DDX58 cDNA ORF clone, Bison bison bison

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX58
Entrez Gene ID	104988320
Full Name	DExD/H-box helicase 58
Gene Type	protein-coding
Organism	Bison bison bison

mRNA and Protein(s)

mRNA	Protein	Name
XM_010839604.1	XP_010837906.1	probable ATP-dependent RNA helicase DDX58

Pathways from BioSystems
Gene Ontology

The following DDX58 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX58 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBi64497	XM_010839604.1 Latest version!	Bison bison bison DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX58 ( XM_010839604.1 ) cDNA ORF clone, Bison bison bison -> XP_010837906.1 Bison bison bison DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mRNA.

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CloneID	OBi64497
Clone ID Related Accession (Same CDS sequence)	XM_010839604.1
Accession Version	XM_010839604.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3054bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-12-30
Organism	Bison bison bison
Product	probable ATP-dependent RNA helicase DDX58
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011494695.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bison bison bison Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGG TTGATCAGGG AGCCTGGGGG TGCCCTGCCC CTAGTTTCAG TTTCGATTTT 
CCCTCCGCAG CCCGTCTTCC CCTGCAGCTC CGCCTCCGCG CTGGGCGCTC GCCTCGGCAA 
ATTTCTCGCG CTTCACGGCT GTGTTTAGGA AGAGCGGAGC CGAGCTCAGC GGAGCCCAGC 
AGAGCACAGG CGAAGCAGGG GGTCGCGGGG AGCGGAGCAG CAGGCATGAC GGCAGAGCAG 
CGGCGGAATT TGCACGCCTT CCGCGACTAT GTCAGGAAGA TCCTGGACCC TACCTACATC 
CTGAGCTACA TGACCCCCTG GTTTAGGGAC GATGTGGTGC AGCACATTCA GGCCAAGAAA 
AACAACAAGG GGCCCATGGA GGCTGCCTCC CTTTTCCTCC AGGTCCTACT GGAGCTCCAG 
GAGGAAGGCT GGTTCCGCGG CTTTTTGGAT GCCCTTCAAC AAGCAGGTTA TTCTGGACTT 
TATGAAGCCA TTGAAAGTTG GGATTTCCAA AAACTTGAAA AATTGGAGGA GTATAGATTA 
CTTTTAAAGC GTTTACAACC AGAATTTAAA ACCACAATCA ATCCAGAAGA TATCCTTCCT 
GAAATATCTG GATGTTTACT TAATCAAGAA TGTGAAGAAA TTATTCAGAT AAGTTCCAAC 
AAGGGGCTGA TGGCAGGTGC AGAGAAAATG GTTGAATGCC TTCTCAGATC AGACAAGGAG 
AACTGGCCCA AAACTTTGAA ACTTGCATTG GAGAAAGAAG AGAGCAAGTT CAGCGAACTG 
TGGATGGTAG AGAAAGGTGC AGAAAATGTT CAAATGAAAG ATCTTGAGGA TGATGAAATG 
AAAACTTTGG ATGTACACAT TGTCTACAAA GAAGAACCAG AAAGCCAGAA TCTTAGCCAG 
AATTCCTGTT CTTCAGAAGT GCCTCCTACT TATAGCCCAT TGAAACCAAG AAATTACCAA 
CTAGAGCTCA CTTTACCTGC TCAGAAGGGA AAGAATACAA TAATATGTGC TCCTACTGGT 
TGTGGAAAAA CCTTTGTTTC CCTTCTTATA TGCGAACATC ATCTTAAGAA ATTTCCACAA 
GGACGAAAGG GGAAAGTTGT CATTTTTGCT GTTCAAGTCC CACTGTATGA ACAGCAGAAA 
TCTGTGTTCT CAGAACATTT TGAAAGATTT GGGTACAAAG TTTCAGGCAT TTCTGGAGAG 
ACAGCTGACA ATATCTCTGT GGAACAGATT GTTGAGAACA ATGACATCAT CATTTTAACA 
CCACAGATTC TTGTGAACAG CCTTAAAGAT GGGACTATTC CATCACTCTC CATCTTTACT 
TTGATGATAT TTGATGAATG TCACAACACC AATAAACACC ATCCATATAA TATGATCATG 
TTTCATTATC TGGATCAGAA ACTGGGAGGA TCTTCAGACT CATTGCCCCA GGTCATTGGG 
CTGACTGCCT CAGTTGGCGT TGGAGATGCC AAAAACACAG CCGAAGCCAC AGAATATATC 
TGCAAACTGT GTGCTTCTCT CGACACAGCA GTGGTAACAA CAGTCAGAGA CAACTTGGAG 
GAACTGGAGG AGGTTGTTTA CAAGCCCCAG AAGTTTTTCA GGAAAGTGGA ATCACGGACT 
ACAGACAGAT TTAAACGCAT CATATCTCAG CTGATGGCAG AGACAGAGGC TCTAGCAAAG 
AGTATCTTTG AAGAACTTGG GACTGTAACT CTTGAAAACT TATCTCGAAT TCAAAATAGG 
AATTTTGGGA CACAGAAATA TGAACAGTGG ATTATTGCAG TTCAGAAAGC ATGCATGGTG 
TTTCAGATGC CAGACAAAGA GGAAGAAAGC AGGATTTGTA AAGCCCTGTT TTTGTACACG 
TCACATTTGC GGAAATACAA TGATGCCCTC ATTATCAATG AGGATGCACG GATGAAAGAT 
GCTCTGAATT ACTTGAAAAA CTTCTTCAAA AATGTCCGGG CAGCAGGATT TGATGCAATT 
GAGCAAGATC TCACTCAGAG ATTTGAAGAA AAGCTGTTGG AACTAGAAGG TATTTCCATG 
GATCCCAGCA ATGAGAACGC TAAACTCAAA GATCTCTGCT TCATCTTGCA AGAGGAATAC 
CACTTAAACC CAGAGACCAG AACCATCCTC TTTGTGAAAA CCCGAGCACT GGTGGATGCC 
TTAAAGAACT GGATTGAAGA AAATCCTAAA CTCAGCTTTC TAAAACCTGG CATATTGACT 
GGACGTGGCA GAACAAATCA GACAATGGGA ATGACGCTCC CAGCACAGAA GTGTGCACTG 
GATGCATTCC GAACCAACAG AGACAGCAAG ATTCTAATTG CTACCTCGGT CGCCGATGAA 
GGCATTGACA TTGCTCAGTG CAATCTGGTC ATCCTCTATG AGTATGTGGG CAATGTCATC 
AAAATGATCC AGACCAGAGG CAGAGGAAGA GCAAGAGGGA GCAAGTGCTT CCTTCTGACT 
AGTAATGCTG ATGTAATCGA AAAAGAAAAA CTGAACATCT GCCAAGAAAA AATGATGAAT 
GAGTCCATTT CAAGGCTGCA GGGATGGAAT GAAGCAGTAT TTAAGGAAAA GATTCGCCAG 
ATACAGATTC AAGAAAAATT AATCAGGGAT AGTCAAGGAA AAGTAAAACC TGTTGTTGAT 
AAGAAAAATA AAAAGCTGCT CTGTGGAAAG TGCAAAACCT TTGCATGTTA CACAGCTGAT 
ATAAGAGTGG TGGAGGAATG CCATTTCACT GTGGTTAGAG ATGCTTTCAG GGAGTGCTTC 
GTGACTAAGT TACACCCCAG ACCGAAGAAG TTTGGGAGTT TTGACAAGAA AGCTAAGATC 
TTCTGTGCCA GGAAGGATTG CCTCCATGAC TGGGGAATTC ACGTGAAGTA CAAGACATTC 
GAGATTCCAG TTATAAAGAT CGAAAGTTTT GTGGTGGAAG ATGTTGCGAC TGGAGCTCAG 
ACACTGTATG CAAAGTGGAA GGACTTTAAT TTTGAGAAGA TCCCATTTGA TGCTGCAGAA 
ATGTCCCCTT GCGCTCAGGA CCTCAATCTG CAGGGAGTGG ATGGCCTTGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010837906.1
CDS	964..4017
Translation	MAQVDQGAWGCPAPSFSFDFPSAARLPLQLRLRAGRSPRQISRASRLCLGRAEPSSAEPSRAQAKQGVAGSGAAGMTAEQRRNLHAFRDYVRKILDPTYILSYMTPWFRDDVVQHIQAKKNNKGPMEAASLFLQVLLELQEEGWFRGFLDALQQAGYSGLYEAIESWDFQKLEKLEEYRLLLKRLQPEFKTTINPEDILPEISGCLLNQECEEIIQISSNKGLMAGAEKMVECLLRSDKENWPKTLKLALEKEESKFSELWMVEKGAENVQMKDLEDDEMKTLDVHIVYKEEPESQNLSQNSCSSEVPPTYSPLKPRNYQLELTLPAQKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGRKGKVVIFAVQVPLYEQQKSVFSEHFERFGYKVSGISGETADNISVEQIVENNDIIILTPQILVNSLKDGTIPSLSIFTLMIFDECHNTNKHHPYNMIMFHYLDQKLGGSSDSLPQVIGLTASVGVGDAKNTAEATEYICKLCASLDTAVVTTVRDNLEELEEVVYKPQKFFRKVESRTTDRFKRIISQLMAETEALAKSIFEELGTVTLENLSRIQNRNFGTQKYEQWIIAVQKACMVFQMPDKEEESRICKALFLYTSHLRKYNDALIINEDARMKDALNYLKNFFKNVRAAGFDAIEQDLTQRFEEKLLELEGISMDPSNENAKLKDLCFILQEEYHLNPETRTILFVKTRALVDALKNWIEENPKLSFLKPGILTGRGRTNQTMGMTLPAQKCALDAFRTNRDSKILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNADVIEKEKLNICQEKMMNESISRLQGWNEAVFKEKIRQIQIQEKLIRDSQGKVKPVVDKKNKKLLCGKCKTFACYTADIRVVEECHFTVVRDAFRECFVTKLHPRPKKFGSFDKKAKIFCARKDCLHDWGIHVKYKTFEIPVIKIESFVVEDVATGAQTLYAKWKDFNFEKIPFDAAEMSPCAQDLNLQGVDGLE

Target ORF information:

RefSeq Version	XM_010839604.1
Organism	Bison bison bison
Definition	Bison bison bison DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 (DDX58), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010839604.1

ORF Insert Sequence:

ATGGCGCAGG TTGATCAGGG AGCCTGGGGG TGCCCTGCCC CTAGTTTCAG TTTCGATTTT 
CCCTCCGCAG CCCGTCTTCC CCTGCAGCTC CGCCTCCGCG CTGGGCGCTC GCCTCGGCAA 
ATTTCTCGCG CTTCACGGCT GTGTTTAGGA AGAGCGGAGC CGAGCTCAGC GGAGCCCAGC 
AGAGCACAGG CGAAGCAGGG GGTCGCGGGG AGCGGAGCAG CAGGCATGAC GGCAGAGCAG 
CGGCGGAATT TGCACGCCTT CCGCGACTAT GTCAGGAAGA TCCTGGACCC TACCTACATC 
CTGAGCTACA TGACCCCCTG GTTTAGGGAC GATGTGGTGC AGCACATTCA GGCCAAGAAA 
AACAACAAGG GGCCCATGGA GGCTGCCTCC CTTTTCCTCC AGGTCCTACT GGAGCTCCAG 
GAGGAAGGCT GGTTCCGCGG CTTTTTGGAT GCCCTTCAAC AAGCAGGTTA TTCTGGACTT 
TATGAAGCCA TTGAAAGTTG GGATTTCCAA AAACTTGAAA AATTGGAGGA GTATAGATTA 
CTTTTAAAGC GTTTACAACC AGAATTTAAA ACCACAATCA ATCCAGAAGA TATCCTTCCT 
GAAATATCTG GATGTTTACT TAATCAAGAA TGTGAAGAAA TTATTCAGAT AAGTTCCAAC 
AAGGGGCTGA TGGCAGGTGC AGAGAAAATG GTTGAATGCC TTCTCAGATC AGACAAGGAG 
AACTGGCCCA AAACTTTGAA ACTTGCATTG GAGAAAGAAG AGAGCAAGTT CAGCGAACTG 
TGGATGGTAG AGAAAGGTGC AGAAAATGTT CAAATGAAAG ATCTTGAGGA TGATGAAATG 
AAAACTTTGG ATGTACACAT TGTCTACAAA GAAGAACCAG AAAGCCAGAA TCTTAGCCAG 
AATTCCTGTT CTTCAGAAGT GCCTCCTACT TATAGCCCAT TGAAACCAAG AAATTACCAA 
CTAGAGCTCA CTTTACCTGC TCAGAAGGGA AAGAATACAA TAATATGTGC TCCTACTGGT 
TGTGGAAAAA CCTTTGTTTC CCTTCTTATA TGCGAACATC ATCTTAAGAA ATTTCCACAA 
GGACGAAAGG GGAAAGTTGT CATTTTTGCT GTTCAAGTCC CACTGTATGA ACAGCAGAAA 
TCTGTGTTCT CAGAACATTT TGAAAGATTT GGGTACAAAG TTTCAGGCAT TTCTGGAGAG 
ACAGCTGACA ATATCTCTGT GGAACAGATT GTTGAGAACA ATGACATCAT CATTTTAACA 
CCACAGATTC TTGTGAACAG CCTTAAAGAT GGGACTATTC CATCACTCTC CATCTTTACT 
TTGATGATAT TTGATGAATG TCACAACACC AATAAACACC ATCCATATAA TATGATCATG 
TTTCATTATC TGGATCAGAA ACTGGGAGGA TCTTCAGACT CATTGCCCCA GGTCATTGGG 
CTGACTGCCT CAGTTGGCGT TGGAGATGCC AAAAACACAG CCGAAGCCAC AGAATATATC 
TGCAAACTGT GTGCTTCTCT CGACACAGCA GTGGTAACAA CAGTCAGAGA CAACTTGGAG 
GAACTGGAGG AGGTTGTTTA CAAGCCCCAG AAGTTTTTCA GGAAAGTGGA ATCACGGACT 
ACAGACAGAT TTAAACGCAT CATATCTCAG CTGATGGCAG AGACAGAGGC TCTAGCAAAG 
AGTATCTTTG AAGAACTTGG GACTGTAACT CTTGAAAACT TATCTCGAAT TCAAAATAGG 
AATTTTGGGA CACAGAAATA TGAACAGTGG ATTATTGCAG TTCAGAAAGC ATGCATGGTG 
TTTCAGATGC CAGACAAAGA GGAAGAAAGC AGGATTTGTA AAGCCCTGTT TTTGTACACG 
TCACATTTGC GGAAATACAA TGATGCCCTC ATTATCAATG AGGATGCACG GATGAAAGAT 
GCTCTGAATT ACTTGAAAAA CTTCTTCAAA AATGTCCGGG CAGCAGGATT TGATGCAATT 
GAGCAAGATC TCACTCAGAG ATTTGAAGAA AAGCTGTTGG AACTAGAAGG TATTTCCATG 
GATCCCAGCA ATGAGAACGC TAAACTCAAA GATCTCTGCT TCATCTTGCA AGAGGAATAC 
CACTTAAACC CAGAGACCAG AACCATCCTC TTTGTGAAAA CCCGAGCACT GGTGGATGCC 
TTAAAGAACT GGATTGAAGA AAATCCTAAA CTCAGCTTTC TAAAACCTGG CATATTGACT 
GGACGTGGCA GAACAAATCA GACAATGGGA ATGACGCTCC CAGCACAGAA GTGTGCACTG 
GATGCATTCC GAACCAACAG AGACAGCAAG ATTCTAATTG CTACCTCGGT CGCCGATGAA 
GGCATTGACA TTGCTCAGTG CAATCTGGTC ATCCTCTATG AGTATGTGGG CAATGTCATC 
AAAATGATCC AGACCAGAGG CAGAGGAAGA GCAAGAGGGA GCAAGTGCTT CCTTCTGACT 
AGTAATGCTG ATGTAATCGA AAAAGAAAAA CTGAACATCT GCCAAGAAAA AATGATGAAT 
GAGTCCATTT CAAGGCTGCA GGGATGGAAT GAAGCAGTAT TTAAGGAAAA GATTCGCCAG 
ATACAGATTC AAGAAAAATT AATCAGGGAT AGTCAAGGAA AAGTAAAACC TGTTGTTGAT 
AAGAAAAATA AAAAGCTGCT CTGTGGAAAG TGCAAAACCT TTGCATGTTA CACAGCTGAT 
ATAAGAGTGG TGGAGGAATG CCATTTCACT GTGGTTAGAG ATGCTTTCAG GGAGTGCTTC 
GTGACTAAGT TACACCCCAG ACCGAAGAAG TTTGGGAGTT TTGACAAGAA AGCTAAGATC 
TTCTGTGCCA GGAAGGATTG CCTCCATGAC TGGGGAATTC ACGTGAAGTA CAAGACATTC 
GAGATTCCAG TTATAAAGAT CGAAAGTTTT GTGGTGGAAG ATGTTGCGAC TGGAGCTCAG 
ACACTGTATG CAAAGTGGAA GGACTTTAAT TTTGAGAAGA TCCCATTTGA TGCTGCAGAA 
ATGTCCCCTT GCGCTCAGGA CCTCAATCTG CAGGGAGTGG ATGGCCTTGA ATGA