KIF1A gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015462895.1	XP_015318381.1	kinesin-like protein KIF1A isoform X14
XM_015462883.1	XP_015318369.1	kinesin-like protein KIF1A isoform X1
XM_015462887.1	XP_015318373.1	kinesin-like protein KIF1A isoform X5
XM_015462890.1	XP_015318376.1	kinesin-like protein KIF1A isoform X8
XM_005205058.3	XP_005205115.1	kinesin-like protein KIF1A isoform X7
XM_010803923.3	XP_010802225.2	kinesin-like protein KIF1A isoform X1
XM_005205055.4	XP_005205112.2	kinesin-like protein KIF1A isoform X6
XM_002686593.6	XP_002686639.3	kinesin-like protein KIF1A isoform X8
XM_010803929.3	XP_010802231.1	kinesin-like protein KIF1A isoform X9
XM_005205061.4	XP_005205118.1	kinesin-like protein KIF1A isoform X14
XM_010803923.2	XP_010802225.1	kinesin-like protein KIF1A isoform X1
XM_010803925.2	XP_010802227.1	kinesin-like protein KIF1A isoform X3
XM_005205055.3	XP_005205112.1	kinesin-like protein KIF1A isoform X6
XM_015462886.1	XP_015318372.1	kinesin-like protein KIF1A isoform X4
XM_015462891.1	XP_015318377.1	kinesin-like protein KIF1A isoform X9
XM_015462896.1	XP_015318382.1	kinesin-like protein KIF1A isoform X13
XM_010803926.3	XP_010802228.2	kinesin-like protein KIF1A isoform X4
XM_024990340.1	XP_024846108.1	kinesin-like protein KIF1A isoform X11
XM_005205061.3	XP_005205118.1	kinesin-like protein KIF1A isoform X11
XM_005205062.3	XP_005205119.1	kinesin-like protein KIF1A isoform X12
XM_015462884.1	XP_015318370.1	kinesin-like protein KIF1A isoform X2
XM_015462889.1	XP_015318375.1	kinesin-like protein KIF1A isoform X7
XM_015462894.1	XP_015318380.1	kinesin-like protein KIF1A isoform X12
XM_010803924.3	XP_010802226.2	kinesin-like protein KIF1A isoform X2
XM_005205058.4	XP_005205115.2	kinesin-like protein KIF1A isoform X7
XM_024990339.1	XP_024846107.1	kinesin-like protein KIF1A isoform X10
XM_010803931.3	XP_010802233.2	kinesin-like protein KIF1A isoform X16
XM_010803929.2	XP_010802231.1	kinesin-like protein KIF1A isoform X9
XM_010803924.2	XP_010802226.1	kinesin-like protein KIF1A isoform X2
XM_002686593.5	XP_002686639.2	kinesin-like protein KIF1A isoform X8
XM_015462888.1	XP_015318374.1	kinesin-like protein KIF1A isoform X6
XM_015462892.1	XP_015318378.1	kinesin-like protein KIF1A isoform X10
XM_010803927.3	XP_010802229.2	kinesin-like protein KIF1A isoform X5
XM_024990341.1	XP_024846109.1	kinesin-like protein KIF1A isoform X12
XM_005205062.4	XP_005205119.1	kinesin-like protein KIF1A isoform X15
XM_010803926.2	XP_010802228.1	kinesin-like protein KIF1A isoform X4
XM_010803927.2	XP_010802229.1	kinesin-like protein KIF1A isoform X5
XM_010803931.2	XP_010802233.1	kinesin-like protein KIF1A isoform X13
XM_015462885.1	XP_015318371.1	kinesin-like protein KIF1A isoform X3
XM_015462893.1	XP_015318379.1	kinesin-like protein KIF1A isoform X11
XM_005205060.3	XP_005205117.1	kinesin-like protein KIF1A isoform X10
XM_010803925.3	XP_010802227.2	kinesin-like protein KIF1A isoform X3
XM_005205060.4	XP_005205117.1	kinesin-like protein KIF1A isoform X13

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa233462	XM_010803923.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233463	XM_010803924.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233464	XM_010803925.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233465	XM_010803926.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233466	XM_010803927.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233467	XM_005205055.4 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233468	XM_005205058.4 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233469	XM_002686593.6 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233470	XM_010803929.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233471	XM_024990339.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233472	XM_024990340.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233473	XM_024990341.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233474	XM_005205060.4 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233475	XM_005205061.4 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233476	XM_005205062.4 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa233477	XM_010803931.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa18299	XM_015462892.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18299	XM_005205060.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18300	XM_005205061.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18300	XM_015462893.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18301	XM_005205062.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18301	XM_015462894.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa51460	XM_010803929.2 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa51460	XM_015462891.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa18293	XM_005205055.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18293	XM_015462888.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18295	XM_015462895.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18296	XM_005205058.3 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18296	XM_015462889.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18297	XM_002686593.5 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa18297	XM_015462890.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51455	XM_010803923.2 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51455	XM_015462883.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51456	XM_010803924.2 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51456	XM_015462884.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51457	XM_010803925.2 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51457	XM_015462885.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51458	XM_010803926.2 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51458	XM_015462886.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51459	XM_010803927.2 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51459	XM_015462887.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51461	XM_010803931.2 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa51461	XM_015462896.1 Latest version!	Bos taurus kinesin family member 1A (KIF1A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

KIF1A ( XM_010803923.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802225.2 Bos taurus kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa233462
Clone ID Related Accession (Same CDS sequence)	XM_010803923.3
Accession Version	XM_010803923.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGACCT GCAGGACGAC GTCTTTCCGG AGCACGTGCT GTGCGACGGC 
CGGGACCCGT TTTACGACCG GCCCCCCCTG TTCAGTTTAG TAGGAAGGGC CTTCGTGTAC 
CTCAGCAACC TGCTGTACCC CGTGCCGCTG GTGCACCGCG TGGCCGTCGT CAGCGAGAAG 
GGCGAGGTGA AGGGCTTCCT GCGCGTGGCC GTGCAGGCCA TCTCAGCCGA CGAGGAGGCC 
CCTGACTACG GCTCCGGCGT CCGCCAGTCG GGAACAGCCA AAATCTCCTT TGACGACCAG 
CATTTTGAGA AGTTCCAGGC CGAGTCCTGC CCCGGGGTGG GCATGTCCCG CTCAGGGACC 
TCCCAGGAGG AGCTGCGGAT TGTGGAAGGC CAGGGCCAGG CCGCAGACTC CGGGCCCTCA 
GCCGACGAGG TCAACAACAA CACCTGTTCA GCGGTGACCC CGGAAGGCCT CCTGGACAGC 
CCTGAGAAAG CCGCCCTGGA TGGGCCCCTG GACGCCGCCC TGGACCACCT CGGCCTGGGC 
AGCACCTTCA CCTTCCGCGT GACCGTCCTG CAGGCGTCCA GCATATCCGC TGAATATGCC 
GACATCTTCT GCCAGTTCAA CTTCATCCAT CGCCATGACG AGGCCTTCTC TACGGAGCCC 
CTGAAGAACA CGGGGAGGGG ACCCCCGCTC GGCTTCTACC ACGTCCAAAA CATTGCGGTG 
GAGGTGACCA GGTCCTTCAT CGAGTACATC AAGAGCCAGC CCCTCGTGTT CGAGGTCTTC 
GGGCACTACC AGCAGCACCC GTTCCCGCCC CTCTGCAAGG ACGTGCTCAG CCCCCTGAGG 
CCCTCTCGCC GCCACTTCCC ACGGGTCATG CCGCTGTCCA AGCCAGTGCC GGCCACCAAG 
CTCAGCACGC TGACGCGACC CTGCCCGGGC CCCTGCCACT GCAAGTACGA CCTGCTGGTC 
TACTTCGAGA TCTGCGAGCT GGAGGCCAAC GGCGACTACA TCCCCGCAGT GGTGGATCAC 
CGCGGCGGGA TGCCGTGCAT GGGGACCTTC CTGCTCCACC AGGGCATCCA GAGACGGATC 
ACCGTGACCC TGCTGCACGA GACGGGCAGC CACATCCGCT GGAAGGAAGT GCGGGAGCTG 
GTGGTGGGTC GCATCCGAAA CACCCCGGAA ACGGACGAGT CCCTGATTGA CCCTAATATC 
TTGTCGCTCA GCATCCTCTC TTCTGGATAC ATCTGCCCAG CCCAGGATGA CCGGCAGTTT 
CTTGATTCGG ACATGCCTAG CGTTTCGTTT GGAAGTGACA CTAGAACCTT TTACCAGTTT 
GAGGCTGCGT GGGACAGCTC CATGCACAAC TCCCTCCTGC TGAACCGGGT CACCCCTTAC 
CGAGAGAAGA TTTACATGAC CCTCTCTGCT TATATCGAGA TGGAGAACTG CACGCAGCCG 
GCCGTGGTCA CCAAAGACTT CTGCATGGTC TTCTACTCGC GCGACGCCAA GCTGCCCGCC 
TCGCGCTCCA TCCGCAACCT GTTCGGCAGT GGGAGCCTGC GGGCCTCAGA GAGCAACCGC 
GTGACGGGCG TGTACGAGCT GAGTCTGTGC CACGTGGCTG ACGCGGGCAG CCCAGGCATG 
CAGCGGCGGC GCCGGCGGGT GCTGGACACA TCCGTGGCCT ACGTCCGCGG CGAGGAGAAC 
CTGGCGGGCT GGAGGCCGCG TAGTGACAGC CTCATCCTGG ACCACCAGTG GGAGCTGGAG 
AAGCTGAGTC TCCTGCAGGA GGTGGAGAAG ACCAGGCACT ACCTGTTGCT GCGAGAGAAG 
CTGGAGGCGG CCCAGCGGCC CGGCCCCGAG ACGCTGTCCC CCGCCGCCAG TGACGGCTCC 
GAGGCCCACG GCTCCTCCAG TGCCTCCTCC CCGCTCACGG CTGAGGCCCG GCCAGCATCC 
CTGGAGGCGC CCAGCGAGCG GCAGCGGGAG CTGGCTGTCA AGTGCTTACG GCTCCTCACC 
CACTCCTTCA ACAGAGAGTA CACCCACAGC CACGTCTGCG TCAGCGCCAG CGAGAGCAAG 
CTCTCTGAGA TGTCGGTCAC CCTGCTGCGG GACCCGTCCA TGTCCCCGCT GGGGGCCGCC 
ACGCTCACTC CCTCCTCTAC CTGCCCCTCC CTGGTCGAGG GCCGGTATGG GGCCACCGAC 
CTGAGGACCC CACAGCCCTG CTCCCGGCCC GCCAGCCCGG AGCCTGAGCC CCTGCCCGAG 
GTAGATGCCA AGAAGCTCCC CTCCCCTGCC CGGGCAGCGG AAGCCGACAA GGAGCCCCAG 
CGCCTGCTGG TGCCTGACAT CCAGGAAATC CGTGTCAGCC CCATCGTCTC CAAGAAGGGC 
TACCTGCACT TCCTGGAGCC GCACACGGCC GGCTGGGCCA AGCGCTTCGT GGTGGTGCGG 
CGGCCCTACG CCTACATGTA CAACCACGAC AAGGACGCCG TGGAGCGCTT CGTGCTCAAC 
CTGTCCACCG CCCAGGTGGA GTACAGCGAG GACCAGCAGG CGATGCTCAA GACCCCCAAC 
ACGTTCGCGG TGTGCACGGA GCACCGGGGC ATCTTGCTGC AGGCCAGCAG TGACAAGGAC 
ATGCACGACT GGCTGTACGC CTTCAACCCC CTGCTGGCCG GGACCATCCG GTCCAAGCTG 
TCCAGAAGGA GGTCTGCCCA GATGCGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802225.2
CDS	323..5815
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803923.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803923.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGACCT GCAGGACGAC GTCTTTCCGG AGCACGTGCT GTGCGACGGC 
CGGGACCCGT TTTACGACCG GCCCCCCCTG TTCAGTTTAG TAGGAAGGGC CTTCGTGTAC 
CTCAGCAACC TGCTGTACCC CGTGCCGCTG GTGCACCGCG TGGCCGTCGT CAGCGAGAAG 
GGCGAGGTGA AGGGCTTCCT GCGCGTGGCC GTGCAGGCCA TCTCAGCCGA CGAGGAGGCC 
CCTGACTACG GCTCCGGCGT CCGCCAGTCG GGAACAGCCA AAATCTCCTT TGACGACCAG 
CATTTTGAGA AGTTCCAGGC CGAGTCCTGC CCCGGGGTGG GCATGTCCCG CTCAGGGACC 
TCCCAGGAGG AGCTGCGGAT TGTGGAAGGC CAGGGCCAGG CCGCAGACTC CGGGCCCTCA 
GCCGACGAGG TCAACAACAA CACCTGTTCA GCGGTGACCC CGGAAGGCCT CCTGGACAGC 
CCTGAGAAAG CCGCCCTGGA TGGGCCCCTG GACGCCGCCC TGGACCACCT CGGCCTGGGC 
AGCACCTTCA CCTTCCGCGT GACCGTCCTG CAGGCGTCCA GCATATCCGC TGAATATGCC 
GACATCTTCT GCCAGTTCAA CTTCATCCAT CGCCATGACG AGGCCTTCTC TACGGAGCCC 
CTGAAGAACA CGGGGAGGGG ACCCCCGCTC GGCTTCTACC ACGTCCAAAA CATTGCGGTG 
GAGGTGACCA GGTCCTTCAT CGAGTACATC AAGAGCCAGC CCCTCGTGTT CGAGGTCTTC 
GGGCACTACC AGCAGCACCC GTTCCCGCCC CTCTGCAAGG ACGTGCTCAG CCCCCTGAGG 
CCCTCTCGCC GCCACTTCCC ACGGGTCATG CCGCTGTCCA AGCCAGTGCC GGCCACCAAG 
CTCAGCACGC TGACGCGACC CTGCCCGGGC CCCTGCCACT GCAAGTACGA CCTGCTGGTC 
TACTTCGAGA TCTGCGAGCT GGAGGCCAAC GGCGACTACA TCCCCGCAGT GGTGGATCAC 
CGCGGCGGGA TGCCGTGCAT GGGGACCTTC CTGCTCCACC AGGGCATCCA GAGACGGATC 
ACCGTGACCC TGCTGCACGA GACGGGCAGC CACATCCGCT GGAAGGAAGT GCGGGAGCTG 
GTGGTGGGTC GCATCCGAAA CACCCCGGAA ACGGACGAGT CCCTGATTGA CCCTAATATC 
TTGTCGCTCA GCATCCTCTC TTCTGGATAC ATCTGCCCAG CCCAGGATGA CCGGCAGTTT 
CTTGATTCGG ACATGCCTAG CGTTTCGTTT GGAAGTGACA CTAGAACCTT TTACCAGTTT 
GAGGCTGCGT GGGACAGCTC CATGCACAAC TCCCTCCTGC TGAACCGGGT CACCCCTTAC 
CGAGAGAAGA TTTACATGAC CCTCTCTGCT TATATCGAGA TGGAGAACTG CACGCAGCCG 
GCCGTGGTCA CCAAAGACTT CTGCATGGTC TTCTACTCGC GCGACGCCAA GCTGCCCGCC 
TCGCGCTCCA TCCGCAACCT GTTCGGCAGT GGGAGCCTGC GGGCCTCAGA GAGCAACCGC 
GTGACGGGCG TGTACGAGCT GAGTCTGTGC CACGTGGCTG ACGCGGGCAG CCCAGGCATG 
CAGCGGCGGC GCCGGCGGGT GCTGGACACA TCCGTGGCCT ACGTCCGCGG CGAGGAGAAC 
CTGGCGGGCT GGAGGCCGCG TAGTGACAGC CTCATCCTGG ACCACCAGTG GGAGCTGGAG 
AAGCTGAGTC TCCTGCAGGA GGTGGAGAAG ACCAGGCACT ACCTGTTGCT GCGAGAGAAG 
CTGGAGGCGG CCCAGCGGCC CGGCCCCGAG ACGCTGTCCC CCGCCGCCAG TGACGGCTCC 
GAGGCCCACG GCTCCTCCAG TGCCTCCTCC CCGCTCACGG CTGAGGCCCG GCCAGCATCC 
CTGGAGGCGC CCAGCGAGCG GCAGCGGGAG CTGGCTGTCA AGTGCTTACG GCTCCTCACC 
CACTCCTTCA ACAGAGAGTA CACCCACAGC CACGTCTGCG TCAGCGCCAG CGAGAGCAAG 
CTCTCTGAGA TGTCGGTCAC CCTGCTGCGG GACCCGTCCA TGTCCCCGCT GGGGGCCGCC 
ACGCTCACTC CCTCCTCTAC CTGCCCCTCC CTGGTCGAGG GCCGGTATGG GGCCACCGAC 
CTGAGGACCC CACAGCCCTG CTCCCGGCCC GCCAGCCCGG AGCCTGAGCC CCTGCCCGAG 
GTAGATGCCA AGAAGCTCCC CTCCCCTGCC CGGGCAGCGG AAGCCGACAA GGAGCCCCAG 
CGCCTGCTGG TGCCTGACAT CCAGGAAATC CGTGTCAGCC CCATCGTCTC CAAGAAGGGC 
TACCTGCACT TCCTGGAGCC GCACACGGCC GGCTGGGCCA AGCGCTTCGT GGTGGTGCGG 
CGGCCCTACG CCTACATGTA CAACCACGAC AAGGACGCCG TGGAGCGCTT CGTGCTCAAC 
CTGTCCACCG CCCAGGTGGA GTACAGCGAG GACCAGCAGG CGATGCTCAA GACCCCCAAC 
ACGTTCGCGG TGTGCACGGA GCACCGGGGC ATCTTGCTGC AGGCCAGCAG TGACAAGGAC 
ATGCACGACT GGCTGTACGC CTTCAACCCC CTGCTGGCCG GGACCATCCG GTCCAAGCTG 
TCCAGAAGGA GGTCTGCCCA GATGCGGGTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803924.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802226.2 Bos taurus kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

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CloneID	OBa233463
Clone ID Related Accession (Same CDS sequence)	XM_010803924.3
Accession Version	XM_010803924.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGACCT GCAGGACGAC GTCTTTCCGG AGCACGTGCT GTGCGACGGC 
CGGGACCCGT TTTACGACCG GCCCCCCCTG TTCAGTTTAG TAGGAAGGGC CTTCGTGTAC 
CTCAGCAACC TGCTGTACCC CGTGCCGCTG GTGCACCGCG TGGCCGTCGT CAGCGAGAAG 
GGCGAGGTGA AGGGCTTCCT GCGCGTGGCC GTGCAGGCCA TCTCAGCCGA CGAGGAGGCC 
CCTGACTACG GCTCCGGCGT CCGCCAGTCG GGAACAGCCA AAATCTCCTT TGACGACCAG 
CATTTTGAGA AGTTCCAGGC CGAGTCCTGC CCCGGGGTGG GCATGTCCCG CTCAGGGACC 
TCCCAGGAGG AGCTGCGGAT TGTGGAAGGC CAGGGCCAGG CCGCAGACTC CGGGCCCTCA 
GCCGACGAGG TCAACAACAA CACCTGTTCA GCGGTGACCC CGGAAGGCCT CCTGGACAGC 
CCTGAGAAAG CCGCCCTGGA TGGGCCCCTG GACGCCGCCC TGGACCACCT CGGCCTGGGC 
AGCACCTTCA CCTTCCGCGT GACCGTCCTG CAGGCGTCCA GCATATCCGC TGAATATGCC 
GACATCTTCT GCCAGTTCAA CTTCATCCAT CGCCATGACG AGGCCTTCTC TACGGAGCCC 
CTGAAGAACA CGGGGAGGGG ACCCCCGCTC GGCTTCTACC ACGTCCAAAA CATTGCGGTG 
GAGGTGACCA GGTCCTTCAT CGAGTACATC AAGAGCCAGC CCCTCGTGTT CGAGGTCTTC 
GGGCACTACC AGCAGCACCC GTTCCCGCCC CTCTGCAAGG ACGTGCTCAG CCCCCTGAGG 
CCCTCTCGCC GCCACTTCCC ACGGGTCATG CCGCTGTCCA AGCCAGTGCC GGCCACCAAG 
CTCAGCACGC TGACGCGACC CTGCCCGGGC CCCTGCCACT GCAAGTACGA CCTGCTGGTC 
TACTTCGAGA TCTGCGAGCT GGAGGCCAAC GGCGACTACA TCCCCGCAGT GGTGGATCAC 
CGCGGCGGGA TGCCGTGCAT GGGGACCTTC CTGCTCCACC AGGGCATCCA GAGACGGATC 
ACCGTGACCC TGCTGCACGA GACGGGCAGC CACATCCGCT GGAAGGAAGT GCGGGAGCTG 
GTGGTGGGTC GCATCCGAAA CACCCCGGAA ACGGACGAGT CCCTGATTGA CCCTAATATC 
TTGTCGCTCA GCATCCTCTC TTCTGGATAC ATCTGCCCAG CCCAGGATGA CCGGCAGTTT 
CTTGATTCGG ACATGCCTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802226.2
CDS	280..5748
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803924.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803924.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGACCT GCAGGACGAC GTCTTTCCGG AGCACGTGCT GTGCGACGGC 
CGGGACCCGT TTTACGACCG GCCCCCCCTG TTCAGTTTAG TAGGAAGGGC CTTCGTGTAC 
CTCAGCAACC TGCTGTACCC CGTGCCGCTG GTGCACCGCG TGGCCGTCGT CAGCGAGAAG 
GGCGAGGTGA AGGGCTTCCT GCGCGTGGCC GTGCAGGCCA TCTCAGCCGA CGAGGAGGCC 
CCTGACTACG GCTCCGGCGT CCGCCAGTCG GGAACAGCCA AAATCTCCTT TGACGACCAG 
CATTTTGAGA AGTTCCAGGC CGAGTCCTGC CCCGGGGTGG GCATGTCCCG CTCAGGGACC 
TCCCAGGAGG AGCTGCGGAT TGTGGAAGGC CAGGGCCAGG CCGCAGACTC CGGGCCCTCA 
GCCGACGAGG TCAACAACAA CACCTGTTCA GCGGTGACCC CGGAAGGCCT CCTGGACAGC 
CCTGAGAAAG CCGCCCTGGA TGGGCCCCTG GACGCCGCCC TGGACCACCT CGGCCTGGGC 
AGCACCTTCA CCTTCCGCGT GACCGTCCTG CAGGCGTCCA GCATATCCGC TGAATATGCC 
GACATCTTCT GCCAGTTCAA CTTCATCCAT CGCCATGACG AGGCCTTCTC TACGGAGCCC 
CTGAAGAACA CGGGGAGGGG ACCCCCGCTC GGCTTCTACC ACGTCCAAAA CATTGCGGTG 
GAGGTGACCA GGTCCTTCAT CGAGTACATC AAGAGCCAGC CCCTCGTGTT CGAGGTCTTC 
GGGCACTACC AGCAGCACCC GTTCCCGCCC CTCTGCAAGG ACGTGCTCAG CCCCCTGAGG 
CCCTCTCGCC GCCACTTCCC ACGGGTCATG CCGCTGTCCA AGCCAGTGCC GGCCACCAAG 
CTCAGCACGC TGACGCGACC CTGCCCGGGC CCCTGCCACT GCAAGTACGA CCTGCTGGTC 
TACTTCGAGA TCTGCGAGCT GGAGGCCAAC GGCGACTACA TCCCCGCAGT GGTGGATCAC 
CGCGGCGGGA TGCCGTGCAT GGGGACCTTC CTGCTCCACC AGGGCATCCA GAGACGGATC 
ACCGTGACCC TGCTGCACGA GACGGGCAGC CACATCCGCT GGAAGGAAGT GCGGGAGCTG 
GTGGTGGGTC GCATCCGAAA CACCCCGGAA ACGGACGAGT CCCTGATTGA CCCTAATATC 
TTGTCGCTCA GCATCCTCTC TTCTGGATAC ATCTGCCCAG CCCAGGATGA CCGGCAGTTT 
CTTGATTCGG ACATGCCTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803925.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802227.2 Bos taurus kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

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CloneID	OBa233464
Clone ID Related Accession (Same CDS sequence)	XM_010803925.3
Accession Version	XM_010803925.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803925.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGACCT GCAGGACGAC GTCTTTCCGG AGCACGTGCT GTGCGACGGC 
CGGGACCCGT TTTACGACCG GCCCCCCCTG TTCAGTTTAG TAGGAAGGGC CTTCGTGTAC 
CTCAGCAACC TGCTGTACCC CGTGCCGCTG GTGCACCGCG TGGCCGTCGT CAGCGAGAAG 
GGCGAGGTGA AGGGCTTCCT GCGCGTGGCC GTGCAGGCCA TCTCAGCCGA CGAGGAGGCC 
CCTGACTACG GCTCCGGCGT CCGCCAGTCG GGAACAGCCA AAATCTCCTT TGACGACCAG 
CATTTTGAGA AGTTCCAGGC CGAGTCCTGC CCCGGGGTGG GCATGTCCCG CTCAGGGACC 
TCCCAGGAGG AGCTGCGGAT TGTGGAAGGC CAGGGCCAGG CCGCAGACTC CGGGCCCTCA 
GCCGACGAGG TCAACAACAA CACCTGTTCA GCGGTGACCC CGGAAGGCCT CCTGGACAGC 
CCTGAGAAAG CCGCCCTGGA TGGGCCCCTG GACGCCGCCC TGGACCACCT CGGCCTGGGC 
AGCACCTTCA CCTTCCGCGT GACCGTCCTG CAGGCGTCCA GCATATCCGC TGAATATGCC 
GACATCTTCT GCCAGTTCAA CTTCATCCAT CGCCATGACG AGGCCTTCTC TACGGAGCCC 
CTGAAGAACA CGGGGAGGGG ACCCCCGCTC GGCTTCTACC ACGTCCAAAA CATTGCGGTG 
GAGGTGACCA GGTCCTTCAT CGAGTACATC AAGAGCCAGC CCCTCGTGTT CGAGGTCTTC 
GGGCACTACC AGCAGCACCC GTTCCCGCCC CTCTGCAAGG ACGTGCTCAG CCCCCTGAGG 
CCCTCTCGCC GCCACTTCCC ACGGGTCATG CCGCTGTCCA AGCCAGTGCC GGCCACCAAG 
CTCAGCACGC TGACGCGACC CTGCCCGGGC CCCTGCCACT GCAAGTACGA CCTGCTGGTC 
TACTTCGAGA TCTGCGAGCT GGAGGCCAAC GGCGACTACA TCCCCGCAGT GGTGGATCAC 
CGCGGCGGGA TGCCGTGCAT GGGGACCTTC CTGCTCCACC AGGGCATCCA GAGACGGATC 
ACCGTGACCC TGCTGCACGA GACGGGCAGC CACATCCGCT GGAAGGAAGT GCGGGAGCTG 
GTGGTGGGTC GCATCCGAAA CACCCCGGAA ACGGACGAGT CCCTGATTGA CCCTAATATC 
TTGTCGCTCA GCATCCTCTC TTCTGGATAC ATCTGCCCAG CCCAGGATGA CCGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802227.2
CDS	280..5745
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803925.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803925.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGACCT GCAGGACGAC GTCTTTCCGG AGCACGTGCT GTGCGACGGC 
CGGGACCCGT TTTACGACCG GCCCCCCCTG TTCAGTTTAG TAGGAAGGGC CTTCGTGTAC 
CTCAGCAACC TGCTGTACCC CGTGCCGCTG GTGCACCGCG TGGCCGTCGT CAGCGAGAAG 
GGCGAGGTGA AGGGCTTCCT GCGCGTGGCC GTGCAGGCCA TCTCAGCCGA CGAGGAGGCC 
CCTGACTACG GCTCCGGCGT CCGCCAGTCG GGAACAGCCA AAATCTCCTT TGACGACCAG 
CATTTTGAGA AGTTCCAGGC CGAGTCCTGC CCCGGGGTGG GCATGTCCCG CTCAGGGACC 
TCCCAGGAGG AGCTGCGGAT TGTGGAAGGC CAGGGCCAGG CCGCAGACTC CGGGCCCTCA 
GCCGACGAGG TCAACAACAA CACCTGTTCA GCGGTGACCC CGGAAGGCCT CCTGGACAGC 
CCTGAGAAAG CCGCCCTGGA TGGGCCCCTG GACGCCGCCC TGGACCACCT CGGCCTGGGC 
AGCACCTTCA CCTTCCGCGT GACCGTCCTG CAGGCGTCCA GCATATCCGC TGAATATGCC 
GACATCTTCT GCCAGTTCAA CTTCATCCAT CGCCATGACG AGGCCTTCTC TACGGAGCCC 
CTGAAGAACA CGGGGAGGGG ACCCCCGCTC GGCTTCTACC ACGTCCAAAA CATTGCGGTG 
GAGGTGACCA GGTCCTTCAT CGAGTACATC AAGAGCCAGC CCCTCGTGTT CGAGGTCTTC 
GGGCACTACC AGCAGCACCC GTTCCCGCCC CTCTGCAAGG ACGTGCTCAG CCCCCTGAGG 
CCCTCTCGCC GCCACTTCCC ACGGGTCATG CCGCTGTCCA AGCCAGTGCC GGCCACCAAG 
CTCAGCACGC TGACGCGACC CTGCCCGGGC CCCTGCCACT GCAAGTACGA CCTGCTGGTC 
TACTTCGAGA TCTGCGAGCT GGAGGCCAAC GGCGACTACA TCCCCGCAGT GGTGGATCAC 
CGCGGCGGGA TGCCGTGCAT GGGGACCTTC CTGCTCCACC AGGGCATCCA GAGACGGATC 
ACCGTGACCC TGCTGCACGA GACGGGCAGC CACATCCGCT GGAAGGAAGT GCGGGAGCTG 
GTGGTGGGTC GCATCCGAAA CACCCCGGAA ACGGACGAGT CCCTGATTGA CCCTAATATC 
TTGTCGCTCA GCATCCTCTC TTCTGGATAC ATCTGCCCAG CCCAGGATGA CCGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803926.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802228.2 Bos taurus kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

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CloneID	OBa233465
Clone ID Related Accession (Same CDS sequence)	XM_010803926.3
Accession Version	XM_010803926.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5466bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803926.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA ACGTGTCCGA CCTTGAGAAC 
AATAACCGTA GCCGTGGCGG GGTGGAGCTG AACCCAGCCC CTGACAATCT CTCCACAGTG 
ACCAGCGCCC TGGTGGGCAT GAGCCCCTCG TCCTCACTCT CGGCCCTGTC CAGCCGTGCC 
GCCTCCGTGT CCAGCCTGCA CGAGCGCATC TTGTTTGCCC CGGGCAGCGA GGAGGCCATC 
GAAAGGCTGA AGGAGACAGA AAAGATCATC GCTGAGCTCA ACGAGACCTG GGAGGAGAAG 
CTGCGGCGGA CGGAAGCCAT CCGGATGGAG AGGGAAGCAC TGCTGGCCGA GATGGGCGTG 
GCCATGCGGG AGGACGGTGG CACGCTGGGG GTGTTCTCTC CCAAAAAGAC GCCACATCTG 
GTCAACCTGA ATGAGGACCC GCTCATGTCC GAGTGCCTGC TCTACTACAT CAAGGATGGG 
ATCACCAGGG TGGGCCGGGA GGACGCGGAG AAGCGGCAGG ACATTGTTCT GAGCGGGCAC 
TTCATCAAGG AGGAGCACTG TGTCTTCCGG AGCGACTCAT GTGGGGGAAG TGAAGCTGTG 
GTGACCCTAG AGCCCTGTGA AGGGGCGGAC ACCTATGTCA ATGGCAAGAA AGTCACGGAG 
CCCAGCGTCC TGCGGTCAGG AAACCGCATC ATCATGGGCA AGAGCCACGT GTTCCGGTTC 
AACCACCCTG AGCAGGCGCG TCAGGAGCGC GAGCGCACGC CCTGCGCAGA GACGCCGGCC 
GAGCCCGTGG ACTGGGCCTT CGCCCAGCGC GAGCTGCTGG AGAAGCAGGG CATTGACATG 
AAGCAGGAGA TGGAGCAGAG GCTCCAGGAG CTGGAGGACC AGTACCGCCG GGAGCGCGAG 
GAGGCCACCT ACCTGCTGGA GCAGCAGCGG CTGGACTACG AGAGCAAACT GGAGGCCCTG 
CAGAAGCAGA TGGACTCCAG GTACTATCCC GAGGTGAACG AGGAGGAGGA GGAGCCGGAG 
GACGAAGTCC AGTGGACCGA GCGAGAGTGC GAGCTGGCAC TGTGGGCCTT CCGGAAGTGG 
AAGTGGTACC AGTTCACATC ATTGCGGGAC CTGCTGTGGG GCAACGCCAT CTTCCTCAAG 
GAGGCCAATG CCATCAGCGT GGAGCTCAAG AAGAAGGTCC AGTTCCAGTT TGTCCTCCTC 
ACGGACACAC TCTACTCCCC GCTGCCACCT GACCTGCTGC CCCCGGAGGC CGCCAAAGAC 
CGAGAGACTC GCCCCTTCCC GCGCACCATC GTGGCCGTGG AGGTCCAAGA CCAGAAGAAC 
GGGGCCACCC ACTACTGGAC GCTGGAGAAA CTCAGGCAGC GGCTAGACCT GATGCGGGAG 
ATGTATGACC GGGCGGCCGA GGTGCCCTCC AGCATCATCG AGGACTGTGA CAACGTGGTG 
ACCGGAGGAG ACCCCTTCTA CGACCGCTTC CCCTGGTTCC GGCTGGTGGG CAGTTCAGTC 
GTCTCTGGCT GCAACAGCTA TCCTCTTCTC AACACATGCA TGAGCGAGCG CATGGCTGCT 
CTCACCCCCT CCCCCACCTT CTCGAGCCCC GACTCCGACG CCACCGAGCC TGCCGAGGAG 
CAGAGCGTGG GGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC 
GACGTCTTTC CGGAGCACGT GCTGTGCGAC GGCCGGGACC CGTTTTACGA CCGGCCCCCC 
CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGGCAG TTTCTTGATT CGGACATGCC TAGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802228.2
CDS	280..5745
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDNVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803926.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803926.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA ACGTGTCCGA CCTTGAGAAC 
AATAACCGTA GCCGTGGCGG GGTGGAGCTG AACCCAGCCC CTGACAATCT CTCCACAGTG 
ACCAGCGCCC TGGTGGGCAT GAGCCCCTCG TCCTCACTCT CGGCCCTGTC CAGCCGTGCC 
GCCTCCGTGT CCAGCCTGCA CGAGCGCATC TTGTTTGCCC CGGGCAGCGA GGAGGCCATC 
GAAAGGCTGA AGGAGACAGA AAAGATCATC GCTGAGCTCA ACGAGACCTG GGAGGAGAAG 
CTGCGGCGGA CGGAAGCCAT CCGGATGGAG AGGGAAGCAC TGCTGGCCGA GATGGGCGTG 
GCCATGCGGG AGGACGGTGG CACGCTGGGG GTGTTCTCTC CCAAAAAGAC GCCACATCTG 
GTCAACCTGA ATGAGGACCC GCTCATGTCC GAGTGCCTGC TCTACTACAT CAAGGATGGG 
ATCACCAGGG TGGGCCGGGA GGACGCGGAG AAGCGGCAGG ACATTGTTCT GAGCGGGCAC 
TTCATCAAGG AGGAGCACTG TGTCTTCCGG AGCGACTCAT GTGGGGGAAG TGAAGCTGTG 
GTGACCCTAG AGCCCTGTGA AGGGGCGGAC ACCTATGTCA ATGGCAAGAA AGTCACGGAG 
CCCAGCGTCC TGCGGTCAGG AAACCGCATC ATCATGGGCA AGAGCCACGT GTTCCGGTTC 
AACCACCCTG AGCAGGCGCG TCAGGAGCGC GAGCGCACGC CCTGCGCAGA GACGCCGGCC 
GAGCCCGTGG ACTGGGCCTT CGCCCAGCGC GAGCTGCTGG AGAAGCAGGG CATTGACATG 
AAGCAGGAGA TGGAGCAGAG GCTCCAGGAG CTGGAGGACC AGTACCGCCG GGAGCGCGAG 
GAGGCCACCT ACCTGCTGGA GCAGCAGCGG CTGGACTACG AGAGCAAACT GGAGGCCCTG 
CAGAAGCAGA TGGACTCCAG GTACTATCCC GAGGTGAACG AGGAGGAGGA GGAGCCGGAG 
GACGAAGTCC AGTGGACCGA GCGAGAGTGC GAGCTGGCAC TGTGGGCCTT CCGGAAGTGG 
AAGTGGTACC AGTTCACATC ATTGCGGGAC CTGCTGTGGG GCAACGCCAT CTTCCTCAAG 
GAGGCCAATG CCATCAGCGT GGAGCTCAAG AAGAAGGTCC AGTTCCAGTT TGTCCTCCTC 
ACGGACACAC TCTACTCCCC GCTGCCACCT GACCTGCTGC CCCCGGAGGC CGCCAAAGAC 
CGAGAGACTC GCCCCTTCCC GCGCACCATC GTGGCCGTGG AGGTCCAAGA CCAGAAGAAC 
GGGGCCACCC ACTACTGGAC GCTGGAGAAA CTCAGGCAGC GGCTAGACCT GATGCGGGAG 
ATGTATGACC GGGCGGCCGA GGTGCCCTCC AGCATCATCG AGGACTGTGA CAACGTGGTG 
ACCGGAGGAG ACCCCTTCTA CGACCGCTTC CCCTGGTTCC GGCTGGTGGG CAGTTCAGTC 
GTCTCTGGCT GCAACAGCTA TCCTCTTCTC AACACATGCA TGAGCGAGCG CATGGCTGCT 
CTCACCCCCT CCCCCACCTT CTCGAGCCCC GACTCCGACG CCACCGAGCC TGCCGAGGAG 
CAGAGCGTGG GGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC 
GACGTCTTTC CGGAGCACGT GCTGTGCGAC GGCCGGGACC CGTTTTACGA CCGGCCCCCC 
CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGGCAG TTTCTTGATT CGGACATGCC TAGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803927.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802229.2 Bos taurus kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

