GCLC cDNA ORF clone, Bos mutus(wild yak)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCLC
Entrez Gene ID	102268521
Full Name	glutamate-cysteine ligase catalytic subunit
Gene Type	protein-coding
Organism	Bos mutus(wild yak)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014476873.1	XP_014332359.1	glutamate--cysteine ligase catalytic subunit

Pathways from BioSystems
Gene Ontology

The following GCLC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCLC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo04929	XM_014476873.1 Latest version!	Bos mutus glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCLC ( XM_014476873.1 ) cDNA ORF clone, Bos mutus(wild yak) -> XP_014332359.1 Bos mutus glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.

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CloneID	OBo04929
Clone ID Related Accession (Same CDS sequence)	XM_014476873.1
Accession Version	XM_014476873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2514bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Bos mutus(wild yak)
Product	glutamate--cysteine ligase catalytic subunit
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005393281.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos mutus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 29% of CDS bases ##RefSeq-Attributes-END##

ATGCGCTCCG TCCACCCGCT TCCGGGCTCA CAGGTCTTGC CCTCAGCCCC CTCCTTAGAT 
CTTGCTTCTT CCCTTCTGAA GGACAAGCGC AGACAGCTCT TACAGCCTTT TCTCAATTCG 
GGGCTCTTAT GGGTCTCCAG CATACATTTA ATGGGTTCTG TGTCCCTCTC CACGGCTCTT 
CTGTGCCTGC AGCCCCGGGA CTCCGGCTCT CGCAGGACTC ACACCTCCAC ACCAGCTGCC 
GACCCTGGTC AGCGCAGGTC CATCCGCCGT TTCAAGCGCG GCTGTTCGGA TCTTGCCGCA 
CTGGTCTCCG CTGTTTCACT GCAGGTGATT CTGAAGCCAA GTAGCATCAC TGTGGCAGGC 
ACGTCCTTGG GTGCTCCAGG CTTGGGTGTC ATGCTCGATG TGAAAATGGG TGCCATCCAG 
CTGGATGGTC AAAAGTGCTC AAAAAACGCC CCGCGTGAGC TGAGAGCAGG AGTCATCCGC 
CTGGCTCTGC ACCGGCATTG CTCCCAGCAC TTGGGGAGGA TAGGACTTGA GTCAAAGGAC 
TTGCAGGGGG TGCTGCGGGA CACTGCGTTT CGGCACTCGA GGGTCATCGG CCACTTAGAG 
CCTGACCACA GGATGGCTTC GGGCTTGTTC AGGAGATCAG TAAGGGACTA CGTCAAACAG 
GAGAAGACAG ACTTGAGTGA GAGCTCTGGT CCTCCTGCCA GACCACTGTT GGCCGCTCTC 
TCCGGGTGTC GCGTCATACA GCACCATCAT GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCG ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACGTCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ATGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TTGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAATGCCGT GGTGGACGGC 
TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC 
GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC 
TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG 
CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT 
ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC 
CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA 
CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCGC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014332359.1
CDS	1..2514
Translation	MRSVHPLPGSQVLPSAPSLDLASSLLKDKRRQLLQPFLNSGLLWVSSIHLMGSVSLSTALLCLQPRDSGSRRTHTSTPAADPGQRRSIRRFKRGCSDLAALVSAVSLQVILKPSSITVAGTSLGAPGLGVMLDVKMGAIQLDGQKCSKNAPRELRAGVIRLALHRHCSQHLGRIGLESKDLQGVLRDTAFRHSRVIGHLEPDHRMASGLFRRSVRDYVKQEKTDLSESSGPPARPLLAALSGCRVIQHHHVEYMLVSFDHEKKKAQLVLSGEELLETLQEKGERTNPNHPTLWRPEYGSYMIEGTPGQPYGGTMSEFNTVEDNMRKRRKEATSLLGENQALCTITSFPRLGCPGFTLPEFKPNPVEGGASKSLFFPDEAINKHPRFSTLTRNIRHRRGEKVVINVPIFKDKNTPAPFIETFPEDEEATKASKPDHIYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRGYVSDIDCRWGVISASVDDRTREERGLEPLKNNHYRISKSRYDSIDSYLSECGEKYNDIDLTKDKEIYEQLLQEGLDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPPPNSDIGWRVEFRPMEVQLTDFENSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQELFYFRKDICKGGNAVVDGCGKAAGRAEPAAEEYALMTIDTIINGKEGVFPGLIPVLNSYLESMEVDVDTRCSILNYLKLIKKRASGELMTVAKWMREFIAKHPDYKQDSVITDQMNYSLLLKCNQIANESCECPELLGPAFRKVRYSGSKTDSSA

Target ORF information:

RefSeq Version	XM_014476873.1
Organism	Bos mutus(wild yak)
Definition	Bos mutus glutamate-cysteine ligase, catalytic subunit (GCLC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014476873.1

ORF Insert Sequence:

ATGCGCTCCG TCCACCCGCT TCCGGGCTCA CAGGTCTTGC CCTCAGCCCC CTCCTTAGAT 
CTTGCTTCTT CCCTTCTGAA GGACAAGCGC AGACAGCTCT TACAGCCTTT TCTCAATTCG 
GGGCTCTTAT GGGTCTCCAG CATACATTTA ATGGGTTCTG TGTCCCTCTC CACGGCTCTT 
CTGTGCCTGC AGCCCCGGGA CTCCGGCTCT CGCAGGACTC ACACCTCCAC ACCAGCTGCC 
GACCCTGGTC AGCGCAGGTC CATCCGCCGT TTCAAGCGCG GCTGTTCGGA TCTTGCCGCA 
CTGGTCTCCG CTGTTTCACT GCAGGTGATT CTGAAGCCAA GTAGCATCAC TGTGGCAGGC 
ACGTCCTTGG GTGCTCCAGG CTTGGGTGTC ATGCTCGATG TGAAAATGGG TGCCATCCAG 
CTGGATGGTC AAAAGTGCTC AAAAAACGCC CCGCGTGAGC TGAGAGCAGG AGTCATCCGC 
CTGGCTCTGC ACCGGCATTG CTCCCAGCAC TTGGGGAGGA TAGGACTTGA GTCAAAGGAC 
TTGCAGGGGG TGCTGCGGGA CACTGCGTTT CGGCACTCGA GGGTCATCGG CCACTTAGAG 
CCTGACCACA GGATGGCTTC GGGCTTGTTC AGGAGATCAG TAAGGGACTA CGTCAAACAG 
GAGAAGACAG ACTTGAGTGA GAGCTCTGGT CCTCCTGCCA GACCACTGTT GGCCGCTCTC 
TCCGGGTGTC GCGTCATACA GCACCATCAT GTGGAGTACA TGTTGGTGTC TTTTGATCAT 
GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA 
AAGGGGGAAA GGACGAACCC AAACCATCCG ACCCTTTGGA GACCAGAATA TGGGAGCTAC 
ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT 
GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACGTCTC TCCTGGGGGA AAACCAGGCC 
CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC 
AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC 
AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA 
GTGGTCATCA ATGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT 
TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC 
ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT 
GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT 
GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT 
TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC 
CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT 
GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG 
GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG 
ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT 
ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT 
GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG 
TATGTGGTGT TTGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC 
ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG 
CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAATGCCGT GGTGGACGGC 
TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC 
GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC 
TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG 
CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT 
ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC 
CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA 
CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCGC TTAG