VWDE cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	VWDE
Entrez Gene ID	102402014
Full Name	von Willebrand factor D and EGF domains
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_025291968.1	XP_025147753.1	LOW QUALITY PROTEIN: von Willebrand factor D and EGF domain-containing protein
XM_006072501.1	XP_006072563.1	LOW QUALITY PROTEIN: von Willebrand factor D and EGF domain-containing protein

Pathways from BioSystems
Gene Ontology

The following VWDE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VWDE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu26744	XM_006072501.1 Latest version!	Bubalus bubalis von Willebrand factor D and EGF domains (VWDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu71343	XM_025291968.1 Latest version!	Bubalus bubalis von Willebrand factor D and EGF domains (VWDE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VWDE ( XM_006072501.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006072563.1 Bubalus bubalis von Willebrand factor D and EGF domains (VWDE), mRNA.

Price & Availability↑

CloneID	OBu26744
Clone ID Related Accession (Same CDS sequence)	XM_006072501.1
Accession Version	XM_006072501.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3630bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: von Willebrand factor D and EGF domain-containing protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785493.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAAAA TTTATAAAGA CATCTATCTT TCCAATCTTG CTTGGGAGTG CTTTCCTGGG 
GGACACCAGT TTCTTCAGAG CCCTTATAAA AATGTCCATT TTGACTCCTT GCACCTCCAA 
TAGTCAGCCA TTCAAGACCT AATATGTGAC CATTCCCTTT CTCCTGGATG GTATCTATTT 
CTGCTCTTTG ATAGACCTGC TGAGATGCCG ACCAAATGTG TTGAGATGAA CCACTGTGGT 
TCATCTGCCC CCATCTGGCT GTCTCTGAAA GATTCAGAAT CATTGCCTCC ACCTGGGGAA 
ATCAAGCAAC TGACAGCTTG TGCCACATGG CAGTTTNTGT TCAGCACTGT GAAAGGCTGC 
TCTCTTTTTC AAATCCTGGT TTCTGTAAGA AACTGTTGAA AGTTTTTTGT GTACTTCCTA 
CAACCCACTC AAGGATGCAT GGGATATTGT GCAGAAGCTG TTTCTGAGGC AAATTTATAT 
TCATGTGGCC CTGATGAAAC TGAAATTGAA GGTGACTGTG TTTGTCAGCT GTCTGTCTCA 
TTACCACCAC CTTCAGGAAG GCCAGAGGTT GTGGTGGAGC TTATTGAGTC CAGGCTTTTC 
TGTAGGTGTG CTTTTGATGT TTTCCCTACA AACAACTTGG TGGGATTTCA CATAGCTTGG 
TCTAGGCTTT CTTCTCAAGA AATCAAAGAA GAGCTGAAAC AAGAGACCAC AGTTCAGGCA 
TTCTCTCTTT TAGAATTGGA TGGTATAAAT CTCAGACTTG GAGACAGGAT ATTTTGTAGC 
GCTTCTGTCT TTTTCTTGGA GAACCCACAT GTACAAAGTT TAGCCATCGA AAGCCGAGAA 
TTTTTTGCCG GCATTAAGGT CAGTTACAAC CTGAGCACTG TATCTGAAGA TGGGAATGAA 
CACCAGTTGA GGATAGAGAG CACAGTTCCT ATTGTTTGTC CTACTGGATT CAGTGAGCTT 
GATCAGGAGT GCAAAATCTC ATTAAAACTG ACAACTGTTG ATCAAGGTAC AGAACAGCTA 
GGCTTAAATT TGGCACTGTC TTCCCGTCAC GTGGACCTTT GTGGGACATC ATCCTGTGCT 
GACGGAACCT GCAGCCGTGC TTTTGTCTAC TATACTGCAG TTACACATTT TTCACAAGAT 
GGGAATAGAG TCACAAATAT TGTAGTAGAA CCTATAGTTC ATGAAAATTT CCTGTGGAAC 
AGCTATATTC CAGACAGCAT CCAGATCAGA GTGAAAGATG TCCCAACTGC TTACTGCTAT 
TCATTTGCTG ATCCACATAT AATCACCTTT GATAGCAGGA TATATGATAA CTTAAAGACT 
GGAACATTCG TGCTTTATAA GAGTATGTCA CGTGATTTTG AAGTCCATGT ACGTCAGTGG 
GACTGTGGAA GCTTTCACTA TCCTGTGTCA TGTAACTGCG GCTTTGTTGC CAAAGAAGAA 
GGGGATGTAG TTACTTTCGA TATATGCAGT GGTCAGCTAC ATGAATCACA GCCATATTTA 
TTTGCAAAGA GCCAAGATGT AACCAGCAAT ATCAAGATAA GTGAANTGTT AGGAAGAAAA 
GTCACAGTAT ACAAGGTTCC TGAGATTATA GAACTTGAGA ATGCTGGATT CTGTAATATT 
TGAAAATATA ACTGTGTGAT GGTGAGAGTT TTTGGTCAAG GCTTCAAAGA ATCACCTTCA 
ATTAAATGTG AAGTTACTAA ACTGAAGTTT AATAGCAGTA AATGGGTCCT TGAAGAAGCC 
CTCTACACAT ACACTGTTTT TCAAAACAGC AGGACTGTTG ATTGTCAGTT GCCCGCTGAT 
GTTCAGCAGT CTAGTACCAT AGATATGATG GGTGAGAAAC CAATCATGAA GTGGCAAATC 
AGTTCTAATG AGGTATCTAA CAATGGTTAT AGGTTCAGTA ATCCCAAAAT AATGATCATA 
TATGATGGAG CTTGTCAAGT TTGTGGTCTC TATGAAACGG ACTTGTGTAC TAGATCAGAT 
CAGTCGCTCA GTCGTGAAAA TGTTTGCATT ATCAATGGAC TCTGCTACAT TGAAGGGGAT 
AAAAATCCTA CCAGCCCTTG TTCAATTTGT AGACCTAAAA TTTCAAAATT TACATGGTCA 
TTTTTAGAAA GTAAGTTATT CTTTTTAATC CAAATATTTA AAAACAATTT AAAATCATGG 
AAGTTTAACT CTGATCTGTT TTTCGTGTAT ACATTTATAG TTGAAACATG CTTTTTAAAT 
GAATTTATTG CACATACTGT GGTTCTCACA AGGTCTGACA ACAGTGTAAC TAAGGAAGAG 
GATGGTATAT ATGCCNAGAG ACGGAGGAAT GTTAAGCCAA CGAATGGAAA TGCCTTTACC 
ATTTGTAAAC ATCCTTGGGG AAGAAACAGA GAGTGTGTTG CCACAAACAT ATGCAAATGC 
AAACCTGGTT ACACCGGTTC TGACTGCCAA ACTGATTAAC CATGAGCTTT ATTTTTTACA 
GCTGTATGTC ATCCTGACTG CAAAAACCAT GGAAAATGTA TTAAGCCTAA CATTTGTGAA 
TGTTCCCCAG GACATGGAAC AATCTGTGAT GAAGAAGGCT GTAGACCACC TTGTCAACAT 
GGTGGCACCT GCTTAGCTGG AAACCTCCAC TTTTGCCCGT ATGGTTTTGT AGGAATAAGG 
TGTGAAACAA TTGTCTGTAA CAGGCACTGT GAAAATGGAG GCAAATGTCT TAAACCAGAT 
ATCTGTGAGT GTAAGCCTGG CTGGTATGGA CCTACTTGTA GCACAGCTTT GTGTGACCCC 
ATTTGCCTCA ATGGTGGTTC CTGTAATAAA CCAAACACTT TCACCTGTCC AAATGGATTC 
TTTGGTACAC AGTGTCAGAA TGCTATCTGC CAGCCTCCCT GTAAGAATGG TGGTCACTGC 
ATGAGAAATA ATGTGTGCGT CTGTCCTGAA GGATACACTG GTAGGAGATG CCAAAAAAGT 
TTCTGTGTTC CCATGTGCAT TAATGGAGGA AAGTGTGTGG GACAAAGCCT TTGCTCTTGT 
CCTTCTGGCT GGACAGGGAA ACAGTGCAAC ACATCAATCT GCTTTCAGAA ATGTAAAAAT 
GGTGGTGAAT GTATTGCTCC CAGCATATGC CATCGTCCTA CCACACGGGC AGGAGCACAC 
TGTCAAACAC CAATTTGCAA TCAAAAGTGT CTTTATGGAG GCATATGTGT ACTCCCCAAC 
ATATGTTCTT GCCGCACTGG ATATTCTGGA GTCAAATGTG AAAAGAAAAT ACTGATGGAA 
TTAGCAGAGT TTCACCTGCA GATGGTGCTA GATCCTCAGT TCCCAGATAG CACACACGCA 
ATGCAAAACG ACCACTGGCT TATTACTGAT ACACCCAACA ACCTGTATGA ATCTAAGAAA 
ATGTCGAGTC AGACACAGGA GTATGTGATG TATGAGCCTA TTTACAGGAA GTTCAAAGAG 
AGACTAAAAT GGAAGCCGTG TACAAGAGGC CTCGCACCCA GCACTTTGGT CCAGAGTTCT 
CAGACGGCAA TGGCGACACC GGACACCTCA GTTCAAGATG GCCGCGGTGG GCTGTGCGAA 
GAGGTGCTGC GCCTGCGCTC CCGGGAGCCT CCAGCAGCCA ACAGCACGCC CGGGCCAATC 
GGCAAGAACT CCGCCCCTCC CTGCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006072563.1
CDS	1..3630
Translation	MKKIYKDIYLSNLAWECFPGGHQFLQSPYKNVHFDSLHLQSAIQDLICDHSLSPGWYLFLLFDRPAEMPTKCVEMNHCGSSAPIWLSLKDSESLPPPGEIKQLTACATWQFnullFSTVKGCSLFQILVSVRNCKFFVYFLQPTQGCMGYCAEAVSEANLYSCGPDETEIEGDCVCQLSVSLPPPSGRPEVVVELIESRLFCRCAFDVFPTNNLVGFHIAWSRLSSQEIKEELKQETTVQAFSLLELDGINLRLGDRIFCSASVFFLENPHVQSLAIESREFFAGIKVSYNLSTVSEDGNEHQLRIESTVPIVCPTGFSELDQECKISLKLTTVDQGTEQLGLNLALSSRHVDLCGTSSCADGTCSRAFVYYTAVTHFSQDGNRVTNIVVEPIVHENFLWNSYIPDSIQIRVKDVPTAYCYSFADPHIITFDSRIYDNLKTGTFVLYKSMSRDFEVHVRQWDCGSFHYPVSCNCGFVAKEEGDVVTFDICSGQLHESQPYLFAKSQDVTSNIKISEnullLGRKVTVYKVPEIIELENAGFCNIKYNCVMVRVFGQGFKESPSIKCEVTKLKFNSSKWVLEEALYTYTVFQNSRTVDCQLPADVQQSSTIDMMGEKPIMKWQISSNEVSNNGYRFSNPKIMIIYDGACQVCGLYETDLCTRSDQSLSRENVCIINGLCYIEGDKNPTSPCSICRPKISKFTWSFLESKLFFLIQIFKNNLKSWKFNSDLFFVYTFIVETCFLNEFIAHTVVLTRSDNSVTKEEDGIYAnullRRRNVKPTNGNAFTICKHPWGRNRECVATNICKCKPGYTGSDCQTDP*ALFFTAVCHPDCKNHGKCIKPNICECSPGHGTICDEEGCRPPCQHGGTCLAGNLHFCPYGFVGIRCETIVCNRHCENGGKCLKPDICECKPGWYGPTCSTALCDPICLNGGSCNKPNTFTCPNGFFGTQCQNAICQPPCKNGGHCMRNNVCVCPEGYTGRRCQKSFCVPMCINGGKCVGQSLCSCPSGWTGKQCNTSICFQKCKNGGECIAPSICHRPTTRAGAHCQTPICNQKCLYGGICVLPNICSCRTGYSGVKCEKKILMELAEFHLQMVLDPQFPDSTHAMQNDHWLITDTPNNLYESKKMSSQTQEYVMYEPIYRKFKERLKWKPCTRGLAPSTLVQSSQTAMATPDTSVQDGRGGLCEEVLRLRSREPPAANSTPGPIGKNSAPPCC

