ZNF516 cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNF516
Entrez Gene ID	105406989
Full Name	zinc finger protein 516
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011585941.1	XP_011584243.1	zinc finger protein 516 isoform X1
XM_011585942.1	XP_011584244.1	zinc finger protein 516 isoform X2

Pathways from BioSystems
Gene Ontology

The following ZNF516 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF516 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq22803	XM_011585942.1 Latest version!	Aquila chrysaetos canadensis zinc finger protein 516 (ZNF516), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OAq22802	XM_011585941.1 Latest version!	Aquila chrysaetos canadensis zinc finger protein 516 (ZNF516), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNF516 ( XM_011585942.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011584244.1 Aquila chrysaetos canadensis zinc finger protein 516 (ZNF516), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq22803
Clone ID Related Accession (Same CDS sequence)	XM_011585942.1
Accession Version	XM_011585942.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	zinc finger protein 516 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950922.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAA ACGCGGTCCA GCCCTCCTCT GCTGCCGGAC ATGCTTTTGT CCTCCTGCCC 
CGCTCACCCG ATGTTGCAGT AATGCAGATG GATCGTAGTA GAGAGGCAGA AATGGAGTTA 
CGGAGATCCT CCAGCCCAGG CAAGCTAAGC AAGAATGACG TGGATGCTGA CAAGACCATG 
TGCCACAGCT GCTGCATCTG CGGTAAAAGT TTCCCTTTTC AGAGCTCCTT GTCGCAGCAC 
ATGAGGAAGC ACACGGGCGA GAAGCCCTAC AAGTGCCCTT ACTGTGACCA CAGAGCTTCC 
CAAAAGGGCA ACCTGAAGAT CCACATCCGT AGTCACAGAA CAGGCACTTT AAGTCAGGGG 
CATGAGGCGG AGATGGGAGA GGCGCAGCTG GGGGAGATGG GTGTTTCGGA GGGCCTCGGC 
GGGTGCACCA GCCCCACTAA AAGTACGTCC GCCTGCAACA GGATCTTGAA CGGTACGGCT 
CAGGTAGATA GCAGCAAGAT CTTGTTGAGA AGCAGCAAGA AGGAGGTCAT GGAGGCGGCG 
CCAGCCGAGG AGGATGGCAA GCTGACCTCT TACCAGTGCA CCTTCTGCAA AAACAAATTT 
GAAAGGAAGA AGGACTTGGA GCAGCACCTG CACCAGGTCC ACAAACCGTT CAAGTGCAGG 
TTGTGTAGCT ACATGACCCT GAGGGAGGAG ACGCTGTTAA ACCACATAGA GAAGGACCAT 
ATAACAGCTC AGGTCCCGAA TGGGGAGGCC TACACTGAGA ACTGCAAGAG CGAGCTGAGC 
GCGGGCGAGT TTCCATGTGA AGTCTGTGGC CAGGCCTTTA GTCAAACCTG GTTCCTGAAA 
GCTCACATGA AAAAGCACAG GGGGTCATTT GATCACGGGT GCCACATTTG TGGCAGGAGA 
TTCAAAGAGC CCTGGTTTCT CAAAAACCAC ATGAAGTCGC ACGGCCCCAA GACCGGGAGC 
AAAAACAAAC CAAAAAATGA TTTGGAGCTC ATAGCCACTA TAAATGACGT GATACAGGAG 
GAGACGATTG TGACGGGCCT CTCTCTGTAC GAAGTTTGCA CCAAGTGTGG AAATCTGTTT 
ACAAATATGG AAAGCCTGAA AGCGCATAAC GCTGTTCACT ACCGGGCGCA GCGGGGCAGC 
GCCGGGGATA AAGCCGAGGG CTTAATTGAT GGGAGCCTAA GCTCCTCGGT AACAAAGCAG 
TTTTTCTTGC AGTGTCTCAA TCTAAGGCCA TCTGTAGGGC CGGATAGAGT TACAAGAGGA 
CAGCCTGGGA AAAGAGTAGC CGAGCTGGAT CCGGTCAGTA GCTACCAAGC TTGGCAGCTG 
GCCACCAAAG GAAAAGTAGT AGAGCCCTCA GAGTATGTGA AGTATGTGGG ATGGGACGAG 
GCCCTGGCGG ATGCGGACGT GACTTACGAC AAGGATAAGA GAGAGTATAT CCTTGTCAAC 
CAGGAGAAGC GCAAGCGAGA CCAGGACTCG CCCAGCTCTT CCAGCAACCC CAAGAAGAAG 
AGCTGCACCG GCGGGCGCCC GGAGAAAACC GGCGGTGCCC CGCCGGGGGA GAGCTGCCCG 
CTCGCCCAGG GGGACCTGGA CTACCGCCCT GCCTCACGGC AGAGCCGGCG GGCCGCCCAG 
AACAAGTCCA CCGAGTGCTT TGAGTGCGGG AAGATCTTCC GCACCTACCA CCAAATGGTG 
CTGCACTCCC GGGTGCACCG CAAGGAGCGG AGGAGCTGCG GCGAGGGCGG GACGGCTGCC 
CAGCCCGACC GCTACGGCTC TAGCAGCGAG GAAGGGGACT CGGGGTCCGT CAGCGGGCCC 
AGCACCCCCG GCTCTGCGTC CGCCCAGGAG GACTCGGTCA CCTCCGTCCT GGGGGAGGAG 
GGGGCCGAGG AGAGCTCGGA GGAGGGAGCA GCCAACCCCT CGCTAGATGA AAAGCCATAC 
CACTGCAACT TTTCTGAAGA GGAAACCCCG ATGGTAACAT CTCAGTTGGA TGAACTCCCG 
AACCTGGGAA GCTGCAATGC CAGAGAAAAT GAGGCCAGGG AATCTAAACC AGAGATGCCT 
ACCCTGGTGT CAGCACTGGA GAGTGTCATC AAGGAGCCCT TTTCGAAATA CCAAGATCCA 
AAAGGTTCTG CAGACATAAA GATGCCAGCA TTTCACCACA GCCAAGGGCT TCCTTGCTCC 
AGTCGCATTG CTAGCTTTGC TTCGGGTGTG GGCCAGATAT CTTCAAACAT AGTCATGGAC 
TTGAAAACAT CACTTGAAGT GCAGGCTAGT CGCTCTAGAG AAAATTTGCT AGACTTGCAC 
AGGGAACATC CTCTGACAGA AAAAGGTGCT GAAGCTCAAG AGGTAGCTCC ACTCGATTTG 
AGTGAAAAAT CAACAAGGGA TAATTCATGC AATAAAAATC TGAATACATC CTTGCAGGCT 
GCTTTAATTG TCTACCCATG TCCTTTCTGC AGTCACAAGA CCTACTACCC TGAAGTCCTG 
TGGATGCACA AAAGAATTTT GCACAAAATC AGCTGTAACT CGATGGTCCC CCCTTGGGTG 
CAACAGAATG GATTCAAGAG TATTAAAAAC AACCTGGTCT TTCTGGCAAG GAGTGGGCGT 
ACCGGCCCCC CTCCTGTTCT CGGCGGTAAG GAATGCCAGC CTTTGCCCAT TGCCAGATTT 
ACACGCACTC AAGTGCCAAG TGCATTGCCA GGATCCAAAA GCAGCTCTCT TTCTGTTGGG 
ATGGCTGCAA AGTCCGGGAG TACGCATCAG TCAAAGGACA GTCATGCCTT CGGCCACTGT 
GGTCCACGCT TATCAGGTCT GGATGGGTAC AGACAGCCCA AGTTAAACCA TTCCCAGGAG 
CAATACGGCA TAGCAGCACA ACAAAAAAGT AAATACGAAG CAAATTCCAA ACTTATGCAA 
ATGGGAGCCT ACAGCAGAAG TGTAACGCCT ACTCAAACGG TCATATCCAG GCCTAGCACA 
CAGCCCACCA ACAGTAAGCA AGTGGAAAAG TACGTGGTTC CCCAAGGAAG TACTAGTTTT 
GCCCCTCCAG GTAAGCACTG TGCATCCGAC TCCATGAAGG CCAAATTCAA CCCACCGCCC 
CAGTATCATC CTCTGTGTAA AAGCGAGCAG TACACTAAAC ACGAAGAGCC ATCGGTGCCT 
CAGAGGGATT CTCATGTGAA GGCTGGGAAT GAGATGAGGA CATTGGCAAA CTGCACAGCG 
GGAGCACGAG CGAGTCCACT GCTGCAGCCC CAGCCTAGCG CCGCGGGAGT TTCTCCGGCT 
TTACACTCCA GCAAACAGGA TCTGGGATCA GACGGGCATG AGAAACGTTT GGACGTTTTA 
AATATCTTTA AAACATACAT TCCAAAGGAC CTTGCTTCGT TGTACCAAAC CTGCGGTGCC 
AACAGCCCTG TCCTGGATCA CGCAGGGATG CTCAGGACAC AAACACGCCA AGGAGACTGC 
GTCTGCAGAG AATGTGGAAA ATGCTTTACC CAACCCAGTC ACCTCAGGAC TCATCAGAGG 
TCCCATGCAG TGGTATTTGA GTCTAATGGA CTCCGAGGTA CTGAAGTTCA CACCACCTCC 
GCAGATGCCC CAAAACAAGG GAGAGACCAC GGCGGCGCGG AGGTCGCCCA CACGCTGCCT 
CTCCGGAAGG GAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011584244.1
CDS	120..3737
Translation	MIENAVQPSSAAGHAFVLLPRSPDVAVMQMDRSREAEMELRRSSSPGKLSKNDVDADKTMCHSCCICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRASQKGNLKIHIRSHRTGTLSQGHEAEMGEAQLGEMGVSEGLGGCTSPTKSTSACNRILNGTAQVDSSKILLRSSKKEVMEAAPAEEDGKLTSYQCTFCKNKFERKKDLEQHLHQVHKPFKCRLCSYMTLREETLLNHIEKDHITAQVPNGEAYTENCKSELSAGEFPCEVCGQAFSQTWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGSKNKPKNDLELIATINDVIQEETIVTGLSLYEVCTKCGNLFTNMESLKAHNAVHYRAQRGSAGDKAEGLIDGSLSSSVTKQFFLQCLNLRPSVGPDRVTRGQPGKRVAELDPVSSYQAWQLATKGKVVEPSEYVKYVGWDEALADADVTYDKDKREYILVNQEKRKRDQDSPSSSSNPKKKSCTGGRPEKTGGAPPGESCPLAQGDLDYRPASRQSRRAAQNKSTECFECGKIFRTYHQMVLHSRVHRKERRSCGEGGTAAQPDRYGSSSEEGDSGSVSGPSTPGSASAQEDSVTSVLGEEGAEESSEEGAANPSLDEKPYHCNFSEEETPMVTSQLDELPNLGSCNARENEARESKPEMPTLVSALESVIKEPFSKYQDPKGSADIKMPAFHHSQGLPCSSRIASFASGVGQISSNIVMDLKTSLEVQASRSRENLLDLHREHPLTEKGAEAQEVAPLDLSEKSTRDNSCNKNLNTSLQAALIVYPCPFCSHKTYYPEVLWMHKRILHKISCNSMVPPWVQQNGFKSIKNNLVFLARSGRTGPPPVLGGKECQPLPIARFTRTQVPSALPGSKSSSLSVGMAAKSGSTHQSKDSHAFGHCGPRLSGLDGYRQPKLNHSQEQYGIAAQQKSKYEANSKLMQMGAYSRSVTPTQTVISRPSTQPTNSKQVEKYVVPQGSTSFAPPGKHCASDSMKAKFNPPPQYHPLCKSEQYTKHEEPSVPQRDSHVKAGNEMRTLANCTAGARASPLLQPQPSAAGVSPALHSSKQDLGSDGHEKRLDVLNIFKTYIPKDLASLYQTCGANSPVLDHAGMLRTQTRQGDCVCRECGKCFTQPSHLRTHQRSHAVVFESNGLRGTEVHTTSADAPKQGRDHGGAEVAHTLPLRKGT

Target ORF information:

RefSeq Version	XM_011585942.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis zinc finger protein 516 (ZNF516), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011585942.1

ORF Insert Sequence:

ATGATTGAAA ACGCGGTCCA GCCCTCCTCT GCTGCCGGAC ATGCTTTTGT CCTCCTGCCC 
CGCTCACCCG ATGTTGCAGT AATGCAGATG GATCGTAGTA GAGAGGCAGA AATGGAGTTA 
CGGAGATCCT CCAGCCCAGG CAAGCTAAGC AAGAATGACG TGGATGCTGA CAAGACCATG 
TGCCACAGCT GCTGCATCTG CGGTAAAAGT TTCCCTTTTC AGAGCTCCTT GTCGCAGCAC 
ATGAGGAAGC ACACGGGCGA GAAGCCCTAC AAGTGCCCTT ACTGTGACCA CAGAGCTTCC 
CAAAAGGGCA ACCTGAAGAT CCACATCCGT AGTCACAGAA CAGGCACTTT AAGTCAGGGG 
CATGAGGCGG AGATGGGAGA GGCGCAGCTG GGGGAGATGG GTGTTTCGGA GGGCCTCGGC 
GGGTGCACCA GCCCCACTAA AAGTACGTCC GCCTGCAACA GGATCTTGAA CGGTACGGCT 
CAGGTAGATA GCAGCAAGAT CTTGTTGAGA AGCAGCAAGA AGGAGGTCAT GGAGGCGGCG 
CCAGCCGAGG AGGATGGCAA GCTGACCTCT TACCAGTGCA CCTTCTGCAA AAACAAATTT 
GAAAGGAAGA AGGACTTGGA GCAGCACCTG CACCAGGTCC ACAAACCGTT CAAGTGCAGG 
TTGTGTAGCT ACATGACCCT GAGGGAGGAG ACGCTGTTAA ACCACATAGA GAAGGACCAT 
ATAACAGCTC AGGTCCCGAA TGGGGAGGCC TACACTGAGA ACTGCAAGAG CGAGCTGAGC 
GCGGGCGAGT TTCCATGTGA AGTCTGTGGC CAGGCCTTTA GTCAAACCTG GTTCCTGAAA 
GCTCACATGA AAAAGCACAG GGGGTCATTT GATCACGGGT GCCACATTTG TGGCAGGAGA 
TTCAAAGAGC CCTGGTTTCT CAAAAACCAC ATGAAGTCGC ACGGCCCCAA GACCGGGAGC 
AAAAACAAAC CAAAAAATGA TTTGGAGCTC ATAGCCACTA TAAATGACGT GATACAGGAG 
GAGACGATTG TGACGGGCCT CTCTCTGTAC GAAGTTTGCA CCAAGTGTGG AAATCTGTTT 
ACAAATATGG AAAGCCTGAA AGCGCATAAC GCTGTTCACT ACCGGGCGCA GCGGGGCAGC 
GCCGGGGATA AAGCCGAGGG CTTAATTGAT GGGAGCCTAA GCTCCTCGGT AACAAAGCAG 
TTTTTCTTGC AGTGTCTCAA TCTAAGGCCA TCTGTAGGGC CGGATAGAGT TACAAGAGGA 
CAGCCTGGGA AAAGAGTAGC CGAGCTGGAT CCGGTCAGTA GCTACCAAGC TTGGCAGCTG 
GCCACCAAAG GAAAAGTAGT AGAGCCCTCA GAGTATGTGA AGTATGTGGG ATGGGACGAG 
GCCCTGGCGG ATGCGGACGT GACTTACGAC AAGGATAAGA GAGAGTATAT CCTTGTCAAC 
CAGGAGAAGC GCAAGCGAGA CCAGGACTCG CCCAGCTCTT CCAGCAACCC CAAGAAGAAG 
AGCTGCACCG GCGGGCGCCC GGAGAAAACC GGCGGTGCCC CGCCGGGGGA GAGCTGCCCG 
CTCGCCCAGG GGGACCTGGA CTACCGCCCT GCCTCACGGC AGAGCCGGCG GGCCGCCCAG 
AACAAGTCCA CCGAGTGCTT TGAGTGCGGG AAGATCTTCC GCACCTACCA CCAAATGGTG 
CTGCACTCCC GGGTGCACCG CAAGGAGCGG AGGAGCTGCG GCGAGGGCGG GACGGCTGCC 
CAGCCCGACC GCTACGGCTC TAGCAGCGAG GAAGGGGACT CGGGGTCCGT CAGCGGGCCC 
AGCACCCCCG GCTCTGCGTC CGCCCAGGAG GACTCGGTCA CCTCCGTCCT GGGGGAGGAG 
GGGGCCGAGG AGAGCTCGGA GGAGGGAGCA GCCAACCCCT CGCTAGATGA AAAGCCATAC 
CACTGCAACT TTTCTGAAGA GGAAACCCCG ATGGTAACAT CTCAGTTGGA TGAACTCCCG 
AACCTGGGAA GCTGCAATGC CAGAGAAAAT GAGGCCAGGG AATCTAAACC AGAGATGCCT 
ACCCTGGTGT CAGCACTGGA GAGTGTCATC AAGGAGCCCT TTTCGAAATA CCAAGATCCA 
AAAGGTTCTG CAGACATAAA GATGCCAGCA TTTCACCACA GCCAAGGGCT TCCTTGCTCC 
AGTCGCATTG CTAGCTTTGC TTCGGGTGTG GGCCAGATAT CTTCAAACAT AGTCATGGAC 
TTGAAAACAT CACTTGAAGT GCAGGCTAGT CGCTCTAGAG AAAATTTGCT AGACTTGCAC 
AGGGAACATC CTCTGACAGA AAAAGGTGCT GAAGCTCAAG AGGTAGCTCC ACTCGATTTG 
AGTGAAAAAT CAACAAGGGA TAATTCATGC AATAAAAATC TGAATACATC CTTGCAGGCT 
GCTTTAATTG TCTACCCATG TCCTTTCTGC AGTCACAAGA CCTACTACCC TGAAGTCCTG 
TGGATGCACA AAAGAATTTT GCACAAAATC AGCTGTAACT CGATGGTCCC CCCTTGGGTG 
CAACAGAATG GATTCAAGAG TATTAAAAAC AACCTGGTCT TTCTGGCAAG GAGTGGGCGT 
ACCGGCCCCC CTCCTGTTCT CGGCGGTAAG GAATGCCAGC CTTTGCCCAT TGCCAGATTT 
ACACGCACTC AAGTGCCAAG TGCATTGCCA GGATCCAAAA GCAGCTCTCT TTCTGTTGGG 
ATGGCTGCAA AGTCCGGGAG TACGCATCAG TCAAAGGACA GTCATGCCTT CGGCCACTGT 
GGTCCACGCT TATCAGGTCT GGATGGGTAC AGACAGCCCA AGTTAAACCA TTCCCAGGAG 
CAATACGGCA TAGCAGCACA ACAAAAAAGT AAATACGAAG CAAATTCCAA ACTTATGCAA 
ATGGGAGCCT ACAGCAGAAG TGTAACGCCT ACTCAAACGG TCATATCCAG GCCTAGCACA 
CAGCCCACCA ACAGTAAGCA AGTGGAAAAG TACGTGGTTC CCCAAGGAAG TACTAGTTTT 
GCCCCTCCAG GTAAGCACTG TGCATCCGAC TCCATGAAGG CCAAATTCAA CCCACCGCCC 
CAGTATCATC CTCTGTGTAA AAGCGAGCAG TACACTAAAC ACGAAGAGCC ATCGGTGCCT 
CAGAGGGATT CTCATGTGAA GGCTGGGAAT GAGATGAGGA CATTGGCAAA CTGCACAGCG 
GGAGCACGAG CGAGTCCACT GCTGCAGCCC CAGCCTAGCG CCGCGGGAGT TTCTCCGGCT 
TTACACTCCA GCAAACAGGA TCTGGGATCA GACGGGCATG AGAAACGTTT GGACGTTTTA 
AATATCTTTA AAACATACAT TCCAAAGGAC CTTGCTTCGT TGTACCAAAC CTGCGGTGCC 
AACAGCCCTG TCCTGGATCA CGCAGGGATG CTCAGGACAC AAACACGCCA AGGAGACTGC 
GTCTGCAGAG AATGTGGAAA ATGCTTTACC CAACCCAGTC ACCTCAGGAC TCATCAGAGG 
TCCCATGCAG TGGTATTTGA GTCTAATGGA CTCCGAGGTA CTGAAGTTCA CACCACCTCC 
GCAGATGCCC CAAAACAAGG GAGAGACCAC GGCGGCGCGG AGGTCGCCCA CACGCTGCCT 
CTCCGGAAGG GAACCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF516 ( XM_011585941.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011584243.1 Aquila chrysaetos canadensis zinc finger protein 516 (ZNF516), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq22802
Clone ID Related Accession (Same CDS sequence)	XM_011585941.1
Accession Version	XM_011585941.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	zinc finger protein 516 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950922.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAA ACGCGGTCCA GCCCTCCTCT GCTGCCGGAC ATGCTTTTGT CCTCCTGCCC 
CGCTCACCCG ATGTTGCAGT AATGCAGATG GATCGTAGTA GAGAGGCAGA AATGGAGTTA 
CGGAGATCCT CCAGCCCAGG CAAGCTAAGC AAGAATGACG TGGATGCTGA CAAGACCATG 
TGCCACAGCT GCTGCATCTG CGGTAAAAGT TTCCCTTTTC AGAGCTCCTT GTCGCAGCAC 
ATGAGGAAGC ACACGGGCGA GAAGCCCTAC AAGTGCCCTT ACTGTGACCA CAGAGCTTCC 
CAAAAGGGCA ACCTGAAGAT CCACATCCGT AGTCACAGAA CAGGCACTTT AAGTCAGGGG 
CATGAGGCGG AGATGGGAGA GGCGCAGCTG GGGGAGATGG GTGTTTCGGA GGGCCTCGGC 
GGGTGCACCA GCCCCACTAA AAGTACGTCC GCCTGCAACA GGATCTTGAA CGGTACGGCT 
CAGGTAGATA GCAGCAAGAT CTTGTTGAGA AGCAGCAAGA AGGAGGTCAT GGAGGCGGCG 
CCAGCCGAGG AGGATGGCAA GCTGACCTCT TACCAGTGCA CCTTCTGCAA AAACAAATTT 
GAAAGGAAGA AGGACTTGGA GCAGCACCTG CACCAGGTCC ACAAACCGTT CAAGTGCAGG 
TTGTGTAGCT ACATGACCCT GAGGGAGGAG ACGCTGTTAA ACCACATAGA GAAGGACCAT 
ATAACAGCTC AGGTCCCGAA TGGGGAGGCC TACACTGAGA ACTGCAAGAG CGAGCTGAGC 
GCGGGCGAGT TTCCATGTGA AGTCTGTGGC CAGGCCTTTA GTCAAACCTG GTTCCTGAAA 
GCTCACATGA AAAAGCACAG GGGGTCATTT GATCACGGGT GCCACATTTG TGGCAGGAGA 
TTCAAAGAGC CCTGGTTTCT CAAAAACCAC ATGAAGTCGC ACGGCCCCAA GACCGGGAGC 
AAAAACAAAC CAAAAAATGA TTTGGAGCTC ATAGCCACTA TAAATGACGT GATACAGGAG 
GAGACGATTG TGACGGGCCT CTCTCTGTAC GAAGTTTGCA CCAAGTGTGG AAATCTGTTT 
ACAAATATGG AAAGCCTGAA AGCGCATAAC GCTGTTCACT ACCGGGCGCA GCGGGGCAGC 
GCCGGGGATA AAGCCGAGGG CTTAATTGAT GGGAGCCTAA GCTCCTCGGT AACAAAGCAG 
TTTTTCTTGC AGTGTCTCAA TCTAAGGCCA TCTGTAGGGC CGGATAGAGT TACAAGAGGA 
CAGCCTGGGA AAAGAGTAGC CGAGCTGGAT CCGGTCAGTA GCTACCAAGC TTGGCAGCTG 
GCCACCAAAG GAAAAGTAGT AGAGCCCTCA GAGTATGTGA AGTATGTGGG ATGGGACGAG 
GCCCTGGCGG ATGCGGACGT GACTTACGAC AAGGATAAGA GAGAGTATAT CCTTGTCAAC 
CAGGAGAAGC GCAAGCGAGA CCAGGACTCG CCCAGCTCTT CCAGCAACCC CAAGAAGAAG 
AGCTGCACCG GCGGGCGCCC GGAGAAAACC GGCGGTGCCC CGCCGGGGGA GAGCTGCCCG 
CTCGCCCAGG GGGACCTGGA CTACCGCCCT GCCTCACGGC AGAGCCGGCG GGCCGCCCAG 
AACAAGTCCA CCGAGTGCTT TGAGTGCGGG AAGATCTTCC GCACCTACCA CCAAATGGTG 
CTGCACTCCC GGGTGCACCG CAAGGAGCGG AGGAGCTGCG GCGAGGGCGG GACGGCTGCC 
CAGCCCGACC GCTACGGCTC TAGCAGCGAG GAAGGGGACT CGGGGTCCGT CAGCGGGCCC 
AGCACCCCCG GCTCTGCGTC CGCCCAGGAG GACTCGGTCA CCTCCGTCCT GGGGGAGGAG 
GGGGCCGAGG AGAGCTCGGA GGAGGGAGCA GCCAACCCCT CGCTAGATGA AAAGCCATAC 
CACTGCAACT TTTCTGAAGA GGAAACCCCG ATGGTAACAT CTCAGTTGGA TGAACTCCCG 
AACCTGGGAA GCTGCAATGC CAGAGAAAAT GAGGCCAGGG AATCTAAACC AGAGATGCCT 
ACCCTGGTGT CAGCACTGGA GAGTGTCATC AAGGAGCCCT TTTCGAAATA CCAAGATCCA 
AAAGGTTCTG CAGACATAAA GATGCCAGCA TTTCACCACA GCCAAGGGCT TCCTTGCTCC 
AGTCGCATTG CTAGCTTTGC TTCGGGTGTG GGCCAGATAT CTTCAAACAT AGTCATGGAC 
TTGAAAACAT CACTTGAAGT GCAGGCTAGT CGCTCTAGAG AAAATTTGCT AGACTTGCAC 
AGGGAACATC CTCTGACAGA AAAAGGTGCT GAAGCTCAAG AGGTAGCTCC ACTCGATTTG 
AGTGAAAAAT CAACAAGGGA TAATTCATGC AATAAAAATC TGAATACATC CTTGCAGGCT 
GCTTTAATTG TCTACCCATG TCCTTTCTGC AGTCACAAGA CCTACTACCC TGAAGTCCTG 
TGGATGCACA AAAGAATTTT GCACAAAATC AGCTGTAACT CGATGGTCCC CCCTTGGGTG 
CAACAGAATG GATTCAAGAG TATTAAAAAC AACCTGGTCT TTCTGGCAAG GAGTGGGCGT 
ACCGGCCCCC CTCCTGTTCT CGGCGGTAAG GAATGCCAGC CTTTGCCCAT TGCCAGATTT 
ACACGCACTC AAGTGCCAAG TGCATTGCCA GGATCCAAAA GCAGCTCTCT TTCTGTTGGG 
ATGGCTGCAA AGTCCGGGAG TACGCATCAG TCAAAGGACA GTCATGCCTT CGGCCACTGT 
GGTCCACGCT TATCAGGTCT GGATGGGTAC AGACAGCCCA AGTTAAACCA TTCCCAGGAG 
CAATACGGCA TAGCAGCACA ACAAAAAAGT AAATACGAAG CAAATTCCAA ACTTATGCAA 
ATGGGAGCCT ACAGCAGAAG TGTAACGCCT ACTCAAACGG TCATATCCAG GCCTAGCACA 
CAGCCCACCA ACAGTAAGCA AGTGGAAAAG TACGTGGTTC CCCAAGGAAG TACTAGTTTT 
GCCCCTCCAG GTAAGCACTG TGCATCCGAC TCCATGAAGG CCAAATTCAA CCCACCGCCC 
CAGTATCATC CTCTGTGTAA AAGCGAGCAG TACACTAAAC ACGAAGAGCC ATCGGTGCCT 
CAGAGGGATT CTCATGTGAA GGCTGGGAAT GAGATGAGGA CATTGGCAAA CTGCACAGCG 
GGAGCACGAG CGAGTCCACT GCTGCAGCCC CAGCCTAGCG CCGCGGGAGT TTCTCCGGCT 
TTACACTCCA GCAAACAGGA TCTGGGATCA GACGGGCATG AGAAACGTTT GGACGTTTTA 
AATATCTTTA AAACATACAT TCCAAAGGAC CTTGCTTCGT TGTACCAAAC CTGCGGTGCC 
AACAGCCCTG TCCTGGATCA CGCAGGGATG CTCAGGACAC AAACACGCCA AGGAGACTGC 
GTCTGCAGAG AATGTGGAAA ATGCTTTACC CAACCCAGTC ACCTCAGGAC TCATCAGAGG 
TCCCATGCAG GGGAGAGACC ACGGCGGCGC GGAGGTCGCC CACACGCTGC CTCTCCGGAA 
GGGAACCTAG AGCCCCGGCG GAGGAACCCC CGTGCCAGCG GCCCCCACCC CGACGGGCAG 
CGCCGGCAGC CCCCCGCGCA GCCGCCCCGC GCCCCGGCGG CTGCGGATCA GCCTCCCCGC 
CAGCCATCGC CTCGCCCGGA GCCGCTGCGC CAGAGTGAAA CCGGCGCTCT GCGAGGAGGG 
GGGTGCCTCT GCGCTGTTAA TCATTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011584243.1
CDS	120..3869
Translation	MIENAVQPSSAAGHAFVLLPRSPDVAVMQMDRSREAEMELRRSSSPGKLSKNDVDADKTMCHSCCICGKSFPFQSSLSQHMRKHTGEKPYKCPYCDHRASQKGNLKIHIRSHRTGTLSQGHEAEMGEAQLGEMGVSEGLGGCTSPTKSTSACNRILNGTAQVDSSKILLRSSKKEVMEAAPAEEDGKLTSYQCTFCKNKFERKKDLEQHLHQVHKPFKCRLCSYMTLREETLLNHIEKDHITAQVPNGEAYTENCKSELSAGEFPCEVCGQAFSQTWFLKAHMKKHRGSFDHGCHICGRRFKEPWFLKNHMKSHGPKTGSKNKPKNDLELIATINDVIQEETIVTGLSLYEVCTKCGNLFTNMESLKAHNAVHYRAQRGSAGDKAEGLIDGSLSSSVTKQFFLQCLNLRPSVGPDRVTRGQPGKRVAELDPVSSYQAWQLATKGKVVEPSEYVKYVGWDEALADADVTYDKDKREYILVNQEKRKRDQDSPSSSSNPKKKSCTGGRPEKTGGAPPGESCPLAQGDLDYRPASRQSRRAAQNKSTECFECGKIFRTYHQMVLHSRVHRKERRSCGEGGTAAQPDRYGSSSEEGDSGSVSGPSTPGSASAQEDSVTSVLGEEGAEESSEEGAANPSLDEKPYHCNFSEEETPMVTSQLDELPNLGSCNARENEARESKPEMPTLVSALESVIKEPFSKYQDPKGSADIKMPAFHHSQGLPCSSRIASFASGVGQISSNIVMDLKTSLEVQASRSRENLLDLHREHPLTEKGAEAQEVAPLDLSEKSTRDNSCNKNLNTSLQAALIVYPCPFCSHKTYYPEVLWMHKRILHKISCNSMVPPWVQQNGFKSIKNNLVFLARSGRTGPPPVLGGKECQPLPIARFTRTQVPSALPGSKSSSLSVGMAAKSGSTHQSKDSHAFGHCGPRLSGLDGYRQPKLNHSQEQYGIAAQQKSKYEANSKLMQMGAYSRSVTPTQTVISRPSTQPTNSKQVEKYVVPQGSTSFAPPGKHCASDSMKAKFNPPPQYHPLCKSEQYTKHEEPSVPQRDSHVKAGNEMRTLANCTAGARASPLLQPQPSAAGVSPALHSSKQDLGSDGHEKRLDVLNIFKTYIPKDLASLYQTCGANSPVLDHAGMLRTQTRQGDCVCRECGKCFTQPSHLRTHQRSHAGERPRRRGGRPHAASPEGNLEPRRRNPRASGPHPDGQRRQPPAQPPRAPAAADQPPRQPSPRPEPLRQSETGALRGGGCLCAVNHF

Target ORF information:

RefSeq Version	XM_011585941.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis zinc finger protein 516 (ZNF516), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011585941.1

ORF Insert Sequence:

ATGATTGAAA ACGCGGTCCA GCCCTCCTCT GCTGCCGGAC ATGCTTTTGT CCTCCTGCCC 
CGCTCACCCG ATGTTGCAGT AATGCAGATG GATCGTAGTA GAGAGGCAGA AATGGAGTTA 
CGGAGATCCT CCAGCCCAGG CAAGCTAAGC AAGAATGACG TGGATGCTGA CAAGACCATG 
TGCCACAGCT GCTGCATCTG CGGTAAAAGT TTCCCTTTTC AGAGCTCCTT GTCGCAGCAC 
ATGAGGAAGC ACACGGGCGA GAAGCCCTAC AAGTGCCCTT ACTGTGACCA CAGAGCTTCC 
CAAAAGGGCA ACCTGAAGAT CCACATCCGT AGTCACAGAA CAGGCACTTT AAGTCAGGGG 
CATGAGGCGG AGATGGGAGA GGCGCAGCTG GGGGAGATGG GTGTTTCGGA GGGCCTCGGC 
GGGTGCACCA GCCCCACTAA AAGTACGTCC GCCTGCAACA GGATCTTGAA CGGTACGGCT 
CAGGTAGATA GCAGCAAGAT CTTGTTGAGA AGCAGCAAGA AGGAGGTCAT GGAGGCGGCG 
CCAGCCGAGG AGGATGGCAA GCTGACCTCT TACCAGTGCA CCTTCTGCAA AAACAAATTT 
GAAAGGAAGA AGGACTTGGA GCAGCACCTG CACCAGGTCC ACAAACCGTT CAAGTGCAGG 
TTGTGTAGCT ACATGACCCT GAGGGAGGAG ACGCTGTTAA ACCACATAGA GAAGGACCAT 
ATAACAGCTC AGGTCCCGAA TGGGGAGGCC TACACTGAGA ACTGCAAGAG CGAGCTGAGC 
GCGGGCGAGT TTCCATGTGA AGTCTGTGGC CAGGCCTTTA GTCAAACCTG GTTCCTGAAA 
GCTCACATGA AAAAGCACAG GGGGTCATTT GATCACGGGT GCCACATTTG TGGCAGGAGA 
TTCAAAGAGC CCTGGTTTCT CAAAAACCAC ATGAAGTCGC ACGGCCCCAA GACCGGGAGC 
AAAAACAAAC CAAAAAATGA TTTGGAGCTC ATAGCCACTA TAAATGACGT GATACAGGAG 
GAGACGATTG TGACGGGCCT CTCTCTGTAC GAAGTTTGCA CCAAGTGTGG AAATCTGTTT 
ACAAATATGG AAAGCCTGAA AGCGCATAAC GCTGTTCACT ACCGGGCGCA GCGGGGCAGC 
GCCGGGGATA AAGCCGAGGG CTTAATTGAT GGGAGCCTAA GCTCCTCGGT AACAAAGCAG 
TTTTTCTTGC AGTGTCTCAA TCTAAGGCCA TCTGTAGGGC CGGATAGAGT TACAAGAGGA 
CAGCCTGGGA AAAGAGTAGC CGAGCTGGAT CCGGTCAGTA GCTACCAAGC TTGGCAGCTG 
GCCACCAAAG GAAAAGTAGT AGAGCCCTCA GAGTATGTGA AGTATGTGGG ATGGGACGAG 
GCCCTGGCGG ATGCGGACGT GACTTACGAC AAGGATAAGA GAGAGTATAT CCTTGTCAAC 
CAGGAGAAGC GCAAGCGAGA CCAGGACTCG CCCAGCTCTT CCAGCAACCC CAAGAAGAAG 
AGCTGCACCG GCGGGCGCCC GGAGAAAACC GGCGGTGCCC CGCCGGGGGA GAGCTGCCCG 
CTCGCCCAGG GGGACCTGGA CTACCGCCCT GCCTCACGGC AGAGCCGGCG GGCCGCCCAG 
AACAAGTCCA CCGAGTGCTT TGAGTGCGGG AAGATCTTCC GCACCTACCA CCAAATGGTG 
CTGCACTCCC GGGTGCACCG CAAGGAGCGG AGGAGCTGCG GCGAGGGCGG GACGGCTGCC 
CAGCCCGACC GCTACGGCTC TAGCAGCGAG GAAGGGGACT CGGGGTCCGT CAGCGGGCCC 
AGCACCCCCG GCTCTGCGTC CGCCCAGGAG GACTCGGTCA CCTCCGTCCT GGGGGAGGAG 
GGGGCCGAGG AGAGCTCGGA GGAGGGAGCA GCCAACCCCT CGCTAGATGA AAAGCCATAC 
CACTGCAACT TTTCTGAAGA GGAAACCCCG ATGGTAACAT CTCAGTTGGA TGAACTCCCG 
AACCTGGGAA GCTGCAATGC CAGAGAAAAT GAGGCCAGGG AATCTAAACC AGAGATGCCT 
ACCCTGGTGT CAGCACTGGA GAGTGTCATC AAGGAGCCCT TTTCGAAATA CCAAGATCCA 
AAAGGTTCTG CAGACATAAA GATGCCAGCA TTTCACCACA GCCAAGGGCT TCCTTGCTCC 
AGTCGCATTG CTAGCTTTGC TTCGGGTGTG GGCCAGATAT CTTCAAACAT AGTCATGGAC 
TTGAAAACAT CACTTGAAGT GCAGGCTAGT CGCTCTAGAG AAAATTTGCT AGACTTGCAC 
AGGGAACATC CTCTGACAGA AAAAGGTGCT GAAGCTCAAG AGGTAGCTCC ACTCGATTTG 
AGTGAAAAAT CAACAAGGGA TAATTCATGC AATAAAAATC TGAATACATC CTTGCAGGCT 
GCTTTAATTG TCTACCCATG TCCTTTCTGC AGTCACAAGA CCTACTACCC TGAAGTCCTG 
TGGATGCACA AAAGAATTTT GCACAAAATC AGCTGTAACT CGATGGTCCC CCCTTGGGTG 
CAACAGAATG GATTCAAGAG TATTAAAAAC AACCTGGTCT TTCTGGCAAG GAGTGGGCGT 
ACCGGCCCCC CTCCTGTTCT CGGCGGTAAG GAATGCCAGC CTTTGCCCAT TGCCAGATTT 
ACACGCACTC AAGTGCCAAG TGCATTGCCA GGATCCAAAA GCAGCTCTCT TTCTGTTGGG 
ATGGCTGCAA AGTCCGGGAG TACGCATCAG TCAAAGGACA GTCATGCCTT CGGCCACTGT 
GGTCCACGCT TATCAGGTCT GGATGGGTAC AGACAGCCCA AGTTAAACCA TTCCCAGGAG 
CAATACGGCA TAGCAGCACA ACAAAAAAGT AAATACGAAG CAAATTCCAA ACTTATGCAA 
ATGGGAGCCT ACAGCAGAAG TGTAACGCCT ACTCAAACGG TCATATCCAG GCCTAGCACA 
CAGCCCACCA ACAGTAAGCA AGTGGAAAAG TACGTGGTTC CCCAAGGAAG TACTAGTTTT 
GCCCCTCCAG GTAAGCACTG TGCATCCGAC TCCATGAAGG CCAAATTCAA CCCACCGCCC 
CAGTATCATC CTCTGTGTAA AAGCGAGCAG TACACTAAAC ACGAAGAGCC ATCGGTGCCT 
CAGAGGGATT CTCATGTGAA GGCTGGGAAT GAGATGAGGA CATTGGCAAA CTGCACAGCG 
GGAGCACGAG CGAGTCCACT GCTGCAGCCC CAGCCTAGCG CCGCGGGAGT TTCTCCGGCT 
TTACACTCCA GCAAACAGGA TCTGGGATCA GACGGGCATG AGAAACGTTT GGACGTTTTA 
AATATCTTTA AAACATACAT TCCAAAGGAC CTTGCTTCGT TGTACCAAAC CTGCGGTGCC 
AACAGCCCTG TCCTGGATCA CGCAGGGATG CTCAGGACAC AAACACGCCA AGGAGACTGC 
GTCTGCAGAG AATGTGGAAA ATGCTTTACC CAACCCAGTC ACCTCAGGAC TCATCAGAGG 
TCCCATGCAG GGGAGAGACC ACGGCGGCGC GGAGGTCGCC CACACGCTGC CTCTCCGGAA 
GGGAACCTAG AGCCCCGGCG GAGGAACCCC CGTGCCAGCG GCCCCCACCC CGACGGGCAG 
CGCCGGCAGC CCCCCGCGCA GCCGCCCCGC GCCCCGGCGG CTGCGGATCA GCCTCCCCGC 
CAGCCATCGC CTCGCCCGGA GCCGCTGCGC CAGAGTGAAA CCGGCGCTCT GCGAGGAGGG 
GGGTGCCTCT GCGCTGTTAA TCATTTTTAA