NDUFA5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	NDUFA5
Entrez Gene ID	327714
Full Name	NADH:ubiquinone oxidoreductase subunit A5
Synonyms	B13
General protein information	Preferred Names NADH:ubiquinone oxidoreductase subunit A5 Names NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 CI-13kD-B NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5, 13kDa NADH-ubiquinone oxidoreductase 13 kDa-B subunit complex I subunit B13 complex I-13kD-B
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024990499.1	XP_024846267.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform X1
NM_175829.2	NP_787023.1	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5

Pathways from BioSystems

Homologies

	NDUFA5	XP_002803497.1
Pan troglodytes (chimpanzee)	NDUFA5	NP_001065265.1
Rattus norvegicus (Norway rat)	Ndufa5	NP_037117.1
Danio rerio (zebrafish)	ndufa5	NP_001013474.1
Caenorhabditis elegans (roundworm)	CELE_C33A12.1	NP_501688.1
Xenopus tropicalis (tropical clawed frog)	ndufa5	NP_001039265.1
Canis lupus familiaris (dog)	NDUFA5	XP_532446.1
Homo sapiens (human)	NDUFA5	NP_004991.1
Gallus gallus (chicken)	NDUFA5	NP_001091106.1
Drosophila melanogaster (fruit fly)	CG6463	NP_648409.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005621	XP_315631.3
Bos taurus (cattle)	NDUFA5	NP_787023.1
Mus musculus (house mouse)	Ndufa5	NP_080890.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Lemma-Gray P, Valusov? E, Carroll CA, Weintraub ST, Musatov A, Robinson NC
Analytical biochemistry382(2)116-21(2008 Nov)

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Sazanov LA, Peak-Chew SY, Fearnley IM, Walker JE
Biochemistry39(24)7229-35(2000 Jun)

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Walker JE, Arizmendi JM, Dupuis A, Fearnley IM, Finel M, Medd SM, Pilkington SJ, Runswick MJ, Skehel JM
Journal of molecular biology226(4)1051-72(1992 Aug)

The amino acid sequences of two 13 kDa polypeptides and partial amino acid sequence of 30 kDa polypeptide of complex I from bovine heart mitochondria: possible location of iron-sulfur clusters.
Masui R, Wakabayashi S, Matsubara H, Hatefi Y
Journal of biochemistry109(4)534-43(1991 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following NDUFA5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NDUFA5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41844	NM_175829.2 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit A5 (NDUFA5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa233563	XM_024990499.1 Latest version!	Bos taurus NADH:ubiquinone oxidoreductase subunit A5 (NDUFA5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NDUFA5 ( NM_175829.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_787023.1 Bos taurus NADH:ubiquinone oxidoreductase subunit A5 (NDUFA5), mRNA.

Price & Availability↑

CloneID	OBa41844
Clone ID Related Accession (Same CDS sequence)	NM_175829.2
Accession Version	NM_175829.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X63218.1. On Jun 3, 2003 this sequence version replaced NM_175829.1. ##Evidence-Data-START## Transcript exon combination :: X63218.1, EV621354.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145486 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCGGGCT TGCTGAAGAA GACCACTGGC CTGGTGGGAT TGGCTGTTTG TGAAACTCCA 
CATGAGAGGC TAAAAATATT GTATACAAAA ATTCTTGATG TTCTTGGACA CATCCCTAAA 
AATGCAGCAT ATAGAAAGTA TACAGAACAG ATTACAAATG AGAAGCTGAG TATGGTTAAA 
GCGGAACCTG ATGTTAAAAA ACTAGAAGAG CGACTGCAGG GTGGCCAAAT AGAAGAGGTT 
ATTCTTCAGG CTGAAAACGA ACTAAGTCTG GCAAGAAAAA TGATCCAGTG GAAACCATGG 
GAGCCTTTAG TGGAAGAGCC TCCAGCCAGC CAATGGAAAT GGCCAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_787023.1
CDS	4..354
Translation	MAGLLKKTTGLVGLAVCETPHERLKILYTKILDVLGHIPKNAAYRKYTEQITNEKLSMVKAEPDVKKLEERLQGGQIEEVILQAENELSLARKMIQWKPWEPLVEEPPASQWKWPI

Target ORF information:

RefSeq Version	NM_175829.2
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit A5 (NDUFA5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175829.2

ORF Insert Sequence:

ATGGCGGGCT TGCTGAAGAA GACCACTGGC CTGGTGGGAT TGGCTGTTTG TGAAACTCCA 
CATGAGAGGC TAAAAATATT GTATACAAAA ATTCTTGATG TTCTTGGACA CATCCCTAAA 
AATGCAGCAT ATAGAAAGTA TACAGAACAG ATTACAAATG AGAAGCTGAG TATGGTTAAA 
GCGGAACCTG ATGTTAAAAA ACTAGAAGAG CGACTGCAGG GTGGCCAAAT AGAAGAGGTT 
ATTCTTCAGG CTGAAAACGA ACTAAGTCTG GCAAGAAAAA TGATCCAGTG GAAACCATGG 
GAGCCTTTAG TGGAAGAGCC TCCAGCCAGC CAATGGAAAT GGCCAATATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NDUFA5 ( XM_024990499.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846267.1 Bos taurus NADH:ubiquinone oxidoreductase subunit A5 (NDUFA5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa233563
Clone ID Related Accession (Same CDS sequence)	XM_024990499.1
Accession Version	XM_024990499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGCT TGCTGAAGAA GACCACTGGC CTGGTGGGAT TGGCTGTTTG TGAAACTCCA 
CATGAGGAAC CTGATGTTAA AAAACTAGAA GAGCGACTGC AGGGTGGCCA AATAGAAGAG 
GTTATTCTTC AGGCTGAAAA CGAACTAAGT CTGGCAAGAA AAATGATCCA GTGGAAACCA 
TGGGAGCCTT TAGTGGAAGA GCCTCCAGCC AGCCAATGGA AATGGCCAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846267.1
CDS	107..340
Translation	MAGLLKKTTGLVGLAVCETPHEEPDVKKLEERLQGGQIEEVILQAENELSLARKMIQWKPWEPLVEEPPASQWKWPI

Target ORF information:

RefSeq Version	XM_024990499.1
Organism	Bos taurus(cattle)
Definition	Bos taurus NADH:ubiquinone oxidoreductase subunit A5 (NDUFA5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990499.1

ORF Insert Sequence:

ATGGCGGGCT TGCTGAAGAA GACCACTGGC CTGGTGGGAT TGGCTGTTTG TGAAACTCCA 
CATGAGGAAC CTGATGTTAA AAAACTAGAA GAGCGACTGC AGGGTGGCCA AATAGAAGAG 
GTTATTCTTC AGGCTGAAAA CGAACTAAGT CTGGCAAGAA AAATGATCCA GTGGAAACCA 
TGGGAGCCTT TAGTGGAAGA GCCTCCAGCC AGCCAATGGA AATGGCCAAT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Subunit analysis of bovine heart complex I by reversed-phase high-performance liquid chromatography, electrospray ionization-tandem mass spectrometry, and matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
Analytical biochemistry382(2)116-21(2008 Nov)
Lemma-Gray P,Valusov? E,Carroll CA,Weintraub ST,Musatov A,Robinson NC

Resolution of the membrane domain of bovine complex I into subcomplexes: implications for the structural organization of the enzyme.
Biochemistry39(24)7229-35(2000 Jun)
Sazanov LA,Peak-Chew SY,Fearnley IM,Walker JE

Sequences of 20 subunits of NADH:ubiquinone oxidoreductase from bovine heart mitochondria. Application of a novel strategy for sequencing proteins using the polymerase chain reaction.
Journal of molecular biology226(4)1051-72(1992 Aug)
Walker JE,Arizmendi JM,Dupuis A,Fearnley IM,Finel M,Medd SM,Pilkington SJ,Runswick MJ,Skehel JM

The amino acid sequences of two 13 kDa polypeptides and partial amino acid sequence of 30 kDa polypeptide of complex I from bovine heart mitochondria: possible location of iron-sulfur clusters.
Journal of biochemistry109(4)534-43(1991 Apr)
Masui R,Wakabayashi S,Matsubara H,Hatefi Y