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CloneID	OBa233466
Clone ID Related Accession (Same CDS sequence)	XM_010803927.3
Accession Version	XM_010803927.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803927.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGACCT GCAGGACGAC GTCTTTCCGG AGCACGTGCT GTGCGACGGC 
CGGGACCCGT TTTACGACCG GCCCCCCCTG TTCAGTTTAG TAGGAAGGGC CTTCGTGTAC 
CTCAGCAACC TGCTGTACCC CGTGCCGCTG GTGCACCGCG TGGCCGTCGT CAGCGAGAAG 
GGCGAGGTGA AGGGCTTCCT GCGCGTGGCC GTGCAGGCCA TCTCAGCCGA CGAGGAGGCC 
CCTGACTACG GCTCCGGCGT CCGCCAGTCG GGAACAGCCA AAATCTCCTT TGACGACCAG 
CATTTTGAGA AGTTCCAGGC CGAGTCCTGC CCCGGGGTGG GCATGTCCCG CTCAGGGACC 
TCCCAGGAGG AGCTGCGGAT TGTGGAAGGC CAGGGCCAGG CCGCAGACTC CGGGCCCTCA 
GCCGACGAGG TCAACAACAA CACCTGTTCA GCGGTGACCC CGGAAGGCCT CCTGGACAGC 
CCTGAGAAAG CCGCCCTGGA TGGGCCCCTG GACGCCGCCC TGGACCACCT CGGCCTGGGC 
AGCACCTTCA CCTTCCGCGT GACCGTCCTG CAGGCGTCCA GCATATCCGC TGAATATGCC 
GACATCTTCT GCCAGTTCAA CTTCATCCAT CGCCATGACG AGGCCTTCTC TACGGAGCCC 
CTGAAGAACA CGGGGAGGGG ACCCCCGCTC GGCTTCTACC ACGTCCAAAA CATTGCGGTG 
GAGGTGACCA GGTCCTTCAT CGAGTACATC AAGAGCCAGC CCCTCGTGTT CGAGGTCTTC 
GGGCACTACC AGCAGCACCC GTTCCCGCCC CTCTGCAAGG ACGTGCTCAG CCCCCTGAGG 
CCCTCTCGCC GCCACTTCCC ACGGGTCATG CCGCTGTCCA AGCCAGTGCC GGCCACCAAG 
CTCAGCACGC TGACGCGACC CTGCCCGGGC CCCTGCCACT GCAAGTACGA CCTGCTGGTC 
TACTTCGAGA TCTGCGAGCT GGAGGCCAAC GGCGACTACA TCCCCGCAGT GGTGGATCAC 
CGCGGCGGGA TGCCGTGCAT GGGGACCTTC CTGCTCCACC AGGGCATCCA GAGACGGATC 
ACCGTGACCC TGCTGCACGA GACGGGCAGC CACATCCGCT GGAAGGAAGT GCGGGAGCTG 
GTGGTGGGTC GCATCCGAAA CACCCCGGAA ACGGACGAGT CCCTGATTGA CCCTAATATC 
TTGTCGCTCA GCATCCTCTC TTCTGGATAC ATCTGCCCAG CCCAGGATGA CCGAACCTTT 
TACCAGTTTG AGGCTGCGTG GGACAGCTCC ATGCACAACT CCCTCCTGCT GAACCGGGTC 
ACCCCTTACC GAGAGAAGAT TTACATGACC CTCTCTGCTT ATATCGAGAT GGAGAACTGC 
ACGCAGCCGG CCGTGGTCAC CAAAGACTTC TGCATGGTCT TCTACTCGCG CGACGCCAAG 
CTGCCCGCCT CGCGCTCCAT CCGCAACCTG TTCGGCAGTG GGAGCCTGCG GGCCTCAGAG 
AGCAACCGCG TGACGGGCGT GTACGAGCTG AGTCTGTGCC ACGTGGCTGA CGCGGGCAGC 
CCAGGCATGC AGCGGCGGCG CCGGCGGGTG CTGGACACAT CCGTGGCCTA CGTCCGCGGC 
GAGGAGAACC TGGCGGGCTG GAGGCCGCGT AGTGACAGCC TCATCCTGGA CCACCAGTGG 
GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG GTGGAGAAGA CCAGGCACTA CCTGTTGCTG 
CGAGAGAAGC TGGAGGCGGC CCAGCGGCCC GGCCCCGAGA CGCTGTCCCC CGCCGCCAGT 
GACGGCTCCG AGGCCCACGG CTCCTCCAGT GCCTCCTCCC CGCTCACGGC TGAGGCCCGG 
CCAGCATCCC TGGAGGCGCC CAGCGAGCGG CAGCGGGAGC TGGCTGTCAA GTGCTTACGG 
CTCCTCACCC ACTCCTTCAA CAGAGAGTAC ACCCACAGCC ACGTCTGCGT CAGCGCCAGC 
GAGAGCAAGC TCTCTGAGAT GTCGGTCACC CTGCTGCGGG ACCCGTCCAT GTCCCCGCTG 
GGGGCCGCCA CGCTCACTCC CTCCTCTACC TGCCCCTCCC TGGTCGAGGG CCGGTATGGG 
GCCACCGACC TGAGGACCCC ACAGCCCTGC TCCCGGCCCG CCAGCCCGGA GCCTGAGCCC 
CTGCCCGAGG TAGATGCCAA GAAGCTCCCC TCCCCTGCCC GGGCAGCGGA AGCCGACAAG 
GAGCCCCAGC GCCTGCTGGT GCCTGACATC CAGGAAATCC GTGTCAGCCC CATCGTCTCC 
AAGAAGGGCT ACCTGCACTT CCTGGAGCCG CACACGGCCG GCTGGGCCAA GCGCTTCGTG 
GTGGTGCGGC GGCCCTACGC CTACATGTAC AACCACGACA AGGACGCCGT GGAGCGCTTC 
GTGCTCAACC TGTCCACCGC CCAGGTGGAG TACAGCGAGG ACCAGCAGGC GATGCTCAAG 
ACCCCCAACA CGTTCGCGGT GTGCACGGAG CACCGGGGCA TCTTGCTGCA GGCCAGCAGT 
GACAAGGACA TGCACGACTG GCTGTACGCC TTCAACCCCC TGCTGGCCGG GACCATCCGG 
TCCAAGCTGT CCAGAAGGAG GTCTGCCCAG ATGCGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802229.2
CDS	280..5721
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803927.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803927.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGACCT GCAGGACGAC GTCTTTCCGG AGCACGTGCT GTGCGACGGC 
CGGGACCCGT TTTACGACCG GCCCCCCCTG TTCAGTTTAG TAGGAAGGGC CTTCGTGTAC 
CTCAGCAACC TGCTGTACCC CGTGCCGCTG GTGCACCGCG TGGCCGTCGT CAGCGAGAAG 
GGCGAGGTGA AGGGCTTCCT GCGCGTGGCC GTGCAGGCCA TCTCAGCCGA CGAGGAGGCC 
CCTGACTACG GCTCCGGCGT CCGCCAGTCG GGAACAGCCA AAATCTCCTT TGACGACCAG 
CATTTTGAGA AGTTCCAGGC CGAGTCCTGC CCCGGGGTGG GCATGTCCCG CTCAGGGACC 
TCCCAGGAGG AGCTGCGGAT TGTGGAAGGC CAGGGCCAGG CCGCAGACTC CGGGCCCTCA 
GCCGACGAGG TCAACAACAA CACCTGTTCA GCGGTGACCC CGGAAGGCCT CCTGGACAGC 
CCTGAGAAAG CCGCCCTGGA TGGGCCCCTG GACGCCGCCC TGGACCACCT CGGCCTGGGC 
AGCACCTTCA CCTTCCGCGT GACCGTCCTG CAGGCGTCCA GCATATCCGC TGAATATGCC 
GACATCTTCT GCCAGTTCAA CTTCATCCAT CGCCATGACG AGGCCTTCTC TACGGAGCCC 
CTGAAGAACA CGGGGAGGGG ACCCCCGCTC GGCTTCTACC ACGTCCAAAA CATTGCGGTG 
GAGGTGACCA GGTCCTTCAT CGAGTACATC AAGAGCCAGC CCCTCGTGTT CGAGGTCTTC 
GGGCACTACC AGCAGCACCC GTTCCCGCCC CTCTGCAAGG ACGTGCTCAG CCCCCTGAGG 
CCCTCTCGCC GCCACTTCCC ACGGGTCATG CCGCTGTCCA AGCCAGTGCC GGCCACCAAG 
CTCAGCACGC TGACGCGACC CTGCCCGGGC CCCTGCCACT GCAAGTACGA CCTGCTGGTC 
TACTTCGAGA TCTGCGAGCT GGAGGCCAAC GGCGACTACA TCCCCGCAGT GGTGGATCAC 
CGCGGCGGGA TGCCGTGCAT GGGGACCTTC CTGCTCCACC AGGGCATCCA GAGACGGATC 
ACCGTGACCC TGCTGCACGA GACGGGCAGC CACATCCGCT GGAAGGAAGT GCGGGAGCTG 
GTGGTGGGTC GCATCCGAAA CACCCCGGAA ACGGACGAGT CCCTGATTGA CCCTAATATC 
TTGTCGCTCA GCATCCTCTC TTCTGGATAC ATCTGCCCAG CCCAGGATGA CCGAACCTTT 
TACCAGTTTG AGGCTGCGTG GGACAGCTCC ATGCACAACT CCCTCCTGCT GAACCGGGTC 
ACCCCTTACC GAGAGAAGAT TTACATGACC CTCTCTGCTT ATATCGAGAT GGAGAACTGC 
ACGCAGCCGG CCGTGGTCAC CAAAGACTTC TGCATGGTCT TCTACTCGCG CGACGCCAAG 
CTGCCCGCCT CGCGCTCCAT CCGCAACCTG TTCGGCAGTG GGAGCCTGCG GGCCTCAGAG 
AGCAACCGCG TGACGGGCGT GTACGAGCTG AGTCTGTGCC ACGTGGCTGA CGCGGGCAGC 
CCAGGCATGC AGCGGCGGCG CCGGCGGGTG CTGGACACAT CCGTGGCCTA CGTCCGCGGC 
GAGGAGAACC TGGCGGGCTG GAGGCCGCGT AGTGACAGCC TCATCCTGGA CCACCAGTGG 
GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG GTGGAGAAGA CCAGGCACTA CCTGTTGCTG 
CGAGAGAAGC TGGAGGCGGC CCAGCGGCCC GGCCCCGAGA CGCTGTCCCC CGCCGCCAGT 
GACGGCTCCG AGGCCCACGG CTCCTCCAGT GCCTCCTCCC CGCTCACGGC TGAGGCCCGG 
CCAGCATCCC TGGAGGCGCC CAGCGAGCGG CAGCGGGAGC TGGCTGTCAA GTGCTTACGG 
CTCCTCACCC ACTCCTTCAA CAGAGAGTAC ACCCACAGCC ACGTCTGCGT CAGCGCCAGC 
GAGAGCAAGC TCTCTGAGAT GTCGGTCACC CTGCTGCGGG ACCCGTCCAT GTCCCCGCTG 
GGGGCCGCCA CGCTCACTCC CTCCTCTACC TGCCCCTCCC TGGTCGAGGG CCGGTATGGG 
GCCACCGACC TGAGGACCCC ACAGCCCTGC TCCCGGCCCG CCAGCCCGGA GCCTGAGCCC 
CTGCCCGAGG TAGATGCCAA GAAGCTCCCC TCCCCTGCCC GGGCAGCGGA AGCCGACAAG 
GAGCCCCAGC GCCTGCTGGT GCCTGACATC CAGGAAATCC GTGTCAGCCC CATCGTCTCC 
AAGAAGGGCT ACCTGCACTT CCTGGAGCCG CACACGGCCG GCTGGGCCAA GCGCTTCGTG 
GTGGTGCGGC GGCCCTACGC CTACATGTAC AACCACGACA AGGACGCCGT GGAGCGCTTC 
GTGCTCAACC TGTCCACCGC CCAGGTGGAG TACAGCGAGG ACCAGCAGGC GATGCTCAAG 
ACCCCCAACA CGTTCGCGGT GTGCACGGAG CACCGGGGCA TCTTGCTGCA GGCCAGCAGT 
GACAAGGACA TGCACGACTG GCTGTACGCC TTCAACCCCC TGCTGGCCGG GACCATCCGG 
TCCAAGCTGT CCAGAAGGAG GTCTGCCCAG ATGCGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205055.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205112.2 Bos taurus kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

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CloneID	OBa233467
Clone ID Related Accession (Same CDS sequence)	XM_005205055.4
Accession Version	XM_005205055.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205055.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAC 
CTGCAGGACG ACGTCTTTCC GGAGCACGTG CTGTGCGACG GCCGGGACCC GTTTTACGAC 
CGGCCCCCCC TGTTCAGTTT AGTAGGAAGG GCCTTCGTGT ACCTCAGCAA CCTGCTGTAC 
CCCGTGCCGC TGGTGCACCG CGTGGCCGTC GTCAGCGAGA AGGGCGAGGT GAAGGGCTTC 
CTGCGCGTGG CCGTGCAGGC CATCTCAGCC GACGAGGAGG CCCCTGACTA CGGCTCCGGC 
GTCCGCCAGT CGGGAACAGC CAAAATCTCC TTTGACGACC AGCATTTTGA GAAGTTCCAG 
GCCGAGTCCT GCCCCGGGGT GGGCATGTCC CGCTCAGGGA CCTCCCAGGA GGAGCTGCGG 
ATTGTGGAAG GCCAGGGCCA GGCCGCAGAC TCCGGGCCCT CAGCCGACGA GGTCAACAAC 
AACACCTGTT CAGCGGTGAC CCCGGAAGGC CTCCTGGACA GCCCTGAGAA AGCCGCCCTG 
GATGGGCCCC TGGACGCCGC CCTGGACCAC CTCGGCCTGG GCAGCACCTT CACCTTCCGC 
GTGACCGTCC TGCAGGCGTC CAGCATATCC GCTGAATATG CCGACATCTT CTGCCAGTTC 
AACTTCATCC ATCGCCATGA CGAGGCCTTC TCTACGGAGC CCCTGAAGAA CACGGGGAGG 
GGACCCCCGC TCGGCTTCTA CCACGTCCAA AACATTGCGG TGGAGGTGAC CAGGTCCTTC 
ATCGAGTACA TCAAGAGCCA GCCCCTCGTG TTCGAGGTCT TCGGGCACTA CCAGCAGCAC 
CCGTTCCCGC CCCTCTGCAA GGACGTGCTC AGCCCCCTGA GGCCCTCTCG CCGCCACTTC 
CCACGGGTCA TGCCGCTGTC CAAGCCAGTG CCGGCCACCA AGCTCAGCAC GCTGACGCGA 
CCCTGCCCGG GCCCCTGCCA CTGCAAGTAC GACCTGCTGG TCTACTTCGA GATCTGCGAG 
CTGGAGGCCA ACGGCGACTA CATCCCCGCA GTGGTGGATC ACCGCGGCGG GATGCCGTGC 
ATGGGGACCT TCCTGCTCCA CCAGGGCATC CAGAGACGGA TCACCGTGAC CCTGCTGCAC 
GAGACGGGCA GCCACATCCG CTGGAAGGAA GTGCGGGAGC TGGTGGTGGG TCGCATCCGA 
AACACCCCGG AAACGGACGA GTCCCTGATT GACCCTAATA TCTTGTCGCT CAGCATCCTC 
TCTTCTGGAT ACATCTGCCC AGCCCAGGAT GACCGGCAGT TTCTTGATTC GGACATGCCT 
AGCGTTTCGT TTGGAAGTGA CACTAGAACC TTTTACCAGT TTGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCCCTCCT GCTGAACCGG GTCACCCCTT ACCGAGAGAA GATTTACATG 
ACCCTCTCTG CTTATATCGA GATGGAGAAC TGCACGCAGC CGGCCGTGGT CACCAAAGAC 
TTCTGCATGG TCTTCTACTC GCGCGACGCC AAGCTGCCCG CCTCGCGCTC CATCCGCAAC 
CTGTTCGGCA GTGGGAGCCT GCGGGCCTCA GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTGAGTCTGT GCCACGTGGC TGACGCGGGC AGCCCAGGCA TGCAGCGGCG GCGCCGGCGG 
GTGCTGGACA CATCCGTGGC CTACGTCCGC GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGTAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTGGAGA AGACCAGGCA CTACCTGTTG CTGCGAGAGA AGCTGGAGGC GGCCCAGCGG 
CCCGGCCCCG AGACGCTGTC CCCCGCCGCC AGTGACGGCT CCGAGGCCCA CGGCTCCTCC 
AGTGCCTCCT CCCCGCTCAC GGCTGAGGCC CGGCCAGCAT CCCTGGAGGC GCCCAGCGAG 
CGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGGCTCCTCA CCCACTCCTT CAACAGAGAG 
TACACCCACA GCCACGTCTG CGTCAGCGCC AGCGAGAGCA AGCTCTCTGA GATGTCGGTC 
ACCCTGCTGC GGGACCCGTC CATGTCCCCG CTGGGGGCCG CCACGCTCAC TCCCTCCTCT 
ACCTGCCCCT CCCTGGTCGA GGGCCGGTAT GGGGCCACCG ACCTGAGGAC CCCACAGCCC 
TGCTCCCGGC CCGCCAGCCC GGAGCCTGAG CCCCTGCCCG AGGTAGATGC CAAGAAGCTC 
CCCTCCCCTG CCCGGGCAGC GGAAGCCGAC AAGGAGCCCC AGCGCCTGCT GGTGCCTGAC 
ATCCAGGAAA TCCGTGTCAG CCCCATCGTC TCCAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTGC GGCGGCCCTA CGCCTACATG 
TACAACCACG ACAAGGACGC CGTGGAGCGC TTCGTGCTCA ACCTGTCCAC CGCCCAGGTG 
GAGTACAGCG AGGACCAGCA GGCGATGCTC AAGACCCCCA ACACGTTCGC GGTGTGCACG 
GAGCACCGGG GCATCTTGCT GCAGGCCAGC AGTGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CCCTGCTGGC CGGGACCATC CGGTCCAAGC TGTCCAGAAG GAGGTCTGCC 
CAGATGCGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205112.2
CDS	280..5694
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205055.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205055.4

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAC 
CTGCAGGACG ACGTCTTTCC GGAGCACGTG CTGTGCGACG GCCGGGACCC GTTTTACGAC 
CGGCCCCCCC TGTTCAGTTT AGTAGGAAGG GCCTTCGTGT ACCTCAGCAA CCTGCTGTAC 
CCCGTGCCGC TGGTGCACCG CGTGGCCGTC GTCAGCGAGA AGGGCGAGGT GAAGGGCTTC 
CTGCGCGTGG CCGTGCAGGC CATCTCAGCC GACGAGGAGG CCCCTGACTA CGGCTCCGGC 
GTCCGCCAGT CGGGAACAGC CAAAATCTCC TTTGACGACC AGCATTTTGA GAAGTTCCAG 
GCCGAGTCCT GCCCCGGGGT GGGCATGTCC CGCTCAGGGA CCTCCCAGGA GGAGCTGCGG 
ATTGTGGAAG GCCAGGGCCA GGCCGCAGAC TCCGGGCCCT CAGCCGACGA GGTCAACAAC 
AACACCTGTT CAGCGGTGAC CCCGGAAGGC CTCCTGGACA GCCCTGAGAA AGCCGCCCTG 
GATGGGCCCC TGGACGCCGC CCTGGACCAC CTCGGCCTGG GCAGCACCTT CACCTTCCGC 
GTGACCGTCC TGCAGGCGTC CAGCATATCC GCTGAATATG CCGACATCTT CTGCCAGTTC 
AACTTCATCC ATCGCCATGA CGAGGCCTTC TCTACGGAGC CCCTGAAGAA CACGGGGAGG 
GGACCCCCGC TCGGCTTCTA CCACGTCCAA AACATTGCGG TGGAGGTGAC CAGGTCCTTC 
ATCGAGTACA TCAAGAGCCA GCCCCTCGTG TTCGAGGTCT TCGGGCACTA CCAGCAGCAC 
CCGTTCCCGC CCCTCTGCAA GGACGTGCTC AGCCCCCTGA GGCCCTCTCG CCGCCACTTC 
CCACGGGTCA TGCCGCTGTC CAAGCCAGTG CCGGCCACCA AGCTCAGCAC GCTGACGCGA 
CCCTGCCCGG GCCCCTGCCA CTGCAAGTAC GACCTGCTGG TCTACTTCGA GATCTGCGAG 
CTGGAGGCCA ACGGCGACTA CATCCCCGCA GTGGTGGATC ACCGCGGCGG GATGCCGTGC 
ATGGGGACCT TCCTGCTCCA CCAGGGCATC CAGAGACGGA TCACCGTGAC CCTGCTGCAC 
GAGACGGGCA GCCACATCCG CTGGAAGGAA GTGCGGGAGC TGGTGGTGGG TCGCATCCGA 
AACACCCCGG AAACGGACGA GTCCCTGATT GACCCTAATA TCTTGTCGCT CAGCATCCTC 
TCTTCTGGAT ACATCTGCCC AGCCCAGGAT GACCGGCAGT TTCTTGATTC GGACATGCCT 
AGCGTTTCGT TTGGAAGTGA CACTAGAACC TTTTACCAGT TTGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCCCTCCT GCTGAACCGG GTCACCCCTT ACCGAGAGAA GATTTACATG 
ACCCTCTCTG CTTATATCGA GATGGAGAAC TGCACGCAGC CGGCCGTGGT CACCAAAGAC 
TTCTGCATGG TCTTCTACTC GCGCGACGCC AAGCTGCCCG CCTCGCGCTC CATCCGCAAC 
CTGTTCGGCA GTGGGAGCCT GCGGGCCTCA GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTGAGTCTGT GCCACGTGGC TGACGCGGGC AGCCCAGGCA TGCAGCGGCG GCGCCGGCGG 
GTGCTGGACA CATCCGTGGC CTACGTCCGC GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGTAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTGGAGA AGACCAGGCA CTACCTGTTG CTGCGAGAGA AGCTGGAGGC GGCCCAGCGG 
CCCGGCCCCG AGACGCTGTC CCCCGCCGCC AGTGACGGCT CCGAGGCCCA CGGCTCCTCC 
AGTGCCTCCT CCCCGCTCAC GGCTGAGGCC CGGCCAGCAT CCCTGGAGGC GCCCAGCGAG 
CGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGGCTCCTCA CCCACTCCTT CAACAGAGAG 
TACACCCACA GCCACGTCTG CGTCAGCGCC AGCGAGAGCA AGCTCTCTGA GATGTCGGTC 
ACCCTGCTGC GGGACCCGTC CATGTCCCCG CTGGGGGCCG CCACGCTCAC TCCCTCCTCT 
ACCTGCCCCT CCCTGGTCGA GGGCCGGTAT GGGGCCACCG ACCTGAGGAC CCCACAGCCC 
TGCTCCCGGC CCGCCAGCCC GGAGCCTGAG CCCCTGCCCG AGGTAGATGC CAAGAAGCTC 
CCCTCCCCTG CCCGGGCAGC GGAAGCCGAC AAGGAGCCCC AGCGCCTGCT GGTGCCTGAC 
ATCCAGGAAA TCCGTGTCAG CCCCATCGTC TCCAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTGC GGCGGCCCTA CGCCTACATG 
TACAACCACG ACAAGGACGC CGTGGAGCGC TTCGTGCTCA ACCTGTCCAC CGCCCAGGTG 
GAGTACAGCG AGGACCAGCA GGCGATGCTC AAGACCCCCA ACACGTTCGC GGTGTGCACG 
GAGCACCGGG GCATCTTGCT GCAGGCCAGC AGTGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CCCTGCTGGC CGGGACCATC CGGTCCAAGC TGTCCAGAAG GAGGTCTGCC 
CAGATGCGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205058.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205115.2 Bos taurus kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

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CloneID	OBa233468
Clone ID Related Accession (Same CDS sequence)	XM_005205058.4
Accession Version	XM_005205058.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5388bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205058.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GACCTGCAGG ACGACGTCTT TCCGGAGCAC 
GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC GACCGGCCCC CCCTGTTCAG TTTAGTAGGA 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGGC AGTTTCTTGA TTCGGACATG CCTAGCGTTT CGTTTGGAAG TGACACTAGA 
ACCTTTTACC AGTTTGAGGC TGCGTGGGAC AGCTCCATGC ACAACTCCCT CCTGCTGAAC 
CGGGTCACCC CTTACCGAGA GAAGATTTAC ATGACCCTCT CTGCTTATAT CGAGATGGAG 
AACTGCACGC AGCCGGCCGT GGTCACCAAA GACTTCTGCA TGGTCTTCTA CTCGCGCGAC 
GCCAAGCTGC CCGCCTCGCG CTCCATCCGC AACCTGTTCG GCAGTGGGAG CCTGCGGGCC 
TCAGAGAGCA ACCGCGTGAC GGGCGTGTAC GAGCTGAGTC TGTGCCACGT GGCTGACGCG 
GGCAGCCCAG GCATGCAGCG GCGGCGCCGG CGGGTGCTGG ACACATCCGT GGCCTACGTC 
CGCGGCGAGG AGAACCTGGC GGGCTGGAGG CCGCGTAGTG ACAGCCTCAT CCTGGACCAC 
CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG CAGGAGGTGG AGAAGACCAG GCACTACCTG 
TTGCTGCGAG AGAAGCTGGA GGCGGCCCAG CGGCCCGGCC CCGAGACGCT GTCCCCCGCC 
GCCAGTGACG GCTCCGAGGC CCACGGCTCC TCCAGTGCCT CCTCCCCGCT CACGGCTGAG 
GCCCGGCCAG CATCCCTGGA GGCGCCCAGC GAGCGGCAGC GGGAGCTGGC TGTCAAGTGC 
TTACGGCTCC TCACCCACTC CTTCAACAGA GAGTACACCC ACAGCCACGT CTGCGTCAGC 
GCCAGCGAGA GCAAGCTCTC TGAGATGTCG GTCACCCTGC TGCGGGACCC GTCCATGTCC 
CCGCTGGGGG CCGCCACGCT CACTCCCTCC TCTACCTGCC CCTCCCTGGT CGAGGGCCGG 
TATGGGGCCA CCGACCTGAG GACCCCACAG CCCTGCTCCC GGCCCGCCAG CCCGGAGCCT 
GAGCCCCTGC CCGAGGTAGA TGCCAAGAAG CTCCCCTCCC CTGCCCGGGC AGCGGAAGCC 
GACAAGGAGC CCCAGCGCCT GCTGGTGCCT GACATCCAGG AAATCCGTGT CAGCCCCATC 
GTCTCCAAGA AGGGCTACCT GCACTTCCTG GAGCCGCACA CGGCCGGCTG GGCCAAGCGC 
TTCGTGGTGG TGCGGCGGCC CTACGCCTAC ATGTACAACC ACGACAAGGA CGCCGTGGAG 
CGCTTCGTGC TCAACCTGTC CACCGCCCAG GTGGAGTACA GCGAGGACCA GCAGGCGATG 
CTCAAGACCC CCAACACGTT CGCGGTGTGC ACGGAGCACC GGGGCATCTT GCTGCAGGCC 
AGCAGTGACA AGGACATGCA CGACTGGCTG TACGCCTTCA ACCCCCTGCT GGCCGGGACC 
ATCCGGTCCA AGCTGTCCAG AAGGAGGTCT GCCCAGATGC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205115.2
CDS	280..5667
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205058.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205058.4

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GACCTGCAGG ACGACGTCTT TCCGGAGCAC 
GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC GACCGGCCCC CCCTGTTCAG TTTAGTAGGA 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGGC AGTTTCTTGA TTCGGACATG CCTAGCGTTT CGTTTGGAAG TGACACTAGA 
ACCTTTTACC AGTTTGAGGC TGCGTGGGAC AGCTCCATGC ACAACTCCCT CCTGCTGAAC 
CGGGTCACCC CTTACCGAGA GAAGATTTAC ATGACCCTCT CTGCTTATAT CGAGATGGAG 
AACTGCACGC AGCCGGCCGT GGTCACCAAA GACTTCTGCA TGGTCTTCTA CTCGCGCGAC 
GCCAAGCTGC CCGCCTCGCG CTCCATCCGC AACCTGTTCG GCAGTGGGAG CCTGCGGGCC 
TCAGAGAGCA ACCGCGTGAC GGGCGTGTAC GAGCTGAGTC TGTGCCACGT GGCTGACGCG 
GGCAGCCCAG GCATGCAGCG GCGGCGCCGG CGGGTGCTGG ACACATCCGT GGCCTACGTC 
CGCGGCGAGG AGAACCTGGC GGGCTGGAGG CCGCGTAGTG ACAGCCTCAT CCTGGACCAC 
CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG CAGGAGGTGG AGAAGACCAG GCACTACCTG 
TTGCTGCGAG AGAAGCTGGA GGCGGCCCAG CGGCCCGGCC CCGAGACGCT GTCCCCCGCC 
GCCAGTGACG GCTCCGAGGC CCACGGCTCC TCCAGTGCCT CCTCCCCGCT CACGGCTGAG 
GCCCGGCCAG CATCCCTGGA GGCGCCCAGC GAGCGGCAGC GGGAGCTGGC TGTCAAGTGC 
TTACGGCTCC TCACCCACTC CTTCAACAGA GAGTACACCC ACAGCCACGT CTGCGTCAGC 
GCCAGCGAGA GCAAGCTCTC TGAGATGTCG GTCACCCTGC TGCGGGACCC GTCCATGTCC 
CCGCTGGGGG CCGCCACGCT CACTCCCTCC TCTACCTGCC CCTCCCTGGT CGAGGGCCGG 
TATGGGGCCA CCGACCTGAG GACCCCACAG CCCTGCTCCC GGCCCGCCAG CCCGGAGCCT 
GAGCCCCTGC CCGAGGTAGA TGCCAAGAAG CTCCCCTCCC CTGCCCGGGC AGCGGAAGCC 
GACAAGGAGC CCCAGCGCCT GCTGGTGCCT GACATCCAGG AAATCCGTGT CAGCCCCATC 
GTCTCCAAGA AGGGCTACCT GCACTTCCTG GAGCCGCACA CGGCCGGCTG GGCCAAGCGC 
TTCGTGGTGG TGCGGCGGCC CTACGCCTAC ATGTACAACC ACGACAAGGA CGCCGTGGAG 
CGCTTCGTGC TCAACCTGTC CACCGCCCAG GTGGAGTACA GCGAGGACCA GCAGGCGATG 
CTCAAGACCC CCAACACGTT CGCGGTGTGC ACGGAGCACC GGGGCATCTT GCTGCAGGCC 
AGCAGTGACA AGGACATGCA CGACTGGCTG TACGCCTTCA ACCCCCTGCT GGCCGGGACC 
ATCCGGTCCA AGCTGTCCAG AAGGAGGTCT GCCCAGATGC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_002686593.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002686639.3 Bos taurus kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

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CloneID	OBa233469
Clone ID Related Accession (Same CDS sequence)	XM_002686593.6
Accession Version	XM_002686593.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5364bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002686593.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAC 
CTGCAGGACG ACGTCTTTCC GGAGCACGTG CTGTGCGACG GCCGGGACCC GTTTTACGAC 
CGGCCCCCCC TGTTCAGTTT AGTAGGAAGG GCCTTCGTGT ACCTCAGCAA CCTGCTGTAC 
CCCGTGCCGC TGGTGCACCG CGTGGCCGTC GTCAGCGAGA AGGGCGAGGT GAAGGGCTTC 
CTGCGCGTGG CCGTGCAGGC CATCTCAGCC GACGAGGAGG CCCCTGACTA CGGCTCCGGC 
GTCCGCCAGT CGGGAACAGC CAAAATCTCC TTTGACGACC AGCATTTTGA GAAGTTCCAG 
GCCGAGTCCT GCCCCGGGGT GGGCATGTCC CGCTCAGGGA CCTCCCAGGA GGAGCTGCGG 
ATTGTGGAAG GCCAGGGCCA GGCCGCAGAC TCCGGGCCCT CAGCCGACGA GGTCAACAAC 
AACACCTGTT CAGCGGTGAC CCCGGAAGGC CTCCTGGACA GCCCTGAGAA AGCCGCCCTG 
GATGGGCCCC TGGACGCCGC CCTGGACCAC CTCGGCCTGG GCAGCACCTT CACCTTCCGC 
GTGACCGTCC TGCAGGCGTC CAGCATATCC GCTGAATATG CCGACATCTT CTGCCAGTTC 
AACTTCATCC ATCGCCATGA CGAGGCCTTC TCTACGGAGC CCCTGAAGAA CACGGGGAGG 
GGACCCCCGC TCGGCTTCTA CCACGTCCAA AACATTGCGG TGGAGGTGAC CAGGTCCTTC 
ATCGAGTACA TCAAGAGCCA GCCCCTCGTG TTCGAGGTCT TCGGGCACTA CCAGCAGCAC 
CCGTTCCCGC CCCTCTGCAA GGACGTGCTC AGCCCCCTGA GGCCCTCTCG CCGCCACTTC 
CCACGGGTCA TGCCGCTGTC CAAGCCAGTG CCGGCCACCA AGCTCAGCAC GCTGACGCGA 
CCCTGCCCGG GCCCCTGCCA CTGCAAGTAC GACCTGCTGG TCTACTTCGA GATCTGCGAG 
CTGGAGGCCA ACGGCGACTA CATCCCCGCA GTGGTGGATC ACCGCGGCGG GATGCCGTGC 
ATGGGGACCT TCCTGCTCCA CCAGGGCATC CAGAGACGGA TCACCGTGAC CCTGCTGCAC 
GAGACGGGCA GCCACATCCG CTGGAAGGAA GTGCGGGAGC TGGTGGTGGG TCGCATCCGA 
AACACCCCGG AAACGGACGA GTCCCTGATT GACCCTAATA TCTTGTCGCT CAGCATCCTC 
TCTTCTGGAT ACATCTGCCC AGCCCAGGAT GACCGAACCT TTTACCAGTT TGAGGCTGCG 
TGGGACAGCT CCATGCACAA CTCCCTCCTG CTGAACCGGG TCACCCCTTA CCGAGAGAAG 
ATTTACATGA CCCTCTCTGC TTATATCGAG ATGGAGAACT GCACGCAGCC GGCCGTGGTC 
ACCAAAGACT TCTGCATGGT CTTCTACTCG CGCGACGCCA AGCTGCCCGC CTCGCGCTCC 
ATCCGCAACC TGTTCGGCAG TGGGAGCCTG CGGGCCTCAG AGAGCAACCG CGTGACGGGC 
GTGTACGAGC TGAGTCTGTG CCACGTGGCT GACGCGGGCA GCCCAGGCAT GCAGCGGCGG 
CGCCGGCGGG TGCTGGACAC ATCCGTGGCC TACGTCCGCG GCGAGGAGAA CCTGGCGGGC 
TGGAGGCCGC GTAGTGACAG CCTCATCCTG GACCACCAGT GGGAGCTGGA GAAGCTGAGT 
CTCCTGCAGG AGGTGGAGAA GACCAGGCAC TACCTGTTGC TGCGAGAGAA GCTGGAGGCG 
GCCCAGCGGC CCGGCCCCGA GACGCTGTCC CCCGCCGCCA GTGACGGCTC CGAGGCCCAC 
GGCTCCTCCA GTGCCTCCTC CCCGCTCACG GCTGAGGCCC GGCCAGCATC CCTGGAGGCG 
CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC AAGTGCTTAC GGCTCCTCAC CCACTCCTTC 
AACAGAGAGT ACACCCACAG CCACGTCTGC GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG 
ATGTCGGTCA CCCTGCTGCG GGACCCGTCC ATGTCCCCGC TGGGGGCCGC CACGCTCACT 
CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG GGCCGGTATG GGGCCACCGA CCTGAGGACC 
CCACAGCCCT GCTCCCGGCC CGCCAGCCCG GAGCCTGAGC CCCTGCCCGA GGTAGATGCC 
AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG GAAGCCGACA AGGAGCCCCA GCGCCTGCTG 
GTGCCTGACA TCCAGGAAAT CCGTGTCAGC CCCATCGTCT CCAAGAAGGG CTACCTGCAC 
TTCCTGGAGC CGCACACGGC CGGCTGGGCC AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC 
GCCTACATGT ACAACCACGA CAAGGACGCC GTGGAGCGCT TCGTGCTCAA CCTGTCCACC 
GCCCAGGTGG AGTACAGCGA GGACCAGCAG GCGATGCTCA AGACCCCCAA CACGTTCGCG 
GTGTGCACGG AGCACCGGGG CATCTTGCTG CAGGCCAGCA GTGACAAGGA CATGCACGAC 
TGGCTGTACG CCTTCAACCC CCTGCTGGCC GGGACCATCC GGTCCAAGCT GTCCAGAAGG 
AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002686639.3
CDS	280..5643
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_002686593.6
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002686593.6

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAC 
CTGCAGGACG ACGTCTTTCC GGAGCACGTG CTGTGCGACG GCCGGGACCC GTTTTACGAC 
CGGCCCCCCC TGTTCAGTTT AGTAGGAAGG GCCTTCGTGT ACCTCAGCAA CCTGCTGTAC 
CCCGTGCCGC TGGTGCACCG CGTGGCCGTC GTCAGCGAGA AGGGCGAGGT GAAGGGCTTC 
CTGCGCGTGG CCGTGCAGGC CATCTCAGCC GACGAGGAGG CCCCTGACTA CGGCTCCGGC 
GTCCGCCAGT CGGGAACAGC CAAAATCTCC TTTGACGACC AGCATTTTGA GAAGTTCCAG 
GCCGAGTCCT GCCCCGGGGT GGGCATGTCC CGCTCAGGGA CCTCCCAGGA GGAGCTGCGG 
ATTGTGGAAG GCCAGGGCCA GGCCGCAGAC TCCGGGCCCT CAGCCGACGA GGTCAACAAC 
AACACCTGTT CAGCGGTGAC CCCGGAAGGC CTCCTGGACA GCCCTGAGAA AGCCGCCCTG 
GATGGGCCCC TGGACGCCGC CCTGGACCAC CTCGGCCTGG GCAGCACCTT CACCTTCCGC 
GTGACCGTCC TGCAGGCGTC CAGCATATCC GCTGAATATG CCGACATCTT CTGCCAGTTC 
AACTTCATCC ATCGCCATGA CGAGGCCTTC TCTACGGAGC CCCTGAAGAA CACGGGGAGG 
GGACCCCCGC TCGGCTTCTA CCACGTCCAA AACATTGCGG TGGAGGTGAC CAGGTCCTTC 
ATCGAGTACA TCAAGAGCCA GCCCCTCGTG TTCGAGGTCT TCGGGCACTA CCAGCAGCAC 
CCGTTCCCGC CCCTCTGCAA GGACGTGCTC AGCCCCCTGA GGCCCTCTCG CCGCCACTTC 
CCACGGGTCA TGCCGCTGTC CAAGCCAGTG CCGGCCACCA AGCTCAGCAC GCTGACGCGA 
CCCTGCCCGG GCCCCTGCCA CTGCAAGTAC GACCTGCTGG TCTACTTCGA GATCTGCGAG 
CTGGAGGCCA ACGGCGACTA CATCCCCGCA GTGGTGGATC ACCGCGGCGG GATGCCGTGC 
ATGGGGACCT TCCTGCTCCA CCAGGGCATC CAGAGACGGA TCACCGTGAC CCTGCTGCAC 
GAGACGGGCA GCCACATCCG CTGGAAGGAA GTGCGGGAGC TGGTGGTGGG TCGCATCCGA 
AACACCCCGG AAACGGACGA GTCCCTGATT GACCCTAATA TCTTGTCGCT CAGCATCCTC 
TCTTCTGGAT ACATCTGCCC AGCCCAGGAT GACCGAACCT TTTACCAGTT TGAGGCTGCG 
TGGGACAGCT CCATGCACAA CTCCCTCCTG CTGAACCGGG TCACCCCTTA CCGAGAGAAG 
ATTTACATGA CCCTCTCTGC TTATATCGAG ATGGAGAACT GCACGCAGCC GGCCGTGGTC 
ACCAAAGACT TCTGCATGGT CTTCTACTCG CGCGACGCCA AGCTGCCCGC CTCGCGCTCC 
ATCCGCAACC TGTTCGGCAG TGGGAGCCTG CGGGCCTCAG AGAGCAACCG CGTGACGGGC 
GTGTACGAGC TGAGTCTGTG CCACGTGGCT GACGCGGGCA GCCCAGGCAT GCAGCGGCGG 
CGCCGGCGGG TGCTGGACAC ATCCGTGGCC TACGTCCGCG GCGAGGAGAA CCTGGCGGGC 
TGGAGGCCGC GTAGTGACAG CCTCATCCTG GACCACCAGT GGGAGCTGGA GAAGCTGAGT 
CTCCTGCAGG AGGTGGAGAA GACCAGGCAC TACCTGTTGC TGCGAGAGAA GCTGGAGGCG 
GCCCAGCGGC CCGGCCCCGA GACGCTGTCC CCCGCCGCCA GTGACGGCTC CGAGGCCCAC 
GGCTCCTCCA GTGCCTCCTC CCCGCTCACG GCTGAGGCCC GGCCAGCATC CCTGGAGGCG 
CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC AAGTGCTTAC GGCTCCTCAC CCACTCCTTC 
AACAGAGAGT ACACCCACAG CCACGTCTGC GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG 
ATGTCGGTCA CCCTGCTGCG GGACCCGTCC ATGTCCCCGC TGGGGGCCGC CACGCTCACT 
CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG GGCCGGTATG GGGCCACCGA CCTGAGGACC 
CCACAGCCCT GCTCCCGGCC CGCCAGCCCG GAGCCTGAGC CCCTGCCCGA GGTAGATGCC 
AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG GAAGCCGACA AGGAGCCCCA GCGCCTGCTG 
GTGCCTGACA TCCAGGAAAT CCGTGTCAGC CCCATCGTCT CCAAGAAGGG CTACCTGCAC 
TTCCTGGAGC CGCACACGGC CGGCTGGGCC AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC 
GCCTACATGT ACAACCACGA CAAGGACGCC GTGGAGCGCT TCGTGCTCAA CCTGTCCACC 
GCCCAGGTGG AGTACAGCGA GGACCAGCAG GCGATGCTCA AGACCCCCAA CACGTTCGCG 
GTGTGCACGG AGCACCGGGG CATCTTGCTG CAGGCCAGCA GTGACAAGGA CATGCACGAC 
TGGCTGTACG CCTTCAACCC CCTGCTGGCC GGGACCATCC GGTCCAAGCT GTCCAGAAGG 
AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803929.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802231.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

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CloneID	OBa233470
Clone ID Related Accession (Same CDS sequence)	XM_010803929.3
Accession Version	XM_010803929.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803929.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGGCAG TTTCTTGATT CGGACATGCC TAGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802231.1
CDS	280..5505
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803929.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803929.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGGCAG TTTCTTGATT CGGACATGCC TAGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_024990339.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846107.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

