LOC100743211 cDNA ORF clone, Bombus impatiens(common eastern bumble bee)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100743211
Entrez Gene ID	100743211
Full Name	prosaposin
Gene Type	protein-coding
Organism	Bombus impatiens(common eastern bumble bee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012394050.2	XP_012249473.1	prosaposin
XM_012394050.1	XP_012249473.1	prosaposin

Pathways from BioSystems
Gene Ontology

The following LOC100743211 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100743211 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBo97653	XM_012394050.2 Latest version!	Bombus impatiens prosaposin (LOC100743211), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
OBo97653	XM_012394050.1	Bombus impatiens prosaposin (LOC100743211), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100743211 ( XM_012394050.2 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012249473.1 Bombus impatiens prosaposin (LOC100743211), mRNA.

Price & Availability↑

CloneID	OBo97653
Clone ID Related Accession (Same CDS sequence)	XM_012394050.2 , XM_012394050.1
Accession Version	XM_012394050.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-18
Organism	Bombus impatiens(common eastern bumble bee)
Product	prosaposin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176483.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Mar 19, 2018 this sequence version replaced XM_012394050.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bombus impatiens Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATTT TGATCGCGCT TTGCGCGATC CTTGCTGTAT CTACAGCAAG GGTAGTTATT 
AATGCTGAGG GACCAGCAAA TCTTTACCTT TTGGGTCAAC AGGAATGTAC ATGGGGACCT 
TCATATTGGT GTGAAAATAT AAAAACTGCG GCAGGCTGTA ATGCAACTGC ACATTGTATC 
AAAACGATAT GGAAGGATAT GAAAGTACCA CAGGATAATG ACAGTGTATG TAATGTTTGT 
ATGGACATGG TTCGGCAAGC TCGTGACCAA CTAGAGAGCA ATCAAACCCA AGAGGATTTA 
AAAACTGTTT TTGAAGGAAG CTGTAGGCTT ATTCGCCTAA ATGTAATTGT GAAAGAATGT 
ATAACAATTG TAGATCAATT TATCCCAGAA CTTGTTGAAA CATTAGCGTC TGAAATGAAT 
CCATCTATTG TTTGTTCTGT TGCGGGACTT TGTAATTCCG CTCGTATGGA CAAATTGTTA 
ATGGAATATG GACAACTTGA ATCTAAGACT AAGGAAGTAA AACCTCGATC TTTGGAAAAA 
GATGAATACG AACCTGATGA ATGTTCAAAA TGTTACACAG TAGCAGCTCA TATGGAACAC 
AAACTAAATA ATACCCCTCG CGATAAAGTG TTACAACAAA TGCTTAATGT ATGTGCAGAA 
CTTGGTACTT ACTCAGATGC TTGTTCAGCT ACGATCTTAA CTTACTTTGA CACAATATAT 
GCTCAATTGC AAGAAAGTTT CAATGCTCAA AATGTTTGTC ATTTATCTGG TCAGTGTAGT 
GGTAAATTCC ACAAACATGA AGATGCTGAT AAAGTTTTGA AAGTAGAAAT CAGACCACTT 
TCAAGTGTGG GTATGGTCGA TGTAAATGAC GACCTGCCAT GCAAATTGTG TGAACAACTA 
GTGGGACATT TAAGAGATCT TTTAGTAGCT AATACTACAG AAGCAGAATT TAAAGAAGTT 
CTGAAAGGAT TATGTAAACA AACCAAATCA TTTTCTTCTG AGTGTCTGGC TATAGTTGAT 
GAATATTATC CAGAAATATA TGAATATCTT ACAAAAGGTT TAAATTCCAA TATTGTTTGT 
CAAATGGGTG GAATATGCCC TACTCCAGAC AAAGCATTGC AGAATGAGCC AATTTGGCCT 
TTAGTACCTA AATCTGCGGC TGCAATAGGA ACACGTATTT TCCAAAATGC AAACAAGGCC 
ATGGAAGCTG ATAATAGTGA ACATCTGAGC AAATCAGAAG TTGAAGCAAT GCAATTGCCA 
ATTGAAAGGT TGGTACCGTT CCATCAATTA CAGGGTCTGT CAGACACTAA GAATATAGAA 
GATGATAGTA ACGAACAACT GAGTAAATCA GAAGTTGAAG CAATGCAATT GCCTATTGAA 
AGAACAGTAC CATTCCCTGT ATCAGAAAGT TTATTAGACA CTAAAGGGAC AGAAACATGT 
GCGTTATGCG AATATATATT ACATTTTATA CAAGAAGCTA TAACGGATCC TACCACAGAG 
GATAAAGTAA AGGAAGCATT AGGGAAAGTA TGTAAGAAAT TACCTGAATC TATAGAAAAT 
CAATGTACAC AGTTTGTCGA TCTTTATGGA GATGCTATTG TAGCTATTTT GGTTCAAGAA 
ATTGATCCAT CTCAAATATG TCCAATGTTA CACCTGTGTC CAGATGCAAA GTTGATGGCA 
CTGTGGCACA GTGTTCCAGC AAAATATATG CTTGAAGAAA AAAAGAGCAA ACCTAGTTGT 
CCACTATGTT TACTTGCTGT GTCACAAATT TATGAAGTTA TAAAAGATAA TAAGACCGAG 
GCAAACATAC AAGCTCAACT GGATAAGCTA TGTATTCATT TGCCACAGAG TTTGGTCGAA 
GAATGTACAG ATTTAGTTAA AGCTTATAGT AAAGAACTTA TAGAGTTACT GCTGGCTGAT 
TTAACACCTC AAGAAGTATG TGTTTATATT AAATTGTGCG ATGCTTCAAA GAATCCTGGT 
CCAAGAGATG ATTTTATTAC GGATAAAGAT GGTGAAATTT TAACAAATGA AATACCTAAT 
TCTCCAGCAA GTACTGAAGT ATCAGAAAGT ACACAAGGGC CATATTGTGT AATCTGTGAA 
TTCACTATGC ATTATATTGA CAAAGTACTT GGTAATGAAA AGGAAAGAAA TAAAGTTGAA 
AAGGCAGTAC TTGGAGTATG TAATCATCTT CCAAAGTCTG TTTCTCAAGA ATGTAACCAA 
TTTGTTAGTA AATACGCTTC TGCTGTAATT GATGTCCTCA CAAAAGATGT CAAACCAAAG 
CAAGTTTGCA CTATGCTTGG ACTTTGTTCA TTATACATAC AGGAAATCAA AGCTTCTGTA 
ACAGAATGCG CTTTGTGCAA ATCTATTATA TCAGTAATAG ATGAATTTTT GGATGTACAA 
GATGTTGATA ACAGCATTAC GAAAGTGTTG TCTAAAGTAT GTAAACATTT ACCAGCAGAC 
AAAGAACATA AGTGCACCAC GCTTATTAAT TCCTATGGGC AAAGCATAAT AAATATTGTC 
AAGAAACGCG AAAATATTTA CAAAATATGT AGTAAAATAG GTCTTTGTGC ACCCAATGAT 
TATTCAATGG TGTCTTTGGA AAATTCGCGA ATTAAACGAT CGTACGAAAA AAATCGTATA 
AAGCACTGTA CATGGGGGCC TGTCTACTGG TGCTCTACTA ATGAAACAGC TCGTGAATGT 
AAGGCTGTTG AACATTGCAA AGAAAACGTA TGGAAGGCCG ATTTCGCTCC TCCTAAACAA 
ATCACTTTGA GCGAGACAGA ACCTAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012249473.1
CDS	284..3076
Translation	MKILIALCAILAVSTARVVINAEGPANLYLLGQQECTWGPSYWCENIKTAAGCNATAHCIKTIWKDMKVPQDNDSVCNVCMDMVRQARDQLESNQTQEDLKTVFEGSCRLIRLNVIVKECITIVDQFIPELVETLASEMNPSIVCSVAGLCNSARMDKLLMEYGQLESKTKEVKPRSLEKDEYEPDECSKCYTVAAHMEHKLNNTPRDKVLQQMLNVCAELGTYSDACSATILTYFDTIYAQLQESFNAQNVCHLSGQCSGKFHKHEDADKVLKVEIRPLSSVGMVDVNDDLPCKLCEQLVGHLRDLLVANTTEAEFKEVLKGLCKQTKSFSSECLAIVDEYYPEIYEYLTKGLNSNIVCQMGGICPTPDKALQNEPIWPLVPKSAAAIGTRIFQNANKAMEADNSEHLSKSEVEAMQLPIERLVPFHQLQGLSDTKNIEDDSNEQLSKSEVEAMQLPIERTVPFPVSESLLDTKGTETCALCEYILHFIQEAITDPTTEDKVKEALGKVCKKLPESIENQCTQFVDLYGDAIVAILVQEIDPSQICPMLHLCPDAKLMALWHSVPAKYMLEEKKSKPSCPLCLLAVSQIYEVIKDNKTEANIQAQLDKLCIHLPQSLVEECTDLVKAYSKELIELLLADLTPQEVCVYIKLCDASKNPGPRDDFITDKDGEILTNEIPNSPASTEVSESTQGPYCVICEFTMHYIDKVLGNEKERNKVEKAVLGVCNHLPKSVSQECNQFVSKYASAVIDVLTKDVKPKQVCTMLGLCSLYIQEIKASVTECALCKSIISVIDEFLDVQDVDNSITKVLSKVCKHLPADKEHKCTTLINSYGQSIINIVKKRENIYKICSKIGLCAPNDYSMVSLENSRIKRSYEKNRIKHCTWGPVYWCSTNETARECKAVEHCKENVWKADFAPPKQITLSETEPNV

Target ORF information:

RefSeq Version	XM_012394050.2
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens prosaposin (LOC100743211), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012394050.2

ORF Insert Sequence:

ATGAAGATTT TGATCGCGCT TTGCGCGATC CTTGCTGTAT CTACAGCAAG GGTAGTTATT 
AATGCTGAGG GACCAGCAAA TCTTTACCTT TTGGGTCAAC AGGAATGTAC ATGGGGACCT 
TCATATTGGT GTGAAAATAT AAAAACTGCG GCAGGCTGTA ATGCAACTGC ACATTGTATC 
AAAACGATAT GGAAGGATAT GAAAGTACCA CAGGATAATG ACAGTGTATG TAATGTTTGT 
ATGGACATGG TTCGGCAAGC TCGTGACCAA CTAGAGAGCA ATCAAACCCA AGAGGATTTA 
AAAACTGTTT TTGAAGGAAG CTGTAGGCTT ATTCGCCTAA ATGTAATTGT GAAAGAATGT 
ATAACAATTG TAGATCAATT TATCCCAGAA CTTGTTGAAA CATTAGCGTC TGAAATGAAT 
CCATCTATTG TTTGTTCTGT TGCGGGACTT TGTAATTCCG CTCGTATGGA CAAATTGTTA 
ATGGAATATG GACAACTTGA ATCTAAGACT AAGGAAGTAA AACCTCGATC TTTGGAAAAA 
GATGAATACG AACCTGATGA ATGTTCAAAA TGTTACACAG TAGCAGCTCA TATGGAACAC 
AAACTAAATA ATACCCCTCG CGATAAAGTG TTACAACAAA TGCTTAATGT ATGTGCAGAA 
CTTGGTACTT ACTCAGATGC TTGTTCAGCT ACGATCTTAA CTTACTTTGA CACAATATAT 
GCTCAATTGC AAGAAAGTTT CAATGCTCAA AATGTTTGTC ATTTATCTGG TCAGTGTAGT 
GGTAAATTCC ACAAACATGA AGATGCTGAT AAAGTTTTGA AAGTAGAAAT CAGACCACTT 
TCAAGTGTGG GTATGGTCGA TGTAAATGAC GACCTGCCAT GCAAATTGTG TGAACAACTA 
GTGGGACATT TAAGAGATCT TTTAGTAGCT AATACTACAG AAGCAGAATT TAAAGAAGTT 
CTGAAAGGAT TATGTAAACA AACCAAATCA TTTTCTTCTG AGTGTCTGGC TATAGTTGAT 
GAATATTATC CAGAAATATA TGAATATCTT ACAAAAGGTT TAAATTCCAA TATTGTTTGT 
CAAATGGGTG GAATATGCCC TACTCCAGAC AAAGCATTGC AGAATGAGCC AATTTGGCCT 
TTAGTACCTA AATCTGCGGC TGCAATAGGA ACACGTATTT TCCAAAATGC AAACAAGGCC 
ATGGAAGCTG ATAATAGTGA ACATCTGAGC AAATCAGAAG TTGAAGCAAT GCAATTGCCA 
ATTGAAAGGT TGGTACCGTT CCATCAATTA CAGGGTCTGT CAGACACTAA GAATATAGAA 
GATGATAGTA ACGAACAACT GAGTAAATCA GAAGTTGAAG CAATGCAATT GCCTATTGAA 
AGAACAGTAC CATTCCCTGT ATCAGAAAGT TTATTAGACA CTAAAGGGAC AGAAACATGT 
GCGTTATGCG AATATATATT ACATTTTATA CAAGAAGCTA TAACGGATCC TACCACAGAG 
GATAAAGTAA AGGAAGCATT AGGGAAAGTA TGTAAGAAAT TACCTGAATC TATAGAAAAT 
CAATGTACAC AGTTTGTCGA TCTTTATGGA GATGCTATTG TAGCTATTTT GGTTCAAGAA 
ATTGATCCAT CTCAAATATG TCCAATGTTA CACCTGTGTC CAGATGCAAA GTTGATGGCA 
CTGTGGCACA GTGTTCCAGC AAAATATATG CTTGAAGAAA AAAAGAGCAA ACCTAGTTGT 
CCACTATGTT TACTTGCTGT GTCACAAATT TATGAAGTTA TAAAAGATAA TAAGACCGAG 
GCAAACATAC AAGCTCAACT GGATAAGCTA TGTATTCATT TGCCACAGAG TTTGGTCGAA 
GAATGTACAG ATTTAGTTAA AGCTTATAGT AAAGAACTTA TAGAGTTACT GCTGGCTGAT 
TTAACACCTC AAGAAGTATG TGTTTATATT AAATTGTGCG ATGCTTCAAA GAATCCTGGT 
CCAAGAGATG ATTTTATTAC GGATAAAGAT GGTGAAATTT TAACAAATGA AATACCTAAT 
TCTCCAGCAA GTACTGAAGT ATCAGAAAGT ACACAAGGGC CATATTGTGT AATCTGTGAA 
TTCACTATGC ATTATATTGA CAAAGTACTT GGTAATGAAA AGGAAAGAAA TAAAGTTGAA 
AAGGCAGTAC TTGGAGTATG TAATCATCTT CCAAAGTCTG TTTCTCAAGA ATGTAACCAA 
TTTGTTAGTA AATACGCTTC TGCTGTAATT GATGTCCTCA CAAAAGATGT CAAACCAAAG 
CAAGTTTGCA CTATGCTTGG ACTTTGTTCA TTATACATAC AGGAAATCAA AGCTTCTGTA 
ACAGAATGCG CTTTGTGCAA ATCTATTATA TCAGTAATAG ATGAATTTTT GGATGTACAA 
GATGTTGATA ACAGCATTAC GAAAGTGTTG TCTAAAGTAT GTAAACATTT ACCAGCAGAC 
AAAGAACATA AGTGCACCAC GCTTATTAAT TCCTATGGGC AAAGCATAAT AAATATTGTC 
AAGAAACGCG AAAATATTTA CAAAATATGT AGTAAAATAG GTCTTTGTGC ACCCAATGAT 
TATTCAATGG TGTCTTTGGA AAATTCGCGA ATTAAACGAT CGTACGAAAA AAATCGTATA 
AAGCACTGTA CATGGGGGCC TGTCTACTGG TGCTCTACTA ATGAAACAGC TCGTGAATGT 
AAGGCTGTTG AACATTGCAA AGAAAACGTA TGGAAGGCCG ATTTCGCTCC TCCTAAACAA 
ATCACTTTGA GCGAGACAGA ACCTAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100743211 ( XM_012394050.1 ) cDNA ORF clone, Bombus impatiens(common eastern bumble bee) -> XP_012249473.1 Bombus impatiens prosaposin (LOC100743211), mRNA.

Price & Availability↑

CloneID	OBo97653
Clone ID Related Accession (Same CDS sequence)	XM_012394050.2 , XM_012394050.1
Accession Version	XM_012394050.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-04-27
Organism	Bombus impatiens(common eastern bumble bee)
Product	prosaposin
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_176483.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bombus impatiens Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGATTT TGATCGCGCT TTGCGCGATC CTTGCTGTAT CTACAGCAAG GGTAGTTATT 
AATGCTGAGG GACCAGCAAA TCTTTACCTT TTGGGTCAAC AGGAATGTAC ATGGGGACCT 
TCATATTGGT GTGAAAATAT AAAAACTGCG GCAGGCTGTA ATGCAACTGC ACATTGTATC 
AAAACGATAT GGAAGGATAT GAAAGTACCA CAGGATAATG ACAGTGTATG TAATGTTTGT 
ATGGACATGG TTCGGCAAGC TCGTGACCAA CTAGAGAGCA ATCAAACCCA AGAGGATTTA 
AAAACTGTTT TTGAAGGAAG CTGTAGGCTT ATTCGCCTAA ATGTAATTGT GAAAGAATGT 
ATAACAATTG TAGATCAATT TATCCCAGAA CTTGTTGAAA CATTAGCGTC TGAAATGAAT 
CCATCTATTG TTTGTTCTGT TGCGGGACTT TGTAATTCCG CTCGTATGGA CAAATTGTTA 
ATGGAATATG GACAACTTGA ATCTAAGACT AAGGAAGTAA AACCTCGATC TTTGGAAAAA 
GATGAATACG AACCTGATGA ATGTTCAAAA TGTTACACAG TAGCAGCTCA TATGGAACAC 
AAACTAAATA ATACCCCTCG CGATAAAGTG TTACAACAAA TGCTTAATGT ATGTGCAGAA 
CTTGGTACTT ACTCAGATGC TTGTTCAGCT ACGATCTTAA CTTACTTTGA CACAATATAT 
GCTCAATTGC AAGAAAGTTT CAATGCTCAA AATGTTTGTC ATTTATCTGG TCAGTGTAGT 
GGTAAATTCC ACAAACATGA AGATGCTGAT AAAGTTTTGA AAGTAGAAAT CAGACCACTT 
TCAAGTGTGG GTATGGTCGA TGTAAATGAC GACCTGCCAT GCAAATTGTG TGAACAACTA 
GTGGGACATT TAAGAGATCT TTTAGTAGCT AATACTACAG AAGCAGAATT TAAAGAAGTT 
CTGAAAGGAT TATGTAAACA AACCAAATCA TTTTCTTCTG AGTGTCTGGC TATAGTTGAT 
GAATATTATC CAGAAATATA TGAATATCTT ACAAAAGGTT TAAATTCCAA TATTGTTTGT 
CAAATGGGTG GAATATGCCC TACTCCAGAC AAAGCATTGC AGAATGAGCC AATTTGGCCT 
TTAGTACCTA AATCTGCGGC TGCAATAGGA ACACGTATTT TCCAAAATGC AAACAAGGCC 
ATGGAAGCTG ATAATAGTGA ACATCTGAGC AAATCAGAAG TTGAAGCAAT GCAATTGCCA 
ATTGAAAGGT TGGTACCGTT CCATCAATTA CAGGGTCTGT CAGACACTAA GAATATAGAA 
GATGATAGTA ACGAACAACT GAGTAAATCA GAAGTTGAAG CAATGCAATT GCCTATTGAA 
AGAACAGTAC CATTCCCTGT ATCAGAAAGT TTATTAGACA CTAAAGGGAC AGAAACATGT 
GCGTTATGCG AATATATATT ACATTTTATA CAAGAAGCTA TAACGGATCC TACCACAGAG 
GATAAAGTAA AGGAAGCATT AGGGAAAGTA TGTAAGAAAT TACCTGAATC TATAGAAAAT 
CAATGTACAC AGTTTGTCGA TCTTTATGGA GATGCTATTG TAGCTATTTT GGTTCAAGAA 
ATTGATCCAT CTCAAATATG TCCAATGTTA CACCTGTGTC CAGATGCAAA GTTGATGGCA 
CTGTGGCACA GTGTTCCAGC AAAATATATG CTTGAAGAAA AAAAGAGCAA ACCTAGTTGT 
CCACTATGTT TACTTGCTGT GTCACAAATT TATGAAGTTA TAAAAGATAA TAAGACCGAG 
GCAAACATAC AAGCTCAACT GGATAAGCTA TGTATTCATT TGCCACAGAG TTTGGTCGAA 
GAATGTACAG ATTTAGTTAA AGCTTATAGT AAAGAACTTA TAGAGTTACT GCTGGCTGAT 
TTAACACCTC AAGAAGTATG TGTTTATATT AAATTGTGCG ATGCTTCAAA GAATCCTGGT 
CCAAGAGATG ATTTTATTAC GGATAAAGAT GGTGAAATTT TAACAAATGA AATACCTAAT 
TCTCCAGCAA GTACTGAAGT ATCAGAAAGT ACACAAGGGC CATATTGTGT AATCTGTGAA 
TTCACTATGC ATTATATTGA CAAAGTACTT GGTAATGAAA AGGAAAGAAA TAAAGTTGAA 
AAGGCAGTAC TTGGAGTATG TAATCATCTT CCAAAGTCTG TTTCTCAAGA ATGTAACCAA 
TTTGTTAGTA AATACGCTTC TGCTGTAATT GATGTCCTCA CAAAAGATGT CAAACCAAAG 
CAAGTTTGCA CTATGCTTGG ACTTTGTTCA TTATACATAC AGGAAATCAA AGCTTCTGTA 
ACAGAATGCG CTTTGTGCAA ATCTATTATA TCAGTAATAG ATGAATTTTT GGATGTACAA 
GATGTTGATA ACAGCATTAC GAAAGTGTTG TCTAAAGTAT GTAAACATTT ACCAGCAGAC 
AAAGAACATA AGTGCACCAC GCTTATTAAT TCCTATGGGC AAAGCATAAT AAATATTGTC 
AAGAAACGCG AAAATATTTA CAAAATATGT AGTAAAATAG GTCTTTGTGC ACCCAATGAT 
TATTCAATGG TGTCTTTGGA AAATTCGCGA ATTAAACGAT CGTACGAAAA AAATCGTATA 
AAGCACTGTA CATGGGGGCC TGTCTACTGG TGCTCTACTA ATGAAACAGC TCGTGAATGT 
AAGGCTGTTG AACATTGCAA AGAAAACGTA TGGAAGGCCG ATTTCGCTCC TCCTAAACAA 
ATCACTTTGA GCGAGACAGA ACCTAATGTT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012249473.1
CDS	282..3074
Translation	MKILIALCAILAVSTARVVINAEGPANLYLLGQQECTWGPSYWCENIKTAAGCNATAHCIKTIWKDMKVPQDNDSVCNVCMDMVRQARDQLESNQTQEDLKTVFEGSCRLIRLNVIVKECITIVDQFIPELVETLASEMNPSIVCSVAGLCNSARMDKLLMEYGQLESKTKEVKPRSLEKDEYEPDECSKCYTVAAHMEHKLNNTPRDKVLQQMLNVCAELGTYSDACSATILTYFDTIYAQLQESFNAQNVCHLSGQCSGKFHKHEDADKVLKVEIRPLSSVGMVDVNDDLPCKLCEQLVGHLRDLLVANTTEAEFKEVLKGLCKQTKSFSSECLAIVDEYYPEIYEYLTKGLNSNIVCQMGGICPTPDKALQNEPIWPLVPKSAAAIGTRIFQNANKAMEADNSEHLSKSEVEAMQLPIERLVPFHQLQGLSDTKNIEDDSNEQLSKSEVEAMQLPIERTVPFPVSESLLDTKGTETCALCEYILHFIQEAITDPTTEDKVKEALGKVCKKLPESIENQCTQFVDLYGDAIVAILVQEIDPSQICPMLHLCPDAKLMALWHSVPAKYMLEEKKSKPSCPLCLLAVSQIYEVIKDNKTEANIQAQLDKLCIHLPQSLVEECTDLVKAYSKELIELLLADLTPQEVCVYIKLCDASKNPGPRDDFITDKDGEILTNEIPNSPASTEVSESTQGPYCVICEFTMHYIDKVLGNEKERNKVEKAVLGVCNHLPKSVSQECNQFVSKYASAVIDVLTKDVKPKQVCTMLGLCSLYIQEIKASVTECALCKSIISVIDEFLDVQDVDNSITKVLSKVCKHLPADKEHKCTTLINSYGQSIINIVKKRENIYKICSKIGLCAPNDYSMVSLENSRIKRSYEKNRIKHCTWGPVYWCSTNETARECKAVEHCKENVWKADFAPPKQITLSETEPNV

Target ORF information:

RefSeq Version	XM_012394050.1
Organism	Bombus impatiens(common eastern bumble bee)
Definition	Bombus impatiens prosaposin (LOC100743211), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012394050.1

ORF Insert Sequence:

ATGAAGATTT TGATCGCGCT TTGCGCGATC CTTGCTGTAT CTACAGCAAG GGTAGTTATT 
AATGCTGAGG GACCAGCAAA TCTTTACCTT TTGGGTCAAC AGGAATGTAC ATGGGGACCT 
TCATATTGGT GTGAAAATAT AAAAACTGCG GCAGGCTGTA ATGCAACTGC ACATTGTATC 
AAAACGATAT GGAAGGATAT GAAAGTACCA CAGGATAATG ACAGTGTATG TAATGTTTGT 
ATGGACATGG TTCGGCAAGC TCGTGACCAA CTAGAGAGCA ATCAAACCCA AGAGGATTTA 
AAAACTGTTT TTGAAGGAAG CTGTAGGCTT ATTCGCCTAA ATGTAATTGT GAAAGAATGT 
ATAACAATTG TAGATCAATT TATCCCAGAA CTTGTTGAAA CATTAGCGTC TGAAATGAAT 
CCATCTATTG TTTGTTCTGT TGCGGGACTT TGTAATTCCG CTCGTATGGA CAAATTGTTA 
ATGGAATATG GACAACTTGA ATCTAAGACT AAGGAAGTAA AACCTCGATC TTTGGAAAAA 
GATGAATACG AACCTGATGA ATGTTCAAAA TGTTACACAG TAGCAGCTCA TATGGAACAC 
AAACTAAATA ATACCCCTCG CGATAAAGTG TTACAACAAA TGCTTAATGT ATGTGCAGAA 
CTTGGTACTT ACTCAGATGC TTGTTCAGCT ACGATCTTAA CTTACTTTGA CACAATATAT 
GCTCAATTGC AAGAAAGTTT CAATGCTCAA AATGTTTGTC ATTTATCTGG TCAGTGTAGT 
GGTAAATTCC ACAAACATGA AGATGCTGAT AAAGTTTTGA AAGTAGAAAT CAGACCACTT 
TCAAGTGTGG GTATGGTCGA TGTAAATGAC GACCTGCCAT GCAAATTGTG TGAACAACTA 
GTGGGACATT TAAGAGATCT TTTAGTAGCT AATACTACAG AAGCAGAATT TAAAGAAGTT 
CTGAAAGGAT TATGTAAACA AACCAAATCA TTTTCTTCTG AGTGTCTGGC TATAGTTGAT 
GAATATTATC CAGAAATATA TGAATATCTT ACAAAAGGTT TAAATTCCAA TATTGTTTGT 
CAAATGGGTG GAATATGCCC TACTCCAGAC AAAGCATTGC AGAATGAGCC AATTTGGCCT 
TTAGTACCTA AATCTGCGGC TGCAATAGGA ACACGTATTT TCCAAAATGC AAACAAGGCC 
ATGGAAGCTG ATAATAGTGA ACATCTGAGC AAATCAGAAG TTGAAGCAAT GCAATTGCCA 
ATTGAAAGGT TGGTACCGTT CCATCAATTA CAGGGTCTGT CAGACACTAA GAATATAGAA 
GATGATAGTA ACGAACAACT GAGTAAATCA GAAGTTGAAG CAATGCAATT GCCTATTGAA 
AGAACAGTAC CATTCCCTGT ATCAGAAAGT TTATTAGACA CTAAAGGGAC AGAAACATGT 
GCGTTATGCG AATATATATT ACATTTTATA CAAGAAGCTA TAACGGATCC TACCACAGAG 
GATAAAGTAA AGGAAGCATT AGGGAAAGTA TGTAAGAAAT TACCTGAATC TATAGAAAAT 
CAATGTACAC AGTTTGTCGA TCTTTATGGA GATGCTATTG TAGCTATTTT GGTTCAAGAA 
ATTGATCCAT CTCAAATATG TCCAATGTTA CACCTGTGTC CAGATGCAAA GTTGATGGCA 
CTGTGGCACA GTGTTCCAGC AAAATATATG CTTGAAGAAA AAAAGAGCAA ACCTAGTTGT 
CCACTATGTT TACTTGCTGT GTCACAAATT TATGAAGTTA TAAAAGATAA TAAGACCGAG 
GCAAACATAC AAGCTCAACT GGATAAGCTA TGTATTCATT TGCCACAGAG TTTGGTCGAA 
GAATGTACAG ATTTAGTTAA AGCTTATAGT AAAGAACTTA TAGAGTTACT GCTGGCTGAT 
TTAACACCTC AAGAAGTATG TGTTTATATT AAATTGTGCG ATGCTTCAAA GAATCCTGGT 
CCAAGAGATG ATTTTATTAC GGATAAAGAT GGTGAAATTT TAACAAATGA AATACCTAAT 
TCTCCAGCAA GTACTGAAGT ATCAGAAAGT ACACAAGGGC CATATTGTGT AATCTGTGAA 
TTCACTATGC ATTATATTGA CAAAGTACTT GGTAATGAAA AGGAAAGAAA TAAAGTTGAA 
AAGGCAGTAC TTGGAGTATG TAATCATCTT CCAAAGTCTG TTTCTCAAGA ATGTAACCAA 
TTTGTTAGTA AATACGCTTC TGCTGTAATT GATGTCCTCA CAAAAGATGT CAAACCAAAG 
CAAGTTTGCA CTATGCTTGG ACTTTGTTCA TTATACATAC AGGAAATCAA AGCTTCTGTA 
ACAGAATGCG CTTTGTGCAA ATCTATTATA TCAGTAATAG ATGAATTTTT GGATGTACAA 
GATGTTGATA ACAGCATTAC GAAAGTGTTG TCTAAAGTAT GTAAACATTT ACCAGCAGAC 
AAAGAACATA AGTGCACCAC GCTTATTAAT TCCTATGGGC AAAGCATAAT AAATATTGTC 
AAGAAACGCG AAAATATTTA CAAAATATGT AGTAAAATAG GTCTTTGTGC ACCCAATGAT 
TATTCAATGG TGTCTTTGGA AAATTCGCGA ATTAAACGAT CGTACGAAAA AAATCGTATA 
AAGCACTGTA CATGGGGGCC TGTCTACTGG TGCTCTACTA ATGAAACAGC TCGTGAATGT 
AAGGCTGTTG AACATTGCAA AGAAAACGTA TGGAAGGCCG ATTTCGCTCC TCCTAAACAA 
ATCACTTTGA GCGAGACAGA ACCTAATGTT TAA