EFL1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015468484.1	XP_015323970.1	elongation factor Tu GTP-binding domain-containing protein 1
XM_015468485.1	XP_015323971.1	elongation factor Tu GTP-binding domain-containing protein 1
XM_005221896.3	XP_005221953.1	elongation factor Tu GTP-binding domain-containing protein 1
XM_005221897.4	XP_005221954.1	elongation factor-like GTPase 1
XM_002696606.4	XP_002696652.1	elongation factor Tu GTP-binding domain-containing protein 1
XM_005221897.3	XP_005221954.1	elongation factor Tu GTP-binding domain-containing protein 1
XM_015468483.1	XP_015323969.1	elongation factor Tu GTP-binding domain-containing protein 1
XM_002696606.5	XP_002696652.1	elongation factor-like GTPase 1
XM_005221896.4	XP_005221953.1	elongation factor-like GTPase 1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa30906	XM_002696606.5 Latest version!	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30906	XM_005221896.4 Latest version!	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30906	XM_005221897.4 Latest version!	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30906	XM_015468484.1 Latest version!	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30906	XM_015468483.1 Latest version!	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30906	XM_015468485.1 Latest version!	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa30906	XM_005221897.3	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30906	XM_002696606.4	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa30906	XM_005221896.3	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

EFL1 ( XM_015468484.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323970.1 Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30906
Clone ID Related Accession (Same CDS sequence)	XM_015468484.1 , XM_015468485.1 , XM_005221896.3 , XM_005221897.4 , XM_002696606.4 , XM_005221897.3 , XM_015468483.1 , XM_002696606.5 , XM_005221896.4
Accession Version	XM_015468484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	elongation factor Tu GTP-binding domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646532.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323970.1
CDS	582..3971
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAEGDKEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAEREAESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSAIDGWGFGIEHFAKIYSQKTGIRKEVLLKTLWGDYYINTKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDKEKTDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISQAVLAMVCQKLPSPLDITAERVEKLMCAGSQTFDSLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAERRERARRRHAEKLAAAQGQAPVEPTQDAGALEMSPQGEEPKGDEQGVESETPKPVSPEGSEQESFIAFARVFSGVARKGKRIFVLGPKYSPLEFLQQVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDFVLKSATLCTSPSCPPFIPLNFEATPIVRVAIEPKHPSEMPQLVKGMKLLNQADPCVQVFIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSNGLITMMTPNKLATLSVRALPLPEEVTQILEESSDLIRSMEQLTSSLNEGKNNPQMIHQKTQEKIWEFKGKLEHHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFHNSVWTGLDGKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQTASNKQNQEPGDLVKERQGEGDTRSGGDEQQELQDYGSEPFEKRASQRGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_015468484.1
Organism	Bos taurus(cattle)
Definition	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468484.1

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_015468485.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323971.1 Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30906
Clone ID Related Accession (Same CDS sequence)	XM_015468484.1 , XM_015468485.1 , XM_005221896.3 , XM_005221897.4 , XM_002696606.4 , XM_005221897.3 , XM_015468483.1 , XM_002696606.5 , XM_005221896.4
Accession Version	XM_015468485.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	elongation factor Tu GTP-binding domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646532.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323971.1
CDS	72..3461
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAEGDKEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAEREAESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSAIDGWGFGIEHFAKIYSQKTGIRKEVLLKTLWGDYYINTKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDKEKTDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISQAVLAMVCQKLPSPLDITAERVEKLMCAGSQTFDSLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAERRERARRRHAEKLAAAQGQAPVEPTQDAGALEMSPQGEEPKGDEQGVESETPKPVSPEGSEQESFIAFARVFSGVARKGKRIFVLGPKYSPLEFLQQVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDFVLKSATLCTSPSCPPFIPLNFEATPIVRVAIEPKHPSEMPQLVKGMKLLNQADPCVQVFIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSNGLITMMTPNKLATLSVRALPLPEEVTQILEESSDLIRSMEQLTSSLNEGKNNPQMIHQKTQEKIWEFKGKLEHHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFHNSVWTGLDGKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQTASNKQNQEPGDLVKERQGEGDTRSGGDEQQELQDYGSEPFEKRASQRGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_015468485.1
Organism	Bos taurus(cattle)
Definition	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468485.1

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_005221896.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221953.1 Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.

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CloneID	OBa30906
Clone ID Related Accession (Same CDS sequence)	XM_015468484.1 , XM_015468485.1 , XM_005221896.3 , XM_005221897.4 , XM_002696606.4 , XM_005221897.3 , XM_015468483.1 , XM_002696606.5 , XM_005221896.4
Accession Version	XM_005221896.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	elongation factor Tu GTP-binding domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221896.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221953.1
CDS	582..3971
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAEGDKEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAEREAESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSAIDGWGFGIEHFAKIYSQKTGIRKEVLLKTLWGDYYINTKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDKEKTDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISQAVLAMVCQKLPSPLDITAERVEKLMCAGSQTFDSLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAERRERARRRHAEKLAAAQGQAPVEPTQDAGALEMSPQGEEPKGDEQGVESETPKPVSPEGSEQESFIAFARVFSGVARKGKRIFVLGPKYSPLEFLQQVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDFVLKSATLCTSPSCPPFIPLNFEATPIVRVAIEPKHPSEMPQLVKGMKLLNQADPCVQVFIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSNGLITMMTPNKLATLSVRALPLPEEVTQILEESSDLIRSMEQLTSSLNEGKNNPQMIHQKTQEKIWEFKGKLEHHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFHNSVWTGLDGKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQTASNKQNQEPGDLVKERQGEGDTRSGGDEQQELQDYGSEPFEKRASQRGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_005221896.3
Organism	Bos taurus(cattle)
Definition	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221896.3

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_005221897.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221954.1 Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30906
Clone ID Related Accession (Same CDS sequence)	XM_015468484.1 , XM_015468485.1 , XM_005221896.3 , XM_005221897.4 , XM_002696606.4 , XM_005221897.3 , XM_015468483.1 , XM_002696606.5 , XM_005221896.4
Accession Version	XM_005221897.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	elongation factor-like GTPase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221897.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221954.1
CDS	90..3479
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAEGDKEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAEREAESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSAIDGWGFGIEHFAKIYSQKTGIRKEVLLKTLWGDYYINTKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDKEKTDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISQAVLAMVCQKLPSPLDITAERVEKLMCAGSQTFDSLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAERRERARRRHAEKLAAAQGQAPVEPTQDAGALEMSPQGEEPKGDEQGVESETPKPVSPEGSEQESFIAFARVFSGVARKGKRIFVLGPKYSPLEFLQQVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDFVLKSATLCTSPSCPPFIPLNFEATPIVRVAIEPKHPSEMPQLVKGMKLLNQADPCVQVFIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSNGLITMMTPNKLATLSVRALPLPEEVTQILEESSDLIRSMEQLTSSLNEGKNNPQMIHQKTQEKIWEFKGKLEHHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFHNSVWTGLDGKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQTASNKQNQEPGDLVKERQGEGDTRSGGDEQQELQDYGSEPFEKRASQRGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_005221897.4
Organism	Bos taurus(cattle)
Definition	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221897.4

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_002696606.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002696652.1 Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

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CloneID	OBa30906
Clone ID Related Accession (Same CDS sequence)	XM_015468484.1 , XM_015468485.1 , XM_005221896.3 , XM_005221897.4 , XM_002696606.4 , XM_005221897.3 , XM_015468483.1 , XM_002696606.5 , XM_005221896.4
Accession Version	XM_002696606.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	elongation factor Tu GTP-binding domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002696606.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002696652.1
CDS	126..3515
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAEGDKEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAEREAESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSAIDGWGFGIEHFAKIYSQKTGIRKEVLLKTLWGDYYINTKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDKEKTDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISQAVLAMVCQKLPSPLDITAERVEKLMCAGSQTFDSLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAERRERARRRHAEKLAAAQGQAPVEPTQDAGALEMSPQGEEPKGDEQGVESETPKPVSPEGSEQESFIAFARVFSGVARKGKRIFVLGPKYSPLEFLQQVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDFVLKSATLCTSPSCPPFIPLNFEATPIVRVAIEPKHPSEMPQLVKGMKLLNQADPCVQVFIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSNGLITMMTPNKLATLSVRALPLPEEVTQILEESSDLIRSMEQLTSSLNEGKNNPQMIHQKTQEKIWEFKGKLEHHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFHNSVWTGLDGKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQTASNKQNQEPGDLVKERQGEGDTRSGGDEQQELQDYGSEPFEKRASQRGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_002696606.4
Organism	Bos taurus(cattle)
Definition	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002696606.4