Target ORF information:

RefSeq Version	XM_006072501.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis von Willebrand factor D and EGF domains (VWDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006072501.1

ORF Insert Sequence:

ATGAAGAAAA TTTATAAAGA CATCTATCTT TCCAATCTTG CTTGGGAGTG CTTTCCTGGG 
GGACACCAGT TTCTTCAGAG CCCTTATAAA AATGTCCATT TTGACTCCTT GCACCTCCAA 
TAGTCAGCCA TTCAAGACCT AATATGTGAC CATTCCCTTT CTCCTGGATG GTATCTATTT 
CTGCTCTTTG ATAGACCTGC TGAGATGCCG ACCAAATGTG TTGAGATGAA CCACTGTGGT 
TCATCTGCCC CCATCTGGCT GTCTCTGAAA GATTCAGAAT CATTGCCTCC ACCTGGGGAA 
ATCAAGCAAC TGACAGCTTG TGCCACATGG CAGTTTNTGT TCAGCACTGT GAAAGGCTGC 
TCTCTTTTTC AAATCCTGGT TTCTGTAAGA AACTGTTGAA AGTTTTTTGT GTACTTCCTA 
CAACCCACTC AAGGATGCAT GGGATATTGT GCAGAAGCTG TTTCTGAGGC AAATTTATAT 
TCATGTGGCC CTGATGAAAC TGAAATTGAA GGTGACTGTG TTTGTCAGCT GTCTGTCTCA 
TTACCACCAC CTTCAGGAAG GCCAGAGGTT GTGGTGGAGC TTATTGAGTC CAGGCTTTTC 
TGTAGGTGTG CTTTTGATGT TTTCCCTACA AACAACTTGG TGGGATTTCA CATAGCTTGG 
TCTAGGCTTT CTTCTCAAGA AATCAAAGAA GAGCTGAAAC AAGAGACCAC AGTTCAGGCA 
TTCTCTCTTT TAGAATTGGA TGGTATAAAT CTCAGACTTG GAGACAGGAT ATTTTGTAGC 
GCTTCTGTCT TTTTCTTGGA GAACCCACAT GTACAAAGTT TAGCCATCGA AAGCCGAGAA 
TTTTTTGCCG GCATTAAGGT CAGTTACAAC CTGAGCACTG TATCTGAAGA TGGGAATGAA 
CACCAGTTGA GGATAGAGAG CACAGTTCCT ATTGTTTGTC CTACTGGATT CAGTGAGCTT 
GATCAGGAGT GCAAAATCTC ATTAAAACTG ACAACTGTTG ATCAAGGTAC AGAACAGCTA 
GGCTTAAATT TGGCACTGTC TTCCCGTCAC GTGGACCTTT GTGGGACATC ATCCTGTGCT 
GACGGAACCT GCAGCCGTGC TTTTGTCTAC TATACTGCAG TTACACATTT TTCACAAGAT 
GGGAATAGAG TCACAAATAT TGTAGTAGAA CCTATAGTTC ATGAAAATTT CCTGTGGAAC 
AGCTATATTC CAGACAGCAT CCAGATCAGA GTGAAAGATG TCCCAACTGC TTACTGCTAT 
TCATTTGCTG ATCCACATAT AATCACCTTT GATAGCAGGA TATATGATAA CTTAAAGACT 
GGAACATTCG TGCTTTATAA GAGTATGTCA CGTGATTTTG AAGTCCATGT ACGTCAGTGG 
GACTGTGGAA GCTTTCACTA TCCTGTGTCA TGTAACTGCG GCTTTGTTGC CAAAGAAGAA 
GGGGATGTAG TTACTTTCGA TATATGCAGT GGTCAGCTAC ATGAATCACA GCCATATTTA 
TTTGCAAAGA GCCAAGATGT AACCAGCAAT ATCAAGATAA GTGAANTGTT AGGAAGAAAA 
GTCACAGTAT ACAAGGTTCC TGAGATTATA GAACTTGAGA ATGCTGGATT CTGTAATATT 
TGAAAATATA ACTGTGTGAT GGTGAGAGTT TTTGGTCAAG GCTTCAAAGA ATCACCTTCA 
ATTAAATGTG AAGTTACTAA ACTGAAGTTT AATAGCAGTA AATGGGTCCT TGAAGAAGCC 
CTCTACACAT ACACTGTTTT TCAAAACAGC AGGACTGTTG ATTGTCAGTT GCCCGCTGAT 
GTTCAGCAGT CTAGTACCAT AGATATGATG GGTGAGAAAC CAATCATGAA GTGGCAAATC 
AGTTCTAATG AGGTATCTAA CAATGGTTAT AGGTTCAGTA ATCCCAAAAT AATGATCATA 
TATGATGGAG CTTGTCAAGT TTGTGGTCTC TATGAAACGG ACTTGTGTAC TAGATCAGAT 
CAGTCGCTCA GTCGTGAAAA TGTTTGCATT ATCAATGGAC TCTGCTACAT TGAAGGGGAT 
AAAAATCCTA CCAGCCCTTG TTCAATTTGT AGACCTAAAA TTTCAAAATT TACATGGTCA 
TTTTTAGAAA GTAAGTTATT CTTTTTAATC CAAATATTTA AAAACAATTT AAAATCATGG 
AAGTTTAACT CTGATCTGTT TTTCGTGTAT ACATTTATAG TTGAAACATG CTTTTTAAAT 
GAATTTATTG CACATACTGT GGTTCTCACA AGGTCTGACA ACAGTGTAAC TAAGGAAGAG 
GATGGTATAT ATGCCNAGAG ACGGAGGAAT GTTAAGCCAA CGAATGGAAA TGCCTTTACC 
ATTTGTAAAC ATCCTTGGGG AAGAAACAGA GAGTGTGTTG CCACAAACAT ATGCAAATGC 
AAACCTGGTT ACACCGGTTC TGACTGCCAA ACTGATTAAC CATGAGCTTT ATTTTTTACA 
GCTGTATGTC ATCCTGACTG CAAAAACCAT GGAAAATGTA TTAAGCCTAA CATTTGTGAA 
TGTTCCCCAG GACATGGAAC AATCTGTGAT GAAGAAGGCT GTAGACCACC TTGTCAACAT 
GGTGGCACCT GCTTAGCTGG AAACCTCCAC TTTTGCCCGT ATGGTTTTGT AGGAATAAGG 
TGTGAAACAA TTGTCTGTAA CAGGCACTGT GAAAATGGAG GCAAATGTCT TAAACCAGAT 
ATCTGTGAGT GTAAGCCTGG CTGGTATGGA CCTACTTGTA GCACAGCTTT GTGTGACCCC 
ATTTGCCTCA ATGGTGGTTC CTGTAATAAA CCAAACACTT TCACCTGTCC AAATGGATTC 
TTTGGTACAC AGTGTCAGAA TGCTATCTGC CAGCCTCCCT GTAAGAATGG TGGTCACTGC 
ATGAGAAATA ATGTGTGCGT CTGTCCTGAA GGATACACTG GTAGGAGATG CCAAAAAAGT 
TTCTGTGTTC CCATGTGCAT TAATGGAGGA AAGTGTGTGG GACAAAGCCT TTGCTCTTGT 
CCTTCTGGCT GGACAGGGAA ACAGTGCAAC ACATCAATCT GCTTTCAGAA ATGTAAAAAT 
GGTGGTGAAT GTATTGCTCC CAGCATATGC CATCGTCCTA CCACACGGGC AGGAGCACAC 
TGTCAAACAC CAATTTGCAA TCAAAAGTGT CTTTATGGAG GCATATGTGT ACTCCCCAAC 
ATATGTTCTT GCCGCACTGG ATATTCTGGA GTCAAATGTG AAAAGAAAAT ACTGATGGAA 
TTAGCAGAGT TTCACCTGCA GATGGTGCTA GATCCTCAGT TCCCAGATAG CACACACGCA 
ATGCAAAACG ACCACTGGCT TATTACTGAT ACACCCAACA ACCTGTATGA ATCTAAGAAA 
ATGTCGAGTC AGACACAGGA GTATGTGATG TATGAGCCTA TTTACAGGAA GTTCAAAGAG 
AGACTAAAAT GGAAGCCGTG TACAAGAGGC CTCGCACCCA GCACTTTGGT CCAGAGTTCT 
CAGACGGCAA TGGCGACACC GGACACCTCA GTTCAAGATG GCCGCGGTGG GCTGTGCGAA 
GAGGTGCTGC GCCTGCGCTC CCGGGAGCCT CCAGCAGCCA ACAGCACGCC CGGGCCAATC 
GGCAAGAACT CCGCCCCTCC CTGCTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VWDE ( XM_025291968.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025147753.1 Bubalus bubalis von Willebrand factor D and EGF domains (VWDE), mRNA.

