ABCA4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ABCA4
Entrez Gene ID	281584
Full Name	ATP binding cassette subfamily A member 4
General protein information	Preferred Names ATP binding cassette subfamily A member 4 Names retinal-specific ATP-binding cassette transporter ATP-binding cassette, sub-family A (ABC1), member 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010803293.2	XP_010801595.1	retinal-specific ATP-binding cassette transporter isoform X1
NM_174221.2	NP_776646.1	retinal-specific ATP-binding cassette transporter

Pathways from BioSystems

Homologies

Homo sapiens (human)	ABCA4	NP_000341.2
Canis lupus familiaris (dog)	ABCA4	NP_001003360.2
Mus musculus (house mouse)	Abca4	NP_031404.1
Pan troglodytes (chimpanzee)	ABCA4	XP_001152577.2
Gallus gallus (chicken)	ABCA4	XP_422330.3
Danio rerio (zebrafish)	abca4b	XP_683123.5
Xenopus tropicalis (tropical clawed frog)	abca4	NP_001011033.1
	ABCA4	XP_002808277.1
Rattus norvegicus (Norway rat)	Abca4	NP_001101191.1
Bos taurus (cattle)	ABCA4	NP_776646.1

Gene Ontology
Bibliography

Related articles in PubMed

ATP-binding cassette transporter ABCA4 and chemical isomerization protect photoreceptor cells from the toxic accumulation of excess 11-cis-retinal.
Quazi F, Molday RS
Proceedings of the National Academy of Sciences of the United States of America111(13)5024-9(2014 Apr)

Molecular organization and ATP-induced conformational changes of ABCA4, the photoreceptor-specific ABC transporter.
Tsybovsky Y, Orban T, Molday RS, Taylor D, Palczewski K
Structure (London, England : 1993)21(5)854-60(2013 May)

Posttranslational modifications of the photoreceptor-specific ABC transporter ABCA4.
Tsybovsky Y, Wang B, Quazi F, Molday RS, Palczewski K
Biochemistry50(32)6855-66(2011 Aug)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

N-retinylidene-phosphatidylethanolamine is the preferred retinoid substrate for the photoreceptor-specific ABC transporter ABCA4 (ABCR).
Beharry S, Zhong M, Molday RS
The Journal of biological chemistry279(52)53972-9(2004 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ABCA4 can transport N-11-cis-retinylidene-phosphatidylethanolamine (PE), the Schiff-base conjugate of 11-cis-retinal and PE, from the lumen to the cytoplasmic leaflet of disk membranes.
Title: ATP-binding cassette transporter ABCA4 and chemical isomerization protect photoreceptor cells from the toxic accumulation of excess 11-cis-retinal.

An 18 A-resolution structure of ABCA4 isolated from bovine rod outer segments was determined using electron microscopy and single-particle reconstruction
Title: Molecular organization and ATP-induced conformational changes of ABCA4, the photoreceptor-specific ABC transporter.

partial dephosphorylation of native bovine ABCA4 led to reduction of both basal and stimulated ATPase activity. Thus, we present the first evidence that phosphorylation of ABCA4 can regulate its function
Title: Posttranslational modifications of the photoreceptor-specific ABC transporter ABCA4.

results indicate that ATP binding cassette protein ABCA4 preferentially binds N-retinylidene-phosphatidylethanolamine with high affinity
Title: N-retinylidene-phosphatidylethanolamine is the preferred retinoid substrate for the photoreceptor-specific ABC transporter ABCA4 (ABCR).

The following ABCA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ABCA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa44182	NM_174221.2 Latest version!	Bos taurus ATP binding cassette subfamily A member 4 (ABCA4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa45172	XM_010803293.2 Latest version!	Bos taurus ATP binding cassette subfamily A member 4 (ABCA4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ABCA4 ( NM_174221.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776646.1 Bos taurus ATP binding cassette subfamily A member 4 (ABCA4), mRNA.

Price & Availability↑

CloneID	OBa44182
Clone ID Related Accession (Same CDS sequence)	NM_174221.2
Accession Version	NM_174221.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6846bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	retinal-specific ATP-binding cassette transporter
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U90126.1. On or before Mar 29, 2005 this sequence version replaced XM_600037.1, XM_616851.1, NM_174221.1. ##Evidence-Data-START## Transcript exon combination :: U90126.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGGGCTTTG CCAGACAGAT CAAGCTTCTG CTGTGGAAGA ACTGGACCCT GCGGAAAAGG 
CAAAAGATTC GCTTTGTGGT GGAACTCGTG TGGCCTTTAT CTTTATTTCT GGTCTTGATC 
TGGTTAAGGA ATGTCAACCC CCTCTACAGC AAACATGAAT GCCATTTTCC CAACAAGGCG 
ATGCCCTCAG CAGGAATGTT GCCATGGCTC CAGGGAATTT TCTGCAATGT GAACAATCCT 
TGTTTTCAAA GTCCCACTGC AGGAGAATCT CCTGGAATTG TGTCAAACTA TAACAACTCC 
ATCTTGGCAA GGGTGTATAG AGATTTTCAA GAACTCCTCA TGGATGCACC AGAGAGCCAG 
CACCTTGGTC AGGTTTGGAG AGAACTCCGC ACCTTGTCCC AGCTCATGAA CACCCTCCGA 
ATGCACCCCG AGAGGATTGC AGGAAGAGGA ATACGAATTC GGGAGGTCCT AAAAGATGAT 
GAAATGTTGA CACTGTTTCT CGTGAAGAAC ATTGGTCTGT CTGACTCAGT CGTCTACCTT 
CTGGTCAACT CCCAAGTCCG TCCAGAGCAG TTTGCTCGTG GAGTCCCAGA CCTGATGCTG 
AAGGACATCG CCTGCAGTGA GGCCCTCCTG GAGCGTTTCC TGATCTTTCC CCAGCGCCGT 
GCAGCCCAGA CAGTGCGTGG CAGCTTGTGC TCCCTCTCTC AGGGAACCCT ACAGTGGATG 
GAAGACACCC TGTACGCCAA CGTGGACTTC TTCAAGCTCT TCCATGTGTT TCCCAGACTC 
CTAGACAGCC GTTCTCAAGG TATGAATCTG AGATCCTGGG GAAGAATATT ATCTGATATG 
TCACCAAGAA TTCAAGAATT CATCCACCGG CCAAGTGTTC AAGACTTGCT GTGGGTGACC 
AGGCCACTCG TGCAGACTGG TGGCCCGGAG ACCTTCACGC AGCTGATGGG CATCCTGTCT 
GACCTCTTGT GTGGCTACCC CGAGGGAGGA GGATCTCGGG TGTTCTCCTT CAACTGGTAT 
GAAGACAATA ACTATAAGGC CTTTCTAGGG ATCGACTCCA CAAGGAAAGA TCCTATCTAT 
TCTTATGACG AAAGAACAAC AACCTTTTGT AACGCATTGA TCCAGAGCCT GGAGTCAAAC 
CCTCTAACCA AAATAGCCTG GAGGGCAGCA AAGCCTTTGT TGATGGGAAA AATCCTCTTT 
ACTCCAGATT CCCCTGCAAC ACGCAGGATA CTGAAGAATG CTAATTCGAC GTTTGAAGAG 
CTGGAGCGTG TCAGGAAGTT GGTCAAAGTC TGGGAAGAAG TAGGGCCCCA GATCTGGTAC 
TTCTTTGACA AGAGCACACA GATGAGCATG ATCAGAGACA CTCTGGAGAA CCCAACAGTA 
AAAGCTTTTT GGAATAGGCA GCTTGGTGAG GAAGGCATCA CTGCTGAAGC CGTCCTGAAC 
TTCCTCTATA ACGGGCCTCG AGAGGGCCAG GCTGACGATG TGGACAACTT CAACTGGAGG 
GATATATTTA ACATCACTGA TCGTGCCCTA CGCCTGGCTA ATCAATATCT GGAGTGCTTG 
ATCCTGGATA AGTTTGAAAG CTACGATGAT GAATTTCAAC TCACTCAGCG AGCCCTGTCC 
CTGCTGGAGG AAAACAGGTT CTGGGCTGGA GTGGTATTTC CGGACATGCA CCCCTGGACC 
AGTTCCTTAC CACCCCATGT GAAGTACAAG ATCCGAATGG ACATAGATGT GGTGGAGAAA 
ACCAATAAGA TCAAAGACAG GTACTGGGAT TCTGGCCCCA GAGCTGATCC TGTGGAAGAC 
TTCCGATACA TCTGGGGAGG GTTTGCCTAT CTGCAGGACA TGGTTGAACA TGGGATCACG 
AGGAGTCAGG CCCAGGAGGA GGTTCCGGTT GGAATCTATC TCCAGCAGAT GCCTTACCCC 
TGCTTCGTGG ATGACTCTTT TATGATCATC CTGAACCGCT GTTTCCCTAT CTTCATGGTG 
CTGGCCTGGA TCTACTCTGT CTCCATGACT GTAAAGAGCA TTGTCTTGGA GAAGGAGTTG 
AGACTGAAGG AAACCTTGAA AAATCAGGGT GTCTCCAACA GGGTGATTTG GTGTACCTGG 
TTCCTGGACA GCTTCTCTAT CATGTCGATG AGCATCTGCC TGCTGACAAT ATTCATCATG 
CATGGAAGGA TCCTGCATTA CAGCAATCCG TTCATCCTCT TCCTGTTCCT GTTGGCTTTC 
TCCATTGCTA CGATCATGCA GTGCTTCCTG CTCAGCACCT TCTTCTCCAG GGCCAGTCTG 
GCAGCAGCCT GCAGTGGTGT CATCTACTTC ACCCTCTACC TGCCACACAT TCTCTGCTTC 
GCTTGGCAGG ACCGAATTAC AGCCGACATG AAGATGGCTG TGAGCCTGCT GTCTCCTGTG 
GCATTTGGGT TTGGCACTGA GTACCTGGCT CRTTTTGAAG AGCAAGGTGT GGGGCTGCAG 
TGGAGCAATA TTGGGAACAG TCCCATGGAA GGGGATGAAT TCAGTTTTCT GATGTCCATG 
AAGATGATGC TCCTTGATGC TGCTCTGTAT GGCTTGCTTG CCTGGTATCT TGACCAGGTG 
TTCCCAGGGG ACTATGGAAC CCCACTTCCT TGGTACTTCC TTCTACAAGA GTCTTACTGG 
CTAGGTGGTG AAGGCTGTTC AACCAGAGAA GAAAGAGCCT TGGAAAAGAC AGAACCAATA 
ACAGAGGAAA TGGAGGACCC AGAATACCCA GAAGGAATAA ATGACTGCTT CTTTGAACGT 
GAGCTTCCAG GCTTGGTTCC CGGAGTATGT GTGAAGAATC TGGTAAAGAT ATTTGAGCCC 
TATGGCAGGC CAGCCGTGGA CCGTCTTAAC ATTACCTTCT ATGAAAGTCA GATTACTGCA 
TTCCTCGGTC ACAATGGAGC AGGGAAAACC ACGACTTTGT CCATCATGAC CGGTCTGTTG 
CCACCGACAT CCGGGACTGT GCTTGTTGGG GGAAAGGACA TTGAAACCAA CCTGGATGCT 
ATCCGGCAGA GCCTTGGCAT GTGTCCACAG CACAACATCC TGTTCCACCA CCTCACCGTG 
GCTGAACACA TCCTGTTCTA CGCGCAGCTG AAAGGGAGGT CCTGGGACGA GGCCCAGCTG 
GAGATGGAAG CAATGCTGGA GGACACAGGC CTCCACCACA AGAGGAACGA AGAGGCTAGG 
GATCTGTCAG GTGGCGTGCA GAGGAAGCTG TCTGTGGCCA TTGCCTTCGT GGGAGATGCC 
AAGGTGGTGG TTCTGGATGA GCCCACCTCT GGGGTGGACC CCTACTCACG TCGCTCAATC 
TGGGACCTGC TCCTGAAGTA TCGCTCAGGC AGAACCATCA TCATGTCCAC TCACCACATG 
GACGAGGCCG ACATCCTCGG GGACCGCATC GCCATCATAT CCCAGGGACG GCTCTACTGC 
TCTGGAACCC CACTGTTCCT GAAGAACTGC TTTGGCACAG GCTTCTACTT AACCTTGGTG 
CGCAGGATGA AGACTATTCA GAGCCAAGGA AGAGGCCGCG AGGCGACCTG CAGCTGTGCG 
TCTAAGGGCT TCTCCGTCAG GTGTCCGGCC TGTGCCGAGG CGATAACTCC AGAACAAGTC 
TTGGACGGGG ATGTGAATGA ACTGACGGAT ATGGTTCACC ACCATGTTCC AGAGGCGAAG 
CTGGTAGAAT GCATTGGTCA AGAACTTATC TTCCTTCTTC CAAATAAGAA TTTCAAGCAG 
AGAGCATATG CTAGCCTTTT CAGAGAGCTG GAGGAGACGC TGGCTGACCT GGGACTCAGC 
AGTTTTGGAA TTTCTGACAC TCCCCTGGAA GAGATTTTCC TGAAGGTCAC AGAGGATTTG 
GATTCAGGAC ATCTGTTTGC TGGAGGCACT CAGCAGAAAA GAGAAAACAT CAACCTTCGA 
CACCCCTGTT CGGGTCCTAG CGAGAAGGCC GGACAGACTC CCCAGGGCTC CAGCAGCCAC 
CCCGGAGAGC CAGCTGCTCA CCCGGAGGGC CAGCCTCCAC CGGAGCGGGA GGGACACAGC 
CGACTGAACT CAGGAGCTCG GCTTATTGTC CAGCACGTGC AGGCACTGCT GGTCAAGAGG 
TTCCAGCACA CCATCCGCAG CCACAAGGAC TTCCTGGCCC AGATTGTGCT CCCGGCCACT 
TTTGTGTTTT TGGCTCTAAT GCTTTCCCTC ATCATCCCTC CTTTTGGTGA ATATCCTGCT 
TTGACCCTTC ACCCCTGGAT GTATGGACAG CAGTACACCT TCTTCAGCAT GGACCAGCCG 
GACAGTGAAT GGCTCTCAGC ACTTGCAGAT GTCCTTGTGA ATAAGCCAGG CTTTGGCAAC 
CGCTGCCTGA AGGAAGAGTG GCTTCCGGAG TTCCCCTGTG GAAATTCAAG CCCCTGGAAG 
ACTCCATCTG TGTCCCCGGA TGTCACCCAC TTGCTCCAAC AGCAGAAATG GACAGCGGAC 
CAACCTTCAC CTTCCTGCAG GTGCAGCACC AGAGAGAAGC TCACCATGCT TCCTGAGTGC 
CCTGAGGGCG CAGGGGGCCT CCCACCCCCG CAGAGAATAC AACGCAGCAC TGAAATTCTG 
CAGGACCTTA CAGACAGGAA TGTCTCCGAC TTCCTGGTAA AAACATATCC TGCTCTTATC 
CGAAGCAGTT TGAAGAGCAA ATTCTGGGTC AATGAACAGA GGTATGGAGG AATTTCTGTC 
GGAGGAAAGC TCCCAGCTCC CCCCTTCACT GGAGAAGCAC TTGTTGGATT TTTAAGTGAC 
CTTGGCCAGT TAATGAATGT GAGCGGGGGC CCTATGACCA GAGAGGCTGC TAAAGAAATG 
CCTGCTTTCC TTAAACAACT AGAAACTGAA GACAATATTA AGGTGTGGTT TAACAACAAA 
GGTTGGCATG CTCTGGTCAG CTTCCTCAAC GTGGCCCACA ACGCCATCTT ACGGGCTAGC 
CTGCACAAGG ACAAGAACCC CGAGGAGTAT GGCATCACCG TCATAAGCCA GCCCTTGAAC 
CTGACCAAGG AGCAGCTCTC TGAGATCACA GTACTGACCA CTTCGGTGGA CGCTGTAGTG 
GCCATCTGCG TGATTTTTGC CATGTCCTTC GTCCCTGCCA GCTTTGTCCT TTATTTGATC 
CAGGAGAGGG TGAACAAGGC CAAGCACCTC CAGTTTGTCA GCGGAGTGAG CCCCACCACC 
TACTGGCTGA CCAACTTCCT CTGGGACATC ATGAATTACA CTGTAAGTGC TGCACTGGTG 
GTGGGCATCT TCATCGGGTT TCAGAAGAAA GCCTACACTT CTTCAGAGAA CCTTCCAGCC 
CTTGTTGCGC TGCTCATGCT GTATGGGTGG GCAGTCATTC CCATGATGTA TCCGGCATCC 
TTCCTGTTCG ATATCCCCAG CACTGCCTAT GTGGCCTTAT CTTGTGCTAA TCTGTTCATT 
GGCATCAACA GCAGTGCCAT CACTTTCGTC CTGGAATTAT TTGAGAATAA CCGGACGCTG 
CTCAGGATCA ATGCCATGCT GAGGAAGCTG CTCATTATCT TCCCCCACTT CTGCCTGGGC 
CGGGGTCTCA TTGACCTGGC ACTGAGCCAG GCAGTGACAG ACGTCTATGC CCAGTTTGGT 
GAGGCGCACT CTTCAAATCC ATTCCAGTGG GACCTGATTG GGAAAAACCT GGCTGCCATG 
GCAGTAGAAG GTGTGGTGTA CTTCCTCTTG ACGCTCCTCA TCCAATACCA GTTCTTCTTC 
AGCCGATGGA CTACCGAGCC TGCAAAGGAG CCCATCACAG ATGAAGATGA TGATGTGGCT 
GAAGAAAGGC AGAGAATTAT TAGTGGAGGA AATAAAACTG ATATCTTAAG GCTAAATGAA 
CTCACCAAGG TTTATTCAGG CACCTCCAGC CCAGCAGTGG ACAGGCTGTG TGTGGGAGTC 
CGCCCTGGAG AGTGCTTTGG TCTCCTGGGT GTGAACGGAG CTGGCAAAAC CACCACATTC 
AAGATGCTCA CTGGGGACAC CGCAGTGACC TCAGGCGATG CCACTGTCGC AGGCAAGAGT 
ATTTTGACCA ATATTTCTGA CGTCCATCAA AGTATGGGCT ACTGTCCTCA GTTTGATGCA 
ATCGATGACC TGCTTACTGG GCGAGAACAT CTTTACCTTT ATGCCCGGCT TCGAGGTGTA 
CCAGCAGAGG AAATTGAGAG GGTGACAAAC TGGAGTATTC AGAGCCTGGG CCTGTCTCTC 
TATGCAGACC GCCTGGCCGG CACCTACAGT GGAGGAAATA AGCGGAAACT CTCCACAGCC 
ATTGCGCTCA TAGGCTGCCC CCCACTGGTG CTGCTGGATG AGCCCACCAC AGGCATGGAC 
CCCCAGGCAC GCCGCATGCT GTGGAACACT ATCATGGGCA TCATCAGAGA AGAGAGAGCC 
GTGGTCCTCA CTTCCCACAG CATGGAAGAG TGCGAGGCCC TGTGTACCCG GCTGGCCATC 
ATGGTGAAGG GCGCCTTTCA GTGTCTGGGC ACCATCCAGC ACCTCAAGTC CAAGTTTGGA 
GACGGCTACA TTGTCACGAT GAAAATCAGG TCCCCAAAGG ACGACTTGCT TCCTGACCTG 
GGTCCGGTGG AGCAGTTCTT CCAGGGCAAC TTCCCAGGCA GCGTCCAGAG GGAGCGGCAC 
TACAACACGC TGCAGTTCCA GGTCTCCTCC TCCTCCCTGG CCAGGATCTT CCGGCTGCTC 
GTCTCCCACA AGGACAGCCT GCTCATCGAG GAGTACTCAG TCACGCAGAC CACACTGGAC 
CAGGTATTTG TGAACTTTGC TAAACAGCAG AATGAAACTT ACGACCTCCC TTTGCACCCT 
CGAACTGCAG GAGCCAGCCG ACAGGCCAAG GAAGTGGACA AAGGGAATTC TGCACCTCAA 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776646.1
CDS	101..6946
Translation	MGFARQIKLLLWKNWTLRKRQKIRFVVELVWPLSLFLVLIWLRNVNPLYSKHECHFPNKAMPSAGMLPWLQGIFCNVNNPCFQSPTAGESPGIVSNYNNSILARVYRDFQELLMDAPESQHLGQVWRELRTLSQLMNTLRMHPERIAGRGIRIREVLKDDEMLTLFLVKNIGLSDSVVYLLVNSQVRPEQFARGVPDLMLKDIACSEALLERFLIFPQRRAAQTVRGSLCSLSQGTLQWMEDTLYANVDFFKLFHVFPRLLDSRSQGMNLRSWGRILSDMSPRIQEFIHRPSVQDLLWVTRPLVQTGGPETFTQLMGILSDLLCGYPEGGGSRVFSFNWYEDNNYKAFLGIDSTRKDPIYSYDERTTTFCNALIQSLESNPLTKIAWRAAKPLLMGKILFTPDSPATRRILKNANSTFEELERVRKLVKVWEEVGPQIWYFFDKSTQMSMIRDTLENPTVKAFWNRQLGEEGITAEAVLNFLYNGPREGQADDVDNFNWRDIFNITDRALRLANQYLECLILDKFESYDDEFQLTQRALSLLEENRFWAGVVFPDMHPWTSSLPPHVKYKIRMDIDVVEKTNKIKDRYWDSGPRADPVEDFRYIWGGFAYLQDMVEHGITRSQAQEEVPVGIYLQQMPYPCFVDDSFMIILNRCFPIFMVLAWIYSVSMTVKSIVLEKELRLKETLKNQGVSNRVIWCTWFLDSFSIMSMSICLLTIFIMHGRILHYSNPFILFLFLLAFSIATIMQCFLLSTFFSRASLAAACSGVIYFTLYLPHILCFAWQDRITADMKMAVSLLSPVAFGFGTEYLAnullFEEQGVGLQWSNIGNSPMEGDEFSFLMSMKMMLLDAALYGLLAWYLDQVFPGDYGTPLPWYFLLQESYWLGGEGCSTREERALEKTEPITEEMEDPEYPEGINDCFFERELPGLVPGVCVKNLVKIFEPYGRPAVDRLNITFYESQITAFLGHNGAGKTTTLSIMTGLLPPTSGTVLVGGKDIETNLDAIRQSLGMCPQHNILFHHLTVAEHILFYAQLKGRSWDEAQLEMEAMLEDTGLHHKRNEEARDLSGGVQRKLSVAIAFVGDAKVVVLDEPTSGVDPYSRRSIWDLLLKYRSGRTIIMSTHHMDEADILGDRIAIISQGRLYCSGTPLFLKNCFGTGFYLTLVRRMKTIQSQGRGREATCSCASKGFSVRCPACAEAITPEQVLDGDVNELTDMVHHHVPEAKLVECIGQELIFLLPNKNFKQRAYASLFRELEETLADLGLSSFGISDTPLEEIFLKVTEDLDSGHLFAGGTQQKRENINLRHPCSGPSEKAGQTPQGSSSHPGEPAAHPEGQPPPEREGHSRLNSGARLIVQHVQALLVKRFQHTIRSHKDFLAQIVLPATFVFLALMLSLIIPPFGEYPALTLHPWMYGQQYTFFSMDQPDSEWLSALADVLVNKPGFGNRCLKEEWLPEFPCGNSSPWKTPSVSPDVTHLLQQQKWTADQPSPSCRCSTREKLTMLPECPEGAGGLPPPQRIQRSTEILQDLTDRNVSDFLVKTYPALIRSSLKSKFWVNEQRYGGISVGGKLPAPPFTGEALVGFLSDLGQLMNVSGGPMTREAAKEMPAFLKQLETEDNIKVWFNNKGWHALVSFLNVAHNAILRASLHKDKNPEEYGITVISQPLNLTKEQLSEITVLTTSVDAVVAICVIFAMSFVPASFVLYLIQERVNKAKHLQFVSGVSPTTYWLTNFLWDIMNYTVSAALVVGIFIGFQKKAYTSSENLPALVALLMLYGWAVIPMMYPASFLFDIPSTAYVALSCANLFIGINSSAITFVLELFENNRTLLRINAMLRKLLIIFPHFCLGRGLIDLALSQAVTDVYAQFGEAHSSNPFQWDLIGKNLAAMAVEGVVYFLLTLLIQYQFFFSRWTTEPAKEPITDEDDDVAEERQRIISGGNKTDILRLNELTKVYSGTSSPAVDRLCVGVRPGECFGLLGVNGAGKTTTFKMLTGDTAVTSGDATVAGKSILTNISDVHQSMGYCPQFDAIDDLLTGREHLYLYARLRGVPAEEIERVTNWSIQSLGLSLYADRLAGTYSGGNKRKLSTAIALIGCPPLVLLDEPTTGMDPQARRMLWNTIMGIIREERAVVLTSHSMEECEALCTRLAIMVKGAFQCLGTIQHLKSKFGDGYIVTMKIRSPKDDLLPDLGPVEQFFQGNFPGSVQRERHYNTLQFQVSSSSLARIFRLLVSHKDSLLIEEYSVTQTTLDQVFVNFAKQQNETYDLPLHPRTAGASRQAKEVDKGNSAPQG

Target ORF information:

RefSeq Version	NM_174221.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP binding cassette subfamily A member 4 (ABCA4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174221.2

ORF Insert Sequence:

ATGGGCTTTG CCAGACAGAT CAAGCTTCTG CTGTGGAAGA ACTGGACCCT GCGGAAAAGG 
CAAAAGATTC GCTTTGTGGT GGAACTCGTG TGGCCTTTAT CTTTATTTCT GGTCTTGATC 
TGGTTAAGGA ATGTCAACCC CCTCTACAGC AAACATGAAT GCCATTTTCC CAACAAGGCG 
ATGCCCTCAG CAGGAATGTT GCCATGGCTC CAGGGAATTT TCTGCAATGT GAACAATCCT 
TGTTTTCAAA GTCCCACTGC AGGAGAATCT CCTGGAATTG TGTCAAACTA TAACAACTCC 
ATCTTGGCAA GGGTGTATAG AGATTTTCAA GAACTCCTCA TGGATGCACC AGAGAGCCAG 
CACCTTGGTC AGGTTTGGAG AGAACTCCGC ACCTTGTCCC AGCTCATGAA CACCCTCCGA 
ATGCACCCCG AGAGGATTGC AGGAAGAGGA ATACGAATTC GGGAGGTCCT AAAAGATGAT 
GAAATGTTGA CACTGTTTCT CGTGAAGAAC ATTGGTCTGT CTGACTCAGT CGTCTACCTT 
CTGGTCAACT CCCAAGTCCG TCCAGAGCAG TTTGCTCGTG GAGTCCCAGA CCTGATGCTG 
AAGGACATCG CCTGCAGTGA GGCCCTCCTG GAGCGTTTCC TGATCTTTCC CCAGCGCCGT 
GCAGCCCAGA CAGTGCGTGG CAGCTTGTGC TCCCTCTCTC AGGGAACCCT ACAGTGGATG 
GAAGACACCC TGTACGCCAA CGTGGACTTC TTCAAGCTCT TCCATGTGTT TCCCAGACTC 
CTAGACAGCC GTTCTCAAGG TATGAATCTG AGATCCTGGG GAAGAATATT ATCTGATATG 
TCACCAAGAA TTCAAGAATT CATCCACCGG CCAAGTGTTC AAGACTTGCT GTGGGTGACC 
AGGCCACTCG TGCAGACTGG TGGCCCGGAG ACCTTCACGC AGCTGATGGG CATCCTGTCT 
GACCTCTTGT GTGGCTACCC CGAGGGAGGA GGATCTCGGG TGTTCTCCTT CAACTGGTAT 
GAAGACAATA ACTATAAGGC CTTTCTAGGG ATCGACTCCA CAAGGAAAGA TCCTATCTAT 
TCTTATGACG AAAGAACAAC AACCTTTTGT AACGCATTGA TCCAGAGCCT GGAGTCAAAC 
CCTCTAACCA AAATAGCCTG GAGGGCAGCA AAGCCTTTGT TGATGGGAAA AATCCTCTTT 
ACTCCAGATT CCCCTGCAAC ACGCAGGATA CTGAAGAATG CTAATTCGAC GTTTGAAGAG 
CTGGAGCGTG TCAGGAAGTT GGTCAAAGTC TGGGAAGAAG TAGGGCCCCA GATCTGGTAC 
TTCTTTGACA AGAGCACACA GATGAGCATG ATCAGAGACA CTCTGGAGAA CCCAACAGTA 
AAAGCTTTTT GGAATAGGCA GCTTGGTGAG GAAGGCATCA CTGCTGAAGC CGTCCTGAAC 
TTCCTCTATA ACGGGCCTCG AGAGGGCCAG GCTGACGATG TGGACAACTT CAACTGGAGG 
GATATATTTA ACATCACTGA TCGTGCCCTA CGCCTGGCTA ATCAATATCT GGAGTGCTTG 
ATCCTGGATA AGTTTGAAAG CTACGATGAT GAATTTCAAC TCACTCAGCG AGCCCTGTCC 
CTGCTGGAGG AAAACAGGTT CTGGGCTGGA GTGGTATTTC CGGACATGCA CCCCTGGACC 
AGTTCCTTAC CACCCCATGT GAAGTACAAG ATCCGAATGG ACATAGATGT GGTGGAGAAA 
ACCAATAAGA TCAAAGACAG GTACTGGGAT TCTGGCCCCA GAGCTGATCC TGTGGAAGAC 
TTCCGATACA TCTGGGGAGG GTTTGCCTAT CTGCAGGACA TGGTTGAACA TGGGATCACG 
AGGAGTCAGG CCCAGGAGGA GGTTCCGGTT GGAATCTATC TCCAGCAGAT GCCTTACCCC 
TGCTTCGTGG ATGACTCTTT TATGATCATC CTGAACCGCT GTTTCCCTAT CTTCATGGTG 
CTGGCCTGGA TCTACTCTGT CTCCATGACT GTAAAGAGCA TTGTCTTGGA GAAGGAGTTG 
AGACTGAAGG AAACCTTGAA AAATCAGGGT GTCTCCAACA GGGTGATTTG GTGTACCTGG 
TTCCTGGACA GCTTCTCTAT CATGTCGATG AGCATCTGCC TGCTGACAAT ATTCATCATG 
CATGGAAGGA TCCTGCATTA CAGCAATCCG TTCATCCTCT TCCTGTTCCT GTTGGCTTTC 
TCCATTGCTA CGATCATGCA GTGCTTCCTG CTCAGCACCT TCTTCTCCAG GGCCAGTCTG 
GCAGCAGCCT GCAGTGGTGT CATCTACTTC ACCCTCTACC TGCCACACAT TCTCTGCTTC 
GCTTGGCAGG ACCGAATTAC AGCCGACATG AAGATGGCTG TGAGCCTGCT GTCTCCTGTG 
GCATTTGGGT TTGGCACTGA GTACCTGGCT CRTTTTGAAG AGCAAGGTGT GGGGCTGCAG 
TGGAGCAATA TTGGGAACAG TCCCATGGAA GGGGATGAAT TCAGTTTTCT GATGTCCATG 
AAGATGATGC TCCTTGATGC TGCTCTGTAT GGCTTGCTTG CCTGGTATCT TGACCAGGTG 
TTCCCAGGGG ACTATGGAAC CCCACTTCCT TGGTACTTCC TTCTACAAGA GTCTTACTGG 
CTAGGTGGTG AAGGCTGTTC AACCAGAGAA GAAAGAGCCT TGGAAAAGAC AGAACCAATA 
ACAGAGGAAA TGGAGGACCC AGAATACCCA GAAGGAATAA ATGACTGCTT CTTTGAACGT 
GAGCTTCCAG GCTTGGTTCC CGGAGTATGT GTGAAGAATC TGGTAAAGAT ATTTGAGCCC 
TATGGCAGGC CAGCCGTGGA CCGTCTTAAC ATTACCTTCT ATGAAAGTCA GATTACTGCA 
TTCCTCGGTC ACAATGGAGC AGGGAAAACC ACGACTTTGT CCATCATGAC CGGTCTGTTG 
CCACCGACAT CCGGGACTGT GCTTGTTGGG GGAAAGGACA TTGAAACCAA CCTGGATGCT 
ATCCGGCAGA GCCTTGGCAT GTGTCCACAG CACAACATCC TGTTCCACCA CCTCACCGTG 
GCTGAACACA TCCTGTTCTA CGCGCAGCTG AAAGGGAGGT CCTGGGACGA GGCCCAGCTG 
GAGATGGAAG CAATGCTGGA GGACACAGGC CTCCACCACA AGAGGAACGA AGAGGCTAGG 
GATCTGTCAG GTGGCGTGCA GAGGAAGCTG TCTGTGGCCA TTGCCTTCGT GGGAGATGCC 
AAGGTGGTGG TTCTGGATGA GCCCACCTCT GGGGTGGACC CCTACTCACG TCGCTCAATC 
TGGGACCTGC TCCTGAAGTA TCGCTCAGGC AGAACCATCA TCATGTCCAC TCACCACATG 
GACGAGGCCG ACATCCTCGG GGACCGCATC GCCATCATAT CCCAGGGACG GCTCTACTGC 
TCTGGAACCC CACTGTTCCT GAAGAACTGC TTTGGCACAG GCTTCTACTT AACCTTGGTG 
CGCAGGATGA AGACTATTCA GAGCCAAGGA AGAGGCCGCG AGGCGACCTG CAGCTGTGCG 
TCTAAGGGCT TCTCCGTCAG GTGTCCGGCC TGTGCCGAGG CGATAACTCC AGAACAAGTC 
TTGGACGGGG ATGTGAATGA ACTGACGGAT ATGGTTCACC ACCATGTTCC AGAGGCGAAG 
CTGGTAGAAT GCATTGGTCA AGAACTTATC TTCCTTCTTC CAAATAAGAA TTTCAAGCAG 
AGAGCATATG CTAGCCTTTT CAGAGAGCTG GAGGAGACGC TGGCTGACCT GGGACTCAGC 
AGTTTTGGAA TTTCTGACAC TCCCCTGGAA GAGATTTTCC TGAAGGTCAC AGAGGATTTG 
GATTCAGGAC ATCTGTTTGC TGGAGGCACT CAGCAGAAAA GAGAAAACAT CAACCTTCGA 
CACCCCTGTT CGGGTCCTAG CGAGAAGGCC GGACAGACTC CCCAGGGCTC CAGCAGCCAC 
CCCGGAGAGC CAGCTGCTCA CCCGGAGGGC CAGCCTCCAC CGGAGCGGGA GGGACACAGC 
CGACTGAACT CAGGAGCTCG GCTTATTGTC CAGCACGTGC AGGCACTGCT GGTCAAGAGG 
TTCCAGCACA CCATCCGCAG CCACAAGGAC TTCCTGGCCC AGATTGTGCT CCCGGCCACT 
TTTGTGTTTT TGGCTCTAAT GCTTTCCCTC ATCATCCCTC CTTTTGGTGA ATATCCTGCT 
TTGACCCTTC ACCCCTGGAT GTATGGACAG CAGTACACCT TCTTCAGCAT GGACCAGCCG 
GACAGTGAAT GGCTCTCAGC ACTTGCAGAT GTCCTTGTGA ATAAGCCAGG CTTTGGCAAC 
CGCTGCCTGA AGGAAGAGTG GCTTCCGGAG TTCCCCTGTG GAAATTCAAG CCCCTGGAAG 
ACTCCATCTG TGTCCCCGGA TGTCACCCAC TTGCTCCAAC AGCAGAAATG GACAGCGGAC 
CAACCTTCAC CTTCCTGCAG GTGCAGCACC AGAGAGAAGC TCACCATGCT TCCTGAGTGC 
CCTGAGGGCG CAGGGGGCCT CCCACCCCCG CAGAGAATAC AACGCAGCAC TGAAATTCTG 
CAGGACCTTA CAGACAGGAA TGTCTCCGAC TTCCTGGTAA AAACATATCC TGCTCTTATC 
CGAAGCAGTT TGAAGAGCAA ATTCTGGGTC AATGAACAGA GGTATGGAGG AATTTCTGTC 
GGAGGAAAGC TCCCAGCTCC CCCCTTCACT GGAGAAGCAC TTGTTGGATT TTTAAGTGAC 
CTTGGCCAGT TAATGAATGT GAGCGGGGGC CCTATGACCA GAGAGGCTGC TAAAGAAATG 
CCTGCTTTCC TTAAACAACT AGAAACTGAA GACAATATTA AGGTGTGGTT TAACAACAAA 
GGTTGGCATG CTCTGGTCAG CTTCCTCAAC GTGGCCCACA ACGCCATCTT ACGGGCTAGC 
CTGCACAAGG ACAAGAACCC CGAGGAGTAT GGCATCACCG TCATAAGCCA GCCCTTGAAC 
CTGACCAAGG AGCAGCTCTC TGAGATCACA GTACTGACCA CTTCGGTGGA CGCTGTAGTG 
GCCATCTGCG TGATTTTTGC CATGTCCTTC GTCCCTGCCA GCTTTGTCCT TTATTTGATC 
CAGGAGAGGG TGAACAAGGC CAAGCACCTC CAGTTTGTCA GCGGAGTGAG CCCCACCACC 
TACTGGCTGA CCAACTTCCT CTGGGACATC ATGAATTACA CTGTAAGTGC TGCACTGGTG 
GTGGGCATCT TCATCGGGTT TCAGAAGAAA GCCTACACTT CTTCAGAGAA CCTTCCAGCC 
CTTGTTGCGC TGCTCATGCT GTATGGGTGG GCAGTCATTC CCATGATGTA TCCGGCATCC 
TTCCTGTTCG ATATCCCCAG CACTGCCTAT GTGGCCTTAT CTTGTGCTAA TCTGTTCATT 
GGCATCAACA GCAGTGCCAT CACTTTCGTC CTGGAATTAT TTGAGAATAA CCGGACGCTG 
CTCAGGATCA ATGCCATGCT GAGGAAGCTG CTCATTATCT TCCCCCACTT CTGCCTGGGC 
CGGGGTCTCA TTGACCTGGC ACTGAGCCAG GCAGTGACAG ACGTCTATGC CCAGTTTGGT 
GAGGCGCACT CTTCAAATCC ATTCCAGTGG GACCTGATTG GGAAAAACCT GGCTGCCATG 
GCAGTAGAAG GTGTGGTGTA CTTCCTCTTG ACGCTCCTCA TCCAATACCA GTTCTTCTTC 
AGCCGATGGA CTACCGAGCC TGCAAAGGAG CCCATCACAG ATGAAGATGA TGATGTGGCT 
GAAGAAAGGC AGAGAATTAT TAGTGGAGGA AATAAAACTG ATATCTTAAG GCTAAATGAA 
CTCACCAAGG TTTATTCAGG CACCTCCAGC CCAGCAGTGG ACAGGCTGTG TGTGGGAGTC 
CGCCCTGGAG AGTGCTTTGG TCTCCTGGGT GTGAACGGAG CTGGCAAAAC CACCACATTC 
AAGATGCTCA CTGGGGACAC CGCAGTGACC TCAGGCGATG CCACTGTCGC AGGCAAGAGT 
ATTTTGACCA ATATTTCTGA CGTCCATCAA AGTATGGGCT ACTGTCCTCA GTTTGATGCA 
ATCGATGACC TGCTTACTGG GCGAGAACAT CTTTACCTTT ATGCCCGGCT TCGAGGTGTA 
CCAGCAGAGG AAATTGAGAG GGTGACAAAC TGGAGTATTC AGAGCCTGGG CCTGTCTCTC 
TATGCAGACC GCCTGGCCGG CACCTACAGT GGAGGAAATA AGCGGAAACT CTCCACAGCC 
ATTGCGCTCA TAGGCTGCCC CCCACTGGTG CTGCTGGATG AGCCCACCAC AGGCATGGAC 
CCCCAGGCAC GCCGCATGCT GTGGAACACT ATCATGGGCA TCATCAGAGA AGAGAGAGCC 
GTGGTCCTCA CTTCCCACAG CATGGAAGAG TGCGAGGCCC TGTGTACCCG GCTGGCCATC 
ATGGTGAAGG GCGCCTTTCA GTGTCTGGGC ACCATCCAGC ACCTCAAGTC CAAGTTTGGA 
GACGGCTACA TTGTCACGAT GAAAATCAGG TCCCCAAAGG ACGACTTGCT TCCTGACCTG 
GGTCCGGTGG AGCAGTTCTT CCAGGGCAAC TTCCCAGGCA GCGTCCAGAG GGAGCGGCAC 
TACAACACGC TGCAGTTCCA GGTCTCCTCC TCCTCCCTGG CCAGGATCTT CCGGCTGCTC 
GTCTCCCACA AGGACAGCCT GCTCATCGAG GAGTACTCAG TCACGCAGAC CACACTGGAC 
CAGGTATTTG TGAACTTTGC TAAACAGCAG AATGAAACTT ACGACCTCCC TTTGCACCCT 
CGAACTGCAG GAGCCAGCCG ACAGGCCAAG GAAGTGGACA AAGGGAATTC TGCACCTCAA 
GGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ABCA4 ( XM_010803293.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010801595.1 Bos taurus ATP binding cassette subfamily A member 4 (ABCA4), transcript variant X2, mRNA.

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CloneID	OBa45172
Clone ID Related Accession (Same CDS sequence)	XM_010803293.2
Accession Version	XM_010803293.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6147bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	retinal-specific ATP-binding cassette transporter isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103869.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010803293.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTTTG CCAGACAGAT CAAGCTTCTG CTGTGGAAGA ACTGGACCCT GCGGAAAAGG 
CAAAAGATTC GCTTTGTGGT GGAACTCGTG TGGCCTTTAT CTTTATTTCT GGTCTTGATC 
TGGTTAAGGA ATGTCAACCC CCTCTACAGC AAACATGAAT GCCATTTTCC CAACAAGGCG 
ATGCCCTCAG CAGGAATGTT GCCATGGCTC CAGGGAATTT TCTGCAATGT GAACAATCCT 
TGTTTTCAAA GTCCCACTGC AGGAGAATCT CCTGGAATTG TGTCAAACTA TAACAACTCC 
ATCTTGGCAA GGGTGTATAG AGATTTTCAA GAACTCCTCA TGGATGCACC AGAGAGCCAG 
CACCTTGGTC AGGTTTGGAG AGAACTCCGC ACCTTGTCCC AGCTCATGAA CACCCTCCGA 
ATGCACCCCG AGAGGATTGC AGGAAGAGGA ATACGAATTC GGGAGGTCCT AAAAGATGAT 
GAAATGTTGA CACTGTTTCT CGTGAAGAAC ATTGGTCTGT CTGACTCAGT CGTCTACCTT 
CTGGTCAACT CCCAAGTCCG TCCAGAGCAG TTTGCTCGTG GAGTCCCAGA CCTGATGCTG 
AAGGACATCG CCTGCAGTGA GGCCCTCCTG GAGCGTTTCC TGATCTTTCC CCAGCGCCGT 
GCAGCCCAGA CAGTGCGTGG CAGCTTGTGC TCCCTCTCTC AGGGAACCCT ACAGTGGATG 
GAAGACACCC TGTACGCCAA CGTGGACTTC TTCAAGCTCT TCCATGTGTT TCCCAGACTC 
CTAGACAGCC GTTCTCAAGG TATGAATCTG AGATCCTGGG GAAGAATATT ATCTGATATG 
TCACCAAGAA TTCAAGAATT CATCCACCGG CCAAGTGTTC AAGACTTGCT GTGGGTGACC 
AGGCCACTCG TGCAGACTGG TGGCCCGGAG ACCTTCACGC AGCTGATGGG CATCCTGTCT 
GACCTCTTGT GTGGCTACCC CGAGGGAGGA GGATCTCGGG TGTTCTCCTT CAACTGGTAT 
GAAGACAATA ACTATAAGGC CTTTCTAGGG ATCGACTCCA CAAGGAAAGA TCCTATCTAT 
TCTTATGATG AAAGAACAAC AACCTTTTGT AACGCATTGA TCCAGAGCCT GGAGTCAAAC 
CCTCTAACCA AAATAGCCTG GAGGGCAGCA AAGCCTTTGT TGATGGGAAA AATCCTCTTT 
ACTCCAGATT CCCCTGCAAC ACGCAGGATA CTGAAGAATG CTAATTCGAC GTTTGAAGAG 
CTGGAGCGTG TCAGGAAGTT GGTCAAAGTC TGGGAAGAAG TAGGGCCCCA GATCTGGTAC 
TTCTTTGACA AGAGCACACA GATGAGCATG ATCAGAGACA CTCTGGAGAA CCCAACAGTA 
AAAGCTTTTT GGAATAGGCA GCTTGGTGAG GAAGGCATCA CTGCTGAAGC CGTCCTGAAC 
TTCCTCTATA ACGGGCCTCG AGAGGGCCAG GCTGACGATG TGGACAACTT CAACTGGAGG 
GATATATTTA ACATCACTGA TCGTGCCCTA CGCCTGGCTA ATCAATATCT GGAGTGCTTG 
ATCCTGGATA AGTTTGAAAG CTACGATGAT GAATTTCAAC TCACTCAGCG AGCCCTGTCC 
CTGCTGGAGG AAAACAGGTT CTGGGCTGGA GTGGTATTTC CGGACATGCA CCCCTGGACC 
AGTTCCTTAC CACCCCATGT GAAGTACAAG ATCCGAATGG ACATAGATGT GGTGGAGAAA 
ACCAATAAGA TCAAAGACAG GTACTGGGAT TCTGGCCCCA GAGCTGATCC TGTGGAAGAC 
TTCCGATACA TCTGGGGAGG GTTTGCCTAT CTGCAGGACA TGGTTGAACA TGGGATCACG 
AGGAGTCAGG CCCAGGAGGA GGTTCCGGTT GGAATCTATC TCCAGCAGAT GCCTTACCCC 
TGCTTCGTGG ATGACTCTTT TATGATCATC CTGAACCGCT GTTTCCCTAT CTTCATGGTG 
CTGGCCTGGA TCTACTCTGT CTCCATGACT GTAAAGAGCA TTGTCTTGGA GAAGGAGTTG 
AGACTGAAGG AAACCTTGAA AAATCAGGGT GTCTCCAACA GGGTGATTTG GTGTACCTGG 
TTCCTGGACA GCTTCTCTAT CATGTCGATG AGCATCTGCC TGCTGACAAT ATTCATCATG 
CATGGAAGGA TCCTGCATTA CAGCAATCCG TTCATCCTCT TCCTGTTCCT GTTGGCTTTC 
TCCATTGCTA CGATCATGCA GTGCTTCCTG CTCAGCACCT TCTTCTCCAG GGCCAGTCTG 
GCAGCAGCCT GCAGTGGTGT CATCTACTTC ACCCTCTACC TGCCACACAT TCTCTGCTTC 
GCTTGGCAGG ACCGAATTAC AGCCGACATG AAGATGGCTG TGAGCCTGCT GTCTCCTGTG 
GCATTTGGGT TTGGCACTGA GTACCTGGCT CGTTTTGAAG AGCAAGGTGT GGGGCTGCAG 
TGGAGCAATA TTGGGAACAG TCCCATGGAA GGGGATGAAT TCAGTTTTCT GATGTCCATG 
AAGATGATGC TCCTTGATGC TGCTCTGTAT GGCTTGCTTG CCTGGTATCT TGACCAGGTG 
TTCCCAGGGG ACTATGGAAC CCCACTTCCT TGGTACTTCC TTCTACAAGA GTCTTACTGG 
CTAGGTGGTG AAGGCTGTTC AACCAGAGAA GAAAGAGCCT TGGAAAAGAC AGAACCAATA 
ACAGAGGAAA TGGAGGACCC AGAATACCCA GAAGGAATAA ATGACTGCTT CTTTGAACGT 
GAGCTTCCAG GCTTGGTTCC CGGAGTATGT GTGAAGAATC TGGTAAAGAT ATTTGAGCCC 
TATGGCAGGC CAGCCGTGGA CCGTCTTAAC ATTACCTTCT ATGAAAGTCA GATTACCGCA 
TTCCTCGGTC ACAATGGAGC AGGGAAAACC ACGACTTTGT CCATCATGAC CGGTCTGTTG 
CCACCGACAT CCGGGACTGT GCTTGTTGGG GGAAAGGACA TTGAAACCAA CCTGGATGCT 
ATCCGGCAGA GCCTTGGCAT GTGTCCACAG CACAACATCC TGTTCCACCA CCTCACCGTG 
GCTGAACACA TCCTGTTCTA CGCGCAGCTG AAAGGGAGGT CCTGGGACAA GGCCCAGCTG 
GAGATGGAAG CAATGCTGGA GGACACAGGC CTCCACCACA AGAGGAACGA AGAGGCTCAG 
GATCTGTCAG GTGGCGTGCA GAGGAAGCTG TCTGTGGCCA TTGCCTTCGT GGGAGATGCC 
AAGGTGGTGG TTCTGGATGA GCCCACCTCT GGGGTGGACC CCTACTCACG TCGCTCAATC 
TGGGACCTGC TCCTGAAGTA TCGCTCAGGC AGAACCATCA TCATGTCCAC TCACCACATG 
GACGAGGCCG ACATCCTCGG GGACCGCATC GCCATCATAT CCCAGGGACG GCTCTACTGC 
TCTGGAACCC CACTGTTCCT GAAGAACTGC TTTGGCACAG GCTTCTACTT AACCTTGGTG 
CGCAGGATGA AGACTATTCA GAGCCAAGGA AGAGGCCGCG AGGCGACCTG CAGCTGTGCG 
TCTAAGGGCT TCTCCGTCAG GTGTCCGGCC TGTGCCGAGG CGATAACTCC AGAACAAGTC 
TTGGACGGGG ATGTGAATGA ACTGACGGAT ATGGTTCACC ACCATGTTCC AGAGGCGAAG 
CTGGTAGAAT GCATTGGTCA AGAACTTATC TTCCTTCTTC CAAATAAGAA TTTCAAGCAG 
AGAGCATATG CTAGCCTTTT CAGAGAGCTG GAGGAGACGC TGGCTGACCT GGGACTCAGC 
AGTTTTGGAA TTTCTGACAC TCCCCTGGAA GAGATTTTCC TGAAGGTCAC AGAGGATTTG 
GATTCAGGAC ATCTGTTTGC TGGAGGCACT CAGCAGAAAA GAGAAAACAT CAACCTTCGA 
CACCCCTGTT CGGGTCCTAG CGAGAAGGCC GGACAGACTC CCCAGGGCTC CAGCAGCCAC 
CCCAGAGAGC CAGCTGCTCA CCCGGAGGGC CAGCCTCCAC CGGAGCGGGA GGGACACAGC 
CGACTGAACT CAGGAGCTCG GCTTATTGTC CAGCACGTGC AGGCACTGCT GGTCAAGAGG 
TTCCAGCACA CCATCCGCAG CCACAAGGAC TTCCTGGCCC AGATTGTGCT CCCGGCCACT 
TTTGTGTTTT TGGCTCTAAT GCTTTCCCTC ATCATCCCTC CTTTTGGTGA ATATCCTGCT 
TTGACCCTTC ACCCCTGGAT GTATGGACAG CAGTACACCT TCTTCAGCAT GGACCAGCTG 
GACAGTGAAT GGCTCTCAGC ACTTGCAGAT GTCCTTGTGA ATAAGCCAGG CTTTGGCAAC 
CGCTGCCTGA AGGAAGAGTG GCTTCCGGAG TTCCCCTGTG GAAATTCAAG CCCCTGGAAG 
ACTCCATCTG TGTCCCCGGA TGTCACCCAC TTGCTCCAAC AGCAGAAATG GACAGCGGAC 
CAACCTTCAC CTTCCTGCAG GTGCAGCACC AGAGAGAAGC TCACCATGCT TCCTGAGTGC 
CCTGAGGGCG CAGGGGGCCT CCCACCCCCG CAGAGAATAC AACGCAGCAC TGAAATTCTG 
CAGGACCTTA CAGACAGGAA TGTCTCCGAC TTCCTGGTAA AAACATATCC TGCTCTTATC 
CGAAGCAGTT TGAAGAGCAA ATTCTGGGTC AATGAACAGA GGTATGGAGG AATTTCTGTC 
GGAGGAAAGC TCCCAGCTCC CCCCTTCACT GGAGAAGCAC TTGTTGGATT TTTAAGTGAC 
CTTGGCCAGT TAATGAATGT GAGCGGGGGC CCTATGACCA GAGAGGCTGC TAAAGAAATG 
CCTGCTTTCC TTAAACAACT AGAAACTGAA GACAATATTA AGGTGTGGTT TAACAACAAA 
GGTTGGCATG CTCTGGTCAG CTTCCTCAAC GTGGCCCACA ACGCCATCTT ACGGGCTAGC 
CTGCACAAGG ACAAGAACCC CGAGGAGTAT GGCATCACCG TCATAAGCCA GCCCTTGAAC 
CTGACCAAGG AGCAGCTCTC TGAGATCACA GTACTGACCA CTTCGGTGGA CGCTGTAGTG 
GCCATCTGCG TGATTTTTGC CATGTCCTTC GTCCCTGCCA GCTTTGTCCT TTATTTGATC 
CAGGAGAGGG TGAACAAGGC CAAGCACCTC CAGTTTGTCA GCGGAGTGAG CCCCACCACC 
TACTGGCTGA CCAACTTCCT CTGGGACATC ATGAATTACA CTGTAAGTGC TGCACTGGTG 
GTGGGCATCT TCATCGGGTT TCAGAAGAAA GCCTACACTT CTTCAGAGAA CCTTCCAGCC 
CTTGTTGCGC TGCTCATGCT GTATGGGTGG GCAGTCATTC CCATGATGTA TCCGGCATCC 
TTCCTGTTCG ATATCCCCAG CACTGCCTAT GTGGCCTTAT CTTGTGCTAA TCTGTTCATT 
GGCATCAACA GCAGTGCCAT CACTTTCGTC CTGGAATTAT TTGAGAATAA CCGGACGCTG 
CTCAGGATCA ATGCCATGCT GAGGAAGCTG CTCATTATCT TCCCCCACTT CTGCCTGGGC 
CGGGGTCTCA TTGACCTGGC ACTGAGCCAG GCAGTGACAG ACGTCTATGC CCGGTTTGGT 
GAGGAGCACT CTTCAAATCC ATTCCAGTGG GACCTGATTG GGAAGAACCT GGCTGCCATG 
GCAGTAGAAG GTGTGGTGTA CTTCCTCTTG ACGCTCCTCA TCCAATACCA GTTCTTCTTC 
AGCCGATGGA CTACCGAGCC TGCAAAGGAG CCCATCACAG ATGAAGATGA TGATGTGGCT 
GAAGAAAGGC AGAGAATTAT TAGTGGAGGA AATAAAACTG ATATCTTAAG GCTAAATGAA 
CTCACCAAGG TTTATTCAGG CACCTCCAGC CCAGCAGTGG ACAGGCTGTG TGTGGGAGTC 
CGCCCTGGAG AGTGCTTTGG TCTCCTGGGT GTGAACGGAG CTGGCAAAAC CACCACATTC 
AAGATGCTCA CTGGGGACAC CGCAGTGACC TCAGGCGATG CCACTGTCGC AGGCAAGAGT 
ATTTTGACCA ATATTTCTGA CGTCCATCAA AGTATGGGCT ACTGTCCTCA GTTTGATGCA 
ATCGATGACC TGCTTACTGG GCGAGAACAT CTTTACCTTT ATGCCCGGCT TCGAGGTGTA 
CCAGCAGAGG AAATTGAGAG GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010801595.1
CDS	82..6228
Translation	MGFARQIKLLLWKNWTLRKRQKIRFVVELVWPLSLFLVLIWLRNVNPLYSKHECHFPNKAMPSAGMLPWLQGIFCNVNNPCFQSPTAGESPGIVSNYNNSILARVYRDFQELLMDAPESQHLGQVWRELRTLSQLMNTLRMHPERIAGRGIRIREVLKDDEMLTLFLVKNIGLSDSVVYLLVNSQVRPEQFARGVPDLMLKDIACSEALLERFLIFPQRRAAQTVRGSLCSLSQGTLQWMEDTLYANVDFFKLFHVFPRLLDSRSQGMNLRSWGRILSDMSPRIQEFIHRPSVQDLLWVTRPLVQTGGPETFTQLMGILSDLLCGYPEGGGSRVFSFNWYEDNNYKAFLGIDSTRKDPIYSYDERTTTFCNALIQSLESNPLTKIAWRAAKPLLMGKILFTPDSPATRRILKNANSTFEELERVRKLVKVWEEVGPQIWYFFDKSTQMSMIRDTLENPTVKAFWNRQLGEEGITAEAVLNFLYNGPREGQADDVDNFNWRDIFNITDRALRLANQYLECLILDKFESYDDEFQLTQRALSLLEENRFWAGVVFPDMHPWTSSLPPHVKYKIRMDIDVVEKTNKIKDRYWDSGPRADPVEDFRYIWGGFAYLQDMVEHGITRSQAQEEVPVGIYLQQMPYPCFVDDSFMIILNRCFPIFMVLAWIYSVSMTVKSIVLEKELRLKETLKNQGVSNRVIWCTWFLDSFSIMSMSICLLTIFIMHGRILHYSNPFILFLFLLAFSIATIMQCFLLSTFFSRASLAAACSGVIYFTLYLPHILCFAWQDRITADMKMAVSLLSPVAFGFGTEYLARFEEQGVGLQWSNIGNSPMEGDEFSFLMSMKMMLLDAALYGLLAWYLDQVFPGDYGTPLPWYFLLQESYWLGGEGCSTREERALEKTEPITEEMEDPEYPEGINDCFFERELPGLVPGVCVKNLVKIFEPYGRPAVDRLNITFYESQITAFLGHNGAGKTTTLSIMTGLLPPTSGTVLVGGKDIETNLDAIRQSLGMCPQHNILFHHLTVAEHILFYAQLKGRSWDKAQLEMEAMLEDTGLHHKRNEEAQDLSGGVQRKLSVAIAFVGDAKVVVLDEPTSGVDPYSRRSIWDLLLKYRSGRTIIMSTHHMDEADILGDRIAIISQGRLYCSGTPLFLKNCFGTGFYLTLVRRMKTIQSQGRGREATCSCASKGFSVRCPACAEAITPEQVLDGDVNELTDMVHHHVPEAKLVECIGQELIFLLPNKNFKQRAYASLFRELEETLADLGLSSFGISDTPLEEIFLKVTEDLDSGHLFAGGTQQKRENINLRHPCSGPSEKAGQTPQGSSSHPREPAAHPEGQPPPEREGHSRLNSGARLIVQHVQALLVKRFQHTIRSHKDFLAQIVLPATFVFLALMLSLIIPPFGEYPALTLHPWMYGQQYTFFSMDQLDSEWLSALADVLVNKPGFGNRCLKEEWLPEFPCGNSSPWKTPSVSPDVTHLLQQQKWTADQPSPSCRCSTREKLTMLPECPEGAGGLPPPQRIQRSTEILQDLTDRNVSDFLVKTYPALIRSSLKSKFWVNEQRYGGISVGGKLPAPPFTGEALVGFLSDLGQLMNVSGGPMTREAAKEMPAFLKQLETEDNIKVWFNNKGWHALVSFLNVAHNAILRASLHKDKNPEEYGITVISQPLNLTKEQLSEITVLTTSVDAVVAICVIFAMSFVPASFVLYLIQERVNKAKHLQFVSGVSPTTYWLTNFLWDIMNYTVSAALVVGIFIGFQKKAYTSSENLPALVALLMLYGWAVIPMMYPASFLFDIPSTAYVALSCANLFIGINSSAITFVLELFENNRTLLRINAMLRKLLIIFPHFCLGRGLIDLALSQAVTDVYARFGEEHSSNPFQWDLIGKNLAAMAVEGVVYFLLTLLIQYQFFFSRWTTEPAKEPITDEDDDVAEERQRIISGGNKTDILRLNELTKVYSGTSSPAVDRLCVGVRPGECFGLLGVNGAGKTTTFKMLTGDTAVTSGDATVAGKSILTNISDVHQSMGYCPQFDAIDDLLTGREHLYLYARLRGVPAEEIERD

Target ORF information:

RefSeq Version	XM_010803293.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ATP binding cassette subfamily A member 4 (ABCA4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010803293.2

ORF Insert Sequence:

ATGGGCTTTG CCAGACAGAT CAAGCTTCTG CTGTGGAAGA ACTGGACCCT GCGGAAAAGG 
CAAAAGATTC GCTTTGTGGT GGAACTCGTG TGGCCTTTAT CTTTATTTCT GGTCTTGATC 
TGGTTAAGGA ATGTCAACCC CCTCTACAGC AAACATGAAT GCCATTTTCC CAACAAGGCG 
ATGCCCTCAG CAGGAATGTT GCCATGGCTC CAGGGAATTT TCTGCAATGT GAACAATCCT 
TGTTTTCAAA GTCCCACTGC AGGAGAATCT CCTGGAATTG TGTCAAACTA TAACAACTCC 
ATCTTGGCAA GGGTGTATAG AGATTTTCAA GAACTCCTCA TGGATGCACC AGAGAGCCAG 
CACCTTGGTC AGGTTTGGAG AGAACTCCGC ACCTTGTCCC AGCTCATGAA CACCCTCCGA 
ATGCACCCCG AGAGGATTGC AGGAAGAGGA ATACGAATTC GGGAGGTCCT AAAAGATGAT 
GAAATGTTGA CACTGTTTCT CGTGAAGAAC ATTGGTCTGT CTGACTCAGT CGTCTACCTT 
CTGGTCAACT CCCAAGTCCG TCCAGAGCAG TTTGCTCGTG GAGTCCCAGA CCTGATGCTG 
AAGGACATCG CCTGCAGTGA GGCCCTCCTG GAGCGTTTCC TGATCTTTCC CCAGCGCCGT 
GCAGCCCAGA CAGTGCGTGG CAGCTTGTGC TCCCTCTCTC AGGGAACCCT ACAGTGGATG 
GAAGACACCC TGTACGCCAA CGTGGACTTC TTCAAGCTCT TCCATGTGTT TCCCAGACTC 
CTAGACAGCC GTTCTCAAGG TATGAATCTG AGATCCTGGG GAAGAATATT ATCTGATATG 
TCACCAAGAA TTCAAGAATT CATCCACCGG CCAAGTGTTC AAGACTTGCT GTGGGTGACC 
AGGCCACTCG TGCAGACTGG TGGCCCGGAG ACCTTCACGC AGCTGATGGG CATCCTGTCT 
GACCTCTTGT GTGGCTACCC CGAGGGAGGA GGATCTCGGG TGTTCTCCTT CAACTGGTAT 
GAAGACAATA ACTATAAGGC CTTTCTAGGG ATCGACTCCA CAAGGAAAGA TCCTATCTAT 
TCTTATGATG AAAGAACAAC AACCTTTTGT AACGCATTGA TCCAGAGCCT GGAGTCAAAC 
CCTCTAACCA AAATAGCCTG GAGGGCAGCA AAGCCTTTGT TGATGGGAAA AATCCTCTTT 
ACTCCAGATT CCCCTGCAAC ACGCAGGATA CTGAAGAATG CTAATTCGAC GTTTGAAGAG 
CTGGAGCGTG TCAGGAAGTT GGTCAAAGTC TGGGAAGAAG TAGGGCCCCA GATCTGGTAC 
TTCTTTGACA AGAGCACACA GATGAGCATG ATCAGAGACA CTCTGGAGAA CCCAACAGTA 
AAAGCTTTTT GGAATAGGCA GCTTGGTGAG GAAGGCATCA CTGCTGAAGC CGTCCTGAAC 
TTCCTCTATA ACGGGCCTCG AGAGGGCCAG GCTGACGATG TGGACAACTT CAACTGGAGG 
GATATATTTA ACATCACTGA TCGTGCCCTA CGCCTGGCTA ATCAATATCT GGAGTGCTTG 
ATCCTGGATA AGTTTGAAAG CTACGATGAT GAATTTCAAC TCACTCAGCG AGCCCTGTCC 
CTGCTGGAGG AAAACAGGTT CTGGGCTGGA GTGGTATTTC CGGACATGCA CCCCTGGACC 
AGTTCCTTAC CACCCCATGT GAAGTACAAG ATCCGAATGG ACATAGATGT GGTGGAGAAA 
ACCAATAAGA TCAAAGACAG GTACTGGGAT TCTGGCCCCA GAGCTGATCC TGTGGAAGAC 
TTCCGATACA TCTGGGGAGG GTTTGCCTAT CTGCAGGACA TGGTTGAACA TGGGATCACG 
AGGAGTCAGG CCCAGGAGGA GGTTCCGGTT GGAATCTATC TCCAGCAGAT GCCTTACCCC 
TGCTTCGTGG ATGACTCTTT TATGATCATC CTGAACCGCT GTTTCCCTAT CTTCATGGTG 
CTGGCCTGGA TCTACTCTGT CTCCATGACT GTAAAGAGCA TTGTCTTGGA GAAGGAGTTG 
AGACTGAAGG AAACCTTGAA AAATCAGGGT GTCTCCAACA GGGTGATTTG GTGTACCTGG 
TTCCTGGACA GCTTCTCTAT CATGTCGATG AGCATCTGCC TGCTGACAAT ATTCATCATG 
CATGGAAGGA TCCTGCATTA CAGCAATCCG TTCATCCTCT TCCTGTTCCT GTTGGCTTTC 
TCCATTGCTA CGATCATGCA GTGCTTCCTG CTCAGCACCT TCTTCTCCAG GGCCAGTCTG 
GCAGCAGCCT GCAGTGGTGT CATCTACTTC ACCCTCTACC TGCCACACAT TCTCTGCTTC 
GCTTGGCAGG ACCGAATTAC AGCCGACATG AAGATGGCTG TGAGCCTGCT GTCTCCTGTG 
GCATTTGGGT TTGGCACTGA GTACCTGGCT CGTTTTGAAG AGCAAGGTGT GGGGCTGCAG 
TGGAGCAATA TTGGGAACAG TCCCATGGAA GGGGATGAAT TCAGTTTTCT GATGTCCATG 
AAGATGATGC TCCTTGATGC TGCTCTGTAT GGCTTGCTTG CCTGGTATCT TGACCAGGTG 
TTCCCAGGGG ACTATGGAAC CCCACTTCCT TGGTACTTCC TTCTACAAGA GTCTTACTGG 
CTAGGTGGTG AAGGCTGTTC AACCAGAGAA GAAAGAGCCT TGGAAAAGAC AGAACCAATA 
ACAGAGGAAA TGGAGGACCC AGAATACCCA GAAGGAATAA ATGACTGCTT CTTTGAACGT 
GAGCTTCCAG GCTTGGTTCC CGGAGTATGT GTGAAGAATC TGGTAAAGAT ATTTGAGCCC 
TATGGCAGGC CAGCCGTGGA CCGTCTTAAC ATTACCTTCT ATGAAAGTCA GATTACCGCA 
TTCCTCGGTC ACAATGGAGC AGGGAAAACC ACGACTTTGT CCATCATGAC CGGTCTGTTG 
CCACCGACAT CCGGGACTGT GCTTGTTGGG GGAAAGGACA TTGAAACCAA CCTGGATGCT 
ATCCGGCAGA GCCTTGGCAT GTGTCCACAG CACAACATCC TGTTCCACCA CCTCACCGTG 
GCTGAACACA TCCTGTTCTA CGCGCAGCTG AAAGGGAGGT CCTGGGACAA GGCCCAGCTG 
GAGATGGAAG CAATGCTGGA GGACACAGGC CTCCACCACA AGAGGAACGA AGAGGCTCAG 
GATCTGTCAG GTGGCGTGCA GAGGAAGCTG TCTGTGGCCA TTGCCTTCGT GGGAGATGCC 
AAGGTGGTGG TTCTGGATGA GCCCACCTCT GGGGTGGACC CCTACTCACG TCGCTCAATC 
TGGGACCTGC TCCTGAAGTA TCGCTCAGGC AGAACCATCA TCATGTCCAC TCACCACATG 
GACGAGGCCG ACATCCTCGG GGACCGCATC GCCATCATAT CCCAGGGACG GCTCTACTGC 
TCTGGAACCC CACTGTTCCT GAAGAACTGC TTTGGCACAG GCTTCTACTT AACCTTGGTG 
CGCAGGATGA AGACTATTCA GAGCCAAGGA AGAGGCCGCG AGGCGACCTG CAGCTGTGCG 
TCTAAGGGCT TCTCCGTCAG GTGTCCGGCC TGTGCCGAGG CGATAACTCC AGAACAAGTC 
TTGGACGGGG ATGTGAATGA ACTGACGGAT ATGGTTCACC ACCATGTTCC AGAGGCGAAG 
CTGGTAGAAT GCATTGGTCA AGAACTTATC TTCCTTCTTC CAAATAAGAA TTTCAAGCAG 
AGAGCATATG CTAGCCTTTT CAGAGAGCTG GAGGAGACGC TGGCTGACCT GGGACTCAGC 
AGTTTTGGAA TTTCTGACAC TCCCCTGGAA GAGATTTTCC TGAAGGTCAC AGAGGATTTG 
GATTCAGGAC ATCTGTTTGC TGGAGGCACT CAGCAGAAAA GAGAAAACAT CAACCTTCGA 
CACCCCTGTT CGGGTCCTAG CGAGAAGGCC GGACAGACTC CCCAGGGCTC CAGCAGCCAC 
CCCAGAGAGC CAGCTGCTCA CCCGGAGGGC CAGCCTCCAC CGGAGCGGGA GGGACACAGC 
CGACTGAACT CAGGAGCTCG GCTTATTGTC CAGCACGTGC AGGCACTGCT GGTCAAGAGG 
TTCCAGCACA CCATCCGCAG CCACAAGGAC TTCCTGGCCC AGATTGTGCT CCCGGCCACT 
TTTGTGTTTT TGGCTCTAAT GCTTTCCCTC ATCATCCCTC CTTTTGGTGA ATATCCTGCT 
TTGACCCTTC ACCCCTGGAT GTATGGACAG CAGTACACCT TCTTCAGCAT GGACCAGCTG 
GACAGTGAAT GGCTCTCAGC ACTTGCAGAT GTCCTTGTGA ATAAGCCAGG CTTTGGCAAC 
CGCTGCCTGA AGGAAGAGTG GCTTCCGGAG TTCCCCTGTG GAAATTCAAG CCCCTGGAAG 
ACTCCATCTG TGTCCCCGGA TGTCACCCAC TTGCTCCAAC AGCAGAAATG GACAGCGGAC 
CAACCTTCAC CTTCCTGCAG GTGCAGCACC AGAGAGAAGC TCACCATGCT TCCTGAGTGC 
CCTGAGGGCG CAGGGGGCCT CCCACCCCCG CAGAGAATAC AACGCAGCAC TGAAATTCTG 
CAGGACCTTA CAGACAGGAA TGTCTCCGAC TTCCTGGTAA AAACATATCC TGCTCTTATC 
CGAAGCAGTT TGAAGAGCAA ATTCTGGGTC AATGAACAGA GGTATGGAGG AATTTCTGTC 
GGAGGAAAGC TCCCAGCTCC CCCCTTCACT GGAGAAGCAC TTGTTGGATT TTTAAGTGAC 
CTTGGCCAGT TAATGAATGT GAGCGGGGGC CCTATGACCA GAGAGGCTGC TAAAGAAATG 
CCTGCTTTCC TTAAACAACT AGAAACTGAA GACAATATTA AGGTGTGGTT TAACAACAAA 
GGTTGGCATG CTCTGGTCAG CTTCCTCAAC GTGGCCCACA ACGCCATCTT ACGGGCTAGC 
CTGCACAAGG ACAAGAACCC CGAGGAGTAT GGCATCACCG TCATAAGCCA GCCCTTGAAC 
CTGACCAAGG AGCAGCTCTC TGAGATCACA GTACTGACCA CTTCGGTGGA CGCTGTAGTG 
GCCATCTGCG TGATTTTTGC CATGTCCTTC GTCCCTGCCA GCTTTGTCCT TTATTTGATC 
CAGGAGAGGG TGAACAAGGC CAAGCACCTC CAGTTTGTCA GCGGAGTGAG CCCCACCACC 
TACTGGCTGA CCAACTTCCT CTGGGACATC ATGAATTACA CTGTAAGTGC TGCACTGGTG 
GTGGGCATCT TCATCGGGTT TCAGAAGAAA GCCTACACTT CTTCAGAGAA CCTTCCAGCC 
CTTGTTGCGC TGCTCATGCT GTATGGGTGG GCAGTCATTC CCATGATGTA TCCGGCATCC 
TTCCTGTTCG ATATCCCCAG CACTGCCTAT GTGGCCTTAT CTTGTGCTAA TCTGTTCATT 
GGCATCAACA GCAGTGCCAT CACTTTCGTC CTGGAATTAT TTGAGAATAA CCGGACGCTG 
CTCAGGATCA ATGCCATGCT GAGGAAGCTG CTCATTATCT TCCCCCACTT CTGCCTGGGC 
CGGGGTCTCA TTGACCTGGC ACTGAGCCAG GCAGTGACAG ACGTCTATGC CCGGTTTGGT 
GAGGAGCACT CTTCAAATCC ATTCCAGTGG GACCTGATTG GGAAGAACCT GGCTGCCATG 
GCAGTAGAAG GTGTGGTGTA CTTCCTCTTG ACGCTCCTCA TCCAATACCA GTTCTTCTTC 
AGCCGATGGA CTACCGAGCC TGCAAAGGAG CCCATCACAG ATGAAGATGA TGATGTGGCT 
GAAGAAAGGC AGAGAATTAT TAGTGGAGGA AATAAAACTG ATATCTTAAG GCTAAATGAA 
CTCACCAAGG TTTATTCAGG CACCTCCAGC CCAGCAGTGG ACAGGCTGTG TGTGGGAGTC 
CGCCCTGGAG AGTGCTTTGG TCTCCTGGGT GTGAACGGAG CTGGCAAAAC CACCACATTC 
AAGATGCTCA CTGGGGACAC CGCAGTGACC TCAGGCGATG CCACTGTCGC AGGCAAGAGT 
ATTTTGACCA ATATTTCTGA CGTCCATCAA AGTATGGGCT ACTGTCCTCA GTTTGATGCA 
ATCGATGACC TGCTTACTGG GCGAGAACAT CTTTACCTTT ATGCCCGGCT TCGAGGTGTA 
CCAGCAGAGG AAATTGAGAG GGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ATP-binding cassette transporter ABCA4 and chemical isomerization protect photoreceptor cells from the toxic accumulation of excess 11-cis-retinal.
Proceedings of the National Academy of Sciences of the United States of America111(13)5024-9(2014 Apr)
Quazi F,Molday RS

Molecular organization and ATP-induced conformational changes of ABCA4, the photoreceptor-specific ABC transporter.
Structure (London, England : 1993)21(5)854-60(2013 May)
Tsybovsky Y,Orban T,Molday RS,Taylor D,Palczewski K

Posttranslational modifications of the photoreceptor-specific ABC transporter ABCA4.
Biochemistry50(32)6855-66(2011 Aug)
Tsybovsky Y,Wang B,Quazi F,Molday RS,Palczewski K

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

N-retinylidene-phosphatidylethanolamine is the preferred retinoid substrate for the photoreceptor-specific ABC transporter ABCA4 (ABCR).
The Journal of biological chemistry279(52)53972-9(2004 Dec)
Beharry S,Zhong M,Molday RS