

RefSeq Version	XM_006076533.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis von Willebrand factor A domain containing 8 (VWA8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006076533.1

ORF Insert Sequence:

ATGAACATTG GGGTACACCA TCTCCTGAAG TCCCCGAGCA CTGACGGGAC CGTATGGAGC 
CGCCGGAGGC CTGTCCGGGG AGTTCGGGGA CGCGCCGCAG GTGAGTGCAA GGCGCGCCCC 
GGGGTCACCG CCGGCACGCG CTCCTCCCCG CCGGCCCCGG GCACGGCTCC AGGCGACGAG 
GACCGGCACC CTAGCGGGCC CAGGGCCGCC CGGGTCGGCC GAGCCGGGCG CGCCGGGGGA 
AGGAGTTTCC GGCGGACTCC GCGCGCTGCC GCTTCCTGTC CCCCTCGTCG CGCCGCCTCA 
CGCCGCCCGT CCCGGAGCGC CATTACTGTA CACGCAGGGG TTCATGCAGC TGCCCTCAGA 
GGCTGCGGAA AGCTGGGCCT GACCTTGCTC TCCAAGTCTC CAGGACCTGG ATGGAAGTCA 
GTCCGTGCCA CGTTCCAGCC AGGGCTGGCG GCCGCTGACA AGTGTGATAC AGTTAATATT 
GGAGATGTAT CCTACAAGTT GAAAAATCCT AAGAACCCAG AACTTGTGCC ACAAAACTAC 
ATCTCAGACT CTCTGGCTCA ATCAGTAATT CAACATCTAA GATGGATAAT GCAGAAGGAT 
CTTTTGGGGC AAGACGTCTT TCTAATAGGA CCTCCTGGGC CTCTTCGACG CTCTATTGCT 
ATGCAGTACC TGGAGCTGAC CAAAAGGGAG GTTGAATACA TTGCCTTGTC AAGAGACACC 
ACCGAAACCG ACCTCAAACA GCGGCGAGAG ATCCGTGCTG GCACGGCTTT TTACATCGAT 
CAGTGTGCAG TTCGTGCAGC CACAGAAGGT AGAACACTCA TCCTGGAAGG CTTGGAAAAG 
GCGGAGAGGA ACGTCCTGCC GGTTTTGAAC AACTTGCTGG AGAACAGGGA GATGCAGCTT 
GAGGATGGAC GCTTCCTGAT GTCTGCCGAG CGTTACGACA AACTTCTCCA AGATCATACC 
AAAACAGAAT TGGATGCTTG GAAGATTGTC CGAGTTAGTG AAAATTTCCG AGTCATTGCC 
TTGGGCTTGC CAGTGCCCAG GTACTCTGGG AATCCATTAG ATCCCCCACT CCGGTCTCGA 
TTTCAAGCCA GAGATATTTA TTATCTGCCC TTCAAGGACC AACTTAAACT GTTATACTCA 
GTTGGAGCCA ATGTTCCTAC TGAAAAAGTT TCTCAGCTCT TATCCTTTGC CACCACTCTC 
TGTTCCCAAG AATCTTCTAC CCTTGGACTT CCAGATTTTC CTTTAGATAG TTTACCAGCT 
GCAGTTCAAA TCCTGGATTC CTTTCCTATG ATGTCAATCC AACACGTGCT CCAGCGGCTT 
TACCCGTACA CTGTGTTACT GGGCCATGAA GGGAGGATGG CTGTGCAAGG GGTTTTAAAA 
CGCTTTGAAC TTGAGGATTC AAGAAGATCT GTGCTTCCTG AGAAGATTGT GAGAGTGGAA 
AGGATGACCG AAAACAATGT CTTCCAAGCT TCTGTGACCA TCCGGATTGC AGAAAAAGAG 
GTGACCATCA AGGTACCAGC TGGGACTAGG CCATTAAGTC AGCCTTGTAC ATCGGACCGT 
TTTATACAAA CCTTGAGCCA TAAACAACTA CTTGCTGAAA TGATGCAGTC TCACATGGTT 
AAGGACATAT GCTTAATTGG AGGAAAAGGT TGTGGAAAAA CAGTCATCAC TAAGAACTTT 
GCAGATACCT TGGGATACAA TATAGAACCT ATCATGCTGT ATCAGGATAT GACAGCGCGT 
GATCTGCTAC AGCAGAGGTA CACGCTTCCC AATGGAGACA CTGCCTGGCG GTCCTCGCCC 
CTTGTAAACG CTGCTCTGGA AGGAAAGCTG GTTCTCCTGG ATGGCATTCA CAGAGTCAAT 
GCCGGGACGC TTGCCGTGTT ACAGAGGCTG ATCCATGATC GGGAGCTAAG CCTGTATGAT 
GGCTCCAGGC TGCTGAGAGA AGACAGGTAC TTGCGCTTGA AGGAGGAGCT GCAAATGTCT 
GATGAGCAGC TGCAGCAGAG ATCGATTTTT CCTGTTCACC CCTCCTTCAG AATCATCGCC 
TTGGCAGAAC CCCCTGTTAT TGGAAGCACG ACACAGCAGT GGCTGGGGCC AGAATTCTTA 
ACCATGTTCT TTTTCCATTA CATGAAACCA CTTGTCAAAA GTGAAGAAAT CCAAGTGATT 
AAGGAAATGG TCCCAGATAT GCCTCCGGAA GCTTTGGACA AATTGTTGTC ATTTACGCAC 
AAACTCAGAG AGACACAGGA TCCAACAGCG CAATCACTGG CGGCGTCCCT TTCTACCAGA 
CAGCTGTTGA GGATTTCTCG TCGGCTGTCA CAGTACCCTA ATGAGAACCT TCACAATGCT 
GTCACCAAAG CCTGTCTTTC CAGGTTTTTA CCAAGCCTTG CAAGGTCAGC ATTAGAAAAA 
AATCTGGCAG ATGCTGCAAT AGAAATAAGC ACTGATAATA GTCCAGAGTC TGAATTGGAG 
GATTACAAAT GTGAAGTTTC TTCTGGATCT CTGAGGATTG GCACGGTTAG TGCACCAATC 
TATAATGCCC ACGAGAAAAT GAAAGTGCCT GATGTTTTGT TCTATGACAA CATCCAGCAC 
ATGATAGTGA TGGAAGATAT GCTCAAAGAC TTTCTTCTTG GAGAACACCT ATTATTGGTT 
GGAAACCAGG GTGTAGGGAA AAACAAGATT GTTGATAGAT TCCTTCACCT GCTCAACAGA 
CCCCGAGAGT ATATCCAGCT GCACAGGGAC ACCACAGTCC AAACACTTAC TCTTCAGCCT 
TCTGTTAAAG ATGGACTTAT TGTATATGAA GACTCACCTT TGGTTAAAGC AGTAAAATTG 
GGTCATGTTC TGGTAGTAGA TGAGGCAGAC AAGGCTCCAA CAAATGTCAC CTGTATTTTA 
AAAACTCTAG TAGAAAATGG AGAAATGATT CTAGCAGATG GACGACGCAT TGTTGCAAAT 
GCTGCACTAG TCAATGGAAG AGAAAATGTT GTAGTGATTC ATCCTGATTT TAGAATGATT 
GTTCTGGCCA ACAGACCTGG ATTTCCTTTC CTAGGCAATG ATTTCTTCGG TACCTTAGGT 
GATATTTTTA GCTGCCATGC AGTGGATAAT CCCAAACCCT ACTCGGAACT CAGGATGCTC 
AGGCAGTACG GCCCAAATGT GCCCGAGCCC ATCCTTCAGA AGCTCGTCGC TGCCTTTGGA 
GAACTGAGGA ATTTAGCTGA CCAGGGTATT ATTAACTACC CTTATTCTAC CAGAGAAGTT 
GTCAACATAG TCAAGCATTT GCAGAAGTTT CCCACTGAAG GTCTGTCCAG TGTGGTTAGG 
AACGTGTTTG ACTTTGATTC CTACAACAAC GACATGAGGG AGATTTTGAT TAACACTTTG 
CATAAATACG GAATACCAGT TGGAGCAAAA CCATCCAATG TGCAGCTGGC GAAGGAGTTG 
CCTCTGCCGG AGCAGACCTT CATGGGCTAC TGGACAATTG GTCAGTCAAG AAATGGAATG 
CAAAAACTGC TGTGTCCAGC AGAAACTCAT CATATAGACG TTAAGGGTCC AGCACTCATA 
CACATACAGG AGTATCCAAT AGAAAGACAT GAAGGAAGAT CCCAAACCTT TACCGAAGAA 
TGTAGCTCCT GGAGGTTACC ATTGGATGAA ATGAACTTAA TCTGTGACGT CGCTACAGCA 
CATGAAAATG AGGAAAATAC TCTCTATGTA GTTGCATGCA ATCCCGTGTC CTTATACTTT 
ATGAATATGA CTGGGAAGAG CGGCCACTTT GTGGACCTTT TCGACATCTT CCCGAGAACG 
GCCAGTGGGA TCTGGCACCC ATTTGTGACG GTGGCGCCGC TGGGAAATCC CCTAAAGGGT 
CAAGTGGTTC TCCATGAGCA GCAGAGCAAT GTCATCCTGT TGCTGGATCC CGCTAGCCAA 
GCCCTTCGCC GTCTCATCCT ACCTTCAGAA GAATTTACAA TGAAGAAAAC TTCCTGGTGG 
