VARS2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	VARS2
Entrez Gene ID	102400298
Full Name	valyl-tRNA synthetase 2, mitochondrial
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006052313.1	XP_006052375.1	valine--tRNA ligase, mitochondrial isoform X2
XM_006052312.2	XP_006052374.2	valine--tRNA ligase, mitochondrial isoform X1
XM_006052313.2	XP_006052375.2	valine--tRNA ligase, mitochondrial isoform X1
XM_025296492.1	XP_025152277.1	valine--tRNA ligase, mitochondrial isoform X2
XM_006052312.1	XP_006052374.1	valine--tRNA ligase, mitochondrial isoform X1

Pathways from BioSystems
Gene Ontology

The following VARS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VARS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu10039	XM_006052312.1 Latest version!	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu10039	XM_006052313.1 Latest version!	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu54227	XM_006052313.2 Latest version!	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu54227	XM_006052312.2 Latest version!	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBu54228	XM_025296492.1 Latest version!	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VARS2 ( XM_006052313.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052375.1 Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu10039
Clone ID Related Accession (Same CDS sequence)	XM_006052313.1 , XM_006052312.1
Accession Version	XM_006052313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	valine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784454.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCATCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CAACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGGGTGATAC GGAGCACTCC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC 
AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC 
CCTGATGTCC TGGACACATG GTTCTCCTCG GCTCTTTTCC CCTTTTCTGC CCTAGGCTGG 
CCCCGAGAGA CCCTGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT 
GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG 
CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG 
GTACTGCAGG ACAAGCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC 
TCGGCACAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG 
TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGCGACTTGC ACCTGTCCGT CTCTGAGGTC 
CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT 
CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC 
GCCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAAGACT GCGAGCGGGG CTTCCTCGCC 
CGCGAGCTCT CGCTCGTCAC CCATGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT 
GTCTACTTGG AGGCTGTGAA GCCGCTGCTG TCACACGCTC CCCGCCCTCT GGAGCCGCCT 
CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC 
CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC 
ATCTGTGTTG CCCCCTACCC GAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG 
GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGCGC TCAGGGCTCT GCGCGCCACC 
TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG 
GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCCACTGTGG GGTGGTGGGC 
CTTTTACCCC CTGGTGCAGC AGCCCCCTCG GGCTGGGTCC AGGCCGCCCT CAGTGACACC 
AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG 
GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCA GCCCCCATCC 
GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA 
AAGCTGGACA AGGCAGCCTC TCACCTCCGG CAGCTGGTGG ATGCGTCTCC AAGCCCTGGG 
GGCTCACCAT CCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052375.1
CDS	56..3253
Translation	MPHLPLTSFRPPLWGLRLSRGLPRSRPLSTQSEPDGSAISRRNREAKQKRLREKQASLEAGIAPKSKSPAESSKAWTPKERVLYEISTEHGEKKDVSRPLPPAYSPQYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREDFLREVWKWKEEKGGEICEQLRALGASLDWDRECFTMDTGSSMAVTEAFVRLYKAGLLYRSRQLVNWSCALRSAISDIEVESRPLPGRTELQLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLCHPLTGQLLPLITDCTVQPRLGTGAVKVTPAHSPADAELGARHGLSPRSVIAEDGTMTALCEDWLQGLHRFVAREKILSALRERGLFRGLQNHPMVLPICSRSGDVVEYLLKSQWFVRCREMGERAAKAVESGALDLSPSFHQKNWQHWFSHIGDWCVSRQLWWGHRIPAYLVVEEHAKGDTEHSWVVGRTEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPRETLDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGVELQVLQDKLRDGNLDPAELAIAASAQRKDFPHGIPECGTDALRFTLCSHGALGGDLHLSVSEVLSFRHFCNKIWNALRFILNALGEEFIPQPAEELSPASRMDAWILSCLARTAQDCERGFLARELSLVTHALHHFWLHNLCDVYLEAVKPLLSHAPRPLEPPQVLFFCADVGLRLLAPLMPFLAEELWQRLPPRPGGPSAPSICVAPYPSARSLEHWHQPELERRFSRVQESVQALRALRATYQLTKARPRVLLQSSEPGEQGLFEAFLEPLGILGHCGVVGLLPPGAAAPSGWVQAALSDTSQAYMELQGLVDPQTHLPRLAARRHKLQKQLDGLLAQPPSEGQAETQRQQRLSSLQLELSKLDKAASHLRQLVDASPSPGGSPSP

Target ORF information:

RefSeq Version	XM_006052313.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052313.1

ORF Insert Sequence:

