BDNF cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BDNF
Entrez Gene ID	617701
Full Name	brain derived neurotrophic factor
General protein information	Preferred Names brain derived neurotrophic factor Names brain-derived neurotrophic factor
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005216335.3	XP_005216392.1	brain-derived neurotrophic factor isoform X2
XM_005216335.4	XP_005216392.1	brain-derived neurotrophic factor isoform X2
XM_005216334.3	XP_005216391.1	brain-derived neurotrophic factor isoform X1
XM_005216334.4	XP_005216391.1	brain-derived neurotrophic factor isoform X1
NM_001046607.2	NP_001040072.1	brain-derived neurotrophic factor precursor
XM_005216336.3	XP_005216393.1	brain-derived neurotrophic factor isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BDNF	NP_001026787.1
Canis lupus familiaris (dog)	BDNF	NP_001002975.1
Bos taurus (cattle)	BDNF	NP_001040072.1
Homo sapiens (human)	BDNF	NP_001137282.1
Pan troglodytes (chimpanzee)	BDNF	NP_001012443.1
Mus musculus (house mouse)	Bdnf	NP_031566.4
Danio rerio (zebrafish)	bdnf	NP_571670.2
Rattus norvegicus (Norway rat)	Bdnf	NP_036645.2
Xenopus tropicalis (tropical clawed frog)	bdnf	NP_001090767.1
	BDNF	XP_001089568.2

Gene Ontology
Bibliography

Related articles in PubMed

The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm.
Li C, Li C, Zhu X, Wang C, Liu Z, Li W, Lu C, Zhou X
Theriogenology77(3)636-43(2012 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Expression of brain-derived neurotrophic factor and tropomyosin-related kinase-B in a bovine jejunal nodular ganglioneuroblastoma.
Ulrich R, Stan AC, Koch A, Beineke A
Veterinary pathology45(3)355-60(2008 May)

The gene encoding bovine brain-derived neurotrophic factor (BDNF).
Arab SF, Krohn K, Lachmund A, Unsicker K, Suter-Crazzolara C
Gene185(1)95-8(1997 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These observations provided evidence that brain-derived naeurotrophic factor(BDNF) and its receptor (BDNF receptor) secreted by bovine sperm was important in regulation of insulin and leptin.
Title: The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm.

Expressed in ganglionic neuron-like tumor cells, which may activate an embryonic pathway involving BDNF.
Title: Expression of brain-derived neurotrophic factor and tropomyosin-related kinase-B in a bovine jejunal nodular ganglioneuroblastoma.

The following BDNF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BDNF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa01200	NM_001046607.2 Latest version!	Bos taurus brain derived neurotrophic factor (BDNF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa01200	XM_005216336.3 Latest version!	Bos taurus brain-derived neurotrophic factor (BDNF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa01200	XM_005216335.4 Latest version!	Bos taurus brain derived neurotrophic factor (BDNF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa26652	XM_005216334.4 Latest version!	Bos taurus brain derived neurotrophic factor (BDNF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
OBa01200	XM_005216335.3	Bos taurus brain-derived neurotrophic factor (BDNF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OBa26652	XM_005216334.3	Bos taurus brain-derived neurotrophic factor (BDNF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BDNF ( NM_001046607.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001040072.1 Bos taurus brain derived neurotrophic factor (BDNF), mRNA.

Price & Availability↑

CloneID	OBa01200
Clone ID Related Accession (Same CDS sequence)	XM_005216335.3 , XM_005216335.4 , NM_001046607.2 , XM_005216336.3
Accession Version	NM_001046607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	brain-derived neurotrophic factor precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC109860.1. On Aug 31, 2012 this sequence version replaced NM_001046607.1. ##Evidence-Data-START## Transcript exon combination :: BC109860.1, FD698038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145429 [ECO:0000348] ##Evidence-Data-END##