AACAAGGAGG AAGCTGAAAC TTACAAAATG TGTAAAGAGT TTTCACACAA AAACTGGCTA 
GTGTTCTACA AAGAAAAAGG GAACACCCTC GCTGTGCTGG ATGTTCTAGA AGGGCGAACT 
CACACCATCT CGCTTCCCAT CAACCTCCAG ACGGTTTTTC TCGTAGCAGA AGACAAATGG 
CTTCTGGTGG AGAGTAAAAC AAATCAGAAG TATCTTTTAA CTACACCTGC ACACATCGAA 
TCTAAGGACA GTGGGGTTTG CCAGTTGTAT GTGTTGAAGG AGGAGCTTCC TAGCCCAGGG 
TTTGGAGTCA TGCAAGAAAC AGACCGCAGC ATACCTCATA CAATTTCAAG TGATCAACTG 
TCATCTGAAA ATCTGAGTTC AGCTGTGGGA CAGAAGATTT CCTCTCCAAA CCGACTTCTC 
TCAGATAAGA ATAGTTATGC TACAATTGTT GTTGGTTTCC CAGATCTCAT GTCACCTAGT 
GAAGTTTATT CTTGGAAAAG GCCATCATCT TTGAGTAAAT CAAGAGTCAC TGATACAACA 
TTCTATGGAG GAAAGAAGAA AAGTGGAACC GCGAAACAGA GCAATTGTGT GACTCTTTTG 
GATACGAATC AGATAGTCAG GATTTTGCCC CCCGGAGAAG TCCCTCTACA AGACATCTAC 
CCAAAAGATG TTACCCCTCC ACAGACAGCT GGGTATATAG AAGTTACTGA TCTCCAGTCC 
AAGAGACTTC GATACATCCC AATTCCCGGG TCAGACTCTC TATCACCATA TACCACATGG 
CTGTCTACTA TCTCGGACAC AGACGCACTG CTGGCCGAGT GGGGCAAAGG TGGCGTTGTC 
ACTGTTGATA TGGGAGGCCG TGTCCGGCTT TGGGAAACCG GCCTTGAGCA CCTGCAGCGG 
TCACTCATGG AATGGAGAAA CATGATTGGA GAAGGTGACA GACACATGCA GATAACAATC 
GAAAGAGAGA GCGGTGAAGA CGTGAGCTCC CCCAAACACG GGAAGGAGGA CCCAGACAAC 
ATGCCACATG TGGGTGGTAA CACTTGGGCT GGCGGCACAG GTGGAAGAGA CACTGCAGGC 
CTGGGCGGCA AGGGAGGTCC TTACCGGCTG GATGCAGGCC ACACGGTCTA CCAGGTCTCT 
CAGGCCGAGA AAGACGCTGT TCCCGAGGAG GTGAAGAGAG CTGCGAGAGA GATGGGCCAG 
AGAGCGTTTC AACAGAGGCT AAAGGAGATC CAAATGAGTG AATATGATGC TGCCACCTAT 
GAAAGGTTTT CAGGGGCTGT TCGACGACAG GTGCACTCCC TCCGAATTAT CCTGGATAAT 
TTACAGGCTA AAGGTAAAGA AAGACAGTGG CTAAGACACC AGGCTACTGG GGAGCTAGAT 
GATGCCAAGA TCATTGATGG GCTAACTGGA GAAAAAGCCA TCTACAAACG CCGGGGTGAG 
CTGGAGCCAC AAATGGGCAG CCCCCAACAG AAACCCAAGC GCCTGCGCCT GGTGGTAGAC 
GTGTCGGGCA GCATGTACCG CTTCAACGGG GTGGATGGCC GGCTCGAGCG CTCAATGGAA 
GCTGTGTGCA TGGTCATGGA GGCCTTCGAG AACTATGAGG AGAAGTTTAA GTATGACATC 
GCTGGACACT CTGGAGATGG ATACAACATT GGTCTAGTTC CAATTAACAA AATCCCCAAG 
GACAATAAGC AAAGACTAGA AATTCTCAAG ACGATGCATG CCCACGCTCA GTTCTGCATG 
AGTGGGGACC ACACCTTAGA GGGGACAGAA CATGCCATCA AAGAAATCGT CAAAGAAGAA 
GCTGATGAGT ACTTCGTCAT TGTCTTAAGT GATGCCAACC TGTCTCGCTA TGGAATACAC 
CCTGCCAAGT TTGCCCAGAT CCTGACAAGC AACCCTCAAG TAAATGCTTT CGCCATCTTT 
ATTGGATCTT TAGGCGATCA AGCAGCCAGG CTTCAGAGAA CTCTACCAGC GGGCCGGTCT 
TTCGTTGCCA TGGACACCAA GGACATCCCT CAGATTTTAC AACAGATTTT CACCTCCACC 
ATGCTGTCCA GTGTTTAA