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CloneID	OBa233471
Clone ID Related Accession (Same CDS sequence)	XM_024990339.1
Accession Version	XM_024990339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGCGTT TCGTTTGGAA GTGACACTAG AACCTTTTAC 
CAGTTTGAGG CTGCGTGGGA CAGCTCCATG CACAACTCCC TCCTGCTGAA CCGGGTCACC 
CCTTACCGAG AGAAGATTTA CATGACCCTC TCTGCTTATA TCGAGATGGA GAACTGCACG 
CAGCCGGCCG TGGTCACCAA AGACTTCTGC ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG 
CCCGCCTCGC GCTCCATCCG CAACCTGTTC GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC 
AACCGCGTGA CGGGCGTGTA CGAGCTGAGT CTGTGCCACG TGGCTGACGC GGGCAGCCCA 
GGCATGCAGC GGCGGCGCCG GCGGGTGCTG GACACATCCG TGGCCTACGT CCGCGGCGAG 
GAGAACCTGG CGGGCTGGAG GCCGCGTAGT GACAGCCTCA TCCTGGACCA CCAGTGGGAG 
CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG GAGAAGACCA GGCACTACCT GTTGCTGCGA 
GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC CCCGAGACGC TGTCCCCCGC CGCCAGTGAC 
GGCTCCGAGG CCCACGGCTC CTCCAGTGCC TCCTCCCCGC TCACGGCTGA GGCCCGGCCA 
GCATCCCTGG AGGCGCCCAG CGAGCGGCAG CGGGAGCTGG CTGTCAAGTG CTTACGGCTC 
CTCACCCACT CCTTCAACAG AGAGTACACC CACAGCCACG TCTGCGTCAG CGCCAGCGAG 
AGCAAGCTCT CTGAGATGTC GGTCACCCTG CTGCGGGACC CGTCCATGTC CCCGCTGGGG 
GCCGCCACGC TCACTCCCTC CTCTACCTGC CCCTCCCTGG TCGAGGGCCG GTATGGGGCC 
ACCGACCTGA GGACCCCACA GCCCTGCTCC CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG 
CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC CCTGCCCGGG CAGCGGAAGC CGACAAGGAG 
CCCCAGCGCC TGCTGGTGCC TGACATCCAG GAAATCCGTG TCAGCCCCAT CGTCTCCAAG 
AAGGGCTACC TGCACTTCCT GGAGCCGCAC ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG 
GTGCGGCGGC CCTACGCCTA CATGTACAAC CACGACAAGG ACGCCGTGGA GCGCTTCGTG 
CTCAACCTGT CCACCGCCCA GGTGGAGTAC AGCGAGGACC AGCAGGCGAT GCTCAAGACC 
CCCAACACGT TCGCGGTGTG CACGGAGCAC CGGGGCATCT TGCTGCAGGC CAGCAGTGAC 
AAGGACATGC ACGACTGGCT GTACGCCTTC AACCCCCTGC TGGCCGGGAC CATCCGGTCC 
AAGCTGTCCA GAAGGAGGTC TGCCCAGATG CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846107.1
CDS	280..5478
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_024990339.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990339.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGCGTT TCGTTTGGAA GTGACACTAG AACCTTTTAC 
CAGTTTGAGG CTGCGTGGGA CAGCTCCATG CACAACTCCC TCCTGCTGAA CCGGGTCACC 
CCTTACCGAG AGAAGATTTA CATGACCCTC TCTGCTTATA TCGAGATGGA GAACTGCACG 
CAGCCGGCCG TGGTCACCAA AGACTTCTGC ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG 
CCCGCCTCGC GCTCCATCCG CAACCTGTTC GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC 
AACCGCGTGA CGGGCGTGTA CGAGCTGAGT CTGTGCCACG TGGCTGACGC GGGCAGCCCA 
GGCATGCAGC GGCGGCGCCG GCGGGTGCTG GACACATCCG TGGCCTACGT CCGCGGCGAG 
GAGAACCTGG CGGGCTGGAG GCCGCGTAGT GACAGCCTCA TCCTGGACCA CCAGTGGGAG 
CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG GAGAAGACCA GGCACTACCT GTTGCTGCGA 
GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC CCCGAGACGC TGTCCCCCGC CGCCAGTGAC 
GGCTCCGAGG CCCACGGCTC CTCCAGTGCC TCCTCCCCGC TCACGGCTGA GGCCCGGCCA 
GCATCCCTGG AGGCGCCCAG CGAGCGGCAG CGGGAGCTGG CTGTCAAGTG CTTACGGCTC 
CTCACCCACT CCTTCAACAG AGAGTACACC CACAGCCACG TCTGCGTCAG CGCCAGCGAG 
AGCAAGCTCT CTGAGATGTC GGTCACCCTG CTGCGGGACC CGTCCATGTC CCCGCTGGGG 
GCCGCCACGC TCACTCCCTC CTCTACCTGC CCCTCCCTGG TCGAGGGCCG GTATGGGGCC 
ACCGACCTGA GGACCCCACA GCCCTGCTCC CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG 
CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC CCTGCCCGGG CAGCGGAAGC CGACAAGGAG 
CCCCAGCGCC TGCTGGTGCC TGACATCCAG GAAATCCGTG TCAGCCCCAT CGTCTCCAAG 
AAGGGCTACC TGCACTTCCT GGAGCCGCAC ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG 
GTGCGGCGGC CCTACGCCTA CATGTACAAC CACGACAAGG ACGCCGTGGA GCGCTTCGTG 
CTCAACCTGT CCACCGCCCA GGTGGAGTAC AGCGAGGACC AGCAGGCGAT GCTCAAGACC 
CCCAACACGT TCGCGGTGTG CACGGAGCAC CGGGGCATCT TGCTGCAGGC CAGCAGTGAC 
AAGGACATGC ACGACTGGCT GTACGCCTTC AACCCCCTGC TGGCCGGGAC CATCCGGTCC 
AAGCTGTCCA GAAGGAGGTC TGCCCAGATG CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_024990340.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846108.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

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CloneID	OBa233472
Clone ID Related Accession (Same CDS sequence)	XM_024990340.1
Accession Version	XM_024990340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGAACC TTTTACCAGT TTGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCCCTCCT GCTGAACCGG GTCACCCCTT ACCGAGAGAA GATTTACATG 
ACCCTCTCTG CTTATATCGA GATGGAGAAC TGCACGCAGC CGGCCGTGGT CACCAAAGAC 
TTCTGCATGG TCTTCTACTC GCGCGACGCC AAGCTGCCCG CCTCGCGCTC CATCCGCAAC 
CTGTTCGGCA GTGGGAGCCT GCGGGCCTCA GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTGAGTCTGT GCCACGTGGC TGACGCGGGC AGCCCAGGCA TGCAGCGGCG GCGCCGGCGG 
GTGCTGGACA CATCCGTGGC CTACGTCCGC GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGTAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTGGAGA AGACCAGGCA CTACCTGTTG CTGCGAGAGA AGCTGGAGGC GGCCCAGCGG 
CCCGGCCCCG AGACGCTGTC CCCCGCCGCC AGTGACGGCT CCGAGGCCCA CGGCTCCTCC 
AGTGCCTCCT CCCCGCTCAC GGCTGAGGCC CGGCCAGCAT CCCTGGAGGC GCCCAGCGAG 
CGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGGCTCCTCA CCCACTCCTT CAACAGAGAG 
TACACCCACA GCCACGTCTG CGTCAGCGCC AGCGAGAGCA AGCTCTCTGA GATGTCGGTC 
ACCCTGCTGC GGGACCCGTC CATGTCCCCG CTGGGGGCCG CCACGCTCAC TCCCTCCTCT 
ACCTGCCCCT CCCTGGTCGA GGGCCGGTAT GGGGCCACCG ACCTGAGGAC CCCACAGCCC 
TGCTCCCGGC CCGCCAGCCC GGAGCCTGAG CCCCTGCCCG AGGTAGATGC CAAGAAGCTC 
CCCTCCCCTG CCCGGGCAGC GGAAGCCGAC AAGGAGCCCC AGCGCCTGCT GGTGCCTGAC 
ATCCAGGAAA TCCGTGTCAG CCCCATCGTC TCCAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTGC GGCGGCCCTA CGCCTACATG 
TACAACCACG ACAAGGACGC CGTGGAGCGC TTCGTGCTCA ACCTGTCCAC CGCCCAGGTG 
GAGTACAGCG AGGACCAGCA GGCGATGCTC AAGACCCCCA ACACGTTCGC GGTGTGCACG 
GAGCACCGGG GCATCTTGCT GCAGGCCAGC AGTGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CCCTGCTGGC CGGGACCATC CGGTCCAAGC TGTCCAGAAG GAGGTCTGCC 
CAGATGCGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846108.1
CDS	280..5454
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_024990340.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990340.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGAACC TTTTACCAGT TTGAGGCTGC GTGGGACAGC 
TCCATGCACA ACTCCCTCCT GCTGAACCGG GTCACCCCTT ACCGAGAGAA GATTTACATG 
ACCCTCTCTG CTTATATCGA GATGGAGAAC TGCACGCAGC CGGCCGTGGT CACCAAAGAC 
TTCTGCATGG TCTTCTACTC GCGCGACGCC AAGCTGCCCG CCTCGCGCTC CATCCGCAAC 
CTGTTCGGCA GTGGGAGCCT GCGGGCCTCA GAGAGCAACC GCGTGACGGG CGTGTACGAG 
CTGAGTCTGT GCCACGTGGC TGACGCGGGC AGCCCAGGCA TGCAGCGGCG GCGCCGGCGG 
GTGCTGGACA CATCCGTGGC CTACGTCCGC GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG 
CGTAGTGACA GCCTCATCCT GGACCACCAG TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG 
GAGGTGGAGA AGACCAGGCA CTACCTGTTG CTGCGAGAGA AGCTGGAGGC GGCCCAGCGG 
CCCGGCCCCG AGACGCTGTC CCCCGCCGCC AGTGACGGCT CCGAGGCCCA CGGCTCCTCC 
AGTGCCTCCT CCCCGCTCAC GGCTGAGGCC CGGCCAGCAT CCCTGGAGGC GCCCAGCGAG 
CGGCAGCGGG AGCTGGCTGT CAAGTGCTTA CGGCTCCTCA CCCACTCCTT CAACAGAGAG 
TACACCCACA GCCACGTCTG CGTCAGCGCC AGCGAGAGCA AGCTCTCTGA GATGTCGGTC 
ACCCTGCTGC GGGACCCGTC CATGTCCCCG CTGGGGGCCG CCACGCTCAC TCCCTCCTCT 
ACCTGCCCCT CCCTGGTCGA GGGCCGGTAT GGGGCCACCG ACCTGAGGAC CCCACAGCCC 
TGCTCCCGGC CCGCCAGCCC GGAGCCTGAG CCCCTGCCCG AGGTAGATGC CAAGAAGCTC 
CCCTCCCCTG CCCGGGCAGC GGAAGCCGAC AAGGAGCCCC AGCGCCTGCT GGTGCCTGAC 
ATCCAGGAAA TCCGTGTCAG CCCCATCGTC TCCAAGAAGG GCTACCTGCA CTTCCTGGAG 
CCGCACACGG CCGGCTGGGC CAAGCGCTTC GTGGTGGTGC GGCGGCCCTA CGCCTACATG 
TACAACCACG ACAAGGACGC CGTGGAGCGC TTCGTGCTCA ACCTGTCCAC CGCCCAGGTG 
GAGTACAGCG AGGACCAGCA GGCGATGCTC AAGACCCCCA ACACGTTCGC GGTGTGCACG 
GAGCACCGGG GCATCTTGCT GCAGGCCAGC AGTGACAAGG ACATGCACGA CTGGCTGTAC 
GCCTTCAACC CCCTGCTGGC CGGGACCATC CGGTCCAAGC TGTCCAGAAG GAGGTCTGCC 
CAGATGCGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_024990341.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846109.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

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CloneID	OBa233473
Clone ID Related Accession (Same CDS sequence)	XM_024990341.1
Accession Version	XM_024990341.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGGC AGTTTCTTGA TTCGGACATG CCTAGAACCT TTTACCAGTT TGAGGCTGCG 
TGGGACAGCT CCATGCACAA CTCCCTCCTG CTGAACCGGG TCACCCCTTA CCGAGAGAAG 
ATTTACATGA CCCTCTCTGC TTATATCGAG ATGGAGAACT GCACGCAGCC GGCCGTGGTC 
ACCAAAGACT TCTGCATGGT CTTCTACTCG CGCGACGCCA AGCTGCCCGC CTCGCGCTCC 
ATCCGCAACC TGTTCGGCAG TGGGAGCCTG CGGGCCTCAG AGAGCAACCG CGTGACGGGC 
GTGTACGAGC TGAGTCTGTG CCACGTGGCT GACGCGGGCA GCCCAGGCAT GCAGCGGCGG 
CGCCGGCGGG TGCTGGACAC ATCCGTGGCC TACGTCCGCG GCGAGGAGAA CCTGGCGGGC 
TGGAGGCCGC GTAGTGACAG CCTCATCCTG GACCACCAGT GGGAGCTGGA GAAGCTGAGT 
CTCCTGCAGG AGGTGGAGAA GACCAGGCAC TACCTGTTGC TGCGAGAGAA GCTGGAGGCG 
GCCCAGCGGC CCGGCCCCGA GACGCTGTCC CCCGCCGCCA GTGACGGCTC CGAGGCCCAC 
GGCTCCTCCA GTGCCTCCTC CCCGCTCACG GCTGAGGCCC GGCCAGCATC CCTGGAGGCG 
CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC AAGTGCTTAC GGCTCCTCAC CCACTCCTTC 
AACAGAGAGT ACACCCACAG CCACGTCTGC GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG 
ATGTCGGTCA CCCTGCTGCG GGACCCGTCC ATGTCCCCGC TGGGGGCCGC CACGCTCACT 
CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG GGCCGGTATG GGGCCACCGA CCTGAGGACC 
CCACAGCCCT GCTCCCGGCC CGCCAGCCCG GAGCCTGAGC CCCTGCCCGA GGTAGATGCC 
AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG GAAGCCGACA AGGAGCCCCA GCGCCTGCTG 
GTGCCTGACA TCCAGGAAAT CCGTGTCAGC CCCATCGTCT CCAAGAAGGG CTACCTGCAC 
TTCCTGGAGC CGCACACGGC CGGCTGGGCC AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC 
GCCTACATGT ACAACCACGA CAAGGACGCC GTGGAGCGCT TCGTGCTCAA CCTGTCCACC 
GCCCAGGTGG AGTACAGCGA GGACCAGCAG GCGATGCTCA AGACCCCCAA CACGTTCGCG 
GTGTGCACGG AGCACCGGGG CATCTTGCTG CAGGCCAGCA GTGACAAGGA CATGCACGAC 
TGGCTGTACG CCTTCAACCC CCTGCTGGCC GGGACCATCC GGTCCAAGCT GTCCAGAAGG 
AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846109.1
CDS	280..5403
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_024990341.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990341.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGGC AGTTTCTTGA TTCGGACATG CCTAGAACCT TTTACCAGTT TGAGGCTGCG 
TGGGACAGCT CCATGCACAA CTCCCTCCTG CTGAACCGGG TCACCCCTTA CCGAGAGAAG 
ATTTACATGA CCCTCTCTGC TTATATCGAG ATGGAGAACT GCACGCAGCC GGCCGTGGTC 
ACCAAAGACT TCTGCATGGT CTTCTACTCG CGCGACGCCA AGCTGCCCGC CTCGCGCTCC 
ATCCGCAACC TGTTCGGCAG TGGGAGCCTG CGGGCCTCAG AGAGCAACCG CGTGACGGGC 
GTGTACGAGC TGAGTCTGTG CCACGTGGCT GACGCGGGCA GCCCAGGCAT GCAGCGGCGG 
CGCCGGCGGG TGCTGGACAC ATCCGTGGCC TACGTCCGCG GCGAGGAGAA CCTGGCGGGC 
TGGAGGCCGC GTAGTGACAG CCTCATCCTG GACCACCAGT GGGAGCTGGA GAAGCTGAGT 
CTCCTGCAGG AGGTGGAGAA GACCAGGCAC TACCTGTTGC TGCGAGAGAA GCTGGAGGCG 
GCCCAGCGGC CCGGCCCCGA GACGCTGTCC CCCGCCGCCA GTGACGGCTC CGAGGCCCAC 
GGCTCCTCCA GTGCCTCCTC CCCGCTCACG GCTGAGGCCC GGCCAGCATC CCTGGAGGCG 
CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC AAGTGCTTAC GGCTCCTCAC CCACTCCTTC 
AACAGAGAGT ACACCCACAG CCACGTCTGC GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG 
ATGTCGGTCA CCCTGCTGCG GGACCCGTCC ATGTCCCCGC TGGGGGCCGC CACGCTCACT 
CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG GGCCGGTATG GGGCCACCGA CCTGAGGACC 
CCACAGCCCT GCTCCCGGCC CGCCAGCCCG GAGCCTGAGC CCCTGCCCGA GGTAGATGCC 
AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG GAAGCCGACA AGGAGCCCCA GCGCCTGCTG 
GTGCCTGACA TCCAGGAAAT CCGTGTCAGC CCCATCGTCT CCAAGAAGGG CTACCTGCAC 
TTCCTGGAGC CGCACACGGC CGGCTGGGCC AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC 
GCCTACATGT ACAACCACGA CAAGGACGCC GTGGAGCGCT TCGTGCTCAA CCTGTCCACC 
GCCCAGGTGG AGTACAGCGA GGACCAGCAG GCGATGCTCA AGACCCCCAA CACGTTCGCG 
GTGTGCACGG AGCACCGGGG CATCTTGCTG CAGGCCAGCA GTGACAAGGA CATGCACGAC 
TGGCTGTACG CCTTCAACCC CCTGCTGGCC GGGACCATCC GGTCCAAGCT GTCCAGAAGG 
AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205060.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205117.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

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CloneID	OBa233474
Clone ID Related Accession (Same CDS sequence)	XM_005205060.4
Accession Version	XM_005205060.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205060.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGAA CCTTTTACCA GTTTGAGGCT GCGTGGGACA GCTCCATGCA CAACTCCCTC 
CTGCTGAACC GGGTCACCCC TTACCGAGAG AAGATTTACA TGACCCTCTC TGCTTATATC 
GAGATGGAGA ACTGCACGCA GCCGGCCGTG GTCACCAAAG ACTTCTGCAT GGTCTTCTAC 
TCGCGCGACG CCAAGCTGCC CGCCTCGCGC TCCATCCGCA ACCTGTTCGG CAGTGGGAGC 
CTGCGGGCCT CAGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTGAGTCT GTGCCACGTG 
GCTGACGCGG GCAGCCCAGG CATGCAGCGG CGGCGCCGGC GGGTGCTGGA CACATCCGTG 
GCCTACGTCC GCGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGTAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTGGA GAAGACCAGG 
CACTACCTGT TGCTGCGAGA GAAGCTGGAG GCGGCCCAGC GGCCCGGCCC CGAGACGCTG 
TCCCCCGCCG CCAGTGACGG CTCCGAGGCC CACGGCTCCT CCAGTGCCTC CTCCCCGCTC 
ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGGCTCCT CACCCACTCC TTCAACAGAG AGTACACCCA CAGCCACGTC 
TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT GAGATGTCGG TCACCCTGCT GCGGGACCCG 
TCCATGTCCC CGCTGGGGGC CGCCACGCTC ACTCCCTCCT CTACCTGCCC CTCCCTGGTC 
GAGGGCCGGT ATGGGGCCAC CGACCTGAGG ACCCCACAGC CCTGCTCCCG GCCCGCCAGC 
CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA 
GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG CTGGTGCCTG ACATCCAGGA AATCCGTGTC 
AGCCCCATCG TCTCCAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC TACGCCTACA TGTACAACCA CGACAAGGAC 
GCCGTGGAGC GCTTCGTGCT CAACCTGTCC ACCGCCCAGG TGGAGTACAG CGAGGACCAG 
CAGGCGATGC TCAAGACCCC CAACACGTTC GCGGTGTGCA CGGAGCACCG GGGCATCTTG 
CTGCAGGCCA GCAGTGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCCCTGCTG 
GCCGGGACCA TCCGGTCCAA GCTGTCCAGA AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205117.1
CDS	280..5376
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205060.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205060.4

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGAA CCTTTTACCA GTTTGAGGCT GCGTGGGACA GCTCCATGCA CAACTCCCTC 
CTGCTGAACC GGGTCACCCC TTACCGAGAG AAGATTTACA TGACCCTCTC TGCTTATATC 
GAGATGGAGA ACTGCACGCA GCCGGCCGTG GTCACCAAAG ACTTCTGCAT GGTCTTCTAC 
TCGCGCGACG CCAAGCTGCC CGCCTCGCGC TCCATCCGCA ACCTGTTCGG CAGTGGGAGC 
CTGCGGGCCT CAGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTGAGTCT GTGCCACGTG 
GCTGACGCGG GCAGCCCAGG CATGCAGCGG CGGCGCCGGC GGGTGCTGGA CACATCCGTG 
GCCTACGTCC GCGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGTAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTGGA GAAGACCAGG 
CACTACCTGT TGCTGCGAGA GAAGCTGGAG GCGGCCCAGC GGCCCGGCCC CGAGACGCTG 
TCCCCCGCCG CCAGTGACGG CTCCGAGGCC CACGGCTCCT CCAGTGCCTC CTCCCCGCTC 
ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGGCTCCT CACCCACTCC TTCAACAGAG AGTACACCCA CAGCCACGTC 
TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT GAGATGTCGG TCACCCTGCT GCGGGACCCG 
TCCATGTCCC CGCTGGGGGC CGCCACGCTC ACTCCCTCCT CTACCTGCCC CTCCCTGGTC 
GAGGGCCGGT ATGGGGCCAC CGACCTGAGG ACCCCACAGC CCTGCTCCCG GCCCGCCAGC 
CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA 
GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG CTGGTGCCTG ACATCCAGGA AATCCGTGTC 
AGCCCCATCG TCTCCAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC TACGCCTACA TGTACAACCA CGACAAGGAC 
GCCGTGGAGC GCTTCGTGCT CAACCTGTCC ACCGCCCAGG TGGAGTACAG CGAGGACCAG 
CAGGCGATGC TCAAGACCCC CAACACGTTC GCGGTGTGCA CGGAGCACCG GGGCATCTTG 
CTGCAGGCCA GCAGTGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCCCTGCTG 
GCCGGGACCA TCCGGTCCAA GCTGTCCAGA AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205061.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205118.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa233475
Clone ID Related Accession (Same CDS sequence)	XM_005205061.4
Accession Version	XM_005205061.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205061.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GCGTTTCGTT TGGAAGTGAC 
ACTAGAACCT TTTACCAGTT TGAGGCTGCG TGGGACAGCT CCATGCACAA CTCCCTCCTG 
CTGAACCGGG TCACCCCTTA CCGAGAGAAG ATTTACATGA CCCTCTCTGC TTATATCGAG 
ATGGAGAACT GCACGCAGCC GGCCGTGGTC ACCAAAGACT TCTGCATGGT CTTCTACTCG 
CGCGACGCCA AGCTGCCCGC CTCGCGCTCC ATCCGCAACC TGTTCGGCAG TGGGAGCCTG 
CGGGCCTCAG AGAGCAACCG CGTGACGGGC GTGTACGAGC TGAGTCTGTG CCACGTGGCT 
GACGCGGGCA GCCCAGGCAT GCAGCGGCGG CGCCGGCGGG TGCTGGACAC ATCCGTGGCC 
TACGTCCGCG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GTAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTGGAGAA GACCAGGCAC 
TACCTGTTGC TGCGAGAGAA GCTGGAGGCG GCCCAGCGGC CCGGCCCCGA GACGCTGTCC 
CCCGCCGCCA GTGACGGCTC CGAGGCCCAC GGCTCCTCCA GTGCCTCCTC CCCGCTCACG 
GCTGAGGCCC GGCCAGCATC CCTGGAGGCG CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GGCTCCTCAC CCACTCCTTC AACAGAGAGT ACACCCACAG CCACGTCTGC 
GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG ATGTCGGTCA CCCTGCTGCG GGACCCGTCC 
ATGTCCCCGC TGGGGGCCGC CACGCTCACT CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG 
GGCCGGTATG GGGCCACCGA CCTGAGGACC CCACAGCCCT GCTCCCGGCC CGCCAGCCCG 
GAGCCTGAGC CCCTGCCCGA GGTAGATGCC AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG 
GAAGCCGACA AGGAGCCCCA GCGCCTGCTG GTGCCTGACA TCCAGGAAAT CCGTGTCAGC 
CCCATCGTCT CCAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC GCCTACATGT ACAACCACGA CAAGGACGCC 
GTGGAGCGCT TCGTGCTCAA CCTGTCCACC GCCCAGGTGG AGTACAGCGA GGACCAGCAG 
GCGATGCTCA AGACCCCCAA CACGTTCGCG GTGTGCACGG AGCACCGGGG CATCTTGCTG 
CAGGCCAGCA GTGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC CCTGCTGGCC 
GGGACCATCC GGTCCAAGCT GTCCAGAAGG AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205118.1
CDS	280..5373
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205061.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205061.4

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GCGTTTCGTT TGGAAGTGAC 
ACTAGAACCT TTTACCAGTT TGAGGCTGCG TGGGACAGCT CCATGCACAA CTCCCTCCTG 
CTGAACCGGG TCACCCCTTA CCGAGAGAAG ATTTACATGA CCCTCTCTGC TTATATCGAG 
ATGGAGAACT GCACGCAGCC GGCCGTGGTC ACCAAAGACT TCTGCATGGT CTTCTACTCG 
CGCGACGCCA AGCTGCCCGC CTCGCGCTCC ATCCGCAACC TGTTCGGCAG TGGGAGCCTG 
CGGGCCTCAG AGAGCAACCG CGTGACGGGC GTGTACGAGC TGAGTCTGTG CCACGTGGCT 
GACGCGGGCA GCCCAGGCAT GCAGCGGCGG CGCCGGCGGG TGCTGGACAC ATCCGTGGCC 
TACGTCCGCG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GTAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTGGAGAA GACCAGGCAC 
TACCTGTTGC TGCGAGAGAA GCTGGAGGCG GCCCAGCGGC CCGGCCCCGA GACGCTGTCC 
CCCGCCGCCA GTGACGGCTC CGAGGCCCAC GGCTCCTCCA GTGCCTCCTC CCCGCTCACG 
GCTGAGGCCC GGCCAGCATC CCTGGAGGCG CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GGCTCCTCAC CCACTCCTTC AACAGAGAGT ACACCCACAG CCACGTCTGC 
GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG ATGTCGGTCA CCCTGCTGCG GGACCCGTCC 
ATGTCCCCGC TGGGGGCCGC CACGCTCACT CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG 
GGCCGGTATG GGGCCACCGA CCTGAGGACC CCACAGCCCT GCTCCCGGCC CGCCAGCCCG 
GAGCCTGAGC CCCTGCCCGA GGTAGATGCC AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG 
GAAGCCGACA AGGAGCCCCA GCGCCTGCTG GTGCCTGACA TCCAGGAAAT CCGTGTCAGC 
CCCATCGTCT CCAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC GCCTACATGT ACAACCACGA CAAGGACGCC 
GTGGAGCGCT TCGTGCTCAA CCTGTCCACC GCCCAGGTGG AGTACAGCGA GGACCAGCAG 
GCGATGCTCA AGACCCCCAA CACGTTCGCG GTGTGCACGG AGCACCGGGG CATCTTGCTG 
CAGGCCAGCA GTGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC CCTGCTGGCC 
GGGACCATCC GGTCCAAGCT GTCCAGAAGG AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205062.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205119.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X15, mRNA.

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CloneID	OBa233476
Clone ID Related Accession (Same CDS sequence)	XM_005205062.4
Accession Version	XM_005205062.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005205062.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GAACCTTTTA CCAGTTTGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCC CTCCTGCTGA ACCGGGTCAC CCCTTACCGA 
GAGAAGATTT ACATGACCCT CTCTGCTTAT ATCGAGATGG AGAACTGCAC GCAGCCGGCC 
GTGGTCACCA AAGACTTCTG CATGGTCTTC TACTCGCGCG ACGCCAAGCT GCCCGCCTCG 
CGCTCCATCC GCAACCTGTT CGGCAGTGGG AGCCTGCGGG CCTCAGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC GTGGCTGACG CGGGCAGCCC AGGCATGCAG 
CGGCGGCGCC GGCGGGTGCT GGACACATCC GTGGCCTACG TCCGCGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGTAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT GGAGAAGACC AGGCACTACC TGTTGCTGCG AGAGAAGCTG 
GAGGCGGCCC AGCGGCCCGG CCCCGAGACG CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG 
GCCCACGGCT CCTCCAGTGC CTCCTCCCCG CTCACGGCTG AGGCCCGGCC AGCATCCCTG 
GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGGCT CCTCACCCAC 
TCCTTCAACA GAGAGTACAC CCACAGCCAC GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC 
TCTGAGATGT CGGTCACCCT GCTGCGGGAC CCGTCCATGT CCCCGCTGGG GGCCGCCACG 
CTCACTCCCT CCTCTACCTG CCCCTCCCTG GTCGAGGGCC GGTATGGGGC CACCGACCTG 
AGGACCCCAC AGCCCTGCTC CCGGCCCGCC AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA 
GATGCCAAGA AGCTCCCCTC CCCTGCCCGG GCAGCGGAAG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTGC CTGACATCCA GGAAATCCGT GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG 
CCCTACGCCT ACATGTACAA CCACGACAAG GACGCCGTGG AGCGCTTCGT GCTCAACCTG 
TCCACCGCCC AGGTGGAGTA CAGCGAGGAC CAGCAGGCGA TGCTCAAGAC CCCCAACACG 
TTCGCGGTGT GCACGGAGCA CCGGGGCATC TTGCTGCAGG CCAGCAGTGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCCCTG CTGGCCGGGA CCATCCGGTC CAAGCTGTCC 
AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205119.1
CDS	280..5349
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205062.4
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205062.4

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GAACCTTTTA CCAGTTTGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCC CTCCTGCTGA ACCGGGTCAC CCCTTACCGA 
GAGAAGATTT ACATGACCCT CTCTGCTTAT ATCGAGATGG AGAACTGCAC GCAGCCGGCC 
GTGGTCACCA AAGACTTCTG CATGGTCTTC TACTCGCGCG ACGCCAAGCT GCCCGCCTCG 
CGCTCCATCC GCAACCTGTT CGGCAGTGGG AGCCTGCGGG CCTCAGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC GTGGCTGACG CGGGCAGCCC AGGCATGCAG 
CGGCGGCGCC GGCGGGTGCT GGACACATCC GTGGCCTACG TCCGCGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGTAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT GGAGAAGACC AGGCACTACC TGTTGCTGCG AGAGAAGCTG 
GAGGCGGCCC AGCGGCCCGG CCCCGAGACG CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG 
GCCCACGGCT CCTCCAGTGC CTCCTCCCCG CTCACGGCTG AGGCCCGGCC AGCATCCCTG 
GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGGCT CCTCACCCAC 
TCCTTCAACA GAGAGTACAC CCACAGCCAC GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC 
TCTGAGATGT CGGTCACCCT GCTGCGGGAC CCGTCCATGT CCCCGCTGGG GGCCGCCACG 
CTCACTCCCT CCTCTACCTG CCCCTCCCTG GTCGAGGGCC GGTATGGGGC CACCGACCTG 
AGGACCCCAC AGCCCTGCTC CCGGCCCGCC AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA 
GATGCCAAGA AGCTCCCCTC CCCTGCCCGG GCAGCGGAAG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTGC CTGACATCCA GGAAATCCGT GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG 
CCCTACGCCT ACATGTACAA CCACGACAAG GACGCCGTGG AGCGCTTCGT GCTCAACCTG 
TCCACCGCCC AGGTGGAGTA CAGCGAGGAC CAGCAGGCGA TGCTCAAGAC CCCCAACACG 
TTCGCGGTGT GCACGGAGCA CCGGGGCATC TTGCTGCAGG CCAGCAGTGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCCCTG CTGGCCGGGA CCATCCGGTC CAAGCTGTCC 
AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803931.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802233.2 Bos taurus kinesin family member 1A (KIF1A), transcript variant X16, mRNA.

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CloneID	OBa233477
Clone ID Related Accession (Same CDS sequence)	XM_010803931.3
Accession Version	XM_010803931.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010803931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GACCTGCAGG ACGACGTCTT TCCGGAGCAC 
GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC GACCGGCCCC CCCTGTTCAG TTTAGTAGGA 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGAA CCTTTTACCA GTTTGAGGCT GCGTGGGACA GCTCCATGCA CAACTCCCTC 
CTGCTGAACC GGGTCACCCC TTACCGAGAG AAGATTTACA TGACCCTCTC TGCTTATATC 
GAGATGGAGA ACTGCACGCA GCCGGCCGTG GTCACCAAAG ACTTCTGCAT GGTCTTCTAC 
TCGCGCGACG CCAAGCTGCC CGCCTCGCGC TCCATCCGCA ACCTGTTCGG CAGTGGGAGC 
CTGCGGGCCT CAGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTGAGTCT GTGCCACGTG 
GCTGACGCGG GCAGCCCAGG CATGCAGCGG CGGCGCCGGC GGGTGCTGGA CACATCCGTG 
GCCTACGTCC GCGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGTAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTGGA GAAGACCAGG 
CACTACCTGT TGCTGCGAGA GAAGCTGGAG GCGGCCCAGC GGCCCGGCCC CGAGACGCTG 
TCCCCCGCCG CCAGTGACGG CTCCGAGGCC CACGGCTCCT CCAGTGCCTC CTCCCCGCTC 
ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGGCTCCT CACCCACTCC TTCAACAGAG AGTACACCCA CAGCCACGTC 
TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT GAGATGTCGG TCACCCTGCT GCGGGACCCG 
TCCATGTCCC CGCTGGGGGC CGCCACGCTC ACTCCCTCCT CTACCTGCCC CTCCCTGGTC 
GAGGGCCGGT ATGGGGCCAC CGACCTGAGG ACCCCACAGC CCTGCTCCCG GCCCGCCAGC 
CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA 
GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG CTGGTGCCTG ACATCCAGGA AATCCGTGTC 
AGCCCCATCG TCTCCAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC TACGCCTACA TGTACAACCA CGACAAGGAC 
GCCGTGGAGC GCTTCGTGCT CAACCTGTCC ACCGCCCAGG TGGAGTACAG CGAGGACCAG 
CAGGCGATGC TCAAGACCCC CAACACGTTC GCGGTGTGCA CGGAGCACCG GGGCATCTTG 
CTGCAGGCCA GCAGTGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCCCTGCTG 
GCCGGGACCA TCCGGTCCAA GCTGTCCAGA AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802233.2
CDS	323..5659
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803931.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803931.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAGAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GACCTGCAGG ACGACGTCTT TCCGGAGCAC 
GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC GACCGGCCCC CCCTGTTCAG TTTAGTAGGA 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGAA CCTTTTACCA GTTTGAGGCT GCGTGGGACA GCTCCATGCA CAACTCCCTC 
CTGCTGAACC GGGTCACCCC TTACCGAGAG AAGATTTACA TGACCCTCTC TGCTTATATC 
GAGATGGAGA ACTGCACGCA GCCGGCCGTG GTCACCAAAG ACTTCTGCAT GGTCTTCTAC 
TCGCGCGACG CCAAGCTGCC CGCCTCGCGC TCCATCCGCA ACCTGTTCGG CAGTGGGAGC 
CTGCGGGCCT CAGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTGAGTCT GTGCCACGTG 
GCTGACGCGG GCAGCCCAGG CATGCAGCGG CGGCGCCGGC GGGTGCTGGA CACATCCGTG 
GCCTACGTCC GCGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGTAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTGGA GAAGACCAGG 
CACTACCTGT TGCTGCGAGA GAAGCTGGAG GCGGCCCAGC GGCCCGGCCC CGAGACGCTG 
TCCCCCGCCG CCAGTGACGG CTCCGAGGCC CACGGCTCCT CCAGTGCCTC CTCCCCGCTC 
ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGGCTCCT CACCCACTCC TTCAACAGAG AGTACACCCA CAGCCACGTC 
TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT GAGATGTCGG TCACCCTGCT GCGGGACCCG 
TCCATGTCCC CGCTGGGGGC CGCCACGCTC ACTCCCTCCT CTACCTGCCC CTCCCTGGTC 
GAGGGCCGGT ATGGGGCCAC CGACCTGAGG ACCCCACAGC CCTGCTCCCG GCCCGCCAGC 
CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA 
GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG CTGGTGCCTG ACATCCAGGA AATCCGTGTC 
AGCCCCATCG TCTCCAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC TACGCCTACA TGTACAACCA CGACAAGGAC 
GCCGTGGAGC GCTTCGTGCT CAACCTGTCC ACCGCCCAGG TGGAGTACAG CGAGGACCAG 
CAGGCGATGC TCAAGACCCC CAACACGTTC GCGGTGTGCA CGGAGCACCG GGGCATCTTG 
CTGCAGGCCA GCAGTGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCCCTGCTG 
GCCGGGACCA TCCGGTCCAA GCTGTCCAGA AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462892.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318378.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

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CloneID	OBa18299
Clone ID Related Accession (Same CDS sequence)	XM_015462892.1 , XM_005205060.3
Accession Version	XM_015462892.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGAA CCTTTTACCA GTTTGAGGCT GCGTGGGACA GCTCCATGCA CAACTCCCTC 
CTGCTGAACC GGGTCACCCC TTACCGAGAG AAGATTTACA TGACCCTCTC TGCTTATATC 
GAGATGGAGA ACTGCACGCA GCCGGCCGTG GTCACCAAAG ACTTCTGCAT GGTCTTCTAC 
TCGCGCGACG CCAAGCTGCC CGCCTCGCGC TCCATCCGCA ACCTGTTCGG CAGTGGGAGC 
CTGCGGGCCT CAGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTGAGTCT GTGCCACGTG 
GCTGACGCGG GCAGCCCAGG CATGCAGCGG CGGCGCCGGC GGGTGCTGGA CACATCCGTG 
GCCTACGTCC GCGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGTAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTGGA GAAGACCAGG 
CACTACCTGT TGCTGCGAGA GAAGCTGGAG GCGGCCCAGC GGCCCGGCCC CGAGACGCTG 
TCCCCCGCCG CCAGTGACGG CTCCGAGGCC CACGGCTCCT CCAGTGCCTC CTCCCCGCTC 
ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGGCTCCT CACCCACTCC TTCAACAGAG AGTACACCCA CAGCCACGTC 
TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT GAGATGTCGG TCACCCTGCT GCGGGACCCG 
TCCATGTCCC CGCTGGGGGC CGCCACGCTC ACTCCCTCCT CTACCTGCCC CTCCCTGGTC 
GAGGGCCGGT ATGGGGCCAC CGACCTGAGG ACCCCACAGC CCTGCTCCCG GCCCGCCAGC 
CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA 
GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG CTGGTGCCTG ACATCCAGGA AATCCGTGTC 
AGCCCCATCG TCTCCAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC TACGCCTACA TGTACAACCA CGACAAGGAC 
GCCGTGGAGC GCTTCGTGCT CAACCTGTCC ACCGCCCAGG TGGAGTACAG CGAGGACCAG 
CAGGCGATGC TCAAGACCCC CAACACGTTC GCGGTGTGCA CGGAGCACCG GGGCATCTTG 
CTGCAGGCCA GCAGTGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCCCTGCTG 
GCCGGGACCA TCCGGTCCAA GCTGTCCAGA AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318378.1
CDS	323..5419
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462892.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462892.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGAA CCTTTTACCA GTTTGAGGCT GCGTGGGACA GCTCCATGCA CAACTCCCTC 
CTGCTGAACC GGGTCACCCC TTACCGAGAG AAGATTTACA TGACCCTCTC TGCTTATATC 
GAGATGGAGA ACTGCACGCA GCCGGCCGTG GTCACCAAAG ACTTCTGCAT GGTCTTCTAC 
TCGCGCGACG CCAAGCTGCC CGCCTCGCGC TCCATCCGCA ACCTGTTCGG CAGTGGGAGC 
CTGCGGGCCT CAGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTGAGTCT GTGCCACGTG 
GCTGACGCGG GCAGCCCAGG CATGCAGCGG CGGCGCCGGC GGGTGCTGGA CACATCCGTG 
GCCTACGTCC GCGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGTAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTGGA GAAGACCAGG 
CACTACCTGT TGCTGCGAGA GAAGCTGGAG GCGGCCCAGC GGCCCGGCCC CGAGACGCTG 
TCCCCCGCCG CCAGTGACGG CTCCGAGGCC CACGGCTCCT CCAGTGCCTC CTCCCCGCTC 
ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGGCTCCT CACCCACTCC TTCAACAGAG AGTACACCCA CAGCCACGTC 
TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT GAGATGTCGG TCACCCTGCT GCGGGACCCG 
TCCATGTCCC CGCTGGGGGC CGCCACGCTC ACTCCCTCCT CTACCTGCCC CTCCCTGGTC 
GAGGGCCGGT ATGGGGCCAC CGACCTGAGG ACCCCACAGC CCTGCTCCCG GCCCGCCAGC 
CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA 
GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG CTGGTGCCTG ACATCCAGGA AATCCGTGTC 
AGCCCCATCG TCTCCAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC TACGCCTACA TGTACAACCA CGACAAGGAC 
GCCGTGGAGC GCTTCGTGCT CAACCTGTCC ACCGCCCAGG TGGAGTACAG CGAGGACCAG 
CAGGCGATGC TCAAGACCCC CAACACGTTC GCGGTGTGCA CGGAGCACCG GGGCATCTTG 
CTGCAGGCCA GCAGTGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCCCTGCTG 
GCCGGGACCA TCCGGTCCAA GCTGTCCAGA AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205060.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205117.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

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CloneID	OBa18299
Clone ID Related Accession (Same CDS sequence)	XM_015462892.1 , XM_005205060.3
Accession Version	XM_005205060.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205060.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGAA CCTTTTACCA GTTTGAGGCT GCGTGGGACA GCTCCATGCA CAACTCCCTC 
CTGCTGAACC GGGTCACCCC TTACCGAGAG AAGATTTACA TGACCCTCTC TGCTTATATC 
GAGATGGAGA ACTGCACGCA GCCGGCCGTG GTCACCAAAG ACTTCTGCAT GGTCTTCTAC 
TCGCGCGACG CCAAGCTGCC CGCCTCGCGC TCCATCCGCA ACCTGTTCGG CAGTGGGAGC 
CTGCGGGCCT CAGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTGAGTCT GTGCCACGTG 
GCTGACGCGG GCAGCCCAGG CATGCAGCGG CGGCGCCGGC GGGTGCTGGA CACATCCGTG 
GCCTACGTCC GCGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGTAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTGGA GAAGACCAGG 
CACTACCTGT TGCTGCGAGA GAAGCTGGAG GCGGCCCAGC GGCCCGGCCC CGAGACGCTG 
TCCCCCGCCG CCAGTGACGG CTCCGAGGCC CACGGCTCCT CCAGTGCCTC CTCCCCGCTC 
ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGGCTCCT CACCCACTCC TTCAACAGAG AGTACACCCA CAGCCACGTC 
TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT GAGATGTCGG TCACCCTGCT GCGGGACCCG 
TCCATGTCCC CGCTGGGGGC CGCCACGCTC ACTCCCTCCT CTACCTGCCC CTCCCTGGTC 
GAGGGCCGGT ATGGGGCCAC CGACCTGAGG ACCCCACAGC CCTGCTCCCG GCCCGCCAGC 
CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA 
GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG CTGGTGCCTG ACATCCAGGA AATCCGTGTC 
AGCCCCATCG TCTCCAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC TACGCCTACA TGTACAACCA CGACAAGGAC 
GCCGTGGAGC GCTTCGTGCT CAACCTGTCC ACCGCCCAGG TGGAGTACAG CGAGGACCAG 
CAGGCGATGC TCAAGACCCC CAACACGTTC GCGGTGTGCA CGGAGCACCG GGGCATCTTG 
CTGCAGGCCA GCAGTGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCCCTGCTG 
GCCGGGACCA TCCGGTCCAA GCTGTCCAGA AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205117.1
CDS	323..5419
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205060.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205060.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGGGCCTTCG TGTACCTCAG CAACCTGCTG TACCCCGTGC CGCTGGTGCA CCGCGTGGCC 
GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC TTCCTGCGCG TGGCCGTGCA GGCCATCTCA 
GCCGACGAGG AGGCCCCTGA CTACGGCTCC GGCGTCCGCC AGTCGGGAAC AGCCAAAATC 
TCCTTTGACG ACCAGCATTT TGAGAAGTTC CAGGCCGAGT CCTGCCCCGG GGTGGGCATG 
TCCCGCTCAG GGACCTCCCA GGAGGAGCTG CGGATTGTGG AAGGCCAGGG CCAGGCCGCA 
GACTCCGGGC CCTCAGCCGA CGAGGTCAAC AACAACACCT GTTCAGCGGT GACCCCGGAA 
GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC CTGGATGGGC CCCTGGACGC CGCCCTGGAC 
CACCTCGGCC TGGGCAGCAC CTTCACCTTC CGCGTGACCG TCCTGCAGGC GTCCAGCATA 
TCCGCTGAAT ATGCCGACAT CTTCTGCCAG TTCAACTTCA TCCATCGCCA TGACGAGGCC 
TTCTCTACGG AGCCCCTGAA GAACACGGGG AGGGGACCCC CGCTCGGCTT CTACCACGTC 
CAAAACATTG CGGTGGAGGT GACCAGGTCC TTCATCGAGT ACATCAAGAG CCAGCCCCTC 
GTGTTCGAGG TCTTCGGGCA CTACCAGCAG CACCCGTTCC CGCCCCTCTG CAAGGACGTG 
CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC TTCCCACGGG TCATGCCGCT GTCCAAGCCA 
GTGCCGGCCA CCAAGCTCAG CACGCTGACG CGACCCTGCC CGGGCCCCTG CCACTGCAAG 
TACGACCTGC TGGTCTACTT CGAGATCTGC GAGCTGGAGG CCAACGGCGA CTACATCCCC 
GCAGTGGTGG ATCACCGCGG CGGGATGCCG TGCATGGGGA CCTTCCTGCT CCACCAGGGC 
ATCCAGAGAC GGATCACCGT GACCCTGCTG CACGAGACGG GCAGCCACAT CCGCTGGAAG 
GAAGTGCGGG AGCTGGTGGT GGGTCGCATC CGAAACACCC CGGAAACGGA CGAGTCCCTG 
ATTGACCCTA ATATCTTGTC GCTCAGCATC CTCTCTTCTG GATACATCTG CCCAGCCCAG 
GATGACCGAA CCTTTTACCA GTTTGAGGCT GCGTGGGACA GCTCCATGCA CAACTCCCTC 
CTGCTGAACC GGGTCACCCC TTACCGAGAG AAGATTTACA TGACCCTCTC TGCTTATATC 
GAGATGGAGA ACTGCACGCA GCCGGCCGTG GTCACCAAAG ACTTCTGCAT GGTCTTCTAC 
TCGCGCGACG CCAAGCTGCC CGCCTCGCGC TCCATCCGCA ACCTGTTCGG CAGTGGGAGC 
CTGCGGGCCT CAGAGAGCAA CCGCGTGACG GGCGTGTACG AGCTGAGTCT GTGCCACGTG 
GCTGACGCGG GCAGCCCAGG CATGCAGCGG CGGCGCCGGC GGGTGCTGGA CACATCCGTG 
GCCTACGTCC GCGGCGAGGA GAACCTGGCG GGCTGGAGGC CGCGTAGTGA CAGCCTCATC 
CTGGACCACC AGTGGGAGCT GGAGAAGCTG AGTCTCCTGC AGGAGGTGGA GAAGACCAGG 
CACTACCTGT TGCTGCGAGA GAAGCTGGAG GCGGCCCAGC GGCCCGGCCC CGAGACGCTG 
TCCCCCGCCG CCAGTGACGG CTCCGAGGCC CACGGCTCCT CCAGTGCCTC CTCCCCGCTC 
ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT 
GTCAAGTGCT TACGGCTCCT CACCCACTCC TTCAACAGAG AGTACACCCA CAGCCACGTC 
TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT GAGATGTCGG TCACCCTGCT GCGGGACCCG 
TCCATGTCCC CGCTGGGGGC CGCCACGCTC ACTCCCTCCT CTACCTGCCC CTCCCTGGTC 
GAGGGCCGGT ATGGGGCCAC CGACCTGAGG ACCCCACAGC CCTGCTCCCG GCCCGCCAGC 
CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA 
GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG CTGGTGCCTG ACATCCAGGA AATCCGTGTC 
AGCCCCATCG TCTCCAAGAA GGGCTACCTG CACTTCCTGG AGCCGCACAC GGCCGGCTGG 
GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC TACGCCTACA TGTACAACCA CGACAAGGAC 
GCCGTGGAGC GCTTCGTGCT CAACCTGTCC ACCGCCCAGG TGGAGTACAG CGAGGACCAG 
CAGGCGATGC TCAAGACCCC CAACACGTTC GCGGTGTGCA CGGAGCACCG GGGCATCTTG 
CTGCAGGCCA GCAGTGACAA GGACATGCAC GACTGGCTGT ACGCCTTCAA CCCCCTGCTG 
GCCGGGACCA TCCGGTCCAA GCTGTCCAGA AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205061.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205118.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

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CloneID	OBa18300
Clone ID Related Accession (Same CDS sequence)	XM_005205061.3 , XM_015462893.1
Accession Version	XM_005205061.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GCGTTTCGTT TGGAAGTGAC 
ACTAGAACCT TTTACCAGTT TGAGGCTGCG TGGGACAGCT CCATGCACAA CTCCCTCCTG 
CTGAACCGGG TCACCCCTTA CCGAGAGAAG ATTTACATGA CCCTCTCTGC TTATATCGAG 
ATGGAGAACT GCACGCAGCC GGCCGTGGTC ACCAAAGACT TCTGCATGGT CTTCTACTCG 
CGCGACGCCA AGCTGCCCGC CTCGCGCTCC ATCCGCAACC TGTTCGGCAG TGGGAGCCTG 
CGGGCCTCAG AGAGCAACCG CGTGACGGGC GTGTACGAGC TGAGTCTGTG CCACGTGGCT 
GACGCGGGCA GCCCAGGCAT GCAGCGGCGG CGCCGGCGGG TGCTGGACAC ATCCGTGGCC 
TACGTCCGCG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GTAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTGGAGAA GACCAGGCAC 
TACCTGTTGC TGCGAGAGAA GCTGGAGGCG GCCCAGCGGC CCGGCCCCGA GACGCTGTCC 
CCCGCCGCCA GTGACGGCTC CGAGGCCCAC GGCTCCTCCA GTGCCTCCTC CCCGCTCACG 
GCTGAGGCCC GGCCAGCATC CCTGGAGGCG CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GGCTCCTCAC CCACTCCTTC AACAGAGAGT ACACCCACAG CCACGTCTGC 
GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG ATGTCGGTCA CCCTGCTGCG GGACCCGTCC 
ATGTCCCCGC TGGGGGCCGC CACGCTCACT CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG 
GGCCGGTATG GGGCCACCGA CCTGAGGACC CCACAGCCCT GCTCCCGGCC CGCCAGCCCG 
GAGCCTGAGC CCCTGCCCGA GGTAGATGCC AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG 
GAAGCCGACA AGGAGCCCCA GCGCCTGCTG GTGCCTGACA TCCAGGAAAT CCGTGTCAGC 
CCCATCGTCT CCAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC GCCTACATGT ACAACCACGA CAAGGACGCC 
GTGGAGCGCT TCGTGCTCAA CCTGTCCACC GCCCAGGTGG AGTACAGCGA GGACCAGCAG 
GCGATGCTCA AGACCCCCAA CACGTTCGCG GTGTGCACGG AGCACCGGGG CATCTTGCTG 
CAGGCCAGCA GTGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC CCTGCTGGCC 
GGGACCATCC GGTCCAAGCT GTCCAGAAGG AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205118.1
CDS	323..5416
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205061.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205061.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GCGTTTCGTT TGGAAGTGAC 
ACTAGAACCT TTTACCAGTT TGAGGCTGCG TGGGACAGCT CCATGCACAA CTCCCTCCTG 
CTGAACCGGG TCACCCCTTA CCGAGAGAAG ATTTACATGA CCCTCTCTGC TTATATCGAG 
ATGGAGAACT GCACGCAGCC GGCCGTGGTC ACCAAAGACT TCTGCATGGT CTTCTACTCG 
CGCGACGCCA AGCTGCCCGC CTCGCGCTCC ATCCGCAACC TGTTCGGCAG TGGGAGCCTG 
CGGGCCTCAG AGAGCAACCG CGTGACGGGC GTGTACGAGC TGAGTCTGTG CCACGTGGCT 
GACGCGGGCA GCCCAGGCAT GCAGCGGCGG CGCCGGCGGG TGCTGGACAC ATCCGTGGCC 
TACGTCCGCG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GTAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTGGAGAA GACCAGGCAC 
TACCTGTTGC TGCGAGAGAA GCTGGAGGCG GCCCAGCGGC CCGGCCCCGA GACGCTGTCC 
CCCGCCGCCA GTGACGGCTC CGAGGCCCAC GGCTCCTCCA GTGCCTCCTC CCCGCTCACG 
GCTGAGGCCC GGCCAGCATC CCTGGAGGCG CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GGCTCCTCAC CCACTCCTTC AACAGAGAGT ACACCCACAG CCACGTCTGC 
GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG ATGTCGGTCA CCCTGCTGCG GGACCCGTCC 
ATGTCCCCGC TGGGGGCCGC CACGCTCACT CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG 
GGCCGGTATG GGGCCACCGA CCTGAGGACC CCACAGCCCT GCTCCCGGCC CGCCAGCCCG 
GAGCCTGAGC CCCTGCCCGA GGTAGATGCC AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG 
GAAGCCGACA AGGAGCCCCA GCGCCTGCTG GTGCCTGACA TCCAGGAAAT CCGTGTCAGC 
CCCATCGTCT CCAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC GCCTACATGT ACAACCACGA CAAGGACGCC 
GTGGAGCGCT TCGTGCTCAA CCTGTCCACC GCCCAGGTGG AGTACAGCGA GGACCAGCAG 
GCGATGCTCA AGACCCCCAA CACGTTCGCG GTGTGCACGG AGCACCGGGG CATCTTGCTG 
CAGGCCAGCA GTGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC CCTGCTGGCC 
GGGACCATCC GGTCCAAGCT GTCCAGAAGG AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462893.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318379.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

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CloneID	OBa18300
Clone ID Related Accession (Same CDS sequence)	XM_005205061.3 , XM_015462893.1
Accession Version	XM_015462893.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GCGTTTCGTT TGGAAGTGAC 
ACTAGAACCT TTTACCAGTT TGAGGCTGCG TGGGACAGCT CCATGCACAA CTCCCTCCTG 
CTGAACCGGG TCACCCCTTA CCGAGAGAAG ATTTACATGA CCCTCTCTGC TTATATCGAG 
ATGGAGAACT GCACGCAGCC GGCCGTGGTC ACCAAAGACT TCTGCATGGT CTTCTACTCG 
CGCGACGCCA AGCTGCCCGC CTCGCGCTCC ATCCGCAACC TGTTCGGCAG TGGGAGCCTG 
CGGGCCTCAG AGAGCAACCG CGTGACGGGC GTGTACGAGC TGAGTCTGTG CCACGTGGCT 
GACGCGGGCA GCCCAGGCAT GCAGCGGCGG CGCCGGCGGG TGCTGGACAC ATCCGTGGCC 
TACGTCCGCG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GTAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTGGAGAA GACCAGGCAC 
TACCTGTTGC TGCGAGAGAA GCTGGAGGCG GCCCAGCGGC CCGGCCCCGA GACGCTGTCC 
CCCGCCGCCA GTGACGGCTC CGAGGCCCAC GGCTCCTCCA GTGCCTCCTC CCCGCTCACG 
GCTGAGGCCC GGCCAGCATC CCTGGAGGCG CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GGCTCCTCAC CCACTCCTTC AACAGAGAGT ACACCCACAG CCACGTCTGC 
GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG ATGTCGGTCA CCCTGCTGCG GGACCCGTCC 
ATGTCCCCGC TGGGGGCCGC CACGCTCACT CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG 
GGCCGGTATG GGGCCACCGA CCTGAGGACC CCACAGCCCT GCTCCCGGCC CGCCAGCCCG 
GAGCCTGAGC CCCTGCCCGA GGTAGATGCC AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG 
GAAGCCGACA AGGAGCCCCA GCGCCTGCTG GTGCCTGACA TCCAGGAAAT CCGTGTCAGC 
CCCATCGTCT CCAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC GCCTACATGT ACAACCACGA CAAGGACGCC 
GTGGAGCGCT TCGTGCTCAA CCTGTCCACC GCCCAGGTGG AGTACAGCGA GGACCAGCAG 
GCGATGCTCA AGACCCCCAA CACGTTCGCG GTGTGCACGG AGCACCGGGG CATCTTGCTG 
CAGGCCAGCA GTGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC CCTGCTGGCC 
GGGACCATCC GGTCCAAGCT GTCCAGAAGG AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318379.1
CDS	323..5416
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462893.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462893.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GCGTTTCGTT TGGAAGTGAC 
ACTAGAACCT TTTACCAGTT TGAGGCTGCG TGGGACAGCT CCATGCACAA CTCCCTCCTG 
CTGAACCGGG TCACCCCTTA CCGAGAGAAG ATTTACATGA CCCTCTCTGC TTATATCGAG 
ATGGAGAACT GCACGCAGCC GGCCGTGGTC ACCAAAGACT TCTGCATGGT CTTCTACTCG 
CGCGACGCCA AGCTGCCCGC CTCGCGCTCC ATCCGCAACC TGTTCGGCAG TGGGAGCCTG 
CGGGCCTCAG AGAGCAACCG CGTGACGGGC GTGTACGAGC TGAGTCTGTG CCACGTGGCT 
GACGCGGGCA GCCCAGGCAT GCAGCGGCGG CGCCGGCGGG TGCTGGACAC ATCCGTGGCC 
TACGTCCGCG GCGAGGAGAA CCTGGCGGGC TGGAGGCCGC GTAGTGACAG CCTCATCCTG 
GACCACCAGT GGGAGCTGGA GAAGCTGAGT CTCCTGCAGG AGGTGGAGAA GACCAGGCAC 
TACCTGTTGC TGCGAGAGAA GCTGGAGGCG GCCCAGCGGC CCGGCCCCGA GACGCTGTCC 
CCCGCCGCCA GTGACGGCTC CGAGGCCCAC GGCTCCTCCA GTGCCTCCTC CCCGCTCACG 
GCTGAGGCCC GGCCAGCATC CCTGGAGGCG CCCAGCGAGC GGCAGCGGGA GCTGGCTGTC 
AAGTGCTTAC GGCTCCTCAC CCACTCCTTC AACAGAGAGT ACACCCACAG CCACGTCTGC 
GTCAGCGCCA GCGAGAGCAA GCTCTCTGAG ATGTCGGTCA CCCTGCTGCG GGACCCGTCC 
ATGTCCCCGC TGGGGGCCGC CACGCTCACT CCCTCCTCTA CCTGCCCCTC CCTGGTCGAG 
GGCCGGTATG GGGCCACCGA CCTGAGGACC CCACAGCCCT GCTCCCGGCC CGCCAGCCCG 
GAGCCTGAGC CCCTGCCCGA GGTAGATGCC AAGAAGCTCC CCTCCCCTGC CCGGGCAGCG 
GAAGCCGACA AGGAGCCCCA GCGCCTGCTG GTGCCTGACA TCCAGGAAAT CCGTGTCAGC 
CCCATCGTCT CCAAGAAGGG CTACCTGCAC TTCCTGGAGC CGCACACGGC CGGCTGGGCC 
AAGCGCTTCG TGGTGGTGCG GCGGCCCTAC GCCTACATGT ACAACCACGA CAAGGACGCC 
GTGGAGCGCT TCGTGCTCAA CCTGTCCACC GCCCAGGTGG AGTACAGCGA GGACCAGCAG 
GCGATGCTCA AGACCCCCAA CACGTTCGCG GTGTGCACGG AGCACCGGGG CATCTTGCTG 
CAGGCCAGCA GTGACAAGGA CATGCACGAC TGGCTGTACG CCTTCAACCC CCTGCTGGCC 
GGGACCATCC GGTCCAAGCT GTCCAGAAGG AGGTCTGCCC AGATGCGGGT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205062.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205119.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

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CloneID	OBa18301
Clone ID Related Accession (Same CDS sequence)	XM_005205062.3 , XM_015462894.1
Accession Version	XM_005205062.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205062.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GAACCTTTTA CCAGTTTGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCC CTCCTGCTGA ACCGGGTCAC CCCTTACCGA 
GAGAAGATTT ACATGACCCT CTCTGCTTAT ATCGAGATGG AGAACTGCAC GCAGCCGGCC 
GTGGTCACCA AAGACTTCTG CATGGTCTTC TACTCGCGCG ACGCCAAGCT GCCCGCCTCG 
CGCTCCATCC GCAACCTGTT CGGCAGTGGG AGCCTGCGGG CCTCAGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC GTGGCTGACG CGGGCAGCCC AGGCATGCAG 
CGGCGGCGCC GGCGGGTGCT GGACACATCC GTGGCCTACG TCCGCGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGTAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT GGAGAAGACC AGGCACTACC TGTTGCTGCG AGAGAAGCTG 
GAGGCGGCCC AGCGGCCCGG CCCCGAGACG CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG 
GCCCACGGCT CCTCCAGTGC CTCCTCCCCG CTCACGGCTG AGGCCCGGCC AGCATCCCTG 
GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGGCT CCTCACCCAC 
TCCTTCAACA GAGAGTACAC CCACAGCCAC GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC 
TCTGAGATGT CGGTCACCCT GCTGCGGGAC CCGTCCATGT CCCCGCTGGG GGCCGCCACG 
CTCACTCCCT CCTCTACCTG CCCCTCCCTG GTCGAGGGCC GGTATGGGGC CACCGACCTG 
AGGACCCCAC AGCCCTGCTC CCGGCCCGCC AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA 
GATGCCAAGA AGCTCCCCTC CCCTGCCCGG GCAGCGGAAG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTGC CTGACATCCA GGAAATCCGT GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG 
CCCTACGCCT ACATGTACAA CCACGACAAG GACGCCGTGG AGCGCTTCGT GCTCAACCTG 
TCCACCGCCC AGGTGGAGTA CAGCGAGGAC CAGCAGGCGA TGCTCAAGAC CCCCAACACG 
TTCGCGGTGT GCACGGAGCA CCGGGGCATC TTGCTGCAGG CCAGCAGTGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCCCTG CTGGCCGGGA CCATCCGGTC CAAGCTGTCC 
AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205119.1
CDS	323..5392
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205062.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205062.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GAACCTTTTA CCAGTTTGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCC CTCCTGCTGA ACCGGGTCAC CCCTTACCGA 
GAGAAGATTT ACATGACCCT CTCTGCTTAT ATCGAGATGG AGAACTGCAC GCAGCCGGCC 
GTGGTCACCA AAGACTTCTG CATGGTCTTC TACTCGCGCG ACGCCAAGCT GCCCGCCTCG 
CGCTCCATCC GCAACCTGTT CGGCAGTGGG AGCCTGCGGG CCTCAGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC GTGGCTGACG CGGGCAGCCC AGGCATGCAG 
CGGCGGCGCC GGCGGGTGCT GGACACATCC GTGGCCTACG TCCGCGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGTAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT GGAGAAGACC AGGCACTACC TGTTGCTGCG AGAGAAGCTG 
GAGGCGGCCC AGCGGCCCGG CCCCGAGACG CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG 
GCCCACGGCT CCTCCAGTGC CTCCTCCCCG CTCACGGCTG AGGCCCGGCC AGCATCCCTG 
GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGGCT CCTCACCCAC 
TCCTTCAACA GAGAGTACAC CCACAGCCAC GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC 
TCTGAGATGT CGGTCACCCT GCTGCGGGAC CCGTCCATGT CCCCGCTGGG GGCCGCCACG 
CTCACTCCCT CCTCTACCTG CCCCTCCCTG GTCGAGGGCC GGTATGGGGC CACCGACCTG 
AGGACCCCAC AGCCCTGCTC CCGGCCCGCC AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA 
GATGCCAAGA AGCTCCCCTC CCCTGCCCGG GCAGCGGAAG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTGC CTGACATCCA GGAAATCCGT GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG 
CCCTACGCCT ACATGTACAA CCACGACAAG GACGCCGTGG AGCGCTTCGT GCTCAACCTG 
TCCACCGCCC AGGTGGAGTA CAGCGAGGAC CAGCAGGCGA TGCTCAAGAC CCCCAACACG 
TTCGCGGTGT GCACGGAGCA CCGGGGCATC TTGCTGCAGG CCAGCAGTGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCCCTG CTGGCCGGGA CCATCCGGTC CAAGCTGTCC 
AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462894.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318380.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa18301
Clone ID Related Accession (Same CDS sequence)	XM_005205062.3 , XM_015462894.1
Accession Version	XM_015462894.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GAACCTTTTA CCAGTTTGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCC CTCCTGCTGA ACCGGGTCAC CCCTTACCGA 
GAGAAGATTT ACATGACCCT CTCTGCTTAT ATCGAGATGG AGAACTGCAC GCAGCCGGCC 
GTGGTCACCA AAGACTTCTG CATGGTCTTC TACTCGCGCG ACGCCAAGCT GCCCGCCTCG 
CGCTCCATCC GCAACCTGTT CGGCAGTGGG AGCCTGCGGG CCTCAGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC GTGGCTGACG CGGGCAGCCC AGGCATGCAG 
CGGCGGCGCC GGCGGGTGCT GGACACATCC GTGGCCTACG TCCGCGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGTAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT GGAGAAGACC AGGCACTACC TGTTGCTGCG AGAGAAGCTG 
GAGGCGGCCC AGCGGCCCGG CCCCGAGACG CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG 
GCCCACGGCT CCTCCAGTGC CTCCTCCCCG CTCACGGCTG AGGCCCGGCC AGCATCCCTG 
GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGGCT CCTCACCCAC 
TCCTTCAACA GAGAGTACAC CCACAGCCAC GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC 
TCTGAGATGT CGGTCACCCT GCTGCGGGAC CCGTCCATGT CCCCGCTGGG GGCCGCCACG 
CTCACTCCCT CCTCTACCTG CCCCTCCCTG GTCGAGGGCC GGTATGGGGC CACCGACCTG 
AGGACCCCAC AGCCCTGCTC CCGGCCCGCC AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA 
GATGCCAAGA AGCTCCCCTC CCCTGCCCGG GCAGCGGAAG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTGC CTGACATCCA GGAAATCCGT GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG 
CCCTACGCCT ACATGTACAA CCACGACAAG GACGCCGTGG AGCGCTTCGT GCTCAACCTG 
TCCACCGCCC AGGTGGAGTA CAGCGAGGAC CAGCAGGCGA TGCTCAAGAC CCCCAACACG 
TTCGCGGTGT GCACGGAGCA CCGGGGCATC TTGCTGCAGG CCAGCAGTGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCCCTG CTGGCCGGGA CCATCCGGTC CAAGCTGTCC 
AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318380.1
CDS	323..5392
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462894.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462894.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGGGCCT TCGTGTACCT CAGCAACCTG 
CTGTACCCCG TGCCGCTGGT GCACCGCGTG GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG 
GGCTTCCTGC GCGTGGCCGT GCAGGCCATC TCAGCCGACG AGGAGGCCCC TGACTACGGC 
TCCGGCGTCC GCCAGTCGGG AACAGCCAAA ATCTCCTTTG ACGACCAGCA TTTTGAGAAG 
TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC ATGTCCCGCT CAGGGACCTC CCAGGAGGAG 
CTGCGGATTG TGGAAGGCCA GGGCCAGGCC GCAGACTCCG GGCCCTCAGC CGACGAGGTC 
AACAACAACA CCTGTTCAGC GGTGACCCCG GAAGGCCTCC TGGACAGCCC TGAGAAAGCC 
GCCCTGGATG GGCCCCTGGA CGCCGCCCTG GACCACCTCG GCCTGGGCAG CACCTTCACC 
TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC ATATCCGCTG AATATGCCGA CATCTTCTGC 
CAGTTCAACT TCATCCATCG CCATGACGAG GCCTTCTCTA CGGAGCCCCT GAAGAACACG 
GGGAGGGGAC CCCCGCTCGG CTTCTACCAC GTCCAAAACA TTGCGGTGGA GGTGACCAGG 
TCCTTCATCG AGTACATCAA GAGCCAGCCC CTCGTGTTCG AGGTCTTCGG GCACTACCAG 
CAGCACCCGT TCCCGCCCCT CTGCAAGGAC GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC 
CACTTCCCAC GGGTCATGCC GCTGTCCAAG CCAGTGCCGG CCACCAAGCT CAGCACGCTG 
ACGCGACCCT GCCCGGGCCC CTGCCACTGC AAGTACGACC TGCTGGTCTA CTTCGAGATC 
TGCGAGCTGG AGGCCAACGG CGACTACATC CCCGCAGTGG TGGATCACCG CGGCGGGATG 
CCGTGCATGG GGACCTTCCT GCTCCACCAG GGCATCCAGA GACGGATCAC CGTGACCCTG 
CTGCACGAGA CGGGCAGCCA CATCCGCTGG AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC 
ATCCGAAACA CCCCGGAAAC GGACGAGTCC CTGATTGACC CTAATATCTT GTCGCTCAGC 
ATCCTCTCTT CTGGATACAT CTGCCCAGCC CAGGATGACC GAACCTTTTA CCAGTTTGAG 
GCTGCGTGGG ACAGCTCCAT GCACAACTCC CTCCTGCTGA ACCGGGTCAC CCCTTACCGA 
GAGAAGATTT ACATGACCCT CTCTGCTTAT ATCGAGATGG AGAACTGCAC GCAGCCGGCC 
GTGGTCACCA AAGACTTCTG CATGGTCTTC TACTCGCGCG ACGCCAAGCT GCCCGCCTCG 
CGCTCCATCC GCAACCTGTT CGGCAGTGGG AGCCTGCGGG CCTCAGAGAG CAACCGCGTG 
ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC GTGGCTGACG CGGGCAGCCC AGGCATGCAG 
CGGCGGCGCC GGCGGGTGCT GGACACATCC GTGGCCTACG TCCGCGGCGA GGAGAACCTG 
GCGGGCTGGA GGCCGCGTAG TGACAGCCTC ATCCTGGACC ACCAGTGGGA GCTGGAGAAG 
CTGAGTCTCC TGCAGGAGGT GGAGAAGACC AGGCACTACC TGTTGCTGCG AGAGAAGCTG 
GAGGCGGCCC AGCGGCCCGG CCCCGAGACG CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG 
GCCCACGGCT CCTCCAGTGC CTCCTCCCCG CTCACGGCTG AGGCCCGGCC AGCATCCCTG 
GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG GCTGTCAAGT GCTTACGGCT CCTCACCCAC 
TCCTTCAACA GAGAGTACAC CCACAGCCAC GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC 
TCTGAGATGT CGGTCACCCT GCTGCGGGAC CCGTCCATGT CCCCGCTGGG GGCCGCCACG 
CTCACTCCCT CCTCTACCTG CCCCTCCCTG GTCGAGGGCC GGTATGGGGC CACCGACCTG 
AGGACCCCAC AGCCCTGCTC CCGGCCCGCC AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA 
GATGCCAAGA AGCTCCCCTC CCCTGCCCGG GCAGCGGAAG CCGACAAGGA GCCCCAGCGC 
CTGCTGGTGC CTGACATCCA GGAAATCCGT GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC 
CTGCACTTCC TGGAGCCGCA CACGGCCGGC TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG 
CCCTACGCCT ACATGTACAA CCACGACAAG GACGCCGTGG AGCGCTTCGT GCTCAACCTG 
TCCACCGCCC AGGTGGAGTA CAGCGAGGAC CAGCAGGCGA TGCTCAAGAC CCCCAACACG 
TTCGCGGTGT GCACGGAGCA CCGGGGCATC TTGCTGCAGG CCAGCAGTGA CAAGGACATG 
CACGACTGGC TGTACGCCTT CAACCCCCTG CTGGCCGGGA CCATCCGGTC CAAGCTGTCC 
AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462891.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318377.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

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CloneID	OBa51460
Clone ID Related Accession (Same CDS sequence)	XM_015462891.1 , XM_010803929.2
Accession Version	XM_015462891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGGCAG TTTCTTGATT CGGACATGCC TAGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318377.1
CDS	323..5548
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462891.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462891.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGGCAG TTTCTTGATT CGGACATGCC TAGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803929.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802231.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

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CloneID	OBa51460
Clone ID Related Accession (Same CDS sequence)	XM_015462891.1 , XM_010803929.2
Accession Version	XM_010803929.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGGCAG TTTCTTGATT CGGACATGCC TAGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802231.1
CDS	323..5548
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803929.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803929.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG GGCCTTCGTG TACCTCAGCA ACCTGCTGTA CCCCGTGCCG 
CTGGTGCACC GCGTGGCCGT CGTCAGCGAG AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG 
GCCGTGCAGG CCATCTCAGC CGACGAGGAG GCCCCTGACT ACGGCTCCGG CGTCCGCCAG 
TCGGGAACAG CCAAAATCTC CTTTGACGAC CAGCATTTTG AGAAGTTCCA GGCCGAGTCC 
TGCCCCGGGG TGGGCATGTC CCGCTCAGGG ACCTCCCAGG AGGAGCTGCG GATTGTGGAA 
GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC TCAGCCGACG AGGTCAACAA CAACACCTGT 
TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC AGCCCTGAGA AAGCCGCCCT GGATGGGCCC 
CTGGACGCCG CCCTGGACCA CCTCGGCCTG GGCAGCACCT TCACCTTCCG CGTGACCGTC 
CTGCAGGCGT CCAGCATATC CGCTGAATAT GCCGACATCT TCTGCCAGTT CAACTTCATC 
CATCGCCATG ACGAGGCCTT CTCTACGGAG CCCCTGAAGA ACACGGGGAG GGGACCCCCG 
CTCGGCTTCT ACCACGTCCA AAACATTGCG GTGGAGGTGA CCAGGTCCTT CATCGAGTAC 
ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC TTCGGGCACT ACCAGCAGCA CCCGTTCCCG 
CCCCTCTGCA AGGACGTGCT CAGCCCCCTG AGGCCCTCTC GCCGCCACTT CCCACGGGTC 
ATGCCGCTGT CCAAGCCAGT GCCGGCCACC AAGCTCAGCA CGCTGACGCG ACCCTGCCCG 
GGCCCCTGCC ACTGCAAGTA CGACCTGCTG GTCTACTTCG AGATCTGCGA GCTGGAGGCC 
AACGGCGACT ACATCCCCGC AGTGGTGGAT CACCGCGGCG GGATGCCGTG CATGGGGACC 
TTCCTGCTCC ACCAGGGCAT CCAGAGACGG ATCACCGTGA CCCTGCTGCA CGAGACGGGC 
AGCCACATCC GCTGGAAGGA AGTGCGGGAG CTGGTGGTGG GTCGCATCCG AAACACCCCG 
GAAACGGACG AGTCCCTGAT TGACCCTAAT ATCTTGTCGC TCAGCATCCT CTCTTCTGGA 
TACATCTGCC CAGCCCAGGA TGACCGGCAG TTTCTTGATT CGGACATGCC TAGCGTTTCG 
TTTGGAAGTG ACACTAGAAC CTTTTACCAG TTTGAGGCTG CGTGGGACAG CTCCATGCAC 
AACTCCCTCC TGCTGAACCG GGTCACCCCT TACCGAGAGA AGATTTACAT GACCCTCTCT 
GCTTATATCG AGATGGAGAA CTGCACGCAG CCGGCCGTGG TCACCAAAGA CTTCTGCATG 
GTCTTCTACT CGCGCGACGC CAAGCTGCCC GCCTCGCGCT CCATCCGCAA CCTGTTCGGC 
AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC CGCGTGACGG GCGTGTACGA GCTGAGTCTG 
TGCCACGTGG CTGACGCGGG CAGCCCAGGC ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC 
ACATCCGTGG CCTACGTCCG CGGCGAGGAG AACCTGGCGG GCTGGAGGCC GCGTAGTGAC 
AGCCTCATCC TGGACCACCA GTGGGAGCTG GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG 
AAGACCAGGC ACTACCTGTT GCTGCGAGAG AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC 
GAGACGCTGT CCCCCGCCGC CAGTGACGGC TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC 
TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG 
GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC ACCCACTCCT TCAACAGAGA GTACACCCAC 
AGCCACGTCT GCGTCAGCGC CAGCGAGAGC AAGCTCTCTG AGATGTCGGT CACCCTGCTG 
CGGGACCCGT CCATGTCCCC GCTGGGGGCC GCCACGCTCA CTCCCTCCTC TACCTGCCCC 
TCCCTGGTCG AGGGCCGGTA TGGGGCCACC GACCTGAGGA CCCCACAGCC CTGCTCCCGG 
CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC GAGGTAGATG CCAAGAAGCT CCCCTCCCCT 
GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC CAGCGCCTGC TGGTGCCTGA CATCCAGGAA 
ATCCGTGTCA GCCCCATCGT CTCCAAGAAG GGCTACCTGC ACTTCCTGGA GCCGCACACG 
GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG CGGCGGCCCT ACGCCTACAT GTACAACCAC 
GACAAGGACG CCGTGGAGCG CTTCGTGCTC AACCTGTCCA CCGCCCAGGT GGAGTACAGC 
GAGGACCAGC AGGCGATGCT CAAGACCCCC AACACGTTCG CGGTGTGCAC GGAGCACCGG 
GGCATCTTGC TGCAGGCCAG CAGTGACAAG GACATGCACG ACTGGCTGTA CGCCTTCAAC 
CCCCTGCTGG CCGGGACCAT CCGGTCCAAG CTGTCCAGAA GGAGGTCTGC CCAGATGCGG 
GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205055.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205112.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

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CloneID	OBa18293
Clone ID Related Accession (Same CDS sequence)	XM_005205055.3 , XM_015462888.1
Accession Version	XM_005205055.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205055.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGGC AGTTTCTTGA TTCGGACATG 
CCTAGCGTTT CGTTTGGAAG TGACACTAGA ACCTTTTACC AGTTTGAGGC TGCGTGGGAC 
AGCTCCATGC ACAACTCCCT CCTGCTGAAC CGGGTCACCC CTTACCGAGA GAAGATTTAC 
ATGACCCTCT CTGCTTATAT CGAGATGGAG AACTGCACGC AGCCGGCCGT GGTCACCAAA 
GACTTCTGCA TGGTCTTCTA CTCGCGCGAC GCCAAGCTGC CCGCCTCGCG CTCCATCCGC 
AACCTGTTCG GCAGTGGGAG CCTGCGGGCC TCAGAGAGCA ACCGCGTGAC GGGCGTGTAC 
GAGCTGAGTC TGTGCCACGT GGCTGACGCG GGCAGCCCAG GCATGCAGCG GCGGCGCCGG 
CGGGTGCTGG ACACATCCGT GGCCTACGTC CGCGGCGAGG AGAACCTGGC GGGCTGGAGG 
CCGCGTAGTG ACAGCCTCAT CCTGGACCAC CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG 
CAGGAGGTGG AGAAGACCAG GCACTACCTG TTGCTGCGAG AGAAGCTGGA GGCGGCCCAG 
CGGCCCGGCC CCGAGACGCT GTCCCCCGCC GCCAGTGACG GCTCCGAGGC CCACGGCTCC 
TCCAGTGCCT CCTCCCCGCT CACGGCTGAG GCCCGGCCAG CATCCCTGGA GGCGCCCAGC 
GAGCGGCAGC GGGAGCTGGC TGTCAAGTGC TTACGGCTCC TCACCCACTC CTTCAACAGA 
GAGTACACCC ACAGCCACGT CTGCGTCAGC GCCAGCGAGA GCAAGCTCTC TGAGATGTCG 
GTCACCCTGC TGCGGGACCC GTCCATGTCC CCGCTGGGGG CCGCCACGCT CACTCCCTCC 
TCTACCTGCC CCTCCCTGGT CGAGGGCCGG TATGGGGCCA CCGACCTGAG GACCCCACAG 
CCCTGCTCCC GGCCCGCCAG CCCGGAGCCT GAGCCCCTGC CCGAGGTAGA TGCCAAGAAG 
CTCCCCTCCC CTGCCCGGGC AGCGGAAGCC GACAAGGAGC CCCAGCGCCT GCTGGTGCCT 
GACATCCAGG AAATCCGTGT CAGCCCCATC GTCTCCAAGA AGGGCTACCT GCACTTCCTG 
GAGCCGCACA CGGCCGGCTG GGCCAAGCGC TTCGTGGTGG TGCGGCGGCC CTACGCCTAC 
ATGTACAACC ACGACAAGGA CGCCGTGGAG CGCTTCGTGC TCAACCTGTC CACCGCCCAG 
GTGGAGTACA GCGAGGACCA GCAGGCGATG CTCAAGACCC CCAACACGTT CGCGGTGTGC 
ACGGAGCACC GGGGCATCTT GCTGCAGGCC AGCAGTGACA AGGACATGCA CGACTGGCTG 
TACGCCTTCA ACCCCCTGCT GGCCGGGACC ATCCGGTCCA AGCTGTCCAG AAGGAGGTCT 
GCCCAGATGC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205112.1
CDS	323..5740
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205055.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205055.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGGC AGTTTCTTGA TTCGGACATG 
CCTAGCGTTT CGTTTGGAAG TGACACTAGA ACCTTTTACC AGTTTGAGGC TGCGTGGGAC 
AGCTCCATGC ACAACTCCCT CCTGCTGAAC CGGGTCACCC CTTACCGAGA GAAGATTTAC 
ATGACCCTCT CTGCTTATAT CGAGATGGAG AACTGCACGC AGCCGGCCGT GGTCACCAAA 
GACTTCTGCA TGGTCTTCTA CTCGCGCGAC GCCAAGCTGC CCGCCTCGCG CTCCATCCGC 
AACCTGTTCG GCAGTGGGAG CCTGCGGGCC TCAGAGAGCA ACCGCGTGAC GGGCGTGTAC 
GAGCTGAGTC TGTGCCACGT GGCTGACGCG GGCAGCCCAG GCATGCAGCG GCGGCGCCGG 
CGGGTGCTGG ACACATCCGT GGCCTACGTC CGCGGCGAGG AGAACCTGGC GGGCTGGAGG 
CCGCGTAGTG ACAGCCTCAT CCTGGACCAC CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG 
CAGGAGGTGG AGAAGACCAG GCACTACCTG TTGCTGCGAG AGAAGCTGGA GGCGGCCCAG 
CGGCCCGGCC CCGAGACGCT GTCCCCCGCC GCCAGTGACG GCTCCGAGGC CCACGGCTCC 
TCCAGTGCCT CCTCCCCGCT CACGGCTGAG GCCCGGCCAG CATCCCTGGA GGCGCCCAGC 
GAGCGGCAGC GGGAGCTGGC TGTCAAGTGC TTACGGCTCC TCACCCACTC CTTCAACAGA 
GAGTACACCC ACAGCCACGT CTGCGTCAGC GCCAGCGAGA GCAAGCTCTC TGAGATGTCG 
GTCACCCTGC TGCGGGACCC GTCCATGTCC CCGCTGGGGG CCGCCACGCT CACTCCCTCC 
TCTACCTGCC CCTCCCTGGT CGAGGGCCGG TATGGGGCCA CCGACCTGAG GACCCCACAG 
CCCTGCTCCC GGCCCGCCAG CCCGGAGCCT GAGCCCCTGC CCGAGGTAGA TGCCAAGAAG 
CTCCCCTCCC CTGCCCGGGC AGCGGAAGCC GACAAGGAGC CCCAGCGCCT GCTGGTGCCT 
GACATCCAGG AAATCCGTGT CAGCCCCATC GTCTCCAAGA AGGGCTACCT GCACTTCCTG 
GAGCCGCACA CGGCCGGCTG GGCCAAGCGC TTCGTGGTGG TGCGGCGGCC CTACGCCTAC 
ATGTACAACC ACGACAAGGA CGCCGTGGAG CGCTTCGTGC TCAACCTGTC CACCGCCCAG 
GTGGAGTACA GCGAGGACCA GCAGGCGATG CTCAAGACCC CCAACACGTT CGCGGTGTGC 
ACGGAGCACC GGGGCATCTT GCTGCAGGCC AGCAGTGACA AGGACATGCA CGACTGGCTG 
TACGCCTTCA ACCCCCTGCT GGCCGGGACC ATCCGGTCCA AGCTGTCCAG AAGGAGGTCT 
GCCCAGATGC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462888.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318374.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

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CloneID	OBa18293
Clone ID Related Accession (Same CDS sequence)	XM_005205055.3 , XM_015462888.1
Accession Version	XM_015462888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGGC AGTTTCTTGA TTCGGACATG 
CCTAGCGTTT CGTTTGGAAG TGACACTAGA ACCTTTTACC AGTTTGAGGC TGCGTGGGAC 
AGCTCCATGC ACAACTCCCT CCTGCTGAAC CGGGTCACCC CTTACCGAGA GAAGATTTAC 
ATGACCCTCT CTGCTTATAT CGAGATGGAG AACTGCACGC AGCCGGCCGT GGTCACCAAA 
GACTTCTGCA TGGTCTTCTA CTCGCGCGAC GCCAAGCTGC CCGCCTCGCG CTCCATCCGC 
AACCTGTTCG GCAGTGGGAG CCTGCGGGCC TCAGAGAGCA ACCGCGTGAC GGGCGTGTAC 
GAGCTGAGTC TGTGCCACGT GGCTGACGCG GGCAGCCCAG GCATGCAGCG GCGGCGCCGG 
CGGGTGCTGG ACACATCCGT GGCCTACGTC CGCGGCGAGG AGAACCTGGC GGGCTGGAGG 
CCGCGTAGTG ACAGCCTCAT CCTGGACCAC CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG 
CAGGAGGTGG AGAAGACCAG GCACTACCTG TTGCTGCGAG AGAAGCTGGA GGCGGCCCAG 
CGGCCCGGCC CCGAGACGCT GTCCCCCGCC GCCAGTGACG GCTCCGAGGC CCACGGCTCC 
TCCAGTGCCT CCTCCCCGCT CACGGCTGAG GCCCGGCCAG CATCCCTGGA GGCGCCCAGC 
GAGCGGCAGC GGGAGCTGGC TGTCAAGTGC TTACGGCTCC TCACCCACTC CTTCAACAGA 
GAGTACACCC ACAGCCACGT CTGCGTCAGC GCCAGCGAGA GCAAGCTCTC TGAGATGTCG 
GTCACCCTGC TGCGGGACCC GTCCATGTCC CCGCTGGGGG CCGCCACGCT CACTCCCTCC 
TCTACCTGCC CCTCCCTGGT CGAGGGCCGG TATGGGGCCA CCGACCTGAG GACCCCACAG 
CCCTGCTCCC GGCCCGCCAG CCCGGAGCCT GAGCCCCTGC CCGAGGTAGA TGCCAAGAAG 
CTCCCCTCCC CTGCCCGGGC AGCGGAAGCC GACAAGGAGC CCCAGCGCCT GCTGGTGCCT 
GACATCCAGG AAATCCGTGT CAGCCCCATC GTCTCCAAGA AGGGCTACCT GCACTTCCTG 
GAGCCGCACA CGGCCGGCTG GGCCAAGCGC TTCGTGGTGG TGCGGCGGCC CTACGCCTAC 
ATGTACAACC ACGACAAGGA CGCCGTGGAG CGCTTCGTGC TCAACCTGTC CACCGCCCAG 
GTGGAGTACA GCGAGGACCA GCAGGCGATG CTCAAGACCC CCAACACGTT CGCGGTGTGC 
ACGGAGCACC GGGGCATCTT GCTGCAGGCC AGCAGTGACA AGGACATGCA CGACTGGCTG 
TACGCCTTCA ACCCCCTGCT GGCCGGGACC ATCCGGTCCA AGCTGTCCAG AAGGAGGTCT 
GCCCAGATGC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318374.1
CDS	323..5740
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462888.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462888.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGGC AGTTTCTTGA TTCGGACATG 
CCTAGCGTTT CGTTTGGAAG TGACACTAGA ACCTTTTACC AGTTTGAGGC TGCGTGGGAC 
AGCTCCATGC ACAACTCCCT CCTGCTGAAC CGGGTCACCC CTTACCGAGA GAAGATTTAC 
ATGACCCTCT CTGCTTATAT CGAGATGGAG AACTGCACGC AGCCGGCCGT GGTCACCAAA 
GACTTCTGCA TGGTCTTCTA CTCGCGCGAC GCCAAGCTGC CCGCCTCGCG CTCCATCCGC 
AACCTGTTCG GCAGTGGGAG CCTGCGGGCC TCAGAGAGCA ACCGCGTGAC GGGCGTGTAC 
GAGCTGAGTC TGTGCCACGT GGCTGACGCG GGCAGCCCAG GCATGCAGCG GCGGCGCCGG 
CGGGTGCTGG ACACATCCGT GGCCTACGTC CGCGGCGAGG AGAACCTGGC GGGCTGGAGG 
CCGCGTAGTG ACAGCCTCAT CCTGGACCAC CAGTGGGAGC TGGAGAAGCT GAGTCTCCTG 
CAGGAGGTGG AGAAGACCAG GCACTACCTG TTGCTGCGAG AGAAGCTGGA GGCGGCCCAG 
CGGCCCGGCC CCGAGACGCT GTCCCCCGCC GCCAGTGACG GCTCCGAGGC CCACGGCTCC 
TCCAGTGCCT CCTCCCCGCT CACGGCTGAG GCCCGGCCAG CATCCCTGGA GGCGCCCAGC 
GAGCGGCAGC GGGAGCTGGC TGTCAAGTGC TTACGGCTCC TCACCCACTC CTTCAACAGA 
GAGTACACCC ACAGCCACGT CTGCGTCAGC GCCAGCGAGA GCAAGCTCTC TGAGATGTCG 
GTCACCCTGC TGCGGGACCC GTCCATGTCC CCGCTGGGGG CCGCCACGCT CACTCCCTCC 
TCTACCTGCC CCTCCCTGGT CGAGGGCCGG TATGGGGCCA CCGACCTGAG GACCCCACAG 
CCCTGCTCCC GGCCCGCCAG CCCGGAGCCT GAGCCCCTGC CCGAGGTAGA TGCCAAGAAG 
CTCCCCTCCC CTGCCCGGGC AGCGGAAGCC GACAAGGAGC CCCAGCGCCT GCTGGTGCCT 
GACATCCAGG AAATCCGTGT CAGCCCCATC GTCTCCAAGA AGGGCTACCT GCACTTCCTG 
GAGCCGCACA CGGCCGGCTG GGCCAAGCGC TTCGTGGTGG TGCGGCGGCC CTACGCCTAC 
ATGTACAACC ACGACAAGGA CGCCGTGGAG CGCTTCGTGC TCAACCTGTC CACCGCCCAG 
GTGGAGTACA GCGAGGACCA GCAGGCGATG CTCAAGACCC CCAACACGTT CGCGGTGTGC 
ACGGAGCACC GGGGCATCTT GCTGCAGGCC AGCAGTGACA AGGACATGCA CGACTGGCTG 
TACGCCTTCA ACCCCCTGCT GGCCGGGACC ATCCGGTCCA AGCTGTCCAG AAGGAGGTCT 
GCCCAGATGC GGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462895.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318381.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X14, mRNA.

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CloneID	OBa18295
Clone ID Related Accession (Same CDS sequence)	XM_015462895.1
Accession Version	XM_015462895.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGCG TTTCGTTTGG AAGTGACACT 
AGAACCTTTT ACCAGTTTGA GGCTGCGTGG GACAGCTCCA TGCACAACTC CCTCCTGCTG 
AACCGGGTCA CCCCTTACCG AGAGAAGATT TACATGACCC TCTCTGCTTA TATCGAGATG 
GAGAACTGCA CGCAGCCGGC CGTGGTCACC AAAGACTTCT GCATGGTCTT CTACTCGCGC 
GACGCCAAGC TGCCCGCCTC GCGCTCCATC CGCAACCTGT TCGGCAGTGG GAGCCTGCGG 
GCCTCAGAGA GCAACCGCGT GACGGGCGTG TACGAGCTGA GTCTGTGCCA CGTGGCTGAC 
GCGGGCAGCC CAGGCATGCA GCGGCGGCGC CGGCGGGTGC TGGACACATC CGTGGCCTAC 
GTCCGCGGCG AGGAGAACCT GGCGGGCTGG AGGCCGCGTA GTGACAGCCT CATCCTGGAC 
CACCAGTGGG AGCTGGAGAA GCTGAGTCTC CTGCAGGAGG TGGAGAAGAC CAGGCACTAC 
CTGTTGCTGC GAGAGAAGCT GGAGGCGGCC CAGCGGCCCG GCCCCGAGAC GCTGTCCCCC 
GCCGCCAGTG ACGGCTCCGA GGCCCACGGC TCCTCCAGTG CCTCCTCCCC GCTCACGGCT 
GAGGCCCGGC CAGCATCCCT GGAGGCGCCC AGCGAGCGGC AGCGGGAGCT GGCTGTCAAG 
TGCTTACGGC TCCTCACCCA CTCCTTCAAC AGAGAGTACA CCCACAGCCA CGTCTGCGTC 
AGCGCCAGCG AGAGCAAGCT CTCTGAGATG TCGGTCACCC TGCTGCGGGA CCCGTCCATG 
TCCCCGCTGG GGGCCGCCAC GCTCACTCCC TCCTCTACCT GCCCCTCCCT GGTCGAGGGC 
CGGTATGGGG CCACCGACCT GAGGACCCCA CAGCCCTGCT CCCGGCCCGC CAGCCCGGAG 
CCTGAGCCCC TGCCCGAGGT AGATGCCAAG AAGCTCCCCT CCCCTGCCCG GGCAGCGGAA 
GCCGACAAGG AGCCCCAGCG CCTGCTGGTG CCTGACATCC AGGAAATCCG TGTCAGCCCC 
ATCGTCTCCA AGAAGGGCTA CCTGCACTTC CTGGAGCCGC ACACGGCCGG CTGGGCCAAG 
CGCTTCGTGG TGGTGCGGCG GCCCTACGCC TACATGTACA ACCACGACAA GGACGCCGTG 
GAGCGCTTCG TGCTCAACCT GTCCACCGCC CAGGTGGAGT ACAGCGAGGA CCAGCAGGCG 
ATGCTCAAGA CCCCCAACAC GTTCGCGGTG TGCACGGAGC ACCGGGGCAT CTTGCTGCAG 
GCCAGCAGTG ACAAGGACAT GCACGACTGG CTGTACGCCT TCAACCCCCT GCTGGCCGGG 
ACCATCCGGT CCAAGCTGTC CAGAAGGAGG TCTGCCCAGA TGCGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318381.1
CDS	13..5403
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462895.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462895.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGCG TTTCGTTTGG AAGTGACACT 
AGAACCTTTT ACCAGTTTGA GGCTGCGTGG GACAGCTCCA TGCACAACTC CCTCCTGCTG 
AACCGGGTCA CCCCTTACCG AGAGAAGATT TACATGACCC TCTCTGCTTA TATCGAGATG 
GAGAACTGCA CGCAGCCGGC CGTGGTCACC AAAGACTTCT GCATGGTCTT CTACTCGCGC 
GACGCCAAGC TGCCCGCCTC GCGCTCCATC CGCAACCTGT TCGGCAGTGG GAGCCTGCGG 
GCCTCAGAGA GCAACCGCGT GACGGGCGTG TACGAGCTGA GTCTGTGCCA CGTGGCTGAC 
GCGGGCAGCC CAGGCATGCA GCGGCGGCGC CGGCGGGTGC TGGACACATC CGTGGCCTAC 
GTCCGCGGCG AGGAGAACCT GGCGGGCTGG AGGCCGCGTA GTGACAGCCT CATCCTGGAC 
CACCAGTGGG AGCTGGAGAA GCTGAGTCTC CTGCAGGAGG TGGAGAAGAC CAGGCACTAC 
CTGTTGCTGC GAGAGAAGCT GGAGGCGGCC CAGCGGCCCG GCCCCGAGAC GCTGTCCCCC 
GCCGCCAGTG ACGGCTCCGA GGCCCACGGC TCCTCCAGTG CCTCCTCCCC GCTCACGGCT 
GAGGCCCGGC CAGCATCCCT GGAGGCGCCC AGCGAGCGGC AGCGGGAGCT GGCTGTCAAG 
TGCTTACGGC TCCTCACCCA CTCCTTCAAC AGAGAGTACA CCCACAGCCA CGTCTGCGTC 
AGCGCCAGCG AGAGCAAGCT CTCTGAGATG TCGGTCACCC TGCTGCGGGA CCCGTCCATG 
TCCCCGCTGG GGGCCGCCAC GCTCACTCCC TCCTCTACCT GCCCCTCCCT GGTCGAGGGC 
CGGTATGGGG CCACCGACCT GAGGACCCCA CAGCCCTGCT CCCGGCCCGC CAGCCCGGAG 
CCTGAGCCCC TGCCCGAGGT AGATGCCAAG AAGCTCCCCT CCCCTGCCCG GGCAGCGGAA 
GCCGACAAGG AGCCCCAGCG CCTGCTGGTG CCTGACATCC AGGAAATCCG TGTCAGCCCC 
ATCGTCTCCA AGAAGGGCTA CCTGCACTTC CTGGAGCCGC ACACGGCCGG CTGGGCCAAG 
CGCTTCGTGG TGGTGCGGCG GCCCTACGCC TACATGTACA ACCACGACAA GGACGCCGTG 
GAGCGCTTCG TGCTCAACCT GTCCACCGCC CAGGTGGAGT ACAGCGAGGA CCAGCAGGCG 
ATGCTCAAGA CCCCCAACAC GTTCGCGGTG TGCACGGAGC ACCGGGGCAT CTTGCTGCAG 
GCCAGCAGTG ACAAGGACAT GCACGACTGG CTGTACGCCT TCAACCCCCT GCTGGCCGGG 
ACCATCCGGT CCAAGCTGTC CAGAAGGAGG TCTGCCCAGA TGCGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_005205058.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005205115.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

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CloneID	OBa18296
Clone ID Related Accession (Same CDS sequence)	XM_005205058.3 , XM_015462889.1
Accession Version	XM_005205058.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005205058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGC AGGACGACGT CTTTCCGGAG 
CACGTGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTGTACCT CAGCAACCTG CTGTACCCCG TGCCGCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG GGCTTCCTGC GCGTGGCCGT GCAGGCCATC 
TCAGCCGACG AGGAGGCCCC TGACTACGGC TCCGGCGTCC GCCAGTCGGG AACAGCCAAA 
ATCTCCTTTG ACGACCAGCA TTTTGAGAAG TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC 
ATGTCCCGCT CAGGGACCTC CCAGGAGGAG CTGCGGATTG TGGAAGGCCA GGGCCAGGCC 
GCAGACTCCG GGCCCTCAGC CGACGAGGTC AACAACAACA CCTGTTCAGC GGTGACCCCG 
GAAGGCCTCC TGGACAGCCC TGAGAAAGCC GCCCTGGATG GGCCCCTGGA CGCCGCCCTG 
GACCACCTCG GCCTGGGCAG CACCTTCACC TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC 
ATATCCGCTG AATATGCCGA CATCTTCTGC CAGTTCAACT TCATCCATCG CCATGACGAG 
GCCTTCTCTA CGGAGCCCCT GAAGAACACG GGGAGGGGAC CCCCGCTCGG CTTCTACCAC 
GTCCAAAACA TTGCGGTGGA GGTGACCAGG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
CTCGTGTTCG AGGTCTTCGG GCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAC 
GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC CACTTCCCAC GGGTCATGCC GCTGTCCAAG 
CCAGTGCCGG CCACCAAGCT CAGCACGCTG ACGCGACCCT GCCCGGGCCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTCGAGATC TGCGAGCTGG AGGCCAACGG CGACTACATC 
CCCGCAGTGG TGGATCACCG CGGCGGGATG CCGTGCATGG GGACCTTCCT GCTCCACCAG 
GGCATCCAGA GACGGATCAC CGTGACCCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC ATCCGAAACA CCCCGGAAAC GGACGAGTCC 
CTGATTGACC CTAATATCTT GTCGCTCAGC ATCCTCTCTT CTGGATACAT CTGCCCAGCC 
CAGGATGACC GGCAGTTTCT TGATTCGGAC ATGCCTAGCG TTTCGTTTGG AAGTGACACT 
AGAACCTTTT ACCAGTTTGA GGCTGCGTGG GACAGCTCCA TGCACAACTC CCTCCTGCTG 
AACCGGGTCA CCCCTTACCG AGAGAAGATT TACATGACCC TCTCTGCTTA TATCGAGATG 
GAGAACTGCA CGCAGCCGGC CGTGGTCACC AAAGACTTCT GCATGGTCTT CTACTCGCGC 
GACGCCAAGC TGCCCGCCTC GCGCTCCATC CGCAACCTGT TCGGCAGTGG GAGCCTGCGG 
GCCTCAGAGA GCAACCGCGT GACGGGCGTG TACGAGCTGA GTCTGTGCCA CGTGGCTGAC 
GCGGGCAGCC CAGGCATGCA GCGGCGGCGC CGGCGGGTGC TGGACACATC CGTGGCCTAC 
GTCCGCGGCG AGGAGAACCT GGCGGGCTGG AGGCCGCGTA GTGACAGCCT CATCCTGGAC 
CACCAGTGGG AGCTGGAGAA GCTGAGTCTC CTGCAGGAGG TGGAGAAGAC CAGGCACTAC 
CTGTTGCTGC GAGAGAAGCT GGAGGCGGCC CAGCGGCCCG GCCCCGAGAC GCTGTCCCCC 
GCCGCCAGTG ACGGCTCCGA GGCCCACGGC TCCTCCAGTG CCTCCTCCCC GCTCACGGCT 
GAGGCCCGGC CAGCATCCCT GGAGGCGCCC AGCGAGCGGC AGCGGGAGCT GGCTGTCAAG 
TGCTTACGGC TCCTCACCCA CTCCTTCAAC AGAGAGTACA CCCACAGCCA CGTCTGCGTC 
AGCGCCAGCG AGAGCAAGCT CTCTGAGATG TCGGTCACCC TGCTGCGGGA CCCGTCCATG 
TCCCCGCTGG GGGCCGCCAC GCTCACTCCC TCCTCTACCT GCCCCTCCCT GGTCGAGGGC 
CGGTATGGGG CCACCGACCT GAGGACCCCA CAGCCCTGCT CCCGGCCCGC CAGCCCGGAG 
CCTGAGCCCC TGCCCGAGGT AGATGCCAAG AAGCTCCCCT CCCCTGCCCG GGCAGCGGAA 
GCCGACAAGG AGCCCCAGCG CCTGCTGGTG CCTGACATCC AGGAAATCCG TGTCAGCCCC 
ATCGTCTCCA AGAAGGGCTA CCTGCACTTC CTGGAGCCGC ACACGGCCGG CTGGGCCAAG 
CGCTTCGTGG TGGTGCGGCG GCCCTACGCC TACATGTACA ACCACGACAA GGACGCCGTG 
GAGCGCTTCG TGCTCAACCT GTCCACCGCC CAGGTGGAGT ACAGCGAGGA CCAGCAGGCG 
ATGCTCAAGA CCCCCAACAC GTTCGCGGTG TGCACGGAGC ACCGGGGCAT CTTGCTGCAG 
GCCAGCAGTG ACAAGGACAT GCACGACTGG CTGTACGCCT TCAACCCCCT GCTGGCCGGG 
ACCATCCGGT CCAAGCTGTC CAGAAGGAGG TCTGCCCAGA TGCGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005205115.1
CDS	323..5713
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_005205058.3
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005205058.3

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGC AGGACGACGT CTTTCCGGAG 
CACGTGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTGTACCT CAGCAACCTG CTGTACCCCG TGCCGCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG GGCTTCCTGC GCGTGGCCGT GCAGGCCATC 
TCAGCCGACG AGGAGGCCCC TGACTACGGC TCCGGCGTCC GCCAGTCGGG AACAGCCAAA 
ATCTCCTTTG ACGACCAGCA TTTTGAGAAG TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC 
ATGTCCCGCT CAGGGACCTC CCAGGAGGAG CTGCGGATTG TGGAAGGCCA GGGCCAGGCC 
GCAGACTCCG GGCCCTCAGC CGACGAGGTC AACAACAACA CCTGTTCAGC GGTGACCCCG 
GAAGGCCTCC TGGACAGCCC TGAGAAAGCC GCCCTGGATG GGCCCCTGGA CGCCGCCCTG 
GACCACCTCG GCCTGGGCAG CACCTTCACC TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC 
ATATCCGCTG AATATGCCGA CATCTTCTGC CAGTTCAACT TCATCCATCG CCATGACGAG 
GCCTTCTCTA CGGAGCCCCT GAAGAACACG GGGAGGGGAC CCCCGCTCGG CTTCTACCAC 
GTCCAAAACA TTGCGGTGGA GGTGACCAGG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
CTCGTGTTCG AGGTCTTCGG GCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAC 
GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC CACTTCCCAC GGGTCATGCC GCTGTCCAAG 
CCAGTGCCGG CCACCAAGCT CAGCACGCTG ACGCGACCCT GCCCGGGCCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTCGAGATC TGCGAGCTGG AGGCCAACGG CGACTACATC 
CCCGCAGTGG TGGATCACCG CGGCGGGATG CCGTGCATGG GGACCTTCCT GCTCCACCAG 
GGCATCCAGA GACGGATCAC CGTGACCCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC ATCCGAAACA CCCCGGAAAC GGACGAGTCC 
CTGATTGACC CTAATATCTT GTCGCTCAGC ATCCTCTCTT CTGGATACAT CTGCCCAGCC 
CAGGATGACC GGCAGTTTCT TGATTCGGAC ATGCCTAGCG TTTCGTTTGG AAGTGACACT 
AGAACCTTTT ACCAGTTTGA GGCTGCGTGG GACAGCTCCA TGCACAACTC CCTCCTGCTG 
AACCGGGTCA CCCCTTACCG AGAGAAGATT TACATGACCC TCTCTGCTTA TATCGAGATG 
GAGAACTGCA CGCAGCCGGC CGTGGTCACC AAAGACTTCT GCATGGTCTT CTACTCGCGC 
GACGCCAAGC TGCCCGCCTC GCGCTCCATC CGCAACCTGT TCGGCAGTGG GAGCCTGCGG 
GCCTCAGAGA GCAACCGCGT GACGGGCGTG TACGAGCTGA GTCTGTGCCA CGTGGCTGAC 
GCGGGCAGCC CAGGCATGCA GCGGCGGCGC CGGCGGGTGC TGGACACATC CGTGGCCTAC 
GTCCGCGGCG AGGAGAACCT GGCGGGCTGG AGGCCGCGTA GTGACAGCCT CATCCTGGAC 
CACCAGTGGG AGCTGGAGAA GCTGAGTCTC CTGCAGGAGG TGGAGAAGAC CAGGCACTAC 
CTGTTGCTGC GAGAGAAGCT GGAGGCGGCC CAGCGGCCCG GCCCCGAGAC GCTGTCCCCC 
GCCGCCAGTG ACGGCTCCGA GGCCCACGGC TCCTCCAGTG CCTCCTCCCC GCTCACGGCT 
GAGGCCCGGC CAGCATCCCT GGAGGCGCCC AGCGAGCGGC AGCGGGAGCT GGCTGTCAAG 
TGCTTACGGC TCCTCACCCA CTCCTTCAAC AGAGAGTACA CCCACAGCCA CGTCTGCGTC 
AGCGCCAGCG AGAGCAAGCT CTCTGAGATG TCGGTCACCC TGCTGCGGGA CCCGTCCATG 
TCCCCGCTGG GGGCCGCCAC GCTCACTCCC TCCTCTACCT GCCCCTCCCT GGTCGAGGGC 
CGGTATGGGG CCACCGACCT GAGGACCCCA CAGCCCTGCT CCCGGCCCGC CAGCCCGGAG 
CCTGAGCCCC TGCCCGAGGT AGATGCCAAG AAGCTCCCCT CCCCTGCCCG GGCAGCGGAA 
GCCGACAAGG AGCCCCAGCG CCTGCTGGTG CCTGACATCC AGGAAATCCG TGTCAGCCCC 
ATCGTCTCCA AGAAGGGCTA CCTGCACTTC CTGGAGCCGC ACACGGCCGG CTGGGCCAAG 
CGCTTCGTGG TGGTGCGGCG GCCCTACGCC TACATGTACA ACCACGACAA GGACGCCGTG 
GAGCGCTTCG TGCTCAACCT GTCCACCGCC CAGGTGGAGT ACAGCGAGGA CCAGCAGGCG 
ATGCTCAAGA CCCCCAACAC GTTCGCGGTG TGCACGGAGC ACCGGGGCAT CTTGCTGCAG 
GCCAGCAGTG ACAAGGACAT GCACGACTGG CTGTACGCCT TCAACCCCCT GCTGGCCGGG 
ACCATCCGGT CCAAGCTGTC CAGAAGGAGG TCTGCCCAGA TGCGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462889.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318375.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

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CloneID	OBa18296
Clone ID Related Accession (Same CDS sequence)	XM_005205058.3 , XM_015462889.1
Accession Version	XM_015462889.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGC AGGACGACGT CTTTCCGGAG 
CACGTGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTGTACCT CAGCAACCTG CTGTACCCCG TGCCGCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG GGCTTCCTGC GCGTGGCCGT GCAGGCCATC 
TCAGCCGACG AGGAGGCCCC TGACTACGGC TCCGGCGTCC GCCAGTCGGG AACAGCCAAA 
ATCTCCTTTG ACGACCAGCA TTTTGAGAAG TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC 
ATGTCCCGCT CAGGGACCTC CCAGGAGGAG CTGCGGATTG TGGAAGGCCA GGGCCAGGCC 
GCAGACTCCG GGCCCTCAGC CGACGAGGTC AACAACAACA CCTGTTCAGC GGTGACCCCG 
GAAGGCCTCC TGGACAGCCC TGAGAAAGCC GCCCTGGATG GGCCCCTGGA CGCCGCCCTG 
GACCACCTCG GCCTGGGCAG CACCTTCACC TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC 
ATATCCGCTG AATATGCCGA CATCTTCTGC CAGTTCAACT TCATCCATCG CCATGACGAG 
GCCTTCTCTA CGGAGCCCCT GAAGAACACG GGGAGGGGAC CCCCGCTCGG CTTCTACCAC 
GTCCAAAACA TTGCGGTGGA GGTGACCAGG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
CTCGTGTTCG AGGTCTTCGG GCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAC 
GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC CACTTCCCAC GGGTCATGCC GCTGTCCAAG 
CCAGTGCCGG CCACCAAGCT CAGCACGCTG ACGCGACCCT GCCCGGGCCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTCGAGATC TGCGAGCTGG AGGCCAACGG CGACTACATC 
CCCGCAGTGG TGGATCACCG CGGCGGGATG CCGTGCATGG GGACCTTCCT GCTCCACCAG 
GGCATCCAGA GACGGATCAC CGTGACCCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC ATCCGAAACA CCCCGGAAAC GGACGAGTCC 
CTGATTGACC CTAATATCTT GTCGCTCAGC ATCCTCTCTT CTGGATACAT CTGCCCAGCC 
CAGGATGACC GGCAGTTTCT TGATTCGGAC ATGCCTAGCG TTTCGTTTGG AAGTGACACT 
AGAACCTTTT ACCAGTTTGA GGCTGCGTGG GACAGCTCCA TGCACAACTC CCTCCTGCTG 
AACCGGGTCA CCCCTTACCG AGAGAAGATT TACATGACCC TCTCTGCTTA TATCGAGATG 
GAGAACTGCA CGCAGCCGGC CGTGGTCACC AAAGACTTCT GCATGGTCTT CTACTCGCGC 
GACGCCAAGC TGCCCGCCTC GCGCTCCATC CGCAACCTGT TCGGCAGTGG GAGCCTGCGG 
GCCTCAGAGA GCAACCGCGT GACGGGCGTG TACGAGCTGA GTCTGTGCCA CGTGGCTGAC 
GCGGGCAGCC CAGGCATGCA GCGGCGGCGC CGGCGGGTGC TGGACACATC CGTGGCCTAC 
GTCCGCGGCG AGGAGAACCT GGCGGGCTGG AGGCCGCGTA GTGACAGCCT CATCCTGGAC 
CACCAGTGGG AGCTGGAGAA GCTGAGTCTC CTGCAGGAGG TGGAGAAGAC CAGGCACTAC 
CTGTTGCTGC GAGAGAAGCT GGAGGCGGCC CAGCGGCCCG GCCCCGAGAC GCTGTCCCCC 
GCCGCCAGTG ACGGCTCCGA GGCCCACGGC TCCTCCAGTG CCTCCTCCCC GCTCACGGCT 
GAGGCCCGGC CAGCATCCCT GGAGGCGCCC AGCGAGCGGC AGCGGGAGCT GGCTGTCAAG 
TGCTTACGGC TCCTCACCCA CTCCTTCAAC AGAGAGTACA CCCACAGCCA CGTCTGCGTC 
AGCGCCAGCG AGAGCAAGCT CTCTGAGATG TCGGTCACCC TGCTGCGGGA CCCGTCCATG 
TCCCCGCTGG GGGCCGCCAC GCTCACTCCC TCCTCTACCT GCCCCTCCCT GGTCGAGGGC 
CGGTATGGGG CCACCGACCT GAGGACCCCA CAGCCCTGCT CCCGGCCCGC CAGCCCGGAG 
CCTGAGCCCC TGCCCGAGGT AGATGCCAAG AAGCTCCCCT CCCCTGCCCG GGCAGCGGAA 
GCCGACAAGG AGCCCCAGCG CCTGCTGGTG CCTGACATCC AGGAAATCCG TGTCAGCCCC 
ATCGTCTCCA AGAAGGGCTA CCTGCACTTC CTGGAGCCGC ACACGGCCGG CTGGGCCAAG 
CGCTTCGTGG TGGTGCGGCG GCCCTACGCC TACATGTACA ACCACGACAA GGACGCCGTG 
GAGCGCTTCG TGCTCAACCT GTCCACCGCC CAGGTGGAGT ACAGCGAGGA CCAGCAGGCG 
ATGCTCAAGA CCCCCAACAC GTTCGCGGTG TGCACGGAGC ACCGGGGCAT CTTGCTGCAG 
GCCAGCAGTG ACAAGGACAT GCACGACTGG CTGTACGCCT TCAACCCCCT GCTGGCCGGG 
ACCATCCGGT CCAAGCTGTC CAGAAGGAGG TCTGCCCAGA TGCGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318375.1
CDS	323..5713
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462889.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462889.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGC AGGACGACGT CTTTCCGGAG 
CACGTGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTGTACCT CAGCAACCTG CTGTACCCCG TGCCGCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG GGCTTCCTGC GCGTGGCCGT GCAGGCCATC 
TCAGCCGACG AGGAGGCCCC TGACTACGGC TCCGGCGTCC GCCAGTCGGG AACAGCCAAA 
ATCTCCTTTG ACGACCAGCA TTTTGAGAAG TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC 
ATGTCCCGCT CAGGGACCTC CCAGGAGGAG CTGCGGATTG TGGAAGGCCA GGGCCAGGCC 
GCAGACTCCG GGCCCTCAGC CGACGAGGTC AACAACAACA CCTGTTCAGC GGTGACCCCG 
GAAGGCCTCC TGGACAGCCC TGAGAAAGCC GCCCTGGATG GGCCCCTGGA CGCCGCCCTG 
GACCACCTCG GCCTGGGCAG CACCTTCACC TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC 
ATATCCGCTG AATATGCCGA CATCTTCTGC CAGTTCAACT TCATCCATCG CCATGACGAG 
GCCTTCTCTA CGGAGCCCCT GAAGAACACG GGGAGGGGAC CCCCGCTCGG CTTCTACCAC 
GTCCAAAACA TTGCGGTGGA GGTGACCAGG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
CTCGTGTTCG AGGTCTTCGG GCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAC 
GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC CACTTCCCAC GGGTCATGCC GCTGTCCAAG 
CCAGTGCCGG CCACCAAGCT CAGCACGCTG ACGCGACCCT GCCCGGGCCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTCGAGATC TGCGAGCTGG AGGCCAACGG CGACTACATC 
CCCGCAGTGG TGGATCACCG CGGCGGGATG CCGTGCATGG GGACCTTCCT GCTCCACCAG 
GGCATCCAGA GACGGATCAC CGTGACCCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC ATCCGAAACA CCCCGGAAAC GGACGAGTCC 
CTGATTGACC CTAATATCTT GTCGCTCAGC ATCCTCTCTT CTGGATACAT CTGCCCAGCC 
CAGGATGACC GGCAGTTTCT TGATTCGGAC ATGCCTAGCG TTTCGTTTGG AAGTGACACT 
AGAACCTTTT ACCAGTTTGA GGCTGCGTGG GACAGCTCCA TGCACAACTC CCTCCTGCTG 
AACCGGGTCA CCCCTTACCG AGAGAAGATT TACATGACCC TCTCTGCTTA TATCGAGATG 
GAGAACTGCA CGCAGCCGGC CGTGGTCACC AAAGACTTCT GCATGGTCTT CTACTCGCGC 
GACGCCAAGC TGCCCGCCTC GCGCTCCATC CGCAACCTGT TCGGCAGTGG GAGCCTGCGG 
GCCTCAGAGA GCAACCGCGT GACGGGCGTG TACGAGCTGA GTCTGTGCCA CGTGGCTGAC 
GCGGGCAGCC CAGGCATGCA GCGGCGGCGC CGGCGGGTGC TGGACACATC CGTGGCCTAC 
GTCCGCGGCG AGGAGAACCT GGCGGGCTGG AGGCCGCGTA GTGACAGCCT CATCCTGGAC 
CACCAGTGGG AGCTGGAGAA GCTGAGTCTC CTGCAGGAGG TGGAGAAGAC CAGGCACTAC 
CTGTTGCTGC GAGAGAAGCT GGAGGCGGCC CAGCGGCCCG GCCCCGAGAC GCTGTCCCCC 
GCCGCCAGTG ACGGCTCCGA GGCCCACGGC TCCTCCAGTG CCTCCTCCCC GCTCACGGCT 
GAGGCCCGGC CAGCATCCCT GGAGGCGCCC AGCGAGCGGC AGCGGGAGCT GGCTGTCAAG 
TGCTTACGGC TCCTCACCCA CTCCTTCAAC AGAGAGTACA CCCACAGCCA CGTCTGCGTC 
AGCGCCAGCG AGAGCAAGCT CTCTGAGATG TCGGTCACCC TGCTGCGGGA CCCGTCCATG 
TCCCCGCTGG GGGCCGCCAC GCTCACTCCC TCCTCTACCT GCCCCTCCCT GGTCGAGGGC 
CGGTATGGGG CCACCGACCT GAGGACCCCA CAGCCCTGCT CCCGGCCCGC CAGCCCGGAG 
CCTGAGCCCC TGCCCGAGGT AGATGCCAAG AAGCTCCCCT CCCCTGCCCG GGCAGCGGAA 
GCCGACAAGG AGCCCCAGCG CCTGCTGGTG CCTGACATCC AGGAAATCCG TGTCAGCCCC 
ATCGTCTCCA AGAAGGGCTA CCTGCACTTC CTGGAGCCGC ACACGGCCGG CTGGGCCAAG 
CGCTTCGTGG TGGTGCGGCG GCCCTACGCC TACATGTACA ACCACGACAA GGACGCCGTG 
GAGCGCTTCG TGCTCAACCT GTCCACCGCC CAGGTGGAGT ACAGCGAGGA CCAGCAGGCG 
ATGCTCAAGA CCCCCAACAC GTTCGCGGTG TGCACGGAGC ACCGGGGCAT CTTGCTGCAG 
GCCAGCAGTG ACAAGGACAT GCACGACTGG CTGTACGCCT TCAACCCCCT GCTGGCCGGG 
ACCATCCGGT CCAAGCTGTC CAGAAGGAGG TCTGCCCAGA TGCGGGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462890.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318376.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

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CloneID	OBa18297
Clone ID Related Accession (Same CDS sequence)	XM_015462890.1 , XM_002686593.5
Accession Version	XM_015462890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGAA CCTTTTACCA GTTTGAGGCT 
GCGTGGGACA GCTCCATGCA CAACTCCCTC CTGCTGAACC GGGTCACCCC TTACCGAGAG 
AAGATTTACA TGACCCTCTC TGCTTATATC GAGATGGAGA ACTGCACGCA GCCGGCCGTG 
GTCACCAAAG ACTTCTGCAT GGTCTTCTAC TCGCGCGACG CCAAGCTGCC CGCCTCGCGC 
TCCATCCGCA ACCTGTTCGG CAGTGGGAGC CTGCGGGCCT CAGAGAGCAA CCGCGTGACG 
GGCGTGTACG AGCTGAGTCT GTGCCACGTG GCTGACGCGG GCAGCCCAGG CATGCAGCGG 
CGGCGCCGGC GGGTGCTGGA CACATCCGTG GCCTACGTCC GCGGCGAGGA GAACCTGGCG 
GGCTGGAGGC CGCGTAGTGA CAGCCTCATC CTGGACCACC AGTGGGAGCT GGAGAAGCTG 
AGTCTCCTGC AGGAGGTGGA GAAGACCAGG CACTACCTGT TGCTGCGAGA GAAGCTGGAG 
GCGGCCCAGC GGCCCGGCCC CGAGACGCTG TCCCCCGCCG CCAGTGACGG CTCCGAGGCC 
CACGGCTCCT CCAGTGCCTC CTCCCCGCTC ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG 
GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT GTCAAGTGCT TACGGCTCCT CACCCACTCC 
TTCAACAGAG AGTACACCCA CAGCCACGTC TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT 
GAGATGTCGG TCACCCTGCT GCGGGACCCG TCCATGTCCC CGCTGGGGGC CGCCACGCTC 
ACTCCCTCCT CTACCTGCCC CTCCCTGGTC GAGGGCCGGT ATGGGGCCAC CGACCTGAGG 
ACCCCACAGC CCTGCTCCCG GCCCGCCAGC CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT 
GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG 
CTGGTGCCTG ACATCCAGGA AATCCGTGTC AGCCCCATCG TCTCCAAGAA GGGCTACCTG 
CACTTCCTGG AGCCGCACAC GGCCGGCTGG GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC 
TACGCCTACA TGTACAACCA CGACAAGGAC GCCGTGGAGC GCTTCGTGCT CAACCTGTCC 
ACCGCCCAGG TGGAGTACAG CGAGGACCAG CAGGCGATGC TCAAGACCCC CAACACGTTC 
GCGGTGTGCA CGGAGCACCG GGGCATCTTG CTGCAGGCCA GCAGTGACAA GGACATGCAC 
GACTGGCTGT ACGCCTTCAA CCCCCTGCTG GCCGGGACCA TCCGGTCCAA GCTGTCCAGA 
AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318376.1
CDS	323..5689
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462890.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462890.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGAA CCTTTTACCA GTTTGAGGCT 
GCGTGGGACA GCTCCATGCA CAACTCCCTC CTGCTGAACC GGGTCACCCC TTACCGAGAG 
AAGATTTACA TGACCCTCTC TGCTTATATC GAGATGGAGA ACTGCACGCA GCCGGCCGTG 
GTCACCAAAG ACTTCTGCAT GGTCTTCTAC TCGCGCGACG CCAAGCTGCC CGCCTCGCGC 
TCCATCCGCA ACCTGTTCGG CAGTGGGAGC CTGCGGGCCT CAGAGAGCAA CCGCGTGACG 
GGCGTGTACG AGCTGAGTCT GTGCCACGTG GCTGACGCGG GCAGCCCAGG CATGCAGCGG 
CGGCGCCGGC GGGTGCTGGA CACATCCGTG GCCTACGTCC GCGGCGAGGA GAACCTGGCG 
GGCTGGAGGC CGCGTAGTGA CAGCCTCATC CTGGACCACC AGTGGGAGCT GGAGAAGCTG 
AGTCTCCTGC AGGAGGTGGA GAAGACCAGG CACTACCTGT TGCTGCGAGA GAAGCTGGAG 
GCGGCCCAGC GGCCCGGCCC CGAGACGCTG TCCCCCGCCG CCAGTGACGG CTCCGAGGCC 
CACGGCTCCT CCAGTGCCTC CTCCCCGCTC ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG 
GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT GTCAAGTGCT TACGGCTCCT CACCCACTCC 
TTCAACAGAG AGTACACCCA CAGCCACGTC TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT 
GAGATGTCGG TCACCCTGCT GCGGGACCCG TCCATGTCCC CGCTGGGGGC CGCCACGCTC 
ACTCCCTCCT CTACCTGCCC CTCCCTGGTC GAGGGCCGGT ATGGGGCCAC CGACCTGAGG 
ACCCCACAGC CCTGCTCCCG GCCCGCCAGC CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT 
GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG 
CTGGTGCCTG ACATCCAGGA AATCCGTGTC AGCCCCATCG TCTCCAAGAA GGGCTACCTG 
CACTTCCTGG AGCCGCACAC GGCCGGCTGG GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC 
TACGCCTACA TGTACAACCA CGACAAGGAC GCCGTGGAGC GCTTCGTGCT CAACCTGTCC 
ACCGCCCAGG TGGAGTACAG CGAGGACCAG CAGGCGATGC TCAAGACCCC CAACACGTTC 
GCGGTGTGCA CGGAGCACCG GGGCATCTTG CTGCAGGCCA GCAGTGACAA GGACATGCAC 
GACTGGCTGT ACGCCTTCAA CCCCCTGCTG GCCGGGACCA TCCGGTCCAA GCTGTCCAGA 
AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_002686593.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002686639.2 Bos taurus kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

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CloneID	OBa18297
Clone ID Related Accession (Same CDS sequence)	XM_015462890.1 , XM_002686593.5
Accession Version	XM_002686593.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002686593.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGAA CCTTTTACCA GTTTGAGGCT 
GCGTGGGACA GCTCCATGCA CAACTCCCTC CTGCTGAACC GGGTCACCCC TTACCGAGAG 
AAGATTTACA TGACCCTCTC TGCTTATATC GAGATGGAGA ACTGCACGCA GCCGGCCGTG 
GTCACCAAAG ACTTCTGCAT GGTCTTCTAC TCGCGCGACG CCAAGCTGCC CGCCTCGCGC 
TCCATCCGCA ACCTGTTCGG CAGTGGGAGC CTGCGGGCCT CAGAGAGCAA CCGCGTGACG 
GGCGTGTACG AGCTGAGTCT GTGCCACGTG GCTGACGCGG GCAGCCCAGG CATGCAGCGG 
CGGCGCCGGC GGGTGCTGGA CACATCCGTG GCCTACGTCC GCGGCGAGGA GAACCTGGCG 
GGCTGGAGGC CGCGTAGTGA CAGCCTCATC CTGGACCACC AGTGGGAGCT GGAGAAGCTG 
AGTCTCCTGC AGGAGGTGGA GAAGACCAGG CACTACCTGT TGCTGCGAGA GAAGCTGGAG 
GCGGCCCAGC GGCCCGGCCC CGAGACGCTG TCCCCCGCCG CCAGTGACGG CTCCGAGGCC 
CACGGCTCCT CCAGTGCCTC CTCCCCGCTC ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG 
GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT GTCAAGTGCT TACGGCTCCT CACCCACTCC 
TTCAACAGAG AGTACACCCA CAGCCACGTC TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT 
GAGATGTCGG TCACCCTGCT GCGGGACCCG TCCATGTCCC CGCTGGGGGC CGCCACGCTC 
ACTCCCTCCT CTACCTGCCC CTCCCTGGTC GAGGGCCGGT ATGGGGCCAC CGACCTGAGG 
ACCCCACAGC CCTGCTCCCG GCCCGCCAGC CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT 
GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG 
CTGGTGCCTG ACATCCAGGA AATCCGTGTC AGCCCCATCG TCTCCAAGAA GGGCTACCTG 
CACTTCCTGG AGCCGCACAC GGCCGGCTGG GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC 
TACGCCTACA TGTACAACCA CGACAAGGAC GCCGTGGAGC GCTTCGTGCT CAACCTGTCC 
ACCGCCCAGG TGGAGTACAG CGAGGACCAG CAGGCGATGC TCAAGACCCC CAACACGTTC 
GCGGTGTGCA CGGAGCACCG GGGCATCTTG CTGCAGGCCA GCAGTGACAA GGACATGCAC 
GACTGGCTGT ACGCCTTCAA CCCCCTGCTG GCCGGGACCA TCCGGTCCAA GCTGTCCAGA 
AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002686639.2
CDS	323..5689
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRLTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_002686593.5
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002686593.5

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATTGA CCAGCGCCCT GGTGGGCATG AGCCCCTCGT CCTCACTCTC GGCCCTGTCC 
AGCCGTGCCG CCTCCGTGTC CAGCCTGCAC GAGCGCATCT TGTTTGCCCC GGGCAGCGAG 
GAGGCCATCG AAAGGCTGAA GGAGACAGAA AAGATCATCG CTGAGCTCAA CGAGACCTGG 
GAGGAGAAGC TGCGGCGGAC GGAAGCCATC CGGATGGAGA GGGAAGCACT GCTGGCCGAG 
ATGGGCGTGG CCATGCGGGA GGACGGTGGC ACGCTGGGGG TGTTCTCTCC CAAAAAGACG 
CCACATCTGG TCAACCTGAA TGAGGACCCG CTCATGTCCG AGTGCCTGCT CTACTACATC 
AAGGATGGGA TCACCAGGGT GGGCCGGGAG GACGCGGAGA AGCGGCAGGA CATTGTTCTG 
AGCGGGCACT TCATCAAGGA GGAGCACTGT GTCTTCCGGA GCGACTCATG TGGGGGAAGT 
GAAGCTGTGG TGACCCTAGA GCCCTGTGAA GGGGCGGACA CCTATGTCAA TGGCAAGAAA 
GTCACGGAGC CCAGCGTCCT GCGGTCAGGA AACCGCATCA TCATGGGCAA GAGCCACGTG 
TTCCGGTTCA ACCACCCTGA GCAGGCGCGT CAGGAGCGCG AGCGCACGCC CTGCGCAGAG 
ACGCCGGCCG AGCCCGTGGA CTGGGCCTTC GCCCAGCGCG AGCTGCTGGA GAAGCAGGGC 
ATTGACATGA AGCAGGAGAT GGAGCAGAGG CTCCAGGAGC TGGAGGACCA GTACCGCCGG 
GAGCGCGAGG AGGCCACCTA CCTGCTGGAG CAGCAGCGGC TGGACTACGA GAGCAAACTG 
GAGGCCCTGC AGAAGCAGAT GGACTCCAGG TACTATCCCG AGGTGAACGA GGAGGAGGAG 
GAGCCGGAGG ACGAAGTCCA GTGGACCGAG CGAGAGTGCG AGCTGGCACT GTGGGCCTTC 
CGGAAGTGGA AGTGGTACCA GTTCACATCA TTGCGGGACC TGCTGTGGGG CAACGCCATC 
TTCCTCAAGG AGGCCAATGC CATCAGCGTG GAGCTCAAGA AGAAGGTCCA GTTCCAGTTT 
GTCCTCCTCA CGGACACACT CTACTCCCCG CTGCCACCTG ACCTGCTGCC CCCGGAGGCC 
GCCAAAGACC GAGAGACTCG CCCCTTCCCG CGCACCATCG TGGCCGTGGA GGTCCAAGAC 
CAGAAGAACG GGGCCACCCA CTACTGGACG CTGGAGAAAC TCAGGCAGCG GCTAGACCTG 
ATGCGGGAGA TGTATGACCG GGCGGCCGAG GTGCCCTCCA GCATCATCGA GGACTGTGAC 
AACGTGGTGA CCGGAGGAGA CCCCTTCTAC GACCGCTTCC CCTGGTTCCG GCTGGTGGGC 
AGTTCAGTCG TCTCTGGCTG CAACAGCTAT CCTCTTCTCA ACACATGCAT GAGCGAGCGC 
ATGGCTGCTC TCACCCCCTC CCCCACCTTC TCGAGCCCCG ACTCCGACGC CACCGAGCCT 
GCCGAGGAGC AGAGCGTGGG GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG 
GACCTGCAGG ACGACGTCTT TCCGGAGCAC GTGCTGTGCG ACGGCCGGGA CCCGTTTTAC 
GACCGGCCCC CCCTGTTCAG TTTAGTAGGA AGGGCCTTCG TGTACCTCAG CAACCTGCTG 
TACCCCGTGC CGCTGGTGCA CCGCGTGGCC GTCGTCAGCG AGAAGGGCGA GGTGAAGGGC 
TTCCTGCGCG TGGCCGTGCA GGCCATCTCA GCCGACGAGG AGGCCCCTGA CTACGGCTCC 
GGCGTCCGCC AGTCGGGAAC AGCCAAAATC TCCTTTGACG ACCAGCATTT TGAGAAGTTC 
CAGGCCGAGT CCTGCCCCGG GGTGGGCATG TCCCGCTCAG GGACCTCCCA GGAGGAGCTG 
CGGATTGTGG AAGGCCAGGG CCAGGCCGCA GACTCCGGGC CCTCAGCCGA CGAGGTCAAC 
AACAACACCT GTTCAGCGGT GACCCCGGAA GGCCTCCTGG ACAGCCCTGA GAAAGCCGCC 
CTGGATGGGC CCCTGGACGC CGCCCTGGAC CACCTCGGCC TGGGCAGCAC CTTCACCTTC 
CGCGTGACCG TCCTGCAGGC GTCCAGCATA TCCGCTGAAT ATGCCGACAT CTTCTGCCAG 
TTCAACTTCA TCCATCGCCA TGACGAGGCC TTCTCTACGG AGCCCCTGAA GAACACGGGG 
AGGGGACCCC CGCTCGGCTT CTACCACGTC CAAAACATTG CGGTGGAGGT GACCAGGTCC 
TTCATCGAGT ACATCAAGAG CCAGCCCCTC GTGTTCGAGG TCTTCGGGCA CTACCAGCAG 
CACCCGTTCC CGCCCCTCTG CAAGGACGTG CTCAGCCCCC TGAGGCCCTC TCGCCGCCAC 
TTCCCACGGG TCATGCCGCT GTCCAAGCCA GTGCCGGCCA CCAAGCTCAG CACGCTGACG 
CGACCCTGCC CGGGCCCCTG CCACTGCAAG TACGACCTGC TGGTCTACTT CGAGATCTGC 
GAGCTGGAGG CCAACGGCGA CTACATCCCC GCAGTGGTGG ATCACCGCGG CGGGATGCCG 
TGCATGGGGA CCTTCCTGCT CCACCAGGGC ATCCAGAGAC GGATCACCGT GACCCTGCTG 
CACGAGACGG GCAGCCACAT CCGCTGGAAG GAAGTGCGGG AGCTGGTGGT GGGTCGCATC 
CGAAACACCC CGGAAACGGA CGAGTCCCTG ATTGACCCTA ATATCTTGTC GCTCAGCATC 
CTCTCTTCTG GATACATCTG CCCAGCCCAG GATGACCGAA CCTTTTACCA GTTTGAGGCT 
GCGTGGGACA GCTCCATGCA CAACTCCCTC CTGCTGAACC GGGTCACCCC TTACCGAGAG 
AAGATTTACA TGACCCTCTC TGCTTATATC GAGATGGAGA ACTGCACGCA GCCGGCCGTG 
GTCACCAAAG ACTTCTGCAT GGTCTTCTAC TCGCGCGACG CCAAGCTGCC CGCCTCGCGC 
TCCATCCGCA ACCTGTTCGG CAGTGGGAGC CTGCGGGCCT CAGAGAGCAA CCGCGTGACG 
GGCGTGTACG AGCTGAGTCT GTGCCACGTG GCTGACGCGG GCAGCCCAGG CATGCAGCGG 
CGGCGCCGGC GGGTGCTGGA CACATCCGTG GCCTACGTCC GCGGCGAGGA GAACCTGGCG 
GGCTGGAGGC CGCGTAGTGA CAGCCTCATC CTGGACCACC AGTGGGAGCT GGAGAAGCTG 
AGTCTCCTGC AGGAGGTGGA GAAGACCAGG CACTACCTGT TGCTGCGAGA GAAGCTGGAG 
GCGGCCCAGC GGCCCGGCCC CGAGACGCTG TCCCCCGCCG CCAGTGACGG CTCCGAGGCC 
CACGGCTCCT CCAGTGCCTC CTCCCCGCTC ACGGCTGAGG CCCGGCCAGC ATCCCTGGAG 
GCGCCCAGCG AGCGGCAGCG GGAGCTGGCT GTCAAGTGCT TACGGCTCCT CACCCACTCC 
TTCAACAGAG AGTACACCCA CAGCCACGTC TGCGTCAGCG CCAGCGAGAG CAAGCTCTCT 
GAGATGTCGG TCACCCTGCT GCGGGACCCG TCCATGTCCC CGCTGGGGGC CGCCACGCTC 
ACTCCCTCCT CTACCTGCCC CTCCCTGGTC GAGGGCCGGT ATGGGGCCAC CGACCTGAGG 
ACCCCACAGC CCTGCTCCCG GCCCGCCAGC CCGGAGCCTG AGCCCCTGCC CGAGGTAGAT 
GCCAAGAAGC TCCCCTCCCC TGCCCGGGCA GCGGAAGCCG ACAAGGAGCC CCAGCGCCTG 
CTGGTGCCTG ACATCCAGGA AATCCGTGTC AGCCCCATCG TCTCCAAGAA GGGCTACCTG 
CACTTCCTGG AGCCGCACAC GGCCGGCTGG GCCAAGCGCT TCGTGGTGGT GCGGCGGCCC 
TACGCCTACA TGTACAACCA CGACAAGGAC GCCGTGGAGC GCTTCGTGCT CAACCTGTCC 
ACCGCCCAGG TGGAGTACAG CGAGGACCAG CAGGCGATGC TCAAGACCCC CAACACGTTC 
GCGGTGTGCA CGGAGCACCG GGGCATCTTG CTGCAGGCCA GCAGTGACAA GGACATGCAC 
GACTGGCTGT ACGCCTTCAA CCCCCTGCTG GCCGGGACCA TCCGGTCCAA GCTGTCCAGA 
AGGAGGTCTG CCCAGATGCG GGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462883.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318369.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

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CloneID	OBa51455
Clone ID Related Accession (Same CDS sequence)	XM_015462883.1 , XM_010803923.2
Accession Version	XM_015462883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGGCAG 
TTTCTTGATT CGGACATGCC TAGCGTTTCG TTTGGAAGTG ACACTAGAAC CTTTTACCAG 
TTTGAGGCTG CGTGGGACAG CTCCATGCAC AACTCCCTCC TGCTGAACCG GGTCACCCCT 
TACCGAGAGA AGATTTACAT GACCCTCTCT GCTTATATCG AGATGGAGAA CTGCACGCAG 
CCGGCCGTGG TCACCAAAGA CTTCTGCATG GTCTTCTACT CGCGCGACGC CAAGCTGCCC 
GCCTCGCGCT CCATCCGCAA CCTGTTCGGC AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC 
CGCGTGACGG GCGTGTACGA GCTGAGTCTG TGCCACGTGG CTGACGCGGG CAGCCCAGGC 
ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC ACATCCGTGG CCTACGTCCG CGGCGAGGAG 
AACCTGGCGG GCTGGAGGCC GCGTAGTGAC AGCCTCATCC TGGACCACCA GTGGGAGCTG 
GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG AAGACCAGGC ACTACCTGTT GCTGCGAGAG 
AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC GAGACGCTGT CCCCCGCCGC CAGTGACGGC 
TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA 
TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC 
ACCCACTCCT TCAACAGAGA GTACACCCAC AGCCACGTCT GCGTCAGCGC CAGCGAGAGC 
AAGCTCTCTG AGATGTCGGT CACCCTGCTG CGGGACCCGT CCATGTCCCC GCTGGGGGCC 
GCCACGCTCA CTCCCTCCTC TACCTGCCCC TCCCTGGTCG AGGGCCGGTA TGGGGCCACC 
GACCTGAGGA CCCCACAGCC CTGCTCCCGG CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC 
GAGGTAGATG CCAAGAAGCT CCCCTCCCCT GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC 
CAGCGCCTGC TGGTGCCTGA CATCCAGGAA ATCCGTGTCA GCCCCATCGT CTCCAAGAAG 
GGCTACCTGC ACTTCCTGGA GCCGCACACG GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG 
CGGCGGCCCT ACGCCTACAT GTACAACCAC GACAAGGACG CCGTGGAGCG CTTCGTGCTC 
AACCTGTCCA CCGCCCAGGT GGAGTACAGC GAGGACCAGC AGGCGATGCT CAAGACCCCC 
AACACGTTCG CGGTGTGCAC GGAGCACCGG GGCATCTTGC TGCAGGCCAG CAGTGACAAG 
GACATGCACG ACTGGCTGTA CGCCTTCAAC CCCCTGCTGG CCGGGACCAT CCGGTCCAAG 
CTGTCCAGAA GGAGGTCTGC CCAGATGCGG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318369.1
CDS	323..5818
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462883.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462883.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGGCAG 
TTTCTTGATT CGGACATGCC TAGCGTTTCG TTTGGAAGTG ACACTAGAAC CTTTTACCAG 
TTTGAGGCTG CGTGGGACAG CTCCATGCAC AACTCCCTCC TGCTGAACCG GGTCACCCCT 
TACCGAGAGA AGATTTACAT GACCCTCTCT GCTTATATCG AGATGGAGAA CTGCACGCAG 
CCGGCCGTGG TCACCAAAGA CTTCTGCATG GTCTTCTACT CGCGCGACGC CAAGCTGCCC 
GCCTCGCGCT CCATCCGCAA CCTGTTCGGC AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC 
CGCGTGACGG GCGTGTACGA GCTGAGTCTG TGCCACGTGG CTGACGCGGG CAGCCCAGGC 
ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC ACATCCGTGG CCTACGTCCG CGGCGAGGAG 
AACCTGGCGG GCTGGAGGCC GCGTAGTGAC AGCCTCATCC TGGACCACCA GTGGGAGCTG 
GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG AAGACCAGGC ACTACCTGTT GCTGCGAGAG 
AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC GAGACGCTGT CCCCCGCCGC CAGTGACGGC 
TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA 
TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC 
ACCCACTCCT TCAACAGAGA GTACACCCAC AGCCACGTCT GCGTCAGCGC CAGCGAGAGC 
AAGCTCTCTG AGATGTCGGT CACCCTGCTG CGGGACCCGT CCATGTCCCC GCTGGGGGCC 
GCCACGCTCA CTCCCTCCTC TACCTGCCCC TCCCTGGTCG AGGGCCGGTA TGGGGCCACC 
GACCTGAGGA CCCCACAGCC CTGCTCCCGG CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC 
GAGGTAGATG CCAAGAAGCT CCCCTCCCCT GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC 
CAGCGCCTGC TGGTGCCTGA CATCCAGGAA ATCCGTGTCA GCCCCATCGT CTCCAAGAAG 
GGCTACCTGC ACTTCCTGGA GCCGCACACG GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG 
CGGCGGCCCT ACGCCTACAT GTACAACCAC GACAAGGACG CCGTGGAGCG CTTCGTGCTC 
AACCTGTCCA CCGCCCAGGT GGAGTACAGC GAGGACCAGC AGGCGATGCT CAAGACCCCC 
AACACGTTCG CGGTGTGCAC GGAGCACCGG GGCATCTTGC TGCAGGCCAG CAGTGACAAG 
GACATGCACG ACTGGCTGTA CGCCTTCAAC CCCCTGCTGG CCGGGACCAT CCGGTCCAAG 
CTGTCCAGAA GGAGGTCTGC CCAGATGCGG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803923.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802225.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

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CloneID	OBa51455
Clone ID Related Accession (Same CDS sequence)	XM_015462883.1 , XM_010803923.2
Accession Version	XM_010803923.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGGCAG 
TTTCTTGATT CGGACATGCC TAGCGTTTCG TTTGGAAGTG ACACTAGAAC CTTTTACCAG 
TTTGAGGCTG CGTGGGACAG CTCCATGCAC AACTCCCTCC TGCTGAACCG GGTCACCCCT 
TACCGAGAGA AGATTTACAT GACCCTCTCT GCTTATATCG AGATGGAGAA CTGCACGCAG 
CCGGCCGTGG TCACCAAAGA CTTCTGCATG GTCTTCTACT CGCGCGACGC CAAGCTGCCC 
GCCTCGCGCT CCATCCGCAA CCTGTTCGGC AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC 
CGCGTGACGG GCGTGTACGA GCTGAGTCTG TGCCACGTGG CTGACGCGGG CAGCCCAGGC 
ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC ACATCCGTGG CCTACGTCCG CGGCGAGGAG 
AACCTGGCGG GCTGGAGGCC GCGTAGTGAC AGCCTCATCC TGGACCACCA GTGGGAGCTG 
GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG AAGACCAGGC ACTACCTGTT GCTGCGAGAG 
AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC GAGACGCTGT CCCCCGCCGC CAGTGACGGC 
TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA 
TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC 
ACCCACTCCT TCAACAGAGA GTACACCCAC AGCCACGTCT GCGTCAGCGC CAGCGAGAGC 
AAGCTCTCTG AGATGTCGGT CACCCTGCTG CGGGACCCGT CCATGTCCCC GCTGGGGGCC 
GCCACGCTCA CTCCCTCCTC TACCTGCCCC TCCCTGGTCG AGGGCCGGTA TGGGGCCACC 
GACCTGAGGA CCCCACAGCC CTGCTCCCGG CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC 
GAGGTAGATG CCAAGAAGCT CCCCTCCCCT GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC 
CAGCGCCTGC TGGTGCCTGA CATCCAGGAA ATCCGTGTCA GCCCCATCGT CTCCAAGAAG 
GGCTACCTGC ACTTCCTGGA GCCGCACACG GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG 
CGGCGGCCCT ACGCCTACAT GTACAACCAC GACAAGGACG CCGTGGAGCG CTTCGTGCTC 
AACCTGTCCA CCGCCCAGGT GGAGTACAGC GAGGACCAGC AGGCGATGCT CAAGACCCCC 
AACACGTTCG CGGTGTGCAC GGAGCACCGG GGCATCTTGC TGCAGGCCAG CAGTGACAAG 
GACATGCACG ACTGGCTGTA CGCCTTCAAC CCCCTGCTGG CCGGGACCAT CCGGTCCAAG 
CTGTCCAGAA GGAGGTCTGC CCAGATGCGG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802225.1
CDS	323..5818
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803923.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803923.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGGCAG 
TTTCTTGATT CGGACATGCC TAGCGTTTCG TTTGGAAGTG ACACTAGAAC CTTTTACCAG 
TTTGAGGCTG CGTGGGACAG CTCCATGCAC AACTCCCTCC TGCTGAACCG GGTCACCCCT 
TACCGAGAGA AGATTTACAT GACCCTCTCT GCTTATATCG AGATGGAGAA CTGCACGCAG 
CCGGCCGTGG TCACCAAAGA CTTCTGCATG GTCTTCTACT CGCGCGACGC CAAGCTGCCC 
GCCTCGCGCT CCATCCGCAA CCTGTTCGGC AGTGGGAGCC TGCGGGCCTC AGAGAGCAAC 
CGCGTGACGG GCGTGTACGA GCTGAGTCTG TGCCACGTGG CTGACGCGGG CAGCCCAGGC 
ATGCAGCGGC GGCGCCGGCG GGTGCTGGAC ACATCCGTGG CCTACGTCCG CGGCGAGGAG 
AACCTGGCGG GCTGGAGGCC GCGTAGTGAC AGCCTCATCC TGGACCACCA GTGGGAGCTG 
GAGAAGCTGA GTCTCCTGCA GGAGGTGGAG AAGACCAGGC ACTACCTGTT GCTGCGAGAG 
AAGCTGGAGG CGGCCCAGCG GCCCGGCCCC GAGACGCTGT CCCCCGCCGC CAGTGACGGC 
TCCGAGGCCC ACGGCTCCTC CAGTGCCTCC TCCCCGCTCA CGGCTGAGGC CCGGCCAGCA 
TCCCTGGAGG CGCCCAGCGA GCGGCAGCGG GAGCTGGCTG TCAAGTGCTT ACGGCTCCTC 
ACCCACTCCT TCAACAGAGA GTACACCCAC AGCCACGTCT GCGTCAGCGC CAGCGAGAGC 
AAGCTCTCTG AGATGTCGGT CACCCTGCTG CGGGACCCGT CCATGTCCCC GCTGGGGGCC 
GCCACGCTCA CTCCCTCCTC TACCTGCCCC TCCCTGGTCG AGGGCCGGTA TGGGGCCACC 
GACCTGAGGA CCCCACAGCC CTGCTCCCGG CCCGCCAGCC CGGAGCCTGA GCCCCTGCCC 
GAGGTAGATG CCAAGAAGCT CCCCTCCCCT GCCCGGGCAG CGGAAGCCGA CAAGGAGCCC 
CAGCGCCTGC TGGTGCCTGA CATCCAGGAA ATCCGTGTCA GCCCCATCGT CTCCAAGAAG 
GGCTACCTGC ACTTCCTGGA GCCGCACACG GCCGGCTGGG CCAAGCGCTT CGTGGTGGTG 
CGGCGGCCCT ACGCCTACAT GTACAACCAC GACAAGGACG CCGTGGAGCG CTTCGTGCTC 
AACCTGTCCA CCGCCCAGGT GGAGTACAGC GAGGACCAGC AGGCGATGCT CAAGACCCCC 
AACACGTTCG CGGTGTGCAC GGAGCACCGG GGCATCTTGC TGCAGGCCAG CAGTGACAAG 
GACATGCACG ACTGGCTGTA CGCCTTCAAC CCCCTGCTGG CCGGGACCAT CCGGTCCAAG 
CTGTCCAGAA GGAGGTCTGC CCAGATGCGG GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462884.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318370.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

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CloneID	OBa51456
Clone ID Related Accession (Same CDS sequence)	XM_015462884.1 , XM_010803924.2
Accession Version	XM_015462884.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGGCAG 
TTTCTTGATT CGGACATGCC TAGAACCTTT TACCAGTTTG AGGCTGCGTG GGACAGCTCC 
ATGCACAACT CCCTCCTGCT GAACCGGGTC ACCCCTTACC GAGAGAAGAT TTACATGACC 
CTCTCTGCTT ATATCGAGAT GGAGAACTGC ACGCAGCCGG CCGTGGTCAC CAAAGACTTC 
TGCATGGTCT TCTACTCGCG CGACGCCAAG CTGCCCGCCT CGCGCTCCAT CCGCAACCTG 
TTCGGCAGTG GGAGCCTGCG GGCCTCAGAG AGCAACCGCG TGACGGGCGT GTACGAGCTG 
AGTCTGTGCC ACGTGGCTGA CGCGGGCAGC CCAGGCATGC AGCGGCGGCG CCGGCGGGTG 
CTGGACACAT CCGTGGCCTA CGTCCGCGGC GAGGAGAACC TGGCGGGCTG GAGGCCGCGT 
AGTGACAGCC TCATCCTGGA CCACCAGTGG GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG 
GTGGAGAAGA CCAGGCACTA CCTGTTGCTG CGAGAGAAGC TGGAGGCGGC CCAGCGGCCC 
GGCCCCGAGA CGCTGTCCCC CGCCGCCAGT GACGGCTCCG AGGCCCACGG CTCCTCCAGT 
GCCTCCTCCC CGCTCACGGC TGAGGCCCGG CCAGCATCCC TGGAGGCGCC CAGCGAGCGG 
CAGCGGGAGC TGGCTGTCAA GTGCTTACGG CTCCTCACCC ACTCCTTCAA CAGAGAGTAC 
ACCCACAGCC ACGTCTGCGT CAGCGCCAGC GAGAGCAAGC TCTCTGAGAT GTCGGTCACC 
CTGCTGCGGG ACCCGTCCAT GTCCCCGCTG GGGGCCGCCA CGCTCACTCC CTCCTCTACC 
TGCCCCTCCC TGGTCGAGGG CCGGTATGGG GCCACCGACC TGAGGACCCC ACAGCCCTGC 
TCCCGGCCCG CCAGCCCGGA GCCTGAGCCC CTGCCCGAGG TAGATGCCAA GAAGCTCCCC 
TCCCCTGCCC GGGCAGCGGA AGCCGACAAG GAGCCCCAGC GCCTGCTGGT GCCTGACATC 
CAGGAAATCC GTGTCAGCCC CATCGTCTCC AAGAAGGGCT ACCTGCACTT CCTGGAGCCG 
CACACGGCCG GCTGGGCCAA GCGCTTCGTG GTGGTGCGGC GGCCCTACGC CTACATGTAC 
AACCACGACA AGGACGCCGT GGAGCGCTTC GTGCTCAACC TGTCCACCGC CCAGGTGGAG 
TACAGCGAGG ACCAGCAGGC GATGCTCAAG ACCCCCAACA CGTTCGCGGT GTGCACGGAG 
CACCGGGGCA TCTTGCTGCA GGCCAGCAGT GACAAGGACA TGCACGACTG GCTGTACGCC 
TTCAACCCCC TGCTGGCCGG GACCATCCGG TCCAAGCTGT CCAGAAGGAG GTCTGCCCAG 
ATGCGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318370.1
CDS	323..5794
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462884.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462884.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGGCAG 
TTTCTTGATT CGGACATGCC TAGAACCTTT TACCAGTTTG AGGCTGCGTG GGACAGCTCC 
ATGCACAACT CCCTCCTGCT GAACCGGGTC ACCCCTTACC GAGAGAAGAT TTACATGACC 
CTCTCTGCTT ATATCGAGAT GGAGAACTGC ACGCAGCCGG CCGTGGTCAC CAAAGACTTC 
TGCATGGTCT TCTACTCGCG CGACGCCAAG CTGCCCGCCT CGCGCTCCAT CCGCAACCTG 
TTCGGCAGTG GGAGCCTGCG GGCCTCAGAG AGCAACCGCG TGACGGGCGT GTACGAGCTG 
AGTCTGTGCC ACGTGGCTGA CGCGGGCAGC CCAGGCATGC AGCGGCGGCG CCGGCGGGTG 
CTGGACACAT CCGTGGCCTA CGTCCGCGGC GAGGAGAACC TGGCGGGCTG GAGGCCGCGT 
AGTGACAGCC TCATCCTGGA CCACCAGTGG GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG 
GTGGAGAAGA CCAGGCACTA CCTGTTGCTG CGAGAGAAGC TGGAGGCGGC CCAGCGGCCC 
GGCCCCGAGA CGCTGTCCCC CGCCGCCAGT GACGGCTCCG AGGCCCACGG CTCCTCCAGT 
GCCTCCTCCC CGCTCACGGC TGAGGCCCGG CCAGCATCCC TGGAGGCGCC CAGCGAGCGG 
CAGCGGGAGC TGGCTGTCAA GTGCTTACGG CTCCTCACCC ACTCCTTCAA CAGAGAGTAC 
ACCCACAGCC ACGTCTGCGT CAGCGCCAGC GAGAGCAAGC TCTCTGAGAT GTCGGTCACC 
CTGCTGCGGG ACCCGTCCAT GTCCCCGCTG GGGGCCGCCA CGCTCACTCC CTCCTCTACC 
TGCCCCTCCC TGGTCGAGGG CCGGTATGGG GCCACCGACC TGAGGACCCC ACAGCCCTGC 
TCCCGGCCCG CCAGCCCGGA GCCTGAGCCC CTGCCCGAGG TAGATGCCAA GAAGCTCCCC 
TCCCCTGCCC GGGCAGCGGA AGCCGACAAG GAGCCCCAGC GCCTGCTGGT GCCTGACATC 
CAGGAAATCC GTGTCAGCCC CATCGTCTCC AAGAAGGGCT ACCTGCACTT CCTGGAGCCG 
CACACGGCCG GCTGGGCCAA GCGCTTCGTG GTGGTGCGGC GGCCCTACGC CTACATGTAC 
AACCACGACA AGGACGCCGT GGAGCGCTTC GTGCTCAACC TGTCCACCGC CCAGGTGGAG 
TACAGCGAGG ACCAGCAGGC GATGCTCAAG ACCCCCAACA CGTTCGCGGT GTGCACGGAG 
CACCGGGGCA TCTTGCTGCA GGCCAGCAGT GACAAGGACA TGCACGACTG GCTGTACGCC 
TTCAACCCCC TGCTGGCCGG GACCATCCGG TCCAAGCTGT CCAGAAGGAG GTCTGCCCAG 
ATGCGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803924.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802226.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

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CloneID	OBa51456
Clone ID Related Accession (Same CDS sequence)	XM_015462884.1 , XM_010803924.2
Accession Version	XM_010803924.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803924.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGGCAG 
TTTCTTGATT CGGACATGCC TAGAACCTTT TACCAGTTTG AGGCTGCGTG GGACAGCTCC 
ATGCACAACT CCCTCCTGCT GAACCGGGTC ACCCCTTACC GAGAGAAGAT TTACATGACC 
CTCTCTGCTT ATATCGAGAT GGAGAACTGC ACGCAGCCGG CCGTGGTCAC CAAAGACTTC 
TGCATGGTCT TCTACTCGCG CGACGCCAAG CTGCCCGCCT CGCGCTCCAT CCGCAACCTG 
TTCGGCAGTG GGAGCCTGCG GGCCTCAGAG AGCAACCGCG TGACGGGCGT GTACGAGCTG 
AGTCTGTGCC ACGTGGCTGA CGCGGGCAGC CCAGGCATGC AGCGGCGGCG CCGGCGGGTG 
CTGGACACAT CCGTGGCCTA CGTCCGCGGC GAGGAGAACC TGGCGGGCTG GAGGCCGCGT 
AGTGACAGCC TCATCCTGGA CCACCAGTGG GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG 
GTGGAGAAGA CCAGGCACTA CCTGTTGCTG CGAGAGAAGC TGGAGGCGGC CCAGCGGCCC 
GGCCCCGAGA CGCTGTCCCC CGCCGCCAGT GACGGCTCCG AGGCCCACGG CTCCTCCAGT 
GCCTCCTCCC CGCTCACGGC TGAGGCCCGG CCAGCATCCC TGGAGGCGCC CAGCGAGCGG 
CAGCGGGAGC TGGCTGTCAA GTGCTTACGG CTCCTCACCC ACTCCTTCAA CAGAGAGTAC 
ACCCACAGCC ACGTCTGCGT CAGCGCCAGC GAGAGCAAGC TCTCTGAGAT GTCGGTCACC 
CTGCTGCGGG ACCCGTCCAT GTCCCCGCTG GGGGCCGCCA CGCTCACTCC CTCCTCTACC 
TGCCCCTCCC TGGTCGAGGG CCGGTATGGG GCCACCGACC TGAGGACCCC ACAGCCCTGC 
TCCCGGCCCG CCAGCCCGGA GCCTGAGCCC CTGCCCGAGG TAGATGCCAA GAAGCTCCCC 
TCCCCTGCCC GGGCAGCGGA AGCCGACAAG GAGCCCCAGC GCCTGCTGGT GCCTGACATC 
CAGGAAATCC GTGTCAGCCC CATCGTCTCC AAGAAGGGCT ACCTGCACTT CCTGGAGCCG 
CACACGGCCG GCTGGGCCAA GCGCTTCGTG GTGGTGCGGC GGCCCTACGC CTACATGTAC 
AACCACGACA AGGACGCCGT GGAGCGCTTC GTGCTCAACC TGTCCACCGC CCAGGTGGAG 
TACAGCGAGG ACCAGCAGGC GATGCTCAAG ACCCCCAACA CGTTCGCGGT GTGCACGGAG 
CACCGGGGCA TCTTGCTGCA GGCCAGCAGT GACAAGGACA TGCACGACTG GCTGTACGCC 
TTCAACCCCC TGCTGGCCGG GACCATCCGG TCCAAGCTGT CCAGAAGGAG GTCTGCCCAG 
ATGCGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802226.1
CDS	323..5794
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803924.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803924.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGGCAG 
TTTCTTGATT CGGACATGCC TAGAACCTTT TACCAGTTTG AGGCTGCGTG GGACAGCTCC 
ATGCACAACT CCCTCCTGCT GAACCGGGTC ACCCCTTACC GAGAGAAGAT TTACATGACC 
CTCTCTGCTT ATATCGAGAT GGAGAACTGC ACGCAGCCGG CCGTGGTCAC CAAAGACTTC 
TGCATGGTCT TCTACTCGCG CGACGCCAAG CTGCCCGCCT CGCGCTCCAT CCGCAACCTG 
TTCGGCAGTG GGAGCCTGCG GGCCTCAGAG AGCAACCGCG TGACGGGCGT GTACGAGCTG 
AGTCTGTGCC ACGTGGCTGA CGCGGGCAGC CCAGGCATGC AGCGGCGGCG CCGGCGGGTG 
CTGGACACAT CCGTGGCCTA CGTCCGCGGC GAGGAGAACC TGGCGGGCTG GAGGCCGCGT 
AGTGACAGCC TCATCCTGGA CCACCAGTGG GAGCTGGAGA AGCTGAGTCT CCTGCAGGAG 
GTGGAGAAGA CCAGGCACTA CCTGTTGCTG CGAGAGAAGC TGGAGGCGGC CCAGCGGCCC 
GGCCCCGAGA CGCTGTCCCC CGCCGCCAGT GACGGCTCCG AGGCCCACGG CTCCTCCAGT 
GCCTCCTCCC CGCTCACGGC TGAGGCCCGG CCAGCATCCC TGGAGGCGCC CAGCGAGCGG 
CAGCGGGAGC TGGCTGTCAA GTGCTTACGG CTCCTCACCC ACTCCTTCAA CAGAGAGTAC 
ACCCACAGCC ACGTCTGCGT CAGCGCCAGC GAGAGCAAGC TCTCTGAGAT GTCGGTCACC 
CTGCTGCGGG ACCCGTCCAT GTCCCCGCTG GGGGCCGCCA CGCTCACTCC CTCCTCTACC 
TGCCCCTCCC TGGTCGAGGG CCGGTATGGG GCCACCGACC TGAGGACCCC ACAGCCCTGC 
TCCCGGCCCG CCAGCCCGGA GCCTGAGCCC CTGCCCGAGG TAGATGCCAA GAAGCTCCCC 
TCCCCTGCCC GGGCAGCGGA AGCCGACAAG GAGCCCCAGC GCCTGCTGGT GCCTGACATC 
CAGGAAATCC GTGTCAGCCC CATCGTCTCC AAGAAGGGCT ACCTGCACTT CCTGGAGCCG 
CACACGGCCG GCTGGGCCAA GCGCTTCGTG GTGGTGCGGC GGCCCTACGC CTACATGTAC 
AACCACGACA AGGACGCCGT GGAGCGCTTC GTGCTCAACC TGTCCACCGC CCAGGTGGAG 
TACAGCGAGG ACCAGCAGGC GATGCTCAAG ACCCCCAACA CGTTCGCGGT GTGCACGGAG 
CACCGGGGCA TCTTGCTGCA GGCCAGCAGT GACAAGGACA TGCACGACTG GCTGTACGCC 
TTCAACCCCC TGCTGGCCGG GACCATCCGG TCCAAGCTGT CCAGAAGGAG GTCTGCCCAG 
ATGCGGGTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803925.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802227.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

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CloneID	OBa51457
Clone ID Related Accession (Same CDS sequence)	XM_010803925.2 , XM_015462885.1
Accession Version	XM_010803925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGCGTT 
TCGTTTGGAA GTGACACTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802227.1
CDS	323..5791
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803925.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803925.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGCGTT 
TCGTTTGGAA GTGACACTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462885.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318371.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

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CloneID	OBa51457
Clone ID Related Accession (Same CDS sequence)	XM_010803925.2 , XM_015462885.1
Accession Version	XM_015462885.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGCGTT 
TCGTTTGGAA GTGACACTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318371.1
CDS	323..5791
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462885.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462885.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGCGTT 
TCGTTTGGAA GTGACACTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462886.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318372.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

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CloneID	OBa51458
Clone ID Related Accession (Same CDS sequence)	XM_015462886.1 , XM_010803926.2
Accession Version	XM_015462886.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA ACGTGTCCGA CCTTGAGAAC 
AATAACCGTA GCCGTGGCGG GGTGGAGCTG AACCCAGCCC CTGACAATCT CTCCACAGTG 
ACCAGCGCCC TGGTGGGCAT GAGCCCCTCG TCCTCACTCT CGGCCCTGTC CAGCCGTGCC 
GCCTCCGTGT CCAGCCTGCA CGAGCGCATC TTGTTTGCCC CGGGCAGCGA GGAGGCCATC 
GAAAGGCTGA AGGAGACAGA AAAGATCATC GCTGAGCTCA ACGAGACCTG GGAGGAGAAG 
CTGCGGCGGA CGGAAGCCAT CCGGATGGAG AGGGAAGCAC TGCTGGCCGA GATGGGCGTG 
GCCATGCGGG AGGACGGTGG CACGCTGGGG GTGTTCTCTC CCAAAAAGAC GCCACATCTG 
GTCAACCTGA ATGAGGACCC GCTCATGTCC GAGTGCCTGC TCTACTACAT CAAGGATGGG 
ATCACCAGGG TGGGCCGGGA GGACGCGGAG AAGCGGCAGG ACATTGTTCT GAGCGGGCAC 
TTCATCAAGG AGGAGCACTG TGTCTTCCGG AGCGACTCAT GTGGGGGAAG TGAAGCTGTG 
GTGACCCTAG AGCCCTGTGA AGGGGCGGAC ACCTATGTCA ATGGCAAGAA AGTCACGGAG 
CCCAGCGTCC TGCGGTCAGG AAACCGCATC ATCATGGGCA AGAGCCACGT GTTCCGGTTC 
AACCACCCTG AGCAGGCGCG TCAGGAGCGC GAGCGCACGC CCTGCGCAGA GACGCCGGCC 
GAGCCCGTGG ACTGGGCCTT CGCCCAGCGC GAGCTGCTGG AGAAGCAGGG CATTGACATG 
AAGCAGGAGA TGGAGCAGAG GCTCCAGGAG CTGGAGGACC AGTACCGCCG GGAGCGCGAG 
GAGGCCACCT ACCTGCTGGA GCAGCAGCGG CTGGACTACG AGAGCAAACT GGAGGCCCTG 
CAGAAGCAGA TGGACTCCAG GTACTATCCC GAGGTGAACG AGGAGGAGGA GGAGCCGGAG 
GACGAAGTCC AGTGGACCGA GCGAGAGTGC GAGCTGGCAC TGTGGGCCTT CCGGAAGTGG 
AAGTGGTACC AGTTCACATC ATTGCGGGAC CTGCTGTGGG GCAACGCCAT CTTCCTCAAG 
GAGGCCAATG CCATCAGCGT GGAGCTCAAG AAGAAGGTCC AGTTCCAGTT TGTCCTCCTC 
ACGGACACAC TCTACTCCCC GCTGCCACCT GACCTGCTGC CCCCGGAGGC CGCCAAAGAC 
CGAGAGACTC GCCCCTTCCC GCGCACCATC GTGGCCGTGG AGGTCCAAGA CCAGAAGAAC 
GGGGCCACCC ACTACTGGAC GCTGGAGAAA CTCAGGCAGC GGCTAGACCT GATGCGGGAG 
ATGTATGACC GGGCGGCCGA GGTGCCCTCC AGCATCATCG AGGACTGTGA CAACGTGGTG 
ACCGGAGGAG ACCCCTTCTA CGACCGCTTC CCCTGGTTCC GGCTGGTGGG CAGTTCAGTC 
GTCTCTGGCT GCAACAGCTA TCCTCTTCTC AACACATGCA TGAGCGAGCG CATGGCTGCT 
CTCACCCCCT CCCCCACCTT CTCGAGCCCC GACTCCGACG CCACCGAGCC TGCCGAGGAG 
CAGAGCGTGG GGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGACCTGCAG 
GACGACGTCT TTCCGGAGCA CGTGCTGTGC GACGGCCGGG ACCCGTTTTA CGACCGGCCC 
CCCCTGTTCA GTTTAGTAGG AAGGGCCTTC GTGTACCTCA GCAACCTGCT GTACCCCGTG 
CCGCTGGTGC ACCGCGTGGC CGTCGTCAGC GAGAAGGGCG AGGTGAAGGG CTTCCTGCGC 
GTGGCCGTGC AGGCCATCTC AGCCGACGAG GAGGCCCCTG ACTACGGCTC CGGCGTCCGC 
CAGTCGGGAA CAGCCAAAAT CTCCTTTGAC GACCAGCATT TTGAGAAGTT CCAGGCCGAG 
TCCTGCCCCG GGGTGGGCAT GTCCCGCTCA GGGACCTCCC AGGAGGAGCT GCGGATTGTG 
GAAGGCCAGG GCCAGGCCGC AGACTCCGGG CCCTCAGCCG ACGAGGTCAA CAACAACACC 
TGTTCAGCGG TGACCCCGGA AGGCCTCCTG GACAGCCCTG AGAAAGCCGC CCTGGATGGG 
CCCCTGGACG CCGCCCTGGA CCACCTCGGC CTGGGCAGCA CCTTCACCTT CCGCGTGACC 
GTCCTGCAGG CGTCCAGCAT ATCCGCTGAA TATGCCGACA TCTTCTGCCA GTTCAACTTC 
ATCCATCGCC ATGACGAGGC CTTCTCTACG GAGCCCCTGA AGAACACGGG GAGGGGACCC 
CCGCTCGGCT TCTACCACGT CCAAAACATT GCGGTGGAGG TGACCAGGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCCT CGTGTTCGAG GTCTTCGGGC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGACGT GCTCAGCCCC CTGAGGCCCT CTCGCCGCCA CTTCCCACGG 
GTCATGCCGC TGTCCAAGCC AGTGCCGGCC ACCAAGCTCA GCACGCTGAC GCGACCCTGC 
CCGGGCCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TCGAGATCTG CGAGCTGGAG 
GCCAACGGCG ACTACATCCC CGCAGTGGTG GATCACCGCG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TCCACCAGGG CATCCAGAGA CGGATCACCG TGACCCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTGCGG GAGCTGGTGG TGGGTCGCAT CCGAAACACC 
CCGGAAACGG ACGAGTCCCT GATTGACCCT AATATCTTGT CGCTCAGCAT CCTCTCTTCT 
GGATACATCT GCCCAGCCCA GGATGACCGG CAGTTTCTTG ATTCGGACAT GCCTAGCGTT 
TCGTTTGGAA GTGACACTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318372.1
CDS	323..5791
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDNVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462886.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462886.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA ACGTGTCCGA CCTTGAGAAC 
AATAACCGTA GCCGTGGCGG GGTGGAGCTG AACCCAGCCC CTGACAATCT CTCCACAGTG 
ACCAGCGCCC TGGTGGGCAT GAGCCCCTCG TCCTCACTCT CGGCCCTGTC CAGCCGTGCC 
GCCTCCGTGT CCAGCCTGCA CGAGCGCATC TTGTTTGCCC CGGGCAGCGA GGAGGCCATC 
GAAAGGCTGA AGGAGACAGA AAAGATCATC GCTGAGCTCA ACGAGACCTG GGAGGAGAAG 
CTGCGGCGGA CGGAAGCCAT CCGGATGGAG AGGGAAGCAC TGCTGGCCGA GATGGGCGTG 
GCCATGCGGG AGGACGGTGG CACGCTGGGG GTGTTCTCTC CCAAAAAGAC GCCACATCTG 
GTCAACCTGA ATGAGGACCC GCTCATGTCC GAGTGCCTGC TCTACTACAT CAAGGATGGG 
ATCACCAGGG TGGGCCGGGA GGACGCGGAG AAGCGGCAGG ACATTGTTCT GAGCGGGCAC 
TTCATCAAGG AGGAGCACTG TGTCTTCCGG AGCGACTCAT GTGGGGGAAG TGAAGCTGTG 
GTGACCCTAG AGCCCTGTGA AGGGGCGGAC ACCTATGTCA ATGGCAAGAA AGTCACGGAG 
CCCAGCGTCC TGCGGTCAGG AAACCGCATC ATCATGGGCA AGAGCCACGT GTTCCGGTTC 
AACCACCCTG AGCAGGCGCG TCAGGAGCGC GAGCGCACGC CCTGCGCAGA GACGCCGGCC 
GAGCCCGTGG ACTGGGCCTT CGCCCAGCGC GAGCTGCTGG AGAAGCAGGG CATTGACATG 
AAGCAGGAGA TGGAGCAGAG GCTCCAGGAG CTGGAGGACC AGTACCGCCG GGAGCGCGAG 
GAGGCCACCT ACCTGCTGGA GCAGCAGCGG CTGGACTACG AGAGCAAACT GGAGGCCCTG 
CAGAAGCAGA TGGACTCCAG GTACTATCCC GAGGTGAACG AGGAGGAGGA GGAGCCGGAG 
GACGAAGTCC AGTGGACCGA GCGAGAGTGC GAGCTGGCAC TGTGGGCCTT CCGGAAGTGG 
AAGTGGTACC AGTTCACATC ATTGCGGGAC CTGCTGTGGG GCAACGCCAT CTTCCTCAAG 
GAGGCCAATG CCATCAGCGT GGAGCTCAAG AAGAAGGTCC AGTTCCAGTT TGTCCTCCTC 
ACGGACACAC TCTACTCCCC GCTGCCACCT GACCTGCTGC CCCCGGAGGC CGCCAAAGAC 
CGAGAGACTC GCCCCTTCCC GCGCACCATC GTGGCCGTGG AGGTCCAAGA CCAGAAGAAC 
GGGGCCACCC ACTACTGGAC GCTGGAGAAA CTCAGGCAGC GGCTAGACCT GATGCGGGAG 
ATGTATGACC GGGCGGCCGA GGTGCCCTCC AGCATCATCG AGGACTGTGA CAACGTGGTG 
ACCGGAGGAG ACCCCTTCTA CGACCGCTTC CCCTGGTTCC GGCTGGTGGG CAGTTCAGTC 
GTCTCTGGCT GCAACAGCTA TCCTCTTCTC AACACATGCA TGAGCGAGCG CATGGCTGCT 
CTCACCCCCT CCCCCACCTT CTCGAGCCCC GACTCCGACG CCACCGAGCC TGCCGAGGAG 
CAGAGCGTGG GGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGACCTGCAG 
GACGACGTCT TTCCGGAGCA CGTGCTGTGC GACGGCCGGG ACCCGTTTTA CGACCGGCCC 
CCCCTGTTCA GTTTAGTAGG AAGGGCCTTC GTGTACCTCA GCAACCTGCT GTACCCCGTG 
CCGCTGGTGC ACCGCGTGGC CGTCGTCAGC GAGAAGGGCG AGGTGAAGGG CTTCCTGCGC 
GTGGCCGTGC AGGCCATCTC AGCCGACGAG GAGGCCCCTG ACTACGGCTC CGGCGTCCGC 
CAGTCGGGAA CAGCCAAAAT CTCCTTTGAC GACCAGCATT TTGAGAAGTT CCAGGCCGAG 
TCCTGCCCCG GGGTGGGCAT GTCCCGCTCA GGGACCTCCC AGGAGGAGCT GCGGATTGTG 
GAAGGCCAGG GCCAGGCCGC AGACTCCGGG CCCTCAGCCG ACGAGGTCAA CAACAACACC 
TGTTCAGCGG TGACCCCGGA AGGCCTCCTG GACAGCCCTG AGAAAGCCGC CCTGGATGGG 
CCCCTGGACG CCGCCCTGGA CCACCTCGGC CTGGGCAGCA CCTTCACCTT CCGCGTGACC 
GTCCTGCAGG CGTCCAGCAT ATCCGCTGAA TATGCCGACA TCTTCTGCCA GTTCAACTTC 
ATCCATCGCC ATGACGAGGC CTTCTCTACG GAGCCCCTGA AGAACACGGG GAGGGGACCC 
CCGCTCGGCT TCTACCACGT CCAAAACATT GCGGTGGAGG TGACCAGGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCCT CGTGTTCGAG GTCTTCGGGC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGACGT GCTCAGCCCC CTGAGGCCCT CTCGCCGCCA CTTCCCACGG 
GTCATGCCGC TGTCCAAGCC AGTGCCGGCC ACCAAGCTCA GCACGCTGAC GCGACCCTGC 
CCGGGCCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TCGAGATCTG CGAGCTGGAG 
GCCAACGGCG ACTACATCCC CGCAGTGGTG GATCACCGCG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TCCACCAGGG CATCCAGAGA CGGATCACCG TGACCCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTGCGG GAGCTGGTGG TGGGTCGCAT CCGAAACACC 
CCGGAAACGG ACGAGTCCCT GATTGACCCT AATATCTTGT CGCTCAGCAT CCTCTCTTCT 
GGATACATCT GCCCAGCCCA GGATGACCGG CAGTTTCTTG ATTCGGACAT GCCTAGCGTT 
TCGTTTGGAA GTGACACTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803926.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802228.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

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CloneID	OBa51458
Clone ID Related Accession (Same CDS sequence)	XM_015462886.1 , XM_010803926.2
Accession Version	XM_010803926.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA ACGTGTCCGA CCTTGAGAAC 
AATAACCGTA GCCGTGGCGG GGTGGAGCTG AACCCAGCCC CTGACAATCT CTCCACAGTG 
ACCAGCGCCC TGGTGGGCAT GAGCCCCTCG TCCTCACTCT CGGCCCTGTC CAGCCGTGCC 
GCCTCCGTGT CCAGCCTGCA CGAGCGCATC TTGTTTGCCC CGGGCAGCGA GGAGGCCATC 
GAAAGGCTGA AGGAGACAGA AAAGATCATC GCTGAGCTCA ACGAGACCTG GGAGGAGAAG 
CTGCGGCGGA CGGAAGCCAT CCGGATGGAG AGGGAAGCAC TGCTGGCCGA GATGGGCGTG 
GCCATGCGGG AGGACGGTGG CACGCTGGGG GTGTTCTCTC CCAAAAAGAC GCCACATCTG 
GTCAACCTGA ATGAGGACCC GCTCATGTCC GAGTGCCTGC TCTACTACAT CAAGGATGGG 
ATCACCAGGG TGGGCCGGGA GGACGCGGAG AAGCGGCAGG ACATTGTTCT GAGCGGGCAC 
TTCATCAAGG AGGAGCACTG TGTCTTCCGG AGCGACTCAT GTGGGGGAAG TGAAGCTGTG 
GTGACCCTAG AGCCCTGTGA AGGGGCGGAC ACCTATGTCA ATGGCAAGAA AGTCACGGAG 
CCCAGCGTCC TGCGGTCAGG AAACCGCATC ATCATGGGCA AGAGCCACGT GTTCCGGTTC 
AACCACCCTG AGCAGGCGCG TCAGGAGCGC GAGCGCACGC CCTGCGCAGA GACGCCGGCC 
GAGCCCGTGG ACTGGGCCTT CGCCCAGCGC GAGCTGCTGG AGAAGCAGGG CATTGACATG 
AAGCAGGAGA TGGAGCAGAG GCTCCAGGAG CTGGAGGACC AGTACCGCCG GGAGCGCGAG 
GAGGCCACCT ACCTGCTGGA GCAGCAGCGG CTGGACTACG AGAGCAAACT GGAGGCCCTG 
CAGAAGCAGA TGGACTCCAG GTACTATCCC GAGGTGAACG AGGAGGAGGA GGAGCCGGAG 
GACGAAGTCC AGTGGACCGA GCGAGAGTGC GAGCTGGCAC TGTGGGCCTT CCGGAAGTGG 
AAGTGGTACC AGTTCACATC ATTGCGGGAC CTGCTGTGGG GCAACGCCAT CTTCCTCAAG 
GAGGCCAATG CCATCAGCGT GGAGCTCAAG AAGAAGGTCC AGTTCCAGTT TGTCCTCCTC 
ACGGACACAC TCTACTCCCC GCTGCCACCT GACCTGCTGC CCCCGGAGGC CGCCAAAGAC 
CGAGAGACTC GCCCCTTCCC GCGCACCATC GTGGCCGTGG AGGTCCAAGA CCAGAAGAAC 
GGGGCCACCC ACTACTGGAC GCTGGAGAAA CTCAGGCAGC GGCTAGACCT GATGCGGGAG 
ATGTATGACC GGGCGGCCGA GGTGCCCTCC AGCATCATCG AGGACTGTGA CAACGTGGTG 
ACCGGAGGAG ACCCCTTCTA CGACCGCTTC CCCTGGTTCC GGCTGGTGGG CAGTTCAGTC 
GTCTCTGGCT GCAACAGCTA TCCTCTTCTC AACACATGCA TGAGCGAGCG CATGGCTGCT 
CTCACCCCCT CCCCCACCTT CTCGAGCCCC GACTCCGACG CCACCGAGCC TGCCGAGGAG 
CAGAGCGTGG GGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGACCTGCAG 
GACGACGTCT TTCCGGAGCA CGTGCTGTGC GACGGCCGGG ACCCGTTTTA CGACCGGCCC 
CCCCTGTTCA GTTTAGTAGG AAGGGCCTTC GTGTACCTCA GCAACCTGCT GTACCCCGTG 
CCGCTGGTGC ACCGCGTGGC CGTCGTCAGC GAGAAGGGCG AGGTGAAGGG CTTCCTGCGC 
GTGGCCGTGC AGGCCATCTC AGCCGACGAG GAGGCCCCTG ACTACGGCTC CGGCGTCCGC 
CAGTCGGGAA CAGCCAAAAT CTCCTTTGAC GACCAGCATT TTGAGAAGTT CCAGGCCGAG 
TCCTGCCCCG GGGTGGGCAT GTCCCGCTCA GGGACCTCCC AGGAGGAGCT GCGGATTGTG 
GAAGGCCAGG GCCAGGCCGC AGACTCCGGG CCCTCAGCCG ACGAGGTCAA CAACAACACC 
TGTTCAGCGG TGACCCCGGA AGGCCTCCTG GACAGCCCTG AGAAAGCCGC CCTGGATGGG 
CCCCTGGACG CCGCCCTGGA CCACCTCGGC CTGGGCAGCA CCTTCACCTT CCGCGTGACC 
GTCCTGCAGG CGTCCAGCAT ATCCGCTGAA TATGCCGACA TCTTCTGCCA GTTCAACTTC 
ATCCATCGCC ATGACGAGGC CTTCTCTACG GAGCCCCTGA AGAACACGGG GAGGGGACCC 
CCGCTCGGCT TCTACCACGT CCAAAACATT GCGGTGGAGG TGACCAGGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCCT CGTGTTCGAG GTCTTCGGGC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGACGT GCTCAGCCCC CTGAGGCCCT CTCGCCGCCA CTTCCCACGG 
GTCATGCCGC TGTCCAAGCC AGTGCCGGCC ACCAAGCTCA GCACGCTGAC GCGACCCTGC 
CCGGGCCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TCGAGATCTG CGAGCTGGAG 
GCCAACGGCG ACTACATCCC CGCAGTGGTG GATCACCGCG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TCCACCAGGG CATCCAGAGA CGGATCACCG TGACCCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTGCGG GAGCTGGTGG TGGGTCGCAT CCGAAACACC 
CCGGAAACGG ACGAGTCCCT GATTGACCCT AATATCTTGT CGCTCAGCAT CCTCTCTTCT 
GGATACATCT GCCCAGCCCA GGATGACCGG CAGTTTCTTG ATTCGGACAT GCCTAGCGTT 
TCGTTTGGAA GTGACACTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802228.1
CDS	323..5791
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDNVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRQFLDSDMPSVSFGSDTRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803926.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803926.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA ACGTGTCCGA CCTTGAGAAC 
AATAACCGTA GCCGTGGCGG GGTGGAGCTG AACCCAGCCC CTGACAATCT CTCCACAGTG 
ACCAGCGCCC TGGTGGGCAT GAGCCCCTCG TCCTCACTCT CGGCCCTGTC CAGCCGTGCC 
GCCTCCGTGT CCAGCCTGCA CGAGCGCATC TTGTTTGCCC CGGGCAGCGA GGAGGCCATC 
GAAAGGCTGA AGGAGACAGA AAAGATCATC GCTGAGCTCA ACGAGACCTG GGAGGAGAAG 
CTGCGGCGGA CGGAAGCCAT CCGGATGGAG AGGGAAGCAC TGCTGGCCGA GATGGGCGTG 
GCCATGCGGG AGGACGGTGG CACGCTGGGG GTGTTCTCTC CCAAAAAGAC GCCACATCTG 
GTCAACCTGA ATGAGGACCC GCTCATGTCC GAGTGCCTGC TCTACTACAT CAAGGATGGG 
ATCACCAGGG TGGGCCGGGA GGACGCGGAG AAGCGGCAGG ACATTGTTCT GAGCGGGCAC 
TTCATCAAGG AGGAGCACTG TGTCTTCCGG AGCGACTCAT GTGGGGGAAG TGAAGCTGTG 
GTGACCCTAG AGCCCTGTGA AGGGGCGGAC ACCTATGTCA ATGGCAAGAA AGTCACGGAG 
CCCAGCGTCC TGCGGTCAGG AAACCGCATC ATCATGGGCA AGAGCCACGT GTTCCGGTTC 
AACCACCCTG AGCAGGCGCG TCAGGAGCGC GAGCGCACGC CCTGCGCAGA GACGCCGGCC 
GAGCCCGTGG ACTGGGCCTT CGCCCAGCGC GAGCTGCTGG AGAAGCAGGG CATTGACATG 
AAGCAGGAGA TGGAGCAGAG GCTCCAGGAG CTGGAGGACC AGTACCGCCG GGAGCGCGAG 
GAGGCCACCT ACCTGCTGGA GCAGCAGCGG CTGGACTACG AGAGCAAACT GGAGGCCCTG 
CAGAAGCAGA TGGACTCCAG GTACTATCCC GAGGTGAACG AGGAGGAGGA GGAGCCGGAG 
GACGAAGTCC AGTGGACCGA GCGAGAGTGC GAGCTGGCAC TGTGGGCCTT CCGGAAGTGG 
AAGTGGTACC AGTTCACATC ATTGCGGGAC CTGCTGTGGG GCAACGCCAT CTTCCTCAAG 
GAGGCCAATG CCATCAGCGT GGAGCTCAAG AAGAAGGTCC AGTTCCAGTT TGTCCTCCTC 
ACGGACACAC TCTACTCCCC GCTGCCACCT GACCTGCTGC CCCCGGAGGC CGCCAAAGAC 
CGAGAGACTC GCCCCTTCCC GCGCACCATC GTGGCCGTGG AGGTCCAAGA CCAGAAGAAC 
GGGGCCACCC ACTACTGGAC GCTGGAGAAA CTCAGGCAGC GGCTAGACCT GATGCGGGAG 
ATGTATGACC GGGCGGCCGA GGTGCCCTCC AGCATCATCG AGGACTGTGA CAACGTGGTG 
ACCGGAGGAG ACCCCTTCTA CGACCGCTTC CCCTGGTTCC GGCTGGTGGG CAGTTCAGTC 
GTCTCTGGCT GCAACAGCTA TCCTCTTCTC AACACATGCA TGAGCGAGCG CATGGCTGCT 
CTCACCCCCT CCCCCACCTT CTCGAGCCCC GACTCCGACG CCACCGAGCC TGCCGAGGAG 
CAGAGCGTGG GGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGGA GGACCTGCAG 
GACGACGTCT TTCCGGAGCA CGTGCTGTGC GACGGCCGGG ACCCGTTTTA CGACCGGCCC 
CCCCTGTTCA GTTTAGTAGG AAGGGCCTTC GTGTACCTCA GCAACCTGCT GTACCCCGTG 
CCGCTGGTGC ACCGCGTGGC CGTCGTCAGC GAGAAGGGCG AGGTGAAGGG CTTCCTGCGC 
GTGGCCGTGC AGGCCATCTC AGCCGACGAG GAGGCCCCTG ACTACGGCTC CGGCGTCCGC 
CAGTCGGGAA CAGCCAAAAT CTCCTTTGAC GACCAGCATT TTGAGAAGTT CCAGGCCGAG 
TCCTGCCCCG GGGTGGGCAT GTCCCGCTCA GGGACCTCCC AGGAGGAGCT GCGGATTGTG 
GAAGGCCAGG GCCAGGCCGC AGACTCCGGG CCCTCAGCCG ACGAGGTCAA CAACAACACC 
TGTTCAGCGG TGACCCCGGA AGGCCTCCTG GACAGCCCTG AGAAAGCCGC CCTGGATGGG 
CCCCTGGACG CCGCCCTGGA CCACCTCGGC CTGGGCAGCA CCTTCACCTT CCGCGTGACC 
GTCCTGCAGG CGTCCAGCAT ATCCGCTGAA TATGCCGACA TCTTCTGCCA GTTCAACTTC 
ATCCATCGCC ATGACGAGGC CTTCTCTACG GAGCCCCTGA AGAACACGGG GAGGGGACCC 
CCGCTCGGCT TCTACCACGT CCAAAACATT GCGGTGGAGG TGACCAGGTC CTTCATCGAG 
TACATCAAGA GCCAGCCCCT CGTGTTCGAG GTCTTCGGGC ACTACCAGCA GCACCCGTTC 
CCGCCCCTCT GCAAGGACGT GCTCAGCCCC CTGAGGCCCT CTCGCCGCCA CTTCCCACGG 
GTCATGCCGC TGTCCAAGCC AGTGCCGGCC ACCAAGCTCA GCACGCTGAC GCGACCCTGC 
CCGGGCCCCT GCCACTGCAA GTACGACCTG CTGGTCTACT TCGAGATCTG CGAGCTGGAG 
GCCAACGGCG ACTACATCCC CGCAGTGGTG GATCACCGCG GCGGGATGCC GTGCATGGGG 
ACCTTCCTGC TCCACCAGGG CATCCAGAGA CGGATCACCG TGACCCTGCT GCACGAGACG 
GGCAGCCACA TCCGCTGGAA GGAAGTGCGG GAGCTGGTGG TGGGTCGCAT CCGAAACACC 
CCGGAAACGG ACGAGTCCCT GATTGACCCT AATATCTTGT CGCTCAGCAT CCTCTCTTCT 
GGATACATCT GCCCAGCCCA GGATGACCGG CAGTTTCTTG ATTCGGACAT GCCTAGCGTT 
TCGTTTGGAA GTGACACTAG AACCTTTTAC CAGTTTGAGG CTGCGTGGGA CAGCTCCATG 
CACAACTCCC TCCTGCTGAA CCGGGTCACC CCTTACCGAG AGAAGATTTA CATGACCCTC 
TCTGCTTATA TCGAGATGGA GAACTGCACG CAGCCGGCCG TGGTCACCAA AGACTTCTGC 
ATGGTCTTCT ACTCGCGCGA CGCCAAGCTG CCCGCCTCGC GCTCCATCCG CAACCTGTTC 
GGCAGTGGGA GCCTGCGGGC CTCAGAGAGC AACCGCGTGA CGGGCGTGTA CGAGCTGAGT 
CTGTGCCACG TGGCTGACGC GGGCAGCCCA GGCATGCAGC GGCGGCGCCG GCGGGTGCTG 
GACACATCCG TGGCCTACGT CCGCGGCGAG GAGAACCTGG CGGGCTGGAG GCCGCGTAGT 
GACAGCCTCA TCCTGGACCA CCAGTGGGAG CTGGAGAAGC TGAGTCTCCT GCAGGAGGTG 
GAGAAGACCA GGCACTACCT GTTGCTGCGA GAGAAGCTGG AGGCGGCCCA GCGGCCCGGC 
CCCGAGACGC TGTCCCCCGC CGCCAGTGAC GGCTCCGAGG CCCACGGCTC CTCCAGTGCC 
TCCTCCCCGC TCACGGCTGA GGCCCGGCCA GCATCCCTGG AGGCGCCCAG CGAGCGGCAG 
CGGGAGCTGG CTGTCAAGTG CTTACGGCTC CTCACCCACT CCTTCAACAG AGAGTACACC 
CACAGCCACG TCTGCGTCAG CGCCAGCGAG AGCAAGCTCT CTGAGATGTC GGTCACCCTG 
CTGCGGGACC CGTCCATGTC CCCGCTGGGG GCCGCCACGC TCACTCCCTC CTCTACCTGC 
CCCTCCCTGG TCGAGGGCCG GTATGGGGCC ACCGACCTGA GGACCCCACA GCCCTGCTCC 
CGGCCCGCCA GCCCGGAGCC TGAGCCCCTG CCCGAGGTAG ATGCCAAGAA GCTCCCCTCC 
CCTGCCCGGG CAGCGGAAGC CGACAAGGAG CCCCAGCGCC TGCTGGTGCC TGACATCCAG 
GAAATCCGTG TCAGCCCCAT CGTCTCCAAG AAGGGCTACC TGCACTTCCT GGAGCCGCAC 
ACGGCCGGCT GGGCCAAGCG CTTCGTGGTG GTGCGGCGGC CCTACGCCTA CATGTACAAC 
CACGACAAGG ACGCCGTGGA GCGCTTCGTG CTCAACCTGT CCACCGCCCA GGTGGAGTAC 
AGCGAGGACC AGCAGGCGAT GCTCAAGACC CCCAACACGT TCGCGGTGTG CACGGAGCAC 
CGGGGCATCT TGCTGCAGGC CAGCAGTGAC AAGGACATGC ACGACTGGCT GTACGCCTTC 
AACCCCCTGC TGGCCGGGAC CATCCGGTCC AAGCTGTCCA GAAGGAGGTC TGCCCAGATG 
CGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462887.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318373.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

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CloneID	OBa51459
Clone ID Related Accession (Same CDS sequence)	XM_015462887.1 , XM_010803927.2
Accession Version	XM_015462887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGAACC 
TTTTACCAGT TTGAGGCTGC GTGGGACAGC TCCATGCACA ACTCCCTCCT GCTGAACCGG 
GTCACCCCTT ACCGAGAGAA GATTTACATG ACCCTCTCTG CTTATATCGA GATGGAGAAC 
TGCACGCAGC CGGCCGTGGT CACCAAAGAC TTCTGCATGG TCTTCTACTC GCGCGACGCC 
AAGCTGCCCG CCTCGCGCTC CATCCGCAAC CTGTTCGGCA GTGGGAGCCT GCGGGCCTCA 
GAGAGCAACC GCGTGACGGG CGTGTACGAG CTGAGTCTGT GCCACGTGGC TGACGCGGGC 
AGCCCAGGCA TGCAGCGGCG GCGCCGGCGG GTGCTGGACA CATCCGTGGC CTACGTCCGC 
GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG CGTAGTGACA GCCTCATCCT GGACCACCAG 
TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG GAGGTGGAGA AGACCAGGCA CTACCTGTTG 
CTGCGAGAGA AGCTGGAGGC GGCCCAGCGG CCCGGCCCCG AGACGCTGTC CCCCGCCGCC 
AGTGACGGCT CCGAGGCCCA CGGCTCCTCC AGTGCCTCCT CCCCGCTCAC GGCTGAGGCC 
CGGCCAGCAT CCCTGGAGGC GCCCAGCGAG CGGCAGCGGG AGCTGGCTGT CAAGTGCTTA 
CGGCTCCTCA CCCACTCCTT CAACAGAGAG TACACCCACA GCCACGTCTG CGTCAGCGCC 
AGCGAGAGCA AGCTCTCTGA GATGTCGGTC ACCCTGCTGC GGGACCCGTC CATGTCCCCG 
CTGGGGGCCG CCACGCTCAC TCCCTCCTCT ACCTGCCCCT CCCTGGTCGA GGGCCGGTAT 
GGGGCCACCG ACCTGAGGAC CCCACAGCCC TGCTCCCGGC CCGCCAGCCC GGAGCCTGAG 
CCCCTGCCCG AGGTAGATGC CAAGAAGCTC CCCTCCCCTG CCCGGGCAGC GGAAGCCGAC 
AAGGAGCCCC AGCGCCTGCT GGTGCCTGAC ATCCAGGAAA TCCGTGTCAG CCCCATCGTC 
TCCAAGAAGG GCTACCTGCA CTTCCTGGAG CCGCACACGG CCGGCTGGGC CAAGCGCTTC 
GTGGTGGTGC GGCGGCCCTA CGCCTACATG TACAACCACG ACAAGGACGC CGTGGAGCGC 
TTCGTGCTCA ACCTGTCCAC CGCCCAGGTG GAGTACAGCG AGGACCAGCA GGCGATGCTC 
AAGACCCCCA ACACGTTCGC GGTGTGCACG GAGCACCGGG GCATCTTGCT GCAGGCCAGC 
AGTGACAAGG ACATGCACGA CTGGCTGTAC GCCTTCAACC CCCTGCTGGC CGGGACCATC 
CGGTCCAAGC TGTCCAGAAG GAGGTCTGCC CAGATGCGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318373.1
CDS	323..5767
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462887.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462887.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGAACC 
TTTTACCAGT TTGAGGCTGC GTGGGACAGC TCCATGCACA ACTCCCTCCT GCTGAACCGG 
GTCACCCCTT ACCGAGAGAA GATTTACATG ACCCTCTCTG CTTATATCGA GATGGAGAAC 
TGCACGCAGC CGGCCGTGGT CACCAAAGAC TTCTGCATGG TCTTCTACTC GCGCGACGCC 
AAGCTGCCCG CCTCGCGCTC CATCCGCAAC CTGTTCGGCA GTGGGAGCCT GCGGGCCTCA 
GAGAGCAACC GCGTGACGGG CGTGTACGAG CTGAGTCTGT GCCACGTGGC TGACGCGGGC 
AGCCCAGGCA TGCAGCGGCG GCGCCGGCGG GTGCTGGACA CATCCGTGGC CTACGTCCGC 
GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG CGTAGTGACA GCCTCATCCT GGACCACCAG 
TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG GAGGTGGAGA AGACCAGGCA CTACCTGTTG 
CTGCGAGAGA AGCTGGAGGC GGCCCAGCGG CCCGGCCCCG AGACGCTGTC CCCCGCCGCC 
AGTGACGGCT CCGAGGCCCA CGGCTCCTCC AGTGCCTCCT CCCCGCTCAC GGCTGAGGCC 
CGGCCAGCAT CCCTGGAGGC GCCCAGCGAG CGGCAGCGGG AGCTGGCTGT CAAGTGCTTA 
CGGCTCCTCA CCCACTCCTT CAACAGAGAG TACACCCACA GCCACGTCTG CGTCAGCGCC 
AGCGAGAGCA AGCTCTCTGA GATGTCGGTC ACCCTGCTGC GGGACCCGTC CATGTCCCCG 
CTGGGGGCCG CCACGCTCAC TCCCTCCTCT ACCTGCCCCT CCCTGGTCGA GGGCCGGTAT 
GGGGCCACCG ACCTGAGGAC CCCACAGCCC TGCTCCCGGC CCGCCAGCCC GGAGCCTGAG 
CCCCTGCCCG AGGTAGATGC CAAGAAGCTC CCCTCCCCTG CCCGGGCAGC GGAAGCCGAC 
AAGGAGCCCC AGCGCCTGCT GGTGCCTGAC ATCCAGGAAA TCCGTGTCAG CCCCATCGTC 
TCCAAGAAGG GCTACCTGCA CTTCCTGGAG CCGCACACGG CCGGCTGGGC CAAGCGCTTC 
GTGGTGGTGC GGCGGCCCTA CGCCTACATG TACAACCACG ACAAGGACGC CGTGGAGCGC 
TTCGTGCTCA ACCTGTCCAC CGCCCAGGTG GAGTACAGCG AGGACCAGCA GGCGATGCTC 
AAGACCCCCA ACACGTTCGC GGTGTGCACG GAGCACCGGG GCATCTTGCT GCAGGCCAGC 
AGTGACAAGG ACATGCACGA CTGGCTGTAC GCCTTCAACC CCCTGCTGGC CGGGACCATC 
CGGTCCAAGC TGTCCAGAAG GAGGTCTGCC CAGATGCGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803927.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802229.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