Price & Availability↑

CloneID	OBu71343
Clone ID Related Accession (Same CDS sequence)	XM_025291968.1
Accession Version	XM_025291968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	LOW QUALITY PROTEIN: von Willebrand factor D and EGF domain-containing protein
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037552.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases frameshifts :: corrected 4 indels internal stop codons :: corrected 5 genomic stop codons ##RefSeq-Attributes-END##

ATGTCAATGA AGAAAATTTA TAAAGACATC TATCTTTCCA ATCTTGCTTG GGAGTGCTTT 
CCTGGGGGAC ACCAGTTTCT TCAGAGCCCT TATAAAAATG TCCATTTTGA CTCCTTGCAC 
CTCCAATAGT CAGCCATTCA AGACCTAATA TGTGACCATT CCCTTTCTCC TGGATGGTAT 
CTATTTCTGC TCTTTGATAG ACCTGCTGAG ATGCCGACCA AATGTGTTGA GATGAACCAC 
TGTGGTTCAT CTGCCCCCAT CTGGCTGTCT CTGAAAGATT CAGAATCATT GCCTCCACCT 
GGGGAAATCA AGCAACTGAC AGCTTGTGCC ACATGGCAGT TTNTGTTCAG CACTGTGAAA 
GGCTGCTCTC TTTTTCAAAT CCTGGTTTCT GTAAGAAACT GTTGAAAGTT TTTTGTGTAC 
TTCCTACAAC CCACTCAAGG ATGCATGGGA TATTGTGCAG AAGCTGTTTC TGAGGCAAAT 
TTATATTCAT GTGGCCCTGA TGAAACTGAA ATTGAAGGTG ACTGTGTTTG TCAGCTGTCT 
GTCTCATTAC CACCACCTTC AGGAAGGCCA GAGGTTGTGG TGGAGCTTAT TGAGTCCAGG 
CTTTTCTGTA GGTGTGCTTT TGATGTTTTC CCTACAAACA ACTTGGTGGG ATTTCACATA 
GCTTGGTCTA GGCTTTCTTC TCAAGAAATC AAAGAAGAGC TGAAACAAGA GACCACAGTT 
CAGGCATTCT CTCTTTTAGA ATTGGATGGT ATAAATCTCA GACTTGGAGA CAGGATATTT 
TGTAGCGCTT CTGTCTTTTT CTTGGAGAAC CCACATGTAC AAAGTTTAGC CATCGAAAGC 
CGAGAATTTT TTGCCGGCAT TAAGGTCAGT TACAACCTGA GCACTGTATC TGAAGATGGG 
AATGAACACC AGTTGAGGAT AGAGAGCACA GTTCCTATTG TTTGTCCTAC TGGATTCAGT 
GAGCTTGATC AGGAGTGCAA AATCTCATTA AAACTGACAA CTGTTGATCA AGGTACAGAA 
CAGCTAGGCT TAAATTTGGC ACTGTCTTCC CGTCACGTGG ACCTTTGTGG GACATCATCC 
TGTGCTGACG GAACCTGCAG CCGTGCTTTT GTCTACTATA CTGCAGTTAC ACATTTTTCA 
CAAGATGGGA ATAGAGTCAC AAATATTGTA GTAGAACCTA TAGTTCATGA AAATTTCCTG 
TGGAACAGCT ATATTCCAGA CAGCATCCAG ATCAGAGTGA AAGATGTCCC AACTGCTTAC 
TGCTATTCAT TTGCTGATCC ACATATAATC ACCTTTGATA GCAGGATATA TGATAACTTA 
AAGACTGGAA CATTCGTGCT TTATAAGAGT ATGTCACGTG ATTTTGAAGT CCATGTACGT 
CAGTGGGACT GTGGAAGCTT TCACTATCCT GTGTCATGTA ACTGCGGCTT TGTTGCCAAA 
GAAGAAGGGG ATGTAGTTAC TTTCGATATA TGCAGTGGTC AGCTACATGA ATCACAGCCA 
TATTTATTTG CAAAGAGCCA AGATGTAACC AGCAATATCA AGATAAGTGA ANTGTTAGGA 
AGAAAAGTCA CAGTATACAA GGTTCCTGAG ATTATAGAAC TTGAGAATGC TGGATTCTGT 
AATATTTGAA AATATAACTG TGTGATGGTG AGAGTTTTTG GTCAAGGCTT CAAAGAATCA 
CCTTCAATTA AATGTGAAGT TACTAAACTG AAGTTTAATA GCAGTAAATG GGTCCTTGAA 
GAAGCCCTCT ACACATACAC TGTTTTTCAA AACAGCAGGA CTGTTGATTG TCAGTTGCCC 
GCTGATGTTC AGCAGTCTAG TACCATAGAT ATGATGGGTG AGAAACCAAT CATGAAGTGG 
CAAATCAGTT CTAATGAGGT ATCTAACAAT GGTTATAGGT TCAGTAATCC CAAAATAATG 
ATCATATATG ATGGAGCTTG TCAAGTTTGT GGTCTCTATG AAACGGACTT GTGTACTAGA 
TCAGATCAGT CGCTCAGTCG TGAAAATGTT TGCATTATCA ATGGACTCTG CTACATTGAA 
GGGGATAAAA ATCCTACCAG CCCTTGTTCA ATTTGTAGAC CTAAAATTTC AAAATTTACA 
TGGTCATTTT TAGAAAGTAA GTTATTCTTT TTAATCCAAA TATTTAAAAA CAATTTAAAA 
TCATGGTATA TGTTCTTATT CACAGTGCTT TGGTTTCAGA AGTTTAACTC TGATCTGTTT 
TTCGTGTATA CATTTATAGT TGAAACATGC TTTTTAAATG AATTTATTGC ACATACTGTG 
GTTCTCACAA GGTCTGACAA CAGTGTAACT AAGGAAGAGG ATGGTATATA TGCCNAGAGA 
CGGAGGAATG TTAAGCCAAC GAATGGAAAT GCCTTTACCA TTTGTAAACA TCCTTGGGGA 
AGAAACAGAG AGTGTGTTGC CACAAACATA TGCAAATGCA AACCTGGTTA CACCGGTTCT 
GACTGCCAAA CTGATTAACC ATGAGCTTTA TTTTTTACAG CTGTATGTCA TCCTGACTGC 
AAAAACCATG GAAAATGTAT TAAGCCTAAC ATTTGTGAAT GTTCCCCAGG ACATGGAACA 
ATCTGTGATG AAGAAGGCTG TAGACCACCT TGTCAACATG GTGGCACCTG CTTAGCTGGA 
AACCTCCACT TTTGCCCGTA TGGTTTTGTA GGAATAAGGT GTGAAACAAT TGTCTGTAAC 
AGGCACTGTG AAAATGGAGG CAAATGTCTT AAACCAGATA TCTGTGAGTG TAAGCCTGGC 
TGGTATGGAC CTACTTGTAG CACAGCTTTG TGTGACCCCA TTTGCCTCAA TGGTGGTTCC 
TGTAATAAAC CAAACACTTT CACCTGTCCA AATGGATTCT TTGGTACACA GTGTCAGAAT 
GCTATCTGCC AGCCTCCCTG TAAGAATGGT GGTCACTGCA TGAGAAATAA TGTGTGCGTC 
TGTCCTGAAG GATACACTGG TAGGAGATGC CAAAAAAGTT TCTGTGTTCC CATGTGCATT 
AATGGAGGAA AGTGTGTGGG ACAAAGCCTT TGCTCTTGTC CTTCTGGCTG GACAGGGAAA 
CAGTGCAACA CATCAATCTG CTTTCAGAAA TGTAAAAATG GTGGTGAATG TATTGCTCCC 
AGCATATGCC ATCGTCCTAC CACACGGGCA GGAGCACACT GTCAAACACC AATTTGCAAT 
CAAAAGTGTC TTTATGGAGG CATATGTGTA CTCCCCAACA TATGTTCTTG CCGCACTGGA 
TATTCTGGAG TCAAATGTGA AAAGAAAATA CTGGTATTTT ACAGTTTAAA CAAAGATTGT 
ATTAGATATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025147753.1
CDS	280..3591
Translation	MSMKKIYKDIYLSNLAWECFPGGHQFLQSPYKNVHFDSLHLQSAIQDLICDHSLSPGWYLFLLFDRPAEMPTKCVEMNHCGSSAPIWLSLKDSESLPPPGEIKQLTACATWQFnullFSTVKGCSLFQILVSVRNCKFFVYFLQPTQGCMGYCAEAVSEANLYSCGPDETEIEGDCVCQLSVSLPPPSGRPEVVVELIESRLFCRCAFDVFPTNNLVGFHIAWSRLSSQEIKEELKQETTVQAFSLLELDGINLRLGDRIFCSASVFFLENPHVQSLAIESREFFAGIKVSYNLSTVSEDGNEHQLRIESTVPIVCPTGFSELDQECKISLKLTTVDQGTEQLGLNLALSSRHVDLCGTSSCADGTCSRAFVYYTAVTHFSQDGNRVTNIVVEPIVHENFLWNSYIPDSIQIRVKDVPTAYCYSFADPHIITFDSRIYDNLKTGTFVLYKSMSRDFEVHVRQWDCGSFHYPVSCNCGFVAKEEGDVVTFDICSGQLHESQPYLFAKSQDVTSNIKISEnullLGRKVTVYKVPEIIELENAGFCNIKYNCVMVRVFGQGFKESPSIKCEVTKLKFNSSKWVLEEALYTYTVFQNSRTVDCQLPADVQQSSTIDMMGEKPIMKWQISSNEVSNNGYRFSNPKIMIIYDGACQVCGLYETDLCTRSDQSLSRENVCIINGLCYIEGDKNPTSPCSICRPKISKFTWSFLESKLFFLIQIFKNNLKSWYMFLFTVLWFQKFNSDLFFVYTFIVETCFLNEFIAHTVVLTRSDNSVTKEEDGIYAnullRRRNVKPTNGNAFTICKHPWGRNRECVATNICKCKPGYTGSDCQTDP*ALFFTAVCHPDCKNHGKCIKPNICECSPGHGTICDEEGCRPPCQHGGTCLAGNLHFCPYGFVGIRCETIVCNRHCENGGKCLKPDICECKPGWYGPTCSTALCDPICLNGGSCNKPNTFTCPNGFFGTQCQNAICQPPCKNGGHCMRNNVCVCPEGYTGRRCQKSFCVPMCINGGKCVGQSLCSCPSGWTGKQCNTSICFQKCKNGGECIAPSICHRPTTRAGAHCQTPICNQKCLYGGICVLPNICSCRTGYSGVKCEKKILVFYSLNKDCIRY

Target ORF information:

RefSeq Version	XM_025291968.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis von Willebrand factor D and EGF domains (VWDE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025291968.1

ORF Insert Sequence:

ATGTCAATGA AGAAAATTTA TAAAGACATC TATCTTTCCA ATCTTGCTTG GGAGTGCTTT 
CCTGGGGGAC ACCAGTTTCT TCAGAGCCCT TATAAAAATG TCCATTTTGA CTCCTTGCAC 
CTCCAATAGT CAGCCATTCA AGACCTAATA TGTGACCATT CCCTTTCTCC TGGATGGTAT 
CTATTTCTGC TCTTTGATAG ACCTGCTGAG ATGCCGACCA AATGTGTTGA GATGAACCAC 
TGTGGTTCAT CTGCCCCCAT CTGGCTGTCT CTGAAAGATT CAGAATCATT GCCTCCACCT 
GGGGAAATCA AGCAACTGAC AGCTTGTGCC ACATGGCAGT TTNTGTTCAG CACTGTGAAA 
GGCTGCTCTC TTTTTCAAAT CCTGGTTTCT GTAAGAAACT GTTGAAAGTT TTTTGTGTAC 
TTCCTACAAC CCACTCAAGG ATGCATGGGA TATTGTGCAG AAGCTGTTTC TGAGGCAAAT 
TTATATTCAT GTGGCCCTGA TGAAACTGAA ATTGAAGGTG ACTGTGTTTG TCAGCTGTCT 
GTCTCATTAC CACCACCTTC AGGAAGGCCA GAGGTTGTGG TGGAGCTTAT TGAGTCCAGG 
CTTTTCTGTA GGTGTGCTTT TGATGTTTTC CCTACAAACA ACTTGGTGGG ATTTCACATA 
GCTTGGTCTA GGCTTTCTTC TCAAGAAATC AAAGAAGAGC TGAAACAAGA GACCACAGTT 
CAGGCATTCT CTCTTTTAGA ATTGGATGGT ATAAATCTCA GACTTGGAGA CAGGATATTT 
TGTAGCGCTT CTGTCTTTTT CTTGGAGAAC CCACATGTAC AAAGTTTAGC CATCGAAAGC 
CGAGAATTTT TTGCCGGCAT TAAGGTCAGT TACAACCTGA GCACTGTATC TGAAGATGGG 
AATGAACACC AGTTGAGGAT AGAGAGCACA GTTCCTATTG TTTGTCCTAC TGGATTCAGT 
GAGCTTGATC AGGAGTGCAA AATCTCATTA AAACTGACAA CTGTTGATCA AGGTACAGAA 
CAGCTAGGCT TAAATTTGGC ACTGTCTTCC CGTCACGTGG ACCTTTGTGG GACATCATCC 
TGTGCTGACG GAACCTGCAG CCGTGCTTTT GTCTACTATA CTGCAGTTAC ACATTTTTCA 
CAAGATGGGA ATAGAGTCAC AAATATTGTA GTAGAACCTA TAGTTCATGA AAATTTCCTG 
TGGAACAGCT ATATTCCAGA CAGCATCCAG ATCAGAGTGA AAGATGTCCC AACTGCTTAC 
TGCTATTCAT TTGCTGATCC ACATATAATC ACCTTTGATA GCAGGATATA TGATAACTTA 
AAGACTGGAA CATTCGTGCT TTATAAGAGT ATGTCACGTG ATTTTGAAGT CCATGTACGT 
CAGTGGGACT GTGGAAGCTT TCACTATCCT GTGTCATGTA ACTGCGGCTT TGTTGCCAAA 
GAAGAAGGGG ATGTAGTTAC TTTCGATATA TGCAGTGGTC AGCTACATGA ATCACAGCCA 
TATTTATTTG CAAAGAGCCA AGATGTAACC AGCAATATCA AGATAAGTGA ANTGTTAGGA 
AGAAAAGTCA CAGTATACAA GGTTCCTGAG ATTATAGAAC TTGAGAATGC TGGATTCTGT 
AATATTTGAA AATATAACTG TGTGATGGTG AGAGTTTTTG GTCAAGGCTT CAAAGAATCA 
CCTTCAATTA AATGTGAAGT TACTAAACTG AAGTTTAATA GCAGTAAATG GGTCCTTGAA 
GAAGCCCTCT ACACATACAC TGTTTTTCAA AACAGCAGGA CTGTTGATTG TCAGTTGCCC 
GCTGATGTTC AGCAGTCTAG TACCATAGAT ATGATGGGTG AGAAACCAAT CATGAAGTGG 
CAAATCAGTT CTAATGAGGT ATCTAACAAT GGTTATAGGT TCAGTAATCC CAAAATAATG 
ATCATATATG ATGGAGCTTG TCAAGTTTGT GGTCTCTATG AAACGGACTT GTGTACTAGA 
TCAGATCAGT CGCTCAGTCG TGAAAATGTT TGCATTATCA ATGGACTCTG CTACATTGAA 
GGGGATAAAA ATCCTACCAG CCCTTGTTCA ATTTGTAGAC CTAAAATTTC AAAATTTACA 
TGGTCATTTT TAGAAAGTAA GTTATTCTTT TTAATCCAAA TATTTAAAAA CAATTTAAAA 
TCATGGTATA TGTTCTTATT CACAGTGCTT TGGTTTCAGA AGTTTAACTC TGATCTGTTT 
TTCGTGTATA CATTTATAGT TGAAACATGC TTTTTAAATG AATTTATTGC ACATACTGTG 
GTTCTCACAA GGTCTGACAA CAGTGTAACT AAGGAAGAGG ATGGTATATA TGCCNAGAGA 
CGGAGGAATG TTAAGCCAAC GAATGGAAAT GCCTTTACCA TTTGTAAACA TCCTTGGGGA 
AGAAACAGAG AGTGTGTTGC CACAAACATA TGCAAATGCA AACCTGGTTA CACCGGTTCT 
GACTGCCAAA CTGATTAACC ATGAGCTTTA TTTTTTACAG CTGTATGTCA TCCTGACTGC 
AAAAACCATG GAAAATGTAT TAAGCCTAAC ATTTGTGAAT GTTCCCCAGG ACATGGAACA 
ATCTGTGATG AAGAAGGCTG TAGACCACCT TGTCAACATG GTGGCACCTG CTTAGCTGGA 
AACCTCCACT TTTGCCCGTA TGGTTTTGTA GGAATAAGGT GTGAAACAAT TGTCTGTAAC 
AGGCACTGTG AAAATGGAGG CAAATGTCTT AAACCAGATA TCTGTGAGTG TAAGCCTGGC 
TGGTATGGAC CTACTTGTAG CACAGCTTTG TGTGACCCCA TTTGCCTCAA TGGTGGTTCC 
TGTAATAAAC CAAACACTTT CACCTGTCCA AATGGATTCT TTGGTACACA GTGTCAGAAT 
GCTATCTGCC AGCCTCCCTG TAAGAATGGT GGTCACTGCA TGAGAAATAA TGTGTGCGTC 
TGTCCTGAAG GATACACTGG TAGGAGATGC CAAAAAAGTT TCTGTGTTCC CATGTGCATT 
AATGGAGGAA AGTGTGTGGG ACAAAGCCTT TGCTCTTGTC CTTCTGGCTG GACAGGGAAA 
CAGTGCAACA CATCAATCTG CTTTCAGAAA TGTAAAAATG GTGGTGAATG TATTGCTCCC 
AGCATATGCC ATCGTCCTAC CACACGGGCA GGAGCACACT GTCAAACACC AATTTGCAAT 
CAAAAGTGTC TTTATGGAGG CATATGTGTA CTCCCCAACA TATGTTCTTG CCGCACTGGA 
TATTCTGGAG TCAAATGTGA AAAGAAAATA CTGGTATTTT ACAGTTTAAA CAAAGATTGT 
ATTAGATATT GA