LOC109728258 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109728258
Entrez Gene ID	109728258
Full Name	probable inactive serine/threonine-protein kinase bub1
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020258630.1	XP_020114219.1	probable inactive serine/threonine-protein kinase bub1

Pathways from BioSystems
Gene Ontology

The following LOC109728258 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109728258 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn84203	XM_020258630.1 Latest version!	Ananas comosus probable inactive serine/threonine-protein kinase bub1 (LOC109728258), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109728258 ( XM_020258630.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020114219.1 Ananas comosus probable inactive serine/threonine-protein kinase bub1 (LOC109728258), mRNA.

Price & Availability↑

CloneID	OAn84203
Clone ID Related Accession (Same CDS sequence)	XM_020258630.1
Accession Version	XM_020258630.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	probable inactive serine/threonine-protein kinase bub1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033643.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACCG ATCCCCACAA GGAGCTCTTC TCCTCCCTCG TCGCCGACAT CAAGACCTAC 
TCCGGCTCCG ACCCTCTCCG TCCATGGCTT CGGGGTATCC GGAGGTTGAA GGAGGCCCTC 
CCCTCCCGAT CCCTCAAGGA GAAGCTCCCC CGATTCCTCC AGAAGTGCGC GCAAGCCTTT 
GAATCGGACC GCCGCTACCG CGACGATCCG CGCTACCTCC GCGTCTGGAT CGAACTGATG 
GATTATGTGG ATGATGCTAA GGTGCTGTTG AAAAAGATGG AGAAGAATCA GATTGGCACA 
AAGCGAGCTA TCTTTTATTT GGCATCTGCT CTGTACTATG AAAAGCATAA AAAATTTGAG 
AGGGCAGAGA AGATATATCG ACAGGGGGTT GAGAACCTTG CAGAGCCTGT TGGTGAGTTA 
CAGAAATCCT ACGAGCAGTT CCTTCATCGT ATGGAGCTGT GCAAGAAAAG AAAAGCTAAA 
CTGCAGGAAG TGATGCATAA GAAGGGGAAG TTAAATCTGA AAGGGTCAAG AGGAGAGGGG 
ACTTTGAATA ATTCTCTTCC CAACGCTGAT GCTGAGCATC TTAAAAAAGT GGTGGAAAAA 
CCTACGTTGG TAGGAGGATT TATTGGACCT ATTGAGGATG GTAGTAAAGA TTTAATTTCA 
CTGGAAAATA TACCACTTGA AAATTCTGGA AACATTTGTG ACGATTGGCT GGTTAGCAAT 
CCTCGGCATT GTAGCGAGAA AGATTTAGAA TCGACCATTC CTAAAGTTAG ATCACAAAAT 
AAAAGAAATC CTAATATTGA TAAGGAGAAG CTCAGTAGAT TTAACAGTGA CGATACAGTT 
GTTGTAAAGT TTGTAGATTC TGCCATCGTT GGAAAATCAG AAGCAGAAGA TGCCTGTCAT 
CATGGTCTTG TGGATCCAAC CATAAACATG AGGGAGGCCA TGAGTGCAAT TAACAACATG 
TTTCGAGAGC CATTGGAGTT AGATCCAAGC ATAAGCAGAA GATCACAGCG AAATCGTGCA 
AAGTTGAATC GACCAACTAG TGGGTTTGAG ATCTTTGTTG ATGAAGACGG GCCAAATCCT 
ATCAGGGAAA ATCCCAAATA TAGCCAAAGT GAAGCTAAAC ATGAGAAAAG CCAACCCTTC 
AGTACAAATT CCTCGATGAA GCAAACAAAA ATAGAGTTAC AAAAACCATT TGTTGGCATA 
TTTAAGATGC TGGCTGATGA CGAGGAAGAA GAGAATAGTG AAAACGATGA TGAAGGAATT 
GGTAGCTATT TGCAAGGATG TGAGAAGCCA AAAGGTTTAA AGGGTGACAT TGCCAATGAT 
GTAACTATGA TCAATTACGG ACTCAATGAA GACACAGTGG TTCATAGATT TGTTGGATCG 
GCAGTTTTCG GTGAACCCAA AGTGGAGAAT GCATGCCACC ATGGTTTAGT TGATCCCACT 
GTCAATTTGA AGGAGGCTAT GGATGAAATA AACAGCATGT TTGGGAAGCC GCTTAACTTT 
TTCAAGGGCG AGAAACCACG GGGAAAGCAA AGTAACAAGA GAATGGAATT AAAACCAGCT 
TCTTCTGAGT TCTCTATTTT CGTCGATGAA GATTTGGGAG AAAATATAAA TGAGAAACGA 
CAGGGAAAGC AGAGTAACAA GAGAATAGAT TTAAAACCAG CTTCTTCTGT GTTCTCTATT 
TTGAGTGACA AAGATTCAAG AGAAAATTCA AATGGACAAG CTTTTACAAA CACCTCGAAT 
AAATTTGGAG GAGAAGTTGA TCTGTTTGAG CCAACAATCT TTACTAGGGA GGCTATGGCC 
GAGATCAATG ATTTGTTTGG AAAGCCATTG GACTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020114219.1
CDS	75..1913
Translation	MATDPHKELFSSLVADIKTYSGSDPLRPWLRGIRRLKEALPSRSLKEKLPRFLQKCAQAFESDRRYRDDPRYLRVWIELMDYVDDAKVLLKKMEKNQIGTKRAIFYLASALYYEKHKKFERAEKIYRQGVENLAEPVGELQKSYEQFLHRMELCKKRKAKLQEVMHKKGKLNLKGSRGEGTLNNSLPNADAEHLKKVVEKPTLVGGFIGPIEDGSKDLISLENIPLENSGNICDDWLVSNPRHCSEKDLESTIPKVRSQNKRNPNIDKEKLSRFNSDDTVVVKFVDSAIVGKSEAEDACHHGLVDPTINMREAMSAINNMFREPLELDPSISRRSQRNRAKLNRPTSGFEIFVDEDGPNPIRENPKYSQSEAKHEKSQPFSTNSSMKQTKIELQKPFVGIFKMLADDEEEENSENDDEGIGSYLQGCEKPKGLKGDIANDVTMINYGLNEDTVVHRFVGSAVFGEPKVENACHHGLVDPTVNLKEAMDEINSMFGKPLNFFKGEKPRGKQSNKRMELKPASSEFSIFVDEDLGENINEKRQGKQSNKRIDLKPASSVFSILSDKDSRENSNGQAFTNTSNKFGGEVDLFEPTIFTREAMAEINDLFGKPLDF

Target ORF information:

RefSeq Version	XM_020258630.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus probable inactive serine/threonine-protein kinase bub1 (LOC109728258), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020258630.1

ORF Insert Sequence:

ATGGCGACCG ATCCCCACAA GGAGCTCTTC TCCTCCCTCG TCGCCGACAT CAAGACCTAC 
TCCGGCTCCG ACCCTCTCCG TCCATGGCTT CGGGGTATCC GGAGGTTGAA GGAGGCCCTC 
CCCTCCCGAT CCCTCAAGGA GAAGCTCCCC CGATTCCTCC AGAAGTGCGC GCAAGCCTTT 
GAATCGGACC GCCGCTACCG CGACGATCCG CGCTACCTCC GCGTCTGGAT CGAACTGATG 
GATTATGTGG ATGATGCTAA GGTGCTGTTG AAAAAGATGG AGAAGAATCA GATTGGCACA 
AAGCGAGCTA TCTTTTATTT GGCATCTGCT CTGTACTATG AAAAGCATAA AAAATTTGAG 
AGGGCAGAGA AGATATATCG ACAGGGGGTT GAGAACCTTG CAGAGCCTGT TGGTGAGTTA 
CAGAAATCCT ACGAGCAGTT CCTTCATCGT ATGGAGCTGT GCAAGAAAAG AAAAGCTAAA 
CTGCAGGAAG TGATGCATAA GAAGGGGAAG TTAAATCTGA AAGGGTCAAG AGGAGAGGGG 
ACTTTGAATA ATTCTCTTCC CAACGCTGAT GCTGAGCATC TTAAAAAAGT GGTGGAAAAA 
CCTACGTTGG TAGGAGGATT TATTGGACCT ATTGAGGATG GTAGTAAAGA TTTAATTTCA 
CTGGAAAATA TACCACTTGA AAATTCTGGA AACATTTGTG ACGATTGGCT GGTTAGCAAT 
CCTCGGCATT GTAGCGAGAA AGATTTAGAA TCGACCATTC CTAAAGTTAG ATCACAAAAT 
AAAAGAAATC CTAATATTGA TAAGGAGAAG CTCAGTAGAT TTAACAGTGA CGATACAGTT 
GTTGTAAAGT TTGTAGATTC TGCCATCGTT GGAAAATCAG AAGCAGAAGA TGCCTGTCAT 
CATGGTCTTG TGGATCCAAC CATAAACATG AGGGAGGCCA TGAGTGCAAT TAACAACATG 
TTTCGAGAGC CATTGGAGTT AGATCCAAGC ATAAGCAGAA GATCACAGCG AAATCGTGCA 
AAGTTGAATC GACCAACTAG TGGGTTTGAG ATCTTTGTTG ATGAAGACGG GCCAAATCCT 
ATCAGGGAAA ATCCCAAATA TAGCCAAAGT GAAGCTAAAC ATGAGAAAAG CCAACCCTTC 
AGTACAAATT CCTCGATGAA GCAAACAAAA ATAGAGTTAC AAAAACCATT TGTTGGCATA 
TTTAAGATGC TGGCTGATGA CGAGGAAGAA GAGAATAGTG AAAACGATGA TGAAGGAATT 
GGTAGCTATT TGCAAGGATG TGAGAAGCCA AAAGGTTTAA AGGGTGACAT TGCCAATGAT 
GTAACTATGA TCAATTACGG ACTCAATGAA GACACAGTGG TTCATAGATT TGTTGGATCG 
GCAGTTTTCG GTGAACCCAA AGTGGAGAAT GCATGCCACC ATGGTTTAGT TGATCCCACT 
GTCAATTTGA AGGAGGCTAT GGATGAAATA AACAGCATGT TTGGGAAGCC GCTTAACTTT 
TTCAAGGGCG AGAAACCACG GGGAAAGCAA AGTAACAAGA GAATGGAATT AAAACCAGCT 
TCTTCTGAGT TCTCTATTTT CGTCGATGAA GATTTGGGAG AAAATATAAA TGAGAAACGA 
CAGGGAAAGC AGAGTAACAA GAGAATAGAT TTAAAACCAG CTTCTTCTGT GTTCTCTATT 
TTGAGTGACA AAGATTCAAG AGAAAATTCA AATGGACAAG CTTTTACAAA CACCTCGAAT 
AAATTTGGAG GAGAAGTTGA TCTGTTTGAG CCAACAATCT TTACTAGGGA GGCTATGGCC 
GAGATCAATG ATTTGTTTGG AAAGCCATTG GACTTCTAA