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CCAACCTCCG AGCCCAAGGC AGCTTGGCCT ACCCAGGTGT GCGGACCCAT 
GGGACTCTGG AGAGCATGAA TGGGCCCAAG GTGGGTTCAA GAGGCCTGAC GTCCTCGTCG 
TCGTTGGCTG ACACTTTTGA ACACGTGATC GAAGAGCTGT TGGACGAGGA CCAGAAAGTT 
CGGCCCAGCG AGGAAAACAA TAAAGACGCG GACATGTACA CGTCCCGGGT GATGCTCAGC 
AGTCAAGTGC CTTTGGAGCC CCCTCTCCTC TTCCTGCTCG AGGAATACAA AAATTACCTG 
GATGCCGCAA ACATGTCCAT GAGGGTCCGG CGCCACTCGG ACCCCGCCCG CCGCGGGGAG 
CTGAGCGTGT GTGACAGCAT CAGCGAGTGG GTGACCGCAG CGGATAAAAA GACTGCAGTG 
GACATGTCGG GCGGGACGGT CACGGTCCTT GAAAAAGTCC CCGTCTCAAA AGGCCAGCTG 
AAGCAGTACT TCTACGAGAC CAAGTGCAAT CCCATGGGTT ACACGAAGGA GGGCTGCAGG 
GGCATAGACA AGAGGCATTG GAACTCCCAG TGCCGAACTA CCCAGTCGTA CGTGCGGGCC 
CTCACCATGG ATAGCAAAAA GCGTATTGGC TGGCGGTTCA TACGGATAGA CACTTCTTGT 
GTATGTACAC TGACCATTAA GAGGGGAAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001040072.1
CDS	169..921
Translation	MTILFLTMVISYFGCMKAAPMKEANLRAQGSLAYPGVRTHGTLESMNGPKVGSRGLTSSSSLADTFEHVIEELLDEDQKVRPSEENNKDADMYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	NM_001046607.2
Organism	Bos taurus(cattle)
Definition	Bos taurus brain derived neurotrophic factor (BDNF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001046607.2

ORF Insert Sequence:

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CCAACCTCCG AGCCCAAGGC AGCTTGGCCT ACCCAGGTGT GCGGACCCAT 
GGGACTCTGG AGAGCATGAA TGGGCCCAAG GTGGGTTCAA GAGGCCTGAC GTCCTCGTCG 
TCGTTGGCTG ACACTTTTGA ACACGTGATC GAAGAGCTGT TGGACGAGGA CCAGAAAGTT 
CGGCCCAGCG AGGAAAACAA TAAAGACGCG GACATGTACA CGTCCCGGGT GATGCTCAGC 
AGTCAAGTGC CTTTGGAGCC CCCTCTCCTC TTCCTGCTCG AGGAATACAA AAATTACCTG 
GATGCCGCAA ACATGTCCAT GAGGGTCCGG CGCCACTCGG ACCCCGCCCG CCGCGGGGAG 
CTGAGCGTGT GTGACAGCAT CAGCGAGTGG GTGACCGCAG CGGATAAAAA GACTGCAGTG 
GACATGTCGG GCGGGACGGT CACGGTCCTT GAAAAAGTCC CCGTCTCAAA AGGCCAGCTG 
AAGCAGTACT TCTACGAGAC CAAGTGCAAT CCCATGGGTT ACACGAAGGA GGGCTGCAGG 
GGCATAGACA AGAGGCATTG GAACTCCCAG TGCCGAACTA CCCAGTCGTA CGTGCGGGCC 
CTCACCATGG ATAGCAAAAA GCGTATTGGC TGGCGGTTCA TACGGATAGA CACTTCTTGT 
GTATGTACAC TGACCATTAA GAGGGGAAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDNF ( XM_005216335.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216392.1 Bos taurus brain-derived neurotrophic factor (BDNF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa01200
Clone ID Related Accession (Same CDS sequence)	XM_005216335.3 , XM_005216335.4 , NM_001046607.2 , XM_005216336.3
Accession Version	XM_005216335.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	brain-derived neurotrophic factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104418.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216335.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CCAACCTCCG AGCCCAAGGC AGCTTGGCCT ACCCAGGTGT GCGGACCCAT 
GGGACTCTGG AGAGCATGAA TGGGCCCAAG GTGGGTTCAA GAGGCCTGAC GTCCTCGTCG 
TCGTTGGCTG ACACTTTTGA ACACGTGATC GAAGAGCTGT TGGACGAGGA CCAGAAAGTT 
CGGCCCAGCG AGGAAAACAA TAAAGACGCG GACATGTACA CGTCCCGGGT GATGCTCAGC 
AGTCAAGTGC CTTTGGAGCC CCCTCTCCTC TTCCTGCTCG AGGAATACAA AAATTACCTG 
GATGCCGCAA ACATGTCCAT GAGGGTCCGG CGCCACTCGG ACCCCGCCCG CCGCGGGGAG 
CTGAGCGTGT GTGACAGCAT CAGCGAGTGG GTGACCGCAG CGGATAAAAA GACTGCAGTG 
GACATGTCGG GCGGGACGGT CACGGTCCTT GAAAAAGTCC CCGTCTCAAA AGGCCAGCTG 
AAGCAGTACT TCTACGAGAC CAAGTGCAAT CCCATGGGTT ACACGAAGGA GGGCTGCAGG 
GGCATAGACA AGAGGCATTG GAACTCCCAG TGCCGAACTA CCCAGTCGTA CGTGCGGGCC 
CTCACCATGG ATAGCAAAAA GCGTATTGGC TGGCGGTTCA TACGGATAGA CACTTCTTGT 
GTATGTACAC TGACCATTAA GAGGGGAAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216392.1
CDS	302..1054
Translation	MTILFLTMVISYFGCMKAAPMKEANLRAQGSLAYPGVRTHGTLESMNGPKVGSRGLTSSSSLADTFEHVIEELLDEDQKVRPSEENNKDADMYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_005216335.3
Organism	Bos taurus(cattle)
Definition	Bos taurus brain-derived neurotrophic factor (BDNF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216335.3