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CloneID	OBa51459
Clone ID Related Accession (Same CDS sequence)	XM_015462887.1 , XM_010803927.2
Accession Version	XM_010803927.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5445bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGAACC 
TTTTACCAGT TTGAGGCTGC GTGGGACAGC TCCATGCACA ACTCCCTCCT GCTGAACCGG 
GTCACCCCTT ACCGAGAGAA GATTTACATG ACCCTCTCTG CTTATATCGA GATGGAGAAC 
TGCACGCAGC CGGCCGTGGT CACCAAAGAC TTCTGCATGG TCTTCTACTC GCGCGACGCC 
AAGCTGCCCG CCTCGCGCTC CATCCGCAAC CTGTTCGGCA GTGGGAGCCT GCGGGCCTCA 
GAGAGCAACC GCGTGACGGG CGTGTACGAG CTGAGTCTGT GCCACGTGGC TGACGCGGGC 
AGCCCAGGCA TGCAGCGGCG GCGCCGGCGG GTGCTGGACA CATCCGTGGC CTACGTCCGC 
GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG CGTAGTGACA GCCTCATCCT GGACCACCAG 
TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG GAGGTGGAGA AGACCAGGCA CTACCTGTTG 
CTGCGAGAGA AGCTGGAGGC GGCCCAGCGG CCCGGCCCCG AGACGCTGTC CCCCGCCGCC 
AGTGACGGCT CCGAGGCCCA CGGCTCCTCC AGTGCCTCCT CCCCGCTCAC GGCTGAGGCC 
CGGCCAGCAT CCCTGGAGGC GCCCAGCGAG CGGCAGCGGG AGCTGGCTGT CAAGTGCTTA 
CGGCTCCTCA CCCACTCCTT CAACAGAGAG TACACCCACA GCCACGTCTG CGTCAGCGCC 
AGCGAGAGCA AGCTCTCTGA GATGTCGGTC ACCCTGCTGC GGGACCCGTC CATGTCCCCG 
CTGGGGGCCG CCACGCTCAC TCCCTCCTCT ACCTGCCCCT CCCTGGTCGA GGGCCGGTAT 
GGGGCCACCG ACCTGAGGAC CCCACAGCCC TGCTCCCGGC CCGCCAGCCC GGAGCCTGAG 
CCCCTGCCCG AGGTAGATGC CAAGAAGCTC CCCTCCCCTG CCCGGGCAGC GGAAGCCGAC 
AAGGAGCCCC AGCGCCTGCT GGTGCCTGAC ATCCAGGAAA TCCGTGTCAG CCCCATCGTC 
TCCAAGAAGG GCTACCTGCA CTTCCTGGAG CCGCACACGG CCGGCTGGGC CAAGCGCTTC 
GTGGTGGTGC GGCGGCCCTA CGCCTACATG TACAACCACG ACAAGGACGC CGTGGAGCGC 
TTCGTGCTCA ACCTGTCCAC CGCCCAGGTG GAGTACAGCG AGGACCAGCA GGCGATGCTC 
AAGACCCCCA ACACGTTCGC GGTGTGCACG GAGCACCGGG GCATCTTGCT GCAGGCCAGC 
AGTGACAAGG ACATGCACGA CTGGCTGTAC GCCTTCAACC CCCTGCTGGC CGGGACCATC 
CGGTCCAAGC TGTCCAGAAG GAGGTCTGCC CAGATGCGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802229.1
CDS	323..5767
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDTGTVPGGPRYVSDLENNNRSRGGVELNPAPDNLSTVTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803927.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803927.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA CCGGCACTGT GCCCGGAGGA 
CCCAGATACG TGTCCGACCT TGAGAACAAT AACCGTAGCC GTGGCGGGGT GGAGCTGAAC 
CCAGCCCCTG ACAATCTCTC CACAGTGACC AGCGCCCTGG TGGGCATGAG CCCCTCGTCC 
TCACTCTCGG CCCTGTCCAG CCGTGCCGCC TCCGTGTCCA GCCTGCACGA GCGCATCTTG 
TTTGCCCCGG GCAGCGAGGA GGCCATCGAA AGGCTGAAGG AGACAGAAAA GATCATCGCT 
GAGCTCAACG AGACCTGGGA GGAGAAGCTG CGGCGGACGG AAGCCATCCG GATGGAGAGG 
GAAGCACTGC TGGCCGAGAT GGGCGTGGCC ATGCGGGAGG ACGGTGGCAC GCTGGGGGTG 
TTCTCTCCCA AAAAGACGCC ACATCTGGTC AACCTGAATG AGGACCCGCT CATGTCCGAG 
TGCCTGCTCT ACTACATCAA GGATGGGATC ACCAGGGTGG GCCGGGAGGA CGCGGAGAAG 
CGGCAGGACA TTGTTCTGAG CGGGCACTTC ATCAAGGAGG AGCACTGTGT CTTCCGGAGC 
GACTCATGTG GGGGAAGTGA AGCTGTGGTG ACCCTAGAGC CCTGTGAAGG GGCGGACACC 
TATGTCAATG GCAAGAAAGT CACGGAGCCC AGCGTCCTGC GGTCAGGAAA CCGCATCATC 
ATGGGCAAGA GCCACGTGTT CCGGTTCAAC CACCCTGAGC AGGCGCGTCA GGAGCGCGAG 
CGCACGCCCT GCGCAGAGAC GCCGGCCGAG CCCGTGGACT GGGCCTTCGC CCAGCGCGAG 
CTGCTGGAGA AGCAGGGCAT TGACATGAAG CAGGAGATGG AGCAGAGGCT CCAGGAGCTG 
GAGGACCAGT ACCGCCGGGA GCGCGAGGAG GCCACCTACC TGCTGGAGCA GCAGCGGCTG 
GACTACGAGA GCAAACTGGA GGCCCTGCAG AAGCAGATGG ACTCCAGGTA CTATCCCGAG 
GTGAACGAGG AGGAGGAGGA GCCGGAGGAC GAAGTCCAGT GGACCGAGCG AGAGTGCGAG 
CTGGCACTGT GGGCCTTCCG GAAGTGGAAG TGGTACCAGT TCACATCATT GCGGGACCTG 
CTGTGGGGCA ACGCCATCTT CCTCAAGGAG GCCAATGCCA TCAGCGTGGA GCTCAAGAAG 
AAGGTCCAGT TCCAGTTTGT CCTCCTCACG GACACACTCT ACTCCCCGCT GCCACCTGAC 
CTGCTGCCCC CGGAGGCCGC CAAAGACCGA GAGACTCGCC CCTTCCCGCG CACCATCGTG 
GCCGTGGAGG TCCAAGACCA GAAGAACGGG GCCACCCACT ACTGGACGCT GGAGAAACTC 
AGGCAGCGGC TAGACCTGAT GCGGGAGATG TATGACCGGG CGGCCGAGGT GCCCTCCAGC 
ATCATCGAGG ACTGTGACAA CGTGGTGACC GGAGGAGACC CCTTCTACGA CCGCTTCCCC 
TGGTTCCGGC TGGTGGGCAG TTCAGTCGTC TCTGGCTGCA ACAGCTATCC TCTTCTCAAC 
ACATGCATGA GCGAGCGCAT GGCTGCTCTC ACCCCCTCCC CCACCTTCTC GAGCCCCGAC 
TCCGACGCCA CCGAGCCTGC CGAGGAGCAG AGCGTGGGGG AGGAGGAGGA GGAGGAGGAG 
GAGGAGGAGG AGGAGGAGGA CCTGCAGGAC GACGTCTTTC CGGAGCACGT GCTGTGCGAC 
GGCCGGGACC CGTTTTACGA CCGGCCCCCC CTGTTCAGTT TAGTAGGAAG GGCCTTCGTG 
TACCTCAGCA ACCTGCTGTA CCCCGTGCCG CTGGTGCACC GCGTGGCCGT CGTCAGCGAG 
AAGGGCGAGG TGAAGGGCTT CCTGCGCGTG GCCGTGCAGG CCATCTCAGC CGACGAGGAG 
GCCCCTGACT ACGGCTCCGG CGTCCGCCAG TCGGGAACAG CCAAAATCTC CTTTGACGAC 
CAGCATTTTG AGAAGTTCCA GGCCGAGTCC TGCCCCGGGG TGGGCATGTC CCGCTCAGGG 
ACCTCCCAGG AGGAGCTGCG GATTGTGGAA GGCCAGGGCC AGGCCGCAGA CTCCGGGCCC 
TCAGCCGACG AGGTCAACAA CAACACCTGT TCAGCGGTGA CCCCGGAAGG CCTCCTGGAC 
AGCCCTGAGA AAGCCGCCCT GGATGGGCCC CTGGACGCCG CCCTGGACCA CCTCGGCCTG 
GGCAGCACCT TCACCTTCCG CGTGACCGTC CTGCAGGCGT CCAGCATATC CGCTGAATAT 
GCCGACATCT TCTGCCAGTT CAACTTCATC CATCGCCATG ACGAGGCCTT CTCTACGGAG 
CCCCTGAAGA ACACGGGGAG GGGACCCCCG CTCGGCTTCT ACCACGTCCA AAACATTGCG 
GTGGAGGTGA CCAGGTCCTT CATCGAGTAC ATCAAGAGCC AGCCCCTCGT GTTCGAGGTC 
TTCGGGCACT ACCAGCAGCA CCCGTTCCCG CCCCTCTGCA AGGACGTGCT CAGCCCCCTG 
AGGCCCTCTC GCCGCCACTT CCCACGGGTC ATGCCGCTGT CCAAGCCAGT GCCGGCCACC 
AAGCTCAGCA CGCTGACGCG ACCCTGCCCG GGCCCCTGCC ACTGCAAGTA CGACCTGCTG 
GTCTACTTCG AGATCTGCGA GCTGGAGGCC AACGGCGACT ACATCCCCGC AGTGGTGGAT 
CACCGCGGCG GGATGCCGTG CATGGGGACC TTCCTGCTCC ACCAGGGCAT CCAGAGACGG 
ATCACCGTGA CCCTGCTGCA CGAGACGGGC AGCCACATCC GCTGGAAGGA AGTGCGGGAG 
CTGGTGGTGG GTCGCATCCG AAACACCCCG GAAACGGACG AGTCCCTGAT TGACCCTAAT 
ATCTTGTCGC TCAGCATCCT CTCTTCTGGA TACATCTGCC CAGCCCAGGA TGACCGAACC 
TTTTACCAGT TTGAGGCTGC GTGGGACAGC TCCATGCACA ACTCCCTCCT GCTGAACCGG 
GTCACCCCTT ACCGAGAGAA GATTTACATG ACCCTCTCTG CTTATATCGA GATGGAGAAC 
TGCACGCAGC CGGCCGTGGT CACCAAAGAC TTCTGCATGG TCTTCTACTC GCGCGACGCC 
AAGCTGCCCG CCTCGCGCTC CATCCGCAAC CTGTTCGGCA GTGGGAGCCT GCGGGCCTCA 
GAGAGCAACC GCGTGACGGG CGTGTACGAG CTGAGTCTGT GCCACGTGGC TGACGCGGGC 
AGCCCAGGCA TGCAGCGGCG GCGCCGGCGG GTGCTGGACA CATCCGTGGC CTACGTCCGC 
GGCGAGGAGA ACCTGGCGGG CTGGAGGCCG CGTAGTGACA GCCTCATCCT GGACCACCAG 
TGGGAGCTGG AGAAGCTGAG TCTCCTGCAG GAGGTGGAGA AGACCAGGCA CTACCTGTTG 
CTGCGAGAGA AGCTGGAGGC GGCCCAGCGG CCCGGCCCCG AGACGCTGTC CCCCGCCGCC 
AGTGACGGCT CCGAGGCCCA CGGCTCCTCC AGTGCCTCCT CCCCGCTCAC GGCTGAGGCC 
CGGCCAGCAT CCCTGGAGGC GCCCAGCGAG CGGCAGCGGG AGCTGGCTGT CAAGTGCTTA 
CGGCTCCTCA CCCACTCCTT CAACAGAGAG TACACCCACA GCCACGTCTG CGTCAGCGCC 
AGCGAGAGCA AGCTCTCTGA GATGTCGGTC ACCCTGCTGC GGGACCCGTC CATGTCCCCG 
CTGGGGGCCG CCACGCTCAC TCCCTCCTCT ACCTGCCCCT CCCTGGTCGA GGGCCGGTAT 
GGGGCCACCG ACCTGAGGAC CCCACAGCCC TGCTCCCGGC CCGCCAGCCC GGAGCCTGAG 
CCCCTGCCCG AGGTAGATGC CAAGAAGCTC CCCTCCCCTG CCCGGGCAGC GGAAGCCGAC 
AAGGAGCCCC AGCGCCTGCT GGTGCCTGAC ATCCAGGAAA TCCGTGTCAG CCCCATCGTC 
TCCAAGAAGG GCTACCTGCA CTTCCTGGAG CCGCACACGG CCGGCTGGGC CAAGCGCTTC 
GTGGTGGTGC GGCGGCCCTA CGCCTACATG TACAACCACG ACAAGGACGC CGTGGAGCGC 
TTCGTGCTCA ACCTGTCCAC CGCCCAGGTG GAGTACAGCG AGGACCAGCA GGCGATGCTC 
AAGACCCCCA ACACGTTCGC GGTGTGCACG GAGCACCGGG GCATCTTGCT GCAGGCCAGC 
AGTGACAAGG ACATGCACGA CTGGCTGTAC GCCTTCAACC CCCTGCTGGC CGGGACCATC 
CGGTCCAAGC TGTCCAGAAG GAGGTCTGCC CAGATGCGGG TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_015462896.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318382.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

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CloneID	OBa51461
Clone ID Related Accession (Same CDS sequence)	XM_015462896.1 , XM_010803931.2
Accession Version	XM_015462896.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGC AGGACGACGT CTTTCCGGAG 
CACGTGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTGTACCT CAGCAACCTG CTGTACCCCG TGCCGCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG GGCTTCCTGC GCGTGGCCGT GCAGGCCATC 
TCAGCCGACG AGGAGGCCCC TGACTACGGC TCCGGCGTCC GCCAGTCGGG AACAGCCAAA 
ATCTCCTTTG ACGACCAGCA TTTTGAGAAG TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC 
ATGTCCCGCT CAGGGACCTC CCAGGAGGAG CTGCGGATTG TGGAAGGCCA GGGCCAGGCC 
GCAGACTCCG GGCCCTCAGC CGACGAGGTC AACAACAACA CCTGTTCAGC GGTGACCCCG 
GAAGGCCTCC TGGACAGCCC TGAGAAAGCC GCCCTGGATG GGCCCCTGGA CGCCGCCCTG 
GACCACCTCG GCCTGGGCAG CACCTTCACC TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC 
ATATCCGCTG AATATGCCGA CATCTTCTGC CAGTTCAACT TCATCCATCG CCATGACGAG 
GCCTTCTCTA CGGAGCCCCT GAAGAACACG GGGAGGGGAC CCCCGCTCGG CTTCTACCAC 
GTCCAAAACA TTGCGGTGGA GGTGACCAGG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
CTCGTGTTCG AGGTCTTCGG GCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAC 
GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC CACTTCCCAC GGGTCATGCC GCTGTCCAAG 
CCAGTGCCGG CCACCAAGCT CAGCACGCTG ACGCGACCCT GCCCGGGCCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTCGAGATC TGCGAGCTGG AGGCCAACGG CGACTACATC 
CCCGCAGTGG TGGATCACCG CGGCGGGATG CCGTGCATGG GGACCTTCCT GCTCCACCAG 
GGCATCCAGA GACGGATCAC CGTGACCCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC ATCCGAAACA CCCCGGAAAC GGACGAGTCC 
CTGATTGACC CTAATATCTT GTCGCTCAGC ATCCTCTCTT CTGGATACAT CTGCCCAGCC 
CAGGATGACC GAACCTTTTA CCAGTTTGAG GCTGCGTGGG ACAGCTCCAT GCACAACTCC 
CTCCTGCTGA ACCGGGTCAC CCCTTACCGA GAGAAGATTT ACATGACCCT CTCTGCTTAT 
ATCGAGATGG AGAACTGCAC GCAGCCGGCC GTGGTCACCA AAGACTTCTG CATGGTCTTC 
TACTCGCGCG ACGCCAAGCT GCCCGCCTCG CGCTCCATCC GCAACCTGTT CGGCAGTGGG 
AGCCTGCGGG CCTCAGAGAG CAACCGCGTG ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC 
GTGGCTGACG CGGGCAGCCC AGGCATGCAG CGGCGGCGCC GGCGGGTGCT GGACACATCC 
GTGGCCTACG TCCGCGGCGA GGAGAACCTG GCGGGCTGGA GGCCGCGTAG TGACAGCCTC 
ATCCTGGACC ACCAGTGGGA GCTGGAGAAG CTGAGTCTCC TGCAGGAGGT GGAGAAGACC 
AGGCACTACC TGTTGCTGCG AGAGAAGCTG GAGGCGGCCC AGCGGCCCGG CCCCGAGACG 
CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG GCCCACGGCT CCTCCAGTGC CTCCTCCCCG 
CTCACGGCTG AGGCCCGGCC AGCATCCCTG GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG 
GCTGTCAAGT GCTTACGGCT CCTCACCCAC TCCTTCAACA GAGAGTACAC CCACAGCCAC 
GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC TCTGAGATGT CGGTCACCCT GCTGCGGGAC 
CCGTCCATGT CCCCGCTGGG GGCCGCCACG CTCACTCCCT CCTCTACCTG CCCCTCCCTG 
GTCGAGGGCC GGTATGGGGC CACCGACCTG AGGACCCCAC AGCCCTGCTC CCGGCCCGCC 
AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA GATGCCAAGA AGCTCCCCTC CCCTGCCCGG 
GCAGCGGAAG CCGACAAGGA GCCCCAGCGC CTGCTGGTGC CTGACATCCA GGAAATCCGT 
GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC CTGCACTTCC TGGAGCCGCA CACGGCCGGC 
TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG CCCTACGCCT ACATGTACAA CCACGACAAG 
GACGCCGTGG AGCGCTTCGT GCTCAACCTG TCCACCGCCC AGGTGGAGTA CAGCGAGGAC 
CAGCAGGCGA TGCTCAAGAC CCCCAACACG TTCGCGGTGT GCACGGAGCA CCGGGGCATC 
TTGCTGCAGG CCAGCAGTGA CAAGGACATG CACGACTGGC TGTACGCCTT CAACCCCCTG 
CTGGCCGGGA CCATCCGGTC CAAGCTGTCC AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318382.1
CDS	323..5662
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_015462896.1
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015462896.1

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGC AGGACGACGT CTTTCCGGAG 
CACGTGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTGTACCT CAGCAACCTG CTGTACCCCG TGCCGCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG GGCTTCCTGC GCGTGGCCGT GCAGGCCATC 
TCAGCCGACG AGGAGGCCCC TGACTACGGC TCCGGCGTCC GCCAGTCGGG AACAGCCAAA 
ATCTCCTTTG ACGACCAGCA TTTTGAGAAG TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC 
ATGTCCCGCT CAGGGACCTC CCAGGAGGAG CTGCGGATTG TGGAAGGCCA GGGCCAGGCC 
GCAGACTCCG GGCCCTCAGC CGACGAGGTC AACAACAACA CCTGTTCAGC GGTGACCCCG 
GAAGGCCTCC TGGACAGCCC TGAGAAAGCC GCCCTGGATG GGCCCCTGGA CGCCGCCCTG 
GACCACCTCG GCCTGGGCAG CACCTTCACC TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC 
ATATCCGCTG AATATGCCGA CATCTTCTGC CAGTTCAACT TCATCCATCG CCATGACGAG 
GCCTTCTCTA CGGAGCCCCT GAAGAACACG GGGAGGGGAC CCCCGCTCGG CTTCTACCAC 
GTCCAAAACA TTGCGGTGGA GGTGACCAGG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
CTCGTGTTCG AGGTCTTCGG GCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAC 
GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC CACTTCCCAC GGGTCATGCC GCTGTCCAAG 
CCAGTGCCGG CCACCAAGCT CAGCACGCTG ACGCGACCCT GCCCGGGCCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTCGAGATC TGCGAGCTGG AGGCCAACGG CGACTACATC 
CCCGCAGTGG TGGATCACCG CGGCGGGATG CCGTGCATGG GGACCTTCCT GCTCCACCAG 
GGCATCCAGA GACGGATCAC CGTGACCCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC ATCCGAAACA CCCCGGAAAC GGACGAGTCC 
CTGATTGACC CTAATATCTT GTCGCTCAGC ATCCTCTCTT CTGGATACAT CTGCCCAGCC 
CAGGATGACC GAACCTTTTA CCAGTTTGAG GCTGCGTGGG ACAGCTCCAT GCACAACTCC 
CTCCTGCTGA ACCGGGTCAC CCCTTACCGA GAGAAGATTT ACATGACCCT CTCTGCTTAT 
ATCGAGATGG AGAACTGCAC GCAGCCGGCC GTGGTCACCA AAGACTTCTG CATGGTCTTC 
TACTCGCGCG ACGCCAAGCT GCCCGCCTCG CGCTCCATCC GCAACCTGTT CGGCAGTGGG 
AGCCTGCGGG CCTCAGAGAG CAACCGCGTG ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC 
GTGGCTGACG CGGGCAGCCC AGGCATGCAG CGGCGGCGCC GGCGGGTGCT GGACACATCC 
GTGGCCTACG TCCGCGGCGA GGAGAACCTG GCGGGCTGGA GGCCGCGTAG TGACAGCCTC 
ATCCTGGACC ACCAGTGGGA GCTGGAGAAG CTGAGTCTCC TGCAGGAGGT GGAGAAGACC 
AGGCACTACC TGTTGCTGCG AGAGAAGCTG GAGGCGGCCC AGCGGCCCGG CCCCGAGACG 
CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG GCCCACGGCT CCTCCAGTGC CTCCTCCCCG 
CTCACGGCTG AGGCCCGGCC AGCATCCCTG GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG 
GCTGTCAAGT GCTTACGGCT CCTCACCCAC TCCTTCAACA GAGAGTACAC CCACAGCCAC 
GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC TCTGAGATGT CGGTCACCCT GCTGCGGGAC 
CCGTCCATGT CCCCGCTGGG GGCCGCCACG CTCACTCCCT CCTCTACCTG CCCCTCCCTG 
GTCGAGGGCC GGTATGGGGC CACCGACCTG AGGACCCCAC AGCCCTGCTC CCGGCCCGCC 
AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA GATGCCAAGA AGCTCCCCTC CCCTGCCCGG 
GCAGCGGAAG CCGACAAGGA GCCCCAGCGC CTGCTGGTGC CTGACATCCA GGAAATCCGT 
GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC CTGCACTTCC TGGAGCCGCA CACGGCCGGC 
TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG CCCTACGCCT ACATGTACAA CCACGACAAG 
GACGCCGTGG AGCGCTTCGT GCTCAACCTG TCCACCGCCC AGGTGGAGTA CAGCGAGGAC 
CAGCAGGCGA TGCTCAAGAC CCCCAACACG TTCGCGGTGT GCACGGAGCA CCGGGGCATC 
TTGCTGCAGG CCAGCAGTGA CAAGGACATG CACGACTGGC TGTACGCCTT CAACCCCCTG 
CTGGCCGGGA CCATCCGGTC CAAGCTGTCC AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KIF1A ( XM_010803931.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010802233.1 Bos taurus kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

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CloneID	OBa51461
Clone ID Related Accession (Same CDS sequence)	XM_015462896.1 , XM_010803931.2
Accession Version	XM_010803931.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5340bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	kinesin-like protein KIF1A isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103880.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGC AGGACGACGT CTTTCCGGAG 
CACGTGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTGTACCT CAGCAACCTG CTGTACCCCG TGCCGCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG GGCTTCCTGC GCGTGGCCGT GCAGGCCATC 
TCAGCCGACG AGGAGGCCCC TGACTACGGC TCCGGCGTCC GCCAGTCGGG AACAGCCAAA 
ATCTCCTTTG ACGACCAGCA TTTTGAGAAG TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC 
ATGTCCCGCT CAGGGACCTC CCAGGAGGAG CTGCGGATTG TGGAAGGCCA GGGCCAGGCC 
GCAGACTCCG GGCCCTCAGC CGACGAGGTC AACAACAACA CCTGTTCAGC GGTGACCCCG 
GAAGGCCTCC TGGACAGCCC TGAGAAAGCC GCCCTGGATG GGCCCCTGGA CGCCGCCCTG 
GACCACCTCG GCCTGGGCAG CACCTTCACC TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC 
ATATCCGCTG AATATGCCGA CATCTTCTGC CAGTTCAACT TCATCCATCG CCATGACGAG 
GCCTTCTCTA CGGAGCCCCT GAAGAACACG GGGAGGGGAC CCCCGCTCGG CTTCTACCAC 
GTCCAAAACA TTGCGGTGGA GGTGACCAGG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
CTCGTGTTCG AGGTCTTCGG GCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAC 
GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC CACTTCCCAC GGGTCATGCC GCTGTCCAAG 
CCAGTGCCGG CCACCAAGCT CAGCACGCTG ACGCGACCCT GCCCGGGCCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTCGAGATC TGCGAGCTGG AGGCCAACGG CGACTACATC 
CCCGCAGTGG TGGATCACCG CGGCGGGATG CCGTGCATGG GGACCTTCCT GCTCCACCAG 
GGCATCCAGA GACGGATCAC CGTGACCCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC ATCCGAAACA CCCCGGAAAC GGACGAGTCC 
CTGATTGACC CTAATATCTT GTCGCTCAGC ATCCTCTCTT CTGGATACAT CTGCCCAGCC 
CAGGATGACC GAACCTTTTA CCAGTTTGAG GCTGCGTGGG ACAGCTCCAT GCACAACTCC 
CTCCTGCTGA ACCGGGTCAC CCCTTACCGA GAGAAGATTT ACATGACCCT CTCTGCTTAT 
ATCGAGATGG AGAACTGCAC GCAGCCGGCC GTGGTCACCA AAGACTTCTG CATGGTCTTC 
TACTCGCGCG ACGCCAAGCT GCCCGCCTCG CGCTCCATCC GCAACCTGTT CGGCAGTGGG 
AGCCTGCGGG CCTCAGAGAG CAACCGCGTG ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC 
GTGGCTGACG CGGGCAGCCC AGGCATGCAG CGGCGGCGCC GGCGGGTGCT GGACACATCC 
GTGGCCTACG TCCGCGGCGA GGAGAACCTG GCGGGCTGGA GGCCGCGTAG TGACAGCCTC 
ATCCTGGACC ACCAGTGGGA GCTGGAGAAG CTGAGTCTCC TGCAGGAGGT GGAGAAGACC 
AGGCACTACC TGTTGCTGCG AGAGAAGCTG GAGGCGGCCC AGCGGCCCGG CCCCGAGACG 
CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG GCCCACGGCT CCTCCAGTGC CTCCTCCCCG 
CTCACGGCTG AGGCCCGGCC AGCATCCCTG GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG 
GCTGTCAAGT GCTTACGGCT CCTCACCCAC TCCTTCAACA GAGAGTACAC CCACAGCCAC 
GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC TCTGAGATGT CGGTCACCCT GCTGCGGGAC 
CCGTCCATGT CCCCGCTGGG GGCCGCCACG CTCACTCCCT CCTCTACCTG CCCCTCCCTG 
GTCGAGGGCC GGTATGGGGC CACCGACCTG AGGACCCCAC AGCCCTGCTC CCGGCCCGCC 
AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA GATGCCAAGA AGCTCCCCTC CCCTGCCCGG 
GCAGCGGAAG CCGACAAGGA GCCCCAGCGC CTGCTGGTGC CTGACATCCA GGAAATCCGT 
GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC CTGCACTTCC TGGAGCCGCA CACGGCCGGC 
TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG CCCTACGCCT ACATGTACAA CCACGACAAG 
GACGCCGTGG AGCGCTTCGT GCTCAACCTG TCCACCGCCC AGGTGGAGTA CAGCGAGGAC 
CAGCAGGCGA TGCTCAAGAC CCCCAACACG TTCGCGGTGT GCACGGAGCA CCGGGGCATC 
TTGCTGCAGG CCAGCAGTGA CAAGGACATG CACGACTGGC TGTACGCCTT CAACCCCCTG 
CTGGCCGGGA CCATCCGGTC CAAGCTGTCC AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010802233.1
CDS	323..5662
Translation	MAGASVKVAVRVRPFNSREMSRESKCIIQMSGSTTTIVNPKQPKETPKSFSFDYSYWSHTSPEDINYASQKQVYRDIGEEMLQHAFEGYNVCIFAYGQTGAGKSYTMMGKQEKDQQGIIPQLCEDLFSRINDTTNDNMSYSVEVSYMEIYCERVRDLLNPKNKGNLRVREHPLLGPYVEDLSKLAVTSYNDIQDLMDSGNKARTVAATNMNETSSRSHAVFNIIFTQKRHDAETNITTEKVSKVSLVDLAGSERADSTGAKGTRLKEGANINKSLTTLGKVISALAEMDSGPNKNKKKKKTDFIPYRDSVLTWLLRENLGGNSRTAMVAALSPADINYDETLSTLRYADRAKQIRCNAVINEDPNNKLIRELKDEVTRLRDLLYAQGLGDITDMTSALVGMSPSSSLSALSSRAASVSSLHERILFAPGSEEAIERLKETEKIIAELNETWEEKLRRTEAIRMEREALLAEMGVAMREDGGTLGVFSPKKTPHLVNLNEDPLMSECLLYYIKDGITRVGREDAEKRQDIVLSGHFIKEEHCVFRSDSCGGSEAVVTLEPCEGADTYVNGKKVTEPSVLRSGNRIIMGKSHVFRFNHPEQARQERERTPCAETPAEPVDWAFAQRELLEKQGIDMKQEMEQRLQELEDQYRREREEATYLLEQQRLDYESKLEALQKQMDSRYYPEVNEEEEEPEDEVQWTERECELALWAFRKWKWYQFTSLRDLLWGNAIFLKEANAISVELKKKVQFQFVLLTDTLYSPLPPDLLPPEAAKDRETRPFPRTIVAVEVQDQKNGATHYWTLEKLRQRLDLMREMYDRAAEVPSSIIEDCDNVVTGGDPFYDRFPWFRLVGSSVVSGCNSYPLLNTCMSERMAALTPSPTFSSPDSDATEPAEEQSVGEEEEEEEEEEEEEDLQDDVFPEHVLCDGRDPFYDRPPLFSLVGRAFVYLSNLLYPVPLVHRVAVVSEKGEVKGFLRVAVQAISADEEAPDYGSGVRQSGTAKISFDDQHFEKFQAESCPGVGMSRSGTSQEELRIVEGQGQAADSGPSADEVNNNTCSAVTPEGLLDSPEKAALDGPLDAALDHLGLGSTFTFRVTVLQASSISAEYADIFCQFNFIHRHDEAFSTEPLKNTGRGPPLGFYHVQNIAVEVTRSFIEYIKSQPLVFEVFGHYQQHPFPPLCKDVLSPLRPSRRHFPRVMPLSKPVPATKLSTLTRPCPGPCHCKYDLLVYFEICELEANGDYIPAVVDHRGGMPCMGTFLLHQGIQRRITVTLLHETGSHIRWKEVRELVVGRIRNTPETDESLIDPNILSLSILSSGYICPAQDDRTFYQFEAAWDSSMHNSLLLNRVTPYREKIYMTLSAYIEMENCTQPAVVTKDFCMVFYSRDAKLPASRSIRNLFGSGSLRASESNRVTGVYELSLCHVADAGSPGMQRRRRRVLDTSVAYVRGEENLAGWRPRSDSLILDHQWELEKLSLLQEVEKTRHYLLLREKLEAAQRPGPETLSPAASDGSEAHGSSSASSPLTAEARPASLEAPSERQRELAVKCLRLLTHSFNREYTHSHVCVSASESKLSEMSVTLLRDPSMSPLGAATLTPSSTCPSLVEGRYGATDLRTPQPCSRPASPEPEPLPEVDAKKLPSPARAAEADKEPQRLLVPDIQEIRVSPIVSKKGYLHFLEPHTAGWAKRFVVVRRPYAYMYNHDKDAVERFVLNLSTAQVEYSEDQQAMLKTPNTFAVCTEHRGILLQASSDKDMHDWLYAFNPLLAGTIRSKLSRRRSAQMRV

Target ORF information:

RefSeq Version	XM_010803931.2
Organism	Bos taurus(cattle)
Definition	Bos taurus kinesin family member 1A (KIF1A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803931.2

ORF Insert Sequence:

ATGGCCGGGG CCTCGGTGAA GGTGGCGGTG CGCGTCCGCC CCTTCAACTC CCGGGAGATG 
AGCCGCGAGT CCAAGTGCAT CATCCAGATG TCGGGGAGCA CCACCACCAT CGTTAACCCC 
AAACAGCCCA AGGAGACGCC CAAAAGCTTC AGCTTCGACT ACTCCTATTG GTCACACACC 
TCGCCGGAAG ACATCAACTA CGCCTCACAG AAGCAGGTGT ACCGCGACAT TGGTGAGGAG 
ATGCTGCAGC ACGCCTTCGA AGGCTACAAC GTCTGCATCT TCGCCTACGG ACAGACTGGG 
GCTGGCAAGT CCTACACCAT GATGGGCAAG CAGGAGAAGG ACCAGCAGGG CATCATCCCT 
CAGCTCTGCG AGGACCTCTT CTCCCGCATC AACGACACGA CCAACGATAA CATGTCCTAC 
TCCGTGGAGG TCAGCTACAT GGAGATTTAC TGCGAGCGTG TCCGTGACCT CCTGAACCCG 
AAGAACAAGG GCAACCTGCG TGTGCGGGAG CACCCGCTTC TGGGTCCCTA TGTGGAGGAC 
CTGTCCAAGC TGGCGGTCAC CTCCTACAAT GACATCCAAG ACCTCATGGA CTCAGGGAAC 
AAGGCCCGGA CGGTGGCGGC CACGAACATG AACGAGACCA GCAGCCGCTC CCACGCCGTG 
TTCAACATCA TCTTCACGCA GAAGCGCCAT GACGCGGAGA CCAACATCAC CACAGAGAAA 
GTGAGCAAAG TCAGCCTGGT GGACCTGGCT GGGAGTGAGC GGGCCGACTC CACGGGGGCC 
AAGGGCACGC GCCTGAAGGA GGGAGCCAAC ATCAACAAGT CCCTGACCAC CCTGGGCAAG 
GTCATCTCTG CGCTGGCCGA GATGGATTCT GGGCCCAACA AGAACAAAAA GAAGAAGAAG 
ACAGACTTCA TTCCCTACAG AGACTCGGTG CTGACGTGGC TCCTCCGGGA AAACCTAGGC 
GGGAACTCCA GGACCGCGAT GGTGGCGGCG CTGAGCCCCG CAGACATCAA CTATGACGAG 
ACGCTGAGCA CCCTGAGGTA CGCCGACCGG GCCAAGCAGA TCCGCTGCAA CGCTGTCATC 
AACGAGGACC CCAACAACAA GCTGATCCGA GAGCTGAAGG ACGAGGTGAC GCGGCTGCGG 
GACCTGCTCT ACGCCCAGGG TCTGGGCGAC ATCACCGACA TGACCAGCGC CCTGGTGGGC 
ATGAGCCCCT CGTCCTCACT CTCGGCCCTG TCCAGCCGTG CCGCCTCCGT GTCCAGCCTG 
CACGAGCGCA TCTTGTTTGC CCCGGGCAGC GAGGAGGCCA TCGAAAGGCT GAAGGAGACA 
GAAAAGATCA TCGCTGAGCT CAACGAGACC TGGGAGGAGA AGCTGCGGCG GACGGAAGCC 
ATCCGGATGG AGAGGGAAGC ACTGCTGGCC GAGATGGGCG TGGCCATGCG GGAGGACGGT 
GGCACGCTGG GGGTGTTCTC TCCCAAAAAG ACGCCACATC TGGTCAACCT GAATGAGGAC 
CCGCTCATGT CCGAGTGCCT GCTCTACTAC ATCAAGGATG GGATCACCAG GGTGGGCCGG 
GAGGACGCGG AGAAGCGGCA GGACATTGTT CTGAGCGGGC ACTTCATCAA GGAGGAGCAC 
TGTGTCTTCC GGAGCGACTC ATGTGGGGGA AGTGAAGCTG TGGTGACCCT AGAGCCCTGT 
GAAGGGGCGG ACACCTATGT CAATGGCAAG AAAGTCACGG AGCCCAGCGT CCTGCGGTCA 
GGAAACCGCA TCATCATGGG CAAGAGCCAC GTGTTCCGGT TCAACCACCC TGAGCAGGCG 
CGTCAGGAGC GCGAGCGCAC GCCCTGCGCA GAGACGCCGG CCGAGCCCGT GGACTGGGCC 
TTCGCCCAGC GCGAGCTGCT GGAGAAGCAG GGCATTGACA TGAAGCAGGA GATGGAGCAG 
AGGCTCCAGG AGCTGGAGGA CCAGTACCGC CGGGAGCGCG AGGAGGCCAC CTACCTGCTG 
GAGCAGCAGC GGCTGGACTA CGAGAGCAAA CTGGAGGCCC TGCAGAAGCA GATGGACTCC 
AGGTACTATC CCGAGGTGAA CGAGGAGGAG GAGGAGCCGG AGGACGAAGT CCAGTGGACC 
GAGCGAGAGT GCGAGCTGGC ACTGTGGGCC TTCCGGAAGT GGAAGTGGTA CCAGTTCACA 
TCATTGCGGG ACCTGCTGTG GGGCAACGCC ATCTTCCTCA AGGAGGCCAA TGCCATCAGC 
GTGGAGCTCA AGAAGAAGGT CCAGTTCCAG TTTGTCCTCC TCACGGACAC ACTCTACTCC 
CCGCTGCCAC CTGACCTGCT GCCCCCGGAG GCCGCCAAAG ACCGAGAGAC TCGCCCCTTC 
CCGCGCACCA TCGTGGCCGT GGAGGTCCAA GACCAGAAGA ACGGGGCCAC CCACTACTGG 
ACGCTGGAGA AACTCAGGCA GCGGCTAGAC CTGATGCGGG AGATGTATGA CCGGGCGGCC 
GAGGTGCCCT CCAGCATCAT CGAGGACTGT GACAACGTGG TGACCGGAGG AGACCCCTTC 
TACGACCGCT TCCCCTGGTT CCGGCTGGTG GGCAGTTCAG TCGTCTCTGG CTGCAACAGC 
TATCCTCTTC TCAACACATG CATGAGCGAG CGCATGGCTG CTCTCACCCC CTCCCCCACC 
TTCTCGAGCC CCGACTCCGA CGCCACCGAG CCTGCCGAGG AGCAGAGCGT GGGGGAGGAG 
GAGGAGGAGG AGGAGGAGGA GGAGGAGGAG GAGGACCTGC AGGACGACGT CTTTCCGGAG 
CACGTGCTGT GCGACGGCCG GGACCCGTTT TACGACCGGC CCCCCCTGTT CAGTTTAGTA 
GGAAGGGCCT TCGTGTACCT CAGCAACCTG CTGTACCCCG TGCCGCTGGT GCACCGCGTG 
GCCGTCGTCA GCGAGAAGGG CGAGGTGAAG GGCTTCCTGC GCGTGGCCGT GCAGGCCATC 
TCAGCCGACG AGGAGGCCCC TGACTACGGC TCCGGCGTCC GCCAGTCGGG AACAGCCAAA 
ATCTCCTTTG ACGACCAGCA TTTTGAGAAG TTCCAGGCCG AGTCCTGCCC CGGGGTGGGC 
ATGTCCCGCT CAGGGACCTC CCAGGAGGAG CTGCGGATTG TGGAAGGCCA GGGCCAGGCC 
GCAGACTCCG GGCCCTCAGC CGACGAGGTC AACAACAACA CCTGTTCAGC GGTGACCCCG 
GAAGGCCTCC TGGACAGCCC TGAGAAAGCC GCCCTGGATG GGCCCCTGGA CGCCGCCCTG 
GACCACCTCG GCCTGGGCAG CACCTTCACC TTCCGCGTGA CCGTCCTGCA GGCGTCCAGC 
ATATCCGCTG AATATGCCGA CATCTTCTGC CAGTTCAACT TCATCCATCG CCATGACGAG 
GCCTTCTCTA CGGAGCCCCT GAAGAACACG GGGAGGGGAC CCCCGCTCGG CTTCTACCAC 
GTCCAAAACA TTGCGGTGGA GGTGACCAGG TCCTTCATCG AGTACATCAA GAGCCAGCCC 
CTCGTGTTCG AGGTCTTCGG GCACTACCAG CAGCACCCGT TCCCGCCCCT CTGCAAGGAC 
GTGCTCAGCC CCCTGAGGCC CTCTCGCCGC CACTTCCCAC GGGTCATGCC GCTGTCCAAG 
CCAGTGCCGG CCACCAAGCT CAGCACGCTG ACGCGACCCT GCCCGGGCCC CTGCCACTGC 
AAGTACGACC TGCTGGTCTA CTTCGAGATC TGCGAGCTGG AGGCCAACGG CGACTACATC 
CCCGCAGTGG TGGATCACCG CGGCGGGATG CCGTGCATGG GGACCTTCCT GCTCCACCAG 
GGCATCCAGA GACGGATCAC CGTGACCCTG CTGCACGAGA CGGGCAGCCA CATCCGCTGG 
AAGGAAGTGC GGGAGCTGGT GGTGGGTCGC ATCCGAAACA CCCCGGAAAC GGACGAGTCC 
CTGATTGACC CTAATATCTT GTCGCTCAGC ATCCTCTCTT CTGGATACAT CTGCCCAGCC 
CAGGATGACC GAACCTTTTA CCAGTTTGAG GCTGCGTGGG ACAGCTCCAT GCACAACTCC 
CTCCTGCTGA ACCGGGTCAC CCCTTACCGA GAGAAGATTT ACATGACCCT CTCTGCTTAT 
ATCGAGATGG AGAACTGCAC GCAGCCGGCC GTGGTCACCA AAGACTTCTG CATGGTCTTC 
TACTCGCGCG ACGCCAAGCT GCCCGCCTCG CGCTCCATCC GCAACCTGTT CGGCAGTGGG 
AGCCTGCGGG CCTCAGAGAG CAACCGCGTG ACGGGCGTGT ACGAGCTGAG TCTGTGCCAC 
GTGGCTGACG CGGGCAGCCC AGGCATGCAG CGGCGGCGCC GGCGGGTGCT GGACACATCC 
GTGGCCTACG TCCGCGGCGA GGAGAACCTG GCGGGCTGGA GGCCGCGTAG TGACAGCCTC 
ATCCTGGACC ACCAGTGGGA GCTGGAGAAG CTGAGTCTCC TGCAGGAGGT GGAGAAGACC 
AGGCACTACC TGTTGCTGCG AGAGAAGCTG GAGGCGGCCC AGCGGCCCGG CCCCGAGACG 
CTGTCCCCCG CCGCCAGTGA CGGCTCCGAG GCCCACGGCT CCTCCAGTGC CTCCTCCCCG 
CTCACGGCTG AGGCCCGGCC AGCATCCCTG GAGGCGCCCA GCGAGCGGCA GCGGGAGCTG 
GCTGTCAAGT GCTTACGGCT CCTCACCCAC TCCTTCAACA GAGAGTACAC CCACAGCCAC 
GTCTGCGTCA GCGCCAGCGA GAGCAAGCTC TCTGAGATGT CGGTCACCCT GCTGCGGGAC 
CCGTCCATGT CCCCGCTGGG GGCCGCCACG CTCACTCCCT CCTCTACCTG CCCCTCCCTG 
GTCGAGGGCC GGTATGGGGC CACCGACCTG AGGACCCCAC AGCCCTGCTC CCGGCCCGCC 
AGCCCGGAGC CTGAGCCCCT GCCCGAGGTA GATGCCAAGA AGCTCCCCTC CCCTGCCCGG 
GCAGCGGAAG CCGACAAGGA GCCCCAGCGC CTGCTGGTGC CTGACATCCA GGAAATCCGT 
GTCAGCCCCA TCGTCTCCAA GAAGGGCTAC CTGCACTTCC TGGAGCCGCA CACGGCCGGC 
TGGGCCAAGC GCTTCGTGGT GGTGCGGCGG CCCTACGCCT ACATGTACAA CCACGACAAG 
GACGCCGTGG AGCGCTTCGT GCTCAACCTG TCCACCGCCC AGGTGGAGTA CAGCGAGGAC 
CAGCAGGCGA TGCTCAAGAC CCCCAACACG TTCGCGGTGT GCACGGAGCA CCGGGGCATC 
TTGCTGCAGG CCAGCAGTGA CAAGGACATG CACGACTGGC TGTACGCCTT CAACCCCCTG 
CTGGCCGGGA CCATCCGGTC CAAGCTGTCC AGAAGGAGGT CTGCCCAGAT GCGGGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KIF1A
Entrez Gene ID	527883
Full Name	kinesin family member 1A
General protein information	Preferred Names kinesin family member 1A Names kinesin-like protein KIF1A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	KIF1A	XP_002799156.1
Gallus gallus (chicken)	KIF1A	XP_003641781.1
Rattus norvegicus (Norway rat)	Kif1a	XP_006245623.1
Drosophila melanogaster (fruit fly)	unc-104	NP_001246373.1
Anopheles gambiae (African malaria mosquito)	KIF1A_ANOGA	XP_314493.3
Pan troglodytes (chimpanzee)	KIF1A	XP_003309612.1
Bos taurus (cattle)	KIF1A	XP_005205116.1
Mus musculus (house mouse)	Kif1a	NP_032466.2
Caenorhabditis elegans (roundworm)	unc-104	NP_001022041.1
Homo sapiens (human)	KIF1A	NP_004312.2
Canis lupus familiaris (dog)	KIF1A	NP_001161945.1
Danio rerio (zebrafish)	kif1a	XP_005166002.1
Danio rerio (zebrafish)	kif1ab	XP_005171271.1
Xenopus tropicalis (tropical clawed frog)	kif1a	XP_002933380.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KIF1A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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