LOC106528391 cDNA ORF clone, Austrofundulus limnaeus

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC106528391
Entrez Gene ID	106528391
Full Name	putative nuclease HARBI1
Gene Type	protein-coding
Organism	Austrofundulus limnaeus

mRNA and Protein(s)

mRNA	Protein	Name
XM_014023544.1	XP_013878998.1	putative nuclease HARBI1 isoform X2
XM_014023543.1	XP_013878997.1	putative nuclease HARBI1 isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC106528391 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106528391 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu21100	XM_014023543.1 Latest version!	Austrofundulus limnaeus putative nuclease HARBI1 (LOC106528391), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OAu21101	XM_014023544.1 Latest version!	Austrofundulus limnaeus putative nuclease HARBI1 (LOC106528391), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC106528391 ( XM_014023543.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013878997.1 Austrofundulus limnaeus putative nuclease HARBI1 (LOC106528391), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAu21100
Clone ID Related Accession (Same CDS sequence)	XM_014023543.1
Accession Version	XM_014023543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	putative nuclease HARBI1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952778.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGG ACGAGCAGCT GCGGTCCTTA ATGTTTCTTA TGACTTATCT GCTGCTGAAG 
CGACGCAGGG ACGTAAACAG CGCGAACATC CAGAGGCGCG ATGAAATCCA GAGACGAGTC 
CGACACCGAC AGTACTTCTT CCAGAGACAA AGGAGGATGC TTATGATGAT GATCGCGGGA 
GGCATCCGCT CCAACGTCCG CATCCGCACA CGTCCCTGGA CCACAACTCC TCGCTCGGAC 
TGGTGGGAGC GGGTGGTGAT GACCGAGTTC CAGCCCTCCG ACTGGCTGGA TAAGTTCCGG 
ATGAGCCGAG AGACCTTCTT TTACCTCTGC GACAAGCTGA GGCCCCGCCT GGCCCGCCAG 
GACACCAGCT TCCGCCTGGC CCTGCCGGTG GAGAAGCGCG TCGCCGTGGC CCTGTGGCGG 
CTGGCGTCCA ACGTGGAGTA CCGCACCATC AGCTCCCTGT TCGGCGTAGG GAAGTCCACC 
GTGTGCAGGT GCGTTCGAGA CATGTGTCAC GCCATCGTGG CGCTCCTCAG CTCCATCTAC 
CTGCGGCCCC CCAGCGAGCA GGAGCTGGAG GACTCCGCTC AGCTCTTCCT GGCCTACTGG 
GGCTTCCCCC ACTGCGTCGC TGCCGTAGCC ACACTTCACA CCGCCATCAT CACCCCGTCG 
AGCAACGCGT CCGACTACGC CAACCCTGCC GGCTGGCTCT CGGTTTTGTC TCAGGTAGAG 
AGAACTTCAG TTGAAGCCTC CGCTGCTGGT GCTCGCTCTC ACGCTGCCTG TTTTTGTGGG 
CTCCCTCAGG TGGCTGTCAG CGCCCGCGGC CTCTTCTGGG ACGTGTGTGC CAGCTTCCCC 
GGTGGAACAG ATCCAGCTGA CATCTTCCAG AACTCTTCAC TGTGGGCCAC AGCTGCTGAG 
GGGGGGCTGT CTCCTGCCCC GACACCTATT TTCATGGGGC GACCCCTCAG GTATGTGTTG 
CTCGGGGAGG CTTGCTACCC GCTCCAGAGC TGGCTGATGA AGGCCTACCC CGAGGAGCGG 
AGCCGCAGGG CGAGTCGCAG CACGCTGACG GAGCCACAGC AGCTGTTCAA CTGGCAGCTC 
CGCCGGGCGC TGCGCGTGTC CGAGGAGGCG CTGCTGAAGC TGAGGGCGCG CTGGCAGTGT 
CTGAGCAAGA GGAACGACTG CGGGCTGGAC GTGGTGCCCA CCATGATCCT GGCTTGCTGC 
ATCCTGCACA ACATGTGCGA GTCCCACGGA GACACCTTTA AAGCCGACTG GCAGGTCGAG 
GTCTCCGAGG CGGAGAGTCC GCAGCCCACT CACAAGACGC TCCTCTCAAC CAGCATGGAC 
CAAAGTAATG CAGAGGAGGT CCGAGGCCTC TTCTGTGACT ACTTCCAACT GCAGAACCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013878997.1
CDS	119..1501
Translation	MGEDEQLRSLMFLMTYLLLKRRRDVNSANIQRRDEIQRRVRHRQYFFQRQRRMLMMMIAGGIRSNVRIRTRPWTTTPRSDWWERVVMTEFQPSDWLDKFRMSRETFFYLCDKLRPRLARQDTSFRLALPVEKRVAVALWRLASNVEYRTISSLFGVGKSTVCRCVRDMCHAIVALLSSIYLRPPSEQELEDSAQLFLAYWGFPHCVAAVATLHTAIITPSSNASDYANPAGWLSVLSQVERTSVEASAAGARSHAACFCGLPQVAVSARGLFWDVCASFPGGTDPADIFQNSSLWATAAEGGLSPAPTPIFMGRPLRYVLLGEACYPLQSWLMKAYPEERSRRASRSTLTEPQQLFNWQLRRALRVSEEALLKLRARWQCLSKRNDCGLDVVPTMILACCILHNMCESHGDTFKADWQVEVSEAESPQPTHKTLLSTSMDQSNAEEVRGLFCDYFQLQNH

Target ORF information:

RefSeq Version	XM_014023543.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus putative nuclease HARBI1 (LOC106528391), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014023543.1

ORF Insert Sequence:

ATGGGAGAGG ACGAGCAGCT GCGGTCCTTA ATGTTTCTTA TGACTTATCT GCTGCTGAAG 
CGACGCAGGG ACGTAAACAG CGCGAACATC CAGAGGCGCG ATGAAATCCA GAGACGAGTC 
CGACACCGAC AGTACTTCTT CCAGAGACAA AGGAGGATGC TTATGATGAT GATCGCGGGA 
GGCATCCGCT CCAACGTCCG CATCCGCACA CGTCCCTGGA CCACAACTCC TCGCTCGGAC 
TGGTGGGAGC GGGTGGTGAT GACCGAGTTC CAGCCCTCCG ACTGGCTGGA TAAGTTCCGG 
ATGAGCCGAG AGACCTTCTT TTACCTCTGC GACAAGCTGA GGCCCCGCCT GGCCCGCCAG 
GACACCAGCT TCCGCCTGGC CCTGCCGGTG GAGAAGCGCG TCGCCGTGGC CCTGTGGCGG 
CTGGCGTCCA ACGTGGAGTA CCGCACCATC AGCTCCCTGT TCGGCGTAGG GAAGTCCACC 
GTGTGCAGGT GCGTTCGAGA CATGTGTCAC GCCATCGTGG CGCTCCTCAG CTCCATCTAC 
CTGCGGCCCC CCAGCGAGCA GGAGCTGGAG GACTCCGCTC AGCTCTTCCT GGCCTACTGG 
GGCTTCCCCC ACTGCGTCGC TGCCGTAGCC ACACTTCACA CCGCCATCAT CACCCCGTCG 
AGCAACGCGT CCGACTACGC CAACCCTGCC GGCTGGCTCT CGGTTTTGTC TCAGGTAGAG 
AGAACTTCAG TTGAAGCCTC CGCTGCTGGT GCTCGCTCTC ACGCTGCCTG TTTTTGTGGG 
CTCCCTCAGG TGGCTGTCAG CGCCCGCGGC CTCTTCTGGG ACGTGTGTGC CAGCTTCCCC 
GGTGGAACAG ATCCAGCTGA CATCTTCCAG AACTCTTCAC TGTGGGCCAC AGCTGCTGAG 
GGGGGGCTGT CTCCTGCCCC GACACCTATT TTCATGGGGC GACCCCTCAG GTATGTGTTG 
CTCGGGGAGG CTTGCTACCC GCTCCAGAGC TGGCTGATGA AGGCCTACCC CGAGGAGCGG 
AGCCGCAGGG CGAGTCGCAG CACGCTGACG GAGCCACAGC AGCTGTTCAA CTGGCAGCTC 
CGCCGGGCGC TGCGCGTGTC CGAGGAGGCG CTGCTGAAGC TGAGGGCGCG CTGGCAGTGT 
CTGAGCAAGA GGAACGACTG CGGGCTGGAC GTGGTGCCCA CCATGATCCT GGCTTGCTGC 
ATCCTGCACA ACATGTGCGA GTCCCACGGA GACACCTTTA AAGCCGACTG GCAGGTCGAG 
GTCTCCGAGG CGGAGAGTCC GCAGCCCACT CACAAGACGC TCCTCTCAAC CAGCATGGAC 
CAAAGTAATG CAGAGGAGGT CCGAGGCCTC TTCTGTGACT ACTTCCAACT GCAGAACCAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC106528391 ( XM_014023544.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013878998.1 Austrofundulus limnaeus putative nuclease HARBI1 (LOC106528391), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAu21101
Clone ID Related Accession (Same CDS sequence)	XM_014023544.1
Accession Version	XM_014023544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1308bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	putative nuclease HARBI1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952778.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGG ACGAGCAGCT GCGGTCCTTA ATGTTTCTTA TGACTTATCT GCTGCTGAAG 
CGACGCAGGG ACGTAAACAG CGCGAACATC CAGAGGCGCG ATGAAATCCA GAGACGAGTC 
CGACACCGAC AGTACTTCTT CCAGAGACAA AGGAGGATGC TTATGATGAT GATCGCGGGA 
GGCATCCGCT CCAACGTCCG CATCCGCACA CGTCCCTGGA CCACAACTCC TCGCTCGGAC 
TGGTGGGAGC GGGTGGTGAT GACCGAGTTC CAGCCCTCCG ACTGGCTGGA TAAGTTCCGG 
ATGAGCCGAG AGACCTTCTT TTACCTCTGC GACAAGCTGA GGCCCCGCCT GGCCCGCCAG 
GACACCAGCT TCCGCCTGGC CCTGCCGGTG GAGAAGCGCG TCGCCGTGGC CCTGTGGCGG 
CTGGCGTCCA ACGTGGAGTA CCGCACCATC AGCTCCCTGT TCGGCGTAGG GAAGTCCACC 
GTGTGCAGGT GCGTTCGAGA CATGTGTCAC GCCATCGTGG CGCTCCTCAG CTCCATCTAC 
CTGCGGCCCC CCAGCGAGCA GGAGCTGGAG GACTCCGCTC AGCTCTTCCT GGCCTACTGG 
GGCTTCCCCC ACTGCGTCGC TGCCGTAGCC ACACTTCACA CCGCCATCAT CACCCCGTCG 
AGCAACGCGT CCGACTACGC CAACCCTGCC GGCTGGCTCT CGGTTTTGTC TCAGGTGGCT 
GTCAGCGCCC GCGGCCTCTT CTGGGACGTG TGTGCCAGCT TCCCCGGTGG AACAGATCCA 
GCTGACATCT TCCAGAACTC TTCACTGTGG GCCACAGCTG CTGAGGGGGG GCTGTCTCCT 
GCCCCGACAC CTATTTTCAT GGGGCGACCC CTCAGGTATG TGTTGCTCGG GGAGGCTTGC 
TACCCGCTCC AGAGCTGGCT GATGAAGGCC TACCCCGAGG AGCGGAGCCG CAGGGCGAGT 
CGCAGCACGC TGACGGAGCC ACAGCAGCTG TTCAACTGGC AGCTCCGCCG GGCGCTGCGC 
GTGTCCGAGG AGGCGCTGCT GAAGCTGAGG GCGCGCTGGC AGTGTCTGAG CAAGAGGAAC 
GACTGCGGGC TGGACGTGGT GCCCACCATG ATCCTGGCTT GCTGCATCCT GCACAACATG 
TGCGAGTCCC ACGGAGACAC CTTTAAAGCC GACTGGCAGG TCGAGGTCTC CGAGGCGGAG 
AGTCCGCAGC CCACTCACAA GACGCTCCTC TCAACCAGCA TGGACCAAAG TAATGCAGAG 
GAGGTCCGAG GCCTCTTCTG TGACTACTTC CAACTGCAGA ACCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013878998.1
CDS	117..1424
Translation	MGEDEQLRSLMFLMTYLLLKRRRDVNSANIQRRDEIQRRVRHRQYFFQRQRRMLMMMIAGGIRSNVRIRTRPWTTTPRSDWWERVVMTEFQPSDWLDKFRMSRETFFYLCDKLRPRLARQDTSFRLALPVEKRVAVALWRLASNVEYRTISSLFGVGKSTVCRCVRDMCHAIVALLSSIYLRPPSEQELEDSAQLFLAYWGFPHCVAAVATLHTAIITPSSNASDYANPAGWLSVLSQVAVSARGLFWDVCASFPGGTDPADIFQNSSLWATAAEGGLSPAPTPIFMGRPLRYVLLGEACYPLQSWLMKAYPEERSRRASRSTLTEPQQLFNWQLRRALRVSEEALLKLRARWQCLSKRNDCGLDVVPTMILACCILHNMCESHGDTFKADWQVEVSEAESPQPTHKTLLSTSMDQSNAEEVRGLFCDYFQLQNH

Target ORF information:

RefSeq Version	XM_014023544.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus putative nuclease HARBI1 (LOC106528391), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014023544.1

ORF Insert Sequence:

ATGGGAGAGG ACGAGCAGCT GCGGTCCTTA ATGTTTCTTA TGACTTATCT GCTGCTGAAG 
CGACGCAGGG ACGTAAACAG CGCGAACATC CAGAGGCGCG ATGAAATCCA GAGACGAGTC 
CGACACCGAC AGTACTTCTT CCAGAGACAA AGGAGGATGC TTATGATGAT GATCGCGGGA 
GGCATCCGCT CCAACGTCCG CATCCGCACA CGTCCCTGGA CCACAACTCC TCGCTCGGAC 
TGGTGGGAGC GGGTGGTGAT GACCGAGTTC CAGCCCTCCG ACTGGCTGGA TAAGTTCCGG 
ATGAGCCGAG AGACCTTCTT TTACCTCTGC GACAAGCTGA GGCCCCGCCT GGCCCGCCAG 
GACACCAGCT TCCGCCTGGC CCTGCCGGTG GAGAAGCGCG TCGCCGTGGC CCTGTGGCGG 
CTGGCGTCCA ACGTGGAGTA CCGCACCATC AGCTCCCTGT TCGGCGTAGG GAAGTCCACC 
GTGTGCAGGT GCGTTCGAGA CATGTGTCAC GCCATCGTGG CGCTCCTCAG CTCCATCTAC 
CTGCGGCCCC CCAGCGAGCA GGAGCTGGAG GACTCCGCTC AGCTCTTCCT GGCCTACTGG 
GGCTTCCCCC ACTGCGTCGC TGCCGTAGCC ACACTTCACA CCGCCATCAT CACCCCGTCG 
AGCAACGCGT CCGACTACGC CAACCCTGCC GGCTGGCTCT CGGTTTTGTC TCAGGTGGCT 
GTCAGCGCCC GCGGCCTCTT CTGGGACGTG TGTGCCAGCT TCCCCGGTGG AACAGATCCA 
GCTGACATCT TCCAGAACTC TTCACTGTGG GCCACAGCTG CTGAGGGGGG GCTGTCTCCT 
GCCCCGACAC CTATTTTCAT GGGGCGACCC CTCAGGTATG TGTTGCTCGG GGAGGCTTGC 
TACCCGCTCC AGAGCTGGCT GATGAAGGCC TACCCCGAGG AGCGGAGCCG CAGGGCGAGT 
CGCAGCACGC TGACGGAGCC ACAGCAGCTG TTCAACTGGC AGCTCCGCCG GGCGCTGCGC 
GTGTCCGAGG AGGCGCTGCT GAAGCTGAGG GCGCGCTGGC AGTGTCTGAG CAAGAGGAAC 
GACTGCGGGC TGGACGTGGT GCCCACCATG ATCCTGGCTT GCTGCATCCT GCACAACATG 
TGCGAGTCCC ACGGAGACAC CTTTAAAGCC GACTGGCAGG TCGAGGTCTC CGAGGCGGAG 
AGTCCGCAGC CCACTCACAA GACGCTCCTC TCAACCAGCA TGGACCAAAG TAATGCAGAG 
GAGGTCCGAG GCCTCTTCTG TGACTACTTC CAACTGCAGA ACCACTGA