PRKG1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKG1
Entrez Gene ID	282004
Full Name	protein kinase, cGMP-dependent, type I
Synonyms	CGKI,cGK 1,cGKI-beta
General protein information	Preferred Names protein kinase, cGMP-dependent, type I Names cGMP-dependent protein kinase 1 CGK 1 alpha cGK 1 beta cGK1 cGKI-alpha cGMP-dependent protein kinase 1, alpha isozyme cGMP-dependent protein kinase 1, beta isozyme cGMP-dependent protein kinase I
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005225353.2	XP_005225410.1	cGMP-dependent protein kinase 1 isoform X1
XM_024985478.1	XP_024841246.1	cGMP-dependent protein kinase 1 isoform X2
XM_005225353.3	XP_005225410.2	cGMP-dependent protein kinase 1 isoform X1
NM_174436.2	NP_776861.1	cGMP-dependent protein kinase 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKG1	NP_006249.1
Mus musculus (house mouse)	Prkg1	NP_001013855.1
Gallus gallus (chicken)	PRKG1	XP_003641507.2
	PRKG1	XP_001099460.1
Rattus norvegicus (Norway rat)	Prkg1	NP_001099201.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008863	XP_319605.4
Canis lupus familiaris (dog)	PRKG1	XP_851997.2
Bos taurus (cattle)	PRKG1	NP_776861.1
Danio rerio (zebrafish)	prkg1a	NP_957324.1
Drosophila melanogaster (fruit fly)	for	NP_477488.1
Xenopus tropicalis (tropical clawed frog)	LOC100494278	XP_002935474.2
Pan troglodytes (chimpanzee)	PRKG1	XP_001162858.1

Gene Ontology
Bibliography

Related articles in PubMed

Roles for cytosolic NADPH redox in regulating pulmonary artery relaxation by thiol oxidation-elicited subunit dimerization of protein kinase G1?.
Neo BH, Patel D, Kandhi S, Wolin MS
American journal of physiology. Heart and circulatory physiology305(3)H330-43(2013 Aug)

Roles for redox mechanisms controlling protein kinase G in pulmonary and coronary artery responses to hypoxia.
Neo BH, Kandhi S, Wolin MS
American journal of physiology. Heart and circulatory physiology301(6)H2295-304(2011 Dec)

Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication.
Osborne BW, Wu J, McFarland CJ, Nickl CK, Sankaran B, Casteel DE, Woods VL Jr, Kornev AP, Taylor SS, Dostmann WR
Structure (London, England : 1993)19(9)1317-27(2011 Sep)

Stabilization of cGMP-dependent protein kinase G (PKG) expression in vascular smooth muscle cells: contribution of 3'UTR of its mRNA.
Sellak H, Lincoln TM, Choi CS
Journal of biochemistry149(4)433-41(2011 Apr)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NADPH regulation of PKG1-alpha dimerization may contribute to vascular responses to hypoxia that are associated with changes in NADPH redox.
Title: Roles for cytosolic NADPH redox in regulating pulmonary artery relaxation by thiol oxidation-elicited subunit dimerization of protein kinase G1?.

Report roles for redox mechanisms controlling protein kinase G in pulmonary and coronary artery responses to hypoxia.
Title: Roles for redox mechanisms controlling protein kinase G in pulmonary and coronary artery responses to hypoxia.

Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication
Title: Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication.

The data suggest that PKG-I expression is subjected to post-transcriptional regulation in VSMC through the 3'UTR of its mRNA.
Title: Stabilization of cGMP-dependent protein kinase G (PKG) expression in vascular smooth muscle cells: contribution of 3'UTR of its mRNA.

cGMP binding destabilizes the N-terminus of cGMP-dependent protein kinase I alpha isozyme at the hinge region, centered around residue 77, while the C-terminus is protected from degradation.
Title: The hinge region operates as a stability switch in cGMP-dependent protein kinase I alpha.

The following PRKG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41779	NM_174436.2 Latest version!	Bos taurus protein kinase cGMP-dependent 1 (PRKG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa33435	XM_005225353.2 Latest version!	Bos taurus protein kinase, cGMP-dependent, type I (PRKG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa230728	XM_005225353.3 Latest version!	Bos taurus protein kinase cGMP-dependent 1 (PRKG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa230729	XM_024985478.1 Latest version!	Bos taurus protein kinase cGMP-dependent 1 (PRKG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKG1 ( NM_174436.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776861.1 Bos taurus protein kinase cGMP-dependent 1 (PRKG1), mRNA.

