SLC12A6 cDNA ORF clone, Bos indicus(Bos taurus indicus)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A6
Entrez Gene ID	109564891
Full Name	solute carrier family 12 member 6
Gene Type	protein-coding
Organism	Bos indicus(Bos taurus indicus)

mRNA and Protein(s)

mRNA	Protein	Name
XM_019968460.1	XP_019824019.1	solute carrier family 12 member 6 isoform X2
XM_019968459.1	XP_019824018.1	solute carrier family 12 member 6 isoform X1

Pathways from BioSystems
Gene Ontology

The following SLC12A6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBo27722	XM_019968459.1 Latest version!	Bos indicus solute carrier family 12 member 6 (SLC12A6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBo27723	XM_019968460.1 Latest version!	Bos indicus solute carrier family 12 member 6 (SLC12A6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A6 ( XM_019968459.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019824018.1 Bos indicus solute carrier family 12 member 6 (SLC12A6), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo27722
Clone ID Related Accession (Same CDS sequence)	XM_019968459.1
Accession Version	XM_019968459.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	solute carrier family 12 member 6 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032659.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT 
TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG 
AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC 
CGGACATCTA ACCCCCAGGA TGTTACTGAG GACCCGAGTC AGAACTCCAT CACAGGGGAA 
CACAGCCAGC TGTTAGATGA TGGACATAAG AAAGCGCGAA ATGCTTATCT TAACAATTCC 
AATTATGAAG AAGGAGATGA ATATTTTGAT AAGAATTTGG CACTCTTTGA GGAAGAAATG 
GACACCAGAC CAAAGGTGTC TTCTCTCCTT AGCCGCTTGG CCAATTATAC TAATCTGACA 
CAAGGAGCAA AGGAACATGA AGAGGCAGAG AACATCACTG AAGGGAAAAA GAAGCCCACC 
AAGACTCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT 
GGAGTGATCC TGTTTCTGCG CCTCACATGG GTGGTGGGCA CAGCTGGAGT TCTGCAGGCC 
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACRATGTTGA CTGCTATCTC CATGAGTGCC 
ATTGCCACTA ATGGAGTGGT GCCAGCTGGA GGCTCATACT TCATGATTTC CCGAGCACTG 
GGCCCGGAGT TTGGTGGAGC TGTTGGCCTC TGCTTCTATC TTGGTACCAC ATTTGCAGCA 
GCTATGTATA TCCTTGGTGC CATTGAAATA TTTCTGGTAT ATATCGTCCC TCGAGCTGCC 
ATCTTTCAGA GTGACGATGC ACTCAAGGAG TCAGCAGCCA TGCTAAATAA CATGCGTGTC 
TATGGGACAG CCTTCTTGGT CCTCATGGTA TTGGTGGTAT TCATCGGCGT ACGCTATGTG 
AACAAGTTTG CCTCACTCTT TCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTACGCT 
GGAGCCATCA AGTCTTCTTT CGCACCTCCA CACTTCCCGG TCTGCATGCT GGGCAACCGC 
ACCCTTTCAT CAAGACATAT TGACGTTTGC TCAAAGACCA AGGAAGTTAA CAACATGACA 
GTGCCGTCAA AGCTGTGGGG CTTCTTCTGT AACTCAAGTC AGTTTTTCAA CGCCACTTGC 
GATGAGTACT TTGTTCACAA TAATGTCACT TCGATCCAGG GCATCCCTGG ATTGGCTAGC 
GGTGTCATTA CAGAGAATCT TTGGAGTAAT TATCTACCAA AGGGAGAGAT CATTGAGAAG 
CCCTCAGCCA AATCTTCCGA AGTCTTAGGC AGCTTAAACC ATGAGTATGT CCTTGTGGAC 
ATCACCACCT CTTTCACTCT TCTGGTGGGC ATCTTCTTTC CCTCTGTCAC AGGCATCATG 
GCAGGATCAA ACAGATCTGG GGATCTGAAA GATGCTCAAA AGTCCATTCC CATTGGCACT 
ATCCTCGCCA TCCTGACCAC CTCCTTTGTC TATTTAAGCA ACGTTGTCCT TTTTGGTGCA 
TGTATTGAAG GTGTTGTCCT CAGAGACAAG TTTGGAGATG CTGTGAAAGG TAATCTGGTG 
GTAGGAACCT TATCTTGGCC ATCCCCGTGG GTGATTGTTA TTGGCTCCTT CTTCTCCACA 
TGTGGGGCTG GACTTCAGAG TCTCACAGGA GCACCGAGGC TGCTACAGGC TATAGCCAAG 
GATAACATTA TACCCTTTCT GAGGGTTTTT GGTCACAGCA AAGCCAATGG GGAACCTACC 
TGGGCTTTAC TTCTGACTGC TGCCATTGCA GAACTGGGAA TCCTTATTGC CTCCCTGGAT 
CTCGTGGCTC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT GAATTTGGCA 
TGTGCCTTGC AAACATTGCT TCGAACACCC AACTGGAGAC CCCGGTTCCG CTACTACCAC 
TGGGCACTCT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG 
TATTATGCCA TCGTAGCTAT GGTAATAGCT GGTATGATCT ACAAATACAT CGAGTACCAA 
GGAGCTGAGA AAGAATGGGG TGATGGTATC CGCGGGCTGT CCCTCAGTGC AGCCCGATTT 
GCTTTGCTTA GGCTGGAGGA AGGACCACCA CACACTAAAA ACTGGAGGCC TCAGTTACTT 
GTATTGCTGA AACTAGATGA AGACCTACAC GTCAAGCATC CTCGTCTCCT CACCTTTGCT 
TCACAGCTTA AGGCAGGAAA GGGTCTCACA ATTGTGGGCT CTGTCATCGT CGGGAACTTC 
CTGGAGAACT ATGGTGAAGC GTTAGCTGCT GAGCAGACCA TAAAGCATTT AATGGAGGCA 
GAGAAGGTGA AAGGGTTCTC ACAGCTGGTG GTGGCCGCCA AACTGAGGGA GGGCATTTCC 
CACCTCATCC AGTCGTGTGG CCTTGGGGGC ATGAAGCACA ACACAGTGGT GATGGGATGG 
CCAAATGGCT GGCGCCAGAG TGAAGATGCT CGAGCTTGGA AGACTTTTAT TGGCACAGTT 
CGAGTGACAA CTGCTGCCCA TCTGGCCCTG CTGGTGGCCA AAAACATCTC CTTCTTTCCG 
AGCAATGTGG AGCAGTTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT ACATGATGGG 
GGGATGCTCA TGCTATTACC ATTCCTACTG AAACAGCATA AGGTGTGGCG AAAATGCAGC 
ATACGGATCT TCACAGTAGC CCAGCTAGAA GACAACAGTA TTCAGATGAA GAAGGACCTG 
GCCACCTTCT TGTATCACCT TCGCATTGAG GCAGAGGTGG AAGTGGTAGA GATGCATGAC 
AGTGACATAT CCGCATACAC TTACGAGCGC ACCCTGATGA TGGAGCAGAG GTCACAGATG 
CTGCGGCACA TGCGCCTGTC CAAGACGGAG CGAGACCGAG AGGCACAGCT GGTGAAAGAT 
CGGAACTCAA TGCTACGACT GACCAGCATC GGCTCTGACG AGGATGACGA GACAGAGACT 
TATCAGGAGA AGGTGCACAT GACGTGGACG AAAGACAAGT ATATGGCGTC TCGGGGACAA 
AAGGCCAAGT CAATGGAAGG GTTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT 
