PPP3CA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP3CA
Entrez Gene ID	286852
Full Name	protein phosphatase 3 catalytic subunit alpha
General protein information	Preferred Names protein phosphatase 3 catalytic subunit alpha Names serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform CAM-PRP catalytic subunit calcineurin A alpha calmodulin-dependent calcineurin A subunit alpha isoform protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform (calcineurin A alpha)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005207693.4	XP_005207750.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform X1
NM_174787.2	NP_777212.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
XM_005207693.3	XP_005207750.1	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP3CA	NP_001184025.1
Rattus norvegicus (Norway rat)	Ppp3ca	NP_058737.1
Danio rerio (zebrafish)	ppp3cb	NP_001017856.1
Saccharomyces cerevisiae (baker's yeast)	CNA1	NP_013537.1
Neurospora crassa	NCU03804	XP_961193.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001279	XP_321872.5
Kluyveromyces lactis	KLLA0B05489g	XP_451780.1
Eremothecium gossypii	AGOS_AER118C	NP_984978.1
Magnaporthe oryzae (rice blast fungus)	MGG_07456	XP_003711354.1
Bos taurus (cattle)	PPP3CA	NP_777212.1
Danio rerio (zebrafish)	ppp3ca	NP_001185479.1
Schizosaccharomyces pombe (fission yeast)	ppb1	NP_596178.1
Xenopus tropicalis (tropical clawed frog)	ppp3ca	NP_001119980.1
Pan troglodytes (chimpanzee)	PPP3CA	XP_001168163.1
Mus musculus (house mouse)	Ppp3ca	NP_032939.1
Drosophila melanogaster (fruit fly)	CanA-14F	NP_001259622.1
Homo sapiens (human)	PPP3CA	NP_000935.1
	PPP3CA	XP_001108659.1
Gallus gallus (chicken)	PPP3CA	XP_420664.4
Drosophila melanogaster (fruit fly)	Pp2B-14D	NP_523373.2
Caenorhabditis elegans (roundworm)	tax-6	NP_001076658.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Molecular modeling of the calmodulin binding region of calcineurin.
Hoekman JD, Tokheim AM, Spannaus-Martin DJ, Martin BL
The protein journal25(3)175-82(2006 Apr)

Structure of calmodulin bound to a calcineurin peptide: a new way of making an old binding mode.
Ye Q, Li X, Wong A, Wei Q, Jia Z
Biochemistry45(3)738-45(2006 Jan)

Molecular cloning, expression, purification and characterization of calcineurin from bovine cardiac muscle.
Selvakumar P, Lakshmikuttyamma A, Anderson DH, Sharma RK
Biochimie87(11)975-83(2005 Nov)

X-ray structure of calcineurin inhibited by the immunophilin-immunosuppressant FKBP12-FK506 complex.
Griffith JP, Kim JL, Kim EE, Sintchak MD, Thomson JA, Fitzgibbon MJ, Fleming MA, Caron PR, Hsiao K, Navia MA
Cell82(3)507-22(1995 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The calmodulin binding region from residues 389 to 420 was modeled based on the structure of two other proteins having calmodulin binding domains with the same 1-8-14 structural motif as calcineurin.
Title: Molecular modeling of the calmodulin binding region of calcineurin.

The following PPP3CA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP3CA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa43396	NM_174787.2 Latest version!	Bos taurus protein phosphatase 3 catalytic subunit alpha (PPP3CA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa20226	XM_005207693.4 Latest version!	Bos taurus protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
OBa20226	XM_005207693.3	Bos taurus protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP3CA ( NM_174787.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_777212.1 Bos taurus protein phosphatase 3 catalytic subunit alpha (PPP3CA), mRNA.