ORF Insert Sequence:

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CCAACCTCCG AGCCCAAGGC AGCTTGGCCT ACCCAGGTGT GCGGACCCAT 
GGGACTCTGG AGAGCATGAA TGGGCCCAAG GTGGGTTCAA GAGGCCTGAC GTCCTCGTCG 
TCGTTGGCTG ACACTTTTGA ACACGTGATC GAAGAGCTGT TGGACGAGGA CCAGAAAGTT 
CGGCCCAGCG AGGAAAACAA TAAAGACGCG GACATGTACA CGTCCCGGGT GATGCTCAGC 
AGTCAAGTGC CTTTGGAGCC CCCTCTCCTC TTCCTGCTCG AGGAATACAA AAATTACCTG 
GATGCCGCAA ACATGTCCAT GAGGGTCCGG CGCCACTCGG ACCCCGCCCG CCGCGGGGAG 
CTGAGCGTGT GTGACAGCAT CAGCGAGTGG GTGACCGCAG CGGATAAAAA GACTGCAGTG 
GACATGTCGG GCGGGACGGT CACGGTCCTT GAAAAAGTCC CCGTCTCAAA AGGCCAGCTG 
AAGCAGTACT TCTACGAGAC CAAGTGCAAT CCCATGGGTT ACACGAAGGA GGGCTGCAGG 
GGCATAGACA AGAGGCATTG GAACTCCCAG TGCCGAACTA CCCAGTCGTA CGTGCGGGCC 
CTCACCATGG ATAGCAAAAA GCGTATTGGC TGGCGGTTCA TACGGATAGA CACTTCTTGT 
GTATGTACAC TGACCATTAA GAGGGGAAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDNF ( XM_005216335.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216392.1 Bos taurus brain derived neurotrophic factor (BDNF), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa01200
Clone ID Related Accession (Same CDS sequence)	XM_005216335.3 , XM_005216335.4 , NM_001046607.2 , XM_005216336.3
Accession Version	XM_005216335.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	brain-derived neurotrophic factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216335.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CCAACCTCCG AGCCCAAGGC AGCTTGGCCT ACCCAGGTGT GCGGACCCAT 
GGGACTCTGG AGAGCATGAA TGGGCCCAAG GTGGGTTCAA GAGGCCTGAC GTCCTCGTCG 
TCGTTGGCTG ACACTTTTGA ACACGTGATC GAAGAGCTGT TGGACGAGGA CCAGAAAGTT 
CGGCCCAGCG AGGAAAACAA TAAAGACGCG GACATGTACA CGTCCCGGGT GATGCTCAGC 
AGTCAAGTGC CTTTGGAGCC CCCTCTCCTC TTCCTGCTCG AGGAATACAA AAATTACCTG 
GATGCCGCAA ACATGTCCAT GAGGGTCCGG CGCCACTCGG ACCCCGCCCG CCGCGGGGAG 
CTGAGCGTGT GTGACAGCAT CAGCGAGTGG GTGACCGCAG CGGATAAAAA GACTGCAGTG 
GACATGTCGG GCGGGACGGT CACGGTCCTT GAAAAAGTCC CCGTCTCAAA AGGCCAGCTG 
AAGCAGTACT TCTACGAGAC CAAGTGCAAT CCCATGGGTT ACACGAAGGA GGGCTGCAGG 
GGCATAGACA AGAGGCATTG GAACTCCCAG TGCCGAACTA CCCAGTCGTA CGTGCGGGCC 
CTCACCATGG ATAGCAAAAA GCGTATTGGC TGGCGGTTCA TACGGATAGA CACTTCTTGT 
GTATGTACAC TGACCATTAA GAGGGGAAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216392.1
CDS	305..1057
Translation	MTILFLTMVISYFGCMKAAPMKEANLRAQGSLAYPGVRTHGTLESMNGPKVGSRGLTSSSSLADTFEHVIEELLDEDQKVRPSEENNKDADMYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_005216335.4
Organism	Bos taurus(cattle)
Definition	Bos taurus brain derived neurotrophic factor (BDNF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216335.4

