THBS2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	THBS2
Entrez Gene ID	338092
Full Name	thrombospondin 2
Synonyms	TMEM200A,TSP-2
General protein information	Preferred Names thrombospondin 2 Names thrombospondin-2 CISP corticotropin-induced secreted protein transmembrane protein 200A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_176872.1	NP_789861.1	thrombospondin-2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	THBS2	NP_003238.2
	THBS2	XP_001096616.2
Mus musculus (house mouse)	Thbs2	NP_035711.2
Gallus gallus (chicken)	THBS2	NP_001001755.1
Danio rerio (zebrafish)	LOC100535241	XP_003199793.2
Danio rerio (zebrafish)	thbs2b	XP_694690.6
Pan troglodytes (chimpanzee)	THBS2	XP_003311640.2
Canis lupus familiaris (dog)	THBS2	NP_001184085.1
Danio rerio (zebrafish)	thbs2a	XP_005157071.1
Bos taurus (cattle)	THBS2	NP_789861.1
Rattus norvegicus (Norway rat)	Thbs2	NP_001162609.1
Xenopus tropicalis (tropical clawed frog)	thbs2	XP_002931559.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression and localization of members of the thrombospondin family during final follicle maturation and corpus luteum formation and function in the bovine ovary.
Berisha B, Schams D, Rodler D, Sinowatz F, Pfaffl MW
The Journal of reproduction and development62(5)501-510(2016 Oct)

Functions and transcriptional regulation of thrombospondins and their interrelationship with fibroblast growth factor-2 in bovine luteal cells.
Farberov S, Meidan R
Biology of reproduction91(3)58(2014 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Thrombospondin and vascular endothelial growth factor are cyclically expressed in an inverse pattern during bovine ovarian follicle development.
Greenaway J, Gentry PA, Feige JJ, LaMarre J, Petrik JJ
Biology of reproduction72(5)1071-8(2005 May)

Bovine thrombospondin-2: complete complementary deoxyribonucleic acid sequence and immunolocalization in the external zones of the adrenal cortex.
Danik M, Chinn AM, Lafeuillade B, Keramidas M, Aguesse-Germon S, Penhoat A, Chen H, Mosher DF, Chambaz EM, Feige JJ
Endocrinology140(6)2771-80(1999 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

temporal expression and localization pattern of the thrombospondins and their specific receptors in the antral follicles and corpora lutea during the different physiological phases of the estrous cycle and induced luteolysis appear to be compatible with their inhibitory role in the control of ovarian angiogenesis
Title: Expression and localization of members of the thrombospondin family during final follicle maturation and corpus luteum formation and function in the bovine ovary.

Data suggest THBS1 expression predominates in luteal endothelial cells; THBS2 expression predominates in luteinized granulosa cells. Luteinization down-regulates expression of THBS1/THBS2, up-regulates expression of FGF2 (fibroblast growth factor 2).
Title: Functions and transcriptional regulation of thrombospondins and their interrelationship with fibroblast growth factor-2 in bovine luteal cells.

TSP-1 and -2 were coordinately expressed in the extravascular compartment of the ovary during early follicle development. VEGF was inversely expressed, with expression increasing as follicles developed.
Title: Thrombospondin and vascular endothelial growth factor are cyclically expressed in an inverse pattern during bovine ovarian follicle development.

The following THBS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THBS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa01332	NM_176872.1 Latest version!	Bos taurus thrombospondin 2 (THBS2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

THBS2 ( NM_176872.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_789861.1 Bos taurus thrombospondin 2 (THBS2), mRNA.

