ORF » Species Summary » Austrofundulus limnaeus » LOC106517999 cDNA ORF clone

LOC106517999 cDNA ORF clone, Austrofundulus limnaeus

The following LOC106517999 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC106517999 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OAu09583 XM_014009062.1
Latest version!		 Austrofundulus limnaeus probable G-protein coupled receptor 22 (LOC106517999), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OAu09583 XM_014009063.1
Latest version!		 Austrofundulus limnaeus probable G-protein coupled receptor 22 (LOC106517999), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OAu09583 XM_014009064.1
Latest version!		 Austrofundulus limnaeus probable G-protein coupled receptor 22 (LOC106517999), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    LOC106517999 ( XM_014009062.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013864516.1 Austrofundulus limnaeus probable G-protein coupled receptor 22 (LOC106517999), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OAu09583
    Clone ID Related Accession (Same CDS sequence) XM_014009062.1 , XM_014009063.1 , XM_014009064.1
    Accession Version XM_014009062.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Austrofundulus limnaeus
    Product probable G-protein coupled receptor 22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952457.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGACCG AAGGCTATCG TGACCTCCTG GAGACCAGCG ATGGTCAGGG GGTAGGCCTG 
    CTGGATGGAG GAGGGGAAGT GGGCATGGAG GAAGGCTGGA GCGCACCATA CCCTCTTGGT 
    TTTCAGGTGT CTTTAACCAC TGTGCTGATA CTGGAGCTTG TGTTAGGCTT CAGCAGCAAC 
    CTGACCGTGC TCGTGCTCTA CTGTGCTCAG TCGAACCTGG TGGATTCCGT CAGCAACCTC 
    GTCACGGTCA ACCTCCATGT GCTCGACATA CTGGTGTGTG TGCTCTGTTT GCCTCTAACA 
    GTCGCTGTGA TCCTGCTACC AGCAAACGAA AATGGAGTCA GCAGCCTGGC CACACTTTGT 
    TGCTTTCACG AAGCCTGTGT CACGTTCACG AGCGTGGCCA CGGCAGTCAA CGTGTTAGTG 
    ATCAGTTTGG ACCGTTACGA CATCTCTGTG CGCCCGGCCA GTCGTCTCCT CACCCCCAGG 
    CGGGCCGCAC TGCTCCTGGC AGCAGTGTGG GCCGTGTCTC TGGCTGTTTT CTTCCTGCCT 
    TTTCTCGAAG GGGACTTCTT CTCCACCACA GCTGAGGATA GTGAGGACGA CAAATCAGAG 
    AGGCAAAGCA ATGAGTCAGA ACTCTCTACT GAACACACGC CTATTTTTTC ATCCATCTCT 
    CCTTTGTTCC CCTCCACTCT GCCCTCCTAC CCTTCACACC ACCCGCCACC TGTATGGCAG 
    AACAGGACTC TGTTGTGTGT TGGAGGGCAG GGTTATTACA CAGGCATGGC TATATATTAC 
    CACTTGTTAC TGCAAGTGCC ATGCTTCTTC ATAGCTGTGG TAGTCATGCT GTTCACTTAC 
    TCTAGGATCT TACAAGCCCT CAACATCCGC ATTGGCTCCC ACATGATGAG GAGTACACGT 
    TCAAAGGACA AAACCTGCAG GATACGCTGC AGGAGGCGAA CAAAGAAGGA CCTGAACCTG 
    CCCACTGAAA CGGTGTCCTC CAATCAGAAC CAGAACCTCA GTCACCCGCC CCTTATCCCT 
    TCTGCCACCC CTACRCCAAC ATCGCCCCCG CCGCTCTCAT CCATGCCTCA AGGGTTGTCC 
    GACAGTGGTG CTACAGTAAC CACTGTCAGC ACTGCTGCCA CCACCCCCAT TGCGACCACA 
    CCAGCCACAC CAGTGTCGCC AACTCCCCCT ACAGCATCAG CCCAGACCCA CGCCTCCACT 
    CATCTGCCTG CCTCATCCAT GGGTGTGCAG GCCTCGGTGT CTGCCATCAT CGCTTTGCGG 
    CGGGCGGTGC GCAGACACAG GGACCGGCGG GAGCGTCAAC GTCGTGTCTT GAAAATGTCC 
    CTCATCATCA TATCCACCTT CCTGGGCTGC TGGGCTCCTC TGTCCGCAGT CAATGTTCTT 
    ATCCTTTGTA TGGGTCCCAG TGACAGCCTG GTGGAGCTTC GGCTGTGCTT CCTGGCCATG 
    GCGTATGGAA CTACCATCTT TCACCCGTTG CTCTATGCGT TCACTAGACA GAAGCTACGG 
    CGCGCCCTGA AAACACGTGT CAAGAAAAGG GTAGTGTCCC TGCTGCAGGT GGACCCAGCT 
    CCCAGTGGAG GGACCGTGAT TCATAACTCT TGGGTGGACG GGGGAGGCCA GAGAAAGGGT 
    CGCAAGTCAA GAATGGAGGC CAGCGACGGC ACTGATCGAT GTCTCACAGA AGCAGTGAGG 
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013864516.1
    CDS354..2039
    Translation

    Target ORF information:

    RefSeq Version XM_014009062.1
    Organism Austrofundulus limnaeus
    Definition Austrofundulus limnaeus probable G-protein coupled receptor 22 (LOC106517999), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014009062.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGACCG AAGGCTATCG TGACCTCCTG GAGACCAGCG ATGGTCAGGG GGTAGGCCTG 
    CTGGATGGAG GAGGGGAAGT GGGCATGGAG GAAGGCTGGA GCGCACCATA CCCTCTTGGT 
    TTTCAGGTGT CTTTAACCAC TGTGCTGATA CTGGAGCTTG TGTTAGGCTT CAGCAGCAAC 
    CTGACCGTGC TCGTGCTCTA CTGTGCTCAG TCGAACCTGG TGGATTCCGT CAGCAACCTC 
    GTCACGGTCA ACCTCCATGT GCTCGACATA CTGGTGTGTG TGCTCTGTTT GCCTCTAACA 
    GTCGCTGTGA TCCTGCTACC AGCAAACGAA AATGGAGTCA GCAGCCTGGC CACACTTTGT 
    TGCTTTCACG AAGCCTGTGT CACGTTCACG AGCGTGGCCA CGGCAGTCAA CGTGTTAGTG 
    ATCAGTTTGG ACCGTTACGA CATCTCTGTG CGCCCGGCCA GTCGTCTCCT CACCCCCAGG 
    CGGGCCGCAC TGCTCCTGGC AGCAGTGTGG GCCGTGTCTC TGGCTGTTTT CTTCCTGCCT 
    TTTCTCGAAG GGGACTTCTT CTCCACCACA GCTGAGGATA GTGAGGACGA CAAATCAGAG 
    AGGCAAAGCA ATGAGTCAGA ACTCTCTACT GAACACACGC CTATTTTTTC ATCCATCTCT 
    CCTTTGTTCC CCTCCACTCT GCCCTCCTAC CCTTCACACC ACCCGCCACC TGTATGGCAG 
    AACAGGACTC TGTTGTGTGT TGGAGGGCAG GGTTATTACA CAGGCATGGC TATATATTAC 
    CACTTGTTAC TGCAAGTGCC ATGCTTCTTC ATAGCTGTGG TAGTCATGCT GTTCACTTAC 
    TCTAGGATCT TACAAGCCCT CAACATCCGC ATTGGCTCCC ACATGATGAG GAGTACACGT 
    TCAAAGGACA AAACCTGCAG GATACGCTGC AGGAGGCGAA CAAAGAAGGA CCTGAACCTG 
    CCCACTGAAA CGGTGTCCTC CAATCAGAAC CAGAACCTCA GTCACCCGCC CCTTATCCCT 
    TCTGCCACCC CTACRCCAAC ATCGCCCCCG CCGCTCTCAT CCATGCCTCA AGGGTTGTCC 
    GACAGTGGTG CTACAGTAAC CACTGTCAGC ACTGCTGCCA CCACCCCCAT TGCGACCACA 
    CCAGCCACAC CAGTGTCGCC AACTCCCCCT ACAGCATCAG CCCAGACCCA CGCCTCCACT 
    CATCTGCCTG CCTCATCCAT GGGTGTGCAG GCCTCGGTGT CTGCCATCAT CGCTTTGCGG 
    CGGGCGGTGC GCAGACACAG GGACCGGCGG GAGCGTCAAC GTCGTGTCTT GAAAATGTCC 
    CTCATCATCA TATCCACCTT CCTGGGCTGC TGGGCTCCTC TGTCCGCAGT CAATGTTCTT 
    ATCCTTTGTA TGGGTCCCAG TGACAGCCTG GTGGAGCTTC GGCTGTGCTT CCTGGCCATG 
    GCGTATGGAA CTACCATCTT TCACCCGTTG CTCTATGCGT TCACTAGACA GAAGCTACGG 
    CGCGCCCTGA AAACACGTGT CAAGAAAAGG GTAGTGTCCC TGCTGCAGGT GGACCCAGCT 
    CCCAGTGGAG GGACCGTGAT TCATAACTCT TGGGTGGACG GGGGAGGCCA GAGAAAGGGT 
    CGCAAGTCAA GAATGGAGGC CAGCGACGGC ACTGATCGAT GTCTCACAGA AGCAGTGAGG 
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC106517999 ( XM_014009063.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013864517.1 Austrofundulus limnaeus probable G-protein coupled receptor 22 (LOC106517999), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OAu09583
    Clone ID Related Accession (Same CDS sequence) XM_014009062.1 , XM_014009063.1 , XM_014009064.1
    Accession Version XM_014009063.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Austrofundulus limnaeus
    Product probable G-protein coupled receptor 22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952457.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGACCG AAGGCTATCG TGACCTCCTG GAGACCAGCG ATGGTCAGGG GGTAGGCCTG 
    CTGGATGGAG GAGGGGAAGT GGGCATGGAG GAAGGCTGGA GCGCACCATA CCCTCTTGGT 
    TTTCAGGTGT CTTTAACCAC TGTGCTGATA CTGGAGCTTG TGTTAGGCTT CAGCAGCAAC 
    CTGACCGTGC TCGTGCTCTA CTGTGCTCAG TCGAACCTGG TGGATTCCGT CAGCAACCTC 
    GTCACGGTCA ACCTCCATGT GCTCGACATA CTGGTGTGTG TGCTCTGTTT GCCTCTAACA 
    GTCGCTGTGA TCCTGCTACC AGCAAACGAA AATGGAGTCA GCAGCCTGGC CACACTTTGT 
    TGCTTTCACG AAGCCTGTGT CACGTTCACG AGCGTGGCCA CGGCAGTCAA CGTGTTAGTG 
    ATCAGTTTGG ACCGTTACGA CATCTCTGTG CGCCCGGCCA GTCGTCTCCT CACCCCCAGG 
    CGGGCCGCAC TGCTCCTGGC AGCAGTGTGG GCCGTGTCTC TGGCTGTTTT CTTCCTGCCT 
    TTTCTCGAAG GGGACTTCTT CTCCACCACA GCTGAGGATA GTGAGGACGA CAAATCAGAG 
    AGGCAAAGCA ATGAGTCAGA ACTCTCTACT GAACACACGC CTATTTTTTC ATCCATCTCT 
    CCTTTGTTCC CCTCCACTCT GCCCTCCTAC CCTTCACACC ACCCGCCACC TGTATGGCAG 
    AACAGGACTC TGTTGTGTGT TGGAGGGCAG GGTTATTACA CAGGCATGGC TATATATTAC 
    CACTTGTTAC TGCAAGTGCC ATGCTTCTTC ATAGCTGTGG TAGTCATGCT GTTCACTTAC 
    TCTAGGATCT TACAAGCCCT CAACATCCGC ATTGGCTCCC ACATGATGAG GAGTACACGT 
    TCAAAGGACA AAACCTGCAG GATACGCTGC AGGAGGCGAA CAAAGAAGGA CCTGAACCTG 
    CCCACTGAAA CGGTGTCCTC CAATCAGAAC CAGAACCTCA GTCACCCGCC CCTTATCCCT 
    TCTGCCACCC CTACRCCAAC ATCGCCCCCG CCGCTCTCAT CCATGCCTCA AGGGTTGTCC 
    GACAGTGGTG CTACAGTAAC CACTGTCAGC ACTGCTGCCA CCACCCCCAT TGCGACCACA 
    CCAGCCACAC CAGTGTCGCC AACTCCCCCT ACAGCATCAG CCCAGACCCA CGCCTCCACT 
    CATCTGCCTG CCTCATCCAT GGGTGTGCAG GCCTCGGTGT CTGCCATCAT CGCTTTGCGG 
    CGGGCGGTGC GCAGACACAG GGACCGGCGG GAGCGTCAAC GTCGTGTCTT GAAAATGTCC 
    CTCATCATCA TATCCACCTT CCTGGGCTGC TGGGCTCCTC TGTCCGCAGT CAATGTTCTT 
    ATCCTTTGTA TGGGTCCCAG TGACAGCCTG GTGGAGCTTC GGCTGTGCTT CCTGGCCATG 
    GCGTATGGAA CTACCATCTT TCACCCGTTG CTCTATGCGT TCACTAGACA GAAGCTACGG 
    CGCGCCCTGA AAACACGTGT CAAGAAAAGG GTAGTGTCCC TGCTGCAGGT GGACCCAGCT 
    CCCAGTGGAG GGACCGTGAT TCATAACTCT TGGGTGGACG GGGGAGGCCA GAGAAAGGGT 
    CGCAAGTCAA GAATGGAGGC CAGCGACGGC ACTGATCGAT GTCTCACAGA AGCAGTGAGG 
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013864517.1
    CDS250..1935
    Translation

    Target ORF information:

    RefSeq Version XM_014009063.1
    Organism Austrofundulus limnaeus
    Definition Austrofundulus limnaeus probable G-protein coupled receptor 22 (LOC106517999), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014009063.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGACCG AAGGCTATCG TGACCTCCTG GAGACCAGCG ATGGTCAGGG GGTAGGCCTG 
    CTGGATGGAG GAGGGGAAGT GGGCATGGAG GAAGGCTGGA GCGCACCATA CCCTCTTGGT 
    TTTCAGGTGT CTTTAACCAC TGTGCTGATA CTGGAGCTTG TGTTAGGCTT CAGCAGCAAC 
    CTGACCGTGC TCGTGCTCTA CTGTGCTCAG TCGAACCTGG TGGATTCCGT CAGCAACCTC 
    GTCACGGTCA ACCTCCATGT GCTCGACATA CTGGTGTGTG TGCTCTGTTT GCCTCTAACA 
    GTCGCTGTGA TCCTGCTACC AGCAAACGAA AATGGAGTCA GCAGCCTGGC CACACTTTGT 
    TGCTTTCACG AAGCCTGTGT CACGTTCACG AGCGTGGCCA CGGCAGTCAA CGTGTTAGTG 
    ATCAGTTTGG ACCGTTACGA CATCTCTGTG CGCCCGGCCA GTCGTCTCCT CACCCCCAGG 
    CGGGCCGCAC TGCTCCTGGC AGCAGTGTGG GCCGTGTCTC TGGCTGTTTT CTTCCTGCCT 
    TTTCTCGAAG GGGACTTCTT CTCCACCACA GCTGAGGATA GTGAGGACGA CAAATCAGAG 
    AGGCAAAGCA ATGAGTCAGA ACTCTCTACT GAACACACGC CTATTTTTTC ATCCATCTCT 
    CCTTTGTTCC CCTCCACTCT GCCCTCCTAC CCTTCACACC ACCCGCCACC TGTATGGCAG 
    AACAGGACTC TGTTGTGTGT TGGAGGGCAG GGTTATTACA CAGGCATGGC TATATATTAC 
    CACTTGTTAC TGCAAGTGCC ATGCTTCTTC ATAGCTGTGG TAGTCATGCT GTTCACTTAC 
    TCTAGGATCT TACAAGCCCT CAACATCCGC ATTGGCTCCC ACATGATGAG GAGTACACGT 
    TCAAAGGACA AAACCTGCAG GATACGCTGC AGGAGGCGAA CAAAGAAGGA CCTGAACCTG 
    CCCACTGAAA CGGTGTCCTC CAATCAGAAC CAGAACCTCA GTCACCCGCC CCTTATCCCT 
    TCTGCCACCC CTACRCCAAC ATCGCCCCCG CCGCTCTCAT CCATGCCTCA AGGGTTGTCC 
    GACAGTGGTG CTACAGTAAC CACTGTCAGC ACTGCTGCCA CCACCCCCAT TGCGACCACA 
    CCAGCCACAC CAGTGTCGCC AACTCCCCCT ACAGCATCAG CCCAGACCCA CGCCTCCACT 
    CATCTGCCTG CCTCATCCAT GGGTGTGCAG GCCTCGGTGT CTGCCATCAT CGCTTTGCGG 
    CGGGCGGTGC GCAGACACAG GGACCGGCGG GAGCGTCAAC GTCGTGTCTT GAAAATGTCC 
    CTCATCATCA TATCCACCTT CCTGGGCTGC TGGGCTCCTC TGTCCGCAGT CAATGTTCTT 
    ATCCTTTGTA TGGGTCCCAG TGACAGCCTG GTGGAGCTTC GGCTGTGCTT CCTGGCCATG 
    GCGTATGGAA CTACCATCTT TCACCCGTTG CTCTATGCGT TCACTAGACA GAAGCTACGG 
    CGCGCCCTGA AAACACGTGT CAAGAAAAGG GTAGTGTCCC TGCTGCAGGT GGACCCAGCT 
    CCCAGTGGAG GGACCGTGAT TCATAACTCT TGGGTGGACG GGGGAGGCCA GAGAAAGGGT 
    CGCAAGTCAA GAATGGAGGC CAGCGACGGC ACTGATCGAT GTCTCACAGA AGCAGTGAGG 
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    LOC106517999 ( XM_014009064.1 ) cDNA ORF clone, Austrofundulus limnaeus -> XP_013864518.1 Austrofundulus limnaeus probable G-protein coupled receptor 22 (LOC106517999), transcript variant X3, mRNA.
    Price & Availability↑
    CloneID OAu09583
    Clone ID Related Accession (Same CDS sequence) XM_014009062.1 , XM_014009063.1 , XM_014009064.1
    Accession Version XM_014009064.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Austrofundulus limnaeus
    Product probable G-protein coupled receptor 22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_013952457.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Austrofundulus limnaeus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGACCG AAGGCTATCG TGACCTCCTG GAGACCAGCG ATGGTCAGGG GGTAGGCCTG 
    CTGGATGGAG GAGGGGAAGT GGGCATGGAG GAAGGCTGGA GCGCACCATA CCCTCTTGGT 
    TTTCAGGTGT CTTTAACCAC TGTGCTGATA CTGGAGCTTG TGTTAGGCTT CAGCAGCAAC 
    CTGACCGTGC TCGTGCTCTA CTGTGCTCAG TCGAACCTGG TGGATTCCGT CAGCAACCTC 
    GTCACGGTCA ACCTCCATGT GCTCGACATA CTGGTGTGTG TGCTCTGTTT GCCTCTAACA 
    GTCGCTGTGA TCCTGCTACC AGCAAACGAA AATGGAGTCA GCAGCCTGGC CACACTTTGT 
    TGCTTTCACG AAGCCTGTGT CACGTTCACG AGCGTGGCCA CGGCAGTCAA CGTGTTAGTG 
    ATCAGTTTGG ACCGTTACGA CATCTCTGTG CGCCCGGCCA GTCGTCTCCT CACCCCCAGG 
    CGGGCCGCAC TGCTCCTGGC AGCAGTGTGG GCCGTGTCTC TGGCTGTTTT CTTCCTGCCT 
    TTTCTCGAAG GGGACTTCTT CTCCACCACA GCTGAGGATA GTGAGGACGA CAAATCAGAG 
    AGGCAAAGCA ATGAGTCAGA ACTCTCTACT GAACACACGC CTATTTTTTC ATCCATCTCT 
    CCTTTGTTCC CCTCCACTCT GCCCTCCTAC CCTTCACACC ACCCGCCACC TGTATGGCAG 
    AACAGGACTC TGTTGTGTGT TGGAGGGCAG GGTTATTACA CAGGCATGGC TATATATTAC 
    CACTTGTTAC TGCAAGTGCC ATGCTTCTTC ATAGCTGTGG TAGTCATGCT GTTCACTTAC 
    TCTAGGATCT TACAAGCCCT CAACATCCGC ATTGGCTCCC ACATGATGAG GAGTACACGT 
    TCAAAGGACA AAACCTGCAG GATACGCTGC AGGAGGCGAA CAAAGAAGGA CCTGAACCTG 
    CCCACTGAAA CGGTGTCCTC CAATCAGAAC CAGAACCTCA GTCACCCGCC CCTTATCCCT 
    TCTGCCACCC CTACRCCAAC ATCGCCCCCG CCGCTCTCAT CCATGCCTCA AGGGTTGTCC 
    GACAGTGGTG CTACAGTAAC CACTGTCAGC ACTGCTGCCA CCACCCCCAT TGCGACCACA 
    CCAGCCACAC CAGTGTCGCC AACTCCCCCT ACAGCATCAG CCCAGACCCA CGCCTCCACT 
    CATCTGCCTG CCTCATCCAT GGGTGTGCAG GCCTCGGTGT CTGCCATCAT CGCTTTGCGG 
    CGGGCGGTGC GCAGACACAG GGACCGGCGG GAGCGTCAAC GTCGTGTCTT GAAAATGTCC 
    CTCATCATCA TATCCACCTT CCTGGGCTGC TGGGCTCCTC TGTCCGCAGT CAATGTTCTT 
    ATCCTTTGTA TGGGTCCCAG TGACAGCCTG GTGGAGCTTC GGCTGTGCTT CCTGGCCATG 
    GCGTATGGAA CTACCATCTT TCACCCGTTG CTCTATGCGT TCACTAGACA GAAGCTACGG 
    CGCGCCCTGA AAACACGTGT CAAGAAAAGG GTAGTGTCCC TGCTGCAGGT GGACCCAGCT 
    CCCAGTGGAG GGACCGTGAT TCATAACTCT TGGGTGGACG GGGGAGGCCA GAGAAAGGGT 
    CGCAAGTCAA GAATGGAGGC CAGCGACGGC ACTGATCGAT GTCTCACAGA AGCAGTGAGG 
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013864518.1
    CDS183..1868
    Translation

    Target ORF information:

    RefSeq Version XM_014009064.1
    Organism Austrofundulus limnaeus
    Definition Austrofundulus limnaeus probable G-protein coupled receptor 22 (LOC106517999), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_014009064.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGACCG AAGGCTATCG TGACCTCCTG GAGACCAGCG ATGGTCAGGG GGTAGGCCTG 
    CTGGATGGAG GAGGGGAAGT GGGCATGGAG GAAGGCTGGA GCGCACCATA CCCTCTTGGT 
    TTTCAGGTGT CTTTAACCAC TGTGCTGATA CTGGAGCTTG TGTTAGGCTT CAGCAGCAAC 
    CTGACCGTGC TCGTGCTCTA CTGTGCTCAG TCGAACCTGG TGGATTCCGT CAGCAACCTC 
    GTCACGGTCA ACCTCCATGT GCTCGACATA CTGGTGTGTG TGCTCTGTTT GCCTCTAACA 
    GTCGCTGTGA TCCTGCTACC AGCAAACGAA AATGGAGTCA GCAGCCTGGC CACACTTTGT 
    TGCTTTCACG AAGCCTGTGT CACGTTCACG AGCGTGGCCA CGGCAGTCAA CGTGTTAGTG 
    ATCAGTTTGG ACCGTTACGA CATCTCTGTG CGCCCGGCCA GTCGTCTCCT CACCCCCAGG 
    CGGGCCGCAC TGCTCCTGGC AGCAGTGTGG GCCGTGTCTC TGGCTGTTTT CTTCCTGCCT 
    TTTCTCGAAG GGGACTTCTT CTCCACCACA GCTGAGGATA GTGAGGACGA CAAATCAGAG 
    AGGCAAAGCA ATGAGTCAGA ACTCTCTACT GAACACACGC CTATTTTTTC ATCCATCTCT 
    CCTTTGTTCC CCTCCACTCT GCCCTCCTAC CCTTCACACC ACCCGCCACC TGTATGGCAG 
    AACAGGACTC TGTTGTGTGT TGGAGGGCAG GGTTATTACA CAGGCATGGC TATATATTAC 
    CACTTGTTAC TGCAAGTGCC ATGCTTCTTC ATAGCTGTGG TAGTCATGCT GTTCACTTAC 
    TCTAGGATCT TACAAGCCCT CAACATCCGC ATTGGCTCCC ACATGATGAG GAGTACACGT 
    TCAAAGGACA AAACCTGCAG GATACGCTGC AGGAGGCGAA CAAAGAAGGA CCTGAACCTG 
    CCCACTGAAA CGGTGTCCTC CAATCAGAAC CAGAACCTCA GTCACCCGCC CCTTATCCCT 
    TCTGCCACCC CTACRCCAAC ATCGCCCCCG CCGCTCTCAT CCATGCCTCA AGGGTTGTCC 
    GACAGTGGTG CTACAGTAAC CACTGTCAGC ACTGCTGCCA CCACCCCCAT TGCGACCACA 
    CCAGCCACAC CAGTGTCGCC AACTCCCCCT ACAGCATCAG CCCAGACCCA CGCCTCCACT 
    CATCTGCCTG CCTCATCCAT GGGTGTGCAG GCCTCGGTGT CTGCCATCAT CGCTTTGCGG 
    CGGGCGGTGC GCAGACACAG GGACCGGCGG GAGCGTCAAC GTCGTGTCTT GAAAATGTCC 
    CTCATCATCA TATCCACCTT CCTGGGCTGC TGGGCTCCTC TGTCCGCAGT CAATGTTCTT 
    ATCCTTTGTA TGGGTCCCAG TGACAGCCTG GTGGAGCTTC GGCTGTGCTT CCTGGCCATG 
    GCGTATGGAA CTACCATCTT TCACCCGTTG CTCTATGCGT TCACTAGACA GAAGCTACGG 
    CGCGCCCTGA AAACACGTGT CAAGAAAAGG GTAGTGTCCC TGCTGCAGGT GGACCCAGCT 
    CCCAGTGGAG GGACCGTGAT TCATAACTCT TGGGTGGACG GGGGAGGCCA GAGAAAGGGT 
    CGCAAGTCAA GAATGGAGGC CAGCGACGGC ACTGATCGAT GTCTCACAGA AGCAGTGAGG 
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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