UPF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015463937.1	XP_015319423.1	regulator of nonsense transcripts 1 isoform X1
XM_002688526.5	XP_002688572.1	regulator of nonsense transcripts 1 isoform X2
XM_010806543.2	XP_010804845.1	regulator of nonsense transcripts 1 isoform X1
XM_015463938.1	XP_015319424.1	regulator of nonsense transcripts 1 isoform X2
XM_015463936.1	XP_015319422.1	regulator of nonsense transcripts 1 isoform X1
XM_005208480.3	XP_005208537.1	regulator of nonsense transcripts 1 isoform X1
XM_005208480.4	XP_005208537.1	regulator of nonsense transcripts 1 isoform X1
XM_010806543.3	XP_010804845.1	regulator of nonsense transcripts 1 isoform X1
XM_002688526.6	XP_002688572.1	regulator of nonsense transcripts 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa236342	XM_010806543.3 Latest version!	Bos taurus UPF1, RNA helicase and ATPase (UPF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa236342	XM_005208480.4 Latest version!	Bos taurus UPF1, RNA helicase and ATPase (UPF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa236343	XM_002688526.6 Latest version!	Bos taurus UPF1, RNA helicase and ATPase (UPF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa20814	XM_015463937.1 Latest version!	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa20814	XM_015463936.1 Latest version!	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa20814	XM_010806543.2 Latest version!	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa20814	XM_005208480.3 Latest version!	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa20815	XM_015463938.1 Latest version!	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa20815	XM_002688526.5 Latest version!	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

UPF1 ( XM_005208480.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208537.1 Bos taurus UPF1, RNA helicase and ATPase (UPF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa236342
Clone ID Related Accession (Same CDS sequence)	XM_005208480.4 , XM_010806543.3
Accession Version	XM_005208480.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208480.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCTC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208537.1
CDS	1..3384
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGAGAPVGAGPGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_005208480.4
Organism	Bos taurus(cattle)
Definition	Bos taurus UPF1, RNA helicase and ATPase (UPF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208480.4

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCTC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_010806543.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804845.1 Bos taurus UPF1, RNA helicase and ATPase (UPF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa236342
Clone ID Related Accession (Same CDS sequence)	XM_005208480.4 , XM_010806543.3
Accession Version	XM_010806543.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806543.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCTC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804845.1
CDS	1..3384
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGAGAPVGAGPGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_010806543.3
Organism	Bos taurus(cattle)
Definition	Bos taurus UPF1, RNA helicase and ATPase (UPF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806543.3

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCTC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_002688526.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002688572.1 Bos taurus UPF1, RNA helicase and ATPase (UPF1), transcript variant X3, mRNA.

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CloneID	OBa236343
Clone ID Related Accession (Same CDS sequence)	XM_002688526.6
Accession Version	XM_002688526.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	regulator of nonsense transcripts 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002688526.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GACATGCGGC TCATGCAAGG GGATGAGATA 
TGCCTGCGGT ACAAAGGGGA CCTGGCGCCC CTCTGGAAAG GGATCGGCCA CGTCATCAAG 
GTCCCTGATA ATTATGGCGA TGAGATTGCT ATTGAGCTTC GGAGCAGCGT GGGCGCCCCT 
GTGGAGGTGA CTCATAACTT CCAGGTGGAT TTCGTGTGGA AGTCAACCTC ATTCGACAGA 
ATGCAGAGCG CACTGAAAAC CTTTGCCGTG GATGAGACCT CCGTGTCGGG CTACATCTAC 
CACAAACTGC TTGGCCATGA GGTGGAGGAT GTGATCATCA AGTGCCAGCT GCCCAAGCGC 
TTCACGGCAC AGGGGCTCCC CGACCTCAAC CACTCTCAGG TTTACGCCGT GAAGACTGTG 
CTGCAGAGAC CACTGAGTCT GATCCAGGGC CCACCGGGCA CAGGAAAGAC CGTGACCTCA 
GCCACCATCG TCTACCACCT GGCTCGGCAA GGCAACGGGC CTGTGCTGGT CTGTGCTCCA 
AGCAACATAG CCGTGGATCA GCTGACTGAG AAGATCCACC AGACCGGGCT GAAAGTCGTG 
CGGCTCTGCG CCAAGAGCCG CGAGGCCATA GACTCCCCGG TGTCCTTCTT GGCCCTGCAC 
AACCAGATCA GGAACATGGA CAGCATGCCA GAGCTCCAAA AGCTGCAGCA GCTGAAGGAT 
GAGACTGGGG AGCTGTCGTC GGCGGACGAG AAGCGTTACA GAGCCCTGAA GCGCACTGCC 
GAGAGGGAGC TGCTCATGAA CGCAGATGTC ATCTGCTGCA CGTGTGTGGG CGCAGGAGAC 
CCGAGGCTCG CCAAGATGCA GTTCCGCTCC ATTTTAATCG ACGAAAGCAC GCAGGCCACC 
GAGCCAGAGT GCATGGTCCC TGTGGTCCTT GGAGCCAAGC AGCTGATCCT CGTGGGTGAC 
CACTGCCAGC TGGGCCCTGT GGTGATGTGC AAGAAGGCGG CCAAGGCCGG GCTGTCGCAG 
TCGCTCTTTG AACGCCTGGT GGTGCTGGGC ATCCGCCCCA TCCGCCTCCA GGTCCAGTAC 
CGGATGCACC CCGCGCTCAG CGCCTTCCCG TCCAACATCT TCTACGAGGG CTCTCTCCAG 
AATGGCGTTA CCGCAGCGGA TCGTGTAAAG AAGGGGTTTG ACTTTCAGTG GCCCCAACCG 
GATAAACCAA TGTTCTTCTA TGTGACCCAG GGCCAAGAGG AGATTGCCAG CTCAGGCACC 
TCCTACCTGA ACAGGACGGA GGCTGCAAAC GTGGAGAAGA TCACCACGAA GCTGTTGAAG 
GCGGGCGCCA AGCCGGACCA GATCGGCATC ATCACGCCCT ACGAGGGCCA GCGCTCCTAC 
CTGGTGCAGT ACATGCAGTT CAGCGGCTCC CTGCACACCA AGCTCTACCA GGAGGTGGAG 
ATTGCCAGCG TGGACGCGTT CCAGGGGCGT GAGAAGGACT TTATCATCCT CTCCTGTGTG 
CGGGCCAATG AGCACCAAGG CATTGGATTT TTGAATGACC CCAGGCGACT CAACGTGGCC 
TTGACCAGAG CACGATACGG GGTCATTATC GTGGGCAACC CGAAGGCCCT GTCCAAGCAG 
CCGTTATGGA ACCACCTGCT GAACTACTAC AAGGAGCAGA AGGTGCTGGT GGAGGGACCC 
CTGAACAACC TGCGGGAGAG CCTCATGCAG TTCAGCAAGC CCAGGAAGCT GGTCAACACC 
ATCAACCCGG GAGCCCGCTT CATGACGACA GCCATGTATG ATGCCCGGGA GGCCATCATC 
CCAGGGTCGG TCTATGACCG GAGCAGCCAG GGCCGGCCCT CGAACATGTA CTTCCAGACC 
CACGACCAGA TTGGCATGAT CAGCGCCGGC CCCAGCCACG TGGCTGCCAT GAACATCCCC 
ATTCCCTTCA ACCTGGTCAT GCCGCCCATG CCGCCACCGG GGTATTTTGG ACAAGCTAAT 
GGGCCAGCCG CAGGCCGAGG CACCCCGAAA GGCAAGACTG GTCGTGGGGG GCGCCAGAAG 
AACCGCTTTG GGCTTCCTGG GCCCAGTCAG ACGAATCTCC CCAACAGCCA GGCCAGCCAG 
GATGTGGCGT CCCAGCCCTT CTCCCAGGGC GCCCTGACCC AAGGCTACAT CTCCATGAGC 
CAGCCCTCCC AGATGAGCCA GCCCGGCCTC TCCCAGCCTG AGCTGTCCCA GGACAGCTAC 
CTTGGAGATG AGTTTAAGTC ACAGATCGAC GTGGCGCTGT CGCAAGACTC CACATACCAG 
GGCGAGCGGG CGTACCAGCA CGGTGGAGTG ACGGGGCTGT CCCAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002688572.1
CDS	273..3623
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGAGAPVGAGPGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_002688526.6
Organism	Bos taurus(cattle)
Definition	Bos taurus UPF1, RNA helicase and ATPase (UPF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002688526.6