Price & Availability↑

CloneID	OBa01332
Clone ID Related Accession (Same CDS sequence)	NM_176872.1
Accession Version	NM_176872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3513bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Bos taurus(cattle)
Product	thrombospondin-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X96540.1. Sequence Note: This sequence has been modified as follows: removed 37 bp suspected to be vector contamination from the 5' end. ##Evidence-Data-START## Transcript exon combination :: X96540.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCTGTGGC CACTGCTGCT GCTGGCCCTG TGGGCCTGGC CCAGCGCTCA GGCAGGAGAC 
CAGGACGAGG ACACCGCCTT CGACCTCTTC AGCATCAGCA ACATCAACCG CAAGACCATC 
GGGGCCAAGC AGTTCCGGGG CCCAGACCCC AGCGTGCCCG CCTACCGCTT CGTCCGCTTT 
GACTACATCC CGCCGGTGAG CGCGGAGCAC CTGGGCCGGA TCACCGAGGC CATGCGGCGC 
AAGGAGGGCT TCTTCCTGAC GGCCAGCATG AAGCAGGACC GCCGGTCCCG GGGCACCCTG 
CTGGCACTGG AGGGCCCGGG CGCCACGCAC AGGCAGTTCG AGATCGTGTC CAACGGCCCC 
GCGGACACCC TGGACCTCAC CTACTGGGTG GACGGCACCC AGCACGTCAT CTCCCTGGAG 
GACGTGGGCC TGGCTGACTC CCAGTGGAAG AACGTCACTG TGCAGGTGAC CGGAGAGACC 
TACAGCCTGT ACGTGGGCTG CGACCTGATG GACAGCTTCG CGCTGGACGA GCCCTTCTAC 
GAGCACCTGC AGACCGAGAG GAGCCGGATG TACGTGACCA AGGGCGCAGC CCGCGAGAGC 
CACTTCCGGG GTTTGCTGCA GAACGTCTAC TTAGTCTTTG AAAACTCTGT GGAGGATCTT 
CTGAGCAAGA AAGGTTGTCA GCAGAGCCAG GGAGCTGAAA CCAATGCCAT CAGTGAGAAC 
ACAGAGACGC TGCACCTGAG CCCCATGGTC ACCATGGAGC ACGTGGGCCC GAGTGCAGAG 
AAGAGCCCAG AGGTGTGTGA GCACTCCTGT GAGGAGCTGG GCAGCATGAT CCGAGAGCTC 
TCGGGGCTGC ACGTCATCGT GAACCAGCTC CACGAGAACC TGCGCAAAGT GTCAAATGAC 
AACCAGTTTC TGTGGGAGCT CATCGGCGGG CCGCCCAAGA CCAGGAACGT GTCCGCCTGC 
TGGCAGGACG GCCGGTTCTT TGCCGAGAAT GAGACCTGGG TGGTGGACAG CTGTACCAAG 
TGCACGTGCA AGAAATTTAA GACTGTTTGC CACCAAATCA GCTGCCCGCC CGCGACGTGT 
GCGGACCCCT GGTTCGTGGA GGGCGAGTGC TGCCCGTCCT GCGTCCATGA CGGAGAGGAA 
GGCTGGTCCC CGTGGGCGGA GTGGACGGAG TGCTCAGCCA CCTGCGGGTC GGGCACCCAG 
