LOC106621182 cDNA ORF clone, Bactrocera oleae(olive fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106621182
Entrez Gene ID	106621182
Full Name	probable ATP-dependent RNA helicase DDX27
Gene Type	protein-coding
Organism	Bactrocera oleae(olive fruit fly)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014239923.1	XP_014095398.1	LOW QUALITY PROTEIN: probable ATP-dependent RNA helicase DDX27

Pathways from BioSystems
Gene Ontology

The following LOC106621182 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106621182 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa73888	XM_014239923.1 Latest version!	Bactrocera oleae probable ATP-dependent RNA helicase DDX27 (LOC106621182), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106621182 ( XM_014239923.1 ) cDNA ORF clone, Bactrocera oleae(olive fruit fly) -> XP_014095398.1 Bactrocera oleae probable ATP-dependent RNA helicase DDX27 (LOC106621182), mRNA.

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CloneID	OBa73888
Clone ID Related Accession (Same CDS sequence)	XM_014239923.1
Accession Version	XM_014239923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-20
Organism	Bactrocera oleae(olive fruit fly)
Product	LOW QUALITY PROTEIN: probable ATP-dependent RNA helicase DDX27
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013581617.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bactrocera oleae Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

ATGGGTGCCA CGAAGAATCT GGACTTGATA ACAACTATTG AAGATGATGC AGAGGTAGAA 
AATTTCTCAG AAGATTCCGA TGCTGAAGTG GAGTATCAAC CCACAAAAGT ACGTCACAAA 
AAAGAAACCG AGTTTGAGAC AGGTTTTCAA TTTGTGTCCT CTGTAAAAGA GTACAATAAA 
GATACATGGG ATGATCTCAT GAAATATGTA AAGCGAAAGG CTCGAACCAA GACCGATGAC 
AAAATTGCCG CACGTCTGAA AAAACGTGAA TCTGATGACA AGCCCACAGA AGGCGATGAA 
AGTGGTGACG ATGATGAGAT ACCGCTCTCC GATGATGAAT TGAAACACGA CAATCTACGA 
CTACGTGAGA AGAAATTGTC TAAAAAAAAG AAAAAGAAAT CTGATGATAA CTCGGATGAG 
GAAGAGGAGG AAGGTGAAAA AATGCAGTTT GATGAAGCTA TCGAAAGCAA CGAGTCCATT 
ACATCGTTCT ACCAAATGAA TCTGTCACGT CCGCTAATGC GTGCTATCGG TGTATTGGGT 
TATATATATC CAACTCCCAT ACAGGCTTCG ACAATACCTA TTGCTTTACT GGGTCGCGAT 
ATTTGTGGTT GCGCTGCCAC TGGTACCGGT AAAACAGCGG CTTACATGTT GCCAACACTA 
GAACGTCTTT TATACAGACC AATGAATAAC AAAACAATTA CGCGCGTACT TGTGTTGGTG 
CCGACGCGTG AGTTAGGCGC ACAGGTCTAT CAGGTGACAA AACAGTTGTG TCAATTTACA 
ACAATTGATG TTGGCTTGGC AATTGGTGGT TTAGATGTAA AAGCACAAGA ATTGGTTTTG 
CGACAGAACC CTGATGTTGT TATAGCAACG CCGGGTCGTC TCATCGACCA CATTAAGAAT 
ACGCCTAGTT TTAGCTTGGA TTCAATTGAG GTGCTCATTT TGGACGAAGC AGATCGTATG 
TTAGATGAAT ATTTCGCCGA ACAGATGAAA GAAATTATAA ATTCGTGCGC TAAGACACGG 
CAGACTATGT TGTTCTCGGC AACAATGAGT GAACAAGTCA AAGACTTAGC TGCCGTTTCG 
CTCAGCAAGC CAGTCAAAGT ATTCGTCAAT AATAATCAAC AAGTTGCCTT CAATCTTCGA 
CAAGAATTCA TTCGTATCCG TGAGGATAAA GAGGGTGATC GTGAACCCAT ACTAGCCAGT 
TTAATTTGTC GTACATTCCA CGATCATTGC ATGGTATTTG TTCAAACGAA AAAGCAAGCT 
CATCGTCTTC ACATTTTACT GGGCTTATTA GGCATACGTG CTGGTGAACT GCATGGTAAC 
CTCACGCAAC AACAACGTCT GGAGTCGTTA AAGAAATTCA AAGAAGAACA AATAGACGTA 
CTCATAGCGA CGGACGTGGC GGCTCGTGGT TTGGATATTG TGGGCGTAAA AACCGTCATT 
AACTTTGTCA TGCCTATTAC AACCGAGCAC TATATACATC GAGTCGGTCG TACTGCTCGT 
GCAGGGCGTG CAGGTATTTC GGTATCATTG GCTGGTGAAA AAGAGCGTAA AATCGTTAAA 
GACATTATTA AGAATGCGGA GAACTCGGTG AAGAATCGTA TTATACCACC AGAAATTATT 
GAAAAATATC GCAAGAAACT GATCGCACTC GAACCGGAAA TACAACACAT TTTGGACGAA 
GAGCAAACAG AACGTCAGCT TGCCAAGATG GAGCAGCAGC TTTCAAAAAC GGAACGCAAA 
TTGCAGGGCA CCCAGACAGA GCAACGCGCG TGGTTCCAAT CGCGTCAACA GCGTGAAGAT 
GAAAAGGCGC GTCTAGCCAT TACGCCCCCC GAAGAGAATC AAAAGGCTGG TGCCAATGAA 
AAGAAAGGTG CCGGCAAACG GAAGCGCACT GAATATGGTG GCGAAGGCGA AGATGGACCA 
CCNNNGAAGA AAAAGTCACC AGAGCAATTG GCAAAGGAGC GAGTTATAAA GGAGATAGAA 
AAGGCGTCTT TGGTACGCGC TAAGATGTCA AAGCTGAAGA AACGTCCGGG TAAATTAAGC 
GCAGTAGCTG ATGAGCAAGC CCGGAAACAT GTGAGCGACT ACAATAAACT AAAACCGACA 
AAACGTCGTG GTCAGTCAGC ATTCACGAAC GACTTGACAG ATGTGAGCCG TAGCGGAGCG 
TTGCGTCTGA GATCGGAAGC CAATCGCCAT AAGAAGCTCA CTCAAATAGC GAAAAAGAAG 
AAGACTAGCG GTGTAAAAAT TGCCAATAAA GCTGGCAAAA ATAAGTTCAA TAAAGGCAAG 
AACTTTGGTG GTCCTCGTTT TACAAAAAAG AATAAGAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_014095398.1
CDS	120..2441
Translation	MGATKNLDLITTIEDDAEVENFSEDSDAEVEYQPTKVRHKKETEFETGFQFVSSVKEYNKDTWDDLMKYVKRKARTKTDDKIAARLKKRESDDKPTEGDESGDDDEIPLSDDELKHDNLRLREKKLSKKKKKKSDDNSDEEEEEGEKMQFDEAIESNESITSFYQMNLSRPLMRAIGVLGYIYPTPIQASTIPIALLGRDICGCAATGTGKTAAYMLPTLERLLYRPMNNKTITRVLVLVPTRELGAQVYQVTKQLCQFTTIDVGLAIGGLDVKAQELVLRQNPDVVIATPGRLIDHIKNTPSFSLDSIEVLILDEADRMLDEYFAEQMKEIINSCAKTRQTMLFSATMSEQVKDLAAVSLSKPVKVFVNNNQQVAFNLRQEFIRIREDKEGDREPILASLICRTFHDHCMVFVQTKKQAHRLHILLGLLGIRAGELHGNLTQQQRLESLKKFKEEQIDVLIATDVAARGLDIVGVKTVINFVMPITTEHYIHRVGRTARAGRAGISVSLAGEKERKIVKDIIKNAENSVKNRIIPPEIIEKYRKKLIALEPEIQHILDEEQTERQLAKMEQQLSKTERKLQGTQTEQRAWFQSRQQREDEKARLAITPPEENQKAGANEKKGAGKRKRTEYGGEGEDGPnullnullKKKSPEQLAKERVIKEIEKASLVRAKMSKLKKRPGKLSAVADEQARKHVSDYNKLKPTKRRGQSAFTNDLTDVSRSGALRLRSEANRHKKLTQIAKKKKTSGVKIANKAGKNKFNKGKNFGGPRFTKKNKK

Target ORF information:

RefSeq Version	XM_014239923.1
Organism	Bactrocera oleae(olive fruit fly)
Definition	Bactrocera oleae probable ATP-dependent RNA helicase DDX27 (LOC106621182), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014239923.1

ORF Insert Sequence:

ATGGGTGCCA CGAAGAATCT GGACTTGATA ACAACTATTG AAGATGATGC AGAGGTAGAA 
AATTTCTCAG AAGATTCCGA TGCTGAAGTG GAGTATCAAC CCACAAAAGT ACGTCACAAA 
AAAGAAACCG AGTTTGAGAC AGGTTTTCAA TTTGTGTCCT CTGTAAAAGA GTACAATAAA 
GATACATGGG ATGATCTCAT GAAATATGTA AAGCGAAAGG CTCGAACCAA GACCGATGAC 
AAAATTGCCG CACGTCTGAA AAAACGTGAA TCTGATGACA AGCCCACAGA AGGCGATGAA 
AGTGGTGACG ATGATGAGAT ACCGCTCTCC GATGATGAAT TGAAACACGA CAATCTACGA 
CTACGTGAGA AGAAATTGTC TAAAAAAAAG AAAAAGAAAT CTGATGATAA CTCGGATGAG 
GAAGAGGAGG AAGGTGAAAA AATGCAGTTT GATGAAGCTA TCGAAAGCAA CGAGTCCATT 
ACATCGTTCT ACCAAATGAA TCTGTCACGT CCGCTAATGC GTGCTATCGG TGTATTGGGT 
TATATATATC CAACTCCCAT ACAGGCTTCG ACAATACCTA TTGCTTTACT GGGTCGCGAT 
ATTTGTGGTT GCGCTGCCAC TGGTACCGGT AAAACAGCGG CTTACATGTT GCCAACACTA 
GAACGTCTTT TATACAGACC AATGAATAAC AAAACAATTA CGCGCGTACT TGTGTTGGTG 
CCGACGCGTG AGTTAGGCGC ACAGGTCTAT CAGGTGACAA AACAGTTGTG TCAATTTACA 
ACAATTGATG TTGGCTTGGC AATTGGTGGT TTAGATGTAA AAGCACAAGA ATTGGTTTTG 
CGACAGAACC CTGATGTTGT TATAGCAACG CCGGGTCGTC TCATCGACCA CATTAAGAAT 
ACGCCTAGTT TTAGCTTGGA TTCAATTGAG GTGCTCATTT TGGACGAAGC AGATCGTATG 
TTAGATGAAT ATTTCGCCGA ACAGATGAAA GAAATTATAA ATTCGTGCGC TAAGACACGG 
CAGACTATGT TGTTCTCGGC AACAATGAGT GAACAAGTCA AAGACTTAGC TGCCGTTTCG 
CTCAGCAAGC CAGTCAAAGT ATTCGTCAAT AATAATCAAC AAGTTGCCTT CAATCTTCGA 
CAAGAATTCA TTCGTATCCG TGAGGATAAA GAGGGTGATC GTGAACCCAT ACTAGCCAGT 
TTAATTTGTC GTACATTCCA CGATCATTGC ATGGTATTTG TTCAAACGAA AAAGCAAGCT 
CATCGTCTTC ACATTTTACT GGGCTTATTA GGCATACGTG CTGGTGAACT GCATGGTAAC 
CTCACGCAAC AACAACGTCT GGAGTCGTTA AAGAAATTCA AAGAAGAACA AATAGACGTA 
CTCATAGCGA CGGACGTGGC GGCTCGTGGT TTGGATATTG TGGGCGTAAA AACCGTCATT 
AACTTTGTCA TGCCTATTAC AACCGAGCAC TATATACATC GAGTCGGTCG TACTGCTCGT 
GCAGGGCGTG CAGGTATTTC GGTATCATTG GCTGGTGAAA AAGAGCGTAA AATCGTTAAA 
GACATTATTA AGAATGCGGA GAACTCGGTG AAGAATCGTA TTATACCACC AGAAATTATT 
GAAAAATATC GCAAGAAACT GATCGCACTC GAACCGGAAA TACAACACAT TTTGGACGAA 
GAGCAAACAG AACGTCAGCT TGCCAAGATG GAGCAGCAGC TTTCAAAAAC GGAACGCAAA 
TTGCAGGGCA CCCAGACAGA GCAACGCGCG TGGTTCCAAT CGCGTCAACA GCGTGAAGAT 
GAAAAGGCGC GTCTAGCCAT TACGCCCCCC GAAGAGAATC AAAAGGCTGG TGCCAATGAA 
AAGAAAGGTG CCGGCAAACG GAAGCGCACT GAATATGGTG GCGAAGGCGA AGATGGACCA 
CCNNNGAAGA AAAAGTCACC AGAGCAATTG GCAAAGGAGC GAGTTATAAA GGAGATAGAA 
AAGGCGTCTT TGGTACGCGC TAAGATGTCA AAGCTGAAGA AACGTCCGGG TAAATTAAGC 
GCAGTAGCTG ATGAGCAAGC CCGGAAACAT GTGAGCGACT ACAATAAACT AAAACCGACA 
AAACGTCGTG GTCAGTCAGC ATTCACGAAC GACTTGACAG ATGTGAGCCG TAGCGGAGCG 
TTGCGTCTGA GATCGGAAGC CAATCGCCAT AAGAAGCTCA CTCAAATAGC GAAAAAGAAG 
AAGACTAGCG GTGTAAAAAT TGCCAATAAA GCTGGCAAAA ATAAGTTCAA TAAAGGCAAG 
AACTTTGGTG GTCCTCGTTT TACAAAAAAG AATAAGAAGT AA