Price & Availability↑

CloneID	OBa41779
Clone ID Related Accession (Same CDS sequence)	NM_174436.2
Accession Version	NM_174436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	cGMP-dependent protein kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X16086.1. On or before Sep 25, 2006 this sequence version replaced XM_868336.1, NM_174436.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X16086.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGAGCGAGC TGGAGGAAGA CTTTGCCAAG ATTCTCATGC TCAAGGAGGA GAGGATCAAA 
GAGCTGGAGA AGCGGCTGTC AGAGAAGGAG GAAGAAATCC AGGAGCTGAA GAGGAAACTC 
CATAAATGCC AGTCAGTGCT GCCCGTGCCC TCGACCCACA TCGGCCCCCG GACCACCCGG 
GCACAGGGCA TCTCGGCCGA GCCGCAGACC TACAGGTCCT TCCACGACCT CCGACAGGCA 
TTCCGGAAGT TCACCAAATC CGAAAGGTCC AAGGATCTCA TAAAGGAGGC CATCCTTGAC 
AATGACTTTA TGAAGAACTT GGAGCTGTCA CAGATCCAAG AGATTGTGGA TTGTATGTAC 
CCAGTGGAGT ACGGCAAAGA CAGCTGCATC ATCAAAGAAG GAGATGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGTAA GGTTGAAGTT ACAAAAGAAG GCGTGAAGCT GTGCACAATG 
GGTCCTGGTA AAGTGTTTGG AGAGTTGGCT ATCCTTTACA ACTGTACCCG GACGGCGACC 
GTCAAAACTC TTGTAAATGT GAAACTCTGG GCCATTGATC GACAATGTTT TCAGACGATA 
ATGATGAGGA CAGGACTTAT CAAGCATACC GAGTATATGG AATTTTTAAA AAGCGTTCCA 
ACATTCCAGA GCCTTCCTGA AGAGATCCTC AGTAAGCTTG CTGACGTCCT TGAAGAGACC 
CACTATGAAA ATGGGGAATA TATCATCAGG CAAGGTGCAA GAGGGGACAC CTTCTTTATC 
ATCAGTAAAG GAAAGGTTAA TGTCACTCGT GAAGACTCGC CCAATGAAGA CCCAGTCTTT 
CTTAGAACCT TAGGAAAAGG AGATTGGTTT GGAGAGAAAG CCTTGCAGGG GGAAGATGTG 
AGAACAGCGA ATGTAATTGC GGCAGAAGCT GTAACCTGCC TTGTGATCGA CAGAGACTCT 
TTCAAACATT TGATTGGAGG ATTAGATGAT GTTTCTAATA AAGCATATGA AGATGCAGAA 
GCTAAGGCAA AATATGAAGC TGAAGCTGCT TTCTTCGCCA ACCTGAAGCT GTCTGATTTC 
AACATCATTG ACACCCTTGG AGTTGGAGGT TTCGGACGCG TAGAACTGGT CCAGTTAAAA 
AGTGAAGAAT CCAAAACCTT TGCAATGAAG ATTCTCAAGA AACGGCACAT CGTGGATACA 
AGACAGCAGG AACACATCCG CTCGGAGAAG CAGATCATGC AGGGGGCCCA TTCGGACTTC 
ATAGTGAGAT TATACAGAAC ATTTAAGGAC AGCAAATATT TGTATATGTT GATGGAAGCT 
TGCCTAGGTG GAGAGCTCTG GACCATTCTC AGGGATCGGG GGTCATTTGA AGATTCTACA 
ACCAGATTTT ATACAGCATG TGTGGTAGAA GCTTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC TGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGATTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACTCCAGAAT ATGTAGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCAGCCGAC 
TATTGGTCAC TGGGAATCCT CATGTATGAG CTTCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA TAACATCATA TTGAGGGGGA TTGACATGAT AGAGTTTCCA 
AAGAAGATTG CCAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA TAATCCATCA 
GAAAGATTAG GGAATTTGAA AAACGGAGTG AAAGACATTC AAAAGCACAA ATGGTTTGAG 
GGCTTTAATT GGGAAGGCTT AAGAAAAGGC ACCTTGACAC CTCCTATAAT ACCAAGTGTT 
GCATCACCCA CAGACACAAG CAATTTTGAC AGTTTCCCTG AGGACAATGA TGAACCGCCA 
CCTGATGACA ACTCAGGATG GGACATAGAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776861.1
CDS	7..2022
Translation	MSELEEDFAKILMLKEERIKELEKRLSEKEEEIQELKRKLHKCQSVLPVPSTHIGPRTTRAQGISAEPQTYRSFHDLRQAFRKFTKSERSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGKVNVTREDSPNEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	NM_174436.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cGMP-dependent 1 (PRKG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174436.2

ORF Insert Sequence:

ATGAGCGAGC TGGAGGAAGA CTTTGCCAAG ATTCTCATGC TCAAGGAGGA GAGGATCAAA 
GAGCTGGAGA AGCGGCTGTC AGAGAAGGAG GAAGAAATCC AGGAGCTGAA GAGGAAACTC 
CATAAATGCC AGTCAGTGCT GCCCGTGCCC TCGACCCACA TCGGCCCCCG GACCACCCGG 
GCACAGGGCA TCTCGGCCGA GCCGCAGACC TACAGGTCCT TCCACGACCT CCGACAGGCA 
TTCCGGAAGT TCACCAAATC CGAAAGGTCC AAGGATCTCA TAAAGGAGGC CATCCTTGAC 
AATGACTTTA TGAAGAACTT GGAGCTGTCA CAGATCCAAG AGATTGTGGA TTGTATGTAC 
CCAGTGGAGT ACGGCAAAGA CAGCTGCATC ATCAAAGAAG GAGATGTGGG GTCACTGGTG 
TATGTCATGG AAGATGGTAA GGTTGAAGTT ACAAAAGAAG GCGTGAAGCT GTGCACAATG 
GGTCCTGGTA AAGTGTTTGG AGAGTTGGCT ATCCTTTACA ACTGTACCCG GACGGCGACC 
GTCAAAACTC TTGTAAATGT GAAACTCTGG GCCATTGATC GACAATGTTT TCAGACGATA 
ATGATGAGGA CAGGACTTAT CAAGCATACC GAGTATATGG AATTTTTAAA AAGCGTTCCA 
ACATTCCAGA GCCTTCCTGA AGAGATCCTC AGTAAGCTTG CTGACGTCCT TGAAGAGACC 
CACTATGAAA ATGGGGAATA TATCATCAGG CAAGGTGCAA GAGGGGACAC CTTCTTTATC 
ATCAGTAAAG GAAAGGTTAA TGTCACTCGT GAAGACTCGC CCAATGAAGA CCCAGTCTTT 
CTTAGAACCT TAGGAAAAGG AGATTGGTTT GGAGAGAAAG CCTTGCAGGG GGAAGATGTG 
AGAACAGCGA ATGTAATTGC GGCAGAAGCT GTAACCTGCC TTGTGATCGA CAGAGACTCT 
TTCAAACATT TGATTGGAGG ATTAGATGAT GTTTCTAATA AAGCATATGA AGATGCAGAA 
GCTAAGGCAA AATATGAAGC TGAAGCTGCT TTCTTCGCCA ACCTGAAGCT GTCTGATTTC 
AACATCATTG ACACCCTTGG AGTTGGAGGT TTCGGACGCG TAGAACTGGT CCAGTTAAAA 
AGTGAAGAAT CCAAAACCTT TGCAATGAAG ATTCTCAAGA AACGGCACAT CGTGGATACA 
AGACAGCAGG AACACATCCG CTCGGAGAAG CAGATCATGC AGGGGGCCCA TTCGGACTTC 
ATAGTGAGAT TATACAGAAC ATTTAAGGAC AGCAAATATT TGTATATGTT GATGGAAGCT 
TGCCTAGGTG GAGAGCTCTG GACCATTCTC AGGGATCGGG GGTCATTTGA AGATTCTACA 
ACCAGATTTT ATACAGCATG TGTGGTAGAA GCTTTTGCCT ATCTGCATTC CAAAGGAATC 
ATTTACAGGG ACCTCAAGCC TGAAAATCTC ATCCTAGATC ACCGAGGTTA TGCCAAACTG 
GTTGATTTTG GCTTTGCAAA GAAAATAGGA TTTGGAAAGA AAACATGGAC TTTTTGTGGG 
ACTCCAGAAT ATGTAGCCCC AGAGATCATC CTGAACAAAG GCCATGACAT TTCAGCCGAC 
TATTGGTCAC TGGGAATCCT CATGTATGAG CTTCTGACTG GCAGCCCACC TTTCTCAGGC 
CCAGATCCTA TGAAAACCTA TAACATCATA TTGAGGGGGA TTGACATGAT AGAGTTTCCA 
AAGAAGATTG CCAAAAATGC TGCTAATTTA ATTAAAAAAC TATGCAGGGA TAATCCATCA 
GAAAGATTAG GGAATTTGAA AAACGGAGTG AAAGACATTC AAAAGCACAA ATGGTTTGAG 
GGCTTTAATT GGGAAGGCTT AAGAAAAGGC ACCTTGACAC CTCCTATAAT ACCAAGTGTT 
GCATCACCCA CAGACACAAG CAATTTTGAC AGTTTCCCTG AGGACAATGA TGAACCGCCA 
CCTGATGACA ACTCAGGATG GGACATAGAC TTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( XM_005225353.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225410.1 Bos taurus protein kinase, cGMP-dependent, type I (PRKG1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa33435
Clone ID Related Accession (Same CDS sequence)	XM_005225353.2
Accession Version	XM_005225353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	cGMP-dependent protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104580.1) and transcript sequence (BC110022.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 30, 2014 this sequence version replaced XM_005225353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 193 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGGCACCC TGCGGGATTT ACAGTATGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GGCCGGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCCC CTTGCAAGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCTGAGCCCA CCGCCTTTGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGTCCAC AGTCCAAGGA TCTCATAAAG GAGGCCATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCACAGAT CCAAGAGATT GTGGATTGTA TGTACCCAGT GGAGTACGGC 
AAAGACAGCT GCATCATCAA AGAAGGAGAT GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGCGTG AAGCTGTGCA CAATGGGTCC TGGTAAAGTG 
TTTGGAGAGT TGGCTATCCT TTACAACTGT ACCCGGACGG CGACCGTCAA AACTCTTGTA 
AATGTGAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAGA CGATAATGAT GAGGACAGGA 
CTTATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGTAA GCTTGCTGAC GTCCTTGAAG AGACCCACTA TGAAAATGGG 
GAATATATCA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG TAAAGGAAAG 
GTTAATGTCA CTCGTGAAGA CTCGCCCAAT GAAGACCCAG TCTTTCTTAG AACCTTAGGA 
AAAGGAGATT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCGAATGTA 
ATTGCGGCAG AAGCTGTAAC CTGCCTTGTG ATCGACAGAG ACTCTTTCAA ACATTTGATT 
GGAGGATTAG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAATAT 
GAAGCTGAAG CTGCTTTCTT CGCCAACCTG AAGCTGTCTG ATTTCAACAT CATTGACACC 
CTTGGAGTTG GAGGTTTCGG ACGAGTAGAA CTGGTCCAGT TAAAAAGTGA AGAATCCAAA 
ACCTTTGCAA TGAAGATTCT CAAGAAACGC CACATCGTGG ATACAAGACA GCAGGAACAC 
ATCCGCTCGG AGAAGCAGAT CATGCAGGGG GCCCATTCGG ACTTCATAGT GAGATTATAC 
AGAACATTTA AGGACAGCAA ATATTTGTAT ATGTTGATGG AAGCTTGCCT AGGTGGAGAG 
CTCTGGACCA TTCTCAGGGA TCGGGGGTCA TTTGAAGATT CTACAACCAG ATTTTATACA 
GCATGTGTGG TAGAAGCTTT TGCCTATCTG CATTCCAAAG GAATCATTTA CAGGGACCTC 
AAGCCTGAAA ATCTCATCCT AGATCACCGA GGTTATGCCA AACTGGTTGA TTTTGGCTTT 
GCAAAGAAAA TAGGATTTGG AAAGAAAACA TGGACTTTTT GTGGGACTCC AGAATATGTA 
GCCCCAGAGA TCATCCTGAA CAAAGGCCAT GACATTTCAG CCGACTATTG GTCACTGGGA 
ATCCTCATGT ATGAGCTTCT GACTGGCAGC CCACCTTTCT CAGGCCCAGA TCCTATGAAA 
ACCTATAACA TCATATTGAG GGGGATTGAC ATGATAGAGT TTCCAAAGAA GATTGCCAAA 
AATGCTGCTA ATTTAATTAA AAAACTATGC AGGGATAATC CATCAGAAAG ATTAGGGAAT 
TTGAAAAACG GAGTGAAAGA CATTCAAAAG CACAAATGGT TTGAGGGCTT TAATTGGGAA 
GGCTTAAGAA AAGGCACCTT GACACCTCCT ATAATACCAA GTGTTGCATC ACCCACAGAC 
ACAAGCAATT TTGACAGTTT CCCTGAGGAC AATGACGAAC CGCCACCTGA TGACAACTCA 
GGATGGGACA TAGACTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225410.1
CDS	342..2402
Translation	MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQAQKQSASPLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGKVNVTREDSPNEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	XM_005225353.2
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase, cGMP-dependent, type I (PRKG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225353.2

ORF Insert Sequence:

ATGGGCACCC TGCGGGATTT ACAGTATGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GGCCGGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCCC CTTGCAAGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCTGAGCCCA CCGCCTTTGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGTCCAC AGTCCAAGGA TCTCATAAAG GAGGCCATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCACAGAT CCAAGAGATT GTGGATTGTA TGTACCCAGT GGAGTACGGC 
AAAGACAGCT GCATCATCAA AGAAGGAGAT GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGCGTG AAGCTGTGCA CAATGGGTCC TGGTAAAGTG 
TTTGGAGAGT TGGCTATCCT TTACAACTGT ACCCGGACGG CGACCGTCAA AACTCTTGTA 
AATGTGAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAGA CGATAATGAT GAGGACAGGA 
CTTATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGTAA GCTTGCTGAC GTCCTTGAAG AGACCCACTA TGAAAATGGG 
GAATATATCA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG TAAAGGAAAG 
GTTAATGTCA CTCGTGAAGA CTCGCCCAAT GAAGACCCAG TCTTTCTTAG AACCTTAGGA 
AAAGGAGATT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCGAATGTA 
ATTGCGGCAG AAGCTGTAAC CTGCCTTGTG ATCGACAGAG ACTCTTTCAA ACATTTGATT 
GGAGGATTAG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAATAT 
GAAGCTGAAG CTGCTTTCTT CGCCAACCTG AAGCTGTCTG ATTTCAACAT CATTGACACC 
CTTGGAGTTG GAGGTTTCGG ACGAGTAGAA CTGGTCCAGT TAAAAAGTGA AGAATCCAAA 
ACCTTTGCAA TGAAGATTCT CAAGAAACGC CACATCGTGG ATACAAGACA GCAGGAACAC 
ATCCGCTCGG AGAAGCAGAT CATGCAGGGG GCCCATTCGG ACTTCATAGT GAGATTATAC 
AGAACATTTA AGGACAGCAA ATATTTGTAT ATGTTGATGG AAGCTTGCCT AGGTGGAGAG 
CTCTGGACCA TTCTCAGGGA TCGGGGGTCA TTTGAAGATT CTACAACCAG ATTTTATACA 
GCATGTGTGG TAGAAGCTTT TGCCTATCTG CATTCCAAAG GAATCATTTA CAGGGACCTC 
AAGCCTGAAA ATCTCATCCT AGATCACCGA GGTTATGCCA AACTGGTTGA TTTTGGCTTT 
GCAAAGAAAA TAGGATTTGG AAAGAAAACA TGGACTTTTT GTGGGACTCC AGAATATGTA 
GCCCCAGAGA TCATCCTGAA CAAAGGCCAT GACATTTCAG CCGACTATTG GTCACTGGGA 
ATCCTCATGT ATGAGCTTCT GACTGGCAGC CCACCTTTCT CAGGCCCAGA TCCTATGAAA 
ACCTATAACA TCATATTGAG GGGGATTGAC ATGATAGAGT TTCCAAAGAA GATTGCCAAA 
AATGCTGCTA ATTTAATTAA AAAACTATGC AGGGATAATC CATCAGAAAG ATTAGGGAAT 
TTGAAAAACG GAGTGAAAGA CATTCAAAAG CACAAATGGT TTGAGGGCTT TAATTGGGAA 
GGCTTAAGAA AAGGCACCTT GACACCTCCT ATAATACCAA GTGTTGCATC ACCCACAGAC 
ACAAGCAATT TTGACAGTTT CCCTGAGGAC AATGACGAAC CGCCACCTGA TGACAACTCA 
GGATGGGACA TAGACTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( XM_005225353.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005225410.2 Bos taurus protein kinase cGMP-dependent 1 (PRKG1), transcript variant X1, mRNA.

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CloneID	OBa230728
Clone ID Related Accession (Same CDS sequence)	XM_005225353.3
Accession Version	XM_005225353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCC TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GGCCGGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAAGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCTGAGCCCA CCGCCTTTGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGTCCAC AGTCCAAGGA TCTCATAAAG GAGGCCATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCACAGAT CCAAGAGATT GTGGATTGTA TGTACCCAGT GGAGTACGGC 
AAAGACAGCT GCATCATCAA AGAAGGAGAT GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGCGTG AAGCTGTGCA CAATGGGTCC TGGTAAAGTG 
TTTGGAGAGT TGGCTATCCT TTACAACTGT ACCCGGACGG CGACCGTCAA AACTCTTGTA 
AATGTGAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAGA CGATAATGAT GAGGACAGGA 
CTTATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGTAA GCTTGCTGAC GTCCTTGAAG AGACCCACTA TGAAAATGGG 
GAATATATCA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG TAAAGGAAAG 
GTTAATGTCA CTCGTGAAGA CTCGCCCAAT GAAGACCCAG TCTTTCTTAG AACCTTAGGA 
AAAGGAGATT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCGAATGTA 
ATTGCGGCAG AAGCTGTAAC CTGCCTTGTG ATCGACAGAG ACTCTTTCAA ACATTTGATT 
GGAGGATTAG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAATAT 
GAAGCTGAAG CTGCTTTCTT CGCCAACCTG AAGCTGTCTG ATTTCAACAT CATTGACACC 
CTTGGAGTTG GAGGTTTCGG ACGAGTAGAA CTGGTCCAGT TAAAAAGTGA AGAATCCAAA 
ACCTTTGCAA TGAAGATTCT CAAGAAACGC CACATCGTGG ATACAAGACA GCAGGAACAC 
ATCCGCTCGG AGAAGCAGAT CATGCAGGGG GCCCATTCGG ACTTCATAGT GAGATTATAC 
AGAACATTTA AGGACAGCAA ATATTTGTAT ATGTTGATGG AAGCTTGCCT AGGTGGAGAG 
CTCTGGACCA TTCTCAGGGA TCGGGGGTCA TTTGAAGATT CTACAACCAG ATTTTATACA 
GCATGTGTGG TAGAAGCTTT TGCCTATCTG CATTCCAAAG GAATCATTTA CAGGGACCTC 
AAGCCTGAAA ATCTCATCCT AGATCACCGA GGTTATGCCA AACTGGTTGA TTTTGGCTTT 
GCAAAGAAAA TAGGATTTGG AAAGAAAACA TGGACTTTTT GTGGGACTCC AGAATATGTA 
GCCCCAGAGA TCATCCTGAA CAAAGGCCAT GACATTTCAG CCGACTATTG GTCACTGGGA 
ATCCTCATGT ATGAGCTTCT GACTGGCAGC CCACCTTTCT CAGGCCCAGA TCCTATGAAA 
ACCTATAACA TCATATTGAG GGGGATTGAC ATGATAGAGT TTCCAAAGAA GATTGCCAAA 
AATGCTGCTA ATTTAATTAA AAAACTATGC AGGGATAATC CATCAGAAAG ATTAGGGAAT 
TTGAAAAACG GAGTGAAAGA CATTCAAAAG CACAAATGGT TTGAGGGCTT TAATTGGGAA 
GGCTTAAGAA AAGGCACCTT GACACCTCCT ATAATACCAA GTGTTGCATC ACCCACAGAC 
ACAAGCAATT TTGACAGTTT CCCTGAGGAC AATGACGAAC CGCCACCTGA TGACAACTCA 
GGATGGGACA TAGACTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005225410.2
CDS	421..2481
Translation	MGTLRDLQYALQEKIEELRQRDALIDELELELDQKDELIQKLQNELDKYRSVIRPATQQAQKQSASTLQGEPRTKRQAISAEPTAFDIQDLSHVTLPFYPKSPQSKDLIKEAILDNDFMKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGKVNVTREDSPNEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	XM_005225353.3
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cGMP-dependent 1 (PRKG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005225353.3

ORF Insert Sequence:

ATGGGCACCC TGCGGGATTT ACAGTACGCG CTCCAGGAGA AGATCGAGGA GCTGAGGCAG 
CGGGATGCTC TCATCGACGA GCTGGAGCTG GAGTTGGATC AGAAGGACGA ACTGATCCAG 
AAGCTGCAGA ACGAGCTGGA CAAGTACCGC TCGGTGATCC GGCCGGCCAC CCAGCAGGCG 
CAGAAGCAGA GCGCGAGCAC CTTGCAAGGC GAGCCGCGCA CCAAGCGGCA GGCGATCTCC 
GCTGAGCCCA CCGCCTTTGA CATCCAGGAT CTCAGCCATG TGACCCTGCC CTTCTACCCC 
AAGAGTCCAC AGTCCAAGGA TCTCATAAAG GAGGCCATCC TTGACAATGA CTTTATGAAG 
AACTTGGAGC TGTCACAGAT CCAAGAGATT GTGGATTGTA TGTACCCAGT GGAGTACGGC 
AAAGACAGCT GCATCATCAA AGAAGGAGAT GTGGGGTCAC TGGTGTATGT CATGGAAGAT 
GGTAAGGTTG AAGTTACAAA AGAAGGCGTG AAGCTGTGCA CAATGGGTCC TGGTAAAGTG 
TTTGGAGAGT TGGCTATCCT TTACAACTGT ACCCGGACGG CGACCGTCAA AACTCTTGTA 
AATGTGAAAC TCTGGGCCAT TGATCGACAA TGTTTTCAGA CGATAATGAT GAGGACAGGA 
CTTATCAAGC ATACCGAGTA TATGGAATTT TTAAAAAGCG TTCCAACATT CCAGAGCCTT 
CCTGAAGAGA TCCTCAGTAA GCTTGCTGAC GTCCTTGAAG AGACCCACTA TGAAAATGGG 
GAATATATCA TCAGGCAAGG TGCAAGAGGG GACACCTTCT TTATCATCAG TAAAGGAAAG 
GTTAATGTCA CTCGTGAAGA CTCGCCCAAT GAAGACCCAG TCTTTCTTAG AACCTTAGGA 
AAAGGAGATT GGTTTGGAGA GAAAGCCTTG CAGGGGGAAG ATGTGAGAAC AGCGAATGTA 
ATTGCGGCAG AAGCTGTAAC CTGCCTTGTG ATCGACAGAG ACTCTTTCAA ACATTTGATT 
GGAGGATTAG ATGATGTTTC TAATAAAGCA TATGAAGATG CAGAAGCTAA AGCAAAATAT 
GAAGCTGAAG CTGCTTTCTT CGCCAACCTG AAGCTGTCTG ATTTCAACAT CATTGACACC 
CTTGGAGTTG GAGGTTTCGG ACGAGTAGAA CTGGTCCAGT TAAAAAGTGA AGAATCCAAA 
ACCTTTGCAA TGAAGATTCT CAAGAAACGC CACATCGTGG ATACAAGACA GCAGGAACAC 
ATCCGCTCGG AGAAGCAGAT CATGCAGGGG GCCCATTCGG ACTTCATAGT GAGATTATAC 
AGAACATTTA AGGACAGCAA ATATTTGTAT ATGTTGATGG AAGCTTGCCT AGGTGGAGAG 
CTCTGGACCA TTCTCAGGGA TCGGGGGTCA TTTGAAGATT CTACAACCAG ATTTTATACA 
GCATGTGTGG TAGAAGCTTT TGCCTATCTG CATTCCAAAG GAATCATTTA CAGGGACCTC 
AAGCCTGAAA ATCTCATCCT AGATCACCGA GGTTATGCCA AACTGGTTGA TTTTGGCTTT 
GCAAAGAAAA TAGGATTTGG AAAGAAAACA TGGACTTTTT GTGGGACTCC AGAATATGTA 
GCCCCAGAGA TCATCCTGAA CAAAGGCCAT GACATTTCAG CCGACTATTG GTCACTGGGA 
ATCCTCATGT ATGAGCTTCT GACTGGCAGC CCACCTTTCT CAGGCCCAGA TCCTATGAAA 
ACCTATAACA TCATATTGAG GGGGATTGAC ATGATAGAGT TTCCAAAGAA GATTGCCAAA 
AATGCTGCTA ATTTAATTAA AAAACTATGC AGGGATAATC CATCAGAAAG ATTAGGGAAT 
TTGAAAAACG GAGTGAAAGA CATTCAAAAG CACAAATGGT TTGAGGGCTT TAATTGGGAA 
GGCTTAAGAA AAGGCACCTT GACACCTCCT ATAATACCAA GTGTTGCATC ACCCACAGAC 
ACAAGCAATT TTGACAGTTT CCCTGAGGAC AATGACGAAC CGCCACCTGA TGACAACTCA 
GGATGGGACA TAGACTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKG1 ( XM_024985478.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841246.1 Bos taurus protein kinase cGMP-dependent 1 (PRKG1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa230729
Clone ID Related Accession (Same CDS sequence)	XM_024985478.1
Accession Version	XM_024985478.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cGMP-dependent protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACT TGGAGCTGTC ACAGATCCAA GAGATTGTGG ATTGTATGTA CCCAGTGGAG 
TACGGCAAAG ACAGCTGCAT CATCAAAGAA GGAGATGTGG GGTCACTGGT GTATGTCATG 
GAAGATGGTA AGGTTGAAGT TACAAAAGAA GGCGTGAAGC TGTGCACAAT GGGTCCTGGT 
AAAGTGTTTG GAGAGTTGGC TATCCTTTAC AACTGTACCC GGACGGCGAC CGTCAAAACT 
CTTGTAAATG TGAAACTCTG GGCCATTGAT CGACAATGTT TTCAGACGAT AATGATGAGG 
ACAGGACTTA TCAAGCATAC CGAGTATATG GAATTTTTAA AAAGCGTTCC AACATTCCAG 
AGCCTTCCTG AAGAGATCCT CAGTAAGCTT GCTGACGTCC TTGAAGAGAC CCACTATGAA 
AATGGGGAAT ATATCATCAG GCAAGGTGCA AGAGGGGACA CCTTCTTTAT CATCAGTAAA 
GGAAAGGTTA ATGTCACTCG TGAAGACTCG CCCAATGAAG ACCCAGTCTT TCTTAGAACC 
TTAGGAAAAG GAGATTGGTT TGGAGAGAAA GCCTTGCAGG GGGAAGATGT GAGAACAGCG 
AATGTAATTG CGGCAGAAGC TGTAACCTGC CTTGTGATCG ACAGAGACTC TTTCAAACAT 
TTGATTGGAG GATTAGATGA TGTTTCTAAT AAAGCATATG AAGATGCAGA AGCTAAAGCA 
AAATATGAAG CTGAAGCTGC TTTCTTCGCC AACCTGAAGC TGTCTGATTT CAACATCATT 
GACACCCTTG GAGTTGGAGG TTTCGGACGA GTAGAACTGG TCCAGTTAAA AAGTGAAGAA 
TCCAAAACCT TTGCAATGAA GATTCTCAAG AAACGCCACA TCGTGGATAC AAGACAGCAG 
GAACACATCC GCTCGGAGAA GCAGATCATG CAGGGGGCCC ATTCGGACTT CATAGTGAGA 
TTATACAGAA CATTTAAGGA CAGCAAATAT TTGTATATGT TGATGGAAGC TTGCCTAGGT 
GGAGAGCTCT GGACCATTCT CAGGGATCGG GGGTCATTTG AAGATTCTAC AACCAGATTT 
TATACAGCAT GTGTGGTAGA AGCTTTTGCC TATCTGCATT CCAAAGGAAT CATTTACAGG 
GACCTCAAGC CTGAAAATCT CATCCTAGAT CACCGAGGTT ATGCCAAACT GGTTGATTTT 
GGCTTTGCAA AGAAAATAGG ATTTGGAAAG AAAACATGGA CTTTTTGTGG GACTCCAGAA 
TATGTAGCCC CAGAGATCAT CCTGAACAAA GGCCATGACA TTTCAGCCGA CTATTGGTCA 
CTGGGAATCC TCATGTATGA GCTTCTGACT GGCAGCCCAC CTTTCTCAGG CCCAGATCCT 
ATGAAAACCT ATAACATCAT ATTGAGGGGG ATTGACATGA TAGAGTTTCC AAAGAAGATT 
GCCAAAAATG CTGCTAATTT AATTAAAAAA CTATGCAGGG ATAATCCATC AGAAAGATTA 
GGGAATTTGA AAAACGGAGT GAAAGACATT CAAAAGCACA AATGGTTTGA GGGCTTTAAT 
TGGGAAGGCT TAAGAAAAGG CACCTTGACA CCTCCTATAA TACCAAGTGT TGCATCACCC 
ACAGACACAA GCAATTTTGA CAGTTTCCCT GAGGACAATG ACGAACCGCC ACCTGATGAC 
AACTCAGGAT GGGACATAGA CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841246.1
CDS	493..2199
Translation	MKNLELSQIQEIVDCMYPVEYGKDSCIIKEGDVGSLVYVMEDGKVEVTKEGVKLCTMGPGKVFGELAILYNCTRTATVKTLVNVKLWAIDRQCFQTIMMRTGLIKHTEYMEFLKSVPTFQSLPEEILSKLADVLEETHYENGEYIIRQGARGDTFFIISKGKVNVTREDSPNEDPVFLRTLGKGDWFGEKALQGEDVRTANVIAAEAVTCLVIDRDSFKHLIGGLDDVSNKAYEDAEAKAKYEAEAAFFANLKLSDFNIIDTLGVGGFGRVELVQLKSEESKTFAMKILKKRHIVDTRQQEHIRSEKQIMQGAHSDFIVRLYRTFKDSKYLYMLMEACLGGELWTILRDRGSFEDSTTRFYTACVVEAFAYLHSKGIIYRDLKPENLILDHRGYAKLVDFGFAKKIGFGKKTWTFCGTPEYVAPEIILNKGHDISADYWSLGILMYELLTGSPPFSGPDPMKTYNIILRGIDMIEFPKKIAKNAANLIKKLCRDNPSERLGNLKNGVKDIQKHKWFEGFNWEGLRKGTLTPPIIPSVASPTDTSNFDSFPEDNDEPPPDDNSGWDIDF

Target ORF information:

RefSeq Version	XM_024985478.1
Organism	Bos taurus(cattle)
Definition	Bos taurus protein kinase cGMP-dependent 1 (PRKG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024985478.1

ORF Insert Sequence:

ATGAAGAACT TGGAGCTGTC ACAGATCCAA GAGATTGTGG ATTGTATGTA CCCAGTGGAG 
TACGGCAAAG ACAGCTGCAT CATCAAAGAA GGAGATGTGG GGTCACTGGT GTATGTCATG 
GAAGATGGTA AGGTTGAAGT TACAAAAGAA GGCGTGAAGC TGTGCACAAT GGGTCCTGGT 
AAAGTGTTTG GAGAGTTGGC TATCCTTTAC AACTGTACCC GGACGGCGAC CGTCAAAACT 
CTTGTAAATG TGAAACTCTG GGCCATTGAT CGACAATGTT TTCAGACGAT AATGATGAGG 
ACAGGACTTA TCAAGCATAC CGAGTATATG GAATTTTTAA AAAGCGTTCC AACATTCCAG 
AGCCTTCCTG AAGAGATCCT CAGTAAGCTT GCTGACGTCC TTGAAGAGAC CCACTATGAA 
AATGGGGAAT ATATCATCAG GCAAGGTGCA AGAGGGGACA CCTTCTTTAT CATCAGTAAA 
GGAAAGGTTA ATGTCACTCG TGAAGACTCG CCCAATGAAG ACCCAGTCTT TCTTAGAACC 
TTAGGAAAAG GAGATTGGTT TGGAGAGAAA GCCTTGCAGG GGGAAGATGT GAGAACAGCG 
AATGTAATTG CGGCAGAAGC TGTAACCTGC CTTGTGATCG ACAGAGACTC TTTCAAACAT 
TTGATTGGAG GATTAGATGA TGTTTCTAAT AAAGCATATG AAGATGCAGA AGCTAAAGCA 
AAATATGAAG CTGAAGCTGC TTTCTTCGCC AACCTGAAGC TGTCTGATTT CAACATCATT 
GACACCCTTG GAGTTGGAGG TTTCGGACGA GTAGAACTGG TCCAGTTAAA AAGTGAAGAA 
TCCAAAACCT TTGCAATGAA GATTCTCAAG AAACGCCACA TCGTGGATAC AAGACAGCAG 
GAACACATCC GCTCGGAGAA GCAGATCATG CAGGGGGCCC ATTCGGACTT CATAGTGAGA 
TTATACAGAA CATTTAAGGA CAGCAAATAT TTGTATATGT TGATGGAAGC TTGCCTAGGT 
GGAGAGCTCT GGACCATTCT CAGGGATCGG GGGTCATTTG AAGATTCTAC AACCAGATTT 
TATACAGCAT GTGTGGTAGA AGCTTTTGCC TATCTGCATT CCAAAGGAAT CATTTACAGG 
GACCTCAAGC CTGAAAATCT CATCCTAGAT CACCGAGGTT ATGCCAAACT GGTTGATTTT 
GGCTTTGCAA AGAAAATAGG ATTTGGAAAG AAAACATGGA CTTTTTGTGG GACTCCAGAA 
TATGTAGCCC CAGAGATCAT CCTGAACAAA GGCCATGACA TTTCAGCCGA CTATTGGTCA 
CTGGGAATCC TCATGTATGA GCTTCTGACT GGCAGCCCAC CTTTCTCAGG CCCAGATCCT 
ATGAAAACCT ATAACATCAT ATTGAGGGGG ATTGACATGA TAGAGTTTCC AAAGAAGATT 
GCCAAAAATG CTGCTAATTT AATTAAAAAA CTATGCAGGG ATAATCCATC AGAAAGATTA 
GGGAATTTGA AAAACGGAGT GAAAGACATT CAAAAGCACA AATGGTTTGA GGGCTTTAAT 
TGGGAAGGCT TAAGAAAAGG CACCTTGACA CCTCCTATAA TACCAAGTGT TGCATCACCC 
ACAGACACAA GCAATTTTGA CAGTTTCCCT GAGGACAATG ACGAACCGCC ACCTGATGAC 
AACTCAGGAT GGGACATAGA CTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Roles for cytosolic NADPH redox in regulating pulmonary artery relaxation by thiol oxidation-elicited subunit dimerization of protein kinase G1?.
American journal of physiology. Heart and circulatory physiology305(3)H330-43(2013 Aug)
Neo BH,Patel D,Kandhi S,Wolin MS

Roles for redox mechanisms controlling protein kinase G in pulmonary and coronary artery responses to hypoxia.
American journal of physiology. Heart and circulatory physiology301(6)H2295-304(2011 Dec)
Neo BH,Kandhi S,Wolin MS

Crystal structure of cGMP-dependent protein kinase reveals novel site of interchain communication.
Structure (London, England : 1993)19(9)1317-27(2011 Sep)
Osborne BW,Wu J,McFarland CJ,Nickl CK,Sankaran B,Casteel DE,Woods VL Jr,Kornev AP,Taylor SS,Dostmann WR

Stabilization of cGMP-dependent protein kinase G (PKG) expression in vascular smooth muscle cells: contribution of 3'UTR of its mRNA.
Journal of biochemistry149(4)433-41(2011 Apr)
Sellak H,Lincoln TM,Choi CS

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PRKG1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム