LOC100747574 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100747574
Entrez Gene ID	100747574
Full Name	activated CDC42 kinase 1
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012389176.1	XP_012244599.1	activated CDC42 kinase 1
XM_003491811.3	XP_003491859.1	activated CDC42 kinase 1
XM_012389178.2	XP_012244601.1	activated CDC42 kinase 1
XM_012389177.2	XP_012244600.1	activated CDC42 kinase 1
XM_003491811.2	XP_003491859.1	activated CDC42 kinase 1
XM_012389177.1	XP_012244600.1	activated CDC42 kinase 1
XM_024369704.1	XP_024225472.1	activated CDC42 kinase 1
XM_012389178.1	XP_012244601.1	activated CDC42 kinase 1

Pathways from BioSystems
Gene Ontology

The following LOC100747574 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100747574 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBo107395	XM_012389178.2 Latest version!	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo107395	XM_024369704.1 Latest version!	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo107395	XM_012389176.1 Latest version!	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo107395	XM_012389177.2 Latest version!	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo107395	XM_003491811.3 Latest version!	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
View Old Accession Versions >>
OBo107395	XM_003491811.2	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo107395	XM_012389177.1	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBo107395	XM_012389178.1	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100747574 ( XM_012389176.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012244599.1 Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo107395
Clone ID Related Accession (Same CDS sequence)	XM_012389176.1 , XM_003491811.3 , XM_012389178.2 , XM_012389177.2 , XM_003491811.2 , XM_012389177.1 , XM_024369704.1 , XM_012389178.1
Accession Version	XM_012389176.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	activated CDC42 kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012244599.1
CDS	556..4311
Translation	MADDEGTEWLQELLHDVQLSQFFTRIRDDLQVTRLHHFDHVQPEDLEQIGLGKPGIRRLLEAVKKKRTTQWKKSLMTKIRPGSSTKSNKRSSQPVEGSSVLTCLIQDKDVTLSDKLGDGSFGVVRRGQWTSPSGRILPVAVKVLKADALTQPNVIEDFVAEVQAMHTLDHHNLIRLYGVVLSQPMMMVTELAPLGALLDYLRKQCSQISVLTLCNYALQVVTGMAYLEAKRFLHRDLACRNVLLSSVDKVKIGDFGLMRALPQQEDCYVMTEHKKVPFPWCAPESLKARKFSHASDVWMFGVTLWEMLTFGEEPWIGLKGSEILRKIDKDGERLHEPEATPPDMYQLMLRCWAREPSERPTFASLKSSLTGMVPTVMKAISHFTMAGKMTIEQGDQIVILDGRPENYWWKGQNQRSFQIGLFPRCFVDPMRRKQREDISKPLENSFIHTGHGAPFGKSWGSPIYIDDVYLRNPMEPPDVVGVTVPPDNLVKDKYCGTPRSRKQFNYTKLQNDIGASPVKTLNASVPSGSQEGSLIDLSPEEMVNTSEAQSDTACRRVVNILDEPIDDLERQQQTSWQDEDPRTYANFPGNVDPAYSDPFDTSSVFMKLPHSRYYSHVPSDMSSRYFKTQTYGNVQNQDVGGSSQESTNYFTPESGSDVNHYSLNLSKESRNDSINLNVNVDTEENSAYSQNHYSEIDKPSPPMANWSNWPEDMQSEAQTYVNVSSRNLQTSDAPPPPPVAPNESPPKPKSNHDLAQSLNELTINSCNVSPKKLDPTFLAELEKHLGEKEASKNMITSQQNPDSSDPYASVNKLQDSTIPALRPPPQTIKRKSPQMDYRSTNLPSKVQNSWPSKSTNIQQPPRSQPEQRLETATEQMVGQIWQQSQAQQHSAYGTAQGNLNLLQIAPSNVSKPTFNHAQNSLPTANQASHFPNPTLPTGSHGITQIQNDLHQAHSSNVSKPPNVVFSEQVYAELKQTVPNLDQLSQNEFNTLYNKTVQQNILRNYYAASTSGAGDSASNLSHSHYLEGAAASSQARSGQSTRNIFGPGHPCDFSPHLKQPPVYNPPPPATWSPMKSVQNDLGQGQTAMKSNLASNLANSPNLLQLTHTNQPQEGAMTLQASSTRCLPPQMTETVVNTQLAPSTSAYPSGVSPPLTAASQQLVMSLNDEFRANKVMKVQREAADASQQEILTALQATGWDTNQAAKQILKDRQAKIESLFRLGLADRQQCEGALKQTEYDVDLAASLLLDQVK

Target ORF information:

RefSeq Version	XM_012389176.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012389176.1

ORF Insert Sequence:

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100747574 ( XM_003491811.3 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_003491859.1 Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo107395
Clone ID Related Accession (Same CDS sequence)	XM_012389176.1 , XM_003491811.3 , XM_012389178.2 , XM_012389177.2 , XM_003491811.2 , XM_012389177.1 , XM_024369704.1 , XM_012389178.1
Accession Version	XM_003491811.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	activated CDC42 kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_003491811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003491859.1
CDS	783..4538
Translation	MADDEGTEWLQELLHDVQLSQFFTRIRDDLQVTRLHHFDHVQPEDLEQIGLGKPGIRRLLEAVKKKRTTQWKKSLMTKIRPGSSTKSNKRSSQPVEGSSVLTCLIQDKDVTLSDKLGDGSFGVVRRGQWTSPSGRILPVAVKVLKADALTQPNVIEDFVAEVQAMHTLDHHNLIRLYGVVLSQPMMMVTELAPLGALLDYLRKQCSQISVLTLCNYALQVVTGMAYLEAKRFLHRDLACRNVLLSSVDKVKIGDFGLMRALPQQEDCYVMTEHKKVPFPWCAPESLKARKFSHASDVWMFGVTLWEMLTFGEEPWIGLKGSEILRKIDKDGERLHEPEATPPDMYQLMLRCWAREPSERPTFASLKSSLTGMVPTVMKAISHFTMAGKMTIEQGDQIVILDGRPENYWWKGQNQRSFQIGLFPRCFVDPMRRKQREDISKPLENSFIHTGHGAPFGKSWGSPIYIDDVYLRNPMEPPDVVGVTVPPDNLVKDKYCGTPRSRKQFNYTKLQNDIGASPVKTLNASVPSGSQEGSLIDLSPEEMVNTSEAQSDTACRRVVNILDEPIDDLERQQQTSWQDEDPRTYANFPGNVDPAYSDPFDTSSVFMKLPHSRYYSHVPSDMSSRYFKTQTYGNVQNQDVGGSSQESTNYFTPESGSDVNHYSLNLSKESRNDSINLNVNVDTEENSAYSQNHYSEIDKPSPPMANWSNWPEDMQSEAQTYVNVSSRNLQTSDAPPPPPVAPNESPPKPKSNHDLAQSLNELTINSCNVSPKKLDPTFLAELEKHLGEKEASKNMITSQQNPDSSDPYASVNKLQDSTIPALRPPPQTIKRKSPQMDYRSTNLPSKVQNSWPSKSTNIQQPPRSQPEQRLETATEQMVGQIWQQSQAQQHSAYGTAQGNLNLLQIAPSNVSKPTFNHAQNSLPTANQASHFPNPTLPTGSHGITQIQNDLHQAHSSNVSKPPNVVFSEQVYAELKQTVPNLDQLSQNEFNTLYNKTVQQNILRNYYAASTSGAGDSASNLSHSHYLEGAAASSQARSGQSTRNIFGPGHPCDFSPHLKQPPVYNPPPPATWSPMKSVQNDLGQGQTAMKSNLASNLANSPNLLQLTHTNQPQEGAMTLQASSTRCLPPQMTETVVNTQLAPSTSAYPSGVSPPLTAASQQLVMSLNDEFRANKVMKVQREAADASQQEILTALQATGWDTNQAAKQILKDRQAKIESLFRLGLADRQQCEGALKQTEYDVDLAASLLLDQVK

Target ORF information:

RefSeq Version	XM_003491811.3
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003491811.3

ORF Insert Sequence:

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100747574 ( XM_012389178.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012244601.1 Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X3, mRNA.

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CloneID	OBo107395
Clone ID Related Accession (Same CDS sequence)	XM_012389176.1 , XM_003491811.3 , XM_012389178.2 , XM_012389177.2 , XM_003491811.2 , XM_012389177.1 , XM_024369704.1 , XM_012389178.1
Accession Version	XM_012389178.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	activated CDC42 kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012389178.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012244601.1
CDS	304..4059
Translation	MADDEGTEWLQELLHDVQLSQFFTRIRDDLQVTRLHHFDHVQPEDLEQIGLGKPGIRRLLEAVKKKRTTQWKKSLMTKIRPGSSTKSNKRSSQPVEGSSVLTCLIQDKDVTLSDKLGDGSFGVVRRGQWTSPSGRILPVAVKVLKADALTQPNVIEDFVAEVQAMHTLDHHNLIRLYGVVLSQPMMMVTELAPLGALLDYLRKQCSQISVLTLCNYALQVVTGMAYLEAKRFLHRDLACRNVLLSSVDKVKIGDFGLMRALPQQEDCYVMTEHKKVPFPWCAPESLKARKFSHASDVWMFGVTLWEMLTFGEEPWIGLKGSEILRKIDKDGERLHEPEATPPDMYQLMLRCWAREPSERPTFASLKSSLTGMVPTVMKAISHFTMAGKMTIEQGDQIVILDGRPENYWWKGQNQRSFQIGLFPRCFVDPMRRKQREDISKPLENSFIHTGHGAPFGKSWGSPIYIDDVYLRNPMEPPDVVGVTVPPDNLVKDKYCGTPRSRKQFNYTKLQNDIGASPVKTLNASVPSGSQEGSLIDLSPEEMVNTSEAQSDTACRRVVNILDEPIDDLERQQQTSWQDEDPRTYANFPGNVDPAYSDPFDTSSVFMKLPHSRYYSHVPSDMSSRYFKTQTYGNVQNQDVGGSSQESTNYFTPESGSDVNHYSLNLSKESRNDSINLNVNVDTEENSAYSQNHYSEIDKPSPPMANWSNWPEDMQSEAQTYVNVSSRNLQTSDAPPPPPVAPNESPPKPKSNHDLAQSLNELTINSCNVSPKKLDPTFLAELEKHLGEKEASKNMITSQQNPDSSDPYASVNKLQDSTIPALRPPPQTIKRKSPQMDYRSTNLPSKVQNSWPSKSTNIQQPPRSQPEQRLETATEQMVGQIWQQSQAQQHSAYGTAQGNLNLLQIAPSNVSKPTFNHAQNSLPTANQASHFPNPTLPTGSHGITQIQNDLHQAHSSNVSKPPNVVFSEQVYAELKQTVPNLDQLSQNEFNTLYNKTVQQNILRNYYAASTSGAGDSASNLSHSHYLEGAAASSQARSGQSTRNIFGPGHPCDFSPHLKQPPVYNPPPPATWSPMKSVQNDLGQGQTAMKSNLASNLANSPNLLQLTHTNQPQEGAMTLQASSTRCLPPQMTETVVNTQLAPSTSAYPSGVSPPLTAASQQLVMSLNDEFRANKVMKVQREAADASQQEILTALQATGWDTNQAAKQILKDRQAKIESLFRLGLADRQQCEGALKQTEYDVDLAASLLLDQVK

Target ORF information:

RefSeq Version	XM_012389178.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012389178.2

ORF Insert Sequence:

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100747574 ( XM_012389177.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012244600.1 Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBo107395
Clone ID Related Accession (Same CDS sequence)	XM_012389176.1 , XM_003491811.3 , XM_012389178.2 , XM_012389177.2 , XM_003491811.2 , XM_012389177.1 , XM_024369704.1 , XM_012389178.1
Accession Version	XM_012389177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	activated CDC42 kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012389177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012244600.1
CDS	728..4483
Translation	MADDEGTEWLQELLHDVQLSQFFTRIRDDLQVTRLHHFDHVQPEDLEQIGLGKPGIRRLLEAVKKKRTTQWKKSLMTKIRPGSSTKSNKRSSQPVEGSSVLTCLIQDKDVTLSDKLGDGSFGVVRRGQWTSPSGRILPVAVKVLKADALTQPNVIEDFVAEVQAMHTLDHHNLIRLYGVVLSQPMMMVTELAPLGALLDYLRKQCSQISVLTLCNYALQVVTGMAYLEAKRFLHRDLACRNVLLSSVDKVKIGDFGLMRALPQQEDCYVMTEHKKVPFPWCAPESLKARKFSHASDVWMFGVTLWEMLTFGEEPWIGLKGSEILRKIDKDGERLHEPEATPPDMYQLMLRCWAREPSERPTFASLKSSLTGMVPTVMKAISHFTMAGKMTIEQGDQIVILDGRPENYWWKGQNQRSFQIGLFPRCFVDPMRRKQREDISKPLENSFIHTGHGAPFGKSWGSPIYIDDVYLRNPMEPPDVVGVTVPPDNLVKDKYCGTPRSRKQFNYTKLQNDIGASPVKTLNASVPSGSQEGSLIDLSPEEMVNTSEAQSDTACRRVVNILDEPIDDLERQQQTSWQDEDPRTYANFPGNVDPAYSDPFDTSSVFMKLPHSRYYSHVPSDMSSRYFKTQTYGNVQNQDVGGSSQESTNYFTPESGSDVNHYSLNLSKESRNDSINLNVNVDTEENSAYSQNHYSEIDKPSPPMANWSNWPEDMQSEAQTYVNVSSRNLQTSDAPPPPPVAPNESPPKPKSNHDLAQSLNELTINSCNVSPKKLDPTFLAELEKHLGEKEASKNMITSQQNPDSSDPYASVNKLQDSTIPALRPPPQTIKRKSPQMDYRSTNLPSKVQNSWPSKSTNIQQPPRSQPEQRLETATEQMVGQIWQQSQAQQHSAYGTAQGNLNLLQIAPSNVSKPTFNHAQNSLPTANQASHFPNPTLPTGSHGITQIQNDLHQAHSSNVSKPPNVVFSEQVYAELKQTVPNLDQLSQNEFNTLYNKTVQQNILRNYYAASTSGAGDSASNLSHSHYLEGAAASSQARSGQSTRNIFGPGHPCDFSPHLKQPPVYNPPPPATWSPMKSVQNDLGQGQTAMKSNLASNLANSPNLLQLTHTNQPQEGAMTLQASSTRCLPPQMTETVVNTQLAPSTSAYPSGVSPPLTAASQQLVMSLNDEFRANKVMKVQREAADASQQEILTALQATGWDTNQAAKQILKDRQAKIESLFRLGLADRQQCEGALKQTEYDVDLAASLLLDQVK

Target ORF information:

RefSeq Version	XM_012389177.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012389177.2

ORF Insert Sequence:

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100747574 ( XM_003491811.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_003491859.1 Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBo107395
Clone ID Related Accession (Same CDS sequence)	XM_012389176.1 , XM_003491811.3 , XM_012389178.2 , XM_012389177.2 , XM_003491811.2 , XM_012389177.1 , XM_024369704.1 , XM_012389178.1
Accession Version	XM_003491811.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	activated CDC42 kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Apr 28, 2015 this sequence version replaced XM_003491811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003491859.1
CDS	724..4479
Translation	MADDEGTEWLQELLHDVQLSQFFTRIRDDLQVTRLHHFDHVQPEDLEQIGLGKPGIRRLLEAVKKKRTTQWKKSLMTKIRPGSSTKSNKRSSQPVEGSSVLTCLIQDKDVTLSDKLGDGSFGVVRRGQWTSPSGRILPVAVKVLKADALTQPNVIEDFVAEVQAMHTLDHHNLIRLYGVVLSQPMMMVTELAPLGALLDYLRKQCSQISVLTLCNYALQVVTGMAYLEAKRFLHRDLACRNVLLSSVDKVKIGDFGLMRALPQQEDCYVMTEHKKVPFPWCAPESLKARKFSHASDVWMFGVTLWEMLTFGEEPWIGLKGSEILRKIDKDGERLHEPEATPPDMYQLMLRCWAREPSERPTFASLKSSLTGMVPTVMKAISHFTMAGKMTIEQGDQIVILDGRPENYWWKGQNQRSFQIGLFPRCFVDPMRRKQREDISKPLENSFIHTGHGAPFGKSWGSPIYIDDVYLRNPMEPPDVVGVTVPPDNLVKDKYCGTPRSRKQFNYTKLQNDIGASPVKTLNASVPSGSQEGSLIDLSPEEMVNTSEAQSDTACRRVVNILDEPIDDLERQQQTSWQDEDPRTYANFPGNVDPAYSDPFDTSSVFMKLPHSRYYSHVPSDMSSRYFKTQTYGNVQNQDVGGSSQESTNYFTPESGSDVNHYSLNLSKESRNDSINLNVNVDTEENSAYSQNHYSEIDKPSPPMANWSNWPEDMQSEAQTYVNVSSRNLQTSDAPPPPPVAPNESPPKPKSNHDLAQSLNELTINSCNVSPKKLDPTFLAELEKHLGEKEASKNMITSQQNPDSSDPYASVNKLQDSTIPALRPPPQTIKRKSPQMDYRSTNLPSKVQNSWPSKSTNIQQPPRSQPEQRLETATEQMVGQIWQQSQAQQHSAYGTAQGNLNLLQIAPSNVSKPTFNHAQNSLPTANQASHFPNPTLPTGSHGITQIQNDLHQAHSSNVSKPPNVVFSEQVYAELKQTVPNLDQLSQNEFNTLYNKTVQQNILRNYYAASTSGAGDSASNLSHSHYLEGAAASSQARSGQSTRNIFGPGHPCDFSPHLKQPPVYNPPPPATWSPMKSVQNDLGQGQTAMKSNLASNLANSPNLLQLTHTNQPQEGAMTLQASSTRCLPPQMTETVVNTQLAPSTSAYPSGVSPPLTAASQQLVMSLNDEFRANKVMKVQREAADASQQEILTALQATGWDTNQAAKQILKDRQAKIESLFRLGLADRQQCEGALKQTEYDVDLAASLLLDQVK

Target ORF information:

RefSeq Version	XM_003491811.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003491811.2

ORF Insert Sequence:

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100747574 ( XM_012389177.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012244600.1 Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X3, mRNA.

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CloneID	OBo107395
Clone ID Related Accession (Same CDS sequence)	XM_012389176.1 , XM_003491811.3 , XM_012389178.2 , XM_012389177.2 , XM_003491811.2 , XM_012389177.1 , XM_024369704.1 , XM_012389178.1
Accession Version	XM_012389177.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	activated CDC42 kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012244600.1
CDS	85..3840
Translation	MADDEGTEWLQELLHDVQLSQFFTRIRDDLQVTRLHHFDHVQPEDLEQIGLGKPGIRRLLEAVKKKRTTQWKKSLMTKIRPGSSTKSNKRSSQPVEGSSVLTCLIQDKDVTLSDKLGDGSFGVVRRGQWTSPSGRILPVAVKVLKADALTQPNVIEDFVAEVQAMHTLDHHNLIRLYGVVLSQPMMMVTELAPLGALLDYLRKQCSQISVLTLCNYALQVVTGMAYLEAKRFLHRDLACRNVLLSSVDKVKIGDFGLMRALPQQEDCYVMTEHKKVPFPWCAPESLKARKFSHASDVWMFGVTLWEMLTFGEEPWIGLKGSEILRKIDKDGERLHEPEATPPDMYQLMLRCWAREPSERPTFASLKSSLTGMVPTVMKAISHFTMAGKMTIEQGDQIVILDGRPENYWWKGQNQRSFQIGLFPRCFVDPMRRKQREDISKPLENSFIHTGHGAPFGKSWGSPIYIDDVYLRNPMEPPDVVGVTVPPDNLVKDKYCGTPRSRKQFNYTKLQNDIGASPVKTLNASVPSGSQEGSLIDLSPEEMVNTSEAQSDTACRRVVNILDEPIDDLERQQQTSWQDEDPRTYANFPGNVDPAYSDPFDTSSVFMKLPHSRYYSHVPSDMSSRYFKTQTYGNVQNQDVGGSSQESTNYFTPESGSDVNHYSLNLSKESRNDSINLNVNVDTEENSAYSQNHYSEIDKPSPPMANWSNWPEDMQSEAQTYVNVSSRNLQTSDAPPPPPVAPNESPPKPKSNHDLAQSLNELTINSCNVSPKKLDPTFLAELEKHLGEKEASKNMITSQQNPDSSDPYASVNKLQDSTIPALRPPPQTIKRKSPQMDYRSTNLPSKVQNSWPSKSTNIQQPPRSQPEQRLETATEQMVGQIWQQSQAQQHSAYGTAQGNLNLLQIAPSNVSKPTFNHAQNSLPTANQASHFPNPTLPTGSHGITQIQNDLHQAHSSNVSKPPNVVFSEQVYAELKQTVPNLDQLSQNEFNTLYNKTVQQNILRNYYAASTSGAGDSASNLSHSHYLEGAAASSQARSGQSTRNIFGPGHPCDFSPHLKQPPVYNPPPPATWSPMKSVQNDLGQGQTAMKSNLASNLANSPNLLQLTHTNQPQEGAMTLQASSTRCLPPQMTETVVNTQLAPSTSAYPSGVSPPLTAASQQLVMSLNDEFRANKVMKVQREAADASQQEILTALQATGWDTNQAAKQILKDRQAKIESLFRLGLADRQQCEGALKQTEYDVDLAASLLLDQVK

Target ORF information:

RefSeq Version	XM_012389177.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012389177.1

ORF Insert Sequence:

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100747574 ( XM_024369704.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_024225472.1 Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBo107395
Clone ID Related Accession (Same CDS sequence)	XM_012389176.1 , XM_003491811.3 , XM_012389178.2 , XM_012389177.2 , XM_003491811.2 , XM_012389177.1 , XM_024369704.1 , XM_012389178.1
Accession Version	XM_024369704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	activated CDC42 kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024225472.1
CDS	1429..5184
Translation	MADDEGTEWLQELLHDVQLSQFFTRIRDDLQVTRLHHFDHVQPEDLEQIGLGKPGIRRLLEAVKKKRTTQWKKSLMTKIRPGSSTKSNKRSSQPVEGSSVLTCLIQDKDVTLSDKLGDGSFGVVRRGQWTSPSGRILPVAVKVLKADALTQPNVIEDFVAEVQAMHTLDHHNLIRLYGVVLSQPMMMVTELAPLGALLDYLRKQCSQISVLTLCNYALQVVTGMAYLEAKRFLHRDLACRNVLLSSVDKVKIGDFGLMRALPQQEDCYVMTEHKKVPFPWCAPESLKARKFSHASDVWMFGVTLWEMLTFGEEPWIGLKGSEILRKIDKDGERLHEPEATPPDMYQLMLRCWAREPSERPTFASLKSSLTGMVPTVMKAISHFTMAGKMTIEQGDQIVILDGRPENYWWKGQNQRSFQIGLFPRCFVDPMRRKQREDISKPLENSFIHTGHGAPFGKSWGSPIYIDDVYLRNPMEPPDVVGVTVPPDNLVKDKYCGTPRSRKQFNYTKLQNDIGASPVKTLNASVPSGSQEGSLIDLSPEEMVNTSEAQSDTACRRVVNILDEPIDDLERQQQTSWQDEDPRTYANFPGNVDPAYSDPFDTSSVFMKLPHSRYYSHVPSDMSSRYFKTQTYGNVQNQDVGGSSQESTNYFTPESGSDVNHYSLNLSKESRNDSINLNVNVDTEENSAYSQNHYSEIDKPSPPMANWSNWPEDMQSEAQTYVNVSSRNLQTSDAPPPPPVAPNESPPKPKSNHDLAQSLNELTINSCNVSPKKLDPTFLAELEKHLGEKEASKNMITSQQNPDSSDPYASVNKLQDSTIPALRPPPQTIKRKSPQMDYRSTNLPSKVQNSWPSKSTNIQQPPRSQPEQRLETATEQMVGQIWQQSQAQQHSAYGTAQGNLNLLQIAPSNVSKPTFNHAQNSLPTANQASHFPNPTLPTGSHGITQIQNDLHQAHSSNVSKPPNVVFSEQVYAELKQTVPNLDQLSQNEFNTLYNKTVQQNILRNYYAASTSGAGDSASNLSHSHYLEGAAASSQARSGQSTRNIFGPGHPCDFSPHLKQPPVYNPPPPATWSPMKSVQNDLGQGQTAMKSNLASNLANSPNLLQLTHTNQPQEGAMTLQASSTRCLPPQMTETVVNTQLAPSTSAYPSGVSPPLTAASQQLVMSLNDEFRANKVMKVQREAADASQQEILTALQATGWDTNQAAKQILKDRQAKIESLFRLGLADRQQCEGALKQTEYDVDLAASLLLDQVK

Target ORF information:

RefSeq Version	XM_024369704.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024369704.1

ORF Insert Sequence:

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100747574 ( XM_012389178.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012244601.1 Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBo107395
Clone ID Related Accession (Same CDS sequence)	XM_012389176.1 , XM_003491811.3 , XM_012389178.2 , XM_012389177.2 , XM_003491811.2 , XM_012389177.1 , XM_024369704.1 , XM_012389178.1
Accession Version	XM_012389178.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	activated CDC42 kinase 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_177056.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012244601.1
CDS	220..3975
Translation	MADDEGTEWLQELLHDVQLSQFFTRIRDDLQVTRLHHFDHVQPEDLEQIGLGKPGIRRLLEAVKKKRTTQWKKSLMTKIRPGSSTKSNKRSSQPVEGSSVLTCLIQDKDVTLSDKLGDGSFGVVRRGQWTSPSGRILPVAVKVLKADALTQPNVIEDFVAEVQAMHTLDHHNLIRLYGVVLSQPMMMVTELAPLGALLDYLRKQCSQISVLTLCNYALQVVTGMAYLEAKRFLHRDLACRNVLLSSVDKVKIGDFGLMRALPQQEDCYVMTEHKKVPFPWCAPESLKARKFSHASDVWMFGVTLWEMLTFGEEPWIGLKGSEILRKIDKDGERLHEPEATPPDMYQLMLRCWAREPSERPTFASLKSSLTGMVPTVMKAISHFTMAGKMTIEQGDQIVILDGRPENYWWKGQNQRSFQIGLFPRCFVDPMRRKQREDISKPLENSFIHTGHGAPFGKSWGSPIYIDDVYLRNPMEPPDVVGVTVPPDNLVKDKYCGTPRSRKQFNYTKLQNDIGASPVKTLNASVPSGSQEGSLIDLSPEEMVNTSEAQSDTACRRVVNILDEPIDDLERQQQTSWQDEDPRTYANFPGNVDPAYSDPFDTSSVFMKLPHSRYYSHVPSDMSSRYFKTQTYGNVQNQDVGGSSQESTNYFTPESGSDVNHYSLNLSKESRNDSINLNVNVDTEENSAYSQNHYSEIDKPSPPMANWSNWPEDMQSEAQTYVNVSSRNLQTSDAPPPPPVAPNESPPKPKSNHDLAQSLNELTINSCNVSPKKLDPTFLAELEKHLGEKEASKNMITSQQNPDSSDPYASVNKLQDSTIPALRPPPQTIKRKSPQMDYRSTNLPSKVQNSWPSKSTNIQQPPRSQPEQRLETATEQMVGQIWQQSQAQQHSAYGTAQGNLNLLQIAPSNVSKPTFNHAQNSLPTANQASHFPNPTLPTGSHGITQIQNDLHQAHSSNVSKPPNVVFSEQVYAELKQTVPNLDQLSQNEFNTLYNKTVQQNILRNYYAASTSGAGDSASNLSHSHYLEGAAASSQARSGQSTRNIFGPGHPCDFSPHLKQPPVYNPPPPATWSPMKSVQNDLGQGQTAMKSNLASNLANSPNLLQLTHTNQPQEGAMTLQASSTRCLPPQMTETVVNTQLAPSTSAYPSGVSPPLTAASQQLVMSLNDEFRANKVMKVQREAADASQQEILTALQATGWDTNQAAKQILKDRQAKIESLFRLGLADRQQCEGALKQTEYDVDLAASLLLDQVK

Target ORF information:

RefSeq Version	XM_012389178.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens activated CDC42 kinase 1 (LOC100747574), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012389178.1

ORF Insert Sequence:

ATGGCAGATG ATGAGGGTAC AGAATGGCTG CAAGAACTGT TGCACGATGT TCAACTTTCA 
CAGTTTTTCA CCAGAATAAG AGATGACCTA CAAGTCACTC GACTACACCA CTTTGACCAT 
GTACAACCGG AAGATCTTGA ACAGATAGGA TTAGGAAAGC CAGGTATTAG AAGATTACTA 
GAAGCAGTGA AGAAAAAGAG AACTACGCAA TGGAAGAAAA GCTTGATGAC CAAGATCAGG 
CCTGGGAGTA GCACAAAAAG CAATAAAAGA TCTTCTCAAC CAGTTGAAGG TTCCTCAGTG 
CTAACATGTC TCATTCAAGA TAAAGATGTG ACACTGTCTG ATAAACTCGG TGATGGTAGT 
TTTGGTGTAG TGAGACGTGG ACAATGGACT TCTCCTTCTG GTAGGATATT ACCAGTTGCT 
GTAAAAGTTC TGAAGGCAGA TGCTCTGACT CAGCCAAATG TTATTGAAGA TTTTGTGGCA 
GAAGTTCAAG CAATGCACAC ATTGGATCAT CACAATCTCA TTAGGTTATA TGGAGTAGTG 
TTATCACAAC CGATGATGAT GGTGACAGAA CTAGCCCCTC TGGGAGCCCT GCTCGATTAT 
CTGCGAAAAC AGTGCAGTCA AATTTCAGTA TTAACGTTGT GTAACTACGC TTTGCAAGTG 
GTCACGGGTA TGGCATACTT AGAAGCGAAA CGTTTTCTGC ACCGCGACTT AGCTTGTAGG 
AACGTTCTTC TAAGTTCAGT GGACAAAGTG AAGATCGGTG ATTTCGGCCT AATGCGAGCA 
CTGCCGCAAC AAGAAGACTG TTATGTGATG ACTGAACATA AAAAAGTGCC GTTTCCTTGG 
TGTGCTCCAG AATCTTTAAA AGCGCGTAAA TTCAGTCACG CGTCAGATGT GTGGATGTTT 
GGTGTGACAT TGTGGGAAAT GTTAACGTTT GGTGAAGAGC CGTGGATAGG TTTAAAAGGA 
TCGGAGATTC TTCGAAAAAT AGACAAGGAT GGTGAACGAT TACACGAACC AGAAGCAACA 
CCACCAGATA TGTACCAATT AATGCTCCGT TGCTGGGCTC GGGAGCCTTC AGAACGACCA 
ACCTTCGCTT CTCTAAAATC TTCTCTCACG GGAATGGTCC CAACTGTGAT GAAAGCGATC 
AGTCACTTCA CGATGGCTGG CAAAATGACC ATAGAGCAGG GTGATCAGAT AGTCATTCTA 
GACGGACGGC CGGAGAATTA TTGGTGGAAA GGTCAGAACC AGCGAAGTTT CCAAATTGGG 
CTTTTTCCTC GTTGTTTCGT CGATCCTATG AGACGAAAAC AGCGTGAGGA CATCAGCAAG 
CCGTTGGAGA ATTCTTTTAT CCATACTGGT CATGGTGCAC CTTTTGGTAA AAGTTGGGGT 
AGTCCTATTT ACATCGACGA CGTGTATCTT CGAAATCCTA TGGAGCCACC TGACGTCGTT 
GGAGTAACTG TTCCTCCAGA TAATCTCGTG AAGGATAAAT ACTGCGGTAC ACCAAGATCG 
CGAAAACAGT TTAATTATAC AAAATTACAG AATGATATCG GAGCTAGTCC TGTGAAAACG 
TTAAACGCCT CTGTTCCTAG TGGCAGTCAG GAGGGCAGTT TGATAGATCT GTCCCCGGAG 
GAGATGGTGA ATACGAGTGA GGCGCAATCG GACACTGCCT GTCGACGAGT CGTCAATATC 
CTTGACGAGC CTATTGACGA TTTGGAACGG CAACAGCAGA CGAGTTGGCA GGACGAGGAT 
CCTCGAACTT ATGCCAACTT CCCTGGCAAC GTCGACCCGG CTTATTCAGA TCCGTTCGAT 
ACTTCCTCGG TATTCATGAA GCTGCCTCAT TCGAGATATT ACAGTCACGT TCCCTCCGAC 
ATGAGTAGCA GGTATTTCAA GACACAAACG TATGGAAACG TACAAAATCA GGACGTTGGC 
GGTAGCAGCC AGGAGAGTAC AAATTATTTC ACACCAGAGT CCGGCTCGGA TGTGAACCAT 
TATTCCCTTA ATCTGAGCAA GGAGAGTAGA AATGATAGTA TCAATTTGAA CGTGAACGTG 
GACACAGAGG AAAATAGTGC GTATAGTCAG AATCACTATA GTGAGATCGA TAAGCCTAGT 
CCACCTATGG CAAATTGGTC TAATTGGCCC GAAGATATGC AAAGTGAAGC GCAGACATAC 
GTGAATGTCT CTAGTCGGAA CTTGCAGACC TCCGACGCAC CTCCTCCTCC ACCTGTGGCT 
CCCAACGAGA GTCCTCCGAA ACCAAAATCG AATCACGACT TGGCCCAAAG TTTGAATGAG 
CTAACAATTA ATTCTTGCAA CGTTTCTCCT AAAAAGTTGG ACCCTACTTT CCTAGCTGAG 
CTGGAGAAGC ATTTAGGTGA AAAGGAGGCG AGCAAGAACA TGATCACTAG CCAACAGAAT 
CCAGATTCGT CTGATCCGTA TGCGTCTGTT AATAAATTAC AAGATAGTAC AATTCCAGCA 
TTAAGGCCTC CACCTCAGAC TATCAAACGT AAGTCTCCTC AGATGGACTA TAGAAGCACC 
AATCTACCTA GCAAGGTTCA GAATTCGTGG CCGTCGAAGA GTACGAACAT ACAGCAGCCT 
CCCAGGTCGC AGCCTGAACA ACGCTTAGAA ACGGCAACAG AACAGATGGT GGGTCAAATC 
TGGCAGCAGT CTCAAGCTCA GCAGCATAGT GCCTATGGTA CTGCTCAGGG TAACCTGAAT 
CTGTTGCAAA TAGCTCCTAG CAATGTGTCC AAACCAACCT TTAACCACGC ACAGAACTCG 
CTACCGACGG CAAACCAGGC TAGCCACTTT CCAAATCCCA CATTACCAAC TGGCAGTCAT 
GGGATAACGC AGATTCAAAA TGATCTTCAC CAAGCACACT CCAGTAACGT TTCAAAGCCT 
CCAAATGTGG TGTTTTCGGA ACAAGTGTAC GCGGAGTTGA AACAGACTGT ACCAAACCTA 
GACCAACTGT CTCAAAACGA GTTTAACACG CTATATAACA AGACAGTGCA GCAGAATATT 
CTAAGGAATT ACTATGCAGC TAGTACATCG GGTGCAGGTG ATTCAGCCAG CAATCTAAGT 
CATAGTCACT ACTTGGAGGG AGCTGCAGCC AGCTCCCAGG CTCGTTCTGG TCAGTCTACG 
AGGAATATTT TTGGTCCAGG TCATCCCTGT GACTTTAGTC CTCACCTGAA GCAGCCTCCG 
GTATACAATC CTCCGCCTCC GGCAACGTGG AGTCCCATGA AATCGGTTCA AAACGATCTT 
GGACAAGGCC AGACTGCAAT GAAGTCCAAT TTAGCTAGCA ATTTGGCTAA CAGTCCCAAT 
CTACTCCAGC TCACGCACAC TAACCAGCCT CAAGAGGGCG CCATGACGCT GCAAGCATCC 
TCGACGAGAT GTCTGCCTCC TCAGATGACA GAGACGGTCG TTAATACTCA ATTAGCCCCC 
TCCACGTCCG CGTACCCCTC TGGTGTCAGC CCCCCATTGA CCGCGGCCTC TCAACAGCTG 
GTCATGTCCC TCAACGATGA ATTTCGAGCG AACAAAGTAA TGAAGGTGCA GAGAGAGGCA 
GCTGACGCAT CACAACAGGA AATACTCACC GCGCTACAGG CAACTGGCTG GGACACTAAT 
CAAGCGGCCA AACAGATCCT GAAAGACAGG CAGGCGAAAA TCGAGTCCCT TTTTAGATTG 
GGCTTGGCAG ATCGACAACA GTGCGAGGGT GCTCTGAAGC AGACTGAATA CGATGTAGAT 
TTAGCGGCTT CCCTTTTGTT AGATCAGGTC AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6572

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オンライン注文

LOC100747574 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム