AAK1 gene cDNA ORF clone, Bos indicus(Bos taurus indicus)

mRNA	Protein	Name
XM_019970439.1	XP_019825998.1	AP2-associated protein kinase 1 isoform X3
XM_019970445.1	XP_019826004.1	AP2-associated protein kinase 1 isoform X9
XM_019970447.1	XP_019826006.1	AP2-associated protein kinase 1 isoform X11
XM_019970437.1	XP_019825996.1	AP2-associated protein kinase 1 isoform X1
XM_019970436.1	XP_019825995.1	AP2-associated protein kinase 1 isoform X1
XM_019970440.1	XP_019825999.1	AP2-associated protein kinase 1 isoform X4
XM_019970442.1	XP_019826001.1	AP2-associated protein kinase 1 isoform X6
XM_019970446.1	XP_019826005.1	AP2-associated protein kinase 1 isoform X10
XM_019970438.1	XP_019825997.1	AP2-associated protein kinase 1 isoform X2
XM_019970441.1	XP_019826000.1	AP2-associated protein kinase 1 isoform X5
XM_019970449.1	XP_019826008.1	AP2-associated protein kinase 1 isoform X13
XM_019970443.1	XP_019826002.1	AP2-associated protein kinase 1 isoform X7
XM_019970444.1	XP_019826003.1	AP2-associated protein kinase 1 isoform X8
XM_019970448.1	XP_019826007.1	AP2-associated protein kinase 1 isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBo29217	XM_019970442.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo29218	XM_019970443.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo29212	XM_019970437.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBo29212	XM_019970436.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBo29213	XM_019970438.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBo29214	XM_019970439.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBo29215	XM_019970440.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBo29216	XM_019970441.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBo29219	XM_019970444.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBo29220	XM_019970445.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBo29221	XM_019970446.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBo29222	XM_019970447.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBo29223	XM_019970448.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBo29224	XM_019970449.1 Latest version!	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

AAK1 ( XM_019970442.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826001.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBo29217
Clone ID Related Accession (Same CDS sequence)	XM_019970442.1
Accession Version	XM_019970442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC 
ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT 
GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC 
AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT 
GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA 
GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA 
CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC 
TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC 
TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT 
CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA 
CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG 
ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG 
AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT 
GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG 
CTTTCTAACC CCACTACTGA CCTYCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG 
GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG 
GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA 
GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGARTCG 
ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT 
CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC 
CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT 
CGCCCTCTRC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG 
CTAGGAGGAG CTACTCCCTT CACTCAGCCS GAGGTCACTC CTGCGCAGGC CAAGGCCTCR 
CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT 
GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT 
TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACK 
GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC 
CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC 
CCGRGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT 
GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC 
TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT 
CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT 
TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC 
ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC 
ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA 
CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826001.1
CDS	392..4027
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDnullLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970442.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970442.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGCAGATTC AAGCCCCAGT AAGACAACAG CCAAAGGTTC AGACAACCCC ACCTCCTACC 
ATCCAGGGGC AGAAACTCGG ATCTCTCACT CCTCCATCAT CCCCCAAAGC CCAGCGTGCT 
GGGCACAGGC GAATTCTCAG CGACGTGACC CACAGCGCAG TCTTTGGGGT CCCTGCCAGC 
AAATCAACCC AGCTGCTCCA AGCAGCTGCA GCTGAGGCCA GTCTCAATAA GTCCAAGTCT 
GCAACCACCA CTCCTTCAGG CTCCCCTCGG GCCTCCCAGC AAAATGTTTA TAATCCTTCA 
GAAGGTTCTA CCTGGAATCC CTTTGATGAC GACAATTTCT CCAAACTGAC AGCTGAAGAA 
CTGCTAAACA AGGACTTTGC CAAGCTGGGG GAAGGCAAGT ATCCTGAGAA GCTTGGAGGC 
TCTGCTGAGA GTTTGATCCC AGGCTTTCAA CCAACCCAAG GTGATGCTTT TGCTGCTTCC 
TCATTTTCTG CTGGAACTGC TGAAAAAAGG AAGGGTGGGC AGACTATGGA TTCTAGCCTT 
CCTCTTCTAA GTGTATCTGA TCCTTTCATT CCTCTTCAAG TACCTGATGC ACCAGAAAAA 
CTAATTGAGG GACTCAAATC TCCTGACACT TCTCTTCTGC TCCCTGACCT CTTGCCTATG 
ACAGATCCTT TTGGTAGCAC TTCTGATGCT GTAATTGAAA AAGCTGATGT TGCTGTTGAG 
AGTCTGATAC CAGGACTGGA GCCCCCGGTG CCTCAGCGCC TTCCATCTCA GACGGAATCT 
GTGACCTCGA ACCGCACAGA TTCTCTCACC GGGGAAGATT CCCTGATTGA TTGCTCTCTG 
CTTTCTAACC CCACTACTGA CCTYCTGGAA GAGTTTGCCC CCATAGCCAT CTCTGCTCCG 
GCCCATAAAG CTGCGGAAGA TAGTAATCTC ATCTCAGGTT TTGATGTCCC TGAGGGCTCG 
GACAAGGTGG CAGAAGATGA GTTTGACCCT ATTCCTGTAT TGATAACCAA AAACCCACAA 
GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT GTAATAACTG TAGAGCACTT TAAAGARTCG 
ACGGGTGTTA AAGGCCTTCC CCTATACCCG GACCCCTCCC GGGTACCTGG CACGAAAACT 
CAGAACAGTT TGGAATCTGA CTACTTGGCC CGAGATGGCC CTTCCAGCAA CAGCTCGTTC 
CACAGCAGCG AAGAGGAGGG GACCGACCTC GAGGGGGACA TGCTGGACTG CAGTGGCTCT 
CGCCCTCTRC TTTTGGAGTC AGAAGAAGAG GATGAGAGCT GCAAGCCTCC GCAGGGGAAG 
CTAGGAGGAG CTACTCCCTT CACTCAGCCS GAGGTCACTC CTGCGCAGGC CAAGGCCTCR 
CAAGGTGGGA GGAAGAACCA ATTCGGAGCC CTCATTCAGC CAATTGCAGA TGGACTCGGT 
GAGCCAGATG TGTTCGCCAC TGCCCCCTTC CGGAGCTCAA GGATTCCCGC TGATGACATT 
TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC AACGTGGCCC CTTCCCAGCC AGAGGAGACK 
GATGTGTTCT TGAGAGCTCC CTTTACCAAG AAGAAAGGCA TGGAGGAGTC AACAGTTGCC 
CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC CTCAGTCAGA CCGATGACAT CCCTCTGCTC 
CCGRGCCTCG ATCGAGCCGT GTACCCGTCT GCCCGAGCTC AGTATTCCAT GGCTGGCTTT 
GCCCAGCCGT CTAACCTCCC CTCCCATCCT GTACAAGCAG CAGACCATCT TGACAGCATC 
TCTTCCAGGG GCTCCTCCCT GGAATCTGGG GCCCATCCTA ATGACAGAAA CAAAGGACCT 
CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC GTGGCAGGCA AACCATTCCG CCCCCAGTCT 
TTATCCAAAT ATTCCCGTCA CTATAGCCCG GAAGATGAGC CGAATCCAGA AGCCCAGCCC 
ATCGCTGCCT ACAAAATTGT TTCGCAAACC AATAAGCAGT CGATAGCTGG CTCCGTTTCC 
ATCACATCCC TTGCCTCCAG GACTACGGAG CTGCCAGCTG CTGATCCTTT TGCTTTAGCA 
CCCTTTCCTT CGAAATCAGG CAAACAGAAA CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970443.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826002.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBo29218
Clone ID Related Accession (Same CDS sequence)	XM_019970443.1
Accession Version	XM_019970443.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC 
CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG 
CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA 
TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA 
ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA 
GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG 
CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT 
GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA 
TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT 
CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA 
ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA 
GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT 
CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG 
ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT 
TCTAACCCCA CTACTGACCT YCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC 
CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC 
AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGARTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTRCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCSGAG GTCACTCCTG CGCAGGCCAA GGCCTCRCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACKGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
RGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826002.1
CDS	392..4024
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDnullLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970443.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970443.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGATTCAAG CCCCAGTAAG ACAACAGCCA AAGGTTCAGA CAACCCCACC TCCTACCATC 
CAGGGGCAGA AACTCGGATC TCTCACTCCT CCATCATCCC CCAAAGCCCA GCGTGCTGGG 
CACAGGCGAA TTCTCAGCGA CGTGACCCAC AGCGCAGTCT TTGGGGTCCC TGCCAGCAAA 
TCAACCCAGC TGCTCCAAGC AGCTGCAGCT GAGGCCAGTC TCAATAAGTC CAAGTCTGCA 
ACCACCACTC CTTCAGGCTC CCCTCGGGCC TCCCAGCAAA ATGTTTATAA TCCTTCAGAA 
GGTTCTACCT GGAATCCCTT TGATGACGAC AATTTCTCCA AACTGACAGC TGAAGAACTG 
CTAAACAAGG ACTTTGCCAA GCTGGGGGAA GGCAAGTATC CTGAGAAGCT TGGAGGCTCT 
GCTGAGAGTT TGATCCCAGG CTTTCAACCA ACCCAAGGTG ATGCTTTTGC TGCTTCCTCA 
TTTTCTGCTG GAACTGCTGA AAAAAGGAAG GGTGGGCAGA CTATGGATTC TAGCCTTCCT 
CTTCTAAGTG TATCTGATCC TTTCATTCCT CTTCAAGTAC CTGATGCACC AGAAAAACTA 
ATTGAGGGAC TCAAATCTCC TGACACTTCT CTTCTGCTCC CTGACCTCTT GCCTATGACA 
GATCCTTTTG GTAGCACTTC TGATGCTGTA ATTGAAAAAG CTGATGTTGC TGTTGAGAGT 
CTGATACCAG GACTGGAGCC CCCGGTGCCT CAGCGCCTTC CATCTCAGAC GGAATCTGTG 
ACCTCGAACC GCACAGATTC TCTCACCGGG GAAGATTCCC TGATTGATTG CTCTCTGCTT 
TCTAACCCCA CTACTGACCT YCTGGAAGAG TTTGCCCCCA TAGCCATCTC TGCTCCGGCC 
CATAAAGCTG CGGAAGATAG TAATCTCATC TCAGGTTTTG ATGTCCCTGA GGGCTCGGAC 
AAGGTGGCAG AAGATGAGTT TGACCCTATT CCTGTATTGA TAACCAAAAA CCCACAAGGT 
CTCCAGAGAG AGCCCATTTC CTCTCCTGTA ATAACTGTAG AGCACTTTAA AGARTCGACG 
GGTGTTAAAG GCCTTCCCCT ATACCCGGAC CCCTCCCGGG TACCTGGCAC GAAAACTCAG 
AACAGTTTGG AATCTGACTA CTTGGCCCGA GATGGCCCTT CCAGCAACAG CTCGTTCCAC 
AGCAGCGAAG AGGAGGGGAC CGACCTCGAG GGGGACATGC TGGACTGCAG TGGCTCTCGC 
CCTCTRCTTT TGGAGTCAGA AGAAGAGGAT GAGAGCTGCA AGCCTCCGCA GGGGAAGCTA 
GGAGGAGCTA CTCCCTTCAC TCAGCCSGAG GTCACTCCTG CGCAGGCCAA GGCCTCRCAA 
GGTGGGAGGA AGAACCAATT CGGAGCCCTC ATTCAGCCAA TTGCAGATGG ACTCGGTGAG 
CCAGATGTGT TCGCCACTGC CCCCTTCCGG AGCTCAAGGA TTCCCGCTGA TGACATTTTT 
GCCAAAGCCC CCTTTGTCTC CAAGGGCAAC GTGGCCCCTT CCCAGCCAGA GGAGACKGAT 
GTGTTCTTGA GAGCTCCCTT TACCAAGAAG AAAGGCATGG AGGAGTCAAC AGTTGCCCAG 
AGCCCCGCCC AGGAGCAGTC CGGTCTCCTC AGTCAGACCG ATGACATCCC TCTGCTCCCG 
RGCCTCGATC GAGCCGTGTA CCCGTCTGCC CGAGCTCAGT ATTCCATGGC TGGCTTTGCC 
CAGCCGTCTA ACCTCCCCTC CCATCCTGTA CAAGCAGCAG ACCATCTTGA CAGCATCTCT 
TCCAGGGGCT CCTCCCTGGA ATCTGGGGCC CATCCTAATG ACAGAAACAA AGGACCTCAG 
CCCCAGAAAG AAGCTGTCTC AGGCCCCGTG GCAGGCAAAC CATTCCGCCC CCAGTCTTTA 
TCCAAATATT CCCGTCACTA TAGCCCGGAA GATGAGCCGA ATCCAGAAGC CCAGCCCATC 
GCTGCCTACA AAATTGTTTC GCAAACCAAT AAGCAGTCGA TAGCTGGCTC CGTTTCCATC 
ACATCCCTTG CCTCCAGGAC TACGGAGCTG CCAGCTGCTG ATCCTTTTGC TTTAGCACCC 
TTTCCTTCGA AATCAGGCAA ACAGAAACCT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970437.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019825996.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

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CloneID	OBo29212
Clone ID Related Accession (Same CDS sequence)	XM_019970437.1 , XM_019970436.1
Accession Version	XM_019970437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTYCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGARTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTR CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC SGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC RCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC KGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGRGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019825996.1
CDS	505..4401
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDnullLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970437.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970437.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTYCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGARTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTR CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC SGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC RCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC KGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGRGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970436.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019825995.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo29212
Clone ID Related Accession (Same CDS sequence)	XM_019970437.1 , XM_019970436.1
Accession Version	XM_019970436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTYCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGARTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTR CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC SGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC RCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC KGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGRGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019825995.1
CDS	392..4288
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDnullLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970436.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970436.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTYCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTCTCCAG AGAGAGCCCA TTTCCTCTCC TGTAATAACT 
GTAGAGCACT TTAAAGARTC GACGGGTGTT AAAGGCCTTC CCCTATACCC GGACCCCTCC 
CGGGTACCTG GCACGAAAAC TCAGAACAGT TTGGAATCTG ACTACTTGGC CCGAGATGGC 
CCTTCCAGCA ACAGCTCGTT CCACAGCAGC GAAGAGGAGG GGACCGACCT CGAGGGGGAC 
ATGCTGGACT GCAGTGGCTC TCGCCCTCTR CTTTTGGAGT CAGAAGAAGA GGATGAGAGC 
TGCAAGCCTC CGCAGGGGAA GCTAGGAGGA GCTACTCCCT TCACTCAGCC SGAGGTCACT 
CCTGCGCAGG CCAAGGCCTC RCAAGGTGGG AGGAAGAACC AATTCGGAGC CCTCATTCAG 
CCAATTGCAG ATGGACTCGG TGAGCCAGAT GTGTTCGCCA CTGCCCCCTT CCGGAGCTCA 
AGGATTCCCG CTGATGACAT TTTTGCCAAA GCCCCCTTTG TCTCCAAGGG CAACGTGGCC 
CCTTCCCAGC CAGAGGAGAC KGATGTGTTC TTGAGAGCTC CCTTTACCAA GAAGAAAGGC 
ATGGAGGAGT CAACAGTTGC CCAGAGCCCC GCCCAGGAGC AGTCCGGTCT CCTCAGTCAG 
ACCGATGACA TCCCTCTGCT CCCGRGCCTC GATCGAGCCG TGTACCCGTC TGCCCGAGCT 
CAGTATTCCA TGGCTGGCTT TGCCCAGCCG TCTAACCTCC CCTCCCATCC TGTACAAGCA 
GCAGACCATC TTGACAGCAT CTCTTCCAGG GGCTCCTCCC TGGAATCTGG GGCCCATCCT 
AATGACAGAA ACAAAGGACC TCAGCCCCAG AAAGAAGCTG TCTCAGGCCC CGTGGCAGGC 
AAACCATTCC GCCCCCAGTC TTTATCCAAA TATTCCCGTC ACTATAGCCC GGAAGATGAG 
CCGAATCCAG AAGCCCAGCC CATCGCTGCC TACAAAATTG TTTCGCAAAC CAATAAGCAG 
TCGATAGCTG GCTCCGTTTC CATCACATCC CTTGCCTCCA GGACTACGGA GCTGCCAGCT 
GCTGATCCTT TTGCTTTAGC ACCCTTTCCT TCGAAATCAG GCAAACAGAA ACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970438.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019825997.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBo29213
Clone ID Related Accession (Same CDS sequence)	XM_019970438.1
Accession Version	XM_019970438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TYCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGARTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTRCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCSGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCRCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACKGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GRGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019825997.1
CDS	392..4285
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDnullLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970438.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970438.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TYCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TCTCCAGAGA GAGCCCATTT CCTCTCCTGT AATAACTGTA 
GAGCACTTTA AAGARTCGAC GGGTGTTAAA GGCCTTCCCC TATACCCGGA CCCCTCCCGG 
GTACCTGGCA CGAAAACTCA GAACAGTTTG GAATCTGACT ACTTGGCCCG AGATGGCCCT 
TCCAGCAACA GCTCGTTCCA CAGCAGCGAA GAGGAGGGGA CCGACCTCGA GGGGGACATG 
CTGGACTGCA GTGGCTCTCG CCCTCTRCTT TTGGAGTCAG AAGAAGAGGA TGAGAGCTGC 
AAGCCTCCGC AGGGGAAGCT AGGAGGAGCT ACTCCCTTCA CTCAGCCSGA GGTCACTCCT 
GCGCAGGCCA AGGCCTCRCA AGGTGGGAGG AAGAACCAAT TCGGAGCCCT CATTCAGCCA 
ATTGCAGATG GACTCGGTGA GCCAGATGTG TTCGCCACTG CCCCCTTCCG GAGCTCAAGG 
ATTCCCGCTG ATGACATTTT TGCCAAAGCC CCCTTTGTCT CCAAGGGCAA CGTGGCCCCT 
TCCCAGCCAG AGGAGACKGA TGTGTTCTTG AGAGCTCCCT TTACCAAGAA GAAAGGCATG 
GAGGAGTCAA CAGTTGCCCA GAGCCCCGCC CAGGAGCAGT CCGGTCTCCT CAGTCAGACC 
GATGACATCC CTCTGCTCCC GRGCCTCGAT CGAGCCGTGT ACCCGTCTGC CCGAGCTCAG 
TATTCCATGG CTGGCTTTGC CCAGCCGTCT AACCTCCCCT CCCATCCTGT ACAAGCAGCA 
GACCATCTTG ACAGCATCTC TTCCAGGGGC TCCTCCCTGG AATCTGGGGC CCATCCTAAT 
GACAGAAACA AAGGACCTCA GCCCCAGAAA GAAGCTGTCT CAGGCCCCGT GGCAGGCAAA 
CCATTCCGCC CCCAGTCTTT ATCCAAATAT TCCCGTCACT ATAGCCCGGA AGATGAGCCG 
AATCCAGAAG CCCAGCCCAT CGCTGCCTAC AAAATTGTTT CGCAAACCAA TAAGCAGTCG 
ATAGCTGGCT CCGTTTCCAT CACATCCCTT GCCTCCAGGA CTACGGAGCT GCCAGCTGCT 
GATCCTTTTG CTTTAGCACC CTTTCCTTCG AAATCAGGCA AACAGAAACC TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970439.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019825998.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

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CloneID	OBo29214
Clone ID Related Accession (Same CDS sequence)	XM_019970439.1
Accession Version	XM_019970439.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA 
AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT 
TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT 
GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT 
GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC 
CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT 
CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTY 
CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT 
AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT 
GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC 
TCTCCTGTAA TAACTGTAGA GCACTTTAAA GARTCGACGG GTGTTAAAGG CCTTCCCCTA 
TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC 
TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC 
GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTRCTTTT GGAGTCAGAA 
GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT 
CAGCCSGAGG TCACTCCTGC GCAGGCCAAG GCCTCRCAAG GTGGGAGGAA GAACCAATTC 
GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC 
CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC 
AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACKGATG TGTTCTTGAG AGCTCCCTTT 
ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC 
GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGR GCCTCGATCG AGCCGTGTAC 
CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC 
CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA 
TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA 
GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT 
AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG 
CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT 
ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA 
CAGAAACCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019825998.1
CDS	393..4184
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDnullLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970439.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970439.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGCTGAA 
AAAAGGAAGG GTGGGCAGAC TATGGATTCT AGCCTTCCTC TTCTAAGTGT ATCTGATCCT 
TTCATTCCTC TTCAAGTACC TGATGCACCA GAAAAACTAA TTGAGGGACT CAAATCTCCT 
GACACTTCTC TTCTGCTCCC TGACCTCTTG CCTATGACAG ATCCTTTTGG TAGCACTTCT 
GATGCTGTAA TTGAAAAAGC TGATGTTGCT GTTGAGAGTC TGATACCAGG ACTGGAGCCC 
CCGGTGCCTC AGCGCCTTCC ATCTCAGACG GAATCTGTGA CCTCGAACCG CACAGATTCT 
CTCACCGGGG AAGATTCCCT GATTGATTGC TCTCTGCTTT CTAACCCCAC TACTGACCTY 
CTGGAAGAGT TTGCCCCCAT AGCCATCTCT GCTCCGGCCC ATAAAGCTGC GGAAGATAGT 
AATCTCATCT CAGGTTTTGA TGTCCCTGAG GGCTCGGACA AGGTGGCAGA AGATGAGTTT 
GACCCTATTC CTGTATTGAT AACCAAAAAC CCACAAGGTC TCCAGAGAGA GCCCATTTCC 
TCTCCTGTAA TAACTGTAGA GCACTTTAAA GARTCGACGG GTGTTAAAGG CCTTCCCCTA 
TACCCGGACC CCTCCCGGGT ACCTGGCACG AAAACTCAGA ACAGTTTGGA ATCTGACTAC 
TTGGCCCGAG ATGGCCCTTC CAGCAACAGC TCGTTCCACA GCAGCGAAGA GGAGGGGACC 
GACCTCGAGG GGGACATGCT GGACTGCAGT GGCTCTCGCC CTCTRCTTTT GGAGTCAGAA 
GAAGAGGATG AGAGCTGCAA GCCTCCGCAG GGGAAGCTAG GAGGAGCTAC TCCCTTCACT 
CAGCCSGAGG TCACTCCTGC GCAGGCCAAG GCCTCRCAAG GTGGGAGGAA GAACCAATTC 
GGAGCCCTCA TTCAGCCAAT TGCAGATGGA CTCGGTGAGC CAGATGTGTT CGCCACTGCC 
CCCTTCCGGA GCTCAAGGAT TCCCGCTGAT GACATTTTTG CCAAAGCCCC CTTTGTCTCC 
AAGGGCAACG TGGCCCCTTC CCAGCCAGAG GAGACKGATG TGTTCTTGAG AGCTCCCTTT 
ACCAAGAAGA AAGGCATGGA GGAGTCAACA GTTGCCCAGA GCCCCGCCCA GGAGCAGTCC 
GGTCTCCTCA GTCAGACCGA TGACATCCCT CTGCTCCCGR GCCTCGATCG AGCCGTGTAC 
CCGTCTGCCC GAGCTCAGTA TTCCATGGCT GGCTTTGCCC AGCCGTCTAA CCTCCCCTCC 
CATCCTGTAC AAGCAGCAGA CCATCTTGAC AGCATCTCTT CCAGGGGCTC CTCCCTGGAA 
TCTGGGGCCC ATCCTAATGA CAGAAACAAA GGACCTCAGC CCCAGAAAGA AGCTGTCTCA 
GGCCCCGTGG CAGGCAAACC ATTCCGCCCC CAGTCTTTAT CCAAATATTC CCGTCACTAT 
AGCCCGGAAG ATGAGCCGAA TCCAGAAGCC CAGCCCATCG CTGCCTACAA AATTGTTTCG 
CAAACCAATA AGCAGTCGAT AGCTGGCTCC GTTTCCATCA CATCCCTTGC CTCCAGGACT 
ACGGAGCTGC CAGCTGCTGA TCCTTTTGCT TTAGCACCCT TTCCTTCGAA ATCAGGCAAA 
CAGAAACCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970440.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019825999.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBo29215
Clone ID Related Accession (Same CDS sequence)	XM_019970440.1
Accession Version	XM_019970440.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC 
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT 
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGARTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTRC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCS 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCR CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACK GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGRGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019825999.1
CDS	392..4177
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970440.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970440.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTGCGGAAGA TAGTAATCTC 
ATCTCAGGTT TTGATGTCCC TGAGGGCTCG GACAAGGTGG CAGAAGATGA GTTTGACCCT 
ATTCCTGTAT TGATAACCAA AAACCCACAA GGTCTCCAGA GAGAGCCCAT TTCCTCTCCT 
GTAATAACTG TAGAGCACTT TAAAGARTCG ACGGGTGTTA AAGGCCTTCC CCTATACCCG 
GACCCCTCCC GGGTACCTGG CACGAAAACT CAGAACAGTT TGGAATCTGA CTACTTGGCC 
CGAGATGGCC CTTCCAGCAA CAGCTCGTTC CACAGCAGCG AAGAGGAGGG GACCGACCTC 
GAGGGGGACA TGCTGGACTG CAGTGGCTCT CGCCCTCTRC TTTTGGAGTC AGAAGAAGAG 
GATGAGAGCT GCAAGCCTCC GCAGGGGAAG CTAGGAGGAG CTACTCCCTT CACTCAGCCS 
GAGGTCACTC CTGCGCAGGC CAAGGCCTCR CAAGGTGGGA GGAAGAACCA ATTCGGAGCC 
CTCATTCAGC CAATTGCAGA TGGACTCGGT GAGCCAGATG TGTTCGCCAC TGCCCCCTTC 
CGGAGCTCAA GGATTCCCGC TGATGACATT TTTGCCAAAG CCCCCTTTGT CTCCAAGGGC 
AACGTGGCCC CTTCCCAGCC AGAGGAGACK GATGTGTTCT TGAGAGCTCC CTTTACCAAG 
AAGAAAGGCA TGGAGGAGTC AACAGTTGCC CAGAGCCCCG CCCAGGAGCA GTCCGGTCTC 
CTCAGTCAGA CCGATGACAT CCCTCTGCTC CCGRGCCTCG ATCGAGCCGT GTACCCGTCT 
GCCCGAGCTC AGTATTCCAT GGCTGGCTTT GCCCAGCCGT CTAACCTCCC CTCCCATCCT 
GTACAAGCAG CAGACCATCT TGACAGCATC TCTTCCAGGG GCTCCTCCCT GGAATCTGGG 
GCCCATCCTA ATGACAGAAA CAAAGGACCT CAGCCCCAGA AAGAAGCTGT CTCAGGCCCC 
GTGGCAGGCA AACCATTCCG CCCCCAGTCT TTATCCAAAT ATTCCCGTCA CTATAGCCCG 
GAAGATGAGC CGAATCCAGA AGCCCAGCCC ATCGCTGCCT ACAAAATTGT TTCGCAAACC 
AATAAGCAGT CGATAGCTGG CTCCGTTTCC ATCACATCCC TTGCCTCCAG GACTACGGAG 
CTGCCAGCTG CTGATCCTTT TGCTTTAGCA CCCTTTCCTT CGAAATCAGG CAAACAGAAA 
CCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970441.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826000.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBo29216
Clone ID Related Accession (Same CDS sequence)	XM_019970441.1
Accession Version	XM_019970441.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGARTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTRCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCSG AGGTCACTCC TGCGCAGGCC AAGGCCTCRC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACKG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGRGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826000.1
CDS	392..4066
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970441.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970441.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGARTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTRCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCSG AGGTCACTCC TGCGCAGGCC AAGGCCTCRC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACKG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGRGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970444.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826003.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBo29219
Clone ID Related Accession (Same CDS sequence)	XM_019970444.1
Accession Version	XM_019970444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG 
CACTTTAAAG ARTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA 
CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC 
AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG 
GACTGCAGTG GCTCTCGCCC TCTRCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG 
CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCSGAGGT CACTCCTGCG 
CAGGCCAAGG CCTCRCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT 
GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT 
CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC 
CAGCCAGAGG AGACKGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG 
GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT 
GACATCCCTC TGCTCCCGRG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT 
TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC 
CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC 
AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA 
TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT 
CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA 
GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT 
CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826003.1
CDS	391..3861
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970444.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970444.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGGTCT CCAGAGAGAG CCCATTTCCT CTCCTGTAAT AACTGTAGAG 
CACTTTAAAG ARTCGACGGG TGTTAAAGGC CTTCCCCTAT ACCCGGACCC CTCCCGGGTA 
CCTGGCACGA AAACTCAGAA CAGTTTGGAA TCTGACTACT TGGCCCGAGA TGGCCCTTCC 
AGCAACAGCT CGTTCCACAG CAGCGAAGAG GAGGGGACCG ACCTCGAGGG GGACATGCTG 
GACTGCAGTG GCTCTCGCCC TCTRCTTTTG GAGTCAGAAG AAGAGGATGA GAGCTGCAAG 
CCTCCGCAGG GGAAGCTAGG AGGAGCTACT CCCTTCACTC AGCCSGAGGT CACTCCTGCG 
CAGGCCAAGG CCTCRCAAGG TGGGAGGAAG AACCAATTCG GAGCCCTCAT TCAGCCAATT 
GCAGATGGAC TCGGTGAGCC AGATGTGTTC GCCACTGCCC CCTTCCGGAG CTCAAGGATT 
CCCGCTGATG ACATTTTTGC CAAAGCCCCC TTTGTCTCCA AGGGCAACGT GGCCCCTTCC 
CAGCCAGAGG AGACKGATGT GTTCTTGAGA GCTCCCTTTA CCAAGAAGAA AGGCATGGAG 
GAGTCAACAG TTGCCCAGAG CCCCGCCCAG GAGCAGTCCG GTCTCCTCAG TCAGACCGAT 
GACATCCCTC TGCTCCCGRG CCTCGATCGA GCCGTGTACC CGTCTGCCCG AGCTCAGTAT 
TCCATGGCTG GCTTTGCCCA GCCGTCTAAC CTCCCCTCCC ATCCTGTACA AGCAGCAGAC 
CATCTTGACA GCATCTCTTC CAGGGGCTCC TCCCTGGAAT CTGGGGCCCA TCCTAATGAC 
AGAAACAAAG GACCTCAGCC CCAGAAAGAA GCTGTCTCAG GCCCCGTGGC AGGCAAACCA 
TTCCGCCCCC AGTCTTTATC CAAATATTCC CGTCACTATA GCCCGGAAGA TGAGCCGAAT 
CCAGAAGCCC AGCCCATCGC TGCCTACAAA ATTGTTTCGC AAACCAATAA GCAGTCGATA 
GCTGGCTCCG TTTCCATCAC ATCCCTTGCC TCCAGGACTA CGGAGCTGCC AGCTGCTGAT 
CCTTTTGCTT TAGCACCCTT TCCTTCGAAA TCAGGCAAAC AGAAACCTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970445.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826004.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBo29220
Clone ID Related Accession (Same CDS sequence)	XM_019970445.1
Accession Version	XM_019970445.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGARTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTRCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCSG AGGTCACTCC TGCGCAGGCC AAGGCCTCRC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACKG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGRGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826004.1
CDS	391..3765
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970445.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970445.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG GTCTCCAGAG AGAGCCCATT 
TCCTCTCCTG TAATAACTGT AGAGCACTTT AAAGARTCGA CGGGTGTTAA AGGCCTTCCC 
CTATACCCGG ACCCCTCCCG GGTACCTGGC ACGAAAACTC AGAACAGTTT GGAATCTGAC 
TACTTGGCCC GAGATGGCCC TTCCAGCAAC AGCTCGTTCC ACAGCAGCGA AGAGGAGGGG 
ACCGACCTCG AGGGGGACAT GCTGGACTGC AGTGGCTCTC GCCCTCTRCT TTTGGAGTCA 
GAAGAAGAGG ATGAGAGCTG CAAGCCTCCG CAGGGGAAGC TAGGAGGAGC TACTCCCTTC 
ACTCAGCCSG AGGTCACTCC TGCGCAGGCC AAGGCCTCRC AAGGTGGGAG GAAGAACCAA 
TTCGGAGCCC TCATTCAGCC AATTGCAGAT GGACTCGGTG AGCCAGATGT GTTCGCCACT 
GCCCCCTTCC GGAGCTCAAG GATTCCCGCT GATGACATTT TTGCCAAAGC CCCCTTTGTC 
TCCAAGGGCA ACGTGGCCCC TTCCCAGCCA GAGGAGACKG ATGTGTTCTT GAGAGCTCCC 
TTTACCAAGA AGAAAGGCAT GGAGGAGTCA ACAGTTGCCC AGAGCCCCGC CCAGGAGCAG 
TCCGGTCTCC TCAGTCAGAC CGATGACATC CCTCTGCTCC CGRGCCTCGA TCGAGCCGTG 
TACCCGTCTG CCCGAGCTCA GTATTCCATG GCTGGCTTTG CCCAGCCGTC TAACCTCCCC 
TCCCATCCTG TACAAGCAGC AGACCATCTT GACAGCATCT CTTCCAGGGG CTCCTCCCTG 
GAATCTGGGG CCCATCCTAA TGACAGAAAC AAAGGACCTC AGCCCCAGAA AGAAGCTGTC 
TCAGGCCCCG TGGCAGGCAA ACCATTCCGC CCCCAGTCTT TATCCAAATA TTCCCGTCAC 
TATAGCCCGG AAGATGAGCC GAATCCAGAA GCCCAGCCCA TCGCTGCCTA CAAAATTGTT 
TCGCAAACCA ATAAGCAGTC GATAGCTGGC TCCGTTTCCA TCACATCCCT TGCCTCCAGG 
ACTACGGAGC TGCCAGCTGC TGATCCTTTT GCTTTAGCAC CCTTTCCTTC GAAATCAGGC 
AAACAGAAAC CTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970446.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826005.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

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CloneID	OBo29221
Clone ID Related Accession (Same CDS sequence)	XM_019970446.1
Accession Version	XM_019970446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3270bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC 
CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA RTCGACGGGT 
GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC 
AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC 
AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT 
CTRCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA 
GGAGCTACTC CCTTCACTCA GCCSGAGGTC ACTCCTGCGC AGGCCAAGGC CTCRCAAGGT 
GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA 
GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC 
AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACKGATGTG 
TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC 
CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGRGC 
CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG 
CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC 
AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC 
CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC 
AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT 
GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA 
TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT 
CCTTCGAAAT CAGGCAAACA GAAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826005.1
CDS	391..3660
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGLQREPISSPVITVEHFKnullSTGVKGLPLYPDPSRVPGTKTQNSLESDYLARDGPSSNSSFHSSEEEGTDLEGDMLDCSGSRPnullLLESEEEDESCKPPQGKLGGATPFTQnullEVTPAQAKAnullQGGRKNQFGALIQPIADGLGEPDVFATAPFRSSRIPADDIFAKAPFVSKGNVAPSQPEEnullDVFLRAPFTKKKGMEESTVAQSPAQEQSGLLSQTDDIPLLPnullLDRAVYPSARAQYSMAGFAQPSNLPSHPVQAADHLDSISSRGSSLESGAHPNDRNKGPQPQKEAVSGPVAGKPFRPQSLSKYSRHYSPEDEPNPEAQPIAAYKIVSQTNKQSIAGSVSITSLASRTTELPAADPFALAPFPSKSGKQKP

Target ORF information:

RefSeq Version	XM_019970446.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970446.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGTCTC 
CAGAGAGAGC CCATTTCCTC TCCTGTAATA ACTGTAGAGC ACTTTAAAGA RTCGACGGGT 
GTTAAAGGCC TTCCCCTATA CCCGGACCCC TCCCGGGTAC CTGGCACGAA AACTCAGAAC 
AGTTTGGAAT CTGACTACTT GGCCCGAGAT GGCCCTTCCA GCAACAGCTC GTTCCACAGC 
AGCGAAGAGG AGGGGACCGA CCTCGAGGGG GACATGCTGG ACTGCAGTGG CTCTCGCCCT 
CTRCTTTTGG AGTCAGAAGA AGAGGATGAG AGCTGCAAGC CTCCGCAGGG GAAGCTAGGA 
GGAGCTACTC CCTTCACTCA GCCSGAGGTC ACTCCTGCGC AGGCCAAGGC CTCRCAAGGT 
GGGAGGAAGA ACCAATTCGG AGCCCTCATT CAGCCAATTG CAGATGGACT CGGTGAGCCA 
GATGTGTTCG CCACTGCCCC CTTCCGGAGC TCAAGGATTC CCGCTGATGA CATTTTTGCC 
AAAGCCCCCT TTGTCTCCAA GGGCAACGTG GCCCCTTCCC AGCCAGAGGA GACKGATGTG 
TTCTTGAGAG CTCCCTTTAC CAAGAAGAAA GGCATGGAGG AGTCAACAGT TGCCCAGAGC 
CCCGCCCAGG AGCAGTCCGG TCTCCTCAGT CAGACCGATG ACATCCCTCT GCTCCCGRGC 
CTCGATCGAG CCGTGTACCC GTCTGCCCGA GCTCAGTATT CCATGGCTGG CTTTGCCCAG 
CCGTCTAACC TCCCCTCCCA TCCTGTACAA GCAGCAGACC ATCTTGACAG CATCTCTTCC 
AGGGGCTCCT CCCTGGAATC TGGGGCCCAT CCTAATGACA GAAACAAAGG ACCTCAGCCC 
CAGAAAGAAG CTGTCTCAGG CCCCGTGGCA GGCAAACCAT TCCGCCCCCA GTCTTTATCC 
AAATATTCCC GTCACTATAG CCCGGAAGAT GAGCCGAATC CAGAAGCCCA GCCCATCGCT 
GCCTACAAAA TTGTTTCGCA AACCAATAAG CAGTCGATAG CTGGCTCCGT TTCCATCACA 
TCCCTTGCCT CCAGGACTAC GGAGCTGCCA GCTGCTGATC CTTTTGCTTT AGCACCCTTT 
CCTTCGAAAT CAGGCAAACA GAAACCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970447.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826006.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBo29222
Clone ID Related Accession (Same CDS sequence)	XM_019970447.1
Accession Version	XM_019970447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTYCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC 
AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826006.1
CDS	395..3271
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDnullLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_019970447.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970447.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG ATTCTCTCAC CGGGGAAGAT 
TCCCTGATTG ATTGCTCTCT GCTTTCTAAC CCCACTACTG ACCTYCTGGA AGAGTTTGCC 
CCCATAGCCA TCTCTGCTCC GGCCCATAAA GCTGCGGAAG ATAGTAATCT CATCTCAGGT 
TTTGATGTCC CTGAGGGCTC GGACAAGGTG GCAGAAGATG AGTTTGACCC TATTCCTGTA 
TTGATAACCA AAAACCCACA AGGTGGGCAC TCTAGAAACA GCAGTGGGAG CTCTGAGTCC 
AGTCTTCCCA ACCTAGCCAG GTCTTTGCTG CTGGTGGATC AGCTCATAGA CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970448.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826007.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBo29223
Clone ID Related Accession (Same CDS sequence)	XM_019970448.1
Accession Version	XM_019970448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TYCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT 
CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826007.1
CDS	394..3267
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTDSLTGEDSLIDCSLLSNPTTDnullLEEFAPIAISAPAHKAAEDSNLISGFDVPEGSDKVAEDEFDPIPVLITKNPQGGHSRNSSGSSESSLPNLARSLLLVDQLIDL

Target ORF information:

RefSeq Version	XM_019970448.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970448.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGATTCAA GCCCCAGTAA GACAACAGCC AAAGGTTCAG 
ACAACCCCAC CTCCTACCAT CCAGGGGCAG AAACTCGGAT CTCTCACTCC TCCATCATCC 
CCCAAAGCCC AGCGTGCTGG GCACAGGCGA ATTCTCAGCG ACGTGACCCA CAGCGCAGTC 
TTTGGGGTCC CTGCCAGCAA ATCAACCCAG CTGCTCCAAG CAGCTGCAGC TGAGGCCAGT 
CTCAATAAGT CCAAGTCTGC AACCACCACT CCTTCAGGCT CCCCTCGGGC CTCCCAGCAA 
AATGTTTATA ATCCTTCAGA AGGTTCTACC TGGAATCCCT TTGATGACGA CAATTTCTCC 
AAACTGACAG CTGAAGAACT GCTAAACAAG GACTTTGCCA AGCTGGGGGA AGGCAAGTAT 
CCTGAGAAGC TTGGAGGCTC TGCTGAGAGT TTGATCCCAG GCTTTCAACC AACCCAAGGT 
GATGCTTTTG CTGCTTCCTC ATTTTCTGCT GGAACTGCTG AAAAAAGGAA GGGTGGGCAG 
ACTATGGATT CTAGCCTTCC TCTTCTAAGT GTATCTGATC CTTTCATTCC TCTTCAAGTA 
CCTGATGCAC CAGAAAAACT AATTGAGGGA CTCAAATCTC CTGACACTTC TCTTCTGCTC 
CCTGACCTCT TGCCTATGAC AGATCCTTTT GGTAGCACTT CTGATGCTGT AATTGAAAAA 
GCTGATGTTG CTGTTGAGAG TCTGATACCA GGACTGGAGC CCCCGGTGCC TCAGCGCCTT 
CCATCTCAGA CGGAATCTGT GACCTCGAAC CGCACAGATT CTCTCACCGG GGAAGATTCC 
CTGATTGATT GCTCTCTGCT TTCTAACCCC ACTACTGACC TYCTGGAAGA GTTTGCCCCC 
ATAGCCATCT CTGCTCCGGC CCATAAAGCT GCGGAAGATA GTAATCTCAT CTCAGGTTTT 
GATGTCCCTG AGGGCTCGGA CAAGGTGGCA GAAGATGAGT TTGACCCTAT TCCTGTATTG 
ATAACCAAAA ACCCACAAGG TGGGCACTCT AGAAACAGCA GTGGGAGCTC TGAGTCCAGT 
CTTCCCAACC TAGCCAGGTC TTTGCTGCTG GTGGATCAGC TCATAGACCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AAK1 ( XM_019970449.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019826008.1 Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

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CloneID	OBo29224
Clone ID Related Accession (Same CDS sequence)	XM_019970449.1
Accession Version	XM_019970449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2571bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	AP2-associated protein kinase 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032660.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019826008.1
CDS	393..2963
Translation	MMKKFFDSRREQGGSGLGSGSSGGGGSTSGLGSGYIGRVFGIGRQQVTVDEVLAEGGFAIVFLVRTSNGMKCALKRMFVNNEHDLQVCKREIQIMRDLSGHKNIVGYIDSSINSVSSGDVWEVLILMDFCRGGQVVNLMNQRLQTGFTENEVLQIFCDTCEAnullARLHQCKTPIIHRDLKVENILLHDRGHYVLCDFGSATNKFQNPQTEGVNAVEDEIKKYTTLSYRAPEMVNLYSGKVITTKADIWALGCLLYKLCYFTLPFGESQVAICDGNFTIPDNSRYSQDMHCLIRYMLEPDPDKRPDIYQVSYFSFKLLKKECnullIPNVQNSPIPTKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASVPQPKTQPPPSQPLPQSQPKQPQAPPTSQQPPSAPAQALPTQAQATPQHQQQLFLKQQQQQQTAPPAQQPAGnullFYQQPQQQAQAPQFQAVHPAAQQPVIAQFPVVSQGSSQQQLIQNFYQQQQQQQQLATALHQQQLLTQQAALQQKTTAAAAPQPQAQPAAAASPAPAQEPAQIQAPVRQQPKVQTTPPPTIQGQKLGSLTPPSSPKAQRAGHRRILSDVTHSAVFGVPASKSTQLLQAAAAEASLNKSKSATTTPSGSPRASQQNVYNPSEGSTWNPFDDDNFSKLTAEELLNKDFAKLGEGKYPEKLGGSAESLIPGFQPTQGDAFAASSFSAGTAEKRKGGQTMDSSLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIEKADVAVESLIPGLEPPVPQRLPSQTESVTSNRTAKT

Target ORF information:

RefSeq Version	XM_019970449.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus AP2 associated kinase 1 (AAK1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019970449.1

ORF Insert Sequence:

ATGATGAAGA AGTTTTTCGA CTCCAGGCGA GAGCAGGGTG GCTCTGGCCT GGGCTCCGGC 
TCCAGCGGAG GAGGGGGCAG CACCTCGGGC CTGGGCAGCG GCTACATCGG AAGGGTCTTT 
GGCATTGGGC GACAGCAGGT CACTGTGGAC GAGGTGCTGG CGGAAGGTGG ATTTGCCATC 
GTGTTTCTGG TGAGGACGAG CAATGGAATG AAATGTGCCT TGAAACGCAT GTTTGTCAAC 
AACGAGCATG ATCTCCAGGT GTGCAAGAGA GAAATCCAGA TAATGAGGGA CCTGTCGGGG 
CATAAGAACA TCGTGGGTTA CATTGACTCC AGTATCAATA GTGTGAGTAG CGGTGATGTG 
TGGGAAGTTC TCATCCTGAT GGACTTCTGC AGGGGAGGCC AAGTGGTCAA CCTAATGAAC 
CAGCGCCTGC AAACAGGTTT TACAGAAAAT GAAGTACTCC AGATATTTTG TGACACCTGT 
GAAGCTGTYG CCCGCCTGCA TCAGTGCAAA ACTCCTATTA TCCACCGTGA CCTGAAGGTT 
GAGAATATTC TCTTGCATGA CCGAGGCCAC TATGTCCTGT GTGACTTTGG AAGTGCTACC 
AACAAATTCC AGAATCCACA AACAGAAGGA GTCAATGCAG TAGAAGATGA GATTAAGAAG 
TACACAACGC TGTCCTATCG CGCACCAGAG ATGGTCAACC TGTACAGTGG CAAGGTCATC 
ACGACGAAGG CAGACATTTG GGCTCTCGGA TGTTTGTTGT ATAAATTATG TTACTTCACT 
TTGCCATTTG GGGAGAGTCA AGTGGCAATT TGTGATGGAA ACTTCACAAT TCCTGATAAC 
TCTCGATATT CCCAAGACAT GCACTGCCTA ATTAGGTACA TGTTGGAACC AGACCCTGAC 
AAAAGGCCGG ATATTTACCA GGTTTCCTAC TTCTCATTTA AACTACTCAA GAAAGAATGC 
CCWATTCCAA ATGTACAGAA CTCTCCCATT CCTACAAAGC TCCCTGAACC AGTGAAAGCC 
AGTGAGGCAG CTGCAAAGAA GACCCAGCCA AAGGCCAGAC TGACAGATCC CATTCCCACT 
ACAGAGACGT CAATTGCACC CCGCCAGAGG CCTAAAGCTG GGCAGACTCA GCCCAACCCA 
GGAATCCTCC CCATCCAACC AGCCCTGACC CCTCGGAAGA GGGCCACAGT TCAGCCCCCG 
CCTCAGGCCG CAGGATCCAG CAATCAGCCT GGTCTTTTAG CCAGTGTTCC TCAACCAAAA 
ACCCAGCCCC CACCCAGTCA ACCCCTACCA CAGTCTCAGC CCAAGCAGCC TCAGGCTCCG 
CCCACCTCAC AGCAGCCGCC TTCCGCGCCG GCCCAGGCTC TGCCCACCCA GGCCCAGGCC 
ACGCCCCAGC ACCAGCAGCA ACTCTTCCTC AAGCAGCAGC AGCAGCAGCA GACAGCGCCG 
CCCGCACAGC AGCCAGCGGG CACKTTCTAC CAGCAGCCAC AACAGCAGGC CCAGGCTCCA 
CAGTTTCAGG CAGTACACCC AGCAGCCCAG CAACCAGTGA TCGCTCAGTT CCCCGTGGTA 
TCTCAAGGAA GCTCTCAACA GCAGCTGATA CAGAACTTCT ACCAGCAGCA GCAGCAGCAG 
CAGCAGCTGG CCACAGCCCT GCATCAGCAA CAGCTGCTGA CTCAGCAGGC TGCCTTGCAG 
CAGAAGACGA CAGCAGCGGC CGCACCGCAG CCCCAAGCAC AGCCGGCCGC AGCAGCCTCG 
CCAGCCCCTG CCCAGGAGCC AGCGCAGATT CAAGCCCCAG TAAGACAACA GCCAAAGGTT 
CAGACAACCC CACCTCCTAC CATCCAGGGG CAGAAACTCG GATCTCTCAC TCCTCCATCA 
TCCCCCAAAG CCCAGCGTGC TGGGCACAGG CGAATTCTCA GCGACGTGAC CCACAGCGCA 
GTCTTTGGGG TCCCTGCCAG CAAATCAACC CAGCTGCTCC AAGCAGCTGC AGCTGAGGCC 
AGTCTCAATA AGTCCAAGTC TGCAACCACC ACTCCTTCAG GCTCCCCTCG GGCCTCCCAG 
CAAAATGTTT ATAATCCTTC AGAAGGTTCT ACCTGGAATC CCTTTGATGA CGACAATTTC 
TCCAAACTGA CAGCTGAAGA ACTGCTAAAC AAGGACTTTG CCAAGCTGGG GGAAGGCAAG 
TATCCTGAGA AGCTTGGAGG CTCTGCTGAG AGTTTGATCC CAGGCTTTCA ACCAACCCAA 
GGTGATGCTT TTGCTGCTTC CTCATTTTCT GCTGGAACTG CTGAAAAAAG GAAGGGTGGG 
CAGACTATGG ATTCTAGCCT TCCTCTTCTA AGTGTATCTG ATCCTTTCAT TCCTCTTCAA 
GTACCTGATG CACCAGAAAA ACTAATTGAG GGACTCAAAT CTCCTGACAC TTCTCTTCTG 
CTCCCTGACC TCTTGCCTAT GACAGATCCT TTTGGTAGCA CTTCTGATGC TGTAATTGAA 
AAAGCTGATG TTGCTGTTGA GAGTCTGATA CCAGGACTGG AGCCCCCGGT GCCTCAGCGC 
CTTCCATCTC AGACGGAATC TGTGACCTCG AACCGCACAG CTAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	AAK1
Entrez Gene ID	109566238
Full Name	AP2 associated kinase 1
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

AAK1 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene

Clones

gRNAs

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