GTGAGGCGGA TGCATACAGC AGTGAAGCTC AATGAGGTTA TAGTTAACAA GTCCCATGAA 
GCAAAGCTGG TTTTGTTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGACGAAAAC 
TACATGGAGT TCCTGGAAGT ACTCACTGAA GGACTAGAGC GAGTCCTTCT TGTCCGAGGT 
GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019824018.1
CDS	1217..4669
Translation	MHPPETTTKMASVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSEMSGATTSLATVALDQASDRTSNPQDVTEDPSQNSITGEHSQLLDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLSRLANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCnullMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFQSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEVNNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGVITENLWSNYLPKGEIIEKPSAKSSEVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFSQLVVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDDETETYQEKVHMTWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	XM_019968459.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus solute carrier family 12 member 6 (SLC12A6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019968459.1

ORF Insert Sequence:

ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT 
TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG 
AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC 
CGGACATCTA ACCCCCAGGA TGTTACTGAG GACCCGAGTC AGAACTCCAT CACAGGGGAA 
CACAGCCAGC TGTTAGATGA TGGACATAAG AAAGCGCGAA ATGCTTATCT TAACAATTCC 
AATTATGAAG AAGGAGATGA ATATTTTGAT AAGAATTTGG CACTCTTTGA GGAAGAAATG 
GACACCAGAC CAAAGGTGTC TTCTCTCCTT AGCCGCTTGG CCAATTATAC TAATCTGACA 
CAAGGAGCAA AGGAACATGA AGAGGCAGAG AACATCACTG AAGGGAAAAA GAAGCCCACC 
AAGACTCCCC AAATGGGTAC CTTCATGGGT GTCTACCTCC CATGTCTACA AAATATTTTT 
GGAGTGATCC TGTTTCTGCG CCTCACATGG GTGGTGGGCA CAGCTGGAGT TCTGCAGGCC 
TTTGCAATTG TCCTTATCTG CTGCTGCTGT ACRATGTTGA CTGCTATCTC CATGAGTGCC 
ATTGCCACTA ATGGAGTGGT GCCAGCTGGA GGCTCATACT TCATGATTTC CCGAGCACTG 
GGCCCGGAGT TTGGTGGAGC TGTTGGCCTC TGCTTCTATC TTGGTACCAC ATTTGCAGCA 
GCTATGTATA TCCTTGGTGC CATTGAAATA TTTCTGGTAT ATATCGTCCC TCGAGCTGCC 
ATCTTTCAGA GTGACGATGC ACTCAAGGAG TCAGCAGCCA TGCTAAATAA CATGCGTGTC 
TATGGGACAG CCTTCTTGGT CCTCATGGTA TTGGTGGTAT TCATCGGCGT ACGCTATGTG 
AACAAGTTTG CCTCACTCTT TCTGGCCTGT GTCATTGTGT CCATCTTGGC CATCTACGCT 
GGAGCCATCA AGTCTTCTTT CGCACCTCCA CACTTCCCGG TCTGCATGCT GGGCAACCGC 
ACCCTTTCAT CAAGACATAT TGACGTTTGC TCAAAGACCA AGGAAGTTAA CAACATGACA 
GTGCCGTCAA AGCTGTGGGG CTTCTTCTGT AACTCAAGTC AGTTTTTCAA CGCCACTTGC 
GATGAGTACT TTGTTCACAA TAATGTCACT TCGATCCAGG GCATCCCTGG ATTGGCTAGC 
GGTGTCATTA CAGAGAATCT TTGGAGTAAT TATCTACCAA AGGGAGAGAT CATTGAGAAG 
CCCTCAGCCA AATCTTCCGA AGTCTTAGGC AGCTTAAACC ATGAGTATGT CCTTGTGGAC 
ATCACCACCT CTTTCACTCT TCTGGTGGGC ATCTTCTTTC CCTCTGTCAC AGGCATCATG 
GCAGGATCAA ACAGATCTGG GGATCTGAAA GATGCTCAAA AGTCCATTCC CATTGGCACT 
ATCCTCGCCA TCCTGACCAC CTCCTTTGTC TATTTAAGCA ACGTTGTCCT TTTTGGTGCA 
TGTATTGAAG GTGTTGTCCT CAGAGACAAG TTTGGAGATG CTGTGAAAGG TAATCTGGTG 
GTAGGAACCT TATCTTGGCC ATCCCCGTGG GTGATTGTTA TTGGCTCCTT CTTCTCCACA 
TGTGGGGCTG GACTTCAGAG TCTCACAGGA GCACCGAGGC TGCTACAGGC TATAGCCAAG 
GATAACATTA TACCCTTTCT GAGGGTTTTT GGTCACAGCA AAGCCAATGG GGAACCTACC 
TGGGCTTTAC TTCTGACTGC TGCCATTGCA GAACTGGGAA TCCTTATTGC CTCCCTGGAT 
CTCGTGGCTC CAATTCTTTC CATGTTTTTT CTCATGTGTT ACCTCTTTGT GAATTTGGCA 
TGTGCCTTGC AAACATTGCT TCGAACACCC AACTGGAGAC CCCGGTTCCG CTACTACCAC 
TGGGCACTCT CTTTCATGGG AATGAGTATC TGTCTGGCTC TGATGTTCAT TTCTTCCTGG 
TATTATGCCA TCGTAGCTAT GGTAATAGCT GGTATGATCT ACAAATACAT CGAGTACCAA 
GGAGCTGAGA AAGAATGGGG TGATGGTATC CGCGGGCTGT CCCTCAGTGC AGCCCGATTT 
GCTTTGCTTA GGCTGGAGGA AGGACCACCA CACACTAAAA ACTGGAGGCC TCAGTTACTT 
GTATTGCTGA AACTAGATGA AGACCTACAC GTCAAGCATC CTCGTCTCCT CACCTTTGCT 
TCACAGCTTA AGGCAGGAAA GGGTCTCACA ATTGTGGGCT CTGTCATCGT CGGGAACTTC 
CTGGAGAACT ATGGTGAAGC GTTAGCTGCT GAGCAGACCA TAAAGCATTT AATGGAGGCA 
GAGAAGGTGA AAGGGTTCTC ACAGCTGGTG GTGGCCGCCA AACTGAGGGA GGGCATTTCC 
CACCTCATCC AGTCGTGTGG CCTTGGGGGC ATGAAGCACA ACACAGTGGT GATGGGATGG 
CCAAATGGCT GGCGCCAGAG TGAAGATGCT CGAGCTTGGA AGACTTTTAT TGGCACAGTT 
CGAGTGACAA CTGCTGCCCA TCTGGCCCTG CTGGTGGCCA AAAACATCTC CTTCTTTCCG 
AGCAATGTGG AGCAGTTTTC TGAGGGCAAC ATTGATGTGT GGTGGATTGT ACATGATGGG 
GGGATGCTCA TGCTATTACC ATTCCTACTG AAACAGCATA AGGTGTGGCG AAAATGCAGC 
ATACGGATCT TCACAGTAGC CCAGCTAGAA GACAACAGTA TTCAGATGAA GAAGGACCTG 
GCCACCTTCT TGTATCACCT TCGCATTGAG GCAGAGGTGG AAGTGGTAGA GATGCATGAC 
AGTGACATAT CCGCATACAC TTACGAGCGC ACCCTGATGA TGGAGCAGAG GTCACAGATG 
CTGCGGCACA TGCGCCTGTC CAAGACGGAG CGAGACCGAG AGGCACAGCT GGTGAAAGAT 
CGGAACTCAA TGCTACGACT GACCAGCATC GGCTCTGACG AGGATGACGA GACAGAGACT 
TATCAGGAGA AGGTGCACAT GACGTGGACG AAAGACAAGT ATATGGCGTC TCGGGGACAA 
AAGGCCAAGT CAATGGAAGG GTTCCAGGAC CTGCTTAACA TGCGTCCGGA CCAGTCCAAT 
GTGAGGCGGA TGCATACAGC AGTGAAGCTC AATGAGGTTA TAGTTAACAA GTCCCATGAA 
GCAAAGCTGG TTTTGTTGAA TATGCCAGGG CCACCCCGAA ACCCTGAGGG TGACGAAAAC 
TACATGGAGT TCCTGGAAGT ACTCACTGAA GGACTAGAGC GAGTCCTTCT TGTCCGAGGT 
GGTGGCAGTG AAGTGATCAC CATTTATTCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A6 ( XM_019968460.1 ) cDNA ORF clone, Bos indicus(Bos taurus indicus) -> XP_019824019.1 Bos indicus solute carrier family 12 member 6 (SLC12A6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo27723
Clone ID Related Accession (Same CDS sequence)	XM_019968460.1
Accession Version	XM_019968460.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-11
Organism	Bos indicus(Bos taurus indicus)
Product	solute carrier family 12 member 6 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_032659.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos indicus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT 
TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG 
AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC 
CGGACATCTA ACCCCCAGGA TGTTACTGAG GATGATGGAC ATAAGAAAGC GCGAAATGCT 
TATCTTAACA ATTCCAATTA TGAAGAAGGA GATGAATATT TTGATAAGAA TTTGGCACTC 
TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTTAGCCG CTTGGCCAAT 
TATACTAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG 
AAAAAGAAGC CCACCAAGAC TCCCCAAATG GGTACCTTCA TGGGTGTCTA CCTCCCATGT 
CTACAAAATA TTTTTGGAGT GATCCTGTTT CTGCGCCTCA CATGGGTGGT GGGCACAGCT 
GGAGTTCTGC AGGCCTTTGC AATTGTCCTT ATCTGCTGCT GCTGTACRAT GTTGACTGCT 
ATCTCCATGA GTGCCATTGC CACTAATGGA GTGGTGCCAG CTGGAGGCTC ATACTTCATG 
ATTTCCCGAG CACTGGGCCC GGAGTTTGGT GGAGCTGTTG GCCTCTGCTT CTATCTTGGT 
ACCACATTTG CAGCAGCTAT GTATATCCTT GGTGCCATTG AAATATTTCT GGTATATATC 
GTCCCTCGAG CTGCCATCTT TCAGAGTGAC GATGCACTCA AGGAGTCAGC AGCCATGCTA 
AATAACATGC GTGTCTATGG GACAGCCTTC TTGGTCCTCA TGGTATTGGT GGTATTCATC 
GGCGTACGCT ATGTGAACAA GTTTGCCTCA CTCTTTCTGG CCTGTGTCAT TGTGTCCATC 
TTGGCCATCT ACGCTGGAGC CATCAAGTCT TCTTTCGCAC CTCCACACTT CCCGGTCTGC 
ATGCTGGGCA ACCGCACCCT TTCATCAAGA CATATTGACG TTTGCTCAAA GACCAAGGAA 
GTTAACAACA TGACAGTGCC GTCAAAGCTG TGGGGCTTCT TCTGTAACTC AAGTCAGTTT 
TTCAACGCCA CTTGCGATGA GTACTTTGTT CACAATAATG TCACTTCGAT CCAGGGCATC 
CCTGGATTGG CTAGCGGTGT CATTACAGAG AATCTTTGGA GTAATTATCT ACCAAAGGGA 
GAGATCATTG AGAAGCCCTC AGCCAAATCT TCCGAAGTCT TAGGCAGCTT AAACCATGAG 
TATGTCCTTG TGGACATCAC CACCTCTTTC ACTCTTCTGG TGGGCATCTT CTTTCCCTCT 
GTCACAGGCA TCATGGCAGG ATCAAACAGA TCTGGGGATC TGAAAGATGC TCAAAAGTCC 
ATTCCCATTG GCACTATCCT CGCCATCCTG ACCACCTCCT TTGTCTATTT AAGCAACGTT 
GTCCTTTTTG GTGCATGTAT TGAAGGTGTT GTCCTCAGAG ACAAGTTTGG AGATGCTGTG 
AAAGGTAATC TGGTGGTAGG AACCTTATCT TGGCCATCCC CGTGGGTGAT TGTTATTGGC 
TCCTTCTTCT CCACATGTGG GGCTGGACTT CAGAGTCTCA CAGGAGCACC GAGGCTGCTA 
CAGGCTATAG CCAAGGATAA CATTATACCC TTTCTGAGGG TTTTTGGTCA CAGCAAAGCC 
AATGGGGAAC CTACCTGGGC TTTACTTCTG ACTGCTGCCA TTGCAGAACT GGGAATCCTT 
ATTGCCTCCC TGGATCTCGT GGCTCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC 
TTTGTGAATT TGGCATGTGC CTTGCAAACA TTGCTTCGAA CACCCAACTG GAGACCCCGG 
TTCCGCTACT ACCACTGGGC ACTCTCTTTC ATGGGAATGA GTATCTGTCT GGCTCTGATG 
TTCATTTCTT CCTGGTATTA TGCCATCGTA GCTATGGTAA TAGCTGGTAT GATCTACAAA 
TACATCGAGT ACCAAGGAGC TGAGAAAGAA TGGGGTGATG GTATCCGCGG GCTGTCCCTC 
AGTGCAGCCC GATTTGCTTT GCTTAGGCTG GAGGAAGGAC CACCACACAC TAAAAACTGG 
AGGCCTCAGT TACTTGTATT GCTGAAACTA GATGAAGACC TACACGTCAA GCATCCTCGT 
CTCCTCACCT TTGCTTCACA GCTTAAGGCA GGAAAGGGTC TCACAATTGT GGGCTCTGTC 
ATCGTCGGGA ACTTCCTGGA GAACTATGGT GAAGCGTTAG CTGCTGAGCA GACCATAAAG 
CATTTAATGG AGGCAGAGAA GGTGAAAGGG TTCTCACAGC TGGTGGTGGC CGCCAAACTG 
AGGGAGGGCA TTTCCCACCT CATCCAGTCG TGTGGCCTTG GGGGCATGAA GCACAACACA 
GTGGTGATGG GATGGCCAAA TGGCTGGCGC CAGAGTGAAG ATGCTCGAGC TTGGAAGACT 
TTTATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTGG CCCTGCTGGT GGCCAAAAAC 
ATCTCCTTCT TTCCGAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG 
ATTGTACATG ATGGGGGGAT GCTCATGCTA TTACCATTCC TACTGAAACA GCATAAGGTG 
TGGCGAAAAT GCAGCATACG GATCTTCACA GTAGCCCAGC TAGAAGACAA CAGTATTCAG 
ATGAAGAAGG ACCTGGCCAC CTTCTTGTAT CACCTTCGCA TTGAGGCAGA GGTGGAAGTG 
GTAGAGATGC ATGACAGTGA CATATCCGCA TACACTTACG AGCGCACCCT GATGATGGAG 
CAGAGGTCAC AGATGCTGCG GCACATGCGC CTGTCCAAGA CGGAGCGAGA CCGAGAGGCA 
CAGCTGGTGA AAGATCGGAA CTCAATGCTA CGACTGACCA GCATCGGCTC TGACGAGGAT 
GACGAGACAG AGACTTATCA GGAGAAGGTG CACATGACGT GGACGAAAGA CAAGTATATG 
GCGTCTCGGG GACAAAAGGC CAAGTCAATG GAAGGGTTCC AGGACCTGCT TAACATGCGT 
CCGGACCAGT CCAATGTGAG GCGGATGCAT ACAGCAGTGA AGCTCAATGA GGTTATAGTT 
AACAAGTCCC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGGCCACC CCGAAACCCT 
GAGGGTGACG AAAACTACAT GGAGTTCCTG GAAGTACTCA CTGAAGGACT AGAGCGAGTC 
CTTCTTGTCC GAGGTGGTGG CAGTGAAGTG ATCACCATTT ATTCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019824019.1
CDS	1217..4624
Translation	MHPPETTTKMASVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSEMSGATTSLATVALDQASDRTSNPQDVTEDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLSRLANYTNLTQGAKEHEEAENITEGKKKPTKTPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGVLQAFAIVLICCCCnullMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYIVPRAAIFQSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKSSFAPPHFPVCMLGNRTLSSRHIDVCSKTKEVNNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVTSIQGIPGLASGVITENLWSNYLPKGEIIEKPSAKSSEVLGSLNHEYVLVDITTSFTLLVGIFFPSVTGIMAGSNRSGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPILSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYKYIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKAGKGLTIVGSVIVGNFLENYGEALAAEQTIKHLMEAEKVKGFSQLVVAAKLREGISHLIQSCGLGGMKHNTVVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNISFFPSNVEQFSEGNIDVWWIVHDGGMLMLLPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMMEQRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDDETETYQEKVHMTWTKDKYMASRGQKAKSMEGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENYMEFLEVLTEGLERVLLVRGGGSEVITIYS

Target ORF information:

RefSeq Version	XM_019968460.1
Organism	Bos indicus(Bos taurus indicus)
Definition	Bos indicus solute carrier family 12 member 6 (SLC12A6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019968460.1

ORF Insert Sequence:

ATGCATCCTC CAGAAACCAC CACCAAGATG GCTTCAGTTC GGTTCATGGT GACACCAACA 
AAGATCGATG ACATTCCAGG TTTGTCCGAC ACCAGTCCGG ACCTCAGCTC TCGATCCAGT 
TCCCGAGTAA GATTTAGCTC CCGGGAAAGT GTGCCGGAAA CCAGCCGTAG TGAGCCTATG 
AGTGAGATGT CTGGAGCCAC CACTTCCCTG GCAACGGTTG CACTGGATCA AGCCAGTGAC 
CGGACATCTA ACCCCCAGGA TGTTACTGAG GATGATGGAC ATAAGAAAGC GCGAAATGCT 
TATCTTAACA ATTCCAATTA TGAAGAAGGA GATGAATATT TTGATAAGAA TTTGGCACTC 
TTTGAGGAAG AAATGGACAC CAGACCAAAG GTGTCTTCTC TCCTTAGCCG CTTGGCCAAT 
TATACTAATC TGACACAAGG AGCAAAGGAA CATGAAGAGG CAGAGAACAT CACTGAAGGG 
AAAAAGAAGC CCACCAAGAC TCCCCAAATG GGTACCTTCA TGGGTGTCTA CCTCCCATGT 
CTACAAAATA TTTTTGGAGT GATCCTGTTT CTGCGCCTCA CATGGGTGGT GGGCACAGCT 
GGAGTTCTGC AGGCCTTTGC AATTGTCCTT ATCTGCTGCT GCTGTACRAT GTTGACTGCT 
ATCTCCATGA GTGCCATTGC CACTAATGGA GTGGTGCCAG CTGGAGGCTC ATACTTCATG 
ATTTCCCGAG CACTGGGCCC GGAGTTTGGT GGAGCTGTTG GCCTCTGCTT CTATCTTGGT 
ACCACATTTG CAGCAGCTAT GTATATCCTT GGTGCCATTG AAATATTTCT GGTATATATC 
GTCCCTCGAG CTGCCATCTT TCAGAGTGAC GATGCACTCA AGGAGTCAGC AGCCATGCTA 
AATAACATGC GTGTCTATGG GACAGCCTTC TTGGTCCTCA TGGTATTGGT GGTATTCATC 
GGCGTACGCT ATGTGAACAA GTTTGCCTCA CTCTTTCTGG CCTGTGTCAT TGTGTCCATC 
TTGGCCATCT ACGCTGGAGC CATCAAGTCT TCTTTCGCAC CTCCACACTT CCCGGTCTGC 
ATGCTGGGCA ACCGCACCCT TTCATCAAGA CATATTGACG TTTGCTCAAA GACCAAGGAA 
GTTAACAACA TGACAGTGCC GTCAAAGCTG TGGGGCTTCT TCTGTAACTC AAGTCAGTTT 
TTCAACGCCA CTTGCGATGA GTACTTTGTT CACAATAATG TCACTTCGAT CCAGGGCATC 
CCTGGATTGG CTAGCGGTGT CATTACAGAG AATCTTTGGA GTAATTATCT ACCAAAGGGA 
GAGATCATTG AGAAGCCCTC AGCCAAATCT TCCGAAGTCT TAGGCAGCTT AAACCATGAG 
TATGTCCTTG TGGACATCAC CACCTCTTTC ACTCTTCTGG TGGGCATCTT CTTTCCCTCT 
GTCACAGGCA TCATGGCAGG ATCAAACAGA TCTGGGGATC TGAAAGATGC TCAAAAGTCC 
ATTCCCATTG GCACTATCCT CGCCATCCTG ACCACCTCCT TTGTCTATTT AAGCAACGTT 
GTCCTTTTTG GTGCATGTAT TGAAGGTGTT GTCCTCAGAG ACAAGTTTGG AGATGCTGTG 
AAAGGTAATC TGGTGGTAGG AACCTTATCT TGGCCATCCC CGTGGGTGAT TGTTATTGGC 
TCCTTCTTCT CCACATGTGG GGCTGGACTT CAGAGTCTCA CAGGAGCACC GAGGCTGCTA 
CAGGCTATAG CCAAGGATAA CATTATACCC TTTCTGAGGG TTTTTGGTCA CAGCAAAGCC 
AATGGGGAAC CTACCTGGGC TTTACTTCTG ACTGCTGCCA TTGCAGAACT GGGAATCCTT 
ATTGCCTCCC TGGATCTCGT GGCTCCAATT CTTTCCATGT TTTTTCTCAT GTGTTACCTC 
TTTGTGAATT TGGCATGTGC CTTGCAAACA TTGCTTCGAA CACCCAACTG GAGACCCCGG 
TTCCGCTACT ACCACTGGGC ACTCTCTTTC ATGGGAATGA GTATCTGTCT GGCTCTGATG 
TTCATTTCTT CCTGGTATTA TGCCATCGTA GCTATGGTAA TAGCTGGTAT GATCTACAAA 
TACATCGAGT ACCAAGGAGC TGAGAAAGAA TGGGGTGATG GTATCCGCGG GCTGTCCCTC 
AGTGCAGCCC GATTTGCTTT GCTTAGGCTG GAGGAAGGAC CACCACACAC TAAAAACTGG 
AGGCCTCAGT TACTTGTATT GCTGAAACTA GATGAAGACC TACACGTCAA GCATCCTCGT 
CTCCTCACCT TTGCTTCACA GCTTAAGGCA GGAAAGGGTC TCACAATTGT GGGCTCTGTC 
ATCGTCGGGA ACTTCCTGGA GAACTATGGT GAAGCGTTAG CTGCTGAGCA GACCATAAAG 
CATTTAATGG AGGCAGAGAA GGTGAAAGGG TTCTCACAGC TGGTGGTGGC CGCCAAACTG 
AGGGAGGGCA TTTCCCACCT CATCCAGTCG TGTGGCCTTG GGGGCATGAA GCACAACACA 
GTGGTGATGG GATGGCCAAA TGGCTGGCGC CAGAGTGAAG ATGCTCGAGC TTGGAAGACT 
TTTATTGGCA CAGTTCGAGT GACAACTGCT GCCCATCTGG CCCTGCTGGT GGCCAAAAAC 
ATCTCCTTCT TTCCGAGCAA TGTGGAGCAG TTTTCTGAGG GCAACATTGA TGTGTGGTGG 
ATTGTACATG ATGGGGGGAT GCTCATGCTA TTACCATTCC TACTGAAACA GCATAAGGTG 
TGGCGAAAAT GCAGCATACG GATCTTCACA GTAGCCCAGC TAGAAGACAA CAGTATTCAG 
ATGAAGAAGG ACCTGGCCAC CTTCTTGTAT CACCTTCGCA TTGAGGCAGA GGTGGAAGTG 
GTAGAGATGC ATGACAGTGA CATATCCGCA TACACTTACG AGCGCACCCT GATGATGGAG 
CAGAGGTCAC AGATGCTGCG GCACATGCGC CTGTCCAAGA CGGAGCGAGA CCGAGAGGCA 
CAGCTGGTGA AAGATCGGAA CTCAATGCTA CGACTGACCA GCATCGGCTC TGACGAGGAT 
GACGAGACAG AGACTTATCA GGAGAAGGTG CACATGACGT GGACGAAAGA CAAGTATATG 
GCGTCTCGGG GACAAAAGGC CAAGTCAATG GAAGGGTTCC AGGACCTGCT TAACATGCGT 
CCGGACCAGT CCAATGTGAG GCGGATGCAT ACAGCAGTGA AGCTCAATGA GGTTATAGTT 
AACAAGTCCC ATGAAGCAAA GCTGGTTTTG TTGAATATGC CAGGGCCACC CCGAAACCCT 
GAGGGTGACG AAAACTACAT GGAGTTCCTG GAAGTACTCA CTGAAGGACT AGAGCGAGTC 
CTTCTTGTCC GAGGTGGTGG CAGTGAAGTG ATCACCATTT ATTCATAA