ZBTB4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZBTB4
Entrez Gene ID	613607
Full Name	zinc finger and BTB domain containing 4
General protein information	Preferred Names zinc finger and BTB domain containing 4 Names zinc finger and BTB domain-containing protein 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005220243.4	XP_005220300.2	zinc finger and BTB domain-containing protein 4 isoform X1
XM_024980389.1	XP_024836157.1	zinc finger and BTB domain-containing protein 4 isoform X2
XM_005220241.3	XP_005220298.1	zinc finger and BTB domain-containing protein 4 isoform X1
NM_001206898.1	NP_001193827.1	zinc finger and BTB domain-containing protein 4
NM_001206898.2	NP_001193827.2	zinc finger and BTB domain-containing protein 4
XM_005220243.3	XP_005220300.1	zinc finger and BTB domain-containing protein 4 isoform X1
XM_005220241.4	XP_005220298.2	zinc finger and BTB domain-containing protein 4 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ZBTB4	XP_511299.3
Rattus norvegicus (Norway rat)	Zbtb4	XP_006246904.1
Homo sapiens (human)	ZBTB4	NP_065950.2
	LOC721849	XP_002808162.1
Mus musculus (house mouse)	Zbtb4	NP_083624.2
Canis lupus familiaris (dog)	ZBTB4	NP_001192091.1
Bos taurus (cattle)	ZBTB4	NP_001193827.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
Jann OC, Aerts J, Jones M, Hastings N, Law A, McKay S, Marques E, Prasad A, Yu J, Moore SS, Floriot S, Mah? MF, Eggen A, Silveri L, Negrini R, Milanesi E, Ajmone-Marsan P, Valentini A, Marchitelli C, Savarese MC, Janitz M, Herwig R, Hennig S, Gorni C, Connor EE, Sonstegard TS, Smith T, Dr?gem?ller C, Williams JL
BMC genomics7283(2006 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ZBTB4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZBTB4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226522	XM_024980389.1 Latest version!	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa226521	NM_001206898.2 Latest version!	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa226521	XM_005220241.4 Latest version!	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa226521	XM_005220243.4 Latest version!	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa29662	NM_001206898.1 Latest version!	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa29662	XM_005220241.3 Latest version!	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa29662	XM_005220243.3 Latest version!	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZBTB4 ( XM_024980389.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836157.1 Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa226522
Clone ID Related Accession (Same CDS sequence)	XM_024980389.1
Accession Version	XM_024980389.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	zinc finger and BTB domain-containing protein 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGTTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCCTCTC CCTCTCCAGC TTCACCTTCA 
GCTTCATCCC CACCTCGGGT CCTGGAGCTG CCTGGGGTCC CAGCAGCTGC CTTCTCTGAT 
GTCCTCAATT TCATCTACAG TGCCCGGCTG GCATTGCCTG GTGGTGGAGG GGACGGGGCA 
GCTGTGGCAG AGATCGGTGC TCTGGGACGG CGTCTGGGCA TCTCCCGCCT GCAGGGCCTG 
GGGGAGGGGG GTGATGCCTG GGTGCCTCCT GCTCCAGTTC CCATGGCCAC CTCACAGCCT 
GAGGAGGACA GCTTTGGGCC TGGGCCTAGG CCAGCTGGGG AGTGGGAGGG GGACAGGGCT 
GAGGCCCAGG GCCCTGACTC ACAGCCTGCC TTGTCCCGGC GGCCCCTCCC CTGCCCCCGA 
TGTGGGAAAA GCTTCATCCA TCCCAAGCGG CTACAGACCC ATGAGGCCCA GTGCCGGAGG 
GAGGCCAGCG CTCGGGGGTC CGCAGGGCTG GGAGCCAGGG GTTCTGTCCC CAGTGGCCCT 
GCAGGAGTGG ACGCCTCAGC CCTGCCCCCA GCGGTAGGCT TCCGAGGTGG TCCTGAGCAC 
GTGGTGAAGG TGGTGGGTGG CCACGTGCTC TATGTGTGCG CGGCCTGTGA GCGTTCCTAC 
GTGACCCTGT CCAGCCTAAA GCGGCACAGC AACGTACACT CATGGCGGAG GAAGTACCCC 
TGCCGCTACT GCGAGAAGGT GTTCGCACTG GCTGAGTACC GAACCAAACA TGAGGTGTGG 
CACACCGGGG AGCGCAGGTA CCAGTGCATC TTCTGCTGGG AGACCTTTGT CACTTACTAT 
AACCTGAAGA CCCACCAGCG AGCCTTCCAC GGCATTAGCC CAAGCCTCCT GGCCAGCGAG 
AAGACGCCCA ATGGAGGCTA TAAGCCCAAG CTCAATACCC TCAAGCTGTA CCGCCTGCTC 
CCCATGCGGG CGGCCAAGCG GCCCTACAAG ACCTACAGCC AGGGAGCCCC CGAGGCCCCC 
CTCTCTCCAA GCCTCAACAC AGCGGCCCCT GTGGCAATGC CAGCCAGCTC GCCACCCGGA 
CCCGCACCTG CCCCAGAGCC TGGCCCCCCA CCATCTGTCA TCACTTTTGC CCACCCGGCT 
CCCTCAGTCA TTGTACATGG GGGCAGCAGC AGTGGTGGAG CAGGGAGTGG GCCGGCCAGC 
ACAGGGGGGG CCCAAGCCGC CTCAGTCATC ACTTACACTG CTCCCCCACG GCCCCCCAAA 
AAACGTGAGT ACCCACCTCC TCCCCCTGAA CCTGCAGCCG CACCAACCAG CCCAGCCACA 
GCAGGCAGCC CAGCCACAGC CGCAGGGCCC GCCACAGCCA TGGAGGAGGC CAAGGGCCGG 
AACCCACGGG CCACGAGGAC TCTGACCTAC ACGGCCAAGC CAGCTGGCGG GATTGGCGGG 
GGCGCGGGTC CCCCTGCAGG GCCTGGCCGG GGCCCCTCTC AGCTCCAGGC TCCACCGCCA 
CTGTGTCAGA TCACTGTGCG AATCGGTGAG GAGGCGATTG TCAAGCGCCG CATCTCAGAA 
ACAGACCTGC GTCCCGGGGA GCTAAGCGGC GAGGAGATGG AGGAGAGCGA GGAGGAGGAG 
GAGGACGAGG AAGAGGAAGA GGAGGAGGAG GAGGAGGGGC AGGGGGAGTC CAAGGCTGGC 
GGGGAGGACC AGCTCTGGAG GCCCTACTAC TCCTACAAGC CCAAGCGCAA GGCTGGAGCC 
GTGGCCCCGG CTAGCAGCGG GGGCAGCGGG ATGCCCAGAA GCCGCCGGCC ACCTCGCTGG 
AGACAGAAGC TGGAACGGAG GAGCTGGGAG GAGACCCCAG CAGCCGACGG CCCCACGGGG 
CGTGCCCGTG GCGAGAGGAG ACACCGCTGT GAGGACTGTG CCCAGACATT TGCCAGCCTG 
AGGAAGCTGC GCAAGCACCA GGAGTCCCAC AGCGGGGGCC CCCACAGCTC CCGGTCTGGA 
CGGAAGCCCT CCACCCGCCT CACCTGCCCT CACTGCGCCA AGGTGTGCAA GACGGCCGCT 
GCCCTGAGCC GCCATGGGCA GAGGCACGCC GCCGAGCGAC CCGGGGGCAC CCCCACGCCT 
GTCATTGCCT ACTCAAAGGG CAGCACTGGT GCCAGAGCCG GGGAGATTAA GGAGGAGGCC 
CCCCAAGAGA TGCAGGTCTC TTCCTCCAGC GGGGAGGCAG GTGGTGGAGG CGGGAGTGCT 
CCTGCTGAGG AAGCTTCTGA GCCTGCCTCG CTCCAGGACC CTGTCATCTC AGGAGGGGAG 
GAGCCCTCCG TGGGGGCAGG AGGGGCCACC TATGCATACC CACCTGTGCA GGAATTTCCA 
CTGGCTCTGA TTGGGAGCGG CCGGGAATCA GGCAGTGGCA GGGGAAAAGG TGGGAGTGAG 
GGGCCAGGTG GGGCTGGCGG GGGAGACCGG ATGGAGGCGA TGGGAGCTGC CAAAGTCACC 
TTCTACCCTG AGCCCTACCC ACTCGTCTAT GGTCCCCAGC TCCTCGCCGC CTACCCTTAC 
AACTTCAGCA ACCTGGCCGC TCTCCCGGTC GCTCTTAACA TGGTCCTACC TGATGAGAAG 
GGTGGGGGGG CCCTTCCCTT CCTACCAGGG GTCTTTGGCT ACGCAGTCAA TCCTCAAGCA 
GCAGCCCCTA CCCCCCCAAC CCCACCACCC CCAACTCTTC CTCCACCGGT TGCCCCCAAG 
GGAGAAGGGG AGAGGGCAGG GGTTGAAAGA ACCCAGAAGG GAGATGTGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836157.1
CDS	209..3202
Translation	MPPPAEVTDPSHAPAVLRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLASAPLPLPPITGGSAPNPTTTTAASSSSSSSSSPSPASPSASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPPAPVPMATSQPEEDSFGPGPRPAGEWEGDRAEAQGPDSQPALSRRPLPCPRCGKSFIHPKRLQTHEAQCRREASARGSAGLGARGSVPSGPAGVDASALPPAVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRTKHEVWHTGERRYQCIFCWETFVTYYNLKTHQRAFHGISPSLLASEKTPNGGYKPKLNTLKLYRLLPMRAAKRPYKTYSQGAPEAPLSPSLNTAAPVAMPASSPPGPAPAPEPGPPPSVITFAHPAPSVIVHGGSSSGGAGSGPASTGGAQAASVITYTAPPRPPKKREYPPPPPEPAAAPTSPATAGSPATAAGPATAMEEAKGRNPRATRTLTYTAKPAGGIGGGAGPPAGPGRGPSQLQAPPPLCQITVRIGEEAIVKRRISETDLRPGELSGEEMEESEEEEEDEEEEEEEEEEGQGESKAGGEDQLWRPYYSYKPKRKAGAVAPASSGGSGMPRSRRPPRWRQKLERRSWEETPAADGPTGRARGERRHRCEDCAQTFASLRKLRKHQESHSGGPHSSRSGRKPSTRLTCPHCAKVCKTAAALSRHGQRHAAERPGGTPTPVIAYSKGSTGARAGEIKEEAPQEMQVSSSSGEAGGGGGSAPAEEASEPASLQDPVISGGEEPSVGAGGATYAYPPVQEFPLALIGSGRESGSGRGKGGSEGPGGAGGGDRMEAMGAAKVTFYPEPYPLVYGPQLLAAYPYNFSNLAALPVALNMVLPDEKGGGALPFLPGVFGYAVNPQAAAPTPPTPPPPTLPPPVAPKGEGERAGVERTQKGDVG

Target ORF information:

RefSeq Version	XM_024980389.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024980389.1

ORF Insert Sequence:

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGTTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCCTCTC CCTCTCCAGC TTCACCTTCA 
GCTTCATCCC CACCTCGGGT CCTGGAGCTG CCTGGGGTCC CAGCAGCTGC CTTCTCTGAT 
GTCCTCAATT TCATCTACAG TGCCCGGCTG GCATTGCCTG GTGGTGGAGG GGACGGGGCA 
GCTGTGGCAG AGATCGGTGC TCTGGGACGG CGTCTGGGCA TCTCCCGCCT GCAGGGCCTG 
GGGGAGGGGG GTGATGCCTG GGTGCCTCCT GCTCCAGTTC CCATGGCCAC CTCACAGCCT 
GAGGAGGACA GCTTTGGGCC TGGGCCTAGG CCAGCTGGGG AGTGGGAGGG GGACAGGGCT 
GAGGCCCAGG GCCCTGACTC ACAGCCTGCC TTGTCCCGGC GGCCCCTCCC CTGCCCCCGA 
TGTGGGAAAA GCTTCATCCA TCCCAAGCGG CTACAGACCC ATGAGGCCCA GTGCCGGAGG 
GAGGCCAGCG CTCGGGGGTC CGCAGGGCTG GGAGCCAGGG GTTCTGTCCC CAGTGGCCCT 
GCAGGAGTGG ACGCCTCAGC CCTGCCCCCA GCGGTAGGCT TCCGAGGTGG TCCTGAGCAC 
GTGGTGAAGG TGGTGGGTGG CCACGTGCTC TATGTGTGCG CGGCCTGTGA GCGTTCCTAC 
GTGACCCTGT CCAGCCTAAA GCGGCACAGC AACGTACACT CATGGCGGAG GAAGTACCCC 
TGCCGCTACT GCGAGAAGGT GTTCGCACTG GCTGAGTACC GAACCAAACA TGAGGTGTGG 
CACACCGGGG AGCGCAGGTA CCAGTGCATC TTCTGCTGGG AGACCTTTGT CACTTACTAT 
AACCTGAAGA CCCACCAGCG AGCCTTCCAC GGCATTAGCC CAAGCCTCCT GGCCAGCGAG 
AAGACGCCCA ATGGAGGCTA TAAGCCCAAG CTCAATACCC TCAAGCTGTA CCGCCTGCTC 
CCCATGCGGG CGGCCAAGCG GCCCTACAAG ACCTACAGCC AGGGAGCCCC CGAGGCCCCC 
CTCTCTCCAA GCCTCAACAC AGCGGCCCCT GTGGCAATGC CAGCCAGCTC GCCACCCGGA 
CCCGCACCTG CCCCAGAGCC TGGCCCCCCA CCATCTGTCA TCACTTTTGC CCACCCGGCT 
CCCTCAGTCA TTGTACATGG GGGCAGCAGC AGTGGTGGAG CAGGGAGTGG GCCGGCCAGC 
ACAGGGGGGG CCCAAGCCGC CTCAGTCATC ACTTACACTG CTCCCCCACG GCCCCCCAAA 
AAACGTGAGT ACCCACCTCC TCCCCCTGAA CCTGCAGCCG CACCAACCAG CCCAGCCACA 
GCAGGCAGCC CAGCCACAGC CGCAGGGCCC GCCACAGCCA TGGAGGAGGC CAAGGGCCGG 
AACCCACGGG CCACGAGGAC TCTGACCTAC ACGGCCAAGC CAGCTGGCGG GATTGGCGGG 
GGCGCGGGTC CCCCTGCAGG GCCTGGCCGG GGCCCCTCTC AGCTCCAGGC TCCACCGCCA 
CTGTGTCAGA TCACTGTGCG AATCGGTGAG GAGGCGATTG TCAAGCGCCG CATCTCAGAA 
ACAGACCTGC GTCCCGGGGA GCTAAGCGGC GAGGAGATGG AGGAGAGCGA GGAGGAGGAG 
GAGGACGAGG AAGAGGAAGA GGAGGAGGAG GAGGAGGGGC AGGGGGAGTC CAAGGCTGGC 
GGGGAGGACC AGCTCTGGAG GCCCTACTAC TCCTACAAGC CCAAGCGCAA GGCTGGAGCC 
GTGGCCCCGG CTAGCAGCGG GGGCAGCGGG ATGCCCAGAA GCCGCCGGCC ACCTCGCTGG 
AGACAGAAGC TGGAACGGAG GAGCTGGGAG GAGACCCCAG CAGCCGACGG CCCCACGGGG 
CGTGCCCGTG GCGAGAGGAG ACACCGCTGT GAGGACTGTG CCCAGACATT TGCCAGCCTG 
AGGAAGCTGC GCAAGCACCA GGAGTCCCAC AGCGGGGGCC CCCACAGCTC CCGGTCTGGA 
CGGAAGCCCT CCACCCGCCT CACCTGCCCT CACTGCGCCA AGGTGTGCAA GACGGCCGCT 
GCCCTGAGCC GCCATGGGCA GAGGCACGCC GCCGAGCGAC CCGGGGGCAC CCCCACGCCT 
GTCATTGCCT ACTCAAAGGG CAGCACTGGT GCCAGAGCCG GGGAGATTAA GGAGGAGGCC 
CCCCAAGAGA TGCAGGTCTC TTCCTCCAGC GGGGAGGCAG GTGGTGGAGG CGGGAGTGCT 
CCTGCTGAGG AAGCTTCTGA GCCTGCCTCG CTCCAGGACC CTGTCATCTC AGGAGGGGAG 
GAGCCCTCCG TGGGGGCAGG AGGGGCCACC TATGCATACC CACCTGTGCA GGAATTTCCA 
CTGGCTCTGA TTGGGAGCGG CCGGGAATCA GGCAGTGGCA GGGGAAAAGG TGGGAGTGAG 
GGGCCAGGTG GGGCTGGCGG GGGAGACCGG ATGGAGGCGA TGGGAGCTGC CAAAGTCACC 
TTCTACCCTG AGCCCTACCC ACTCGTCTAT GGTCCCCAGC TCCTCGCCGC CTACCCTTAC 
AACTTCAGCA ACCTGGCCGC TCTCCCGGTC GCTCTTAACA TGGTCCTACC TGATGAGAAG 
GGTGGGGGGG CCCTTCCCTT CCTACCAGGG GTCTTTGGCT ACGCAGTCAA TCCTCAAGCA 
GCAGCCCCTA CCCCCCCAAC CCCACCACCC CCAACTCTTC CTCCACCGGT TGCCCCCAAG 
GGAGAAGGGG AGAGGGCAGG GGTTGAAAGA ACCCAGAAGG GAGATGTGGG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZBTB4 ( NM_001206898.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193827.2 Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), mRNA.

Price & Availability↑

CloneID	OBa226521
Clone ID Related Accession (Same CDS sequence)	XM_005220243.4 , NM_001206898.2 , XM_005220241.4
Accession Version	NM_001206898.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-19
Organism	Bos taurus(cattle)
Product	zinc finger and BTB domain-containing protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000019.1. On Dec 20, 2018 this sequence version replaced NM_001206898.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on homologous protein alignment with human NP_065950.2. ##Evidence-Data-START## Transcript exon combination :: SRR5765710.4466.1, SRR5721695.4196.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGTTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACATGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193827.2
CDS	1..3030
Translation	MPPPAEVTDPSHAPAVLRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLASAPLPLPPITGGSAPNPTTTTAASSSSSSSSSSSSSSSSSSSSSPSPASPSASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPPAPVPMATSQPEEDSFGPGPRPAGEWEGDRAEAQGPDSQPALSRRPLPCPRCGKSFIHPKRLQTHEAQCRREASARGSAGLGARGSVPSGPAGVDASALPPAVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRTKHEVWHTGERRYQCIFCWETFVTYYNLKTHQRAFHGISPSLLASEKTPNGGYKPKLNTLKLYRLLPMRAAKRPYKTYSQGAPEAPLSPSLNTAAPVAMPASSPPGPAPAPEPGPPPSVITFAHPAPSVIVHGGSSSGGAGSGPASTGGAQAASVITYTAPPRPPKKREYPPPPPEPAAAPTSPATAGSPATAAGPATAMEEAKGRNPRATRTLTYTAKPAGGIGGGAGPPAGPGRGPSQLQAPPPLCQITVRIGEEAIVKRRISETDLRPGELSGEEMEESEEEEEDEEEEEEEEEEGQGESKAGGEDQLWRPYYSYKPKRKAGAVAPASSGGSGMPRSRRPPRWRQKLERRSWEETPAADGPTGRARGERRHRCEDCAQTFASLRKLRKHQESHSGGPHSSRSGRKPSTRLTCPHCAKVCKTAAALSRHGQRHAAERPGGTPTPVIAYSKGSTGARAGEIKEEAPQEMQVSSSSGEAGGGGGSAPAEEASEPASLQDPVISGGEEPSVGAGGATYAYPPVQEFPLALIGSGRESGSGRGKGGSEGPGGAGGGDRMEAMGAAKVTFYPEPYPLVYGPQLLAAYPYNFSNLAALPVALNMVLPDEKGGGALPFLPGVFGYAVNPQAAAPTPPTPPPPTLPPPVAPKGEGERAGVERTQKGDVG

Target ORF information:

RefSeq Version	NM_001206898.2
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206898.2

ORF Insert Sequence:

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGTTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACATGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZBTB4 ( XM_005220243.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220300.2 Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa226521
Clone ID Related Accession (Same CDS sequence)	XM_005220243.4 , NM_001206898.2 , XM_005220241.4
Accession Version	XM_005220243.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	zinc finger and BTB domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220243.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGTTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACATGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220300.2
CDS	227..3256
Translation	MPPPAEVTDPSHAPAVLRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLASAPLPLPPITGGSAPNPTTTTAASSSSSSSSSSSSSSSSSSSSSPSPASPSASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPPAPVPMATSQPEEDSFGPGPRPAGEWEGDRAEAQGPDSQPALSRRPLPCPRCGKSFIHPKRLQTHEAQCRREASARGSAGLGARGSVPSGPAGVDASALPPAVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRTKHEVWHTGERRYQCIFCWETFVTYYNLKTHQRAFHGISPSLLASEKTPNGGYKPKLNTLKLYRLLPMRAAKRPYKTYSQGAPEAPLSPSLNTAAPVAMPASSPPGPAPAPEPGPPPSVITFAHPAPSVIVHGGSSSGGAGSGPASTGGAQAASVITYTAPPRPPKKREYPPPPPEPAAAPTSPATAGSPATAAGPATAMEEAKGRNPRATRTLTYTAKPAGGIGGGAGPPAGPGRGPSQLQAPPPLCQITVRIGEEAIVKRRISETDLRPGELSGEEMEESEEEEEDEEEEEEEEEEGQGESKAGGEDQLWRPYYSYKPKRKAGAVAPASSGGSGMPRSRRPPRWRQKLERRSWEETPAADGPTGRARGERRHRCEDCAQTFASLRKLRKHQESHSGGPHSSRSGRKPSTRLTCPHCAKVCKTAAALSRHGQRHAAERPGGTPTPVIAYSKGSTGARAGEIKEEAPQEMQVSSSSGEAGGGGGSAPAEEASEPASLQDPVISGGEEPSVGAGGATYAYPPVQEFPLALIGSGRESGSGRGKGGSEGPGGAGGGDRMEAMGAAKVTFYPEPYPLVYGPQLLAAYPYNFSNLAALPVALNMVLPDEKGGGALPFLPGVFGYAVNPQAAAPTPPTPPPPTLPPPVAPKGEGERAGVERTQKGDVG

Target ORF information:

RefSeq Version	XM_005220243.4
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220243.4

ORF Insert Sequence:

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGTTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACATGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZBTB4 ( XM_005220241.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220298.2 Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa226521
Clone ID Related Accession (Same CDS sequence)	XM_005220243.4 , NM_001206898.2 , XM_005220241.4
Accession Version	XM_005220241.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	zinc finger and BTB domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220241.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGTTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACATGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220298.2
CDS	209..3238
Translation	MPPPAEVTDPSHAPAVLRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLASAPLPLPPITGGSAPNPTTTTAASSSSSSSSSSSSSSSSSSSSSPSPASPSASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPPAPVPMATSQPEEDSFGPGPRPAGEWEGDRAEAQGPDSQPALSRRPLPCPRCGKSFIHPKRLQTHEAQCRREASARGSAGLGARGSVPSGPAGVDASALPPAVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRTKHEVWHTGERRYQCIFCWETFVTYYNLKTHQRAFHGISPSLLASEKTPNGGYKPKLNTLKLYRLLPMRAAKRPYKTYSQGAPEAPLSPSLNTAAPVAMPASSPPGPAPAPEPGPPPSVITFAHPAPSVIVHGGSSSGGAGSGPASTGGAQAASVITYTAPPRPPKKREYPPPPPEPAAAPTSPATAGSPATAAGPATAMEEAKGRNPRATRTLTYTAKPAGGIGGGAGPPAGPGRGPSQLQAPPPLCQITVRIGEEAIVKRRISETDLRPGELSGEEMEESEEEEEDEEEEEEEEEEGQGESKAGGEDQLWRPYYSYKPKRKAGAVAPASSGGSGMPRSRRPPRWRQKLERRSWEETPAADGPTGRARGERRHRCEDCAQTFASLRKLRKHQESHSGGPHSSRSGRKPSTRLTCPHCAKVCKTAAALSRHGQRHAAERPGGTPTPVIAYSKGSTGARAGEIKEEAPQEMQVSSSSGEAGGGGGSAPAEEASEPASLQDPVISGGEEPSVGAGGATYAYPPVQEFPLALIGSGRESGSGRGKGGSEGPGGAGGGDRMEAMGAAKVTFYPEPYPLVYGPQLLAAYPYNFSNLAALPVALNMVLPDEKGGGALPFLPGVFGYAVNPQAAAPTPPTPPPPTLPPPVAPKGEGERAGVERTQKGDVG

Target ORF information:

RefSeq Version	XM_005220241.4
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220241.4

ORF Insert Sequence:

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGTTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACATGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZBTB4 ( NM_001206898.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001193827.1 Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), mRNA.

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CloneID	OBa29662
Clone ID Related Accession (Same CDS sequence)	XM_005220241.3 , NM_001206898.1 , XM_005220243.3
Accession Version	NM_001206898.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	zinc finger and BTB domain-containing protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02048776.1, DN521849.1, DY119806.1, DT817601.1, DV820067.1, EH170937.1, DV908241.1 and CO883962.1. On or before May 12, 2011 this sequence version replaced XM_002695788.1, XM_001788701.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on homologous protein alignment with human NP_065950.2. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02403658, SAMN02822088 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGCTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACACGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001193827.1
CDS	1..3030
Translation	MPPPAEVTDPSHAPAALRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLASAPLPLPPITGGSAPNPTTTTAASSSSSSSSSSSSSSSSSSSSSPSPASPSASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPPAPVPMATSQPEEDSFGPGPRPAGEWEGDRAEAQGPDSQPALSRRPLPCPRCGKSFIHPKRLQTHEAQCRREASARGSAGLGARGSVPSGPAGVDASALPPAVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRTKHEVWHTGERRYQCIFCWETFVTYYNLKTHQRAFHGISPSLLASEKTPNGGYKPKLNTLKLYRLLPMRAAKRPYKTYSQGAPEAPLSPSLNTAAPVAMPASSPPGPAPAPEPGPPPSVITFAHPAPSVIVHGGSSSGGAGSGPASTGGAQAASVITYTAPPRPPKKREYPPPPPEPAAAPTSPATAGSPATAAGPATAMEEAKGRNPRATRTLTYTAKPAGGIGGGAGPPAGPGRGPSQLQAPPPLCQITVRIGEEAIVKRRISETDLRPGELSGEEMEESEEEEEDEEEEEEEEEEGQGESKAGGEDQLWRPYYSYKPKRKAGAVAPASSGGSGMPRSRRPPRWRQKLERRSWEETPAADGPTGRARGERRHRCEDCAQTFASLRKLRKHQESHSGGPHSSRSGRKPSTRLTCPHCAKVCKTAAALSRHGQRHAAERPGGTPTPVIAYSKGSTGARAGEIKEEAPQEMQVSSSSGEAGGGGGSAPAEEASEPASLQDPVISGGEEPSVGAGGATYAYPPVQEFPLALIGSGRESGSGRGKGGSEGPGGAGGGDRMEAMGAAKVTFYPEPYPLVYGPQLLAAYPYNFSNLAALPVALNMVLPDEKGGGALPFLPGVFGYAVNPQAAAPTPPTPPPPTLPPPVAPKGEGERAGVERTQKGDVG

Target ORF information:

RefSeq Version	NM_001206898.1
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001206898.1

ORF Insert Sequence:

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGCTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACACGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZBTB4 ( XM_005220241.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220298.1 Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29662
Clone ID Related Accession (Same CDS sequence)	XM_005220241.3 , NM_001206898.1 , XM_005220243.3
Accession Version	XM_005220241.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	zinc finger and BTB domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220241.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGCTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACACGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220298.1
CDS	203..3232
Translation	MPPPAEVTDPSHAPAALRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLASAPLPLPPITGGSAPNPTTTTAASSSSSSSSSSSSSSSSSSSSSPSPASPSASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPPAPVPMATSQPEEDSFGPGPRPAGEWEGDRAEAQGPDSQPALSRRPLPCPRCGKSFIHPKRLQTHEAQCRREASARGSAGLGARGSVPSGPAGVDASALPPAVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRTKHEVWHTGERRYQCIFCWETFVTYYNLKTHQRAFHGISPSLLASEKTPNGGYKPKLNTLKLYRLLPMRAAKRPYKTYSQGAPEAPLSPSLNTAAPVAMPASSPPGPAPAPEPGPPPSVITFAHPAPSVIVHGGSSSGGAGSGPASTGGAQAASVITYTAPPRPPKKREYPPPPPEPAAAPTSPATAGSPATAAGPATAMEEAKGRNPRATRTLTYTAKPAGGIGGGAGPPAGPGRGPSQLQAPPPLCQITVRIGEEAIVKRRISETDLRPGELSGEEMEESEEEEEDEEEEEEEEEEGQGESKAGGEDQLWRPYYSYKPKRKAGAVAPASSGGSGMPRSRRPPRWRQKLERRSWEETPAADGPTGRARGERRHRCEDCAQTFASLRKLRKHQESHSGGPHSSRSGRKPSTRLTCPHCAKVCKTAAALSRHGQRHAAERPGGTPTPVIAYSKGSTGARAGEIKEEAPQEMQVSSSSGEAGGGGGSAPAEEASEPASLQDPVISGGEEPSVGAGGATYAYPPVQEFPLALIGSGRESGSGRGKGGSEGPGGAGGGDRMEAMGAAKVTFYPEPYPLVYGPQLLAAYPYNFSNLAALPVALNMVLPDEKGGGALPFLPGVFGYAVNPQAAAPTPPTPPPPTLPPPVAPKGEGERAGVERTQKGDVG

Target ORF information:

RefSeq Version	XM_005220241.3
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220241.3

ORF Insert Sequence:

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGCTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACACGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZBTB4 ( XM_005220243.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005220300.1 Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X2, mRNA.

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CloneID	OBa29662
Clone ID Related Accession (Same CDS sequence)	XM_005220241.3 , NM_001206898.1 , XM_005220243.3
Accession Version	XM_005220243.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	zinc finger and BTB domain-containing protein 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104493.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220243.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGCTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACACGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005220300.1
CDS	214..3243
Translation	MPPPAEVTDPSHAPAALRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLASAPLPLPPITGGSAPNPTTTTAASSSSSSSSSSSSSSSSSSSSSPSPASPSASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPPAPVPMATSQPEEDSFGPGPRPAGEWEGDRAEAQGPDSQPALSRRPLPCPRCGKSFIHPKRLQTHEAQCRREASARGSAGLGARGSVPSGPAGVDASALPPAVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRTKHEVWHTGERRYQCIFCWETFVTYYNLKTHQRAFHGISPSLLASEKTPNGGYKPKLNTLKLYRLLPMRAAKRPYKTYSQGAPEAPLSPSLNTAAPVAMPASSPPGPAPAPEPGPPPSVITFAHPAPSVIVHGGSSSGGAGSGPASTGGAQAASVITYTAPPRPPKKREYPPPPPEPAAAPTSPATAGSPATAAGPATAMEEAKGRNPRATRTLTYTAKPAGGIGGGAGPPAGPGRGPSQLQAPPPLCQITVRIGEEAIVKRRISETDLRPGELSGEEMEESEEEEEDEEEEEEEEEEGQGESKAGGEDQLWRPYYSYKPKRKAGAVAPASSGGSGMPRSRRPPRWRQKLERRSWEETPAADGPTGRARGERRHRCEDCAQTFASLRKLRKHQESHSGGPHSSRSGRKPSTRLTCPHCAKVCKTAAALSRHGQRHAAERPGGTPTPVIAYSKGSTGARAGEIKEEAPQEMQVSSSSGEAGGGGGSAPAEEASEPASLQDPVISGGEEPSVGAGGATYAYPPVQEFPLALIGSGRESGSGRGKGGSEGPGGAGGGDRMEAMGAAKVTFYPEPYPLVYGPQLLAAYPYNFSNLAALPVALNMVLPDEKGGGALPFLPGVFGYAVNPQAAAPTPPTPPPPTLPPPVAPKGEGERAGVERTQKGDVG

Target ORF information:

RefSeq Version	XM_005220243.3
Organism	Bos taurus(cattle)
Definition	Bos taurus zinc finger and BTB domain containing 4 (ZBTB4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220243.3

ORF Insert Sequence:

ATGCCACCCC CAGCAGAGGT GACAGACCCA TCCCATGCGC CCGCCGCTCT GCGCCAGCTC 
AATGAACAGC GGCTTCGCGG CCTCTTCTGT GACGTCACCC TCATAGCCGG AGATACCAAG 
TTCCCCGCTC ACCGCAGCGT TCTGGCTGCT TCTAGTCCCT TCTTCAGAGA GGCACTGCTG 
GCTTCAGCTC CACTGCCCCT CCCACCCATC ACTGGGGGCT CAGCCCCCAA CCCCACCACC 
ACCACAGCGG CCTCCTCCTC CTCCTCTTCC TCCTCTTCCT CTTCCTCCTC CTCCTCCTCC 
TCTTCCTCCT CCTCTCCCTC TCCAGCTTCA CCTTCAGCTT CATCCCCACC TCGGGTCCTG 
GAGCTGCCTG GGGTCCCAGC AGCTGCCTTC TCTGATGTCC TCAATTTCAT CTACAGTGCC 
CGGCTGGCAT TGCCTGGTGG TGGAGGGGAC GGGGCAGCTG TGGCAGAGAT CGGTGCTCTG 
GGACGGCGTC TGGGCATCTC CCGCCTGCAG GGCCTGGGGG AGGGGGGTGA TGCCTGGGTG 
CCTCCTGCTC CAGTTCCCAT GGCCACCTCA CAGCCTGAGG AGGACAGCTT TGGGCCTGGG 
CCTAGGCCAG CTGGGGAGTG GGAGGGGGAC AGGGCTGAGG CCCAGGGCCC TGACTCACAG 
CCTGCCTTGT CCCGGCGGCC CCTCCCCTGC CCCCGATGTG GGAAAAGCTT CATCCATCCC 
AAGCGGCTAC AGACCCATGA GGCCCAGTGC CGGAGGGAGG CCAGCGCTCG GGGGTCCGCA 
GGGCTGGGAG CCAGGGGTTC TGTCCCCAGT GGCCCTGCAG GAGTGGACGC CTCAGCCCTG 
CCCCCAGCGG TAGGCTTCCG AGGTGGTCCT GAGCACGTGG TGAAGGTGGT GGGTGGCCAC 
GTGCTCTATG TGTGCGCGGC CTGTGAGCGT TCCTACGTGA CCCTGTCCAG CCTAAAGCGG 
CACAGCAACG TACACTCATG GCGGAGGAAG TACCCCTGCC GCTACTGCGA GAAGGTGTTC 
GCACTGGCTG AGTACCGAAC CAAACACGAG GTGTGGCACA CCGGGGAGCG CAGGTACCAG 
TGCATCTTCT GCTGGGAGAC CTTTGTCACT TACTATAACC TGAAGACCCA CCAGCGAGCC 
TTCCACGGCA TTAGCCCAAG CCTCCTGGCC AGCGAGAAGA CGCCCAATGG AGGCTATAAG 
CCCAAGCTCA ATACCCTCAA GCTGTACCGC CTGCTCCCCA TGCGGGCGGC CAAGCGGCCC 
TACAAGACCT ACAGCCAGGG AGCCCCCGAG GCCCCCCTCT CTCCAAGCCT CAACACAGCG 
GCCCCTGTGG CAATGCCAGC CAGCTCGCCA CCCGGACCCG CACCTGCCCC AGAGCCTGGC 
CCCCCACCAT CTGTCATCAC TTTTGCCCAC CCGGCTCCCT CAGTCATTGT ACATGGGGGC 
AGCAGCAGTG GTGGAGCAGG GAGTGGGCCG GCCAGCACAG GGGGGGCCCA AGCCGCCTCA 
GTCATCACTT ACACTGCTCC CCCACGGCCC CCCAAAAAAC GTGAGTACCC ACCTCCTCCC 
CCTGAACCTG CAGCCGCACC AACCAGCCCA GCCACAGCAG GCAGCCCAGC CACAGCCGCA 
GGGCCCGCCA CAGCCATGGA GGAGGCCAAG GGCCGGAACC CACGGGCCAC GAGGACTCTG 
ACCTACACGG CCAAGCCAGC TGGCGGGATT GGCGGGGGCG CGGGTCCCCC TGCAGGGCCT 
GGCCGGGGCC CCTCTCAGCT CCAGGCTCCA CCGCCACTGT GTCAGATCAC TGTGCGAATC 
GGTGAGGAGG CGATTGTCAA GCGCCGCATC TCAGAAACAG ACCTGCGTCC CGGGGAGCTA 
AGCGGCGAGG AGATGGAGGA GAGCGAGGAG GAGGAGGAGG ACGAGGAAGA GGAAGAGGAG 
GAGGAGGAGG AGGGGCAGGG GGAGTCCAAG GCTGGCGGGG AGGACCAGCT CTGGAGGCCC 
TACTACTCCT ACAAGCCCAA GCGCAAGGCT GGAGCCGTGG CCCCGGCTAG CAGCGGGGGC 
AGCGGGATGC CCAGAAGCCG CCGGCCACCT CGCTGGAGAC AGAAGCTGGA ACGGAGGAGC 
TGGGAGGAGA CCCCAGCAGC CGACGGCCCC ACGGGGCGTG CCCGTGGCGA GAGGAGACAC 
CGCTGTGAGG ACTGTGCCCA GACATTTGCC AGCCTGAGGA AGCTGCGCAA GCACCAGGAG 
TCCCACAGCG GGGGCCCCCA CAGCTCCCGG TCTGGACGGA AGCCCTCCAC CCGCCTCACC 
TGCCCTCACT GCGCCAAGGT GTGCAAGACG GCCGCTGCCC TGAGCCGCCA TGGGCAGAGG 
CACGCCGCCG AGCGACCCGG GGGCACCCCC ACGCCTGTCA TTGCCTACTC AAAGGGCAGC 
ACTGGTGCCA GAGCCGGGGA GATTAAGGAG GAGGCCCCCC AAGAGATGCA GGTCTCTTCC 
TCCAGCGGGG AGGCAGGTGG TGGAGGCGGG AGTGCTCCTG CTGAGGAAGC TTCTGAGCCT 
GCCTCGCTCC AGGACCCTGT CATCTCAGGA GGGGAGGAGC CCTCCGTGGG GGCAGGAGGG 
GCCACCTATG CATACCCACC TGTGCAGGAA TTTCCACTGG CTCTGATTGG GAGCGGCCGG 
GAATCAGGCA GTGGCAGGGG AAAAGGTGGG AGTGAGGGGC CAGGTGGGGC TGGCGGGGGA 
GACCGGATGG AGGCGATGGG AGCTGCCAAA GTCACCTTCT ACCCTGAGCC CTACCCACTC 
GTCTATGGTC CCCAGCTCCT CGCCGCCTAC CCTTACAACT TCAGCAACCT GGCCGCTCTC 
CCGGTCGCTC TTAACATGGT CCTACCTGAT GAGAAGGGTG GGGGGGCCCT TCCCTTCCTA 
CCAGGGGTCT TTGGCTACGC AGTCAATCCT CAAGCAGCAG CCCCTACCCC CCCAACCCCA 
CCACCCCCAA CTCTTCCTCC ACCGGTTGCC CCCAAGGGAG AAGGGGAGAG GGCAGGGGTT 
GAAAGAACCC AGAAGGGAGA TGTGGGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A second generation radiation hybrid map to aid the assembly of the bovine genome sequence.
BMC genomics7283(2006 Nov)
Jann OC,Aerts J,Jones M,Hastings N,Law A,McKay S,Marques E,Prasad A,Yu J,Moore SS,Floriot S,Mah? MF,Eggen A,Silveri L,Negrini R,Milanesi E,Ajmone-Marsan P,Valentini A,Marchitelli C,Savarese MC,Janitz M,Herwig R,Hennig S,Gorni C,Connor EE,Sonstegard TS,Smith T,Dr?gem?ller C,Williams JL

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ZBTB4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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