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_005221897.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221954.1 Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30906
Clone ID Related Accession (Same CDS sequence)	XM_015468484.1 , XM_015468485.1 , XM_005221896.3 , XM_005221897.4 , XM_002696606.4 , XM_005221897.3 , XM_015468483.1 , XM_002696606.5 , XM_005221896.4
Accession Version	XM_005221897.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	elongation factor Tu GTP-binding domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104534.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221897.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221954.1
CDS	72..3461
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAEGDKEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAEREAESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSAIDGWGFGIEHFAKIYSQKTGIRKEVLLKTLWGDYYINTKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDKEKTDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISQAVLAMVCQKLPSPLDITAERVEKLMCAGSQTFDSLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAERRERARRRHAEKLAAAQGQAPVEPTQDAGALEMSPQGEEPKGDEQGVESETPKPVSPEGSEQESFIAFARVFSGVARKGKRIFVLGPKYSPLEFLQQVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDFVLKSATLCTSPSCPPFIPLNFEATPIVRVAIEPKHPSEMPQLVKGMKLLNQADPCVQVFIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSNGLITMMTPNKLATLSVRALPLPEEVTQILEESSDLIRSMEQLTSSLNEGKNNPQMIHQKTQEKIWEFKGKLEHHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFHNSVWTGLDGKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQTASNKQNQEPGDLVKERQGEGDTRSGGDEQQELQDYGSEPFEKRASQRGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_005221897.3
Organism	Bos taurus(cattle)
Definition	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221897.3

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_015468483.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323969.1 Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30906
Clone ID Related Accession (Same CDS sequence)	XM_015468484.1 , XM_015468485.1 , XM_005221896.3 , XM_005221897.4 , XM_002696606.4 , XM_005221897.3 , XM_015468483.1 , XM_002696606.5 , XM_005221896.4
Accession Version	XM_015468483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	elongation factor Tu GTP-binding domain-containing protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646532.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323969.1
CDS	126..3515
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAEGDKEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAEREAESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSAIDGWGFGIEHFAKIYSQKTGIRKEVLLKTLWGDYYINTKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDKEKTDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISQAVLAMVCQKLPSPLDITAERVEKLMCAGSQTFDSLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAERRERARRRHAEKLAAAQGQAPVEPTQDAGALEMSPQGEEPKGDEQGVESETPKPVSPEGSEQESFIAFARVFSGVARKGKRIFVLGPKYSPLEFLQQVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDFVLKSATLCTSPSCPPFIPLNFEATPIVRVAIEPKHPSEMPQLVKGMKLLNQADPCVQVFIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSNGLITMMTPNKLATLSVRALPLPEEVTQILEESSDLIRSMEQLTSSLNEGKNNPQMIHQKTQEKIWEFKGKLEHHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFHNSVWTGLDGKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQTASNKQNQEPGDLVKERQGEGDTRSGGDEQQELQDYGSEPFEKRASQRGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_015468483.1
Organism	Bos taurus(cattle)
Definition	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468483.1

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_002696606.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002696652.1 Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30906
Clone ID Related Accession (Same CDS sequence)	XM_015468484.1 , XM_015468485.1 , XM_005221896.3 , XM_005221897.4 , XM_002696606.4 , XM_005221897.3 , XM_015468483.1 , XM_002696606.5 , XM_005221896.4
Accession Version	XM_002696606.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	elongation factor-like GTPase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002696606.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002696652.1
CDS	142..3531
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAEGDKEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAEREAESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSAIDGWGFGIEHFAKIYSQKTGIRKEVLLKTLWGDYYINTKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDKEKTDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISQAVLAMVCQKLPSPLDITAERVEKLMCAGSQTFDSLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAERRERARRRHAEKLAAAQGQAPVEPTQDAGALEMSPQGEEPKGDEQGVESETPKPVSPEGSEQESFIAFARVFSGVARKGKRIFVLGPKYSPLEFLQQVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDFVLKSATLCTSPSCPPFIPLNFEATPIVRVAIEPKHPSEMPQLVKGMKLLNQADPCVQVFIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSNGLITMMTPNKLATLSVRALPLPEEVTQILEESSDLIRSMEQLTSSLNEGKNNPQMIHQKTQEKIWEFKGKLEHHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFHNSVWTGLDGKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQTASNKQNQEPGDLVKERQGEGDTRSGGDEQQELQDYGSEPFEKRASQRGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_002696606.5
Organism	Bos taurus(cattle)
Definition	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002696606.5