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCATCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CAACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGGGTGATAC GGAGCACTCC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC 
AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC 
CCTGATGTCC TGGACACATG GTTCTCCTCG GCTCTTTTCC CCTTTTCTGC CCTAGGCTGG 
CCCCGAGAGA CCCTGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT 
GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG 
CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG 
GTACTGCAGG ACAAGCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC 
TCGGCACAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG 
TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGCGACTTGC ACCTGTCCGT CTCTGAGGTC 
CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT 
CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC 
GCCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAAGACT GCGAGCGGGG CTTCCTCGCC 
CGCGAGCTCT CGCTCGTCAC CCATGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT 
GTCTACTTGG AGGCTGTGAA GCCGCTGCTG TCACACGCTC CCCGCCCTCT GGAGCCGCCT 
CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC 
CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC 
ATCTGTGTTG CCCCCTACCC GAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG 
GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGCGC TCAGGGCTCT GCGCGCCACC 
TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG 
GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCCACTGTGG GGTGGTGGGC 
CTTTTACCCC CTGGTGCAGC AGCCCCCTCG GGCTGGGTCC AGGCCGCCCT CAGTGACACC 
AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG 
GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCA GCCCCCATCC 
GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA 
AAGCTGGACA AGGCAGCCTC TCACCTCCGG CAGCTGGTGG ATGCGTCTCC AAGCCCTGGG 
GGCTCACCAT CCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_006052312.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052374.1 Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu10039
Clone ID Related Accession (Same CDS sequence)	XM_006052313.1 , XM_006052312.1
Accession Version	XM_006052312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	valine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005784454.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCATCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CAACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGGGTGATAC GGAGCACTCC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC 
AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC 
CCTGATGTCC TGGACACATG GTTCTCCTCG GCTCTTTTCC CCTTTTCTGC CCTAGGCTGG 
CCCCGAGAGA CCCTGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT 
GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG 
CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG 
GTACTGCAGG ACAAGCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC 
TCGGCACAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG 
TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGCGACTTGC ACCTGTCCGT CTCTGAGGTC 
CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT 
CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC 
GCCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAAGACT GCGAGCGGGG CTTCCTCGCC 
CGCGAGCTCT CGCTCGTCAC CCATGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT 
GTCTACTTGG AGGCTGTGAA GCCGCTGCTG TCACACGCTC CCCGCCCTCT GGAGCCGCCT 
CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC 
CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC 
ATCTGTGTTG CCCCCTACCC GAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG 
GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGCGC TCAGGGCTCT GCGCGCCACC 
TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG 
GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCCACTGTGG GGTGGTGGGC 
CTTTTACCCC CTGGTGCAGC AGCCCCCTCG GGCTGGGTCC AGGCCGCCCT CAGTGACACC 
AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG 
GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCA GCCCCCATCC 
GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA 
AAGCTGGACA AGGCAGCCTC TCACCTCCGG CAGCTGGTGG ATGCGTCTCC AAGCCCTGGG 
GGCTCACCAT CCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052374.1
CDS	162..3359
Translation	MPHLPLTSFRPPLWGLRLSRGLPRSRPLSTQSEPDGSAISRRNREAKQKRLREKQASLEAGIAPKSKSPAESSKAWTPKERVLYEISTEHGEKKDVSRPLPPAYSPQYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREDFLREVWKWKEEKGGEICEQLRALGASLDWDRECFTMDTGSSMAVTEAFVRLYKAGLLYRSRQLVNWSCALRSAISDIEVESRPLPGRTELQLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLCHPLTGQLLPLITDCTVQPRLGTGAVKVTPAHSPADAELGARHGLSPRSVIAEDGTMTALCEDWLQGLHRFVAREKILSALRERGLFRGLQNHPMVLPICSRSGDVVEYLLKSQWFVRCREMGERAAKAVESGALDLSPSFHQKNWQHWFSHIGDWCVSRQLWWGHRIPAYLVVEEHAKGDTEHSWVVGRTEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPRETLDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGVELQVLQDKLRDGNLDPAELAIAASAQRKDFPHGIPECGTDALRFTLCSHGALGGDLHLSVSEVLSFRHFCNKIWNALRFILNALGEEFIPQPAEELSPASRMDAWILSCLARTAQDCERGFLARELSLVTHALHHFWLHNLCDVYLEAVKPLLSHAPRPLEPPQVLFFCADVGLRLLAPLMPFLAEELWQRLPPRPGGPSAPSICVAPYPSARSLEHWHQPELERRFSRVQESVQALRALRATYQLTKARPRVLLQSSEPGEQGLFEAFLEPLGILGHCGVVGLLPPGAAAPSGWVQAALSDTSQAYMELQGLVDPQTHLPRLAARRHKLQKQLDGLLAQPPSEGQAETQRQQRLSSLQLELSKLDKAASHLRQLVDASPSPGGSPSP