CAGCGGGGCC GCTCCTGTGA TGTCACCAGC AACACCTGCC TGGGGCCGTC CATCCAGACA 
CGGGCCTGCA GCCTGGGCAG GTGCGACCAC CGCATCCGGC AGGACGGCGG CTGGAGCCAC 
TGGTCGCCGT GGTCCTCCTG CTCAGTGACC TGCGGCGTCG GCAACGTCAC CCGTATCCGC 
CTCTGCAACT CGCCAGTGCC GCAGATGGGG GGCCGGAGCT GCAAGGGCAG CGGCCGTGAG 
ACCAAGGCCT GCCAGGGCCC GCCCTGCCCG GTGGATGGCC GGTGGAGCCC CTGGTCCCCG 
TGGTCGGCCT GCACAGTCAC CTGTGCTGGA GGGATCCGTG AGCGGACACG CGTCTGCAAC 
AGCCCCGAGC CCCAGCACGG GGGCAAGGAC TGCGTGGGGG GCGCCAAGGA GCAACAGATG 
TGTAACAGGA AGAGCTGCCC CATAGACGGC TGCCTGTCCA ACCCCTGCTT CCCTGGAGCT 
GAGTGCAGCA GCTTCCCCGA CGGCTCCTGG TCCTGCGGCT CCTGCCCGGG GGGCTTCCTG 
GGCAACGGCA CCCACTGCGA GGACCTGGAC GAGTGCGCCG TGGTCACGGA CGTGTGCTTC 
GCAACCAGCA AGGCACACCG CTGTGTCAAC ACCAACCCCG GCTACCACTG CCTGCCCTGC 
CCGCCGCGCT ACAAGGGGAA CCAGCCCTTT GGTGTCGGCC TGGAGGCGGC CAGGACTGAG 
AAGCAGGTGT GTGAGCCCGA GAACCCCTGC AAGGACAAGA CCCACAGCTG CCACCGGCAC 
GCCGAGTGCA TCTACCTGGG CCACTTCAGC GACCCCATGT ACAAGTGCGA GTGCCAGACG 
GGCTACGCGG GTGACGGGCT CATCTGCGGG GAGGACTCAG ACCTGGATGG CTGGCCCAAC 
AAGAACCTGG TGTGCGCCAC CAACGCCACG TACCACTGCG TCAAGGATAA CTGCCCCCTC 
CTGCCCAACT CAGGGCAAGA AGACTTCGAC AAGGATGGCA TCGGGGACGC CTGCGACGAC 
GACGATGACA ACGACGGCGT CTCGGACGAG AAGGACAACT GCCAGCTTCT CTTCAACCCC 
CGTCAGTTCG ACTACGACAA AGATGAGGTC GGGGACCGGT GCGACAACTG CCCGTATGTT 
CACAACCCGG CCCAGATCGA CACGGACAAC AACGGGGAGG GTGACGCCTG CTCCGTGGAC 
ATCGACGGGG ACGATGTCTT CAACGAGCGA GACAACTGTC CCTACGTCTA CAACACCGAC 
CAGCGGGACA CCGACGGGGA CGGCGTGGGT GACCACTGTG ACAACTGTCC GCTGGTGCAC 
AACCCCGACC AGACCGACGT GGACAACGAC CTCGTGGGAG ACCAGTGTGA CAACAACGAG 
GACATCGACG AAGACGGCCA CCAGAACAAC CAGGACAACT GCCCCCACAT CCCCAACGCC 
AACCAGGCCG ACCATGACCG CGACGGCCAG GGCGACGCCT GCGACTCGGA CGACGACAAT 
GATGGGATCC CCGACGACAG GGACAACTGC CGGCTGGTCG CCAACCCCGA CCAGGAGGAC 
TCAGATGGCG ACCGGCGTGG GGACGCTTGT AAAGACGACT TCGACAACGA CAGTATTCCT 
GATATTGACG ACGTGTGTCC CGAGAACAAC GCCATCAGCG AGACAGACTT CCGGAACTTC 
CAGATGGTCC ACTTGGACCC CAAGGGCACC ACTCAGATTG ATCCCAACTG GGTCATTCGC 