ORF Insert Sequence:

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CCAACCTCCG AGCCCAAGGC AGCTTGGCCT ACCCAGGTGT GCGGACCCAT 
GGGACTCTGG AGAGCATGAA TGGGCCCAAG GTGGGTTCAA GAGGCCTGAC GTCCTCGTCG 
TCGTTGGCTG ACACTTTTGA ACACGTGATC GAAGAGCTGT TGGACGAGGA CCAGAAAGTT 
CGGCCCAGCG AGGAAAACAA TAAAGACGCG GACATGTACA CGTCCCGGGT GATGCTCAGC 
AGTCAAGTGC CTTTGGAGCC CCCTCTCCTC TTCCTGCTCG AGGAATACAA AAATTACCTG 
GATGCCGCAA ACATGTCCAT GAGGGTCCGG CGCCACTCGG ACCCCGCCCG CCGCGGGGAG 
CTGAGCGTGT GTGACAGCAT CAGCGAGTGG GTGACCGCAG CGGATAAAAA GACTGCAGTG 
GACATGTCGG GCGGGACGGT CACGGTCCTT GAAAAAGTCC CCGTCTCAAA AGGCCAGCTG 
AAGCAGTACT TCTACGAGAC CAAGTGCAAT CCCATGGGTT ACACGAAGGA GGGCTGCAGG 
GGCATAGACA AGAGGCATTG GAACTCCCAG TGCCGAACTA CCCAGTCGTA CGTGCGGGCC 
CTCACCATGG ATAGCAAAAA GCGTATTGGC TGGCGGTTCA TACGGATAGA CACTTCTTGT 
GTATGTACAC TGACCATTAA GAGGGGAAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDNF ( XM_005216336.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216393.1 Bos taurus brain-derived neurotrophic factor (BDNF), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa01200
Clone ID Related Accession (Same CDS sequence)	XM_005216335.3 , XM_005216335.4 , NM_001046607.2 , XM_005216336.3
Accession Version	XM_005216336.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	brain-derived neurotrophic factor isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104418.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216336.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CCAACCTCCG AGCCCAAGGC AGCTTGGCCT ACCCAGGTGT GCGGACCCAT 
GGGACTCTGG AGAGCATGAA TGGGCCCAAG GTGGGTTCAA GAGGCCTGAC GTCCTCGTCG 
TCGTTGGCTG ACACTTTTGA ACACGTGATC GAAGAGCTGT TGGACGAGGA CCAGAAAGTT 
CGGCCCAGCG AGGAAAACAA TAAAGACGCG GACATGTACA CGTCCCGGGT GATGCTCAGC 
AGTCAAGTGC CTTTGGAGCC CCCTCTCCTC TTCCTGCTCG AGGAATACAA AAATTACCTG 
GATGCCGCAA ACATGTCCAT GAGGGTCCGG CGCCACTCGG ACCCCGCCCG CCGCGGGGAG 
CTGAGCGTGT GTGACAGCAT CAGCGAGTGG GTGACCGCAG CGGATAAAAA GACTGCAGTG 
GACATGTCGG GCGGGACGGT CACGGTCCTT GAAAAAGTCC CCGTCTCAAA AGGCCAGCTG 
AAGCAGTACT TCTACGAGAC CAAGTGCAAT CCCATGGGTT ACACGAAGGA GGGCTGCAGG 
GGCATAGACA AGAGGCATTG GAACTCCCAG TGCCGAACTA CCCAGTCGTA CGTGCGGGCC 
CTCACCATGG ATAGCAAAAA GCGTATTGGC TGGCGGTTCA TACGGATAGA CACTTCTTGT 
GTATGTACAC TGACCATTAA GAGGGGAAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216393.1
CDS	357..1109
Translation	MTILFLTMVISYFGCMKAAPMKEANLRAQGSLAYPGVRTHGTLESMNGPKVGSRGLTSSSSLADTFEHVIEELLDEDQKVRPSEENNKDADMYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_005216336.3
Organism	Bos taurus(cattle)
Definition	Bos taurus brain-derived neurotrophic factor (BDNF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216336.3

