LOC109729009 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109729009
Entrez Gene ID	109729009
Full Name	pumilio homolog 1-like
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020259613.1	XP_020115202.1	pumilio homolog 1-like
XM_020259612.1	XP_020115201.1	pumilio homolog 1-like

Pathways from BioSystems
Gene Ontology

The following LOC109729009 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109729009 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn84921	XM_020259613.1 Latest version!	Ananas comosus pumilio homolog 1-like (LOC109729009), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OAn84921	XM_020259612.1 Latest version!	Ananas comosus pumilio homolog 1-like (LOC109729009), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109729009 ( XM_020259613.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020115202.1 Ananas comosus pumilio homolog 1-like (LOC109729009), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAn84921
Clone ID Related Accession (Same CDS sequence)	XM_020259613.1 , XM_020259612.1
Accession Version	XM_020259613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	pumilio homolog 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTG CGACTGTAAG GAATGCGAAT TTTGAGGACT TAGAGAGGGA TCTCGAAGCC 
CTGCTCCGGG ACCAGAACCC AGGTAGGGGA GCCACCTCCA CCGCCGCCGC CGCCGCCGCC 
GCCGCCTTTG ATCTCGATCG AGATGAGCTC AATATCTACC GGAGTGGAAG CGCTCCTCCA 
ACTGTCGAGG GATCGAGAAC CGCGTTTGGG AGCTTGTTCG GGCACCCCGG ATTCGCCGAA 
ACCCAGGATT TCAGTGGCCC CGATGTTTCC GACGTGCTCT CTGAGGAGGA GATGCGATCG 
CACCCGGCCT ACTTGTCCTA TTATTACTCG AACGAGCATG TGAATCCGCG GCTGCCGCCT 
CCGAGGGTGT CGAAGGAGGA TTGGCGTGCC GCGCAGCGGT TTCATGCCCT GGGGGGTACC 
GGCGATCGGA AGAAGATGAT GGAGATGGGG AGCGCCGATG GGAGTAGCAG CAGCTCTCTC 
TTCTCGTTGC AGCCTGGGTT GCCCTTGCGC GATGGCGAGA GGGAGATGAC CGAGACGGAT 
AGAGGGGCGT TCAAGAATCT CTCTCGGCGG CAGCAGCAGC AGCAGCAGCT GCGGCAGCCA 
GCTGATTGGC TCGAAAAGGG CTCTGATGGG CTTGTTGGTT TGCCTGATGT TGGGCTCGGA 
TCGAGGAGGA GAAGTTTCGC AGATGCATTG CAGGAAGAAC TTGGGCATTT TTCTTTATCT 
AGCCAGCTTT CTCGCCCTGC TAGTCGCAGT GCCTTCGAGA GTCCCAATCC TATAAGAGTT 
TCTGATTTGC CAATGGGACA ACTCCATAGC GGTTCAGATG CCTTAAATGT CTTGCAGTCG 
GGGGCGAGCT CCTCTAGTCT TGCTAGAGTT AAGAGCCTTG GTTCATCAAT TTCTCACTCC 
TATGCGTCGG CTTTAGGCTC TTCTCTTTCT AGGAGTACGA CCCCGGATCC TCTATTGATC 
CGTAGGTCGC CGAGCCCCTG CCTTCCTCCT GTGGGGGTTA GAATTACTGA TAGCGAGAAA 
AAGGCTCCTG TAGTGCCAAA TGAGGGTGGT GGTGCTTCTT CGATTATTTC TAATTCTGCC 
GACATAACAG CTGCCATATC AGCTATATCT AACTTAGGCT TAGCAGAGAA TAGGATCGAA 
GATAGAGAAA GTCAAGCGCA GAGCCAACTT CATCATGAGT TTTCTGATCA ACCCGATATG 
ATATTCAATG TGCCTAGTGA TAGACAAAAA ACCGTTACCT ACAATGAATT TTCCAAGAAT 
AATGGTAATT TAACTAATTT ACCTAAGTTA ACTTCTAATG GCAAAGTCAA CTTTGCTGCA 
CAGTCCGCGT ATGTTAATGT TTATAAAGAA GGAGCTTCTA TGGGCTCGGT GAATTCAGCA 
GGTTCTAATA GTTCTCAGGG ACAAAATGCT GATAACATAA ATATTGGCTT CTCCCGACGC 
AATTCAAAGG CTTATTCTGG TGATTATGGA TCACCATTAT TGAGCAATCA GCTTAATGCA 
GGTGGGCTAG AAGGACAATA TCTTAATTTA TCTGGAAACC AGATGGGATC TGGTTTTCAG 
GTCCCAATGG TGGACCCTCT ATATGCTCAA TATTTGCAAA CGACTCCTGA TTCTATATTA 
AATTCGACTG CTGCAAGCAT GGATCCTTCT TTGGGGAGGA ATTATATTGG CTCCTCACAT 
ATGGATCTCC CTGATTACCA AAAAGCTTAT CTTGGGGCAT TACTCGCACA ACAGAAGTTA 
CAGTATGGCA TGCCCTTCCT GAACAAATCT GTGGGTTTAA ATCATGCCTT CTATGGGAGT 
AACCATGCTT TAGGTGTTGG AATGGCTTAT CCAACTAACC AAGCATCTAG TTCTTTTCTT 
CCCTCATTGG GTTCTGGGAG CCCAGTCCGG CAAGCAAGCG AGCGGCTTAT GCGCGTCTCT 
TCCATGACCA GAGGTGCCGG TGGATCCATA GGATCGCGAA GCCCTGAAAA TGGCTTTATG 
GAAGAAGGTT ATGCATCATC TTTATTGGAA GAATTCAAGA ATAATAAGAC CAGATCTTTT 
GAACTGCAAG ATATTGTTGA TCATGTAGTT GAGTTTAGTG CGGATCAGTA TGGTAGTCGC 
TTTATACAGC AGAAGCTTGA AACTGCAACA GTTGATGAGA AAAACAAAAT CTTTCCTGAG 
ATACTTCCAA AAGCTCGTGC TTTGATGACT GATGTTTTTG GAAATTATGT CATACAGAAG 
TTCTTTGAGC ATGGTACAGA AACTCAGAGA AAGCAATTGG CAAGTGAACT TACAGGCCAT 
GTTATGCAGC TTAGTCTTCA GATGTATGGC TGTAGAGTGA TTCAGAAGGC TTTGGAAGTT 
GTTGATATAG ACCAGAAGAC TCAAATGGTG TTAGAGCTCG ATGGATCAAT CATGAAATGT 
GTCCGTGATC AGAACGGCAA TCATGTGATC CAGAAGTGCA TCGAGTGTGT CCCTCAAGAA 
AGAATCCAGT TTATAATATC AGCATTTTAT GGCCATGTTG TGGCACTTTC CACCCATCCA 
TATGGTTGTC GAGTCATTCA GAGGGTTCTG GAGCACTGTG ACAATCCAAA GACTCAGAGC 
ATTATGATGG ATGAGATCAT GCAATCTGTT TGCACTTTGG CACAAGACCA ATATGGAAAT 
TATGTTATTC AACATGTCCT GCAACAAGGT AAGCCGGAAG AACGGTCTGC TATTATTAGC 
AAGCTTGCTG GACAGATAGT GAAGATGAGC CAACAGAAAT TTGCTTCCAA TGTTGTCGAG 
AAGTGCTTGA CTTATGGTAC TCCCGAGGAA CGCCAGCTCC TAATCAATGA AATGCTTGGT 
TCAACTGATG AAAATGAACC ATTGCAGGTG ATGATGAAAG ATCAATTTGG TAACTATGTC 
GTCCAAAAGG TCCTTGAGAC CTGTGATGAC CATAATCGCG AGCTGATACT TTCACGCATC 
AAGGTGCACT TGAATGCCCT AAAAAGGTAT ACATATGGAA AACACATCGT GGCTCGTGTG 
GAGAAGCTCA TTACTGCCGG AGAAAGGCGC ATTGCACTCG GATCGCATTC TTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020115202.1
CDS	379..3435
Translation	MAPATVRNANFEDLERDLEALLRDQNPGRGATSTAAAAAAAAFDLDRDELNIYRSGSAPPTVEGSRTAFGSLFGHPGFAETQDFSGPDVSDVLSEEEMRSHPAYLSYYYSNEHVNPRLPPPRVSKEDWRAAQRFHALGGTGDRKKMMEMGSADGSSSSSLFSLQPGLPLRDGEREMTETDRGAFKNLSRRQQQQQQLRQPADWLEKGSDGLVGLPDVGLGSRRRSFADALQEELGHFSLSSQLSRPASRSAFESPNPIRVSDLPMGQLHSGSDALNVLQSGASSSSLARVKSLGSSISHSYASALGSSLSRSTTPDPLLIRRSPSPCLPPVGVRITDSEKKAPVVPNEGGGASSIISNSADITAAISAISNLGLAENRIEDRESQAQSQLHHEFSDQPDMIFNVPSDRQKTVTYNEFSKNNGNLTNLPKLTSNGKVNFAAQSAYVNVYKEGASMGSVNSAGSNSSQGQNADNINIGFSRRNSKAYSGDYGSPLLSNQLNAGGLEGQYLNLSGNQMGSGFQVPMVDPLYAQYLQTTPDSILNSTAASMDPSLGRNYIGSSHMDLPDYQKAYLGALLAQQKLQYGMPFLNKSVGLNHAFYGSNHALGVGMAYPTNQASSSFLPSLGSGSPVRQASERLMRVSSMTRGAGGSIGSRSPENGFMEEGYASSLLEEFKNNKTRSFELQDIVDHVVEFSADQYGSRFIQQKLETATVDEKNKIFPEILPKARALMTDVFGNYVIQKFFEHGTETQRKQLASELTGHVMQLSLQMYGCRVIQKALEVVDIDQKTQMVLELDGSIMKCVRDQNGNHVIQKCIECVPQERIQFIISAFYGHVVALSTHPYGCRVIQRVLEHCDNPKTQSIMMDEIMQSVCTLAQDQYGNYVIQHVLQQGKPEERSAIISKLAGQIVKMSQQKFASNVVEKCLTYGTPEERQLLINEMLGSTDENEPLQVMMKDQFGNYVVQKVLETCDDHNRELILSRIKVHLNALKRYTYGKHIVARVEKLITAGERRIALGSHSS

Target ORF information:

RefSeq Version	XM_020259613.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus pumilio homolog 1-like (LOC109729009), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020259613.1

ORF Insert Sequence:

ATGGCGCCTG CGACTGTAAG GAATGCGAAT TTTGAGGACT TAGAGAGGGA TCTCGAAGCC 
CTGCTCCGGG ACCAGAACCC AGGTAGGGGA GCCACCTCCA CCGCCGCCGC CGCCGCCGCC 
GCCGCCTTTG ATCTCGATCG AGATGAGCTC AATATCTACC GGAGTGGAAG CGCTCCTCCA 
ACTGTCGAGG GATCGAGAAC CGCGTTTGGG AGCTTGTTCG GGCACCCCGG ATTCGCCGAA 
ACCCAGGATT TCAGTGGCCC CGATGTTTCC GACGTGCTCT CTGAGGAGGA GATGCGATCG 
CACCCGGCCT ACTTGTCCTA TTATTACTCG AACGAGCATG TGAATCCGCG GCTGCCGCCT 
CCGAGGGTGT CGAAGGAGGA TTGGCGTGCC GCGCAGCGGT TTCATGCCCT GGGGGGTACC 
GGCGATCGGA AGAAGATGAT GGAGATGGGG AGCGCCGATG GGAGTAGCAG CAGCTCTCTC 
TTCTCGTTGC AGCCTGGGTT GCCCTTGCGC GATGGCGAGA GGGAGATGAC CGAGACGGAT 
AGAGGGGCGT TCAAGAATCT CTCTCGGCGG CAGCAGCAGC AGCAGCAGCT GCGGCAGCCA 
GCTGATTGGC TCGAAAAGGG CTCTGATGGG CTTGTTGGTT TGCCTGATGT TGGGCTCGGA 
TCGAGGAGGA GAAGTTTCGC AGATGCATTG CAGGAAGAAC TTGGGCATTT TTCTTTATCT 
AGCCAGCTTT CTCGCCCTGC TAGTCGCAGT GCCTTCGAGA GTCCCAATCC TATAAGAGTT 
TCTGATTTGC CAATGGGACA ACTCCATAGC GGTTCAGATG CCTTAAATGT CTTGCAGTCG 
GGGGCGAGCT CCTCTAGTCT TGCTAGAGTT AAGAGCCTTG GTTCATCAAT TTCTCACTCC 
TATGCGTCGG CTTTAGGCTC TTCTCTTTCT AGGAGTACGA CCCCGGATCC TCTATTGATC 
CGTAGGTCGC CGAGCCCCTG CCTTCCTCCT GTGGGGGTTA GAATTACTGA TAGCGAGAAA 
AAGGCTCCTG TAGTGCCAAA TGAGGGTGGT GGTGCTTCTT CGATTATTTC TAATTCTGCC 
GACATAACAG CTGCCATATC AGCTATATCT AACTTAGGCT TAGCAGAGAA TAGGATCGAA 
GATAGAGAAA GTCAAGCGCA GAGCCAACTT CATCATGAGT TTTCTGATCA ACCCGATATG 
ATATTCAATG TGCCTAGTGA TAGACAAAAA ACCGTTACCT ACAATGAATT TTCCAAGAAT 
AATGGTAATT TAACTAATTT ACCTAAGTTA ACTTCTAATG GCAAAGTCAA CTTTGCTGCA 
CAGTCCGCGT ATGTTAATGT TTATAAAGAA GGAGCTTCTA TGGGCTCGGT GAATTCAGCA 
GGTTCTAATA GTTCTCAGGG ACAAAATGCT GATAACATAA ATATTGGCTT CTCCCGACGC 
AATTCAAAGG CTTATTCTGG TGATTATGGA TCACCATTAT TGAGCAATCA GCTTAATGCA 
GGTGGGCTAG AAGGACAATA TCTTAATTTA TCTGGAAACC AGATGGGATC TGGTTTTCAG 
GTCCCAATGG TGGACCCTCT ATATGCTCAA TATTTGCAAA CGACTCCTGA TTCTATATTA 
AATTCGACTG CTGCAAGCAT GGATCCTTCT TTGGGGAGGA ATTATATTGG CTCCTCACAT 
ATGGATCTCC CTGATTACCA AAAAGCTTAT CTTGGGGCAT TACTCGCACA ACAGAAGTTA 
CAGTATGGCA TGCCCTTCCT GAACAAATCT GTGGGTTTAA ATCATGCCTT CTATGGGAGT 
AACCATGCTT TAGGTGTTGG AATGGCTTAT CCAACTAACC AAGCATCTAG TTCTTTTCTT 
CCCTCATTGG GTTCTGGGAG CCCAGTCCGG CAAGCAAGCG AGCGGCTTAT GCGCGTCTCT 
TCCATGACCA GAGGTGCCGG TGGATCCATA GGATCGCGAA GCCCTGAAAA TGGCTTTATG 
GAAGAAGGTT ATGCATCATC TTTATTGGAA GAATTCAAGA ATAATAAGAC CAGATCTTTT 
GAACTGCAAG ATATTGTTGA TCATGTAGTT GAGTTTAGTG CGGATCAGTA TGGTAGTCGC 
TTTATACAGC AGAAGCTTGA AACTGCAACA GTTGATGAGA AAAACAAAAT CTTTCCTGAG 
ATACTTCCAA AAGCTCGTGC TTTGATGACT GATGTTTTTG GAAATTATGT CATACAGAAG 
TTCTTTGAGC ATGGTACAGA AACTCAGAGA AAGCAATTGG CAAGTGAACT TACAGGCCAT 
GTTATGCAGC TTAGTCTTCA GATGTATGGC TGTAGAGTGA TTCAGAAGGC TTTGGAAGTT 
GTTGATATAG ACCAGAAGAC TCAAATGGTG TTAGAGCTCG ATGGATCAAT CATGAAATGT 
GTCCGTGATC AGAACGGCAA TCATGTGATC CAGAAGTGCA TCGAGTGTGT CCCTCAAGAA 
AGAATCCAGT TTATAATATC AGCATTTTAT GGCCATGTTG TGGCACTTTC CACCCATCCA 
TATGGTTGTC GAGTCATTCA GAGGGTTCTG GAGCACTGTG ACAATCCAAA GACTCAGAGC 
ATTATGATGG ATGAGATCAT GCAATCTGTT TGCACTTTGG CACAAGACCA ATATGGAAAT 
TATGTTATTC AACATGTCCT GCAACAAGGT AAGCCGGAAG AACGGTCTGC TATTATTAGC 
AAGCTTGCTG GACAGATAGT GAAGATGAGC CAACAGAAAT TTGCTTCCAA TGTTGTCGAG 
AAGTGCTTGA CTTATGGTAC TCCCGAGGAA CGCCAGCTCC TAATCAATGA AATGCTTGGT 
TCAACTGATG AAAATGAACC ATTGCAGGTG ATGATGAAAG ATCAATTTGG TAACTATGTC 
GTCCAAAAGG TCCTTGAGAC CTGTGATGAC CATAATCGCG AGCTGATACT TTCACGCATC 
AAGGTGCACT TGAATGCCCT AAAAAGGTAT ACATATGGAA AACACATCGT GGCTCGTGTG 
GAGAAGCTCA TTACTGCCGG AGAAAGGCGC ATTGCACTCG GATCGCATTC TTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC109729009 ( XM_020259612.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020115201.1 Ananas comosus pumilio homolog 1-like (LOC109729009), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAn84921
Clone ID Related Accession (Same CDS sequence)	XM_020259613.1 , XM_020259612.1
Accession Version	XM_020259612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	pumilio homolog 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033645.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTG CGACTGTAAG GAATGCGAAT TTTGAGGACT TAGAGAGGGA TCTCGAAGCC 
CTGCTCCGGG ACCAGAACCC AGGTAGGGGA GCCACCTCCA CCGCCGCCGC CGCCGCCGCC 
GCCGCCTTTG ATCTCGATCG AGATGAGCTC AATATCTACC GGAGTGGAAG CGCTCCTCCA 
ACTGTCGAGG GATCGAGAAC CGCGTTTGGG AGCTTGTTCG GGCACCCCGG ATTCGCCGAA 
ACCCAGGATT TCAGTGGCCC CGATGTTTCC GACGTGCTCT CTGAGGAGGA GATGCGATCG 
CACCCGGCCT ACTTGTCCTA TTATTACTCG AACGAGCATG TGAATCCGCG GCTGCCGCCT 
CCGAGGGTGT CGAAGGAGGA TTGGCGTGCC GCGCAGCGGT TTCATGCCCT GGGGGGTACC 
GGCGATCGGA AGAAGATGAT GGAGATGGGG AGCGCCGATG GGAGTAGCAG CAGCTCTCTC 
TTCTCGTTGC AGCCTGGGTT GCCCTTGCGC GATGGCGAGA GGGAGATGAC CGAGACGGAT 
AGAGGGGCGT TCAAGAATCT CTCTCGGCGG CAGCAGCAGC AGCAGCAGCT GCGGCAGCCA 
GCTGATTGGC TCGAAAAGGG CTCTGATGGG CTTGTTGGTT TGCCTGATGT TGGGCTCGGA 
TCGAGGAGGA GAAGTTTCGC AGATGCATTG CAGGAAGAAC TTGGGCATTT TTCTTTATCT 
AGCCAGCTTT CTCGCCCTGC TAGTCGCAGT GCCTTCGAGA GTCCCAATCC TATAAGAGTT 
TCTGATTTGC CAATGGGACA ACTCCATAGC GGTTCAGATG CCTTAAATGT CTTGCAGTCG 
GGGGCGAGCT CCTCTAGTCT TGCTAGAGTT AAGAGCCTTG GTTCATCAAT TTCTCACTCC 
TATGCGTCGG CTTTAGGCTC TTCTCTTTCT AGGAGTACGA CCCCGGATCC TCTATTGATC 
CGTAGGTCGC CGAGCCCCTG CCTTCCTCCT GTGGGGGTTA GAATTACTGA TAGCGAGAAA 
AAGGCTCCTG TAGTGCCAAA TGAGGGTGGT GGTGCTTCTT CGATTATTTC TAATTCTGCC 
GACATAACAG CTGCCATATC AGCTATATCT AACTTAGGCT TAGCAGAGAA TAGGATCGAA 
GATAGAGAAA GTCAAGCGCA GAGCCAACTT CATCATGAGT TTTCTGATCA ACCCGATATG 
ATATTCAATG TGCCTAGTGA TAGACAAAAA ACCGTTACCT ACAATGAATT TTCCAAGAAT 
AATGGTAATT TAACTAATTT ACCTAAGTTA ACTTCTAATG GCAAAGTCAA CTTTGCTGCA 
CAGTCCGCGT ATGTTAATGT TTATAAAGAA GGAGCTTCTA TGGGCTCGGT GAATTCAGCA 
GGTTCTAATA GTTCTCAGGG ACAAAATGCT GATAACATAA ATATTGGCTT CTCCCGACGC 
AATTCAAAGG CTTATTCTGG TGATTATGGA TCACCATTAT TGAGCAATCA GCTTAATGCA 
GGTGGGCTAG AAGGACAATA TCTTAATTTA TCTGGAAACC AGATGGGATC TGGTTTTCAG 
GTCCCAATGG TGGACCCTCT ATATGCTCAA TATTTGCAAA CGACTCCTGA TTCTATATTA 
AATTCGACTG CTGCAAGCAT GGATCCTTCT TTGGGGAGGA ATTATATTGG CTCCTCACAT 
ATGGATCTCC CTGATTACCA AAAAGCTTAT CTTGGGGCAT TACTCGCACA ACAGAAGTTA 
CAGTATGGCA TGCCCTTCCT GAACAAATCT GTGGGTTTAA ATCATGCCTT CTATGGGAGT 
AACCATGCTT TAGGTGTTGG AATGGCTTAT CCAACTAACC AAGCATCTAG TTCTTTTCTT 
CCCTCATTGG GTTCTGGGAG CCCAGTCCGG CAAGCAAGCG AGCGGCTTAT GCGCGTCTCT 
TCCATGACCA GAGGTGCCGG TGGATCCATA GGATCGCGAA GCCCTGAAAA TGGCTTTATG 
GAAGAAGGTT ATGCATCATC TTTATTGGAA GAATTCAAGA ATAATAAGAC CAGATCTTTT 
GAACTGCAAG ATATTGTTGA TCATGTAGTT GAGTTTAGTG CGGATCAGTA TGGTAGTCGC 
TTTATACAGC AGAAGCTTGA AACTGCAACA GTTGATGAGA AAAACAAAAT CTTTCCTGAG 
ATACTTCCAA AAGCTCGTGC TTTGATGACT GATGTTTTTG GAAATTATGT CATACAGAAG 
TTCTTTGAGC ATGGTACAGA AACTCAGAGA AAGCAATTGG CAAGTGAACT TACAGGCCAT 
GTTATGCAGC TTAGTCTTCA GATGTATGGC TGTAGAGTGA TTCAGAAGGC TTTGGAAGTT 
GTTGATATAG ACCAGAAGAC TCAAATGGTG TTAGAGCTCG ATGGATCAAT CATGAAATGT 
GTCCGTGATC AGAACGGCAA TCATGTGATC CAGAAGTGCA TCGAGTGTGT CCCTCAAGAA 
AGAATCCAGT TTATAATATC AGCATTTTAT GGCCATGTTG TGGCACTTTC CACCCATCCA 
TATGGTTGTC GAGTCATTCA GAGGGTTCTG GAGCACTGTG ACAATCCAAA GACTCAGAGC 
ATTATGATGG ATGAGATCAT GCAATCTGTT TGCACTTTGG CACAAGACCA ATATGGAAAT 
TATGTTATTC AACATGTCCT GCAACAAGGT AAGCCGGAAG AACGGTCTGC TATTATTAGC 
AAGCTTGCTG GACAGATAGT GAAGATGAGC CAACAGAAAT TTGCTTCCAA TGTTGTCGAG 
AAGTGCTTGA CTTATGGTAC TCCCGAGGAA CGCCAGCTCC TAATCAATGA AATGCTTGGT 
TCAACTGATG AAAATGAACC ATTGCAGGTG ATGATGAAAG ATCAATTTGG TAACTATGTC 
GTCCAAAAGG TCCTTGAGAC CTGTGATGAC CATAATCGCG AGCTGATACT TTCACGCATC 
AAGGTGCACT TGAATGCCCT AAAAAGGTAT ACATATGGAA AACACATCGT GGCTCGTGTG 
GAGAAGCTCA TTACTGCCGG AGAAAGGCGC ATTGCACTCG GATCGCATTC TTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020115201.1
CDS	357..3413
Translation	MAPATVRNANFEDLERDLEALLRDQNPGRGATSTAAAAAAAAFDLDRDELNIYRSGSAPPTVEGSRTAFGSLFGHPGFAETQDFSGPDVSDVLSEEEMRSHPAYLSYYYSNEHVNPRLPPPRVSKEDWRAAQRFHALGGTGDRKKMMEMGSADGSSSSSLFSLQPGLPLRDGEREMTETDRGAFKNLSRRQQQQQQLRQPADWLEKGSDGLVGLPDVGLGSRRRSFADALQEELGHFSLSSQLSRPASRSAFESPNPIRVSDLPMGQLHSGSDALNVLQSGASSSSLARVKSLGSSISHSYASALGSSLSRSTTPDPLLIRRSPSPCLPPVGVRITDSEKKAPVVPNEGGGASSIISNSADITAAISAISNLGLAENRIEDRESQAQSQLHHEFSDQPDMIFNVPSDRQKTVTYNEFSKNNGNLTNLPKLTSNGKVNFAAQSAYVNVYKEGASMGSVNSAGSNSSQGQNADNINIGFSRRNSKAYSGDYGSPLLSNQLNAGGLEGQYLNLSGNQMGSGFQVPMVDPLYAQYLQTTPDSILNSTAASMDPSLGRNYIGSSHMDLPDYQKAYLGALLAQQKLQYGMPFLNKSVGLNHAFYGSNHALGVGMAYPTNQASSSFLPSLGSGSPVRQASERLMRVSSMTRGAGGSIGSRSPENGFMEEGYASSLLEEFKNNKTRSFELQDIVDHVVEFSADQYGSRFIQQKLETATVDEKNKIFPEILPKARALMTDVFGNYVIQKFFEHGTETQRKQLASELTGHVMQLSLQMYGCRVIQKALEVVDIDQKTQMVLELDGSIMKCVRDQNGNHVIQKCIECVPQERIQFIISAFYGHVVALSTHPYGCRVIQRVLEHCDNPKTQSIMMDEIMQSVCTLAQDQYGNYVIQHVLQQGKPEERSAIISKLAGQIVKMSQQKFASNVVEKCLTYGTPEERQLLINEMLGSTDENEPLQVMMKDQFGNYVVQKVLETCDDHNRELILSRIKVHLNALKRYTYGKHIVARVEKLITAGERRIALGSHSS

Target ORF information:

RefSeq Version	XM_020259612.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus pumilio homolog 1-like (LOC109729009), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020259612.1

ORF Insert Sequence:

ATGGCGCCTG CGACTGTAAG GAATGCGAAT TTTGAGGACT TAGAGAGGGA TCTCGAAGCC 
CTGCTCCGGG ACCAGAACCC AGGTAGGGGA GCCACCTCCA CCGCCGCCGC CGCCGCCGCC 
GCCGCCTTTG ATCTCGATCG AGATGAGCTC AATATCTACC GGAGTGGAAG CGCTCCTCCA 
ACTGTCGAGG GATCGAGAAC CGCGTTTGGG AGCTTGTTCG GGCACCCCGG ATTCGCCGAA 
ACCCAGGATT TCAGTGGCCC CGATGTTTCC GACGTGCTCT CTGAGGAGGA GATGCGATCG 
CACCCGGCCT ACTTGTCCTA TTATTACTCG AACGAGCATG TGAATCCGCG GCTGCCGCCT 
CCGAGGGTGT CGAAGGAGGA TTGGCGTGCC GCGCAGCGGT TTCATGCCCT GGGGGGTACC 
GGCGATCGGA AGAAGATGAT GGAGATGGGG AGCGCCGATG GGAGTAGCAG CAGCTCTCTC 
TTCTCGTTGC AGCCTGGGTT GCCCTTGCGC GATGGCGAGA GGGAGATGAC CGAGACGGAT 
AGAGGGGCGT TCAAGAATCT CTCTCGGCGG CAGCAGCAGC AGCAGCAGCT GCGGCAGCCA 
GCTGATTGGC TCGAAAAGGG CTCTGATGGG CTTGTTGGTT TGCCTGATGT TGGGCTCGGA 
TCGAGGAGGA GAAGTTTCGC AGATGCATTG CAGGAAGAAC TTGGGCATTT TTCTTTATCT 
AGCCAGCTTT CTCGCCCTGC TAGTCGCAGT GCCTTCGAGA GTCCCAATCC TATAAGAGTT 
TCTGATTTGC CAATGGGACA ACTCCATAGC GGTTCAGATG CCTTAAATGT CTTGCAGTCG 
GGGGCGAGCT CCTCTAGTCT TGCTAGAGTT AAGAGCCTTG GTTCATCAAT TTCTCACTCC 
TATGCGTCGG CTTTAGGCTC TTCTCTTTCT AGGAGTACGA CCCCGGATCC TCTATTGATC 
CGTAGGTCGC CGAGCCCCTG CCTTCCTCCT GTGGGGGTTA GAATTACTGA TAGCGAGAAA 
AAGGCTCCTG TAGTGCCAAA TGAGGGTGGT GGTGCTTCTT CGATTATTTC TAATTCTGCC 
GACATAACAG CTGCCATATC AGCTATATCT AACTTAGGCT TAGCAGAGAA TAGGATCGAA 
GATAGAGAAA GTCAAGCGCA GAGCCAACTT CATCATGAGT TTTCTGATCA ACCCGATATG 
ATATTCAATG TGCCTAGTGA TAGACAAAAA ACCGTTACCT ACAATGAATT TTCCAAGAAT 
AATGGTAATT TAACTAATTT ACCTAAGTTA ACTTCTAATG GCAAAGTCAA CTTTGCTGCA 
CAGTCCGCGT ATGTTAATGT TTATAAAGAA GGAGCTTCTA TGGGCTCGGT GAATTCAGCA 
GGTTCTAATA GTTCTCAGGG ACAAAATGCT GATAACATAA ATATTGGCTT CTCCCGACGC 
AATTCAAAGG CTTATTCTGG TGATTATGGA TCACCATTAT TGAGCAATCA GCTTAATGCA 
GGTGGGCTAG AAGGACAATA TCTTAATTTA TCTGGAAACC AGATGGGATC TGGTTTTCAG 
GTCCCAATGG TGGACCCTCT ATATGCTCAA TATTTGCAAA CGACTCCTGA TTCTATATTA 
AATTCGACTG CTGCAAGCAT GGATCCTTCT TTGGGGAGGA ATTATATTGG CTCCTCACAT 
ATGGATCTCC CTGATTACCA AAAAGCTTAT CTTGGGGCAT TACTCGCACA ACAGAAGTTA 
CAGTATGGCA TGCCCTTCCT GAACAAATCT GTGGGTTTAA ATCATGCCTT CTATGGGAGT 
AACCATGCTT TAGGTGTTGG AATGGCTTAT CCAACTAACC AAGCATCTAG TTCTTTTCTT 
CCCTCATTGG GTTCTGGGAG CCCAGTCCGG CAAGCAAGCG AGCGGCTTAT GCGCGTCTCT 
TCCATGACCA GAGGTGCCGG TGGATCCATA GGATCGCGAA GCCCTGAAAA TGGCTTTATG 
GAAGAAGGTT ATGCATCATC TTTATTGGAA GAATTCAAGA ATAATAAGAC CAGATCTTTT 
GAACTGCAAG ATATTGTTGA TCATGTAGTT GAGTTTAGTG CGGATCAGTA TGGTAGTCGC 
TTTATACAGC AGAAGCTTGA AACTGCAACA GTTGATGAGA AAAACAAAAT CTTTCCTGAG 
ATACTTCCAA AAGCTCGTGC TTTGATGACT GATGTTTTTG GAAATTATGT CATACAGAAG 
TTCTTTGAGC ATGGTACAGA AACTCAGAGA AAGCAATTGG CAAGTGAACT TACAGGCCAT 
GTTATGCAGC TTAGTCTTCA GATGTATGGC TGTAGAGTGA TTCAGAAGGC TTTGGAAGTT 
GTTGATATAG ACCAGAAGAC TCAAATGGTG TTAGAGCTCG ATGGATCAAT CATGAAATGT 
GTCCGTGATC AGAACGGCAA TCATGTGATC CAGAAGTGCA TCGAGTGTGT CCCTCAAGAA 
AGAATCCAGT TTATAATATC AGCATTTTAT GGCCATGTTG TGGCACTTTC CACCCATCCA 
TATGGTTGTC GAGTCATTCA GAGGGTTCTG GAGCACTGTG ACAATCCAAA GACTCAGAGC 
ATTATGATGG ATGAGATCAT GCAATCTGTT TGCACTTTGG CACAAGACCA ATATGGAAAT 
TATGTTATTC AACATGTCCT GCAACAAGGT AAGCCGGAAG AACGGTCTGC TATTATTAGC 
AAGCTTGCTG GACAGATAGT GAAGATGAGC CAACAGAAAT TTGCTTCCAA TGTTGTCGAG 
AAGTGCTTGA CTTATGGTAC TCCCGAGGAA CGCCAGCTCC TAATCAATGA AATGCTTGGT 
TCAACTGATG AAAATGAACC ATTGCAGGTG ATGATGAAAG ATCAATTTGG TAACTATGTC 
GTCCAAAAGG TCCTTGAGAC CTGTGATGAC CATAATCGCG AGCTGATACT TTCACGCATC 
AAGGTGCACT TGAATGCCCT AAAAAGGTAT ACATATGGAA AACACATCGT GGCTCGTGTG 
GAGAAGCTCA TTACTGCCGG AGAAAGGCGC ATTGCACTCG GATCGCATTC TTCATGA