MDK cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MDK
Entrez Gene ID	280852
Full Name	midkine
General protein information	Preferred Names midkine Names midkine midkine (neurite growth-promoting factor 2)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005216403.3	XP_005216460.1	midkine isoform X2
XM_005216404.3	XP_005216461.1	midkine isoform X2
XM_005216404.4	XP_005216461.1	midkine isoform X2
XM_005216402.3	XP_005216459.1	midkine isoform X1
NM_173935.3	NP_776360.2	midkine precursor
XM_005216402.4	XP_005216459.1	midkine isoform X1
XM_005216403.4	XP_005216460.1	midkine isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MDK	NP_002382.1
Danio rerio (zebrafish)	mdka	NP_571145.1
Danio rerio (zebrafish)	mdkb	NP_571791.1
Rattus norvegicus (Norway rat)	Mdk	NP_110486.1
	MDK	XP_002799613.1
Mus musculus (house mouse)	Mdk	NP_034914.1
Gallus gallus (chicken)	MDK	NP_001106760.1
Bos taurus (cattle)	MDK	NP_776360.2
Xenopus tropicalis (tropical clawed frog)	mdk	NP_989074.1

Gene Ontology
Bibliography

Related articles in PubMed

9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells.
G?mez E, Royo LJ, Duque P, Carneiro G, Hidalgo C, Goyache F, Lorenzo PL, Alvarez I, Facal N, D?ez C
Molecular reproduction and development66(3)247-55(2003 Nov)

cDNA cloning of bovine midkine and production of the recombinant protein, which affects in vitro maturation of bovine oocytes.
Ikeda S, Nishikimi A, Ichihara-Tanaka K, Muramatsu T, Yamada M
Molecular reproduction and development57(1)99-107(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that 9-cis-retinoic acid enhanced midkine gene expression, but not insulin-like growth factor-I gene expression, in cumulus-granulosa cells.
Title: 9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells.

The following MDK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MDK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39108	NM_173935.3 Latest version!	Bos taurus midkine (MDK), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa26706	XM_005216402.4 Latest version!	Bos taurus midkine (MDK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa26707	XM_005216403.4 Latest version!	Bos taurus midkine (MDK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa26707	XM_005216404.4 Latest version!	Bos taurus midkine (MDK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
View Old Accession Versions >>
OBa26707	XM_005216404.3	Bos taurus midkine (neurite growth-promoting factor 2) (MDK), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa26706	XM_005216402.3	Bos taurus midkine (neurite growth-promoting factor 2) (MDK), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
OBa26707	XM_005216403.3	Bos taurus midkine (neurite growth-promoting factor 2) (MDK), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MDK ( NM_173935.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776360.2 Bos taurus midkine (MDK), mRNA.

Price & Availability↑

CloneID	OBa39108
Clone ID Related Accession (Same CDS sequence)	NM_173935.3
Accession Version	NM_173935.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-13
Organism	Bos taurus(cattle)
Product	midkine precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC103211.1. On Aug 31, 2012 this sequence version replaced NM_173935.2. ##Evidence-Data-START## Transcript exon combination :: BC103211.1, DV918928.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTACAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776360.2
CDS	49..480
Translation	MQRRSFLLLALLALLALTSAVAKKKDKMKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFETWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPYSPKTKAKAKAKKGKEKD

Target ORF information:

RefSeq Version	NM_173935.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midkine (MDK), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173935.3

ORF Insert Sequence:

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTACAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005216402.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216459.1 Bos taurus midkine (neurite growth-promoting factor 2) (MDK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26706
Clone ID Related Accession (Same CDS sequence)	XM_005216402.3 , XM_005216402.4
Accession Version	XM_005216402.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midkine isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216402.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAGG ACCCGGCTCA CAAGAGGATG CAGCGCCGAA GCTTCCTCCT CCTCGCCCTT 
CTCGCCTTGC TGGCGCTCAC CTCCGCGGTG GCCAAAAAGA AAGACAAAAT GAAGAAGGGA 
GGCCCAGGGA GCGAGTGTGC CGAGTGGACC TGGGGGCCCT GCACCCCCAG CAGCAAGGAC 
TGCGGCGTGG GATTCCGCGA GGGCACCTGC GGGGCCCAGA CGCAGCGCAT CCGGTGCCGG 
GTGCCCTGTA ACTGGAAGAA GGAGTTTGGA GCCGACTGCA AGTACAAGTT TGAGACCTGG 
GGGGCGTGTG ATGGGGGCAC AGGCACCAAA GCCCGCCAAG GGACCCTGAA GAAGGCGCGA 
TACAATGCCC AGTGCCAGGA GACCATCCGA GTGACCAAGC CCTGCAGCCC CAAGACCAAA 
GCCAAGGCCA AAGCTAAGAA AGGGAAGGAA AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216459.1
CDS	184..642
Translation	MPEDPAHKRMQRRSFLLLALLALLALTSAVAKKKDKMKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFETWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCSPKTKAKAKAKKGKEKD

Target ORF information:

RefSeq Version	XM_005216402.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midkine (neurite growth-promoting factor 2) (MDK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216402.3

ORF Insert Sequence:

ATGCCCGAGG ACCCGGCTCA CAAGAGGATG CAGCGCCGAA GCTTCCTCCT CCTCGCCCTT 
CTCGCCTTGC TGGCGCTCAC CTCCGCGGTG GCCAAAAAGA AAGACAAAAT GAAGAAGGGA 
GGCCCAGGGA GCGAGTGTGC CGAGTGGACC TGGGGGCCCT GCACCCCCAG CAGCAAGGAC 
TGCGGCGTGG GATTCCGCGA GGGCACCTGC GGGGCCCAGA CGCAGCGCAT CCGGTGCCGG 
GTGCCCTGTA ACTGGAAGAA GGAGTTTGGA GCCGACTGCA AGTACAAGTT TGAGACCTGG 
GGGGCGTGTG ATGGGGGCAC AGGCACCAAA GCCCGCCAAG GGACCCTGAA GAAGGCGCGA 
TACAATGCCC AGTGCCAGGA GACCATCCGA GTGACCAAGC CCTGCAGCCC CAAGACCAAA 
GCCAAGGCCA AAGCTAAGAA AGGGAAGGAA AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005216402.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216459.1 Bos taurus midkine (MDK), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26706
Clone ID Related Accession (Same CDS sequence)	XM_005216402.3 , XM_005216402.4
Accession Version	XM_005216402.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	midkine isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216402.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCGAGG ACCCGGCTCA CAAGAGGATG CAGCGCCGAA GCTTCCTCCT CCTCGCCCTT 
CTCGCCTTGC TGGCGCTCAC CTCCGCGGTG GCCAAAAAGA AAGACAAAAT GAAGAAGGGA 
GGCCCAGGGA GCGAGTGTGC CGAGTGGACC TGGGGGCCCT GCACCCCCAG CAGCAAGGAC 
TGCGGCGTGG GATTCCGCGA GGGCACCTGC GGGGCCCAGA CGCAGCGCAT CCGGTGCCGG 
GTGCCCTGTA ACTGGAAGAA GGAGTTTGGA GCCGACTGCA AGTACAAGTT TGAGACCTGG 
GGGGCGTGTG ATGGGGGCAC AGGCACCAAA GCCCGCCAAG GGACCCTGAA GAAGGCGCGA 
TACAATGCCC AGTGCCAGGA GACCATCCGA GTGACCAAGC CCTGCAGCCC CAAGACCAAA 
GCCAAGGCCA AAGCTAAGAA AGGGAAGGAA AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216459.1
CDS	176..634
Translation	MPEDPAHKRMQRRSFLLLALLALLALTSAVAKKKDKMKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFETWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCSPKTKAKAKAKKGKEKD

Target ORF information:

RefSeq Version	XM_005216402.4
Organism	Bos taurus(cattle)
Definition	Bos taurus midkine (MDK), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216402.4

ORF Insert Sequence:

ATGCCCGAGG ACCCGGCTCA CAAGAGGATG CAGCGCCGAA GCTTCCTCCT CCTCGCCCTT 
CTCGCCTTGC TGGCGCTCAC CTCCGCGGTG GCCAAAAAGA AAGACAAAAT GAAGAAGGGA 
GGCCCAGGGA GCGAGTGTGC CGAGTGGACC TGGGGGCCCT GCACCCCCAG CAGCAAGGAC 
TGCGGCGTGG GATTCCGCGA GGGCACCTGC GGGGCCCAGA CGCAGCGCAT CCGGTGCCGG 
GTGCCCTGTA ACTGGAAGAA GGAGTTTGGA GCCGACTGCA AGTACAAGTT TGAGACCTGG 
GGGGCGTGTG ATGGGGGCAC AGGCACCAAA GCCCGCCAAG GGACCCTGAA GAAGGCGCGA 
TACAATGCCC AGTGCCAGGA GACCATCCGA GTGACCAAGC CCTGCAGCCC CAAGACCAAA 
GCCAAGGCCA AAGCTAAGAA AGGGAAGGAA AAGGACTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005216403.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216460.1 Bos taurus midkine (neurite growth-promoting factor 2) (MDK), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa26707
Clone ID Related Accession (Same CDS sequence)	XM_005216403.3 , XM_005216404.3 , XM_005216404.4 , XM_005216403.4
Accession Version	XM_005216403.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midkine isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216403.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTGCAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216460.1
CDS	400..831
Translation	MQRRSFLLLALLALLALTSAVAKKKDKMKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFETWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCSPKTKAKAKAKKGKEKD

Target ORF information:

RefSeq Version	XM_005216403.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midkine (neurite growth-promoting factor 2) (MDK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216403.3

ORF Insert Sequence:

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTGCAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005216404.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216461.1 Bos taurus midkine (neurite growth-promoting factor 2) (MDK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa26707
Clone ID Related Accession (Same CDS sequence)	XM_005216403.3 , XM_005216404.3 , XM_005216404.4 , XM_005216403.4
Accession Version	XM_005216404.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	midkine isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216404.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTGCAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216461.1
CDS	227..658
Translation	MQRRSFLLLALLALLALTSAVAKKKDKMKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFETWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCSPKTKAKAKAKKGKEKD

Target ORF information:

RefSeq Version	XM_005216404.3
Organism	Bos taurus(cattle)
Definition	Bos taurus midkine (neurite growth-promoting factor 2) (MDK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216404.3

ORF Insert Sequence:

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTGCAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005216404.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216461.1 Bos taurus midkine (MDK), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa26707
Clone ID Related Accession (Same CDS sequence)	XM_005216403.3 , XM_005216404.3 , XM_005216404.4 , XM_005216403.4
Accession Version	XM_005216404.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	midkine isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216404.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTGCAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216461.1
CDS	214..645
Translation	MQRRSFLLLALLALLALTSAVAKKKDKMKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFETWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCSPKTKAKAKAKKGKEKD

Target ORF information:

RefSeq Version	XM_005216404.4
Organism	Bos taurus(cattle)
Definition	Bos taurus midkine (MDK), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216404.4

ORF Insert Sequence:

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTGCAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDK ( XM_005216403.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216460.1 Bos taurus midkine (MDK), transcript variant X2, mRNA.

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CloneID	OBa26707
Clone ID Related Accession (Same CDS sequence)	XM_005216403.3 , XM_005216404.3 , XM_005216404.4 , XM_005216403.4
Accession Version	XM_005216403.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 432bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	midkine isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216403.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTGCAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216460.1
CDS	386..817
Translation	MQRRSFLLLALLALLALTSAVAKKKDKMKKGGPGSECAEWTWGPCTPSSKDCGVGFREGTCGAQTQRIRCRVPCNWKKEFGADCKYKFETWGACDGGTGTKARQGTLKKARYNAQCQETIRVTKPCSPKTKAKAKAKKGKEKD

Target ORF information:

RefSeq Version	XM_005216403.4
Organism	Bos taurus(cattle)
Definition	Bos taurus midkine (MDK), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216403.4

ORF Insert Sequence:

ATGCAGCGCC GAAGCTTCCT CCTCCTCGCC CTTCTCGCCT TGCTGGCGCT CACCTCCGCG 
GTGGCCAAAA AGAAAGACAA AATGAAGAAG GGAGGCCCAG GGAGCGAGTG TGCCGAGTGG 
ACCTGGGGGC CCTGCACCCC CAGCAGCAAG GACTGCGGCG TGGGATTCCG CGAGGGCACC 
TGCGGGGCCC AGACGCAGCG CATCCGGTGC CGGGTGCCCT GTAACTGGAA GAAGGAGTTT 
GGAGCCGACT GCAAGTACAA GTTTGAGACC TGGGGGGCGT GTGATGGGGG CACAGGCACC 
AAAGCCCGCC AAGGGACCCT GAAGAAGGCG CGATACAATG CCCAGTGCCA GGAGACCATC 
CGAGTGACCA AGCCCTGCAG CCCCAAGACC AAAGCCAAGG CCAAAGCTAA GAAAGGGAAG 
GAAAAGGACT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

9-cis-retinoic acid during in vitro maturation improves development of the bovine oocyte and increases midkine but not IGF-I expression in cumulus-granulosa cells.
Molecular reproduction and development66(3)247-55(2003 Nov)
G?mez E,Royo LJ,Duque P,Carneiro G,Hidalgo C,Goyache F,Lorenzo PL,Alvarez I,Facal N,D?ez C

cDNA cloning of bovine midkine and production of the recombinant protein, which affects in vitro maturation of bovine oocytes.
Molecular reproduction and development57(1)99-107(2000 Sep)
Ikeda S,Nishikimi A,Ichihara-Tanaka K,Muramatsu T,Yamada M

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MDK cDNA ORF clone, Bos taurus(cattle)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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