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EFL1 ( XM_005221896.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221953.1 Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30906
Clone ID Related Accession (Same CDS sequence)	XM_015468484.1 , XM_015468485.1 , XM_005221896.3 , XM_005221897.4 , XM_002696606.4 , XM_005221897.3 , XM_015468483.1 , XM_002696606.5 , XM_005221896.4
Accession Version	XM_005221896.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	elongation factor-like GTPase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221896.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221953.1
CDS	611..4000
Translation	MVLNSLDKMIQLQKNTANIRNICVLAHVDHGKTTLADCLISSNGIISSRLAGKLRYMDSREDEQVRGITMKSSAISLHYAEGDKEYLINLIDSPGHVDFSSEVSTAVRICDGCIIVVDAVEGVCPQTQAVLRQAWLENIRPVLVINKIDRLIVELKFTPQEAYSHLKNILEQINALTGTLFTSKVLEERAEREAESQVNPNSEQGEQVYDWSTGLEDTDDSHLYFSPDQGNVVFTSAIDGWGFGIEHFAKIYSQKTGIRKEVLLKTLWGDYYINTKAKKIMKVDQAKGKKPLFVQLILENIWSLYDAVLKKDKEKTDKIVTSLGLKIGAREARHSDPKVQINAICSQWLPISQAVLAMVCQKLPSPLDITAERVEKLMCAGSQTFDSLPPETQALKAAFMKCGSEDTAPVIIFVSKMFAVDAKALPQNKPRPLTQEEIAERRERARRRHAEKLAAAQGQAPVEPTQDAGALEMSPQGEEPKGDEQGVESETPKPVSPEGSEQESFIAFARVFSGVARKGKRIFVLGPKYSPLEFLQQVPLGFSAPLEDLPPVPHMACCTLENLYLLMGRELEDLEEVPPGNVLGIGGLQDFVLKSATLCTSPSCPPFIPLNFEATPIVRVAIEPKHPSEMPQLVKGMKLLNQADPCVQVFIQETGEHVLVTAGEVHLQRCLDDLKERFAKIEISVSEPIIPFRETITKPPKVDMVNEEIGKQQKVAVIHQTKEDQSKIPEGIQVDSNGLITMMTPNKLATLSVRALPLPEEVTQILEESSDLIRSMEQLTSSLNEGKNNPQMIHQKTQEKIWEFKGKLEHHLTGRKWRNTVDQIWSFGPRKCGPNILVNKSEDFHNSVWTGLDGKASKEASRYRDLGNSIVSGFQLATLSGPMCEEPLMGVCFVLEKWDLSKFEEQTASNKQNQEPGDLVKERQGEGDTRSGGDEQQELQDYGSEPFEKRASQRGESPLTDCYGPFSGQLIATMKEACRYALQVKPQRLMAAMYTCDIMATSDVLGRVYAVLSKREGRVLQEEMKEGTDMFIIKAVLPVAESFGFADEIRKRTSGLASPQLVFSHWEIIPSDPFWVPTTEEEYLHFGEKADSENQARKYMNAVRKRKGLYVEEKIVEHAEKQRTLSKNK

Target ORF information:

RefSeq Version	XM_005221896.4
Organism	Bos taurus(cattle)
Definition	Bos taurus elongation factor like GTPase 1 (EFL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221896.4

ORF Insert Sequence:

ATGGTGCTCA ATAGTTTGGA TAAGATGATT CAACTCCAGA AGAACACTGC TAACATTCGG 
AATATCTGCG TTTTGGCTCA CGTAGACCAT GGAAAAACTA CTCTGGCTGA CTGTCTTATA 
TCTAGCAATG GAATCATCTC CAGCCGCCTG GCAGGCAAGT TGAGGTACAT GGACAGCAGA 
GAAGATGAAC AGGTCCGAGG GATCACTATG AAATCCAGTG CCATCTCACT GCATTATGCG 
GAAGGCGACA AGGAGTACCT GATTAACCTA ATAGACTCTC CAGGTCACGT GGACTTCTCC 
TCAGAAGTGT CCACGGCTGT TCGCATCTGT GACGGGTGCA TCATCGTGGT GGACGCTGTG 
GAAGGGGTCT GCCCGCAGAC ACAGGCAGTT CTGCGGCAAG CCTGGCTCGA AAACATCCGT 
CCAGTTTTAG TGATCAATAA GATAGATCGC TTGATAGTGG AACTGAAATT CACCCCACAG 
GAGGCCTACT CTCACCTCAA GAATATATTA GAACAGATTA ATGCACTCAC AGGAACTCTT 
TTTACTTCTA AAGTGCTAGA AGAAAGAGCG GAGAGAGAGG CTGAATCCCA GGTGAACCCG 
AATTCTGAAC AAGGAGAGCA GGTGTATGAC TGGAGCACCG GCTTGGAGGA CACGGATGAC 
TCGCACCTGT ACTTCTCACC TGACCAGGGG AATGTGGTGT TTACAAGTGC AATAGATGGG 
TGGGGCTTTG GAATTGAGCA CTTTGCCAAA ATCTACAGTC AAAAAACTGG CATCAGAAAG 
GAAGTTCTTT TGAAAACATT GTGGGGAGAT TATTATATAA ATACGAAGGC TAAGAAGATT 
ATGAAGGTGG ATCAGGCAAA AGGAAAGAAA CCTTTATTTG TACAGTTGAT CCTGGAAAAT 
ATATGGAGTT TGTATGATGC TGTCTTGAAA AAGGACAAAG AAAAAACTGA TAAAATCGTG 
ACTTCTTTAG GATTAAAAAT CGGAGCCCGA GAGGCACGGC ATTCAGATCC TAAAGTTCAG 
ATCAATGCTA TCTGCAGTCA GTGGCTACCC ATATCTCAAG CTGTTCTTGC TATGGTGTGT 
CAGAAGCTTC CTAGTCCCCT CGACATTACA GCTGAGAGAG TGGAGAAACT GATGTGTGCA 
GGATCACAGA CTTTTGATTC TCTCCCACCA GAAACCCAGG CTCTGAAAGC AGCTTTTATG 
AAATGCGGAA GTGAAGATAC CGCTCCAGTT ATTATCTTTG TTTCCAAAAT GTTTGCAGTT 
GATGCTAAGG CTTTGCCTCA GAATAAGCCA AGACCTCTCA CTCAGGAGGA GATTGCTGAG 
AGGCGAGAGC GTGCAAGACG AAGGCATGCG GAGAAGTTGG CAGCGGCGCA GGGCCAGGCA 
CCCGTGGAGC CCACCCAAGA TGCAGGCGCC CTTGAAATGA GTCCACAGGG AGAAGAGCCA 
AAAGGTGATG AGCAGGGGGT GGAAAGTGAG ACCCCCAAAC CCGTGTCCCC GGAAGGAAGC 
GAGCAGGAGT CTTTTATCGC CTTCGCTCGA GTGTTCAGTG GAGTGGCGCG AAAAGGAAAG 
AGAATTTTTG TCCTTGGACC CAAATACAGT CCTCTTGAGT TTTTGCAGCA GGTGCCATTA 
GGCTTCTCAG CTCCATTAGA GGACCTTCCC CCAGTCCCCC ACATGGCGTG CTGTACGTTG 
GAAAACCTGT ACCTTCTGAT GGGAAGGGAA CTGGAAGACC TCGAGGAGGT GCCTCCAGGA 
AATGTGCTAG GAATTGGAGG CCTTCAGGAC TTTGTGCTGA AGTCGGCCAC ACTGTGTACC 
TCACCATCCT GCCCGCCATT CATACCTCTC AACTTCGAAG CCACCCCTAT TGTGAGGGTT 
GCTATTGAAC CGAAACACCC AAGTGAAATG CCTCAACTCG TCAAAGGAAT GAAGCTGTTA 
AACCAAGCTG ATCCTTGTGT CCAGGTTTTC ATTCAGGAAA CAGGAGAGCA CGTTTTAGTC 
ACAGCAGGGG AAGTCCACCT TCAGCGCTGC CTGGATGACT TGAAAGAAAG GTTTGCAAAG 
ATTGAAATCA GTGTATCTGA ACCCATCATT CCATTCAGAG AAACAATCAC AAAACCCCCA 
AAAGTTGACA TGGTCAATGA AGAAATCGGC AAACAACAGA AAGTTGCGGT CATCCACCAA 
ACAAAGGAGG ATCAGAGCAA AATCCCTGAA GGAATCCAGG TTGACTCCAA TGGGCTCATC 
ACCATGATGA CTCCCAACAA ACTTGCCACA CTCAGCGTCC GAGCCCTGCC GCTCCCAGAG 
GAGGTGACTC AGATTCTAGA AGAAAGCAGT GACCTGATTC GTTCTATGGA GCAGTTAACG 
TCCTCTCTAA ACGAGGGCAA AAATAATCCT CAGATGATCC ATCAGAAGAC CCAAGAGAAA 
ATCTGGGAAT TCAAAGGAAA GCTCGAGCAC CACCTCACCG GGAGGAAGTG GAGGAACACG 
GTGGACCAAA TCTGGTCATT TGGCCCAAGA AAATGTGGGC CCAACATACT AGTAAATAAA 
AGTGAGGATT TTCATAATTC AGTGTGGACA GGCCTCGATG GCAAAGCTTC AAAAGAAGCC 
AGTCGGTACC GAGACCTGGG CAACAGCATT GTGAGCGGCT TCCAGCTAGC CACCCTCTCC 
GGCCCCATGT GCGAGGAGCC ACTCATGGGT GTGTGTTTTG TCCTGGAAAA ATGGGACCTG 
AGCAAGTTTG AAGAACAGAC AGCAAGTAAT AAACAGAATC AAGAACCGGG TGATCTGGTG 
AAGGAGAGAC AGGGGGAAGG TGACACCCGC TCAGGTGGAG ATGAACAGCA GGAGCTGCAA 
GACTATGGCT CTGAGCCCTT TGAGAAGAGG GCTTCCCAGA GAGGAGAGTC TCCACTCACT 
GACTGCTACG GGCCCTTTTC AGGACAGCTC ATAGCCACCA TGAAGGAAGC CTGTCGCTAC 
GCACTGCAAG TGAAGCCACA GCGACTGATG GCAGCGATGT ACACATGCGA TATCATGGCC 
ACCAGCGATG TTCTCGGTCG GGTCTACGCG GTCCTGTCAA AGAGAGAAGG ACGAGTACTG 
CAGGAGGAGA TGAAAGAGGG GACAGACATG TTCATCATCA AGGCCGTGCT GCCCGTGGCT 
GAGAGCTTCG GCTTTGCCGA TGAGATCCGG AAGAGGACCA GTGGCCTGGC TAGCCCACAG 
CTGGTATTCA GCCACTGGGA GATAATCCCC AGCGACCCCT TCTGGGTACC AACCACCGAG 
GAGGAGTACC TGCACTTTGG GGAAAAGGCT GATTCCGAGA ACCAGGCCCG GAAGTACATG 
AACGCAGTGC GGAAGCGGAA GGGTCTCTAT GTGGAAGAGA AGATTGTGGA GCACGCAGAA 
AAGCAGAGAA CCCTCAGCAA GAATAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EFL1
Entrez Gene ID	527607
Full Name	elongation factor like GTPase 1
Synonyms	EFTUD1
General protein information	Preferred Names elongation factor like GTPase 1 Names elongation factor-like GTPase 1 elongation factor Tu GTP-binding domain-containing protein 1 elongation factor Tu GTP binding domain containing 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Drosophila melanogaster (fruit fly)	CG33158	NP_788515.1
Schizosaccharomyces pombe (fission yeast)	SPCC553.08c	NP_587766.1
Magnaporthe oryzae (rice blast fungus)	MGG_05265	XP_003712823.1
Arabidopsis thaliana (thale cress)	AT3G22980	NP_188938.1
Oryza sativa (rice)	Os03g0650700	NP_001050786.1
Bos taurus (cattle)	EFTUD1	XP_002696652.1
Danio rerio (zebrafish)	eftud1	XP_697184.5
Saccharomyces cerevisiae (baker's yeast)	RIA1	NP_014236.1
Eremothecium gossypii	AGOS_AFR031C	NP_985578.2
Pan troglodytes (chimpanzee)	EFTUD1	XP_510546.2
	EFTUD1	XP_002804960.1
Mus musculus (house mouse)	Eftud1	NP_780526.2
Rattus norvegicus (Norway rat)	Eftud1	NP_001101004.1
Gallus gallus (chicken)	EFTUD1	NP_001034345.2
Homo sapiens (human)	EFTUD1	NP_078856.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003001	XP_311881.3
Kluyveromyces lactis	KLLA0D07898g	XP_453415.1
Oryza sativa (rice)	Os02g0543300	NP_001047074.1
Canis lupus familiaris (dog)	EFTUD1	XP_850111.1
Neurospora crassa	NCU06278	XP_962882.2
Xenopus tropicalis (tropical clawed frog)	eftud1	XP_002940146.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EFL1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

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