GCN1 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	GCN1
Entrez Gene ID	109571175
Full Name	GCN1, eIF2 alpha kinase activator homolog
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019977433.1	XP_019832992.1	eIF-2-alpha kinase activator GCN1

Pathways from BioSystems
Gene Ontology

The following GCN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo34370	XM_019977433.1 Latest version!	Bos indicus GCN1, eIF2 alpha kinase activator homolog (GCN1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GCN1 ( XM_019977433.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019832992.1 Bos indicus GCN1, eIF2 alpha kinase activator homolog (GCN1), mRNA.

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CloneID	OBo34370
Clone ID Related Accession (Same CDS sequence)	XM_019977433.1
Accession Version	XM_019977433.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	eIF-2-alpha kinase activator GCN1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032666.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA 
GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA 
TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC 
CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC 
AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT 
GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC 
AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC 
GCCGTGGACA GTGCYGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG 
GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC GGGCATGCTG 
GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG 
AGTGCYCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG 
TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT 
CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT 
ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG 
AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG 
GCRCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG 
CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG 
AGCGTTCTCT CAGGGATCGG RAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA 
GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA 
GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA 
GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT 
GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG 
CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC 
ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA 
ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG 
AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT 
ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC CGCACCGACT CACAGGCAAC 
AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG 
CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG 
GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC 
GAGGCTCTGG TGACCGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG 
CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC 
ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG 
GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC 
TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC 
CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG 
CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG 
GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC 
TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAARAA GAAAGGCATA 
AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG 
GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG 
CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG 
GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT 
TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG 
AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG 
GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC 
AGCAGGGCAG GCAAGGGCGA GCCAGAYGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG 
GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG 
GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA 
AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG 
CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA 
GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG 
CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC 
ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG 
AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG 
ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC 
TGCTCCTTGC TCATCGAYGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG 
GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG 
CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA 
CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC 
CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT 
GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT 
CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG 
GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG 
GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT 
GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC 
AGCTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG 
CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG 
CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC 
GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT 
GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG 
CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT 
GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC 
TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG 
CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATCGTG 
CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCARAAGGC TGGGCAGCAG 
GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA 
CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG 
GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC 
CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT 
GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT 
CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG 
ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG 
GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG 
GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC 
AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC 
TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA 
GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG 
GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC 
GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG 
GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT 
CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG 
CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT 
GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT 
AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC 
CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG 
CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG 
TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG 
GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC 
CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT 
GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC 
GTGCCCAAGC TGACGACCCC GCCCGTCAAC ACCCGGGTGC TGGCTTTCCT TTCATCTGTG 
GCGGGTGATG CCCTGACACG TCATCTTGGC GTGATCCTCC CGGCGGTCAT GTTGGCCCTG 
AAGGAGAAGC TGGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCCGTGATC 
CTGTCCGTGG AGGACGACAC CGGGCACCGG ATCATCATCG AGGACCTGTT GGAAGCCACC 
CGCAGCCCTG AGGTGGGCAT GAGGCAGGCC GCGGCCATCA TCCTCAACAT CTACTGCTCA 
CGCTCCAAGG CGGACTACAC TAGCCACCTG CGGAGCCTGG TCTCAGGCCT GATCCGCCTC 
TTCAACGACT CCAGCCCCGT GGTTCTGGAG GAGAGCTGGG ACGCCCTGAA TGCCATCACC 
AAGAAACTGG ATGCAGGCAA CCAGCTGGCC CTCATTGAGG AACTGCACAA GGAAATCCGG 
TTCATAGGAA ATGAGAGCAG AGGCGAGCAC GTGCCAGGCT TCTGCCTCCC GAAGAAGGGG 
GTAACGTCCA TCCTTCCAGT GCTGCGGGAG GGGGTCCTGA CTGGCAGCCC GGAACAGAAG 
GAGGAAGCTG CCAAAGCGTT AGGCTTGGTG ATCCGCCTGA CCTCGGCGGA CGCCCTGMGG 
CCATCCGTGG TCAACATCAC TGGCCCTCTG ATTCGCATCC TGGGGGACCG GTTCAGCTGG 
AACGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGC TGGCCAAGGT CGGGATCGCC 
CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACCTTCACCA AAGCCCTGCA GGACTCTAAC 
CGCGGCGTGC GCCTGAAGGC CGCCGATGCG CTAGGAAAGC TCATTTCCAT CCACATCAAG 
GTGGATCCTC TTTTCACGGA GCTGCTGAAT GGCATCCGGG TCATGGAGGA CCCAGGCRTC 
AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCGGGGGC CAAAGTGGAT 
GTGGTCATCC GGAAAAACAT CGTCTCGCTC CTGCTGAGCA TGCTGGGACA CGACGAGGAC 
AACACGCGCA TCTCCTCRGC CGGGTGCCTG GGGGAGCTAT GTGCCTTTCT GACGGAAGAG 
GAGCTCAGCA CTGTTCTTCA GCAGTACTTG CTGGCGGATG TGTCTGGCAT TGACTGGATG 
GTTCGGCATG GGCGGAGCCT GGCTCTCTCC GTGGCTGTGA GCGTGGCTCC TGGCAGACTC 
TGCGTAGGCA AGTATGGCAG CGACGTCCAG GACATGATCC TCAGCAATGC CATGGCAGAC 
AGGATCCCCA TCGCCGTGAG TGGGGTCCGG GGCATGGGCT TCCTGATGAA GCACCACATC 
GAGACCGGAG GAGGACAGTT GCCGGCCAAA CTCTCCAGCC TGTTCATTAA GTGTCTGCAG 
AACCCATCCA GTGACATCAG GTTGGTGGCT GAGAAGATGA TCTGGTGGGC AAACAAGGAC 
CCGCTGCCTG CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCCCT CCTGGACAAC 
ACCAAGGATA AAAACACCGT CGTCAGGGCC TACAGCGACC AGGCGATTGT CAACCTCCTC 
AAGATGAGGC AGGGAGAGGA GGTGTTTCAG TCCCTCTGCA AGATCCTGGA TGTGGCCAGC 
TTGGAAGTGC TGAATGAATG CAACCGGAGG TCTCTGAAGA AGCTGGCCGG CCAGGCTGAC 
TCCACAGAGC AGGTGGACGA CACCATCCTG ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019832992.1
CDS	24..8039
Translation	MAADTQVSETLKRFAGKVTTASVKERREILSELGKCVAGKDLPEGAVKGLCKLFCLTLHRYRDAASRRALQAAIQQLAEAQPEATAKNLLHSLQSSGIGSKAGVPSKSSGSAALLALSWTGLLVRIVFPSRAKRQGDIWNKLVEVQCLLLLEVLGGSHKHAVDSnullVKKLTKLWRENPGLVEQYLSAILSLEPNQSYAGMLGLLVQFCTSHKEMDVANQHKSnullLLDFYVKNILMSKVKPQKYLLDNCAPLLRYVSHSEFKDLVLPTIQKSLLRSPENVIETISSLLASVTLDLSQYALDIGKGLAGQLKSNSPRLMDEAVLnullLRNLARQCSDSSAMEALARHLFAILGGSEGKLTIVAQKMSVLSGInullSVSHHVVSGPSSQVLSGTVAELFIPFLQQEVHEGTLVHAVSVLALWCHRFTTEVPKKLTEWFKKAFSLKTSTSAVRHAYLQCMLVCFRGDTLLQALDLLPLLIQTVEKAASQGTQVPVVTEGVAAALLLTKLSMADSQAEAKLSGFWQLILDEKKQVFTSEKFLLMASEDALCTVLHLTERLFLDHPHRLTGNKVQQYHRALAAVLLSRPWHVRRQAQQTARKLLSSLGGFKLAYGLLEELKTVLSSHKVLPFEALVTDTGEVTEAGKAYVPPRVLQEALCVISGVPGLEGGITGMEQLAQEMLIISHHPSLVAVQSGLWPALLARMKIDPEAFISRHLDQIIPRITTQNPLNQSSMNAMGSLSILSPDRVLPQLISTITTSVQNPALCQVTREEFAIMQTPAGELYDKSIIQSAQQDSIKKANMKRENKAYSFKEQIIELELKEEIKnullKKGIKEEVQLTSKQKEMLQAQLDKEAQIRRRLQELDSELEAALGLLDTILAKNPSGLTQYIPVLVDSFLPLLKSPLAAPRIKTPFLSLAACVMPPRLKALGTLVSHVTLRLLKPECALDESWCQEELSVAVKRAVTLLHSHTITSRAGKGEPnullAAPLSAPAFSLVFPFLKMVLTEMPHHSEEEEERMAQILQILTVHAQLRASPSNPPGRVDENGPELLPRVAMLRLLTWVIGTGSPRLQVLASEALTTLCASSSGEDGCAFAEQEEVDVLLCALLSPCANVRDTVLRGLLELHMVLPAPDTDEKNGLNLLRRLWVVKFDKEEEIRKLAERLWSTMGLDLQPDLCSLLInullDVIYHEAAVRQAGAEALSQAVARYQRQAAEVMGRLMEIYQEKLYRPPPVLDALGRVISESPPDQWEARCGLALALNKLSQCLDSSQVKPLFQFFVPDALNDRNPDVRKCMLDAALATLNTHGKENVNSLLPVFEEFLKNAPNDASYDAVRQSVVVLMGSLAKHLDKSDPKVKPIVAKLIAALATPSQQVQESVASCLPPLVPAIKEDAGGMIQRLMQQLLESDKYAERKGAAYGLAGLVKGLGILSLKQQEMMAALTDAIQDKKNFRRREGALFAFEMLCTMLGKLFEPYVVHVLPHLLLCFGDGNQYVREAADDCAKAVMSNLSAHGVKLVLPSLLAALEEESWRTKAGSVELLGAMAYCAPKQLSSCLPNIVPKLTEVLTDSHVKVnullKAGQQALRQIGSVIRNPEILAIAPVLLDALTDPSRKTQKCLQTLLDTKFVHFIDAPSLALIMPIVQRAFQDRSTDTRKMAAQIIGNMYSLTDQKDLAPYLPSVTPGLKASLLDPVPEVRTVSAKALGAMVKGMGESCFEDLLPWLMETLTYEQSSVDRSGAAQGLAEVMAGLGVEKLEKLMPEIVATASKVDIAPHVRDGYIMMFNYLPITFGDKFTPYVGPIIPCILKALADENEFVRDTALRAGQRVISMYAETAIALLLPQLEQGLFDDLWRIRFSSVQLLGDLLFHISGVTGKMTTETASEDDNFGTAQSNKAIITALGVDRRNRVLAGLYMGRSDTQLVVRQASLHVWKIVVSNTPRTLREILPTLFGLLLGFLASTCADKRTIAARTLGDLVRKLGEKILPEIIPILEEGLRSPKSDERQGVCIGLSEIMKSTSRDAVLYFSESLVPTARKALCDPLEEVREAAAKTFEQLHSTIGHQALEDILPFLLKQLDDEEVSEFALDGLKQVMAIKSRVVLPYLVPKLTTPPVNTRVLAFLSSVAGDALTRHLGVILPAVMLALKEKLGTPDEQLEMANCQAVILSVEDDTGHRIIIEDLLEATRSPEVGMRQAAAIILNIYCSRSKADYTSHLRSLVSGLIRLFNDSSPVVLEESWDALNAITKKLDAGNQLALIEELHKEIRFIGNESRGEHVPGFCLPKKGVTSILPVLREGVLTGSPEQKEEAAKALGLVIRLTSADALnullPSVVNITGPLIRILGDRFSWNVKAALLETLSLLLAKVGIALKPFLPQLQTTFTKALQDSNRGVRLKAADALGKLISIHIKVDPLFTELLNGIRVMEDPGnullRDTMLQALRFVIQGAGAKVDVVIRKNIVSLLLSMLGHDEDNTRISnullAGCLGELCAFLTEEELSTVLQQYLLADVSGIDWMVRHGRSLALSVAVSVAPGRLCVGKYGSDVQDMILSNAMADRIPIAVSGVRGMGFLMKHHIETGGGQLPAKLSSLFIKCLQNPSSDIRLVAEKMIWWANKDPLPALDPQAIKPILKALLDNTKDKNTVVRAYSDQAIVNLLKMRQGEEVFQSLCKILDVASLEVLNECNRRSLKKLAGQADSTEQVDDTILT

Target ORF information:

RefSeq Version	XM_019977433.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus GCN1, eIF2 alpha kinase activator homolog (GCN1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019977433.1

ORF Insert Sequence:

ATGGCGGCGG ACACGCAGGT TTCTGAGACA CTAAAGCGTT TTGCAGGGAA GGTGACGACA 
GCCAGTGTGA AGGAACGGAG AGAAATCCTC AGTGAGCTGG GGAAATGTGT TGCCGGAAAA 
GATCTGCCGG AGGGAGCTGT GAAAGGCCTC TGCAAACTCT TCTGCTTGAC CCTGCATCGA 
TACAGGGATG CAGCCTCACG CCGGGCCCTG CAGGCAGCCA TCCAGCAGCT GGCTGAGGCC 
CAGCCTGAAG CCACTGCCAA GAACCTTCTG CACTCTCTGC AGTCCTCGGG CATCGGCTCC 
AAAGCGGGTG TCCCCAGTAA GAGCAGCGGC TCGGCTGCCT TGCTGGCCCT GTCCTGGACT 
GGCCTACTGG TGCGCATCGT CTTTCCCTCG AGAGCCAAGC GGCAAGGAGA CATCTGGAAC 
AAACTGGTGG AGGTGCAGTG CCTGCTCCTG CTGGAGGTGC TGGGTGGCTC CCACAAGCAC 
GCCGTGGACA GTGCYGTGAA GAAGCTCACC AAGCTGTGGA GAGAGAACCC CGGGCTGGTG 
GAGCAGTACT TGTCAGCCAT TCTCAGCCTG GAGCCCAACC AGAGCTACGC GGGCATGCTG 
GGGCTGCTGG TGCAGTTCTG CACGAGCCAC AAGGAGATGG ACGTGGCCAA CCAGCACAAG 
AGTGCYCTGC TGGACTTTTA CGTGAAGAAC ATCCTGATGA GCAAAGTGAA GCCTCAGAAG 
TATCTGTTGG ATAACTGTGC CCCTTTGCTC CGGTACGTGT CCCACTCGGA GTTTAAGGAT 
CTGGTCCTGC CCACCATACA GAAGTCCTTA CTGAGGAGTC CAGAGAATGT CATTGAAACT 
ATCTCCAGTC TGCTGGCATC CGTGACTCTG GATCTCAGCC AGTATGCCCT GGACATTGGG 
AAAGGACTGG CTGGTCAGCT GAAGTCCAAC AGCCCTCGCT TGATGGATGA AGCCGTGCTG 
GCRCTGCGGA ACCTGGCCCG CCAGTGCAGT GACTCCTCGG CGATGGAAGC CCTGGCCAGG 
CACCTGTTCG CTATCCTCGG AGGCTCGGAG GGGAAGCTAA CTATTGTAGC CCAGAAGATG 
AGCGTTCTCT CAGGGATCGG RAGTGTCAGT CATCACGTGG TGTCGGGGCC TTCTAGTCAA 
GTCCTGAGCG GGACCGTGGC TGAGCTCTTC ATCCCGTTCC TCCAGCAAGA AGTTCATGAA 
GGCACCTTGG TGCACGCTGT CTCTGTCTTG GCTCTCTGGT GTCACCGCTT CACCACGGAA 
GTACCCAAGA AGCTCACCGA ATGGTTCAAG AAAGCCTTCA GCCTTAAAAC CTCCACCTCT 
GCCGTGAGGC ACGCCTACCT GCAGTGCATG TTGGTCTGCT TTCGAGGCGA CACACTGCTG 
CAGGCCCTGG ACTTGCTGCC CTTGCTCATC CAGACAGTGG AGAAGGCGGC CTCCCAAGGC 
ACTCAGGTTC CCGTTGTCAC CGAAGGGGTC GCCGCTGCCT TGCTGCTCAC GAAGCTCTCA 
ATGGCCGACT CGCAGGCTGA GGCCAAACTG AGTGGTTTCT GGCAGCTCAT TTTGGATGAG 
AAGAAGCAGG TTTTCACTTC CGAGAAATTC CTCCTCATGG CTTCAGAGGA TGCCCTGTGT 
ACCGTGCTGC ACCTGACAGA GAGACTGTTT CTTGACCATC CGCACCGACT CACAGGCAAC 
AAAGTTCAGC AGTACCACCG GGCCCTGGCG GCCGTGCTCC TGAGCCGCCC CTGGCACGTG 
CGCAGGCAGG CCCAGCAGAC GGCCCGGAAG CTGCTGTCCT CTCTCGGGGG CTTCAAGCTG 
GCTTACGGAC TGCTGGAGGA GCTGAAGACC GTCCTCAGTT CTCACAAGGT GCTGCCCTTC 
GAGGCTCTGG TGACCGACAC TGGGGAGGTG ACTGAGGCGG GCAAGGCCTA CGTGCCCCCG 
CGGGTCCTGC AGGAGGCTCT CTGTGTCATC TCCGGGGTGC CAGGGCTGGA GGGTGGCATC 
ACCGGCATGG AACAGCTGGC ACAGGAGATG CTGATCATCT CTCACCACCC ATCCTTAGTG 
GCTGTGCAGT CTGGCCTCTG GCCAGCACTT CTCGCCAGGA TGAAGATCGA TCCGGAAGCC 
TTCATTAGTA GGCACTTGGA CCAGATCATC CCCAGGATCA CCACCCAGAA CCCCTTGAAC 
CAGTCTTCCA TGAACGCCAT GGGCTCCCTC TCGATCCTGT CCCCAGACCG GGTCCTCCCG 
CAGCTCATCA GCACCATCAC CACCTCCGTG CAGAACCCAG CACTGTGCCA GGTGACGCGG 
GAGGAGTTTG CTATCATGCA GACCCCTGCA GGGGAGTTGT ACGACAAGTC CATCATTCAG 
AGTGCCCAGC AGGACAGCAT AAAAAAGGCC AACATGAAGC GAGAGAACAA AGCTTACTCC 
TTCAAGGAGC AGATCATCGA GTTGGAGCTG AAGGAGGAGA TAAAGAARAA GAAAGGCATA 
AAAGAGGAGG TGCAGCTGAC CAGCAAACAG AAGGAGATGC TGCAGGCCCA GCTGGACAAG 
GAGGCGCAGA TCCGGAGGCG GCTGCAGGAG CTGGACAGTG AGCTGGAGGC GGCTCTGGGG 
CTGCTGGACA CCATCCTGGC CAAGAACCCG TCCGGCCTGA CCCAGTACAT CCCGGTCCTG 
GTCGACTCTT TCCTGCCCTT GCTGAAGTCT CCCCTGGCTG CTCCCAGGAT CAAGACCCCT 
TTCCTGTCCT TGGCTGCCTG TGTCATGCCC CCGAGGCTCA AGGCTTTGGG CACTTTGGTG 
AGCCATGTGA CTCTGCGCCT GCTGAAGCCG GAATGTGCCC TGGACGAGTC ATGGTGCCAG 
GAAGAGCTGT CGGTGGCCGT GAAGAGAGCA GTGACCCTGC TGCACAGTCA CACCATCACC 
AGCAGGGCAG GCAAGGGCGA GCCAGAYGCT GCGCCCCTGT CCGCGCCAGC CTTCTCCTTG 
GTTTTCCCGT TTCTGAAGAT GGTGCTGACT GAGATGCCCC ACCACAGTGA GGAGGAGGAG 
GAAAGAATGG CCCAGATTCT TCAGATCCTC ACAGTCCACG CCCAGCTGCG GGCCTCCCCA 
AGCAACCCAC CTGGGCGTGT GGACGAGAAC GGCCCGGAGT TGCTGCCTCG CGTGGCCATG 
CTGCGCCTTC TGACCTGGGT GATTGGGACG GGCTCTCCCC GACTTCAGGT TCTGGCTTCA 
GAGGCCCTGA CCACCCTGTG TGCCAGCAGC AGTGGCGAGG ATGGCTGTGC CTTTGCAGAG 
CAGGAGGAGG TGGACGTGCT GCTCTGCGCC CTGCTGTCCC CCTGTGCCAA CGTGCGGGAC 
ACTGTACTCC GGGGCCTGTT GGAACTCCAC ATGGTGTTGC CGGCGCCTGA CACCGATGAG 
AAGAATGGCC TGAACCTTCT CCGCAGGCTC TGGGTGGTTA AGTTCGACAA GGAGGAAGAG 
ATCCGAAAGT TGGCCGAGAG GCTCTGGTCG ACCATGGGCC TAGATCTGCA GCCAGACCTC 
TGCTCCTTGC TCATCGAYGA CGTCATCTAT CACGAGGCAG CTGTGAGGCA GGCCGGGGCG 
GAAGCCCTCT CCCAGGCGGT GGCGCGTTAC CAGCGGCAGG CGGCTGAGGT CATGGGCAGG 
CTCATGGAGA TCTACCAGGA GAAGCTCTAT CGGCCGCCCC CGGTGCTGGA TGCTCTGGGA 
CGAGTTATCT CCGAATCTCC TCCAGACCAG TGGGAAGCCA GGTGTGGCTT GGCTTTGGCC 
CTGAACAAGC TCTCCCAGTG TTTGGACAGC TCTCAGGTGA AGCCACTCTT TCAATTTTTT 
GTCCCCGACG CCCTCAACGA CCGAAACCCA GATGTCCGGA AGTGCATGTT GGATGCAGCT 
CTTGCGACAC TCAACACACA TGGAAAGGAG AACGTCAACT CGCTGCTGCC GGTGTTCGAG 
GAGTTCCTGA AGAACGCGCC GAACGATGCC AGCTACGATG CCGTGCGGCA GAGCGTGGTG 
GTCCTCATGG GCTCTCTAGC CAAGCACCTG GACAAGAGTG ACCCCAAAGT GAAGCCGATT 
GTCGCCAAGC TCATTGCTGC CCTCGCCACC CCCTCCCAGC AGGTCCAGGA ATCCGTGGCC 
AGCTGCTTGC CACCCCTCGT CCCTGCCATA AAGGAAGACG CGGGGGGTAT GATCCAGAGG 
CTGATGCAGC AGCTGCTGGA GTCAGACAAG TACGCCGAGC GCAAAGGGGC TGCGTACGGG 
CTGGCGGGCC TGGTGAAGGG CCTGGGCATC CTGTCGCTGA AGCAGCAGGA GATGATGGCC 
GCGCTGACCG ATGCCATTCA GGACAAGAAG AACTTCCGCC GCCGGGAGGG AGCCCTCTTT 
GCCTTCGAGA TGCTCTGCAC CATGCTGGGG AAGCTCTTTG AGCCGTATGT GGTCCACGTG 
CTGCCCCATC TGCTGCTCTG CTTTGGGGAC GGGAACCAGT ATGTGCGGGA GGCTGCAGAT 
GACTGTGCCA AGGCTGTGAT GAGCAACCTG AGTGCTCATG GGGTGAAGCT GGTGCTCCCC 
TCCTTGTTGG CTGCCCTGGA GGAGGAATCC TGGAGAACCA AAGCCGGGTC GGTGGAGTTG 
CTCGGGGCCA TGGCCTACTG TGCTCCCAAG CAGCTGTCGT CCTGCCTACC CAACATCGTG 
CCCAAGCTCA CAGAGGTGCT CACCGACTCC CACGTCAAAG TGCARAAGGC TGGGCAGCAG 
GCGCTCAGGC AGATCGGCTC TGTCATCAGG AACCCAGAGA TCCTGGCCAT CGCCCCGGTA 
CTGCTGGACG CCCTGACAGA CCCCTCCCGG AAGACCCAGA AGTGCTTGCA GACCTTGCTG 
GACACCAAGT TTGTCCACTT CATCGATGCA CCGTCCCTGG CCCTCATCAT GCCCATCGTC 
CAGAGAGCCT TCCAGGACCG TTCCACGGAC ACACGGAAGA TGGCCGCCCA GATCATTGGC 
AACATGTACT CCCTGACAGA CCAGAAGGAC TTGGCTCCTT ACCTGCCAAG CGTGACGCCT 
GGTCTGAAAG CCTCTCTCCT GGATCCTGTG CCTGAGGTAC GGACAGTGTC TGCAAAGGCT 
CTTGGGGCCA TGGTCAAGGG CATGGGGGAG TCATGCTTTG AGGACTTGCT GCCGTGGCTG 
ATGGAGACAC TGACCTACGA GCAGAGCTCT GTGGATCGCT CAGGCGCCGC GCAGGGGTTG 
GCTGAGGTCA TGGCCGGCTT GGGGGTGGAG AAGTTGGAGA AGCTGATGCC AGAAATTGTG 
GCTACAGCCA GCAAAGTGGA CATTGCCCCC CACGTCCGAG ACGGCTACAT CATGATGTTC 
AACTACCTGC CCATCACCTT CGGGGACAAA TTCACTCCTT ATGTGGGGCC CATCATCCCC 
TGTATCCTCA AAGCTCTTGC TGATGAGAAC GAGTTTGTGC GTGATACTGC CCTGCGTGCA 
GGCCAGCGGG TCATCTCCAT GTATGCTGAG ACGGCCATTG CCCTGCTGCT GCCCCAGCTG 
GAGCAAGGCC TCTTTGATGA CCTCTGGAGA ATAAGGTTCA GCTCTGTTCA GCTCCTTGGC 
GATCTCCTGT TCCACATCTC AGGAGTCACT GGGAAGATGA CCACAGAAAC TGCCTCAGAG 
GATGACAACT TTGGAACCGC GCAGTCCAAC AAGGCGATTA TCACCGCCCT GGGGGTAGAT 
CGGCGGAACC GGGTGTTGGC GGGGCTGTAC ATGGGCCGCT CGGACACCCA GCTGGTGGTG 
CGGCAGGCCT CCCTGCACGT CTGGAAGATT GTGGTCTCTA ACACCCCCCG CACCTTGCGT 
GAGATCCTTC CCACCCTTTT TGGGCTCCTG CTGGGTTTCC TGGCCAGCAC ATGCGCGGAT 
AAGAGAACAA TCGCAGCCAG GACGCTGGGA GATCTCGTCC GGAAGTTAGG GGAGAAAATC 
CTGCCGGAGA TCATCCCCAT CCTGGAGGAA GGCCTGAGAT CTCCCAAGAG TGACGAGAGG 
CAGGGCGTGT GCATCGGGCT GAGCGAGATC ATGAAGTCCA CCAGCCGGGA TGCGGTGCTG 
TATTTCTCCG AGTCCCTCGT GCCCACGGCG AGGAAGGCTT TGTGTGACCC GCTGGAGGAG 
GTCAGAGAGG CGGCGGCCAA GACGTTTGAG CAGCTGCACT CCACCATCGG CCACCAGGCC 
CTGGAGGACA TCCTCCCGTT TTTACTGAAG CAGCTGGATG ACGAGGAGGT GTCCGAGTTT 
GCCTTGGATG GTCTGAAGCA GGTGATGGCC ATCAAGAGTC GTGTGGTGCT GCCCTACCTC 
GTGCCCAAGC TGACGACCCC GCCCGTCAAC ACCCGGGTGC TGGCTTTCCT TTCATCTGTG 
GCGGGTGATG CCCTGACACG TCATCTTGGC GTGATCCTCC CGGCGGTCAT GTTGGCCCTG 
AAGGAGAAGC TGGGGACTCC AGACGAGCAG CTGGAGATGG CCAACTGTCA GGCCGTGATC 
CTGTCCGTGG AGGACGACAC CGGGCACCGG ATCATCATCG AGGACCTGTT GGAAGCCACC 
CGCAGCCCTG AGGTGGGCAT GAGGCAGGCC GCGGCCATCA TCCTCAACAT CTACTGCTCA 
CGCTCCAAGG CGGACTACAC TAGCCACCTG CGGAGCCTGG TCTCAGGCCT GATCCGCCTC 
TTCAACGACT CCAGCCCCGT GGTTCTGGAG GAGAGCTGGG ACGCCCTGAA TGCCATCACC 
AAGAAACTGG ATGCAGGCAA CCAGCTGGCC CTCATTGAGG AACTGCACAA GGAAATCCGG 
TTCATAGGAA ATGAGAGCAG AGGCGAGCAC GTGCCAGGCT TCTGCCTCCC GAAGAAGGGG 
GTAACGTCCA TCCTTCCAGT GCTGCGGGAG GGGGTCCTGA CTGGCAGCCC GGAACAGAAG 
GAGGAAGCTG CCAAAGCGTT AGGCTTGGTG ATCCGCCTGA CCTCGGCGGA CGCCCTGMGG 
CCATCCGTGG TCAACATCAC TGGCCCTCTG ATTCGCATCC TGGGGGACCG GTTCAGCTGG 
AACGTGAAGG CGGCTCTGCT CGAGACACTC AGCCTCTTGC TGGCCAAGGT CGGGATCGCC 
CTGAAGCCCT TCCTGCCCCA GCTGCAGACC ACCTTCACCA AAGCCCTGCA GGACTCTAAC 
CGCGGCGTGC GCCTGAAGGC CGCCGATGCG CTAGGAAAGC TCATTTCCAT CCACATCAAG 
GTGGATCCTC TTTTCACGGA GCTGCTGAAT GGCATCCGGG TCATGGAGGA CCCAGGCRTC 
AGGGACACCA TGCTGCAGGC CCTGAGGTTT GTGATTCAGG GAGCGGGGGC CAAAGTGGAT 
GTGGTCATCC GGAAAAACAT CGTCTCGCTC CTGCTGAGCA TGCTGGGACA CGACGAGGAC 
AACACGCGCA TCTCCTCRGC CGGGTGCCTG GGGGAGCTAT GTGCCTTTCT GACGGAAGAG 
GAGCTCAGCA CTGTTCTTCA GCAGTACTTG CTGGCGGATG TGTCTGGCAT TGACTGGATG 
GTTCGGCATG GGCGGAGCCT GGCTCTCTCC GTGGCTGTGA GCGTGGCTCC TGGCAGACTC 
TGCGTAGGCA AGTATGGCAG CGACGTCCAG GACATGATCC TCAGCAATGC CATGGCAGAC 
AGGATCCCCA TCGCCGTGAG TGGGGTCCGG GGCATGGGCT TCCTGATGAA GCACCACATC 
GAGACCGGAG GAGGACAGTT GCCGGCCAAA CTCTCCAGCC TGTTCATTAA GTGTCTGCAG 
AACCCATCCA GTGACATCAG GTTGGTGGCT GAGAAGATGA TCTGGTGGGC AAACAAGGAC 
CCGCTGCCTG CCCTGGACCC CCAGGCCATC AAGCCCATCC TGAAGGCCCT CCTGGACAAC 
ACCAAGGATA AAAACACCGT CGTCAGGGCC TACAGCGACC AGGCGATTGT CAACCTCCTC 
AAGATGAGGC AGGGAGAGGA GGTGTTTCAG TCCCTCTGCA AGATCCTGGA TGTGGCCAGC 
TTGGAAGTGC TGAATGAATG CAACCGGAGG TCTCTGAAGA AGCTGGCCGG CCAGGCTGAC 
TCCACAGAGC AGGTGGACGA CACCATCCTG ACCTGA