LGMN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LGMN
Entrez Gene ID	281281
Full Name	legumain
General protein information	Preferred Names legumain Names legumain asparaginyl endopeptidase protease, cysteine 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
NM_174101.2	NP_776526.1	legumain precursor
XM_024981595.1	XP_024837363.1	legumain isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LGMN	NP_776526.1
Rattus norvegicus (Norway rat)	Lgmn	NP_071562.2
Homo sapiens (human)	LGMN	NP_001008530.1
Xenopus tropicalis (tropical clawed frog)	lgmn	NP_001005720.1
	LGMN	XP_001092047.1
Mus musculus (house mouse)	Lgmn	NP_035305.1
Pan troglodytes (chimpanzee)	LGMN	XP_001148301.1
Canis lupus familiaris (dog)	LGMN	XP_537355.2
Gallus gallus (chicken)	LGMN	XP_421328.1
Danio rerio (zebrafish)	lgmn	NP_999924.1
Caenorhabditis elegans (roundworm)	T28H10.3	NP_506137.1
Arabidopsis thaliana (thale cress)	DELTA-VPE	NP_188656.1

Gene Ontology
Bibliography

Related articles in PubMed

Bovine endometrial legumain and TIMP-2 regulation in response to presence of a conceptus.
Ledgard AM, Lee RS, Peterson AJ
Molecular reproduction and development76(1)65-74(2009 Jan)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Legumain from bovine kidney: its purification, molecular cloning, immunohistochemical localization and degradation of annexin II and vitamin D-binding protein.
Yamane T, Takeuchi K, Yamamoto Y, Li YH, Fujiwara M, Nishi K, Takahashi S, Ohkubo I
Biochimica et biophysica acta1596(1)108-20(2002 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

legumain and TIMP-2 mRNAs were up-regulated in the endometrium during the luteal phase of the oestrous cycle and during early pregnancy
Title: Bovine endometrial legumain and TIMP-2 regulation in response to presence of a conceptus.

The following LGMN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LGMN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41595	NM_174101.2 Latest version!	Bos taurus legumain (LGMN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OBa228207	XM_024981595.1 Latest version!	Bos taurus legumain (LGMN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LGMN ( NM_174101.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776526.1 Bos taurus legumain (LGMN), mRNA.

Price & Availability↑

CloneID	OBa41595
Clone ID Related Accession (Same CDS sequence)	NM_174101.2
Accession Version	NM_174101.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	legumain precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB060129.1. On or before Dec 7, 2011 this sequence version replaced XM_003585053.1, NM_174101.1. ##Evidence-Data-START## Transcript exon combination :: AB060129.1, BC111117.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGATTTGGG AATTCACCGT GTTGCTCAGC TTGGTCCTGG GAACTGGTGC TGTCCCCCTG 
GAGGACCCTG AGGACGGAGG CAAGCACTGG GTGGTGATCG TCGCAGGATC AAACGGCTGG 
TATAATTACA GGCACCAGGC AGATGCATGC CATGCCTACC AGATCGTTCA CCGAAACGGG 
ATCCCTGATG AGCAGATCAT CGTGATGATG TATGACGACA TCGCCAACTC CGAAGACAAT 
CCCACCCCGG GAATTGTGAT CAACAGGCCC AACGGCTCAG ACGTGTACCA GGGCGTGCTG 
AAGGACTACA CGGGCGAGGA TGTCACCCCG AAGAATTTCC TTGCTGTGTT GAGAGGTGAT 
GCAGAAGCAG TGAAGGGCGT AGGATCTGGA AAGGTCTTAA AGAGTGGCCC CAGGGACCAC 
GTGTTCGTTT ACTTCACGGA TCATGGAGCC ACTGGAATAC TGGTGTTTCC TAATGAAGAT 
CTTCATGTAA AGGACCTGAA TGAGACCATC CGTTACATGT ACGAACACAA GATGTACCAA 
AAGATGGTGT TCTACATTGA GGCCTGTGAG TCTGGGTCCA TGATGAACCA CCTGCCACCC 
GACATCAATG TTTATGCAAC TACGGCTGCC AACCCCAGGG AGTCGTCCTA CGCCTGTTAC 
TACGATGAGC AGCGGTCCAC GTTCCTGGGG GACTGGTACA GCGTCAACTG GATGGAAGAT 
TCAGATGTGG AGGATTTGAC CAAAGAGACC CTCCACAAGC AGTACCAGCT GGTGAAATCC 
CACACCAACA CCAGCCACGT CATGCAGTAT GGGAACAAGT CCATCTCTGC CATGAAGCTG 
ATGCAGTTTC AGGGTCTGAA ACACCAAGCT AGCTCTCCCA TCTCGCTGCC CGCTGTCAGT 
CGCCTTGACC TCACTCCAAG CCCCGAGGTG CCGCTCAGCA TCATGAAAAG GAAGCTGATG 
AGCACCAACG ATCTGCAGGA GTCCAGGCGT CTTGTCCAGA AGATCGACAG GCATCTGGAG 
GCCAGGAACA TCATTGAGAA GTCAGTGCGA AAGATCGTCA CCTTGGTCTC GGGCTCCGCT 
GCCGAGGTAG ACAGACTCCT GTCCCAGAGA GCCCCGCTCA CAGAGCACGC ATGCTACCAG 
ACGGCCGTGT CGCACTTCCG CTCACACTGC TTCAACTGGC ACAACCCCAC GTACGAGTAT 
GCTTTGAGAC ATTTGTACGT GCTTGTCAAC CTTTGTGAAA ACCCTTATCC AATTGACAGA 
ATAAAATTGT CCATGAACAA GGTGTGCCAT GGCTACTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776526.1
CDS	88..1389
Translation	MIWEFTVLLSLVLGTGAVPLEDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNGIPDEQIIVMMYDDIANSEDNPTPGIVINRPNGSDVYQGVLKDYTGEDVTPKNFLAVLRGDAEAVKGVGSGKVLKSGPRDHVFVYFTDHGATGILVFPNEDLHVKDLNETIRYMYEHKMYQKMVFYIEACESGSMMNHLPPDINVYATTAANPRESSYACYYDEQRSTFLGDWYSVNWMEDSDVEDLTKETLHKQYQLVKSHTNTSHVMQYGNKSISAMKLMQFQGLKHQASSPISLPAVSRLDLTPSPEVPLSIMKRKLMSTNDLQESRRLVQKIDRHLEARNIIEKSVRKIVTLVSGSAAEVDRLLSQRAPLTEHACYQTAVSHFRSHCFNWHNPTYEYALRHLYVLVNLCENPYPIDRIKLSMNKVCHGYY

Target ORF information:

RefSeq Version	NM_174101.2
Organism	Bos taurus(cattle)
Definition	Bos taurus legumain (LGMN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174101.2

ORF Insert Sequence:

ATGATTTGGG AATTCACCGT GTTGCTCAGC TTGGTCCTGG GAACTGGTGC TGTCCCCCTG 
GAGGACCCTG AGGACGGAGG CAAGCACTGG GTGGTGATCG TCGCAGGATC AAACGGCTGG 
TATAATTACA GGCACCAGGC AGATGCATGC CATGCCTACC AGATCGTTCA CCGAAACGGG 
ATCCCTGATG AGCAGATCAT CGTGATGATG TATGACGACA TCGCCAACTC CGAAGACAAT 
CCCACCCCGG GAATTGTGAT CAACAGGCCC AACGGCTCAG ACGTGTACCA GGGCGTGCTG 
AAGGACTACA CGGGCGAGGA TGTCACCCCG AAGAATTTCC TTGCTGTGTT GAGAGGTGAT 
GCAGAAGCAG TGAAGGGCGT AGGATCTGGA AAGGTCTTAA AGAGTGGCCC CAGGGACCAC 
GTGTTCGTTT ACTTCACGGA TCATGGAGCC ACTGGAATAC TGGTGTTTCC TAATGAAGAT 
CTTCATGTAA AGGACCTGAA TGAGACCATC CGTTACATGT ACGAACACAA GATGTACCAA 
AAGATGGTGT TCTACATTGA GGCCTGTGAG TCTGGGTCCA TGATGAACCA CCTGCCACCC 
GACATCAATG TTTATGCAAC TACGGCTGCC AACCCCAGGG AGTCGTCCTA CGCCTGTTAC 
TACGATGAGC AGCGGTCCAC GTTCCTGGGG GACTGGTACA GCGTCAACTG GATGGAAGAT 
TCAGATGTGG AGGATTTGAC CAAAGAGACC CTCCACAAGC AGTACCAGCT GGTGAAATCC 
CACACCAACA CCAGCCACGT CATGCAGTAT GGGAACAAGT CCATCTCTGC CATGAAGCTG 
ATGCAGTTTC AGGGTCTGAA ACACCAAGCT AGCTCTCCCA TCTCGCTGCC CGCTGTCAGT 
CGCCTTGACC TCACTCCAAG CCCCGAGGTG CCGCTCAGCA TCATGAAAAG GAAGCTGATG 
AGCACCAACG ATCTGCAGGA GTCCAGGCGT CTTGTCCAGA AGATCGACAG GCATCTGGAG 
GCCAGGAACA TCATTGAGAA GTCAGTGCGA AAGATCGTCA CCTTGGTCTC GGGCTCCGCT 
GCCGAGGTAG ACAGACTCCT GTCCCAGAGA GCCCCGCTCA CAGAGCACGC ATGCTACCAG 
ACGGCCGTGT CGCACTTCCG CTCACACTGC TTCAACTGGC ACAACCCCAC GTACGAGTAT 
GCTTTGAGAC ATTTGTACGT GCTTGTCAAC CTTTGTGAAA ACCCTTATCC AATTGACAGA 
ATAAAATTGT CCATGAACAA GGTGTGCCAT GGCTACTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LGMN ( XM_024981595.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837363.1 Bos taurus legumain (LGMN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa228207
Clone ID Related Accession (Same CDS sequence)	XM_024981595.1
Accession Version	XM_024981595.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1302bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	legumain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037348.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTGGG AAGTCACCGT GTTGCTCAGC TTGGTCCTGG GAACTGGTGC TGTCCCCCTG 
GAGGACCCTG AGGACGGAGG CAAGCACTGG GTGGTGATCG TCGCAGGATC AAATGGCTGG 
TATAATTACA GGCACCAGGC AGATGCATGC CATGCCTACC AGATCGTTCA CCGAAACGGG 
ATCCCTGATG AGCAGATCAT CGTGATGATG TATGACGACA TCGCCAACTC CGAAGACAAT 
CCCACCCCGG GAATTGTGAT CAACAGGCCC AACGGCTCAG ACGTGTACCA GGGCGTACTG 
AAGGACTACA CGGGCGAGGA TGTCACCCCG AAGAATTTCC TTGCTGTGTT GAGAGGTGAT 
GCAGAAGCAG TGAAGGGCGT AGGATCTGGA AAGGTCTTAA AGAGTGGCCC CAGGGACCAC 
GTGTTCGTTT ACTTCACGGA TCATGGAGCC ACTGGAATAC TGGTGTTTCC TAATGAAGAT 
CTTCATGTAA AGGACCTGAA TGAGACCATC CGTTACATGT ACGAACACAA GATGTACCAA 
AAGATGGTGT TCTACATTGA GGCCTGTGAG TCTGGGTCCA TGATGAACCA CCTGCCACCC 
GACATCAATG TTTATGCAAC TACGGCTGCC AACCCCAGGG AGTCGTCCTA CGCCTGTTAC 
TACGATGAGC AGCGGTCCAC GTTCCTGGGG GACTGGTACA GCGTCAACTG GATGGAAGAT 
TCAGATGTGG AGGATTTGAC CAAAGAGACC CTCCACAAGC AGTACCAGCT GGTGAAATCC 
CACACCAACA CCAGCCACGT CATGCAGTAT GGGAACAAGT CCATCTCTGC CATGAAGCTG 
ATGCAGTTTC AGGGTCTGAA ACACCAAGCT AGCTCTCCCA TCTCGCTGCC CGCTGTCAGT 
CGCCTTGACC TCACTCCAAG CCCCGAGGTG CCGCTCAGCA TCATGAAAAG GAAGCTGATG 
AGCACCAACG ATCTGCAGGA GTCCAGGCGT CTTGTCCAGA AGATCGACAG GCATCTGGAG 
GCCAGGAACA TCATTGAGAA GTCAGTGCGA AAGATCGTCA CCTTGGTCTC GGGCTCCGCT 
GCCGAGGTAG ACAGACTCCT GTCCCAGAGA GCCCCGCTCA CAGAGCACGC ATGCTACCAG 
ACGGCCGTGT CGCACTTCCG CTCACACTGC TTCAACTGGC ACAACCCCAC GTACGAGTAT 
GCTTTGAGAC ATTTGTACGT GCTTGTCAAC CTTTGTGAAA ACCCTTATCC AATTGACAGA 
ATAAAATTGT CCATGAACAA GGTGTGCCAT GGCTACTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837363.1
CDS	260..1561
Translation	MIWEVTVLLSLVLGTGAVPLEDPEDGGKHWVVIVAGSNGWYNYRHQADACHAYQIVHRNGIPDEQIIVMMYDDIANSEDNPTPGIVINRPNGSDVYQGVLKDYTGEDVTPKNFLAVLRGDAEAVKGVGSGKVLKSGPRDHVFVYFTDHGATGILVFPNEDLHVKDLNETIRYMYEHKMYQKMVFYIEACESGSMMNHLPPDINVYATTAANPRESSYACYYDEQRSTFLGDWYSVNWMEDSDVEDLTKETLHKQYQLVKSHTNTSHVMQYGNKSISAMKLMQFQGLKHQASSPISLPAVSRLDLTPSPEVPLSIMKRKLMSTNDLQESRRLVQKIDRHLEARNIIEKSVRKIVTLVSGSAAEVDRLLSQRAPLTEHACYQTAVSHFRSHCFNWHNPTYEYALRHLYVLVNLCENPYPIDRIKLSMNKVCHGYY

Target ORF information:

RefSeq Version	XM_024981595.1
Organism	Bos taurus(cattle)
Definition	Bos taurus legumain (LGMN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981595.1

ORF Insert Sequence:

ATGATTTGGG AAGTCACCGT GTTGCTCAGC TTGGTCCTGG GAACTGGTGC TGTCCCCCTG 
GAGGACCCTG AGGACGGAGG CAAGCACTGG GTGGTGATCG TCGCAGGATC AAATGGCTGG 
TATAATTACA GGCACCAGGC AGATGCATGC CATGCCTACC AGATCGTTCA CCGAAACGGG 
ATCCCTGATG AGCAGATCAT CGTGATGATG TATGACGACA TCGCCAACTC CGAAGACAAT 
CCCACCCCGG GAATTGTGAT CAACAGGCCC AACGGCTCAG ACGTGTACCA GGGCGTACTG 
AAGGACTACA CGGGCGAGGA TGTCACCCCG AAGAATTTCC TTGCTGTGTT GAGAGGTGAT 
GCAGAAGCAG TGAAGGGCGT AGGATCTGGA AAGGTCTTAA AGAGTGGCCC CAGGGACCAC 
GTGTTCGTTT ACTTCACGGA TCATGGAGCC ACTGGAATAC TGGTGTTTCC TAATGAAGAT 
CTTCATGTAA AGGACCTGAA TGAGACCATC CGTTACATGT ACGAACACAA GATGTACCAA 
AAGATGGTGT TCTACATTGA GGCCTGTGAG TCTGGGTCCA TGATGAACCA CCTGCCACCC 
GACATCAATG TTTATGCAAC TACGGCTGCC AACCCCAGGG AGTCGTCCTA CGCCTGTTAC 
TACGATGAGC AGCGGTCCAC GTTCCTGGGG GACTGGTACA GCGTCAACTG GATGGAAGAT 
TCAGATGTGG AGGATTTGAC CAAAGAGACC CTCCACAAGC AGTACCAGCT GGTGAAATCC 
CACACCAACA CCAGCCACGT CATGCAGTAT GGGAACAAGT CCATCTCTGC CATGAAGCTG 
ATGCAGTTTC AGGGTCTGAA ACACCAAGCT AGCTCTCCCA TCTCGCTGCC CGCTGTCAGT 
CGCCTTGACC TCACTCCAAG CCCCGAGGTG CCGCTCAGCA TCATGAAAAG GAAGCTGATG 
AGCACCAACG ATCTGCAGGA GTCCAGGCGT CTTGTCCAGA AGATCGACAG GCATCTGGAG 
GCCAGGAACA TCATTGAGAA GTCAGTGCGA AAGATCGTCA CCTTGGTCTC GGGCTCCGCT 
GCCGAGGTAG ACAGACTCCT GTCCCAGAGA GCCCCGCTCA CAGAGCACGC ATGCTACCAG 
ACGGCCGTGT CGCACTTCCG CTCACACTGC TTCAACTGGC ACAACCCCAC GTACGAGTAT 
GCTTTGAGAC ATTTGTACGT GCTTGTCAAC CTTTGTGAAA ACCCTTATCC AATTGACAGA 
ATAAAATTGT CCATGAACAA GGTGTGCCAT GGCTACTACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bovine endometrial legumain and TIMP-2 regulation in response to presence of a conceptus.
Molecular reproduction and development76(1)65-74(2009 Jan)
Ledgard AM,Lee RS,Peterson AJ

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Legumain from bovine kidney: its purification, molecular cloning, immunohistochemical localization and degradation of annexin II and vitamin D-binding protein.
Biochimica et biophysica acta1596(1)108-20(2002 Apr)
Yamane T,Takeuchi K,Yamamoto Y,Li YH,Fujiwara M,Nishi K,Takahashi S,Ohkubo I