ORF Insert Sequence:

ATGACCATCC TTTTCCTTAC TATGGTTATT TCATACTTCG GTTGCATGAA GGCTGCCCCC 
ATGAAAGAAG CCAACCTCCG AGCCCAAGGC AGCTTGGCCT ACCCAGGTGT GCGGACCCAT 
GGGACTCTGG AGAGCATGAA TGGGCCCAAG GTGGGTTCAA GAGGCCTGAC GTCCTCGTCG 
TCGTTGGCTG ACACTTTTGA ACACGTGATC GAAGAGCTGT TGGACGAGGA CCAGAAAGTT 
CGGCCCAGCG AGGAAAACAA TAAAGACGCG GACATGTACA CGTCCCGGGT GATGCTCAGC 
AGTCAAGTGC CTTTGGAGCC CCCTCTCCTC TTCCTGCTCG AGGAATACAA AAATTACCTG 
GATGCCGCAA ACATGTCCAT GAGGGTCCGG CGCCACTCGG ACCCCGCCCG CCGCGGGGAG 
CTGAGCGTGT GTGACAGCAT CAGCGAGTGG GTGACCGCAG CGGATAAAAA GACTGCAGTG 
GACATGTCGG GCGGGACGGT CACGGTCCTT GAAAAAGTCC CCGTCTCAAA AGGCCAGCTG 
AAGCAGTACT TCTACGAGAC CAAGTGCAAT CCCATGGGTT ACACGAAGGA GGGCTGCAGG 
GGCATAGACA AGAGGCATTG GAACTCCCAG TGCCGAACTA CCCAGTCGTA CGTGCGGGCC 
CTCACCATGG ATAGCAAAAA GCGTATTGGC TGGCGGTTCA TACGGATAGA CACTTCTTGT 
GTATGTACAC TGACCATTAA GAGGGGAAGA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDNF ( XM_005216334.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216391.1 Bos taurus brain-derived neurotrophic factor (BDNF), transcript variant X1, mRNA.

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CloneID	OBa26652
Clone ID Related Accession (Same CDS sequence)	XM_005216334.3 , XM_005216334.4
Accession Version	XM_005216334.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	brain-derived neurotrophic factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104418.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216334.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCACC AGGTGAGAAG AGTGATGACC ATCCTTTTCC TTACTATGGT TATTTCATAC 
TTCGGTTGCA TGAAGGCTGC CCCCATGAAA GAAGCCAACC TCCGAGCCCA AGGCAGCTTG 
GCCTACCCAG GTGTGCGGAC CCATGGGACT CTGGAGAGCA TGAATGGGCC CAAGGTGGGT 
TCAAGAGGCC TGACGTCCTC GTCGTCGTTG GCTGACACTT TTGAACACGT GATCGAAGAG 
CTGTTGGACG AGGACCAGAA AGTTCGGCCC AGCGAGGAAA ACAATAAAGA CGCGGACATG 
TACACGTCCC GGGTGATGCT CAGCAGTCAA GTGCCTTTGG AGCCCCCTCT CCTCTTCCTG 
CTCGAGGAAT ACAAAAATTA CCTGGATGCC GCAAACATGT CCATGAGGGT CCGGCGCCAC 
TCGGACCCCG CCCGCCGCGG GGAGCTGAGC GTGTGTGACA GCATCAGCGA GTGGGTGACC 
GCAGCGGATA AAAAGACTGC AGTGGACATG TCGGGCGGGA CGGTCACGGT CCTTGAAAAA 
GTCCCCGTCT CAAAAGGCCA GCTGAAGCAG TACTTCTACG AGACCAAGTG CAATCCCATG 
GGTTACACGA AGGAGGGCTG CAGGGGCATA GACAAGAGGC ATTGGAACTC CCAGTGCCGA 
ACTACCCAGT CGTACGTGCG GGCCCTCACC ATGGATAGCA AAAAGCGTAT TGGCTGGCGG 
TTCATACGGA TAGACACTTC TTGTGTATGT ACACTGACCA TTAAGAGGGG AAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216391.1
CDS	589..1365
Translation	MFHQVRRVMTILFLTMVISYFGCMKAAPMKEANLRAQGSLAYPGVRTHGTLESMNGPKVGSRGLTSSSSLADTFEHVIEELLDEDQKVRPSEENNKDADMYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_005216334.3
Organism	Bos taurus(cattle)
Definition	Bos taurus brain-derived neurotrophic factor (BDNF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216334.3

ORF Insert Sequence:

ATGTTCCACC AGGTGAGAAG AGTGATGACC ATCCTTTTCC TTACTATGGT TATTTCATAC 
TTCGGTTGCA TGAAGGCTGC CCCCATGAAA GAAGCCAACC TCCGAGCCCA AGGCAGCTTG 
GCCTACCCAG GTGTGCGGAC CCATGGGACT CTGGAGAGCA TGAATGGGCC CAAGGTGGGT 
TCAAGAGGCC TGACGTCCTC GTCGTCGTTG GCTGACACTT TTGAACACGT GATCGAAGAG 
CTGTTGGACG AGGACCAGAA AGTTCGGCCC AGCGAGGAAA ACAATAAAGA CGCGGACATG 
TACACGTCCC GGGTGATGCT CAGCAGTCAA GTGCCTTTGG AGCCCCCTCT CCTCTTCCTG 
CTCGAGGAAT ACAAAAATTA CCTGGATGCC GCAAACATGT CCATGAGGGT CCGGCGCCAC 
TCGGACCCCG CCCGCCGCGG GGAGCTGAGC GTGTGTGACA GCATCAGCGA GTGGGTGACC 
GCAGCGGATA AAAAGACTGC AGTGGACATG TCGGGCGGGA CGGTCACGGT CCTTGAAAAA 
GTCCCCGTCT CAAAAGGCCA GCTGAAGCAG TACTTCTACG AGACCAAGTG CAATCCCATG 
GGTTACACGA AGGAGGGCTG CAGGGGCATA GACAAGAGGC ATTGGAACTC CCAGTGCCGA 
ACTACCCAGT CGTACGTGCG GGCCCTCACC ATGGATAGCA AAAAGCGTAT TGGCTGGCGG 
TTCATACGGA TAGACACTTC TTGTGTATGT ACACTGACCA TTAAGAGGGG AAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BDNF ( XM_005216334.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216391.1 Bos taurus brain derived neurotrophic factor (BDNF), transcript variant X1, mRNA.

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CloneID	OBa26652
Clone ID Related Accession (Same CDS sequence)	XM_005216334.3 , XM_005216334.4
Accession Version	XM_005216334.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	brain-derived neurotrophic factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216334.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCACC AGGTGAGAAG AGTGATGACC ATCCTTTTCC TTACTATGGT TATTTCATAC 
TTCGGTTGCA TGAAGGCTGC CCCCATGAAA GAAGCCAACC TCCGAGCCCA AGGCAGCTTG 
GCCTACCCAG GTGTGCGGAC CCATGGGACT CTGGAGAGCA TGAATGGGCC CAAGGTGGGT 
TCAAGAGGCC TGACGTCCTC GTCGTCGTTG GCTGACACTT TTGAACACGT GATCGAAGAG 
CTGTTGGACG AGGACCAGAA AGTTCGGCCC AGCGAGGAAA ACAATAAAGA CGCGGACATG 
TACACGTCCC GGGTGATGCT CAGCAGTCAA GTGCCTTTGG AGCCCCCTCT CCTCTTCCTG 
CTCGAGGAAT ACAAAAATTA CCTGGATGCC GCAAACATGT CCATGAGGGT CCGGCGCCAC 
TCGGACCCCG CCCGCCGCGG GGAGCTGAGC GTGTGTGACA GCATCAGCGA GTGGGTGACC 
GCAGCGGATA AAAAGACTGC AGTGGACATG TCGGGCGGGA CGGTCACGGT CCTTGAAAAA 
GTCCCCGTCT CAAAAGGCCA GCTGAAGCAG TACTTCTACG AGACCAAGTG CAATCCCATG 
GGTTACACGA AGGAGGGCTG CAGGGGCATA GACAAGAGGC ATTGGAACTC CCAGTGCCGA 
ACTACCCAGT CGTACGTGCG GGCCCTCACC ATGGATAGCA AAAAGCGTAT TGGCTGGCGG 
TTCATACGGA TAGACACTTC TTGTGTATGT ACACTGACCA TTAAGAGGGG AAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216391.1
CDS	585..1361
Translation	MFHQVRRVMTILFLTMVISYFGCMKAAPMKEANLRAQGSLAYPGVRTHGTLESMNGPKVGSRGLTSSSSLADTFEHVIEELLDEDQKVRPSEENNKDADMYTSRVMLSSQVPLEPPLLFLLEEYKNYLDAANMSMRVRRHSDPARRGELSVCDSISEWVTAADKKTAVDMSGGTVTVLEKVPVSKGQLKQYFYETKCNPMGYTKEGCRGIDKRHWNSQCRTTQSYVRALTMDSKKRIGWRFIRIDTSCVCTLTIKRGR

Target ORF information:

RefSeq Version	XM_005216334.4
Organism	Bos taurus(cattle)
Definition	Bos taurus brain derived neurotrophic factor (BDNF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216334.4

ORF Insert Sequence:

ATGTTCCACC AGGTGAGAAG AGTGATGACC ATCCTTTTCC TTACTATGGT TATTTCATAC 
TTCGGTTGCA TGAAGGCTGC CCCCATGAAA GAAGCCAACC TCCGAGCCCA AGGCAGCTTG 
GCCTACCCAG GTGTGCGGAC CCATGGGACT CTGGAGAGCA TGAATGGGCC CAAGGTGGGT 
TCAAGAGGCC TGACGTCCTC GTCGTCGTTG GCTGACACTT TTGAACACGT GATCGAAGAG 
CTGTTGGACG AGGACCAGAA AGTTCGGCCC AGCGAGGAAA ACAATAAAGA CGCGGACATG 
TACACGTCCC GGGTGATGCT CAGCAGTCAA GTGCCTTTGG AGCCCCCTCT CCTCTTCCTG 
CTCGAGGAAT ACAAAAATTA CCTGGATGCC GCAAACATGT CCATGAGGGT CCGGCGCCAC 
TCGGACCCCG CCCGCCGCGG GGAGCTGAGC GTGTGTGACA GCATCAGCGA GTGGGTGACC 
GCAGCGGATA AAAAGACTGC AGTGGACATG TCGGGCGGGA CGGTCACGGT CCTTGAAAAA 
GTCCCCGTCT CAAAAGGCCA GCTGAAGCAG TACTTCTACG AGACCAAGTG CAATCCCATG 
GGTTACACGA AGGAGGGCTG CAGGGGCATA GACAAGAGGC ATTGGAACTC CCAGTGCCGA 
ACTACCCAGT CGTACGTGCG GGCCCTCACC ATGGATAGCA AAAAGCGTAT TGGCTGGCGG 
TTCATACGGA TAGACACTTC TTGTGTATGT ACACTGACCA TTAAGAGGGG AAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm.
Theriogenology77(3)636-43(2012 Feb)
Li C,Li C,Zhu X,Wang C,Liu Z,Li W,Lu C,Zhou X

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Expression of brain-derived neurotrophic factor and tropomyosin-related kinase-B in a bovine jejunal nodular ganglioneuroblastoma.
Veterinary pathology45(3)355-60(2008 May)
Ulrich R,Stan AC,Koch A,Beineke A

The gene encoding bovine brain-derived neurotrophic factor (BDNF).
Gene185(1)95-8(1997 Jan)
Arab SF,Krohn K,Lachmund A,Unsicker K,Suter-Crazzolara C

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BDNF cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム