retsat cDNA ORF clone, Austrofundulus limnaeus

Gene
Clones
gRNAs

Summary

Gene Symbol	retsat
Entrez Gene ID	106527571
Full Name	retinol saturase
Gene Type	protein-coding
Organism	Austrofundulus limnaeus

mRNA and Protein(s)

mRNA	Protein	Name
XM_014022496.1	XP_013877950.1	all-trans-retinol 13,14-reductase isoform X1
XM_014022504.1	XP_013877958.1	all-trans-retinol 13,14-reductase isoform X2

Pathways from BioSystems
Gene Ontology

The following retsat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the retsat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu02012	XM_014022504.1 Latest version!	Austrofundulus limnaeus retinol saturase (all-trans-retinol 13,14-reductase) (retsat), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OAu02011	XM_014022496.1 Latest version!	Austrofundulus limnaeus retinol saturase (all-trans-retinol 13,14-reductase) (retsat), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

retsat ( XM_014022504.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013877958.1 Austrofundulus limnaeus retinol saturase (all-trans-retinol 13,14-reductase) (retsat), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAu02012
Clone ID Related Accession (Same CDS sequence)	XM_014022504.1
Accession Version	XM_014022504.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1440bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	all-trans-retinol 13,14-reductase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952406.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGGACC AGATGACCAA TGGGCAGCTG CAGTGGGAGC CTCTGGAGAA CCCGTTTGAC 
CACGTCCTGC TGGGACCTCC AGAAAACCGC CGCAGGTACC CCATCTACAG CGGCAAGACT 
CGCTTCCCCG AAGAGCTGAA GAAGTGTTTC CCCGGAGAGG AGAAAGCCAT CGACGAGTAC 
CTGCGGCTGG CCAAGAAAAC TAAAAACGGA GTTTGGCTCA TGGCTATGCT GAAGCTCCTT 
CCAGTCTTTC TGACCAAGTT CCTGATCTGC ACCGGCCTCG TCGAACGTCT GTCTTTCTTC 
TTCAAAATGG CACCCCGCAG CCTGACAGAC GTGGTCAACG AGCTGACACA GAACCAGGAC 
CTCAGAGCCG TATTCTCCTA CATTGTTGGC ACTTACGGAA AGACTCCTAA AGAGGCCAGC 
TTTGCCATGC ACAGTCTGCT GGTGACTCAC TACCTGGACG GCGCCTGGTA CCCTAAAGGC 
GGCTCCAGTG AAATCGCATA CCACATGATC CCCATTGTGG AGAAGGCGGG AGGCGCTGTT 
TTGGTCCGAG CCCCGGTGAA CCGGATCCTG TTCAATGATG CCAAAGAAGC TTACGGTGTT 
AGTGTGTTGA AGGGTCAGGA GGAAGTGCAC ATCCGCGCCC CCATGATCGT TTCCGATGCC 
GGCATCTTCA ACACGTACGA GAAGCTGCTG CCCGCAGAGC TCCAAGCCCT GCCAGCCATC 
CAGGAGCAGC TCAGCATGGT GAAACACGGT GATGGCGGCT TGAGCATCTT TATGGGTCTG 
AACGGAACCA AGGAGGAGCT GGGCCTGAAA GCGGACAACT ACTGGATCTT TGCTGAGAAC 
AATTTGGACA AACTGCTGGA GGACTATAAT AGTAAAGCGA GAGAACAGGC TGCTCAGAAG 
ATCCCTCTGC TGTTTGTCGC CTGTCCATCA GCTAAAGATC CAACCTGGGA GGAGAGATCA 
CCGGGAAAGT CAACTTTGAG TCTGGTCAGT TTTGCTAAAT ACGAGTGGTT TGAGGAYTGG 
AAGGACGACA AAGTGACGAA CCGCGGGCCC GACTACAAAG AGCTGAAACA AATGTTTATC 
GACTCGGCCC TGGAGGTGGT GATGGACGTG TTTCCTAAGA TCACCAGAGA CAAGATTGAG 
TACATTGATG CTGGAACCCC CGTCACAAAC ACGCACTATA TCGCAGCCCC GAAAGGTGAG 
ATCTACGGTG TGGATCATGA CATCGGTCGG TTCAGGCCTG AACTCAACGC TACGATAAGA 
CCTCAGACCC CTCTGAAGAA CCTCTACCTG ACAGGTCAGG ATATGTTTCT GTGTGGTTTC 
GCCGGCGCCC TGGCCGGAGC GCTCACCTGT GGCTCGGTCA TTCTTAACCG CAACCTCCAT 
CTGGACGCCA TCGCCTTGGC CAAAAGAGCA AAACATTTTA ACAAGCAGAA AAAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013877958.1
CDS	139..1578
Translation	MLDQMTNGQLQWEPLENPFDHVLLGPPENRRRYPIYSGKTRFPEELKKCFPGEEKAIDEYLRLAKKTKNGVWLMAMLKLLPVFLTKFLICTGLVERLSFFFKMAPRSLTDVVNELTQNQDLRAVFSYIVGTYGKTPKEASFAMHSLLVTHYLDGAWYPKGGSSEIAYHMIPIVEKAGGAVLVRAPVNRILFNDAKEAYGVSVLKGQEEVHIRAPMIVSDAGIFNTYEKLLPAELQALPAIQEQLSMVKHGDGGLSIFMGLNGTKEELGLKADNYWIFAENNLDKLLEDYNSKAREQAAQKIPLLFVACPSAKDPTWEERSPGKSTLSLVSFAKYEWFEnullWKDDKVTNRGPDYKELKQMFIDSALEVVMDVFPKITRDKIEYIDAGTPVTNTHYIAAPKGEIYGVDHDIGRFRPELNATIRPQTPLKNLYLTGQDMFLCGFAGALAGALTCGSVILNRNLHLDAIALAKRAKHFNKQKKE

Target ORF information:

RefSeq Version	XM_014022504.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus retinol saturase (all-trans-retinol 13,14-reductase) (retsat), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014022504.1

ORF Insert Sequence:

ATGTTGGACC AGATGACCAA TGGGCAGCTG CAGTGGGAGC CTCTGGAGAA CCCGTTTGAC 
CACGTCCTGC TGGGACCTCC AGAAAACCGC CGCAGGTACC CCATCTACAG CGGCAAGACT 
CGCTTCCCCG AAGAGCTGAA GAAGTGTTTC CCCGGAGAGG AGAAAGCCAT CGACGAGTAC 
CTGCGGCTGG CCAAGAAAAC TAAAAACGGA GTTTGGCTCA TGGCTATGCT GAAGCTCCTT 
CCAGTCTTTC TGACCAAGTT CCTGATCTGC ACCGGCCTCG TCGAACGTCT GTCTTTCTTC 
TTCAAAATGG CACCCCGCAG CCTGACAGAC GTGGTCAACG AGCTGACACA GAACCAGGAC 
CTCAGAGCCG TATTCTCCTA CATTGTTGGC ACTTACGGAA AGACTCCTAA AGAGGCCAGC 
TTTGCCATGC ACAGTCTGCT GGTGACTCAC TACCTGGACG GCGCCTGGTA CCCTAAAGGC 
GGCTCCAGTG AAATCGCATA CCACATGATC CCCATTGTGG AGAAGGCGGG AGGCGCTGTT 
TTGGTCCGAG CCCCGGTGAA CCGGATCCTG TTCAATGATG CCAAAGAAGC TTACGGTGTT 
AGTGTGTTGA AGGGTCAGGA GGAAGTGCAC ATCCGCGCCC CCATGATCGT TTCCGATGCC 
GGCATCTTCA ACACGTACGA GAAGCTGCTG CCCGCAGAGC TCCAAGCCCT GCCAGCCATC 
CAGGAGCAGC TCAGCATGGT GAAACACGGT GATGGCGGCT TGAGCATCTT TATGGGTCTG 
AACGGAACCA AGGAGGAGCT GGGCCTGAAA GCGGACAACT ACTGGATCTT TGCTGAGAAC 
AATTTGGACA AACTGCTGGA GGACTATAAT AGTAAAGCGA GAGAACAGGC TGCTCAGAAG 
ATCCCTCTGC TGTTTGTCGC CTGTCCATCA GCTAAAGATC CAACCTGGGA GGAGAGATCA 
CCGGGAAAGT CAACTTTGAG TCTGGTCAGT TTTGCTAAAT ACGAGTGGTT TGAGGAYTGG 
AAGGACGACA AAGTGACGAA CCGCGGGCCC GACTACAAAG AGCTGAAACA AATGTTTATC 
GACTCGGCCC TGGAGGTGGT GATGGACGTG TTTCCTAAGA TCACCAGAGA CAAGATTGAG 
TACATTGATG CTGGAACCCC CGTCACAAAC ACGCACTATA TCGCAGCCCC GAAAGGTGAG 
ATCTACGGTG TGGATCATGA CATCGGTCGG TTCAGGCCTG AACTCAACGC TACGATAAGA 
CCTCAGACCC CTCTGAAGAA CCTCTACCTG ACAGGTCAGG ATATGTTTCT GTGTGGTTTC 
GCCGGCGCCC TGGCCGGAGC GCTCACCTGT GGCTCGGTCA TTCTTAACCG CAACCTCCAT 
CTGGACGCCA TCGCCTTGGC CAAAAGAGCA AAACATTTTA ACAAGCAGAA AAAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

retsat ( XM_014022496.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013877950.1 Austrofundulus limnaeus retinol saturase (all-trans-retinol 13,14-reductase) (retsat), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAu02011
Clone ID Related Accession (Same CDS sequence)	XM_014022496.1
Accession Version	XM_014022496.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	all-trans-retinol 13,14-reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952406.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGTCC TTGTGTCCAT TATTTCTGCG GTTCTGATTA TTTTTATGTT AAAATATGTC 
TTCGGCAGCT ACGGACCGAA CCCCTTTGAG AAGGACTGTC GTGAACCGCT GAAAAAGAGA 
GTTTACGACA AGAAAGAGAA GAATAAAGTG CTAAAGCAAG GTTTCCTGGC CAGCAGAGTA 
CCAGAAAACT TGGARGCCAT CGTCATTGGC AGCGGCGTCG GTGGGCTCGG GCTGGCCGTG 
CTGCTGGCTA AAGTCGGAAA GAAAGTTCTG GTTCTGGAGC AGCACAGCAA GGCCGGAGGG 
TGCTGCCACA CGTTCTCCGA GAAGGGCTTC GAGTTTGATG TGGGGATCCA TTACATCGGT 
GACCTCCTGG AGCACAAACC CTTCCGCTGC ATGTTGGACC AGATGACCAA TGGGCAGCTG 
CAGTGGGAGC CTCTGGAGAA CCCGTTTGAC CACGTCCTGC TGGGACCTCC AGAAAACCGC 
CGCAGGTACC CCATCTACAG CGGCAAGACT CGCTTCCCCG AAGAGCTGAA GAAGTGTTTC 
CCCGGAGAGG AGAAAGCCAT CGACGAGTAC CTGCGGCTGG CCAAGAAAAC TAAAAACGGA 
GTTTGGCTCA TGGCTATGCT GAAGCTCCTT CCAGTCTTTC TGACCAAGTT CCTGATCTGC 
ACCGGCCTCG TCGAACGTCT GTCTTTCTTC TTCAAAATGG CACCCCGCAG CCTGACAGAC 
GTGGTCAACG AGCTGACACA GAACCAGGAC CTCAGAGCCG TATTCTCCTA CATTGTTGGC 
ACTTACGGAA AGACTCCTAA AGAGGCCAGC TTTGCCATGC ACAGTCTGCT GGTGACTCAC 
TACCTGGACG GCGCCTGGTA CCCTAAAGGC GGCTCCAGTG AAATCGCATA CCACATGATC 
CCCATTGTGG AGAAGGCGGG AGGCGCTGTT TTGGTCCGAG CCCCGGTGAA CCGGATCCTG 
TTCAATGATG CCAAAGAAGC TTACGGTGTT AGTGTGTTGA AGGGTCAGGA GGAAGTGCAC 
ATCCGCGCCC CCATGATCGT TTCCGATGCC GGCATCTTCA ACACGTACGA GAAGCTGCTG 
CCCGCAGAGC TCCAAGCCCT GCCAGCCATC CAGGAGCAGC TCAGCATGGT GAAACACGGT 
GATGGCGGCT TGAGCATCTT TATGGGTCTG AACGGAACCA AGGAGGAGCT GGGCCTGAAA 
GCGGACAACT ACTGGATCTT TGCTGAGAAC AATTTGGACA AACTGCTGGA GGACTATAAT 
AGTAAAGCGA GAGAACAGGC TGCTCAGAAG ATCCCTCTGC TGTTTGTCGC CTGTCCATCA 
GCTAAAGATC CAACCTGGGA GGAGAGATCA CCGGGAAAGT CAACTTTGAG TCTGGTCAGT 
TTTGCTAAAT ACGAGTGGTT TGAGGAYTGG AAGGACGACA AAGTGACGAA CCGCGGGCCC 
GACTACAAAG AGCTGAAACA AATGTTTATC GACTCGGCCC TGGAGGTGGT GATGGACGTG 
TTTCCTAAGA TCACCAGAGA CAAGATTGAG TACATTGATG CTGGAACCCC CGTCACAAAC 
ACGCACTATA TCGCAGCCCC GAAAGGTGAG ATCTACGGTG TGGATCATGA CATCGGTCGG 
TTCAGGCCTG AACTCAACGC TACGATAAGA CCTCAGACCC CTCTGAAGAA CCTCTACCTG 
ACAGGTCAGG ATATGTTTCT GTGTGGTTTC GCCGGCGCCC TGGCCGGAGC GCTCACCTGT 
GGCTCGGTCA TTCTTAACCG CAACCTCCAT CTGGACGCCA TCGCCTTGGC CAAAAGAGCA 
AAACATTTTA ACAAGCAGAA AAAGGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013877950.1
CDS	55..1884
Translation	MWVLVSIISAVLIIFMLKYVFGSYGPNPFEKDCREPLKKRVYDKKEKNKVLKQGFLASRVPENLnullAIVIGSGVGGLGLAVLLAKVGKKVLVLEQHSKAGGCCHTFSEKGFEFDVGIHYIGDLLEHKPFRCMLDQMTNGQLQWEPLENPFDHVLLGPPENRRRYPIYSGKTRFPEELKKCFPGEEKAIDEYLRLAKKTKNGVWLMAMLKLLPVFLTKFLICTGLVERLSFFFKMAPRSLTDVVNELTQNQDLRAVFSYIVGTYGKTPKEASFAMHSLLVTHYLDGAWYPKGGSSEIAYHMIPIVEKAGGAVLVRAPVNRILFNDAKEAYGVSVLKGQEEVHIRAPMIVSDAGIFNTYEKLLPAELQALPAIQEQLSMVKHGDGGLSIFMGLNGTKEELGLKADNYWIFAENNLDKLLEDYNSKAREQAAQKIPLLFVACPSAKDPTWEERSPGKSTLSLVSFAKYEWFEnullWKDDKVTNRGPDYKELKQMFIDSALEVVMDVFPKITRDKIEYIDAGTPVTNTHYIAAPKGEIYGVDHDIGRFRPELNATIRPQTPLKNLYLTGQDMFLCGFAGALAGALTCGSVILNRNLHLDAIALAKRAKHFNKQKKE

Target ORF information:

RefSeq Version	XM_014022496.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus retinol saturase (all-trans-retinol 13,14-reductase) (retsat), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014022496.1

ORF Insert Sequence:

ATGTGGGTCC TTGTGTCCAT TATTTCTGCG GTTCTGATTA TTTTTATGTT AAAATATGTC 
TTCGGCAGCT ACGGACCGAA CCCCTTTGAG AAGGACTGTC GTGAACCGCT GAAAAAGAGA 
GTTTACGACA AGAAAGAGAA GAATAAAGTG CTAAAGCAAG GTTTCCTGGC CAGCAGAGTA 
CCAGAAAACT TGGARGCCAT CGTCATTGGC AGCGGCGTCG GTGGGCTCGG GCTGGCCGTG 
CTGCTGGCTA AAGTCGGAAA GAAAGTTCTG GTTCTGGAGC AGCACAGCAA GGCCGGAGGG 
TGCTGCCACA CGTTCTCCGA GAAGGGCTTC GAGTTTGATG TGGGGATCCA TTACATCGGT 
GACCTCCTGG AGCACAAACC CTTCCGCTGC ATGTTGGACC AGATGACCAA TGGGCAGCTG 
CAGTGGGAGC CTCTGGAGAA CCCGTTTGAC CACGTCCTGC TGGGACCTCC AGAAAACCGC 
CGCAGGTACC CCATCTACAG CGGCAAGACT CGCTTCCCCG AAGAGCTGAA GAAGTGTTTC 
CCCGGAGAGG AGAAAGCCAT CGACGAGTAC CTGCGGCTGG CCAAGAAAAC TAAAAACGGA 
GTTTGGCTCA TGGCTATGCT GAAGCTCCTT CCAGTCTTTC TGACCAAGTT CCTGATCTGC 
ACCGGCCTCG TCGAACGTCT GTCTTTCTTC TTCAAAATGG CACCCCGCAG CCTGACAGAC 
GTGGTCAACG AGCTGACACA GAACCAGGAC CTCAGAGCCG TATTCTCCTA CATTGTTGGC 
ACTTACGGAA AGACTCCTAA AGAGGCCAGC TTTGCCATGC ACAGTCTGCT GGTGACTCAC 
TACCTGGACG GCGCCTGGTA CCCTAAAGGC GGCTCCAGTG AAATCGCATA CCACATGATC 
CCCATTGTGG AGAAGGCGGG AGGCGCTGTT TTGGTCCGAG CCCCGGTGAA CCGGATCCTG 
TTCAATGATG CCAAAGAAGC TTACGGTGTT AGTGTGTTGA AGGGTCAGGA GGAAGTGCAC 
ATCCGCGCCC CCATGATCGT TTCCGATGCC GGCATCTTCA ACACGTACGA GAAGCTGCTG 
CCCGCAGAGC TCCAAGCCCT GCCAGCCATC CAGGAGCAGC TCAGCATGGT GAAACACGGT 
GATGGCGGCT TGAGCATCTT TATGGGTCTG AACGGAACCA AGGAGGAGCT GGGCCTGAAA 
GCGGACAACT ACTGGATCTT TGCTGAGAAC AATTTGGACA AACTGCTGGA GGACTATAAT 
AGTAAAGCGA GAGAACAGGC TGCTCAGAAG ATCCCTCTGC TGTTTGTCGC CTGTCCATCA 
GCTAAAGATC CAACCTGGGA GGAGAGATCA CCGGGAAAGT CAACTTTGAG TCTGGTCAGT 
TTTGCTAAAT ACGAGTGGTT TGAGGAYTGG AAGGACGACA AAGTGACGAA CCGCGGGCCC 
GACTACAAAG AGCTGAAACA AATGTTTATC GACTCGGCCC TGGAGGTGGT GATGGACGTG 
TTTCCTAAGA TCACCAGAGA CAAGATTGAG TACATTGATG CTGGAACCCC CGTCACAAAC 
ACGCACTATA TCGCAGCCCC GAAAGGTGAG ATCTACGGTG TGGATCATGA CATCGGTCGG 
TTCAGGCCTG AACTCAACGC TACGATAAGA CCTCAGACCC CTCTGAAGAA CCTCTACCTG 
ACAGGTCAGG ATATGTTTCT GTGTGGTTTC GCCGGCGCCC TGGCCGGAGC GCTCACCTGT 
GGCTCGGTCA TTCTTAACCG CAACCTCCAT CTGGACGCCA TCGCCTTGGC CAAAAGAGCA 
AAACATTTTA ACAAGCAGAA AAAGGAGTAG