nuf2 cDNA ORF clone, Austrofundulus limnaeus

Gene
Clones
gRNAs

Summary

Gene Symbol	nuf2
Entrez Gene ID	106527404
Full Name	NUF2, NDC80 kinetochore complex component
Gene Type	protein-coding
Organism	Austrofundulus limnaeus

mRNA and Protein(s)

mRNA	Protein	Name
XM_014022276.1	XP_013877730.1	kinetochore protein Nuf2

Pathways from BioSystems
Gene Ontology

The following nuf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nuf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAu20099	XM_014022276.1 Latest version!	Austrofundulus limnaeus NUF2, NDC80 kinetochore complex component (nuf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nuf2 ( XM_014022276.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013877730.1 Austrofundulus limnaeus NUF2, NDC80 kinetochore complex component (nuf2), mRNA.

Price & Availability↑

CloneID	OAu20099
Clone ID Related Accession (Same CDS sequence)	XM_014022276.1
Accession Version	XM_014022276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Austrofundulus limnaeus
Product	kinetochore protein Nuf2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952724.1) and transcript sequence (GDFR01033897.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 98 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGCTGATA ATACTTTTCC TGTTCACAAC GCGGAGACGT TAGTGAACTT TTATAGAAAT 
GATGTTCTCA CCGGACAAGA AGCAAAACAA CTCACCAAAA GCGACCTGAC TCCGGTTCCG 
AAGCCACAAG CTGTTCAGAC TCTGTACATG AGAGTGTTAC ATCTCCTGAC CGACTTTAGA 
CCCGAGTGTC ACTCCATGGT TCCTCTGTTG GAGAACATCC AGTATCCAGC CTATCATGAG 
GAAGCTACTT ACATTCTGAG TGTCTACACG TGCATGCGTC AGTTCCTGCC CATGTGCATG 
GTGTATGACT TCTCACTCAG TGATCTACTG ACTCCAAAGA AACAGAGGAC TCTGAGCATT 
CTGAGTGCCA TCATGAACTT CCTTCACTTC AGGAAGCAGA GGATGGAAAT GGTTCTGGAC 
AAGCAGGCCA AGTTTAGGTC AGACGTGGAC AGACTGCAGA CGTTGACCAG AAGCATCCAA 
GAAGCAGAGA AGAAGATTGA GATGTTGACA ACCATCCCAC CGGAGCAGCA GGCCGAGGCA 
GATGAGCTGG CAGCTGCTTT CTCTGAGCTG AAGACTAAAA CCATGCACGA GTATCAGGAG 
GCGAGCGCCG TGGACGAGAG TGTTGCAGAG TGGAAGGCCA GAATCGCAGA GACCACACAG 
AAGCTGGCCC AGATAAAGGT GGACGTCGGG GACCTGAAGG AGGACATCAG TACTCTAAAG 
TCTCAGATTG TTGAGTCTCC AGAGGAACTG AAGAGTGAAA TGGAGAGGAT GAGGGAGTGC 
GTTAAAACCA TGAAGATCTC CATCAAAGAC ACAGATGAGC GTCTGGTGGA GCTGCAGAAC 
ATGGTGCAGG GTGTGGGTCA CACCGAGGCT GAGGTCCAGC AGATGTTCAG CCTCCTGCAG 
GACCTGGAGA GCAGCATGAA CTCCACCAAG CAGCTGCAGG AGGAGAAGCA GGAGCTGGTT 
GCTCAGTATG AGAAGAAGCA GAAGGAGTTA AAACATCTGT GTGTGGAGGA GGGTCAGCTG 
AAGAGAGCTC AGGCCATGAA GATGGACAAG GAGTGCAAAC ACAACATTCA AAAACAGAAG 
AAGAAGGAGA TGAAGGAGCA GCACGTTCAG GAAGTGTTGG GGACCTTTGA CCACATCCTC 
CAGAAGCGTG AGGAGATGGC CAACAAAATC CAGGAGATTT CTGCAGAGAA GCAGCAGCTG 
AAGACCAAGA TCAGCAGCCT GAGAGACGTG TGCAGCAAAG AGACAGAGAA GGCTCAGCTG 
CTGTTCGAGA CCCTCTCTAC ATCCATGGAA GAGCTGCACA AGAGGATTGA GATGCAGCTC 
ACAGAACTGA ACCTAAACCT AGAGAGGATG TCCAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013877730.1
CDS	92..1450
Translation	MADNTFPVHNAETLVNFYRNDVLTGQEAKQLTKSDLTPVPKPQAVQTLYMRVLHLLTDFRPECHSMVPLLENIQYPAYHEEATYILSVYTCMRQFLPMCMVYDFSLSDLLTPKKQRTLSILSAIMNFLHFRKQRMEMVLDKQAKFRSDVDRLQTLTRSIQEAEKKIEMLTTIPPEQQAEADELAAAFSELKTKTMHEYQEASAVDESVAEWKARIAETTQKLAQIKVDVGDLKEDISTLKSQIVESPEELKSEMERMRECVKTMKISIKDTDERLVELQNMVQGVGHTEAEVQQMFSLLQDLESSMNSTKQLQEEKQELVAQYEKKQKELKHLCVEEGQLKRAQAMKMDKECKHNIQKQKKKEMKEQHVQEVLGTFDHILQKREEMANKIQEISAEKQQLKTKISSLRDVCSKETEKAQLLFETLSTSMEELHKRIEMQLTELNLNLERMSK

Target ORF information:

RefSeq Version	XM_014022276.1
Organism	Austrofundulus limnaeus
Definition	Austrofundulus limnaeus NUF2, NDC80 kinetochore complex component (nuf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014022276.1

ORF Insert Sequence:

ATGGCTGATA ATACTTTTCC TGTTCACAAC GCGGAGACGT TAGTGAACTT TTATAGAAAT 
GATGTTCTCA CCGGACAAGA AGCAAAACAA CTCACCAAAA GCGACCTGAC TCCGGTTCCG 
AAGCCACAAG CTGTTCAGAC TCTGTACATG AGAGTGTTAC ATCTCCTGAC CGACTTTAGA 
CCCGAGTGTC ACTCCATGGT TCCTCTGTTG GAGAACATCC AGTATCCAGC CTATCATGAG 
GAAGCTACTT ACATTCTGAG TGTCTACACG TGCATGCGTC AGTTCCTGCC CATGTGCATG 
GTGTATGACT TCTCACTCAG TGATCTACTG ACTCCAAAGA AACAGAGGAC TCTGAGCATT 
CTGAGTGCCA TCATGAACTT CCTTCACTTC AGGAAGCAGA GGATGGAAAT GGTTCTGGAC 
AAGCAGGCCA AGTTTAGGTC AGACGTGGAC AGACTGCAGA CGTTGACCAG AAGCATCCAA 
GAAGCAGAGA AGAAGATTGA GATGTTGACA ACCATCCCAC CGGAGCAGCA GGCCGAGGCA 
GATGAGCTGG CAGCTGCTTT CTCTGAGCTG AAGACTAAAA CCATGCACGA GTATCAGGAG 
GCGAGCGCCG TGGACGAGAG TGTTGCAGAG TGGAAGGCCA GAATCGCAGA GACCACACAG 
AAGCTGGCCC AGATAAAGGT GGACGTCGGG GACCTGAAGG AGGACATCAG TACTCTAAAG 
TCTCAGATTG TTGAGTCTCC AGAGGAACTG AAGAGTGAAA TGGAGAGGAT GAGGGAGTGC 
GTTAAAACCA TGAAGATCTC CATCAAAGAC ACAGATGAGC GTCTGGTGGA GCTGCAGAAC 
ATGGTGCAGG GTGTGGGTCA CACCGAGGCT GAGGTCCAGC AGATGTTCAG CCTCCTGCAG 
GACCTGGAGA GCAGCATGAA CTCCACCAAG CAGCTGCAGG AGGAGAAGCA GGAGCTGGTT 
GCTCAGTATG AGAAGAAGCA GAAGGAGTTA AAACATCTGT GTGTGGAGGA GGGTCAGCTG 
AAGAGAGCTC AGGCCATGAA GATGGACAAG GAGTGCAAAC ACAACATTCA AAAACAGAAG 
AAGAAGGAGA TGAAGGAGCA GCACGTTCAG GAAGTGTTGG GGACCTTTGA CCACATCCTC 
CAGAAGCGTG AGGAGATGGC CAACAAAATC CAGGAGATTT CTGCAGAGAA GCAGCAGCTG 
AAGACCAAGA TCAGCAGCCT GAGAGACGTG TGCAGCAAAG AGACAGAGAA GGCTCAGCTG 
CTGTTCGAGA CCCTCTCTAC ATCCATGGAA GAGCTGCACA AGAGGATTGA GATGCAGCTC 
ACAGAACTGA ACCTAAACCT AGAGAGGATG TCCAAGTGA