KRT5 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	KRT5
Entrez Gene ID	281268
Full Name	keratin 5
General protein information	Preferred Names keratin 5 Names keratin, type II cytoskeletal 5 CK-5 K5 cytokeratin-5 keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types) keratin, type II cytoskeletal 60 kDa, component III type-II keratin Kb5
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 5 Map Location: 5q14-q23

mRNA and Protein(s)

mRNA	Protein	Name
NM_001008663.1	NP_001008663.1	keratin, type II cytoskeletal 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	KRT5	NP_000415.2
	LOC100424400	XP_002798641.1
Canis lupus familiaris (dog)	KRT5	XP_005636850.1
Gallus gallus (chicken)	KRT5	NP_001001195.1
Mus musculus (house mouse)	Krt5	NP_081287.1
Rattus norvegicus (Norway rat)	Krt5	NP_899162.1
Xenopus tropicalis (tropical clawed frog)	krt5.2	NP_001072377.1
Bos taurus (cattle)	KRT5	NP_001008663.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of Intrauterine Growth Restriction During Late Pregnancy on the Development of the Ovine Fetal Thymus and the T-Lymphocyte Subpopulation.
Liu Y, He S, Zhang Y, Xia W, Li M, Zhang C, Gao F
American journal of reproductive immunology (New York, N.Y. : 1989)74(1)26-37(2015 Jul)

Keratin 5-Cre-driven excision of nonmuscle myosin IIA in early embryo trophectoderm leads to placenta defects and embryonic lethality.
Crish J, Conti MA, Sakai T, Adelstein RS, Egelhoff TT
Developmental biology382(1)136-48(2013 Oct)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

A mutation in bovine keratin 5 causing epidermolysis bullosa simplex, transmitted by a mosaic sire.
Ford CA, Stanfield AM, Spelman RJ, Smits B, Ankersmidt-Udy AE, Cottier K, Holloway H, Walden A, Al-Wahb M, Bohm E, Snell RG, Sutherland GT
The Journal of investigative dermatology124(6)1170-6(2005 Jun)

Amino acid sequence diversity between bovine epidermal cytokeratin polypeptides of the basic (type II) subfamily as determined from cDNA clones.
Jorcano JL, Franz JK, Franke WW
Differentiation; research in biological diversity28(2)155-63(1984)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NMHC IIA may play critical roles in the early trophoblast-derived ectoplacental cone and extraembryonic ectoderm in Keratin 5-Cre transgenic mice.
Title: Keratin 5-Cre-driven excision of nonmuscle myosin IIA in early embryo trophectoderm leads to placenta defects and embryonic lethality.

G to A substitution in all affected animals. This mutation leads to an amino acid change of glutamic acid to lysine in the final E (478) of the KLLEGE motif of the protein.
Title: A mutation in bovine keratin 5 causing epidermolysis bullosa simplex, transmitted by a mosaic sire.

The following KRT5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa41284	NM_001008663.1 Latest version!	Bos taurus keratin 5 (KRT5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KRT5 ( NM_001008663.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001008663.1 Bos taurus keratin 5 (KRT5), mRNA.

Price & Availability↑

CloneID	OBa41284
Clone ID Related Accession (Same CDS sequence)	NM_001008663.1
Accession Version	NM_001008663.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1806bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	keratin, type II cytoskeletal 5
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY740402.1. ##Evidence-Data-START## Transcript exon combination :: BC140616.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

ATGTCTCGCC AGTCAACCGT GTCCTTCCGA AGCGGGGGCG GCCGCAGCTT CAGCACTGCC 
TCTGCCATCA CCCCGTCCGT CTCCCGCACC AGTTTCACCT CCGTGTCCCG GTCCGGGGGT 
GGCGGCGGTG GCGGCTTCGG CAGGGTCAGC CTTGGGGGTG CTTATGGTGC CGGTGGCTTT 
GGCAGCCGGA GCCTCTACAA CCTGGGGGGC TCCAAGAGGA TTTCCATTAG CGCCAGCGGC 
GGTGGATTCA GGAACCGATT TGGTGCTGGT GCTGGAGGCG GCTATGGCTT TGGAGGTGGA 
GCCGGGAGCG GATTTGGTTT TGGCGGTGGA GCTGGCGGTG GTGGCTTTGG GCTTGGTGGT 
GGAGCTGGCT TCGGAGGTGG CTTCGGTGGT CCTGGCTTCC CTGTGTGCCC CCCTGGAGGC 
ATCCAAGAGG TCACCGTCAA CCAGAGTCTC CTGACTCCCC TCAACCTGCA AATCGACCCC 
ACCATCCAGC GGGTGAGGAC TGAGGAGCGG GAGCAGATCA AGACCCTCAA CAACAAGTTT 
GCCTCTTTCA TCGACAAGGT GCGGTTCCTG GAGCAGCAGA ACAAGGTCCT GGACACCAAG 
TGGGCCCTGC TGCAGGAGCA GGGCACCAAG ACTGTGAGGC AGAACCTGGA GCCTTTGTTA 
GAGCAGTACA TCAACAACCT CAGGAGACAG CTGGATGGTA TCGTTGGGGA GAGAGGCCGC 
CTGGACTCAG AGCTCAGGAA TATGCAGGAC TTGGTGGAGG ACTTCAAGAA CAAGTATGAA 
GATGAAATCA ACAAGCGTAC CACTGCCGAG AATGAGTTTG TGATGCTGAA GAAGGATGTG 
GATGCTGCCT ACATGAACAA AGTGGAACTA GAGGCCAAGG TCGATGCACT GATGGATGAG 
ATCAACTTCA TGAAGATGTT CTTCGATGCG GAGCTGTCCC AGATGCAGAC ACACGTCTCA 
GACACATCCG TGGTCCTGTC CATGGACAAC AACCGCAGCC TGGACTTGGA CAGCATCATT 
GCTGAGGTCA AGGCCCAGTA CGAGGACATT GCCAACCGCA GCCGCACAGA AGCTGAGTCC 
TGGTACCAGA CCAAGTATGA GGAGCTGCAG CAGACTGCAG GTCGCCATGG CGATGATCTC 
CGTAACACCA AGCATGAGAT CTCTGAGATG AATAGGATGA TCCAGAGGCT GAGATCCGAG 
ATCGACAACG TTAAAAAACA GTGTGCCAAC CTGCAGAATG CCATTGCTGA CGCTGAGCAG 
CGTGGGGAGC TGGCCCTCAA GGATGCCAGG AGCAAGCTGG CGGAGCTGGA GGACGCCCTG 
CAGAAGGCCA AGCAGGACAT GGCACGTCTG CTGAGGGAGT ACCAGGAGCT GATGAACACC 
AAGCTGGCCC TGGACGTGGA GATCGCCACC TACAGGAAGC TGCTGGAGGG CGAGGAGTGC 
AGACTGAGTG GAGAAGGAGT TGGACCAGTC AACATCTCTG TCGTCACAAA CACCGTTTCC 
TCAGGCTACG GTGGTGGCAG TGGCTTTGGC GGTGGCCTGG GCGGTGGCCT GGGCGGTGGC 
CTGGGTGGCG GCCTCGGCGG CGGCCTGGGC GGAGGTCTGG GCAGTGGTCT TGGTGGAGGC 
GGCAGTAGCA GCTTCTACTC CAGCAGCAGT GGGGGTGTTG GCCTAGGCGG TGGGCTCAGT 
GTCGGGGGCT CTGGCTTCAG TGCAAGCAGT GGCCGAAGCT TGGGCTTTGG CAGTGGTGGG 
GGCAGCAGCT CCAGCGTCAA ATTTGTCTCC ACCACCTCCT CCTCTCGGAA GAGCTTCAAG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008663.1
CDS	1..1806
Translation	MSRQSTVSFRSGGGRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLGGAYGAGGFGSRSLYNLGGSKRISISASGGGFRNRFGAGAGGGYGFGGGAGSGFGFGGGAGGGGFGLGGGAGFGGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPTIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWALLQEQGTKTVRQNLEPLLEQYINNLRRQLDGIVGERGRLDSELRNMQDLVEDFKNKYEDEINKRTTAENEFVMLKKDVDAAYMNKVELEAKVDALMDEINFMKMFFDAELSQMQTHVSDTSVVLSMDNNRSLDLDSIIAEVKAQYEDIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRSEIDNVKKQCANLQNAIADAEQRGELALKDARSKLAELEDALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNISVVTNTVSSGYGGGSGFGGGLGGGLGGGLGGGLGGGLGGGLGSGLGGGGSSSFYSSSSGGVGLGGGLSVGGSGFSASSGRSLGFGSGGGSSSSVKFVSTTSSSRKSFKS

Target ORF information:

RefSeq Version	NM_001008663.1
Organism	Bos taurus(cattle)
Definition	Bos taurus keratin 5 (KRT5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008663.1

ORF Insert Sequence:

ATGTCTCGCC AGTCAACCGT GTCCTTCCGA AGCGGGGGCG GCCGCAGCTT CAGCACTGCC 
TCTGCCATCA CCCCGTCCGT CTCCCGCACC AGTTTCACCT CCGTGTCCCG GTCCGGGGGT 
GGCGGCGGTG GCGGCTTCGG CAGGGTCAGC CTTGGGGGTG CTTATGGTGC CGGTGGCTTT 
GGCAGCCGGA GCCTCTACAA CCTGGGGGGC TCCAAGAGGA TTTCCATTAG CGCCAGCGGC 
GGTGGATTCA GGAACCGATT TGGTGCTGGT GCTGGAGGCG GCTATGGCTT TGGAGGTGGA 
GCCGGGAGCG GATTTGGTTT TGGCGGTGGA GCTGGCGGTG GTGGCTTTGG GCTTGGTGGT 
GGAGCTGGCT TCGGAGGTGG CTTCGGTGGT CCTGGCTTCC CTGTGTGCCC CCCTGGAGGC 
ATCCAAGAGG TCACCGTCAA CCAGAGTCTC CTGACTCCCC TCAACCTGCA AATCGACCCC 
ACCATCCAGC GGGTGAGGAC TGAGGAGCGG GAGCAGATCA AGACCCTCAA CAACAAGTTT 
GCCTCTTTCA TCGACAAGGT GCGGTTCCTG GAGCAGCAGA ACAAGGTCCT GGACACCAAG 
TGGGCCCTGC TGCAGGAGCA GGGCACCAAG ACTGTGAGGC AGAACCTGGA GCCTTTGTTA 
GAGCAGTACA TCAACAACCT CAGGAGACAG CTGGATGGTA TCGTTGGGGA GAGAGGCCGC 
CTGGACTCAG AGCTCAGGAA TATGCAGGAC TTGGTGGAGG ACTTCAAGAA CAAGTATGAA 
GATGAAATCA ACAAGCGTAC CACTGCCGAG AATGAGTTTG TGATGCTGAA GAAGGATGTG 
GATGCTGCCT ACATGAACAA AGTGGAACTA GAGGCCAAGG TCGATGCACT GATGGATGAG 
ATCAACTTCA TGAAGATGTT CTTCGATGCG GAGCTGTCCC AGATGCAGAC ACACGTCTCA 
GACACATCCG TGGTCCTGTC CATGGACAAC AACCGCAGCC TGGACTTGGA CAGCATCATT 
GCTGAGGTCA AGGCCCAGTA CGAGGACATT GCCAACCGCA GCCGCACAGA AGCTGAGTCC 
TGGTACCAGA CCAAGTATGA GGAGCTGCAG CAGACTGCAG GTCGCCATGG CGATGATCTC 
CGTAACACCA AGCATGAGAT CTCTGAGATG AATAGGATGA TCCAGAGGCT GAGATCCGAG 
ATCGACAACG TTAAAAAACA GTGTGCCAAC CTGCAGAATG CCATTGCTGA CGCTGAGCAG 
CGTGGGGAGC TGGCCCTCAA GGATGCCAGG AGCAAGCTGG CGGAGCTGGA GGACGCCCTG 
CAGAAGGCCA AGCAGGACAT GGCACGTCTG CTGAGGGAGT ACCAGGAGCT GATGAACACC 
AAGCTGGCCC TGGACGTGGA GATCGCCACC TACAGGAAGC TGCTGGAGGG CGAGGAGTGC 
AGACTGAGTG GAGAAGGAGT TGGACCAGTC AACATCTCTG TCGTCACAAA CACCGTTTCC 
TCAGGCTACG GTGGTGGCAG TGGCTTTGGC GGTGGCCTGG GCGGTGGCCT GGGCGGTGGC 
CTGGGTGGCG GCCTCGGCGG CGGCCTGGGC GGAGGTCTGG GCAGTGGTCT TGGTGGAGGC 
GGCAGTAGCA GCTTCTACTC CAGCAGCAGT GGGGGTGTTG GCCTAGGCGG TGGGCTCAGT 
GTCGGGGGCT CTGGCTTCAG TGCAAGCAGT GGCCGAAGCT TGGGCTTTGG CAGTGGTGGG 
GGCAGCAGCT CCAGCGTCAA ATTTGTCTCC ACCACCTCCT CCTCTCGGAA GAGCTTCAAG 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of Intrauterine Growth Restriction During Late Pregnancy on the Development of the Ovine Fetal Thymus and the T-Lymphocyte Subpopulation.
American journal of reproductive immunology (New York, N.Y. : 1989)74(1)26-37(2015 Jul)
Liu Y,He S,Zhang Y,Xia W,Li M,Zhang C,Gao F

Keratin 5-Cre-driven excision of nonmuscle myosin IIA in early embryo trophectoderm leads to placenta defects and embryonic lethality.
Developmental biology382(1)136-48(2013 Oct)
Crish J,Conti MA,Sakai T,Adelstein RS,Egelhoff TT

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

A mutation in bovine keratin 5 causing epidermolysis bullosa simplex, transmitted by a mosaic sire.
The Journal of investigative dermatology124(6)1170-6(2005 Jun)
Ford CA,Stanfield AM,Spelman RJ,Smits B,Ankersmidt-Udy AE,Cottier K,Holloway H,Walden A,Al-Wahb M,Bohm E,Snell RG,Sutherland GT

Amino acid sequence diversity between bovine epidermal cytokeratin polypeptides of the basic (type II) subfamily as determined from cDNA clones.
Differentiation; research in biological diversity28(2)155-63(1984)
Jorcano JL,Franz JK,Franke WW