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GACATGCGGC TCATGCAAGG GGATGAGATA 
TGCCTGCGGT ACAAAGGGGA CCTGGCGCCC CTCTGGAAAG GGATCGGCCA CGTCATCAAG 
GTCCCTGATA ATTATGGCGA TGAGATTGCT ATTGAGCTTC GGAGCAGCGT GGGCGCCCCT 
GTGGAGGTGA CTCATAACTT CCAGGTGGAT TTCGTGTGGA AGTCAACCTC ATTCGACAGA 
ATGCAGAGCG CACTGAAAAC CTTTGCCGTG GATGAGACCT CCGTGTCGGG CTACATCTAC 
CACAAACTGC TTGGCCATGA GGTGGAGGAT GTGATCATCA AGTGCCAGCT GCCCAAGCGC 
TTCACGGCAC AGGGGCTCCC CGACCTCAAC CACTCTCAGG TTTACGCCGT GAAGACTGTG 
CTGCAGAGAC CACTGAGTCT GATCCAGGGC CCACCGGGCA CAGGAAAGAC CGTGACCTCA 
GCCACCATCG TCTACCACCT GGCTCGGCAA GGCAACGGGC CTGTGCTGGT CTGTGCTCCA 
AGCAACATAG CCGTGGATCA GCTGACTGAG AAGATCCACC AGACCGGGCT GAAAGTCGTG 
CGGCTCTGCG CCAAGAGCCG CGAGGCCATA GACTCCCCGG TGTCCTTCTT GGCCCTGCAC 
AACCAGATCA GGAACATGGA CAGCATGCCA GAGCTCCAAA AGCTGCAGCA GCTGAAGGAT 
GAGACTGGGG AGCTGTCGTC GGCGGACGAG AAGCGTTACA GAGCCCTGAA GCGCACTGCC 
GAGAGGGAGC TGCTCATGAA CGCAGATGTC ATCTGCTGCA CGTGTGTGGG CGCAGGAGAC 
CCGAGGCTCG CCAAGATGCA GTTCCGCTCC ATTTTAATCG ACGAAAGCAC GCAGGCCACC 
GAGCCAGAGT GCATGGTCCC TGTGGTCCTT GGAGCCAAGC AGCTGATCCT CGTGGGTGAC 
CACTGCCAGC TGGGCCCTGT GGTGATGTGC AAGAAGGCGG CCAAGGCCGG GCTGTCGCAG 
TCGCTCTTTG AACGCCTGGT GGTGCTGGGC ATCCGCCCCA TCCGCCTCCA GGTCCAGTAC 
CGGATGCACC CCGCGCTCAG CGCCTTCCCG TCCAACATCT TCTACGAGGG CTCTCTCCAG 
AATGGCGTTA CCGCAGCGGA TCGTGTAAAG AAGGGGTTTG ACTTTCAGTG GCCCCAACCG 
GATAAACCAA TGTTCTTCTA TGTGACCCAG GGCCAAGAGG AGATTGCCAG CTCAGGCACC 
TCCTACCTGA ACAGGACGGA GGCTGCAAAC GTGGAGAAGA TCACCACGAA GCTGTTGAAG 
GCGGGCGCCA AGCCGGACCA GATCGGCATC ATCACGCCCT ACGAGGGCCA GCGCTCCTAC 
CTGGTGCAGT ACATGCAGTT CAGCGGCTCC CTGCACACCA AGCTCTACCA GGAGGTGGAG 
ATTGCCAGCG TGGACGCGTT CCAGGGGCGT GAGAAGGACT TTATCATCCT CTCCTGTGTG 
CGGGCCAATG AGCACCAAGG CATTGGATTT TTGAATGACC CCAGGCGACT CAACGTGGCC 
TTGACCAGAG CACGATACGG GGTCATTATC GTGGGCAACC CGAAGGCCCT GTCCAAGCAG 
CCGTTATGGA ACCACCTGCT GAACTACTAC AAGGAGCAGA AGGTGCTGGT GGAGGGACCC 
CTGAACAACC TGCGGGAGAG CCTCATGCAG TTCAGCAAGC CCAGGAAGCT GGTCAACACC 
ATCAACCCGG GAGCCCGCTT CATGACGACA GCCATGTATG ATGCCCGGGA GGCCATCATC 
CCAGGGTCGG TCTATGACCG GAGCAGCCAG GGCCGGCCCT CGAACATGTA CTTCCAGACC 
CACGACCAGA TTGGCATGAT CAGCGCCGGC CCCAGCCACG TGGCTGCCAT GAACATCCCC 
ATTCCCTTCA ACCTGGTCAT GCCGCCCATG CCGCCACCGG GGTATTTTGG ACAAGCTAAT 
GGGCCAGCCG CAGGCCGAGG CACCCCGAAA GGCAAGACTG GTCGTGGGGG GCGCCAGAAG 
AACCGCTTTG GGCTTCCTGG GCCCAGTCAG ACGAATCTCC CCAACAGCCA GGCCAGCCAG 
GATGTGGCGT CCCAGCCCTT CTCCCAGGGC GCCCTGACCC AAGGCTACAT CTCCATGAGC 
CAGCCCTCCC AGATGAGCCA GCCCGGCCTC TCCCAGCCTG AGCTGTCCCA GGACAGCTAC 
CTTGGAGATG AGTTTAAGTC ACAGATCGAC GTGGCGCTGT CGCAAGACTC CACATACCAG 
GGCGAGCGGG CGTACCAGCA CGGTGGAGTG ACGGGGCTGT CCCAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_015463937.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319423.1 Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X2, mRNA.

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CloneID	OBa20814
Clone ID Related Accession (Same CDS sequence)	XM_015463937.1 , XM_010806543.2 , XM_015463936.1 , XM_005208480.3
Accession Version	XM_015463937.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645001.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCGC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319423.1
CDS	1..3384
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGAGAPVGAGPGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_015463937.1
Organism	Bos taurus(cattle)
Definition	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463937.1

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCGC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_010806543.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804845.1 Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X2, mRNA.

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CloneID	OBa20814
Clone ID Related Accession (Same CDS sequence)	XM_015463937.1 , XM_010806543.2 , XM_015463936.1 , XM_005208480.3
Accession Version	XM_010806543.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104001.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806543.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCGC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804845.1
CDS	1..3384
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGAGAPVGAGPGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_010806543.2
Organism	Bos taurus(cattle)
Definition	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010806543.2

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCGC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_015463936.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319422.1 Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20814
Clone ID Related Accession (Same CDS sequence)	XM_015463937.1 , XM_010806543.2 , XM_015463936.1 , XM_005208480.3
Accession Version	XM_015463936.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645001.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCGC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319422.1
CDS	1..3384
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGAGAPVGAGPGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_015463936.1
Organism	Bos taurus(cattle)
Definition	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463936.1

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCGC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_005208480.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005208537.1 Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20814
Clone ID Related Accession (Same CDS sequence)	XM_015463937.1 , XM_010806543.2 , XM_015463936.1 , XM_005208480.3
Accession Version	XM_005208480.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regulator of nonsense transcripts 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104001.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208480.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCGC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005208537.1
CDS	1..3384
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGAGAPVGAGPGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_005208480.3
Organism	Bos taurus(cattle)
Definition	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005208480.3

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GGTAATGAGG ATTTAGTCAT AATTTGGTTA 
AGAGACATGC GGCTCATGCA AGGGGATGAG ATATGCCTGC GGTACAAAGG GGACCTGGCG 
CCCCTCTGGA AAGGGATCGG CCACGTCATC AAGGTCCCTG ATAATTATGG CGATGAGATT 
GCTATTGAGC TTCGGAGCAG CGTGGGCGCC CCTGTGGAGG TGACTCATAA CTTCCAGGTG 
GATTTCGTGT GGAAGTCAAC CTCATTCGAC AGAATGCAGA GCGCACTGAA AACCTTTGCC 
GTGGATGAGA CCTCCGTGTC GGGCTACATC TACCACAAAC TGCTTGGCCA TGAGGTGGAG 
GATGTGATCA TCAAGTGCCA GCTGCCCAAG CGCTTCACGG CACAGGGGCT CCCCGACCTC 
AACCACTCGC AGGTTTACGC CGTGAAGACT GTGCTGCAGA GACCACTGAG TCTGATCCAG 
GGCCCACCGG GCACAGGAAA GACCGTGACC TCAGCCACCA TCGTCTACCA CCTGGCTCGG 
CAAGGCAACG GGCCTGTGCT GGTCTGTGCT CCAAGCAACA TAGCCGTGGA TCAGCTGACT 
GAGAAGATCC ACCAGACCGG GCTGAAAGTC GTGCGGCTCT GCGCCAAGAG CCGCGAGGCC 
ATAGACTCCC CGGTGTCCTT CTTGGCCCTG CACAACCAGA TCAGGAACAT GGACAGCATG 
CCAGAGCTCC AAAAGCTGCA GCAGCTGAAG GATGAGACTG GGGAGCTGTC GTCGGCGGAC 
GAGAAGCGTT ACAGAGCCCT GAAGCGCACT GCCGAGAGGG AGCTGCTCAT GAACGCAGAT 
GTCATCTGCT GCACGTGTGT GGGCGCAGGA GACCCGAGGC TCGCCAAGAT GCAGTTCCGC 
TCCATTTTAA TCGACGAAAG CACGCAGGCC ACCGAGCCAG AGTGCATGGT CCCTGTGGTC 
CTTGGAGCCA AGCAGCTGAT CCTCGTGGGT GACCACTGCC AGCTGGGCCC TGTGGTGATG 
TGCAAGAAGG CGGCCAAGGC CGGGCTGTCG CAGTCGCTCT TTGAACGCCT GGTGGTGCTG 
GGCATCCGCC CCATCCGCCT CCAGGTCCAG TACCGGATGC ACCCCGCGCT CAGCGCCTTC 
CCGTCCAACA TCTTCTACGA GGGCTCTCTC CAGAATGGCG TTACCGCAGC GGATCGTGTA 
AAGAAGGGGT TTGACTTTCA GTGGCCCCAA CCGGATAAAC CAATGTTCTT CTATGTGACC 
CAGGGCCAAG AGGAGATTGC CAGCTCAGGC ACCTCCTACC TGAACAGGAC GGAGGCTGCA 
AACGTGGAGA AGATCACCAC GAAGCTGTTG AAGGCGGGCG CCAAGCCGGA CCAGATCGGC 
ATCATCACGC CCTACGAGGG CCAGCGCTCC TACCTGGTGC AGTACATGCA GTTCAGCGGC 
TCCCTGCACA CCAAGCTCTA CCAGGAGGTG GAGATTGCCA GCGTGGACGC GTTCCAGGGG 
CGTGAGAAGG ACTTTATCAT CCTCTCCTGT GTGCGGGCCA ATGAGCACCA AGGCATTGGA 
TTTTTGAATG ACCCCAGGCG ACTCAACGTG GCCTTGACCA GAGCACGATA CGGGGTCATT 
ATCGTGGGCA ACCCGAAGGC CCTGTCCAAG CAGCCGTTAT GGAACCACCT GCTGAACTAC 
TACAAGGAGC AGAAGGTGCT GGTGGAGGGA CCCCTGAACA ACCTGCGGGA GAGCCTCATG 
CAGTTCAGCA AGCCCAGGAA GCTGGTCAAC ACCATCAACC CGGGAGCCCG CTTCATGACG 
ACAGCCATGT ATGATGCCCG GGAGGCCATC ATCCCAGGGT CGGTCTATGA CCGGAGCAGC 
CAGGGCCGGC CCTCGAACAT GTACTTCCAG ACCCACGACC AGATTGGCAT GATCAGCGCC 
GGCCCCAGCC ACGTGGCTGC CATGAACATC CCCATTCCCT TCAACCTGGT CATGCCGCCC 
ATGCCGCCAC CGGGGTATTT TGGACAAGCT AATGGGCCAG CCGCAGGCCG AGGCACCCCG 
AAAGGCAAGA CTGGTCGTGG GGGGCGCCAG AAGAACCGCT TTGGGCTTCC TGGGCCCAGT 
CAGACGAATC TCCCCAACAG CCAGGCCAGC CAGGATGTGG CGTCCCAGCC CTTCTCCCAG 
GGCGCCCTGA CCCAAGGCTA CATCTCCATG AGCCAGCCCT CCCAGATGAG CCAGCCCGGC 
CTCTCCCAGC CTGAGCTGTC CCAGGACAGC TACCTTGGAG ATGAGTTTAA GTCACAGATC 
GACGTGGCGC TGTCGCAAGA CTCCACATAC CAGGGCGAGC GGGCGTACCA GCACGGTGGA 
GTGACGGGGC TGTCCCAGTA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_002688526.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002688572.1 Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa20815
Clone ID Related Accession (Same CDS sequence)	XM_002688526.5 , XM_015463938.1
Accession Version	XM_002688526.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regulator of nonsense transcripts 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104001.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002688526.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GACATGCGGC TCATGCAAGG GGATGAGATA 
TGCCTGCGGT ACAAAGGGGA CCTGGCGCCC CTCTGGAAAG GGATCGGCCA CGTCATCAAG 
GTCCCTGATA ATTATGGCGA TGAGATTGCT ATTGAGCTTC GGAGCAGCGT GGGCGCCCCT 
GTGGAGGTGA CTCATAACTT CCAGGTGGAT TTCGTGTGGA AGTCAACCTC ATTCGACAGA 
ATGCAGAGCG CACTGAAAAC CTTTGCCGTG GATGAGACCT CCGTGTCGGG CTACATCTAC 
CACAAACTGC TTGGCCATGA GGTGGAGGAT GTGATCATCA AGTGCCAGCT GCCCAAGCGC 
TTCACGGCAC AGGGGCTCCC CGACCTCAAC CACTCGCAGG TTTACGCCGT GAAGACTGTG 
CTGCAGAGAC CACTGAGTCT GATCCAGGGC CCACCGGGCA CAGGAAAGAC CGTGACCTCA 
GCCACCATCG TCTACCACCT GGCTCGGCAA GGCAACGGGC CTGTGCTGGT CTGTGCTCCA 
AGCAACATAG CCGTGGATCA GCTGACTGAG AAGATCCACC AGACCGGGCT GAAAGTCGTG 
CGGCTCTGCG CCAAGAGCCG CGAGGCCATA GACTCCCCGG TGTCCTTCTT GGCCCTGCAC 
AACCAGATCA GGAACATGGA CAGCATGCCA GAGCTCCAAA AGCTGCAGCA GCTGAAGGAT 
GAGACTGGGG AGCTGTCGTC GGCGGACGAG AAGCGTTACA GAGCCCTGAA GCGCACTGCC 
GAGAGGGAGC TGCTCATGAA CGCAGATGTC ATCTGCTGCA CGTGTGTGGG CGCAGGAGAC 
CCGAGGCTCG CCAAGATGCA GTTCCGCTCC ATTTTAATCG ACGAAAGCAC GCAGGCCACC 
GAGCCAGAGT GCATGGTCCC TGTGGTCCTT GGAGCCAAGC AGCTGATCCT CGTGGGTGAC 
CACTGCCAGC TGGGCCCTGT GGTGATGTGC AAGAAGGCGG CCAAGGCCGG GCTGTCGCAG 
TCGCTCTTTG AACGCCTGGT GGTGCTGGGC ATCCGCCCCA TCCGCCTCCA GGTCCAGTAC 
CGGATGCACC CCGCGCTCAG CGCCTTCCCG TCCAACATCT TCTACGAGGG CTCTCTCCAG 
AATGGCGTTA CCGCAGCGGA TCGTGTAAAG AAGGGGTTTG ACTTTCAGTG GCCCCAACCG 
GATAAACCAA TGTTCTTCTA TGTGACCCAG GGCCAAGAGG AGATTGCCAG CTCAGGCACC 
TCCTACCTGA ACAGGACGGA GGCTGCAAAC GTGGAGAAGA TCACCACGAA GCTGTTGAAG 
GCGGGCGCCA AGCCGGACCA GATCGGCATC ATCACGCCCT ACGAGGGCCA GCGCTCCTAC 
CTGGTGCAGT ACATGCAGTT CAGCGGCTCC CTGCACACCA AGCTCTACCA GGAGGTGGAG 
ATTGCCAGCG TGGACGCGTT CCAGGGGCGT GAGAAGGACT TTATCATCCT CTCCTGTGTG 
CGGGCCAATG AGCACCAAGG CATTGGATTT TTGAATGACC CCAGGCGACT CAACGTGGCC 
TTGACCAGAG CACGATACGG GGTCATTATC GTGGGCAACC CGAAGGCCCT GTCCAAGCAG 
CCGTTATGGA ACCACCTGCT GAACTACTAC AAGGAGCAGA AGGTGCTGGT GGAGGGACCC 
CTGAACAACC TGCGGGAGAG CCTCATGCAG TTCAGCAAGC CCAGGAAGCT GGTCAACACC 
ATCAACCCGG GAGCCCGCTT CATGACGACA GCCATGTATG ATGCCCGGGA GGCCATCATC 
CCAGGGTCGG TCTATGACCG GAGCAGCCAG GGCCGGCCCT CGAACATGTA CTTCCAGACC 
CACGACCAGA TTGGCATGAT CAGCGCCGGC CCCAGCCACG TGGCTGCCAT GAACATCCCC 
ATTCCCTTCA ACCTGGTCAT GCCGCCCATG CCGCCACCGG GGTATTTTGG ACAAGCTAAT 
GGGCCAGCCG CAGGCCGAGG CACCCCGAAA GGCAAGACTG GTCGTGGGGG GCGCCAGAAG 
AACCGCTTTG GGCTTCCTGG GCCCAGTCAG ACGAATCTCC CCAACAGCCA GGCCAGCCAG 
GATGTGGCGT CCCAGCCCTT CTCCCAGGGC GCCCTGACCC AAGGCTACAT CTCCATGAGC 
CAGCCCTCCC AGATGAGCCA GCCCGGCCTC TCCCAGCCTG AGCTGTCCCA GGACAGCTAC 
CTTGGAGATG AGTTTAAGTC ACAGATCGAC GTGGCGCTGT CGCAAGACTC CACATACCAG 
GGCGAGCGGG CGTACCAGCA CGGTGGAGTG ACGGGGCTGT CCCAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002688572.1
CDS	1..3351
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGAGAPVGAGPGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_002688526.5
Organism	Bos taurus(cattle)
Definition	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002688526.5

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GACATGCGGC TCATGCAAGG GGATGAGATA 
TGCCTGCGGT ACAAAGGGGA CCTGGCGCCC CTCTGGAAAG GGATCGGCCA CGTCATCAAG 
GTCCCTGATA ATTATGGCGA TGAGATTGCT ATTGAGCTTC GGAGCAGCGT GGGCGCCCCT 
GTGGAGGTGA CTCATAACTT CCAGGTGGAT TTCGTGTGGA AGTCAACCTC ATTCGACAGA 
ATGCAGAGCG CACTGAAAAC CTTTGCCGTG GATGAGACCT CCGTGTCGGG CTACATCTAC 
CACAAACTGC TTGGCCATGA GGTGGAGGAT GTGATCATCA AGTGCCAGCT GCCCAAGCGC 
TTCACGGCAC AGGGGCTCCC CGACCTCAAC CACTCGCAGG TTTACGCCGT GAAGACTGTG 
CTGCAGAGAC CACTGAGTCT GATCCAGGGC CCACCGGGCA CAGGAAAGAC CGTGACCTCA 
GCCACCATCG TCTACCACCT GGCTCGGCAA GGCAACGGGC CTGTGCTGGT CTGTGCTCCA 
AGCAACATAG CCGTGGATCA GCTGACTGAG AAGATCCACC AGACCGGGCT GAAAGTCGTG 
CGGCTCTGCG CCAAGAGCCG CGAGGCCATA GACTCCCCGG TGTCCTTCTT GGCCCTGCAC 
AACCAGATCA GGAACATGGA CAGCATGCCA GAGCTCCAAA AGCTGCAGCA GCTGAAGGAT 
GAGACTGGGG AGCTGTCGTC GGCGGACGAG AAGCGTTACA GAGCCCTGAA GCGCACTGCC 
GAGAGGGAGC TGCTCATGAA CGCAGATGTC ATCTGCTGCA CGTGTGTGGG CGCAGGAGAC 
CCGAGGCTCG CCAAGATGCA GTTCCGCTCC ATTTTAATCG ACGAAAGCAC GCAGGCCACC 
GAGCCAGAGT GCATGGTCCC TGTGGTCCTT GGAGCCAAGC AGCTGATCCT CGTGGGTGAC 
CACTGCCAGC TGGGCCCTGT GGTGATGTGC AAGAAGGCGG CCAAGGCCGG GCTGTCGCAG 
TCGCTCTTTG AACGCCTGGT GGTGCTGGGC ATCCGCCCCA TCCGCCTCCA GGTCCAGTAC 
CGGATGCACC CCGCGCTCAG CGCCTTCCCG TCCAACATCT TCTACGAGGG CTCTCTCCAG 
AATGGCGTTA CCGCAGCGGA TCGTGTAAAG AAGGGGTTTG ACTTTCAGTG GCCCCAACCG 
GATAAACCAA TGTTCTTCTA TGTGACCCAG GGCCAAGAGG AGATTGCCAG CTCAGGCACC 
TCCTACCTGA ACAGGACGGA GGCTGCAAAC GTGGAGAAGA TCACCACGAA GCTGTTGAAG 
GCGGGCGCCA AGCCGGACCA GATCGGCATC ATCACGCCCT ACGAGGGCCA GCGCTCCTAC 
CTGGTGCAGT ACATGCAGTT CAGCGGCTCC CTGCACACCA AGCTCTACCA GGAGGTGGAG 
ATTGCCAGCG TGGACGCGTT CCAGGGGCGT GAGAAGGACT TTATCATCCT CTCCTGTGTG 
CGGGCCAATG AGCACCAAGG CATTGGATTT TTGAATGACC CCAGGCGACT CAACGTGGCC 
TTGACCAGAG CACGATACGG GGTCATTATC GTGGGCAACC CGAAGGCCCT GTCCAAGCAG 
CCGTTATGGA ACCACCTGCT GAACTACTAC AAGGAGCAGA AGGTGCTGGT GGAGGGACCC 
CTGAACAACC TGCGGGAGAG CCTCATGCAG TTCAGCAAGC CCAGGAAGCT GGTCAACACC 
ATCAACCCGG GAGCCCGCTT CATGACGACA GCCATGTATG ATGCCCGGGA GGCCATCATC 
CCAGGGTCGG TCTATGACCG GAGCAGCCAG GGCCGGCCCT CGAACATGTA CTTCCAGACC 
CACGACCAGA TTGGCATGAT CAGCGCCGGC CCCAGCCACG TGGCTGCCAT GAACATCCCC 
ATTCCCTTCA ACCTGGTCAT GCCGCCCATG CCGCCACCGG GGTATTTTGG ACAAGCTAAT 
GGGCCAGCCG CAGGCCGAGG CACCCCGAAA GGCAAGACTG GTCGTGGGGG GCGCCAGAAG 
AACCGCTTTG GGCTTCCTGG GCCCAGTCAG ACGAATCTCC CCAACAGCCA GGCCAGCCAG 
GATGTGGCGT CCCAGCCCTT CTCCCAGGGC GCCCTGACCC AAGGCTACAT CTCCATGAGC 
CAGCCCTCCC AGATGAGCCA GCCCGGCCTC TCCCAGCCTG AGCTGTCCCA GGACAGCTAC 
CTTGGAGATG AGTTTAAGTC ACAGATCGAC GTGGCGCTGT CGCAAGACTC CACATACCAG 
GGCGAGCGGG CGTACCAGCA CGGTGGAGTG ACGGGGCTGT CCCAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UPF1 ( XM_015463938.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319424.1 Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa20815
Clone ID Related Accession (Same CDS sequence)	XM_002688526.5 , XM_015463938.1
Accession Version	XM_015463938.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	regulator of nonsense transcripts 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645001.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GACATGCGGC TCATGCAAGG GGATGAGATA 
TGCCTGCGGT ACAAAGGGGA CCTGGCGCCC CTCTGGAAAG GGATCGGCCA CGTCATCAAG 
GTCCCTGATA ATTATGGCGA TGAGATTGCT ATTGAGCTTC GGAGCAGCGT GGGCGCCCCT 
GTGGAGGTGA CTCATAACTT CCAGGTGGAT TTCGTGTGGA AGTCAACCTC ATTCGACAGA 
ATGCAGAGCG CACTGAAAAC CTTTGCCGTG GATGAGACCT CCGTGTCGGG CTACATCTAC 
CACAAACTGC TTGGCCATGA GGTGGAGGAT GTGATCATCA AGTGCCAGCT GCCCAAGCGC 
TTCACGGCAC AGGGGCTCCC CGACCTCAAC CACTCGCAGG TTTACGCCGT GAAGACTGTG 
CTGCAGAGAC CACTGAGTCT GATCCAGGGC CCACCGGGCA CAGGAAAGAC CGTGACCTCA 
GCCACCATCG TCTACCACCT GGCTCGGCAA GGCAACGGGC CTGTGCTGGT CTGTGCTCCA 
AGCAACATAG CCGTGGATCA GCTGACTGAG AAGATCCACC AGACCGGGCT GAAAGTCGTG 
CGGCTCTGCG CCAAGAGCCG CGAGGCCATA GACTCCCCGG TGTCCTTCTT GGCCCTGCAC 
AACCAGATCA GGAACATGGA CAGCATGCCA GAGCTCCAAA AGCTGCAGCA GCTGAAGGAT 
GAGACTGGGG AGCTGTCGTC GGCGGACGAG AAGCGTTACA GAGCCCTGAA GCGCACTGCC 
GAGAGGGAGC TGCTCATGAA CGCAGATGTC ATCTGCTGCA CGTGTGTGGG CGCAGGAGAC 
CCGAGGCTCG CCAAGATGCA GTTCCGCTCC ATTTTAATCG ACGAAAGCAC GCAGGCCACC 
GAGCCAGAGT GCATGGTCCC TGTGGTCCTT GGAGCCAAGC AGCTGATCCT CGTGGGTGAC 
CACTGCCAGC TGGGCCCTGT GGTGATGTGC AAGAAGGCGG CCAAGGCCGG GCTGTCGCAG 
TCGCTCTTTG AACGCCTGGT GGTGCTGGGC ATCCGCCCCA TCCGCCTCCA GGTCCAGTAC 
CGGATGCACC CCGCGCTCAG CGCCTTCCCG TCCAACATCT TCTACGAGGG CTCTCTCCAG 
AATGGCGTTA CCGCAGCGGA TCGTGTAAAG AAGGGGTTTG ACTTTCAGTG GCCCCAACCG 
GATAAACCAA TGTTCTTCTA TGTGACCCAG GGCCAAGAGG AGATTGCCAG CTCAGGCACC 
TCCTACCTGA ACAGGACGGA GGCTGCAAAC GTGGAGAAGA TCACCACGAA GCTGTTGAAG 
GCGGGCGCCA AGCCGGACCA GATCGGCATC ATCACGCCCT ACGAGGGCCA GCGCTCCTAC 
CTGGTGCAGT ACATGCAGTT CAGCGGCTCC CTGCACACCA AGCTCTACCA GGAGGTGGAG 
ATTGCCAGCG TGGACGCGTT CCAGGGGCGT GAGAAGGACT TTATCATCCT CTCCTGTGTG 
CGGGCCAATG AGCACCAAGG CATTGGATTT TTGAATGACC CCAGGCGACT CAACGTGGCC 
TTGACCAGAG CACGATACGG GGTCATTATC GTGGGCAACC CGAAGGCCCT GTCCAAGCAG 
CCGTTATGGA ACCACCTGCT GAACTACTAC AAGGAGCAGA AGGTGCTGGT GGAGGGACCC 
CTGAACAACC TGCGGGAGAG CCTCATGCAG TTCAGCAAGC CCAGGAAGCT GGTCAACACC 
ATCAACCCGG GAGCCCGCTT CATGACGACA GCCATGTATG ATGCCCGGGA GGCCATCATC 
CCAGGGTCGG TCTATGACCG GAGCAGCCAG GGCCGGCCCT CGAACATGTA CTTCCAGACC 
CACGACCAGA TTGGCATGAT CAGCGCCGGC CCCAGCCACG TGGCTGCCAT GAACATCCCC 
ATTCCCTTCA ACCTGGTCAT GCCGCCCATG CCGCCACCGG GGTATTTTGG ACAAGCTAAT 
GGGCCAGCCG CAGGCCGAGG CACCCCGAAA GGCAAGACTG GTCGTGGGGG GCGCCAGAAG 
AACCGCTTTG GGCTTCCTGG GCCCAGTCAG ACGAATCTCC CCAACAGCCA GGCCAGCCAG 
GATGTGGCGT CCCAGCCCTT CTCCCAGGGC GCCCTGACCC AAGGCTACAT CTCCATGAGC 
CAGCCCTCCC AGATGAGCCA GCCCGGCCTC TCCCAGCCTG AGCTGTCCCA GGACAGCTAC 
CTTGGAGATG AGTTTAAGTC ACAGATCGAC GTGGCGCTGT CGCAAGACTC CACATACCAG 
GGCGAGCGGG CGTACCAGCA CGGTGGAGTG ACGGGGCTGT CCCAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319424.1
CDS	1..3351
Translation	MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGGGAGAPVGAGPGAAAGQLDAQVGGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPVHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSNMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY

Target ORF information:

RefSeq Version	XM_015463938.1
Organism	Bos taurus(cattle)
Definition	Bos taurus UPF1 regulator of nonsense transcripts homolog (yeast) (UPF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463938.1

ORF Insert Sequence:

ATGAGCGTGG AGGCGTACGG GCCCAGCTCG CAGACGCTCA CCTTCTTGGA CACTGAGGAG 
GCCGAGCTGC TCGGCGCCGA CACCCAGGGC TCCGAGTTCG AGTTTACCGA CTTCACCCTC 
CCCAGCCAGA CGCAGACGCC CCCCGGCGGC CCCGGAGGTG GCGGCGCGGG GGCCCCGGTC 
GGAGCCGGCC CGGGCGCGGC GGCCGGACAG CTCGACGCGC AGGTTGGTGG CCCTGAGGGC 
ATCCTGCAGA ACGGGGCCGT GGACGACAGC GTGGCCAAGA CCAGCCAGTT GTTGGCCGAA 
TTGAACTTTG AAGAAGATGA AGAAGATACC TATTACACAA AGGATCTGCC TGTACATGCC 
TGCAGTTACT GTGGAATACA CGATCCTGCT TGCGTGGTTT ACTGTAACAC CAGCAAGAAG 
TGGTTCTGTA ATGGACGTGG AAATACTTCT GGCAGCCACA TTGTAAATCA CCTTGTGAGG 
GCAAAATGCA AAGAGGTGAC TCTGCACAAG GATGGGCCCC TAGGGGAGAC GGTTCTGGAG 
TGTTATAACT GCGGCTGCCG CAACGTCTTC CTCCTCGGCT TCATCCCTGC CAAGGCCGAC 
TCGGTTGTGG TGCTGCTGTG CAGGCAGCCC TGTGCCAGTC AGAGCAGCCT CAAGGACATC 
AACTGGGACA GCTCCCAGTG GCAGCCCCTG ATCCAGGACC GGTGCTTCCT GTCCTGGCTG 
GTCAAGATCC CTTCTGAGCA GGAGCAGCTG CGGGCGCGGC AGATCACCGC CCAGCAGATC 
AACAAGCTGG AGGAGCTCTG GAAGGAGAAC CCTTCTGCCA CCTTGGAGGA CCTGGAGAAG 
CCTGGAGTGG ACGAGGAGCC GCAGCACGTC CTCCTGCGCT ACGAGGACGC CTACCAGTAC 
CAGAACATAT TTGGGCCTCT GGTCAAGCTG GAGGCTGACT ACGACAAGAA GCTGAAAGAG 
TCACAGACTC AAGATAACAT CACGGTCAGG TGGGACCTGG GCCTTAACAA GAAGAGAATC 
GCCTACTTCA CTTTGCCCAA GACTGACTCT GACATGCGGC TCATGCAAGG GGATGAGATA 
TGCCTGCGGT ACAAAGGGGA CCTGGCGCCC CTCTGGAAAG GGATCGGCCA CGTCATCAAG 
GTCCCTGATA ATTATGGCGA TGAGATTGCT ATTGAGCTTC GGAGCAGCGT GGGCGCCCCT 
GTGGAGGTGA CTCATAACTT CCAGGTGGAT TTCGTGTGGA AGTCAACCTC ATTCGACAGA 
ATGCAGAGCG CACTGAAAAC CTTTGCCGTG GATGAGACCT CCGTGTCGGG CTACATCTAC 
CACAAACTGC TTGGCCATGA GGTGGAGGAT GTGATCATCA AGTGCCAGCT GCCCAAGCGC 
TTCACGGCAC AGGGGCTCCC CGACCTCAAC CACTCGCAGG TTTACGCCGT GAAGACTGTG 
CTGCAGAGAC CACTGAGTCT GATCCAGGGC CCACCGGGCA CAGGAAAGAC CGTGACCTCA 
GCCACCATCG TCTACCACCT GGCTCGGCAA GGCAACGGGC CTGTGCTGGT CTGTGCTCCA 
AGCAACATAG CCGTGGATCA GCTGACTGAG AAGATCCACC AGACCGGGCT GAAAGTCGTG 
CGGCTCTGCG CCAAGAGCCG CGAGGCCATA GACTCCCCGG TGTCCTTCTT GGCCCTGCAC 
AACCAGATCA GGAACATGGA CAGCATGCCA GAGCTCCAAA AGCTGCAGCA GCTGAAGGAT 
GAGACTGGGG AGCTGTCGTC GGCGGACGAG AAGCGTTACA GAGCCCTGAA GCGCACTGCC 
GAGAGGGAGC TGCTCATGAA CGCAGATGTC ATCTGCTGCA CGTGTGTGGG CGCAGGAGAC 
CCGAGGCTCG CCAAGATGCA GTTCCGCTCC ATTTTAATCG ACGAAAGCAC GCAGGCCACC 
GAGCCAGAGT GCATGGTCCC TGTGGTCCTT GGAGCCAAGC AGCTGATCCT CGTGGGTGAC 
CACTGCCAGC TGGGCCCTGT GGTGATGTGC AAGAAGGCGG CCAAGGCCGG GCTGTCGCAG 
TCGCTCTTTG AACGCCTGGT GGTGCTGGGC ATCCGCCCCA TCCGCCTCCA GGTCCAGTAC 
CGGATGCACC CCGCGCTCAG CGCCTTCCCG TCCAACATCT TCTACGAGGG CTCTCTCCAG 
AATGGCGTTA CCGCAGCGGA TCGTGTAAAG AAGGGGTTTG ACTTTCAGTG GCCCCAACCG 
GATAAACCAA TGTTCTTCTA TGTGACCCAG GGCCAAGAGG AGATTGCCAG CTCAGGCACC 
TCCTACCTGA ACAGGACGGA GGCTGCAAAC GTGGAGAAGA TCACCACGAA GCTGTTGAAG 
GCGGGCGCCA AGCCGGACCA GATCGGCATC ATCACGCCCT ACGAGGGCCA GCGCTCCTAC 
CTGGTGCAGT ACATGCAGTT CAGCGGCTCC CTGCACACCA AGCTCTACCA GGAGGTGGAG 
ATTGCCAGCG TGGACGCGTT CCAGGGGCGT GAGAAGGACT TTATCATCCT CTCCTGTGTG 
CGGGCCAATG AGCACCAAGG CATTGGATTT TTGAATGACC CCAGGCGACT CAACGTGGCC 
TTGACCAGAG CACGATACGG GGTCATTATC GTGGGCAACC CGAAGGCCCT GTCCAAGCAG 
CCGTTATGGA ACCACCTGCT GAACTACTAC AAGGAGCAGA AGGTGCTGGT GGAGGGACCC 
CTGAACAACC TGCGGGAGAG CCTCATGCAG TTCAGCAAGC CCAGGAAGCT GGTCAACACC 
ATCAACCCGG GAGCCCGCTT CATGACGACA GCCATGTATG ATGCCCGGGA GGCCATCATC 
CCAGGGTCGG TCTATGACCG GAGCAGCCAG GGCCGGCCCT CGAACATGTA CTTCCAGACC 
CACGACCAGA TTGGCATGAT CAGCGCCGGC CCCAGCCACG TGGCTGCCAT GAACATCCCC 
ATTCCCTTCA ACCTGGTCAT GCCGCCCATG CCGCCACCGG GGTATTTTGG ACAAGCTAAT 
GGGCCAGCCG CAGGCCGAGG CACCCCGAAA GGCAAGACTG GTCGTGGGGG GCGCCAGAAG 
AACCGCTTTG GGCTTCCTGG GCCCAGTCAG ACGAATCTCC CCAACAGCCA GGCCAGCCAG 
GATGTGGCGT CCCAGCCCTT CTCCCAGGGC GCCCTGACCC AAGGCTACAT CTCCATGAGC 
CAGCCCTCCC AGATGAGCCA GCCCGGCCTC TCCCAGCCTG AGCTGTCCCA GGACAGCTAC 
CTTGGAGATG AGTTTAAGTC ACAGATCGAC GTGGCGCTGT CGCAAGACTC CACATACCAG 
GGCGAGCGGG CGTACCAGCA CGGTGGAGTG ACGGGGCTGT CCCAGTACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	UPF1
Entrez Gene ID	532933
Full Name	UPF1, RNA helicase and ATPase
General protein information	Preferred Names UPF1, RNA helicase and ATPase Names regulator of nonsense transcripts 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Pan troglodytes (chimpanzee)	UPF1	XP_512972.4
Mus musculus (house mouse)	Upf1	NP_001116301.1
Eremothecium gossypii	AGOS_ABR022C	NP_982968.4
	LOC720288	XP_002808229.1
Canis lupus familiaris (dog)	UPF1	XP_005632737.1
Bos taurus (cattle)	UPF1	XP_005208537.1
Saccharomyces cerevisiae (baker's yeast)	NAM7	NP_013797.1
Schizosaccharomyces pombe (fission yeast)	upf1	NP_593080.1
Magnaporthe oryzae (rice blast fungus)	MGG_00976	XP_003717973.1
Gallus gallus (chicken)	UPF1	XP_418237.3
Danio rerio (zebrafish)	upf1	NP_998639.1
Drosophila melanogaster (fruit fly)	Upf1	NP_572767.1
Oryza sativa (rice)	Os07g0495900	NP_001059698.1
Homo sapiens (human)	UPF1	NP_002902.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001133	XP_322028.4
Caenorhabditis elegans (roundworm)	smg-2	NP_490829.1
Kluyveromyces lactis	KLLA0B06435g	XP_451821.1
Neurospora crassa	NCU04242	XP_961233.1
Rattus norvegicus (Norway rat)	Upf1	XP_003751614.1
Arabidopsis thaliana (thale cress)	LBA1	NP_199512.2
Xenopus tropicalis (tropical clawed frog)	upf1	XP_002938872.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

UPF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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