Price & Availability↑

CloneID	OBa43396
Clone ID Related Accession (Same CDS sequence)	NM_174787.2
Accession Version	NM_174787.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U33868.1. On Jun 3, 2003 this sequence version replaced NM_174787.1. ##Evidence-Data-START## Transcript exon combination :: U33868.1, BC123668.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145418, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTCCCATTTC CTCCAAGTCA CCGGCTTACG GCAAAAGAAG TGTTTGATAA TGATGGGAAA 
CCTCGTGTGG ATATCTTAAA GGCACATCTC ATGAAGGAGG GCAGGCTCGA AGAGACTGTC 
GCATTGAGAA TAATAACAGA GGGGGCTTCA ATTCTTCGAC AGGAAAAAAA CTTGCTGGAT 
ATTGATGCTC CAGTCACAGT TTGTGGGGAC ATCCATGGAC AATTCTTTGA CTTGATGAAA 
CTCTTTGAAG TGGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT CGAATGTGTG CTGTATTTGT GGGCTTTGAA AATTCTTTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGCATGGAT 
GCCTTTGACT GCCTGCCCCT GGCTGCCCTG ATGAACCAGC AGTTCCTGTG TGTACACGGC 
GGTTTGTCTC CAGAGATAAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCTT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCGCTGGA AGATTTTGGA 
AACGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTGCAG CACAATAACT TGTTATCTAT CCTCCGAGCT 
CATGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCC 
TCTCTCATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTCATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTC ACCTGGTCCC TGCCGTTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATATGCTCAG ACGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGAGC AACGGCTGCA GCCAGGAAGG AGGTGATAAG AAATAAGATC 
CGAGCCATTG GCAAGATGGC CAGAGTGTTC TCGGTTCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGGCTGAC GCCCACAGGC ATGCTCCCCA GCGGCGTGCT TTCTGGAGGG 
AAACAAACCC TGCAAAGCGC TACTGTTGAG GCTATTGAGG CTGATGAAGC TATCAAAGGA 
TTTTCACCAC AACATAAGAT CACTAGCTTC GAGGAGGCCA AGGGCTTAGA CCGAATTAAT 
GAGAGGATGC CACCTCGCAG AGATGCCATG CCCTCTGATG CCAACCTTAA CTCCATCAAC 
AAGGCTCTCG CCTCAGAGAC TAACGGCACG GACAGCAATG GCAGTAATAG CAGCAATATC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777212.1
CDS	191..1756
Translation	MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEETVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSATVEAIEADEAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALASETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	NM_174787.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 3 catalytic subunit alpha (PPP3CA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174787.2

ORF Insert Sequence:

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTCCCATTTC CTCCAAGTCA CCGGCTTACG GCAAAAGAAG TGTTTGATAA TGATGGGAAA 
CCTCGTGTGG ATATCTTAAA GGCACATCTC ATGAAGGAGG GCAGGCTCGA AGAGACTGTC 
GCATTGAGAA TAATAACAGA GGGGGCTTCA ATTCTTCGAC AGGAAAAAAA CTTGCTGGAT 
ATTGATGCTC CAGTCACAGT TTGTGGGGAC ATCCATGGAC AATTCTTTGA CTTGATGAAA 
CTCTTTGAAG TGGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT CGAATGTGTG CTGTATTTGT GGGCTTTGAA AATTCTTTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGCATGGAT 
GCCTTTGACT GCCTGCCCCT GGCTGCCCTG ATGAACCAGC AGTTCCTGTG TGTACACGGC 
GGTTTGTCTC CAGAGATAAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCTT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCGCTGGA AGATTTTGGA 
AACGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTGCAG CACAATAACT TGTTATCTAT CCTCCGAGCT 
CATGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCC 
TCTCTCATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTCATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTC ACCTGGTCCC TGCCGTTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATATGCTCAG ACGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGAGC AACGGCTGCA GCCAGGAAGG AGGTGATAAG AAATAAGATC 
CGAGCCATTG GCAAGATGGC CAGAGTGTTC TCGGTTCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGGCTGAC GCCCACAGGC ATGCTCCCCA GCGGCGTGCT TTCTGGAGGG 
AAACAAACCC TGCAAAGCGC TACTGTTGAG GCTATTGAGG CTGATGAAGC TATCAAAGGA 
TTTTCACCAC AACATAAGAT CACTAGCTTC GAGGAGGCCA AGGGCTTAGA CCGAATTAAT 
GAGAGGATGC CACCTCGCAG AGATGCCATG CCCTCTGATG CCAACCTTAA CTCCATCAAC 
AAGGCTCTCG CCTCAGAGAC TAACGGCACG GACAGCAATG GCAGTAATAG CAGCAATATC 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3CA ( XM_005207693.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207750.1 Bos taurus protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20226
Clone ID Related Accession (Same CDS sequence)	XM_005207693.4 , XM_005207693.3
Accession Version	XM_005207693.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207693.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTCCCATTTC CTCCAAGTCA CCGGCTTACG GCAAAAGAAG TGTTTGATAA TGATGGGAAA 
CCTCGTGTGG ATATCTTAAA GGCACATCTC ATGAAGGAGG GCAGGCTCGA AGAGACTGTC 
GCATTGAGAA TAATAACAGA GGGGGCTTCA ATTCTTCGAC AGGAAAAAAA CTTGCTGGAT 
ATTGATGCTC CAGTCACAGT TTGTGGGGAC ATCCATGGAC AATTCTTTGA CTTGATGAAA 
CTCTTTGAAG TGGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT CGAATGTGTG CTGTATTTGT GGGCTTTGAA AATTCTTTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGCATGGAT 
GCCTTTGACT GCCTGCCCCT GGCTGCCCTG ATGAACCAGC AGTTCCTGTG TGTACACGGC 
GGTTTGTCTC CAGAGATAAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCTT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCGCTGGA AGATTTTGGA 
AACGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTGCAG CACAATAACT TGTTATCTAT CCTCCGAGCT 
CATGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCC 
TCTCTCATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTCATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTC ACCTGGTCCC TGCCGTTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATATGCTCAG ACGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGAGC AACGGCTGCA GCCAGGAAGG AGGTGATAAG AAATAAGATC 
CGAGCCATTG GCAAGATGGC CAGAGTGTTC TCGGTTCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCCTGAC GCCCACAGGC ATGCTCCCCA GCGGCGTGCT TTCTGGAGGG 
AAACAAACCC TGCAAAGCGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAGGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CACCTCGCAG AGATGCCATG 
CCCTCTGATG CCAACCTTAA CTCCATCAAC AAGGCTCTCG CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207750.1
CDS	781..2316
Translation	MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEETVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALASETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	XM_005207693.4
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 3 catalytic subunit alpha (PPP3CA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207693.4

ORF Insert Sequence:

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTCCCATTTC CTCCAAGTCA CCGGCTTACG GCAAAAGAAG TGTTTGATAA TGATGGGAAA 
CCTCGTGTGG ATATCTTAAA GGCACATCTC ATGAAGGAGG GCAGGCTCGA AGAGACTGTC 
GCATTGAGAA TAATAACAGA GGGGGCTTCA ATTCTTCGAC AGGAAAAAAA CTTGCTGGAT 
ATTGATGCTC CAGTCACAGT TTGTGGGGAC ATCCATGGAC AATTCTTTGA CTTGATGAAA 
CTCTTTGAAG TGGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT CGAATGTGTG CTGTATTTGT GGGCTTTGAA AATTCTTTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGCATGGAT 
GCCTTTGACT GCCTGCCCCT GGCTGCCCTG ATGAACCAGC AGTTCCTGTG TGTACACGGC 
GGTTTGTCTC CAGAGATAAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCTT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCGCTGGA AGATTTTGGA 
AACGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTGCAG CACAATAACT TGTTATCTAT CCTCCGAGCT 
CATGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCC 
TCTCTCATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTCATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTC ACCTGGTCCC TGCCGTTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATATGCTCAG ACGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGAGC AACGGCTGCA GCCAGGAAGG AGGTGATAAG AAATAAGATC 
CGAGCCATTG GCAAGATGGC CAGAGTGTTC TCGGTTCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCCTGAC GCCCACAGGC ATGCTCCCCA GCGGCGTGCT TTCTGGAGGG 
AAACAAACCC TGCAAAGCGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAGGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CACCTCGCAG AGATGCCATG 
CCCTCTGATG CCAACCTTAA CTCCATCAAC AAGGCTCTCG CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP3CA ( XM_005207693.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207750.1 Bos taurus protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa20226
Clone ID Related Accession (Same CDS sequence)	XM_005207693.4 , XM_005207693.3
Accession Version	XM_005207693.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103964.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207693.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTCCCATTTC CTCCAAGTCA CCGGCTTACG GCAAAAGAAG TGTTTGATAA TGATGGGAAA 
CCTCGTGTGG ATATCTTAAA GGCACATCTC ATGAAGGAGG GCAGGCTCGA AGAGACTGTC 
GCATTGAGAA TAATAACAGA GGGGGCTTCA ATTCTTCGAC AGGAAAAAAA CTTGCTGGAT 
ATTGATGCTC CAGTCACAGT TTGTGGGGAC ATCCATGGAC AATTCTTTGA CTTGATGAAA 
CTCTTTGAAG TGGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT CGAATGTGTG CTGTATTTGT GGGCTTTGAA AATTCTTTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGCATGGAT 
GCCTTTGACT GCCTGCCCCT GGCTGCCCTG ATGAACCAGC AGTTCCTGTG TGTACACGGC 
GGTTTGTCTC CAGAGATAAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCTT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCGCTGGA AGATTTTGGA 
AACGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTGCAG CACAATAACT TGTTATCTAT CCTCCGAGCT 
CATGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCC 
TCTCTCATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTCATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTC ACCTGGTCCC TGCCGTTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATATGCTCAG ACGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGAGC AACGGCTGCA GCCAGGAAGG AGGTGATAAG AAATAAGATC 
CGAGCCATTG GCAAGATGGC CAGAGTGTTC TCGGTTCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCCTGAC GCCCACAGGC ATGCTCCCCA GCGGCGTGCT TTCTGGAGGG 
AAACAAACCC TGCAAAGCGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAGGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CACCTCGCAG AGATGCCATG 
CCCTCTGATG CCAACCTTAA CTCCATCAAC AAGGCTCTCG CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207750.1
CDS	733..2268
Translation	MSEPKAIDPKLSTTDRVVKAVPFPPSHRLTAKEVFDNDGKPRVDILKAHLMKEGRLEETVALRIITEGASILRQEKNLLDIDAPVTVCGDIHGQFFDLMKLFEVGGSPANTRYLFLGDYVDRGYFSIECVLYLWALKILYPKTLFLLRGNHECRHLTEYFTFKQECKIKYSERVYDACMDAFDCLPLAALMNQQFLCVHGGLSPEINTLDDIRKLDRFKEPPAYGPMCDILWSDPLEDFGNEKTQEHFTHNTVRGCSYFYSYPAVCEFLQHNNLLSILRAHEAQDAGYRMYRKSQTTGFPSLITIFSAPNYLDVYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVTEMLVNVLNICSDDELGSEEDGFDGATAAARKEVIRNKIRAIGKMARVFSVLREESESVLTLKGLTPTGMLPSGVLSGGKQTLQSAIKGFSPQHKITSFEEAKGLDRINERMPPRRDAMPSDANLNSINKALASETNGTDSNGSNSSNIQ

Target ORF information:

RefSeq Version	XM_005207693.3
Organism	Bos taurus(cattle)
Definition	Bos taurus protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207693.3

ORF Insert Sequence:

ATGTCCGAGC CCAAGGCAAT TGATCCCAAG TTGTCGACGA CCGACAGGGT GGTGAAAGCT 
GTCCCATTTC CTCCAAGTCA CCGGCTTACG GCAAAAGAAG TGTTTGATAA TGATGGGAAA 
CCTCGTGTGG ATATCTTAAA GGCACATCTC ATGAAGGAGG GCAGGCTCGA AGAGACTGTC 
GCATTGAGAA TAATAACAGA GGGGGCTTCA ATTCTTCGAC AGGAAAAAAA CTTGCTGGAT 
ATTGATGCTC CAGTCACAGT TTGTGGGGAC ATCCATGGAC AATTCTTTGA CTTGATGAAA 
CTCTTTGAAG TGGGGGGATC TCCTGCCAAC ACTCGCTACC TCTTCTTAGG GGACTATGTT 
GACAGAGGGT ACTTCAGTAT CGAATGTGTG CTGTATTTGT GGGCTTTGAA AATTCTTTAC 
CCCAAAACAC TGTTTTTACT TCGTGGAAAT CATGAATGTA GACATCTAAC AGAGTATTTC 
ACATTTAAAC AAGAATGTAA AATAAAGTAT TCAGAACGCG TATATGATGC CTGCATGGAT 
GCCTTTGACT GCCTGCCCCT GGCTGCCCTG ATGAACCAGC AGTTCCTGTG TGTACACGGC 
GGTTTGTCTC CAGAGATAAA CACTTTAGAT GATATCAGAA AATTAGACCG ATTCAAAGAA 
CCACCTGCTT ATGGACCTAT GTGTGATATC CTGTGGTCAG ACCCGCTGGA AGATTTTGGA 
AACGAGAAGA CTCAGGAACA TTTCACTCAC AACACAGTCA GGGGGTGTTC ATACTTCTAC 
AGTTACCCGG CTGTATGTGA ATTCTTGCAG CACAATAACT TGTTATCTAT CCTCCGAGCT 
CATGAAGCCC AAGATGCAGG GTACCGCATG TACAGGAAAA GCCAAACAAC AGGCTTCCCC 
TCTCTCATTA CAATTTTTTC AGCACCAAAT TACTTAGATG TATACAATAA CAAAGCTGCA 
GTATTGAAGT ATGAGAACAA TGTCATGAAT ATCAGGCAAT TCAACTGTTC TCCTCATCCA 
TACTGGCTTC CAAATTTCAT GGATGTTTTC ACCTGGTCCC TGCCGTTTGT TGGGGAAAAA 
GTGACTGAGA TGCTGGTAAA TGTCCTCAAC ATATGCTCAG ACGATGAACT AGGGTCAGAA 
GAAGATGGAT TTGATGGAGC AACGGCTGCA GCCAGGAAGG AGGTGATAAG AAATAAGATC 
CGAGCCATTG GCAAGATGGC CAGAGTGTTC TCGGTTCTCA GAGAAGAGAG TGAGAGTGTG 
CTGACGCTGA AAGGCCTGAC GCCCACAGGC ATGCTCCCCA GCGGCGTGCT TTCTGGAGGG 
AAACAAACCC TGCAAAGCGC TATCAAAGGA TTTTCACCAC AACATAAGAT CACTAGCTTC 
GAGGAGGCCA AGGGCTTAGA CCGAATTAAT GAGAGGATGC CACCTCGCAG AGATGCCATG 
CCCTCTGATG CCAACCTTAA CTCCATCAAC AAGGCTCTCG CCTCAGAGAC TAACGGCACG 
GACAGCAATG GCAGTAATAG CAGCAATATC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Molecular modeling of the calmodulin binding region of calcineurin.
The protein journal25(3)175-82(2006 Apr)
Hoekman JD,Tokheim AM,Spannaus-Martin DJ,Martin BL

Structure of calmodulin bound to a calcineurin peptide: a new way of making an old binding mode.
Biochemistry45(3)738-45(2006 Jan)
Ye Q,Li X,Wong A,Wei Q,Jia Z

Molecular cloning, expression, purification and characterization of calcineurin from bovine cardiac muscle.
Biochimie87(11)975-83(2005 Nov)
Selvakumar P,Lakshmikuttyamma A,Anderson DH,Sharma RK

X-ray structure of calcineurin inhibited by the immunophilin-immunosuppressant FKBP12-FK506 complex.
Cell82(3)507-22(1995 Aug)
Griffith JP,Kim JL,Kim EE,Sintchak MD,Thomson JA,Fitzgibbon MJ,Fleming MA,Caron PR,Hsiao K,Navia MA