Target ORF information:

RefSeq Version	XM_006052312.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052312.1

ORF Insert Sequence:

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCATCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CAACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGGGTGATAC GGAGCACTCC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC 
AGAGAAGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC 
CCTGATGTCC TGGACACATG GTTCTCCTCG GCTCTTTTCC CCTTTTCTGC CCTAGGCTGG 
CCCCGAGAGA CCCTGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT 
GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG 
CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG 
GTACTGCAGG ACAAGCTGAG GGATGGAAAC TTGGACCCCG CAGAGCTGGC GATTGCAGCC 
TCGGCACAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG 
TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGCGACTTGC ACCTGTCCGT CTCTGAGGTC 
CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT 
CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC 
GCCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAAGACT GCGAGCGGGG CTTCCTCGCC 
CGCGAGCTCT CGCTCGTCAC CCATGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT 
GTCTACTTGG AGGCTGTGAA GCCGCTGCTG TCACACGCTC CCCGCCCTCT GGAGCCGCCT 
CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC 
CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC 
ATCTGTGTTG CCCCCTACCC GAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG 
GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGCGC TCAGGGCTCT GCGCGCCACC 
TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG 
GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCCACTGTGG GGTGGTGGGC 
CTTTTACCCC CTGGTGCAGC AGCCCCCTCG GGCTGGGTCC AGGCCGCCCT CAGTGACACC 
AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG 
GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCA GCCCCCATCC 
GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA 
AAGCTGGACA AGGCAGCCTC TCACCTCCGG CAGCTGGTGG ATGCGTCTCC AAGCCCTGGG 
GGCTCACCAT CCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_006052312.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052374.2 Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBu54227
Clone ID Related Accession (Same CDS sequence)	XM_006052312.2 , XM_006052313.2
Accession Version	XM_006052312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	valine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052312.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCGTCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGGGTGATAC GGAGCACTCC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC 
AGAGAGGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC 
CCTGATGTCC TGGACACATG GTTCTCCTCG GCTCTTTTCC CCTTTTCTGC CCTAGGCTGG 
CCCCGAGAGA CCCTGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT 
GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG 
CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG 
GTACTGCAGG ACAAGCTGAG GGATGGAAAC TTGGACCCCA CAGAGCTGGC GATTGCAGCC 
TCGGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG 
TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGCGACTTGC ACCTGTCCGT CTCTGAGGTC 
CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT 
CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC 
GCCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCGCC 
CGCGAGCTCT CGCTCGTCAC CCATGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT 
GTCTACTTGG AGGCTGTGAA GCCGCTGCTG TCACACGCTC CCCGCCCTCT GGAGCCGCCT 
CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC 
CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC 
ATCTGTGTTG CCCCCTACCC GAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG 
GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGCGC TCAGGGCTCT GCGCGCCACC 
TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG 
GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCCACTGTGG GGTGGTGGGC 
CTTTTACCCC CTGGTGCAGC AGCCCCCTCG GGCTGGGTCC AGGCCGCCCT CAGTGACACC 
AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG 
GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCA GCCCCCATCC 
GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA 
AAGCTGGACA AGGCAGCCTC TCACCTCCGG CAGCTGGTGG ATGCGTCTCC AAGCCCTGGG 
GGCTCACCAT CCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052374.2
CDS	170..3367
Translation	MPHLPLTSFRPPLWGLRLSRGLPRSRPLSTQSEPDGSAVSRRNREAKQKRLREKQASLEAGIAPKSKSPAESSKAWTPKERVLYEISTEHGEKKDVSRPLPPAYSPQYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREDFLREVWKWKEEKGGEICEQLRALGASLDWDRECFTMDTGSSMAVTEAFVRLYKAGLLYRSRQLVNWSCALRSAISDIEVESRPLPGRTELQLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLCHPLTGQLLPLITDCTVQPRLGTGAVKVTPAHSPADAELGARHGLSPRSVIAEDGTMTALCEDWLQGLHRFVAREKILSALRERGLFRGLQNHPMVLPICSRSGDVVEYLLKSQWFVRCREMGERAAKAVESGALDLSPSFHQKNWQHWFSHIGDWCVSRQLWWGHRIPAYLVVEEHAKGDTEHSWVVGRTEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPRETLDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGVELQVLQDKLRDGNLDPTELAIAASAQRKDFPHGIPECGTDALRFTLCSHGALGGDLHLSVSEVLSFRHFCNKIWNALRFILNALGEEFIPQPAEELSPASRMDAWILSCLARTAQDCERGFLARELSLVTHALHHFWLHNLCDVYLEAVKPLLSHAPRPLEPPQVLFFCADVGLRLLAPLMPFLAEELWQRLPPRPGGPSAPSICVAPYPSARSLEHWHQPELERRFSRVQESVQALRALRATYQLTKARPRVLLQSSEPGEQGLFEAFLEPLGILGHCGVVGLLPPGAAAPSGWVQAALSDTSQAYMELQGLVDPQTHLPRLAARRHKLQKQLDGLLAQPPSEGQAETQRQQRLSSLQLELSKLDKAASHLRQLVDASPSPGGSPSP

Target ORF information:

RefSeq Version	XM_006052312.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052312.2

ORF Insert Sequence:

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCGTCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGGGTGATAC GGAGCACTCC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC 
AGAGAGGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC 
CCTGATGTCC TGGACACATG GTTCTCCTCG GCTCTTTTCC CCTTTTCTGC CCTAGGCTGG 
CCCCGAGAGA CCCTGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT 
GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG 
CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG 
GTACTGCAGG ACAAGCTGAG GGATGGAAAC TTGGACCCCA CAGAGCTGGC GATTGCAGCC 
TCGGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG 
TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGCGACTTGC ACCTGTCCGT CTCTGAGGTC 
CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT 
CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC 
GCCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCGCC 
CGCGAGCTCT CGCTCGTCAC CCATGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT 
GTCTACTTGG AGGCTGTGAA GCCGCTGCTG TCACACGCTC CCCGCCCTCT GGAGCCGCCT 
CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC 
CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC 
ATCTGTGTTG CCCCCTACCC GAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG 
GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGCGC TCAGGGCTCT GCGCGCCACC 
TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG 
GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCCACTGTGG GGTGGTGGGC 
CTTTTACCCC CTGGTGCAGC AGCCCCCTCG GGCTGGGTCC AGGCCGCCCT CAGTGACACC 
AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG 
GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCA GCCCCCATCC 
GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA 
AAGCTGGACA AGGCAGCCTC TCACCTCCGG CAGCTGGTGG ATGCGTCTCC AAGCCCTGGG 
GGCTCACCAT CCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_006052313.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006052375.2 Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu54227
Clone ID Related Accession (Same CDS sequence)	XM_006052312.2 , XM_006052313.2
Accession Version	XM_006052313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	valine--tRNA ligase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006052313.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCGTCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGGGTGATAC GGAGCACTCC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC 
AGAGAGGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC 
CCTGATGTCC TGGACACATG GTTCTCCTCG GCTCTTTTCC CCTTTTCTGC CCTAGGCTGG 
CCCCGAGAGA CCCTGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT 
GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG 
CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG 
GTACTGCAGG ACAAGCTGAG GGATGGAAAC TTGGACCCCA CAGAGCTGGC GATTGCAGCC 
TCGGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG 
TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGCGACTTGC ACCTGTCCGT CTCTGAGGTC 
CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT 
CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC 
GCCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCGCC 
CGCGAGCTCT CGCTCGTCAC CCATGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT 
GTCTACTTGG AGGCTGTGAA GCCGCTGCTG TCACACGCTC CCCGCCCTCT GGAGCCGCCT 
CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC 
CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC 
ATCTGTGTTG CCCCCTACCC GAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG 
GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGCGC TCAGGGCTCT GCGCGCCACC 
TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG 
GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCCACTGTGG GGTGGTGGGC 
CTTTTACCCC CTGGTGCAGC AGCCCCCTCG GGCTGGGTCC AGGCCGCCCT CAGTGACACC 
AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG 
GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCA GCCCCCATCC 
GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA 
AAGCTGGACA AGGCAGCCTC TCACCTCCGG CAGCTGGTGG ATGCGTCTCC AAGCCCTGGG 
GGCTCACCAT CCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006052375.2
CDS	57..3254
Translation	MPHLPLTSFRPPLWGLRLSRGLPRSRPLSTQSEPDGSAVSRRNREAKQKRLREKQASLEAGIAPKSKSPAESSKAWTPKERVLYEISTEHGEKKDVSRPLPPAYSPQYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREDFLREVWKWKEEKGGEICEQLRALGASLDWDRECFTMDTGSSMAVTEAFVRLYKAGLLYRSRQLVNWSCALRSAISDIEVESRPLPGRTELQLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLCHPLTGQLLPLITDCTVQPRLGTGAVKVTPAHSPADAELGARHGLSPRSVIAEDGTMTALCEDWLQGLHRFVAREKILSALRERGLFRGLQNHPMVLPICSRSGDVVEYLLKSQWFVRCREMGERAAKAVESGALDLSPSFHQKNWQHWFSHIGDWCVSRQLWWGHRIPAYLVVEEHAKGDTEHSWVVGRTEAEAREVAAELTGRPGAELTLERDPDVLDTWFSSALFPFSALGWPRETLDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGVELQVLQDKLRDGNLDPTELAIAASAQRKDFPHGIPECGTDALRFTLCSHGALGGDLHLSVSEVLSFRHFCNKIWNALRFILNALGEEFIPQPAEELSPASRMDAWILSCLARTAQDCERGFLARELSLVTHALHHFWLHNLCDVYLEAVKPLLSHAPRPLEPPQVLFFCADVGLRLLAPLMPFLAEELWQRLPPRPGGPSAPSICVAPYPSARSLEHWHQPELERRFSRVQESVQALRALRATYQLTKARPRVLLQSSEPGEQGLFEAFLEPLGILGHCGVVGLLPPGAAAPSGWVQAALSDTSQAYMELQGLVDPQTHLPRLAARRHKLQKQLDGLLAQPPSEGQAETQRQQRLSSLQLELSKLDKAASHLRQLVDASPSPGGSPSP

Target ORF information:

RefSeq Version	XM_006052313.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006052313.2

ORF Insert Sequence:

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCGTCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGGGTGATAC GGAGCACTCC TGGGTGGTCG GGCGGACAGA GGCTGAGGCC 
AGAGAGGTAG CTGCAGAATT AACAGGGAGA CCAGGGGCGG AGCTGACCCT GGAGAGGGAC 
CCTGATGTCC TGGACACATG GTTCTCCTCG GCTCTTTTCC CCTTTTCTGC CCTAGGCTGG 
CCCCGAGAGA CCCTGGACCT GGCTCGTTTC TACCCCCTGT CACTTTTGGA AACGGGCAGT 
GACCTCCTGC TCTTCTGGGT GGGCCGCATG GTCATGTTGG GGACCCAGCT CACAGGGCAG 
CTCCCCTTCA GCAAGGTCCT GCTTCACTCC ATGGTCCGGG ACAGGCAGGG CCGGAAGATG 
AGCAAGTCCC TGGGGAACGT GCTGGACCCA CGGGACATCA TCAGTGGGGT GGAGCTGCAG 
GTACTGCAGG ACAAGCTGAG GGATGGAAAC TTGGACCCCA CAGAGCTGGC GATTGCAGCC 
TCGGCGCAGA GAAAGGACTT CCCTCATGGG ATCCCCGAGT GTGGGACAGA TGCTCTGAGG 
TTCACCCTGT GCTCCCATGG GGCCCTGGGA GGCGACTTGC ACCTGTCCGT CTCTGAGGTC 
CTGAGCTTCC GACACTTCTG CAATAAGATC TGGAATGCCC TGCGCTTTAT CCTGAATGCT 
CTTGGGGAGG AATTCATACC CCAACCTGCG GAGGAGCTGT CCCCCGCCTC CCGCATGGAC 
GCCTGGATCC TCAGCTGCCT GGCCCGCACG GCCCAGGACT GCGAGCGGGG CTTCCTCGCC 
CGCGAGCTCT CGCTCGTCAC CCATGCCCTG CACCACTTCT GGCTGCACAA CCTCTGTGAT 
GTCTACTTGG AGGCTGTGAA GCCGCTGCTG TCACACGCTC CCCGCCCTCT GGAGCCGCCT 
CAGGTCCTGT TCTTCTGCGC AGACGTCGGT CTCCGCCTCC TCGCCCCGTT GATGCCTTTC 
CTGGCTGAAG AGCTCTGGCA GAGGCTGCCC CCCAGGCCTG GTGGTCCCTC TGCCCCCAGC 
ATCTGTGTTG CCCCCTACCC GAGTGCCCGC AGCTTGGAGC ACTGGCACCA GCCTGAGCTG 
GAGAGGCGCT TCTCCCGGGT TCAGGAGTCC GTGCAGGCGC TCAGGGCTCT GCGCGCCACC 
TACCAGCTCA CCAAGGCCCG GCCCCGAGTG CTGCTGCAGA GCTCAGAGCC AGGCGAGCAG 
GGCCTCTTTG AGGCCTTCCT GGAGCCGCTG GGCATCCTGG GCCACTGTGG GGTGGTGGGC 
CTTTTACCCC CTGGTGCAGC AGCCCCCTCG GGCTGGGTCC AGGCCGCCCT CAGTGACACC 
AGTCAGGCGT ATATGGAGCT GCAGGGCCTG GTGGACCCCC AGACCCACCT ACCTCGGCTG 
GCTGCCCGAA GACACAAGTT GCAGAAGCAG CTTGATGGCC TCCTAGCCCA GCCCCCATCC 
GAGGGACAGG CGGAGACTCA GAGGCAGCAG AGGCTTTCTT CCCTCCAGTT GGAGTTGTCA 
AAGCTGGACA AGGCAGCCTC TCACCTCCGG CAGCTGGTGG ATGCGTCTCC AAGCCCTGGG 
GGCTCACCAT CCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VARS2 ( XM_025296492.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025152277.1 Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.

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CloneID	OBu54228
Clone ID Related Accession (Same CDS sequence)	XM_025296492.1
Accession Version	XM_025296492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3021bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	valine--tRNA ligase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037546.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCGTCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGACCCTGGA CCTGGCTCGT TTCTACCCCC TGTCACTTTT GGAAACGGGC 
AGTGACCTCC TGCTCTTCTG GGTGGGCCGC ATGGTCATGT TGGGGACCCA GCTCACAGGG 
CAGCTCCCCT TCAGCAAGGT CCTGCTTCAC TCCATGGTCC GGGACAGGCA GGGCCGGAAG 
ATGAGCAAGT CCCTGGGGAA CGTGCTGGAC CCACGGGACA TCATCAGTGG GGTGGAGCTG 
CAGGTACTGC AGGACAAGCT GAGGGATGGA AACTTGGACC CCACAGAGCT GGCGATTGCA 
GCCTCGGCGC AGAGAAAGGA CTTCCCTCAT GGGATCCCCG AGTGTGGGAC AGATGCTCTG 
AGGTTCACCC TGTGCTCCCA TGGGGCCCTG GGAGGCGACT TGCACCTGTC CGTCTCTGAG 
GTCCTGAGCT TCCGACACTT CTGCAATAAG ATCTGGAATG CCCTGCGCTT TATCCTGAAT 
GCTCTTGGGG AGGAATTCAT ACCCCAACCT GCGGAGGAGC TGTCCCCCGC CTCCCGCATG 
GACGCCTGGA TCCTCAGCTG CCTGGCCCGC ACGGCCCAGG ACTGCGAGCG GGGCTTCCTC 
GCCCGCGAGC TCTCGCTCGT CACCCATGCC CTGCACCACT TCTGGCTGCA CAACCTCTGT 
GATGTCTACT TGGAGGCTGT GAAGCCGCTG CTGTCACACG CTCCCCGCCC TCTGGAGCCG 
CCTCAGGTCC TGTTCTTCTG CGCAGACGTC GGTCTCCGCC TCCTCGCCCC GTTGATGCCT 
TTCCTGGCTG AAGAGCTCTG GCAGAGGCTG CCCCCCAGGC CTGGTGGTCC CTCTGCCCCC 
AGCATCTGTG TTGCCCCCTA CCCGAGTGCC CGCAGCTTGG AGCACTGGCA CCAGCCTGAG 
CTGGAGAGGC GCTTCTCCCG GGTTCAGGAG TCCGTGCAGG CGCTCAGGGC TCTGCGCGCC 
ACCTACCAGC TCACCAAGGC CCGGCCCCGA GTGCTGCTGC AGAGCTCAGA GCCAGGCGAG 
CAGGGCCTCT TTGAGGCCTT CCTGGAGCCG CTGGGCATCC TGGGCCACTG TGGGGTGGTG 
GGCCTTTTAC CCCCTGGTGC AGCAGCCCCC TCGGGCTGGG TCCAGGCCGC CCTCAGTGAC 
ACCAGTCAGG CGTATATGGA GCTGCAGGGC CTGGTGGACC CCCAGACCCA CCTACCTCGG 
CTGGCTGCCC GAAGACACAA GTTGCAGAAG CAGCTTGATG GCCTCCTAGC CCAGCCCCCA 
TCCGAGGGAC AGGCGGAGAC TCAGAGGCAG CAGAGGCTTT CTTCCCTCCA GTTGGAGTTG 
TCAAAGCTGG ACAAGGCAGC CTCTCACCTC CGGCAGCTGG TGGATGCGTC TCCAAGCCCT 
GGGGGCTCAC CATCCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025152277.1
CDS	170..3190
Translation	MPHLPLTSFRPPLWGLRLSRGLPRSRPLSTQSEPDGSAVSRRNREAKQKRLREKQASLEAGIAPKSKSPAESSKAWTPKERVLYEISTEHGEKKDVSRPLPPAYSPQYVEAAWYPWWVREGFFKPEYQARLPQATGETFSMCIPPPNVTGSLHIGHALTVAIQDALVRWHRMRGDRVLWVPGSDHAGIATQAVVEKQLWKERGVRRHELSREDFLREVWKWKEEKGGEICEQLRALGASLDWDRECFTMDTGSSMAVTEAFVRLYKAGLLYRSRQLVNWSCALRSAISDIEVESRPLPGRTELQLPGCPTPVSFGLLFSVAFPVDGEPDAEVVVGTTRPETLPGDVAVAVHPDDSRYTHLHGRQLCHPLTGQLLPLITDCTVQPRLGTGAVKVTPAHSPADAELGARHGLSPRSVIAEDGTMTALCEDWLQGLHRFVAREKILSALRERGLFRGLQNHPMVLPICSRSGDVVEYLLKSQWFVRCREMGERAAKAVESGALDLSPSFHQKNWQHWFSHIGDWCVSRQLWWGHRIPAYLVVEEHAKTLDLARFYPLSLLETGSDLLLFWVGRMVMLGTQLTGQLPFSKVLLHSMVRDRQGRKMSKSLGNVLDPRDIISGVELQVLQDKLRDGNLDPTELAIAASAQRKDFPHGIPECGTDALRFTLCSHGALGGDLHLSVSEVLSFRHFCNKIWNALRFILNALGEEFIPQPAEELSPASRMDAWILSCLARTAQDCERGFLARELSLVTHALHHFWLHNLCDVYLEAVKPLLSHAPRPLEPPQVLFFCADVGLRLLAPLMPFLAEELWQRLPPRPGGPSAPSICVAPYPSARSLEHWHQPELERRFSRVQESVQALRALRATYQLTKARPRVLLQSSEPGEQGLFEAFLEPLGILGHCGVVGLLPPGAAAPSGWVQAALSDTSQAYMELQGLVDPQTHLPRLAARRHKLQKQLDGLLAQPPSEGQAETQRQQRLSSLQLELSKLDKAASHLRQLVDASPSPGGSPSP

Target ORF information:

RefSeq Version	XM_025296492.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis valyl-tRNA synthetase 2, mitochondrial (VARS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025296492.1

ORF Insert Sequence:

ATGCCTCATT TGCCTCTGAC CTCTTTTCGA CCACCGCTTT GGGGCTTGAG GCTCTCACGG 
GGCCTCCCCA GGTCCCGTCC CCTTTCCACC CAGTCAGAGC CAGATGGATC AGCCGTCTCG 
CGGAGGAACC GTGAAGCCAA ACAGAAGCGC CTGCGAGAGA AGCAGGCATC GCTGGAGGCT 
GGGATAGCCC CCAAGAGCAA GTCACCTGCA GAATCCAGTA AGGCCTGGAC CCCTAAGGAG 
AGAGTGTTGT ATGAAATCTC CACGGAACAT GGAGAAAAGA AAGATGTCTC CCGACCCCTG 
CCTCCTGCAT ACAGCCCCCA GTACGTTGAG GCTGCCTGGT ACCCTTGGTG GGTGCGAGAG 
GGCTTCTTCA AACCAGAATA TCAGGCCAGG CTGCCCCAGG CCACAGGGGA GACCTTTTCC 
ATGTGCATCC CACCCCCTAA TGTCACAGGC TCCCTGCACA TTGGGCATGC GCTGACCGTG 
GCCATACAGG ATGCTCTCGT GCGCTGGCAC CGGATGCGTG GGGATCGGGT GCTGTGGGTC 
CCTGGCTCAG ATCATGCAGG GATTGCGACC CAAGCTGTGG TGGAGAAACA GCTGTGGAAG 
GAGCGAGGCG TGAGGAGACA TGAGCTGAGC CGGGAAGACT TCCTTAGGGA GGTGTGGAAG 
TGGAAGGAGG AGAAAGGTGG CGAGATCTGT GAGCAGCTCC GAGCGCTGGG GGCCTCCCTG 
GACTGGGACC GAGAGTGCTT TACCATGGAT ACCGGCTCCT CAATGGCCGT GACAGAAGCT 
TTCGTGCGGC TCTACAAGGC TGGGTTGTTG TACCGGAGCC GGCAGCTGGT CAACTGGTCG 
TGTGCCTTAC GCTCCGCCAT CTCGGATATT GAGGTGGAGA GCCGGCCCCT CCCTGGCCGC 
ACGGAGCTTC AGTTGCCTGG CTGCCCCACC CCCGTGTCTT TCGGCCTCCT CTTTTCCGTG 
GCCTTCCCCG TGGATGGAGA GCCTGACGCA GAGGTTGTGG TGGGAACCAC GAGGCCAGAG 
ACGCTGCCTG GAGACGTGGC TGTGGCGGTC CATCCTGATG ACTCCCGCTA CACGCATCTA 
CATGGGCGAC AACTCTGTCA TCCCTTGACG GGGCAGCTTC TCCCCCTTAT CACAGACTGC 
ACTGTTCAGC CACGCCTGGG CACAGGGGCA GTGAAGGTGA CTCCAGCGCA CAGCCCTGCC 
GATGCTGAGC TGGGGGCCCG ACACGGCCTG AGCCCCCGGA GTGTCATTGC GGAGGATGGG 
ACCATGACCG CCCTTTGTGA GGACTGGCTG CAGGGTCTTC ACCGATTTGT GGCCCGGGAA 
AAGATTCTGT CAGCGCTGAG GGAGCGGGGC CTGTTCCGGG GCCTCCAAAA CCACCCCATG 
GTGCTGCCCA TTTGCAGCCG TTCCGGGGAT GTGGTAGAGT ACCTACTGAA GAGCCAGTGG 
TTTGTCCGCT GCCGGGAAAT GGGGGAGCGA GCCGCCAAGG CCGTGGAGTC GGGGGCCCTG 
GACCTTAGTC CCTCCTTCCA CCAGAAGAAC TGGCAGCACT GGTTTTCCCA CATTGGGGAC 
TGGTGTGTCT CCCGGCAGCT GTGGTGGGGC CATCGGATTC CAGCCTACCT GGTTGTAGAA 
GAACATGCAA AGACCCTGGA CCTGGCTCGT TTCTACCCCC TGTCACTTTT GGAAACGGGC 
AGTGACCTCC TGCTCTTCTG GGTGGGCCGC ATGGTCATGT TGGGGACCCA GCTCACAGGG 
CAGCTCCCCT TCAGCAAGGT CCTGCTTCAC TCCATGGTCC GGGACAGGCA GGGCCGGAAG 
ATGAGCAAGT CCCTGGGGAA CGTGCTGGAC CCACGGGACA TCATCAGTGG GGTGGAGCTG 
CAGGTACTGC AGGACAAGCT GAGGGATGGA AACTTGGACC CCACAGAGCT GGCGATTGCA 
GCCTCGGCGC AGAGAAAGGA CTTCCCTCAT GGGATCCCCG AGTGTGGGAC AGATGCTCTG 
AGGTTCACCC TGTGCTCCCA TGGGGCCCTG GGAGGCGACT TGCACCTGTC CGTCTCTGAG 
GTCCTGAGCT TCCGACACTT CTGCAATAAG ATCTGGAATG CCCTGCGCTT TATCCTGAAT 
GCTCTTGGGG AGGAATTCAT ACCCCAACCT GCGGAGGAGC TGTCCCCCGC CTCCCGCATG 
GACGCCTGGA TCCTCAGCTG CCTGGCCCGC ACGGCCCAGG ACTGCGAGCG GGGCTTCCTC 
GCCCGCGAGC TCTCGCTCGT CACCCATGCC CTGCACCACT TCTGGCTGCA CAACCTCTGT 
GATGTCTACT TGGAGGCTGT GAAGCCGCTG CTGTCACACG CTCCCCGCCC TCTGGAGCCG 
CCTCAGGTCC TGTTCTTCTG CGCAGACGTC GGTCTCCGCC TCCTCGCCCC GTTGATGCCT 
TTCCTGGCTG AAGAGCTCTG GCAGAGGCTG CCCCCCAGGC CTGGTGGTCC CTCTGCCCCC 
AGCATCTGTG TTGCCCCCTA CCCGAGTGCC CGCAGCTTGG AGCACTGGCA CCAGCCTGAG 
CTGGAGAGGC GCTTCTCCCG GGTTCAGGAG TCCGTGCAGG CGCTCAGGGC TCTGCGCGCC 
ACCTACCAGC TCACCAAGGC CCGGCCCCGA GTGCTGCTGC AGAGCTCAGA GCCAGGCGAG 
CAGGGCCTCT TTGAGGCCTT CCTGGAGCCG CTGGGCATCC TGGGCCACTG TGGGGTGGTG 
GGCCTTTTAC CCCCTGGTGC AGCAGCCCCC TCGGGCTGGG TCCAGGCCGC CCTCAGTGAC 
ACCAGTCAGG CGTATATGGA GCTGCAGGGC CTGGTGGACC CCCAGACCCA CCTACCTCGG 
CTGGCTGCCC GAAGACACAA GTTGCAGAAG CAGCTTGATG GCCTCCTAGC CCAGCCCCCA 
TCCGAGGGAC AGGCGGAGAC TCAGAGGCAG CAGAGGCTTT CTTCCCTCCA GTTGGAGTTG 
TCAAAGCTGG ACAAGGCAGC CTCTCACCTC CGGCAGCTGG TGGATGCGTC TCCAAGCCCT 
GGGGGCTCAC CATCCCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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VARS2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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