CATCAAGGCA AAGAGCTGGT GCAGACAGCC AACTCCGACC CTGGCATCGC TGTTGGTTTC 
GACGAGTTCG GGTCTGTCGA CTTCAGCGGC ACGTTCTACG TGAACACGGA CCGCGACGAC 
GACTACGCGG GCTTCGTCTT CGGCTACCAG TCCAGCAGCC GCTTCTACGT GGTGATGTGG 
AAGCAGGTGA CGCAGACCTA CTGGGAGGAC CAGCCCACCC GGGCCTACGG CTACTCGGGG 
GTGTCCCTCA AGGTGGTGAA CTCCACCACG GGCACGGGTG AGCACCTGAG GAACGCCCTG 
TGGCACACGG GCAACACAGA GGGACAGGTT CGCACTCTAT GGCACGACCC CAAGAATATC 
GGCTGGAAGG ACTACACTGC CTACCGGTGG CATCTGACCC ACAGGCCTAA GACAGGCTAC 
ATAAGAGTCT TAGTACATGA AGGAAAACAA GTCATGGCGG ACTCAGGACC CATCTATGAC 
CAAACCTACG CTGGTGGGCG GCTGGGTCTG TTTGTCTTCT CTCAAGAGAT GGTCTACTTC 
TCGGACCTCA AATATGAATG CAGAGATGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_789861.1
CDS	464..3976
Translation	MLWPLLLLALWAWPSAQAGDQDEDTAFDLFSISNINRKTIGAKQFRGPDPSVPAYRFVRFDYIPPVSAEHLGRITEAMRRKEGFFLTASMKQDRRSRGTLLALEGPGATHRQFEIVSNGPADTLDLTYWVDGTQHVISLEDVGLADSQWKNVTVQVTGETYSLYVGCDLMDSFALDEPFYEHLQTERSRMYVTKGAARESHFRGLLQNVYLVFENSVEDLLSKKGCQQSQGAETNAISENTETLHLSPMVTMEHVGPSAEKSPEVCEHSCEELGSMIRELSGLHVIVNQLHENLRKVSNDNQFLWELIGGPPKTRNVSACWQDGRFFAENETWVVDSCTKCTCKKFKTVCHQISCPPATCADPWFVEGECCPSCVHDGEEGWSPWAEWTECSATCGSGTQQRGRSCDVTSNTCLGPSIQTRACSLGRCDHRIRQDGGWSHWSPWSSCSVTCGVGNVTRIRLCNSPVPQMGGRSCKGSGRETKACQGPPCPVDGRWSPWSPWSACTVTCAGGIRERTRVCNSPEPQHGGKDCVGGAKEQQMCNRKSCPIDGCLSNPCFPGAECSSFPDGSWSCGSCPGGFLGNGTHCEDLDECAVVTDVCFATSKAHRCVNTNPGYHCLPCPPRYKGNQPFGVGLEAARTEKQVCEPENPCKDKTHSCHRHAECIYLGHFSDPMYKCECQTGYAGDGLICGEDSDLDGWPNKNLVCATNATYHCVKDNCPLLPNSGQEDFDKDGIGDACDDDDDNDGVSDEKDNCQLLFNPRQFDYDKDEVGDRCDNCPYVHNPAQIDTDNNGEGDACSVDIDGDDVFNERDNCPYVYNTDQRDTDGDGVGDHCDNCPLVHNPDQTDVDNDLVGDQCDNNEDIDEDGHQNNQDNCPHIPNANQADHDRDGQGDACDSDDDNDGIPDDRDNCRLVANPDQEDSDGDRRGDACKDDFDNDSIPDIDDVCPENNAISETDFRNFQMVHLDPKGTTQIDPNWVIRHQGKELVQTANSDPGIAVGFDEFGSVDFSGTFYVNTDRDDDYAGFVFGYQSSSRFYVVMWKQVTQTYWEDQPTRAYGYSGVSLKVVNSTTGTGEHLRNALWHTGNTEGQVRTLWHDPKNIGWKDYTAYRWHLTHRPKTGYIRVLVHEGKQVMADSGPIYDQTYAGGRLGLFVFSQEMVYFSDLKYECRDV

Target ORF information:

RefSeq Version	NM_176872.1
Organism	Bos taurus(cattle)
Definition	Bos taurus thrombospondin 2 (THBS2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_176872.1

ORF Insert Sequence:

ATGCTGTGGC CACTGCTGCT GCTGGCCCTG TGGGCCTGGC CCAGCGCTCA GGCAGGAGAC 
CAGGACGAGG ACACCGCCTT CGACCTCTTC AGCATCAGCA ACATCAACCG CAAGACCATC 
GGGGCCAAGC AGTTCCGGGG CCCAGACCCC AGCGTGCCCG CCTACCGCTT CGTCCGCTTT 
GACTACATCC CGCCGGTGAG CGCGGAGCAC CTGGGCCGGA TCACCGAGGC CATGCGGCGC 
AAGGAGGGCT TCTTCCTGAC GGCCAGCATG AAGCAGGACC GCCGGTCCCG GGGCACCCTG 
CTGGCACTGG AGGGCCCGGG CGCCACGCAC AGGCAGTTCG AGATCGTGTC CAACGGCCCC 
GCGGACACCC TGGACCTCAC CTACTGGGTG GACGGCACCC AGCACGTCAT CTCCCTGGAG 
GACGTGGGCC TGGCTGACTC CCAGTGGAAG AACGTCACTG TGCAGGTGAC CGGAGAGACC 
TACAGCCTGT ACGTGGGCTG CGACCTGATG GACAGCTTCG CGCTGGACGA GCCCTTCTAC 
GAGCACCTGC AGACCGAGAG GAGCCGGATG TACGTGACCA AGGGCGCAGC CCGCGAGAGC 
CACTTCCGGG GTTTGCTGCA GAACGTCTAC TTAGTCTTTG AAAACTCTGT GGAGGATCTT 
CTGAGCAAGA AAGGTTGTCA GCAGAGCCAG GGAGCTGAAA CCAATGCCAT CAGTGAGAAC 
ACAGAGACGC TGCACCTGAG CCCCATGGTC ACCATGGAGC ACGTGGGCCC GAGTGCAGAG 
AAGAGCCCAG AGGTGTGTGA GCACTCCTGT GAGGAGCTGG GCAGCATGAT CCGAGAGCTC 
TCGGGGCTGC ACGTCATCGT GAACCAGCTC CACGAGAACC TGCGCAAAGT GTCAAATGAC 
AACCAGTTTC TGTGGGAGCT CATCGGCGGG CCGCCCAAGA CCAGGAACGT GTCCGCCTGC 
TGGCAGGACG GCCGGTTCTT TGCCGAGAAT GAGACCTGGG TGGTGGACAG CTGTACCAAG 
TGCACGTGCA AGAAATTTAA GACTGTTTGC CACCAAATCA GCTGCCCGCC CGCGACGTGT 
GCGGACCCCT GGTTCGTGGA GGGCGAGTGC TGCCCGTCCT GCGTCCATGA CGGAGAGGAA 
GGCTGGTCCC CGTGGGCGGA GTGGACGGAG TGCTCAGCCA CCTGCGGGTC GGGCACCCAG 
CAGCGGGGCC GCTCCTGTGA TGTCACCAGC AACACCTGCC TGGGGCCGTC CATCCAGACA 
CGGGCCTGCA GCCTGGGCAG GTGCGACCAC CGCATCCGGC AGGACGGCGG CTGGAGCCAC 
TGGTCGCCGT GGTCCTCCTG CTCAGTGACC TGCGGCGTCG GCAACGTCAC CCGTATCCGC 
CTCTGCAACT CGCCAGTGCC GCAGATGGGG GGCCGGAGCT GCAAGGGCAG CGGCCGTGAG 
ACCAAGGCCT GCCAGGGCCC GCCCTGCCCG GTGGATGGCC GGTGGAGCCC CTGGTCCCCG 
TGGTCGGCCT GCACAGTCAC CTGTGCTGGA GGGATCCGTG AGCGGACACG CGTCTGCAAC 
AGCCCCGAGC CCCAGCACGG GGGCAAGGAC TGCGTGGGGG GCGCCAAGGA GCAACAGATG 
TGTAACAGGA AGAGCTGCCC CATAGACGGC TGCCTGTCCA ACCCCTGCTT CCCTGGAGCT 
GAGTGCAGCA GCTTCCCCGA CGGCTCCTGG TCCTGCGGCT CCTGCCCGGG GGGCTTCCTG 
GGCAACGGCA CCCACTGCGA GGACCTGGAC GAGTGCGCCG TGGTCACGGA CGTGTGCTTC 
GCAACCAGCA AGGCACACCG CTGTGTCAAC ACCAACCCCG GCTACCACTG CCTGCCCTGC 
CCGCCGCGCT ACAAGGGGAA CCAGCCCTTT GGTGTCGGCC TGGAGGCGGC CAGGACTGAG 
AAGCAGGTGT GTGAGCCCGA GAACCCCTGC AAGGACAAGA CCCACAGCTG CCACCGGCAC 
GCCGAGTGCA TCTACCTGGG CCACTTCAGC GACCCCATGT ACAAGTGCGA GTGCCAGACG 
GGCTACGCGG GTGACGGGCT CATCTGCGGG GAGGACTCAG ACCTGGATGG CTGGCCCAAC 
AAGAACCTGG TGTGCGCCAC CAACGCCACG TACCACTGCG TCAAGGATAA CTGCCCCCTC 
CTGCCCAACT CAGGGCAAGA AGACTTCGAC AAGGATGGCA TCGGGGACGC CTGCGACGAC 
GACGATGACA ACGACGGCGT CTCGGACGAG AAGGACAACT GCCAGCTTCT CTTCAACCCC 
CGTCAGTTCG ACTACGACAA AGATGAGGTC GGGGACCGGT GCGACAACTG CCCGTATGTT 
CACAACCCGG CCCAGATCGA CACGGACAAC AACGGGGAGG GTGACGCCTG CTCCGTGGAC 
ATCGACGGGG ACGATGTCTT CAACGAGCGA GACAACTGTC CCTACGTCTA CAACACCGAC 
CAGCGGGACA CCGACGGGGA CGGCGTGGGT GACCACTGTG ACAACTGTCC GCTGGTGCAC 
AACCCCGACC AGACCGACGT GGACAACGAC CTCGTGGGAG ACCAGTGTGA CAACAACGAG 
GACATCGACG AAGACGGCCA CCAGAACAAC CAGGACAACT GCCCCCACAT CCCCAACGCC 
AACCAGGCCG ACCATGACCG CGACGGCCAG GGCGACGCCT GCGACTCGGA CGACGACAAT 
GATGGGATCC CCGACGACAG GGACAACTGC CGGCTGGTCG CCAACCCCGA CCAGGAGGAC 
TCAGATGGCG ACCGGCGTGG GGACGCTTGT AAAGACGACT TCGACAACGA CAGTATTCCT 
GATATTGACG ACGTGTGTCC CGAGAACAAC GCCATCAGCG AGACAGACTT CCGGAACTTC 
CAGATGGTCC ACTTGGACCC CAAGGGCACC ACTCAGATTG ATCCCAACTG GGTCATTCGC 
CATCAAGGCA AAGAGCTGGT GCAGACAGCC AACTCCGACC CTGGCATCGC TGTTGGTTTC 
GACGAGTTCG GGTCTGTCGA CTTCAGCGGC ACGTTCTACG TGAACACGGA CCGCGACGAC 
GACTACGCGG GCTTCGTCTT CGGCTACCAG TCCAGCAGCC GCTTCTACGT GGTGATGTGG 
AAGCAGGTGA CGCAGACCTA CTGGGAGGAC CAGCCCACCC GGGCCTACGG CTACTCGGGG 
GTGTCCCTCA AGGTGGTGAA CTCCACCACG GGCACGGGTG AGCACCTGAG GAACGCCCTG 
TGGCACACGG GCAACACAGA GGGACAGGTT CGCACTCTAT GGCACGACCC CAAGAATATC 
GGCTGGAAGG ACTACACTGC CTACCGGTGG CATCTGACCC ACAGGCCTAA GACAGGCTAC 
ATAAGAGTCT TAGTACATGA AGGAAAACAA GTCATGGCGG ACTCAGGACC CATCTATGAC 
CAAACCTACG CTGGTGGGCG GCTGGGTCTG TTTGTCTTCT CTCAAGAGAT GGTCTACTTC 
TCGGACCTCA AATATGAATG CAGAGATGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression and localization of members of the thrombospondin family during final follicle maturation and corpus luteum formation and function in the bovine ovary.
The Journal of reproduction and development62(5)501-510(2016 Oct)
Berisha B,Schams D,Rodler D,Sinowatz F,Pfaffl MW

Functions and transcriptional regulation of thrombospondins and their interrelationship with fibroblast growth factor-2 in bovine luteal cells.
Biology of reproduction91(3)58(2014 Sep)
Farberov S,Meidan R

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Thrombospondin and vascular endothelial growth factor are cyclically expressed in an inverse pattern during bovine ovarian follicle development.
Biology of reproduction72(5)1071-8(2005 May)
Greenaway J,Gentry PA,Feige JJ,LaMarre J,Petrik JJ

Bovine thrombospondin-2: complete complementary deoxyribonucleic acid sequence and immunolocalization in the external zones of the adrenal cortex.
Endocrinology140(6)2771-80(1999 Jun)
Danik M,Chinn AM,Lafeuillade B,Keramidas M,Aguesse-Germon S,Penhoat A,Chen H,Mosher DF,Chambaz EM,Feige JJ