VPS53 cDNA ORF clone, Aquila chrysaetos canadensis

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS53
Entrez Gene ID	105404709
Full Name	VPS53, GARP complex subunit
Gene Type	protein-coding
Organism	Aquila chrysaetos canadensis

mRNA and Protein(s)

mRNA	Protein	Name
XM_011581835.1	XP_011580137.1	vacuolar protein sorting-associated protein 53 homolog isoform X1
XM_011581836.1	XP_011580138.1	vacuolar protein sorting-associated protein 53 homolog isoform X2

Pathways from BioSystems
Gene Ontology

The following VPS53 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS53 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAq19790	XM_011581835.1 Latest version!	Aquila chrysaetos canadensis vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OAq19791	XM_011581836.1 Latest version!	Aquila chrysaetos canadensis vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS53 ( XM_011581835.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011580137.1 Aquila chrysaetos canadensis vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAq19790
Clone ID Related Accession (Same CDS sequence)	XM_011581835.1
Accession Version	XM_011581835.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	vacuolar protein sorting-associated protein 53 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGG ACGAGCCGGA GCTGGCGGCC GGGTTGGAGG CGGCTCTGGA GCTGGCGCCC 
GCCGTGCAGG CCGCCATCGA GCAGGTATTT CCCAGCCAAG ATCCACTGGA CAGAGCAGAT 
TTCAATGCTG TGGAATATAT TAATACTCTC TTTCCCACTG AGCAATCTTT GGCGAACATC 
GATGAGGTTG TGAACAAAAT CAGATTGAAA ATAAGGAGGT TGGATGACAA CATTCGAACT 
GTAGTGAGAG GACAGACCAA CGTGGGACAG GATGGCAGGC AGGCCCTGGA AGAAGCCCAG 
AAAGCTATTC AACAGCTCTT TGGTAAAATT AAGGATATTA AAGACAAGGC TGAAAAATCA 
GAACAAATGG TTAAAGAAAT CACACGGGAT ATCAAGCAGC TAGATCATGC CAAGCGTCAT 
CTGACCACCT CCATCACCAC CCTCAACCAC CTACACATGC TGGCAGGGGG TGTGGATTCA 
CTGGAGGCTA TGACCAGGAG GAGACAATAT GGGGAAGTTG CCAACCTTCT CCAAGGTGTC 
GTGAATGTGT TAGAGCACTT CAACAAATAC ATGGGGATTC CTCAGATTCG ACAGCTTTCC 
GAAAGGGTGA AGGCAGCACA GAATGAGTTG GGACAGCAGA TCCTGGCTGA CTTCGAGGAA 
GCTTTTCCTT CTCAAGGTAC AAAGAGGCCT GGTGGACCCA GCAATGTCCT ACGTGATGCG 
TGTCTAGTCG CCAATGTCCT GGATCCCAGA ATCAAACAGG AAATCATCAA GAAATTTATT 
AAACAACACC TCTCAGAGTA TCTGGTCCTT TTCCAGGAAA ACCAAGATGT GGCCTGGCTG 
GATAAGATTG ACAGGCGCTA TGCTTGGATA AAGCGTCAAC TGGTGGACTA CGAGGAGAAA 
TACGGGCGCA TGTTTCCCCA GGAGTGGTGT ATGACGGAGC GCATCGCTGT GGAGTTCTGC 
CACGTCACAA GAACAGAGCT TGCTAAGATA ATGCGCACAA GAGCAAAGGA GATTGAGGTG 
AAATTGCTTT TGTTTGCAAT TCAGAGGACA ACCAACTTTG AAGGACTTCT GGCTAAACGC 
TTCTCAGGTT GCACTCTAGC TGATGGGACA GTGAAGAAGC CAGAAGTGCC ACTTCCCTCC 
ACCAACCCGT TCCTGGAGGA TGAAACTGGG ACAGAGACAG ATGAGATTGT GATCGAGAAA 
AGTGATACGG ACAAACCAAA GAAGCCGAAG GTCCCTGACA ATCCTTTTCA TGGCATTGTT 
TCCAAGTGCT TTGAGCCTCA CCTTTATGTG TATATCGAGT CCCAGGACAA AAATCTTGGC 
GAACTGATTG ACAGGTTTGT GGCTGACTTC AAGGCTCAAG GTCCCCCCAA ACCCAATGTG 
GACGAAGGAG GAGCCGTCCT GCCCAGCTGT GCTGACCTCT TTGTGTACTA CAAGAAGTGC 
ATGGTGCAGT GCTCCCAGCT CAGCACAGGC GAGCCGATGA TAGCCCTGAC CACCATATTC 
CAGAAGTACC TTCGGGAATA CGCCTGGAAA ATTCTGTCTG GAAACCTGCC CAAGACAACT 
AGCAGCAGCG GTGGGCTCAC CATCACTAGT TTGCTGAAAG AAAAGGAAGG CTCTGAAGTG 
GCAAAGTTTA CTTTAGAAGA ACTCTGCCTC ATTTGCAGCA TCCTAAGTAC TGCAGAGTAC 
TGCTTGGCGA CCACCCAGCA GTTGGAAGAG AAACTCAAAG AAAAAGTGGA TGCAAGTCTA 
GTGGAGAGAA TCAACCTGAC GGGAGAGATG GACACTTTCA GCATTGTAAT CTCCAACAGC 
ATACAGCTGT TAGTGCAGGA CCTGGATGCA GCCTGTGACC CTGCCCTGAC TGCTATGAGC 
AAGATGCAGT GGCAGAACGT GGAACACGTT GGTGACCAGA GTCCTTATGT CACCTCAGTG 
ATTCTCCACA TCAAACAGAA CGTCCCCATC ATCCGCGACA ATCTGGCCTC CACGAGAAAG 
TATTTCACTC AGTTCTGTAT AAAATTTGCA AACTCCTTCA TTCCCAAGTT CATTAACCAT 
CTCTTCAAGT GCAAACCTAT TAGCATGGTG GGCGCAGAAC AGCTGCTGCT GGACACTCAC 
TCTCTGAAGA TGGTTCTCCT GGATCTGCCC TCCATTGGGT CCCAAGTGGT GAGGAAGGCT 
CCTGCCAGCT ACACAAAGAT AGTGGTGAAA GGCATGACTC GGGCTGAAAT GATCCTGAAG 
GTGGTTATGG CTCCTCACGA GCCCCCGGTT GTTTTTGTCG ACAATTACAT CAAGCTCCTT 
GCTGACTGCA GCACTGACAC TTTCCAGAAG ATACTAGACA TGAAGGGCCT GAAGAGGAGT 
GAACAAAGCA GCATGCTGGA CCTCTTCCGC CAGCGCCTCC CGGCTCCCCC CTCCGGAGTG 
GAGAACACCG GCTCCCTCTC GCTGAGCGCT CCTACGCCCG AGCAGGAATC CTCTCGGATA 
CGGAAACTGG AGAAACTCAT CAAAAAAAGA CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011580137.1
CDS	12..2507
Translation	MEEDEPELAAGLEAALELAPAVQAAIEQVFPSQDPLDRADFNAVEYINTLFPTEQSLANIDEVVNKIRLKIRRLDDNIRTVVRGQTNVGQDGRQALEEAQKAIQQLFGKIKDIKDKAEKSEQMVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVVNVLEHFNKYMGIPQIRQLSERVKAAQNELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANVLDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPQEWCMTERIAVEFCHVTRTELAKIMRTRAKEIEVKLLLFAIQRTTNFEGLLAKRFSGCTLADGTVKKPEVPLPSTNPFLEDETGTETDEIVIEKSDTDKPKKPKVPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNVDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTSSSGGLTITSLLKEKEGSEVAKFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDASLVERINLTGEMDTFSIVISNSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFINHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPPVVFVDNYIKLLADCSTDTFQKILDMKGLKRSEQSSMLDLFRQRLPAPPSGVENTGSLSLSAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_011581835.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011581835.1

ORF Insert Sequence:

ATGGAGGAGG ACGAGCCGGA GCTGGCGGCC GGGTTGGAGG CGGCTCTGGA GCTGGCGCCC 
GCCGTGCAGG CCGCCATCGA GCAGGTATTT CCCAGCCAAG ATCCACTGGA CAGAGCAGAT 
TTCAATGCTG TGGAATATAT TAATACTCTC TTTCCCACTG AGCAATCTTT GGCGAACATC 
GATGAGGTTG TGAACAAAAT CAGATTGAAA ATAAGGAGGT TGGATGACAA CATTCGAACT 
GTAGTGAGAG GACAGACCAA CGTGGGACAG GATGGCAGGC AGGCCCTGGA AGAAGCCCAG 
AAAGCTATTC AACAGCTCTT TGGTAAAATT AAGGATATTA AAGACAAGGC TGAAAAATCA 
GAACAAATGG TTAAAGAAAT CACACGGGAT ATCAAGCAGC TAGATCATGC CAAGCGTCAT 
CTGACCACCT CCATCACCAC CCTCAACCAC CTACACATGC TGGCAGGGGG TGTGGATTCA 
CTGGAGGCTA TGACCAGGAG GAGACAATAT GGGGAAGTTG CCAACCTTCT CCAAGGTGTC 
GTGAATGTGT TAGAGCACTT CAACAAATAC ATGGGGATTC CTCAGATTCG ACAGCTTTCC 
GAAAGGGTGA AGGCAGCACA GAATGAGTTG GGACAGCAGA TCCTGGCTGA CTTCGAGGAA 
GCTTTTCCTT CTCAAGGTAC AAAGAGGCCT GGTGGACCCA GCAATGTCCT ACGTGATGCG 
TGTCTAGTCG CCAATGTCCT GGATCCCAGA ATCAAACAGG AAATCATCAA GAAATTTATT 
AAACAACACC TCTCAGAGTA TCTGGTCCTT TTCCAGGAAA ACCAAGATGT GGCCTGGCTG 
GATAAGATTG ACAGGCGCTA TGCTTGGATA AAGCGTCAAC TGGTGGACTA CGAGGAGAAA 
TACGGGCGCA TGTTTCCCCA GGAGTGGTGT ATGACGGAGC GCATCGCTGT GGAGTTCTGC 
CACGTCACAA GAACAGAGCT TGCTAAGATA ATGCGCACAA GAGCAAAGGA GATTGAGGTG 
AAATTGCTTT TGTTTGCAAT TCAGAGGACA ACCAACTTTG AAGGACTTCT GGCTAAACGC 
TTCTCAGGTT GCACTCTAGC TGATGGGACA GTGAAGAAGC CAGAAGTGCC ACTTCCCTCC 
ACCAACCCGT TCCTGGAGGA TGAAACTGGG ACAGAGACAG ATGAGATTGT GATCGAGAAA 
AGTGATACGG ACAAACCAAA GAAGCCGAAG GTCCCTGACA ATCCTTTTCA TGGCATTGTT 
TCCAAGTGCT TTGAGCCTCA CCTTTATGTG TATATCGAGT CCCAGGACAA AAATCTTGGC 
GAACTGATTG ACAGGTTTGT GGCTGACTTC AAGGCTCAAG GTCCCCCCAA ACCCAATGTG 
GACGAAGGAG GAGCCGTCCT GCCCAGCTGT GCTGACCTCT TTGTGTACTA CAAGAAGTGC 
ATGGTGCAGT GCTCCCAGCT CAGCACAGGC GAGCCGATGA TAGCCCTGAC CACCATATTC 
CAGAAGTACC TTCGGGAATA CGCCTGGAAA ATTCTGTCTG GAAACCTGCC CAAGACAACT 
AGCAGCAGCG GTGGGCTCAC CATCACTAGT TTGCTGAAAG AAAAGGAAGG CTCTGAAGTG 
GCAAAGTTTA CTTTAGAAGA ACTCTGCCTC ATTTGCAGCA TCCTAAGTAC TGCAGAGTAC 
TGCTTGGCGA CCACCCAGCA GTTGGAAGAG AAACTCAAAG AAAAAGTGGA TGCAAGTCTA 
GTGGAGAGAA TCAACCTGAC GGGAGAGATG GACACTTTCA GCATTGTAAT CTCCAACAGC 
ATACAGCTGT TAGTGCAGGA CCTGGATGCA GCCTGTGACC CTGCCCTGAC TGCTATGAGC 
AAGATGCAGT GGCAGAACGT GGAACACGTT GGTGACCAGA GTCCTTATGT CACCTCAGTG 
ATTCTCCACA TCAAACAGAA CGTCCCCATC ATCCGCGACA ATCTGGCCTC CACGAGAAAG 
TATTTCACTC AGTTCTGTAT AAAATTTGCA AACTCCTTCA TTCCCAAGTT CATTAACCAT 
CTCTTCAAGT GCAAACCTAT TAGCATGGTG GGCGCAGAAC AGCTGCTGCT GGACACTCAC 
TCTCTGAAGA TGGTTCTCCT GGATCTGCCC TCCATTGGGT CCCAAGTGGT GAGGAAGGCT 
CCTGCCAGCT ACACAAAGAT AGTGGTGAAA GGCATGACTC GGGCTGAAAT GATCCTGAAG 
GTGGTTATGG CTCCTCACGA GCCCCCGGTT GTTTTTGTCG ACAATTACAT CAAGCTCCTT 
GCTGACTGCA GCACTGACAC TTTCCAGAAG ATACTAGACA TGAAGGGCCT GAAGAGGAGT 
GAACAAAGCA GCATGCTGGA CCTCTTCCGC CAGCGCCTCC CGGCTCCCCC CTCCGGAGTG 
GAGAACACCG GCTCCCTCTC GCTGAGCGCT CCTACGCCCG AGCAGGAATC CTCTCGGATA 
CGGAAACTGG AGAAACTCAT CAAAAAAAGA CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS53 ( XM_011581836.1 ) cDNA ORF clone, Aquila chrysaetos canadensis -> XP_011580138.1 Aquila chrysaetos canadensis vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAq19791
Clone ID Related Accession (Same CDS sequence)	XM_011581836.1
Accession Version	XM_011581836.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-12
Organism	Aquila chrysaetos canadensis
Product	vacuolar protein sorting-associated protein 53 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_011950904.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Aquila chrysaetos canadensis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTAAAG AAATCACACG GGATATCAAG CAGCTAGATC ATGCCAAGCG TCATCTGACC 
ACCTCCATCA CCACCCTCAA CCACCTACAC ATGCTGGCAG GGGGTGTGGA TTCACTGGAG 
GCTATGACCA GGAGGAGACA ATATGGGGAA GTTGCCAACC TTCTCCAAGG TGTCGTGAAT 
GTGTTAGAGC ACTTCAACAA ATACATGGGG ATTCCTCAGA TTCGACAGCT TTCCGAAAGG 
GTGAAGGCAG CACAGAATGA GTTGGGACAG CAGATCCTGG CTGACTTCGA GGAAGCTTTT 
CCTTCTCAAG GTACAAAGAG GCCTGGTGGA CCCAGCAATG TCCTACGTGA TGCGTGTCTA 
GTCGCCAATG TCCTGGATCC CAGAATCAAA CAGGAAATCA TCAAGAAATT TATTAAACAA 
CACCTCTCAG AGTATCTGGT CCTTTTCCAG GAAAACCAAG ATGTGGCCTG GCTGGATAAG 
ATTGACAGGC GCTATGCTTG GATAAAGCGT CAACTGGTGG ACTACGAGGA GAAATACGGG 
CGCATGTTTC CCCAGGAGTG GTGTATGACG GAGCGCATCG CTGTGGAGTT CTGCCACGTC 
ACAAGAACAG AGCTTGCTAA GATAATGCGC ACAAGAGCAA AGGAGATTGA GGTGAAATTG 
CTTTTGTTTG CAATTCAGAG GACAACCAAC TTTGAAGGAC TTCTGGCTAA ACGCTTCTCA 
GGTTGCACTC TAGCTGATGG GACAGTGAAG AAGCCAGAAG TGCCACTTCC CTCCACCAAC 
CCGTTCCTGG AGGATGAAAC TGGGACAGAG ACAGATGAGA TTGTGATCGA GAAAAGTGAT 
ACGGACAAAC CAAAGAAGCC GAAGGTCCCT GACAATCCTT TTCATGGCAT TGTTTCCAAG 
TGCTTTGAGC CTCACCTTTA TGTGTATATC GAGTCCCAGG ACAAAAATCT TGGCGAACTG 
ATTGACAGGT TTGTGGCTGA CTTCAAGGCT CAAGGTCCCC CCAAACCCAA TGTGGACGAA 
GGAGGAGCCG TCCTGCCCAG CTGTGCTGAC CTCTTTGTGT ACTACAAGAA GTGCATGGTG 
CAGTGCTCCC AGCTCAGCAC AGGCGAGCCG ATGATAGCCC TGACCACCAT ATTCCAGAAG 
TACCTTCGGG AATACGCCTG GAAAATTCTG TCTGGAAACC TGCCCAAGAC AACTAGCAGC 
AGCGGTGGGC TCACCATCAC TAGTTTGCTG AAAGAAAAGG AAGGCTCTGA AGTGGCAAAG 
TTTACTTTAG AAGAACTCTG CCTCATTTGC AGCATCCTAA GTACTGCAGA GTACTGCTTG 
GCGACCACCC AGCAGTTGGA AGAGAAACTC AAAGAAAAAG TGGATGCAAG TCTAGTGGAG 
AGAATCAACC TGACGGGAGA GATGGACACT TTCAGCATTG TAATCTCCAA CAGCATACAG 
CTGTTAGTGC AGGACCTGGA TGCAGCCTGT GACCCTGCCC TGACTGCTAT GAGCAAGATG 
CAGTGGCAGA ACGTGGAACA CGTTGGTGAC CAGAGTCCTT ATGTCACCTC AGTGATTCTC 
CACATCAAAC AGAACGTCCC CATCATCCGC GACAATCTGG CCTCCACGAG AAAGTATTTC 
ACTCAGTTCT GTATAAAATT TGCAAACTCC TTCATTCCCA AGTTCATTAA CCATCTCTTC 
AAGTGCAAAC CTATTAGCAT GGTGGGCGCA GAACAGCTGC TGCTGGACAC TCACTCTCTG 
AAGATGGTTC TCCTGGATCT GCCCTCCATT GGGTCCCAAG TGGTGAGGAA GGCTCCTGCC 
AGCTACACAA AGATAGTGGT GAAAGGCATG ACTCGGGCTG AAATGATCCT GAAGGTGGTT 
ATGGCTCCTC ACGAGCCCCC GGTTGTTTTT GTCGACAATT ACATCAAGCT CCTTGCTGAC 
TGCAGCACTG ACACTTTCCA GAAGATACTA GACATGAAGG GCCTGAAGAG GAGTGAACAA 
AGCAGCATGC TGGACCTCTT CCGCCAGCGC CTCCCGGCTC CCCCCTCCGG AGTGGAGAAC 
ACCGGCTCCC TCTCGCTGAG CGCTCCTACG CCCGAGCAGG AATCCTCTCG GATACGGAAA 
CTGGAGAAAC TCATCAAAAA AAGACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011580138.1
CDS	337..2466
Translation	MVKEITRDIKQLDHAKRHLTTSITTLNHLHMLAGGVDSLEAMTRRRQYGEVANLLQGVVNVLEHFNKYMGIPQIRQLSERVKAAQNELGQQILADFEEAFPSQGTKRPGGPSNVLRDACLVANVLDPRIKQEIIKKFIKQHLSEYLVLFQENQDVAWLDKIDRRYAWIKRQLVDYEEKYGRMFPQEWCMTERIAVEFCHVTRTELAKIMRTRAKEIEVKLLLFAIQRTTNFEGLLAKRFSGCTLADGTVKKPEVPLPSTNPFLEDETGTETDEIVIEKSDTDKPKKPKVPDNPFHGIVSKCFEPHLYVYIESQDKNLGELIDRFVADFKAQGPPKPNVDEGGAVLPSCADLFVYYKKCMVQCSQLSTGEPMIALTTIFQKYLREYAWKILSGNLPKTTSSSGGLTITSLLKEKEGSEVAKFTLEELCLICSILSTAEYCLATTQQLEEKLKEKVDASLVERINLTGEMDTFSIVISNSIQLLVQDLDAACDPALTAMSKMQWQNVEHVGDQSPYVTSVILHIKQNVPIIRDNLASTRKYFTQFCIKFANSFIPKFINHLFKCKPISMVGAEQLLLDTHSLKMVLLDLPSIGSQVVRKAPASYTKIVVKGMTRAEMILKVVMAPHEPPVVFVDNYIKLLADCSTDTFQKILDMKGLKRSEQSSMLDLFRQRLPAPPSGVENTGSLSLSAPTPEQESSRIRKLEKLIKKRL

Target ORF information:

RefSeq Version	XM_011581836.1
Organism	Aquila chrysaetos canadensis
Definition	Aquila chrysaetos canadensis vacuolar protein sorting 53 homolog (S. cerevisiae) (VPS53), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011581836.1

ORF Insert Sequence:

ATGGTTAAAG AAATCACACG GGATATCAAG CAGCTAGATC ATGCCAAGCG TCATCTGACC 
ACCTCCATCA CCACCCTCAA CCACCTACAC ATGCTGGCAG GGGGTGTGGA TTCACTGGAG 
GCTATGACCA GGAGGAGACA ATATGGGGAA GTTGCCAACC TTCTCCAAGG TGTCGTGAAT 
GTGTTAGAGC ACTTCAACAA ATACATGGGG ATTCCTCAGA TTCGACAGCT TTCCGAAAGG 
GTGAAGGCAG CACAGAATGA GTTGGGACAG CAGATCCTGG CTGACTTCGA GGAAGCTTTT 
CCTTCTCAAG GTACAAAGAG GCCTGGTGGA CCCAGCAATG TCCTACGTGA TGCGTGTCTA 
GTCGCCAATG TCCTGGATCC CAGAATCAAA CAGGAAATCA TCAAGAAATT TATTAAACAA 
CACCTCTCAG AGTATCTGGT CCTTTTCCAG GAAAACCAAG ATGTGGCCTG GCTGGATAAG 
ATTGACAGGC GCTATGCTTG GATAAAGCGT CAACTGGTGG ACTACGAGGA GAAATACGGG 
CGCATGTTTC CCCAGGAGTG GTGTATGACG GAGCGCATCG CTGTGGAGTT CTGCCACGTC 
ACAAGAACAG AGCTTGCTAA GATAATGCGC ACAAGAGCAA AGGAGATTGA GGTGAAATTG 
CTTTTGTTTG CAATTCAGAG GACAACCAAC TTTGAAGGAC TTCTGGCTAA ACGCTTCTCA 
GGTTGCACTC TAGCTGATGG GACAGTGAAG AAGCCAGAAG TGCCACTTCC CTCCACCAAC 
CCGTTCCTGG AGGATGAAAC TGGGACAGAG ACAGATGAGA TTGTGATCGA GAAAAGTGAT 
ACGGACAAAC CAAAGAAGCC GAAGGTCCCT GACAATCCTT TTCATGGCAT TGTTTCCAAG 
TGCTTTGAGC CTCACCTTTA TGTGTATATC GAGTCCCAGG ACAAAAATCT TGGCGAACTG 
ATTGACAGGT TTGTGGCTGA CTTCAAGGCT CAAGGTCCCC CCAAACCCAA TGTGGACGAA 
GGAGGAGCCG TCCTGCCCAG CTGTGCTGAC CTCTTTGTGT ACTACAAGAA GTGCATGGTG 
CAGTGCTCCC AGCTCAGCAC AGGCGAGCCG ATGATAGCCC TGACCACCAT ATTCCAGAAG 
TACCTTCGGG AATACGCCTG GAAAATTCTG TCTGGAAACC TGCCCAAGAC AACTAGCAGC 
AGCGGTGGGC TCACCATCAC TAGTTTGCTG AAAGAAAAGG AAGGCTCTGA AGTGGCAAAG 
TTTACTTTAG AAGAACTCTG CCTCATTTGC AGCATCCTAA GTACTGCAGA GTACTGCTTG 
GCGACCACCC AGCAGTTGGA AGAGAAACTC AAAGAAAAAG TGGATGCAAG TCTAGTGGAG 
AGAATCAACC TGACGGGAGA GATGGACACT TTCAGCATTG TAATCTCCAA CAGCATACAG 
CTGTTAGTGC AGGACCTGGA TGCAGCCTGT GACCCTGCCC TGACTGCTAT GAGCAAGATG 
CAGTGGCAGA ACGTGGAACA CGTTGGTGAC CAGAGTCCTT ATGTCACCTC AGTGATTCTC 
CACATCAAAC AGAACGTCCC CATCATCCGC GACAATCTGG CCTCCACGAG AAAGTATTTC 
ACTCAGTTCT GTATAAAATT TGCAAACTCC TTCATTCCCA AGTTCATTAA CCATCTCTTC 
AAGTGCAAAC CTATTAGCAT GGTGGGCGCA GAACAGCTGC TGCTGGACAC TCACTCTCTG 
AAGATGGTTC TCCTGGATCT GCCCTCCATT GGGTCCCAAG TGGTGAGGAA GGCTCCTGCC 
AGCTACACAA AGATAGTGGT GAAAGGCATG ACTCGGGCTG AAATGATCCT GAAGGTGGTT 
ATGGCTCCTC ACGAGCCCCC GGTTGTTTTT GTCGACAATT ACATCAAGCT CCTTGCTGAC 
TGCAGCACTG ACACTTTCCA GAAGATACTA GACATGAAGG GCCTGAAGAG GAGTGAACAA 
AGCAGCATGC TGGACCTCTT CCGCCAGCGC CTCCCGGCTC CCCCCTCCGG AGTGGAGAAC 
ACCGGCTCCC TCTCGCTGAG CGCTCCTACG CCCGAGCAGG AATCCTCTCG GATACGGAAA 
CTGGAGAAAC TCATCAAAAA AAGACTCTAA