ANKLE2 cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKLE2
Entrez Gene ID	104638296
Full Name	ankyrin repeat and LEM domain containing 2
Gene Type	protein-coding
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010306060.1	XP_010304362.1	ankyrin repeat and LEM domain-containing protein 2

Pathways from BioSystems
Gene Ontology

The following ANKLE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKLE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa188337	XM_010306060.1 Latest version!	Balearica regulorum gibbericeps ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKLE2 ( XM_010306060.1 ) cDNA ORF clone, Balearica regulorum gibbericeps(East African grey crowned-crane) -> XP_010304362.1 Balearica regulorum gibbericeps ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

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CloneID	OBa188337
Clone ID Related Accession (Same CDS sequence)	XM_010306060.1
Accession Version	XM_010306060.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-17
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Product	ankyrin repeat and LEM domain-containing protein 2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010765042.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Balearica regulorum gibbericeps Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA TATTATCCCG ATTGAAGCAG CTGTCCCCTG ATGAGCTTAG AGAAGAAATT 
GTAAGAGCAG GACTGAAATG TGGGCCCATT ACCTCGACTA CTAGGTTTAT TTTTGAAAAA 
AAATTGGCTC AGGCATTGTT GGAGCAGCAA GGAGTGTTGG AGGAAAAAGG ATCTGCTCTG 
TCAGAGGAGG CTGCTGGAAA TGTCCCATCG GATAGCTGCC GACCAGAAGC ACAAAAGGCT 
TTGAAAACTA CAGCAGTGAA TCACAACGGT CATGGAAGTG TTTCTGAAGA GGCAGACTTT 
GGGTACTGCA TAGGTCTGAA CCCTCCAGAA GAAGATGCTG TGATGCATAT GAACTGTTCA 
GCTCCAGTCT GCGGAGCAGG ATGTGTTGAT TCACAAATTA GTACTCAGAC ACCCTCCAGA 
GATCCTCCAC TGTTCTATGG TGTTTGTCCA GTTTACGACG ACATATTGGC AAGGAATGAG 
AAAATACATG TTTATGAAGA TAAAAAGGAA GCCCTCCAAG CTGTTAAGAT GATTAAGGGT 
TCTCGTTTTA AAGCTTTTTC AAACAGAGAG GATGCTGAGA AATTTGCTAA AGGAATCTGT 
GATTATTTCC CATCTCCAAG CAAGTCCTCT TTATGTTTGT CTCCAGTGAA AATGGGATCG 
TTTAACAGAG ATGGCTTATG TTCCTCTGAG ACTGAAACTG CTAATAAGGA GAGAGCCAAC 
AGTTATAAAA GTCCACGTAC TCAAGATCTT ACTGCTAAAC TTCGGAAAGC TGTCGAGAAA 
GGAGATACAG CAACATTTTC AGAACTTATT TGGAGTAACC CTCGCTATCT AATTGGGTCA 
GGAGACAATC CAACAGTTGT ACAGGAAGGG TGTAGGTACA ATGTCATGCA TGTTGCTGCC 
AAGGAGAATC AACCTAGTAT CTGCCAGTTA TTACTGGACA CTTTGGAAAA TCCTGAATTT 
ATGCGGCTTA TGTATCCAGA TGATAATGAT ATAATGTTGA AAAATCGCAT CCAATATATT 
GTTGACCTTT ACCTTAATAC ACCAGATAAA ATGGGATTTG ATACTCCATT GCATTTTGCC 
TGCAAGTTTG GGAATCTGGA CGTTGTTAAT GTGCTTACCT CACACCCAGC TATTGTGAAA 
AATCCAAGAA ACAAATATGA TCAAACTCCA GCAGAAGTAG TTTGTGAAAG AAGCAAGAAT 
AAATCTGCAG AATTGAAAGA AAAACTAAGA GAGTACTTGA AAGGTCGATA CTATGTACCA 
CTCTTGAGAG CAGAAGACAA TTCCTCAGCT CCTGTAATTG GTGTGCCATG GTCACCCGAT 
CAGACAGATG ATAGCCCTCA AAGAACATTA TCTAAATACA CTGGCAGCCC CAAAGATCCA 
GTGTTGTCAA TAAGAGCTTT TGCAGGCCCC ATGAGCCCCT CAAAGGCTGA AGAATTCCGC 
AGGCTTTGGA AGACACCACC TCGAGAGCGA GCTGGCTTCT TTCACAATGT CAGGAAATCT 
GATCTGGAGA GAGGTGTTGA AAGAGTTGGA AGGGAGTTAG CTCATGAACT GGGGTTCCCG 
TGGGTTGAAT ACTGGGAATT TCTGGGCTGT TTTGTTGATC TGTCTTCCCA GGAGGGGTTG 
CGAAAATTAG AAGAGTACCT GAGTCATCGG GAAATGAGCG AAAAGGCTCA GGAAGAAACA 
GGGGAAAATG AAACCTGCAA TAGATACAAA ACTCCACATC CTTCTGGTAA GAGTAAAAAG 
TGCTGCAATT CTATTTCTGT TGGAGCATTT TTGGATGAGG ATGACGATGA TATGAGCTTA 
GAAGAAATTA AAAACAGACA AAATGCAGCA CGGAATATCA GCCAACCTAT TGTGTCCAAA 
GAACCTAACA TTGATGCTAT AGGGGACGCC GAGTGTGATA TCTTGTCAAT GGAGCGTGCA 
GTAAACATTA TAGAAACATC GGATCGCCCC AGGCACTATG AAAAATTGGC TTCCAGCAAA 
AATGGATTTT GTAATCCTGT GTCCAGTGAA ATGATAATTA GTGACAGAAG GCATTTGCAT 
GACGGAGAAC AATGCCTCAT ATCGCCCGTT TCCAATTTAA TGTCAGAATT TGAAAGCCTG 
TCATTCCAAG AACAAGAGGT TGCAGGAGAA TCAAATAAAA TTGCTGAAAA AACTGAGAAT 
GAGAGGATTC TGGCTGAAGG AACAAGGCAG GTGAGACCAC CCAAGGACCA TCTGGATAAG 
AGCTGCTATG CGGTTCCACT GGCAAGTTTT TCTGAGCCAA GTGTTACAGG TAAACCTGGA 
GAGACCAAGT TAAGGACAGA ACACAAAGTG CCATCAGCAA AAGAGAGACT GTCCATGGAA 
TCTGCAGTTC AGACTAATGA GGCACAACAA CGTCAAGAAC TTTCTTCCCA GAAATTTCTT 
TTGAAGGCTT CACCCACAAA TGACGATTCT AAGAAGTTAT TCCTGCTTGG GGAGCAGCCT 
TCCAAGCTGG ATAGTGATGT CTTAGCAGCT ATAGAAGCAG TAGAGATTGA TCCACAGAAA 
TACCCGACTA TTTACAAGTG GAAACATGCG GTGCAGTCAT ACTCTTCATC TGACAGGCAA 
AGTTGGCCAA GTCCAGCGCT GAAGGCAAAA TTCAAGTCCC AAACCATCGC TGCTGGCCTT 
CCAAATGCTT CAGTGTATTC TACTTCAGGA AGAAACAGTC CCATAACAGG AAGTCCAGGG 
AAATACGGCA ATGCTCCCGC ATTCTCTCCA GACCCCGGGA GTCCTGGGCG CTACAGTCCA 
GCTTGCGTCA ACCATGCTCT GTTAAAACTG CGTTATTTTT CAGAGCCTCC TGCCCACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010304362.1
CDS	1..2820
Translation	MDAILSRLKQLSPDELREEIVRAGLKCGPITSTTRFIFEKKLAQALLEQQGVLEEKGSALSEEAAGNVPSDSCRPEAQKALKTTAVNHNGHGSVSEEADFGYCIGLNPPEEDAVMHMNCSAPVCGAGCVDSQISTQTPSRDPPLFYGVCPVYDDILARNEKIHVYEDKKEALQAVKMIKGSRFKAFSNREDAEKFAKGICDYFPSPSKSSLCLSPVKMGSFNRDGLCSSETETANKERANSYKSPRTQDLTAKLRKAVEKGDTATFSELIWSNPRYLIGSGDNPTVVQEGCRYNVMHVAAKENQPSICQLLLDTLENPEFMRLMYPDDNDIMLKNRIQYIVDLYLNTPDKMGFDTPLHFACKFGNLDVVNVLTSHPAIVKNPRNKYDQTPAEVVCERSKNKSAELKEKLREYLKGRYYVPLLRAEDNSSAPVIGVPWSPDQTDDSPQRTLSKYTGSPKDPVLSIRAFAGPMSPSKAEEFRRLWKTPPRERAGFFHNVRKSDLERGVERVGRELAHELGFPWVEYWEFLGCFVDLSSQEGLRKLEEYLSHREMSEKAQEETGENETCNRYKTPHPSGKSKKCCNSISVGAFLDEDDDDMSLEEIKNRQNAARNISQPIVSKEPNIDAIGDAECDILSMERAVNIIETSDRPRHYEKLASSKNGFCNPVSSEMIISDRRHLHDGEQCLISPVSNLMSEFESLSFQEQEVAGESNKIAEKTENERILAEGTRQVRPPKDHLDKSCYAVPLASFSEPSVTGKPGETKLRTEHKVPSAKERLSMESAVQTNEAQQRQELSSQKFLLKASPTNDDSKKLFLLGEQPSKLDSDVLAAIEAVEIDPQKYPTIYKWKHAVQSYSSSDRQSWPSPALKAKFKSQTIAAGLPNASVYSTSGRNSPITGSPGKYGNAPAFSPDPGSPGRYSPACVNHALLKLRYFSEPPAH

Target ORF information:

RefSeq Version	XM_010306060.1
Organism	Balearica regulorum gibbericeps(East African grey crowned-crane)
Definition	Balearica regulorum gibbericeps ankyrin repeat and LEM domain containing 2 (ANKLE2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010306060.1

ORF Insert Sequence:

ATGGATGCAA TATTATCCCG ATTGAAGCAG CTGTCCCCTG ATGAGCTTAG AGAAGAAATT 
GTAAGAGCAG GACTGAAATG TGGGCCCATT ACCTCGACTA CTAGGTTTAT TTTTGAAAAA 
AAATTGGCTC AGGCATTGTT GGAGCAGCAA GGAGTGTTGG AGGAAAAAGG ATCTGCTCTG 
TCAGAGGAGG CTGCTGGAAA TGTCCCATCG GATAGCTGCC GACCAGAAGC ACAAAAGGCT 
TTGAAAACTA CAGCAGTGAA TCACAACGGT CATGGAAGTG TTTCTGAAGA GGCAGACTTT 
GGGTACTGCA TAGGTCTGAA CCCTCCAGAA GAAGATGCTG TGATGCATAT GAACTGTTCA 
GCTCCAGTCT GCGGAGCAGG ATGTGTTGAT TCACAAATTA GTACTCAGAC ACCCTCCAGA 
GATCCTCCAC TGTTCTATGG TGTTTGTCCA GTTTACGACG ACATATTGGC AAGGAATGAG 
AAAATACATG TTTATGAAGA TAAAAAGGAA GCCCTCCAAG CTGTTAAGAT GATTAAGGGT 
TCTCGTTTTA AAGCTTTTTC AAACAGAGAG GATGCTGAGA AATTTGCTAA AGGAATCTGT 
GATTATTTCC CATCTCCAAG CAAGTCCTCT TTATGTTTGT CTCCAGTGAA AATGGGATCG 
TTTAACAGAG ATGGCTTATG TTCCTCTGAG ACTGAAACTG CTAATAAGGA GAGAGCCAAC 
AGTTATAAAA GTCCACGTAC TCAAGATCTT ACTGCTAAAC TTCGGAAAGC TGTCGAGAAA 
GGAGATACAG CAACATTTTC AGAACTTATT TGGAGTAACC CTCGCTATCT AATTGGGTCA 
GGAGACAATC CAACAGTTGT ACAGGAAGGG TGTAGGTACA ATGTCATGCA TGTTGCTGCC 
AAGGAGAATC AACCTAGTAT CTGCCAGTTA TTACTGGACA CTTTGGAAAA TCCTGAATTT 
ATGCGGCTTA TGTATCCAGA TGATAATGAT ATAATGTTGA AAAATCGCAT CCAATATATT 
GTTGACCTTT ACCTTAATAC ACCAGATAAA ATGGGATTTG ATACTCCATT GCATTTTGCC 
TGCAAGTTTG GGAATCTGGA CGTTGTTAAT GTGCTTACCT CACACCCAGC TATTGTGAAA 
AATCCAAGAA ACAAATATGA TCAAACTCCA GCAGAAGTAG TTTGTGAAAG AAGCAAGAAT 
AAATCTGCAG AATTGAAAGA AAAACTAAGA GAGTACTTGA AAGGTCGATA CTATGTACCA 
CTCTTGAGAG CAGAAGACAA TTCCTCAGCT CCTGTAATTG GTGTGCCATG GTCACCCGAT 
CAGACAGATG ATAGCCCTCA AAGAACATTA TCTAAATACA CTGGCAGCCC CAAAGATCCA 
GTGTTGTCAA TAAGAGCTTT TGCAGGCCCC ATGAGCCCCT CAAAGGCTGA AGAATTCCGC 
AGGCTTTGGA AGACACCACC TCGAGAGCGA GCTGGCTTCT TTCACAATGT CAGGAAATCT 
GATCTGGAGA GAGGTGTTGA AAGAGTTGGA AGGGAGTTAG CTCATGAACT GGGGTTCCCG 
TGGGTTGAAT ACTGGGAATT TCTGGGCTGT TTTGTTGATC TGTCTTCCCA GGAGGGGTTG 
CGAAAATTAG AAGAGTACCT GAGTCATCGG GAAATGAGCG AAAAGGCTCA GGAAGAAACA 
GGGGAAAATG AAACCTGCAA TAGATACAAA ACTCCACATC CTTCTGGTAA GAGTAAAAAG 
TGCTGCAATT CTATTTCTGT TGGAGCATTT TTGGATGAGG ATGACGATGA TATGAGCTTA 
GAAGAAATTA AAAACAGACA AAATGCAGCA CGGAATATCA GCCAACCTAT TGTGTCCAAA 
GAACCTAACA TTGATGCTAT AGGGGACGCC GAGTGTGATA TCTTGTCAAT GGAGCGTGCA 
GTAAACATTA TAGAAACATC GGATCGCCCC AGGCACTATG AAAAATTGGC TTCCAGCAAA 
AATGGATTTT GTAATCCTGT GTCCAGTGAA ATGATAATTA GTGACAGAAG GCATTTGCAT 
GACGGAGAAC AATGCCTCAT ATCGCCCGTT TCCAATTTAA TGTCAGAATT TGAAAGCCTG 
TCATTCCAAG AACAAGAGGT TGCAGGAGAA TCAAATAAAA TTGCTGAAAA AACTGAGAAT 
GAGAGGATTC TGGCTGAAGG AACAAGGCAG GTGAGACCAC CCAAGGACCA TCTGGATAAG 
AGCTGCTATG CGGTTCCACT GGCAAGTTTT TCTGAGCCAA GTGTTACAGG TAAACCTGGA 
GAGACCAAGT TAAGGACAGA ACACAAAGTG CCATCAGCAA AAGAGAGACT GTCCATGGAA 
TCTGCAGTTC AGACTAATGA GGCACAACAA CGTCAAGAAC TTTCTTCCCA GAAATTTCTT 
TTGAAGGCTT CACCCACAAA TGACGATTCT AAGAAGTTAT TCCTGCTTGG GGAGCAGCCT 
TCCAAGCTGG ATAGTGATGT CTTAGCAGCT ATAGAAGCAG TAGAGATTGA TCCACAGAAA 
TACCCGACTA TTTACAAGTG GAAACATGCG GTGCAGTCAT ACTCTTCATC TGACAGGCAA 
AGTTGGCCAA GTCCAGCGCT GAAGGCAAAA TTCAAGTCCC AAACCATCGC TGCTGGCCTT 
CCAAATGCTT CAGTGTATTC TACTTCAGGA AGAAACAGTC CCATAACAGG AAGTCCAGGG 
AAATACGGCA ATGCTCCCGC ATTCTCTCCA GACCCCGGGA GTCCTGGGCG CTACAGTCCA 
GCTTGCGTCA ACCATGCTCT GTTAAAACTG CGTTATTTTT CAGAGCCTCC TGCCCACTAA