PDE6G cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE6G
Entrez Gene ID	281977
Full Name	phosphodiesterase 6G
General protein information	Preferred Names phosphodiesterase 6G Names retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma GMP-PDE gamma phosphodiesterase 6G, cGMP-specific, rod, gamma phosphodiesterase, cyclic GMP (rod receptor), gamma polypeptide
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010816449.1	XP_010814751.1	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
XM_010816448.2	XP_010814750.1	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
NM_174421.2	NP_776846.1	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
XM_010816448.3	XP_010814750.1	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
XM_024979612.1	XP_024835380.1	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	pde6g	NP_001165049.1
Homo sapiens (human)	PDE6G	NP_002593.1
Canis lupus familiaris (dog)	PDE6G	NP_001003235.1
Mus musculus (house mouse)	Pde6g	NP_036197.1
Gallus gallus (chicken)	PDE6G	NP_989776.1
Rattus norvegicus (Norway rat)	Pde6g	XP_003751031.1
	LOC719052	XP_001112634.2
Bos taurus (cattle)	PDE6G	NP_776846.1
Danio rerio (zebrafish)	pde6g	NP_997964.1
Xenopus tropicalis (tropical clawed frog)	LOC101732382	XP_004916403.1

Gene Ontology
Bibliography

Related articles in PubMed

Domain organization and conformational plasticity of the G protein effector, PDE6.
Zhang Z, He F, Constantine R, Baker ML, Baehr W, Schmid MF, Wensel TG, Agosto MA
The Journal of biological chemistry290(20)12833-43(2015 May)

Structural characterization of the rod cGMP phosphodiesterase 6.
Goc A, Chami M, Lodowski DT, Bosshart P, Moiseenkova-Bell V, Baehr W, Engel A, Palczewski K
Journal of molecular biology401(3)363-73(2010 Aug)

Structural requirements of the photoreceptor phosphodiesterase gamma-subunit for inhibition of rod PDE6 holoenzyme and for its activation by transducin.
Zhang XJ, Skiba NP, Cote RH
The Journal of biological chemistry285(7)4455-63(2010 Feb)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The approximately 11 A map revealed previously unseen features of PDE6, and each domain was readily fit with high resolution structures.
Title: Domain organization and conformational plasticity of the G protein effector, PDE6.

The 3D structure of the PDE6alphabetagamma complex was determined up to 18 A resolution from single-particle projections and was interpreted by model building to identify the probable location of isoprenylation, PDE6gamma subunits, and catalytic site
Title: Structural characterization of the rod cGMP phosphodiesterase 6.

Transducin activation of PDE6 appears to require interaction with both the C terminus and other regions of Pgamma to effectively relieve its inhibitory constraint.
Title: Structural requirements of the photoreceptor phosphodiesterase gamma-subunit for inhibition of rod PDE6 holoenzyme and for its activation by transducin.

although Pgamma-mediated regulation plays the dominant role in visual excitation, the direct, inter-domain allosteric regulation may play a feedback role in light adaptational processes during phototransduction
Title: Direct allosteric regulation between the GAF domain and catalytic domain of photoreceptor phosphodiesterase PDE6.

The Pgamma N-terminal Val16-Phe30 region was found to interact with the Palphabeta GAFa domain, whereas its C terminus (Phe73-Ile87) interacted with the Palphabeta catalytic domain.
Title: The inhibitory gamma subunit of the rod cGMP phosphodiesterase binds the catalytic subunits in an extended linear structure.

The following PDE6G gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE6G cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa30217	NM_174421.2 Latest version!	Bos taurus phosphodiesterase 6G (PDE6G), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa30217	XM_024979612.1 Latest version!	Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa30217	XM_010816449.1 Latest version!	Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa30217	XM_010816448.3 Latest version!	Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OBa30217	XM_010816448.2	Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE6G ( NM_174421.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776846.1 Bos taurus phosphodiesterase 6G (PDE6G), mRNA.

Price & Availability↑

CloneID	OBa30217
Clone ID Related Accession (Same CDS sequence)	XM_010816449.1 , XM_010816448.2 , NM_174421.2 , XM_010816448.3 , XM_024979612.1
Accession Version	NM_174421.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X04270.1. On Jun 3, 2003 this sequence version replaced NM_174421.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X04270.1, X04823.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145426 [ECO:0000348] ##Evidence-Data-END##

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776846.1
CDS	55..318
Translation	MNLEPPKAEIRSATRVMGGPVTPRKGPPKFKQRQTRQFKSKPPKKGVQGFGDDIPGMEGLGTDITVICPWEAFNHLELHELAQYGII

Target ORF information:

RefSeq Version	NM_174421.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 6G (PDE6G), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174421.2

ORF Insert Sequence:

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6G ( XM_010816449.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814751.1 Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30217
Clone ID Related Accession (Same CDS sequence)	XM_010816449.1 , XM_010816448.2 , NM_174421.2 , XM_010816448.3 , XM_024979612.1
Accession Version	XM_010816449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104498.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814751.1
CDS	183..446
Translation	MNLEPPKAEIRSATRVMGGPVTPRKGPPKFKQRQTRQFKSKPPKKGVQGFGDDIPGMEGLGTDITVICPWEAFNHLELHELAQYGII

Target ORF information:

RefSeq Version	XM_010816449.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816449.1

ORF Insert Sequence:

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6G ( XM_010816448.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814750.1 Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30217
Clone ID Related Accession (Same CDS sequence)	XM_010816449.1 , XM_010816448.2 , NM_174421.2 , XM_010816448.3 , XM_024979612.1
Accession Version	XM_010816448.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104498.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010816448.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814750.1
CDS	166..429
Translation	MNLEPPKAEIRSATRVMGGPVTPRKGPPKFKQRQTRQFKSKPPKKGVQGFGDDIPGMEGLGTDITVICPWEAFNHLELHELAQYGII

Target ORF information:

RefSeq Version	XM_010816448.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816448.2

ORF Insert Sequence:

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6G ( XM_010816448.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010814750.1 Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30217
Clone ID Related Accession (Same CDS sequence)	XM_010816449.1 , XM_010816448.2 , NM_174421.2 , XM_010816448.3 , XM_024979612.1
Accession Version	XM_010816448.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010816448.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010814750.1
CDS	268..531
Translation	MNLEPPKAEIRSATRVMGGPVTPRKGPPKFKQRQTRQFKSKPPKKGVQGFGDDIPGMEGLGTDITVICPWEAFNHLELHELAQYGII

Target ORF information:

RefSeq Version	XM_010816448.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010816448.3

ORF Insert Sequence:

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE6G ( XM_024979612.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835380.1 Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30217
Clone ID Related Accession (Same CDS sequence)	XM_010816449.1 , XM_010816448.2 , NM_174421.2 , XM_010816448.3 , XM_024979612.1
Accession Version	XM_024979612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit gamma isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835380.1
CDS	205..468
Translation	MNLEPPKAEIRSATRVMGGPVTPRKGPPKFKQRQTRQFKSKPPKKGVQGFGDDIPGMEGLGTDITVICPWEAFNHLELHELAQYGII

Target ORF information:

RefSeq Version	XM_024979612.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphodiesterase 6G (PDE6G), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979612.1

ORF Insert Sequence:

ATGAACCTGG AGCCACCCAA GGCCGAGATC CGGTCGGCCA CCAGGGTGAT GGGGGGACCC 
GTCACTCCCA GGAAAGGGCC CCCGAAATTT AAGCAGCGGC AAACCAGGCA GTTCAAGAGC 
AAGCCCCCCA AGAAAGGTGT CCAAGGGTTT GGTGATGACA TCCCTGGAAT GGAAGGCCTG 
GGAACAGACA TCACCGTCAT CTGCCCGTGG GAGGCCTTCA ACCACCTGGA GCTGCACGAG 
CTGGCCCAGT ACGGCATCAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Domain organization and conformational plasticity of the G protein effector, PDE6.
The Journal of biological chemistry290(20)12833-43(2015 May)
Zhang Z,He F,Constantine R,Baker ML,Baehr W,Schmid MF,Wensel TG,Agosto MA

Structural characterization of the rod cGMP phosphodiesterase 6.
Journal of molecular biology401(3)363-73(2010 Aug)
Goc A,Chami M,Lodowski DT,Bosshart P,Moiseenkova-Bell V,Baehr W,Engel A,Palczewski K

Structural requirements of the photoreceptor phosphodiesterase gamma-subunit for inhibition of rod PDE6 holoenzyme and for its activation by transducin.
The Journal of biological chemistry285(7)4455-63(2010 Feb)
Zhang XJ,Skiba NP,Cote RH

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PDE6G cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

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電話

FAX

オンラインフォーム