LOC109716182 cDNA ORF clone, Ananas comosus(pineapple)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC109716182
Entrez Gene ID	109716182
Full Name	dihydrofolate synthetase
Gene Type	protein-coding
Organism	Ananas comosus(pineapple)

mRNA and Protein(s)

mRNA	Protein	Name
XM_020241511.1	XP_020097100.1	dihydrofolate synthetase

Pathways from BioSystems
Gene Ontology

The following LOC109716182 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC109716182 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAn71448	XM_020241511.1 Latest version!	Ananas comosus dihydrofolate synthetase (LOC109716182), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC109716182 ( XM_020241511.1 ) cDNA ORF clone, Ananas comosus(pineapple) -> XP_020097100.1 Ananas comosus dihydrofolate synthetase (LOC109716182), mRNA.

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CloneID	OAn71448
Clone ID Related Accession (Same CDS sequence)	XM_020241511.1
Accession Version	XM_020241511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-02-14
Organism	Ananas comosus(pineapple)
Product	dihydrofolate synthetase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_033630.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Ananas comosus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCATC TCCCGAAAAT GTCGTACCCA ATTTGCCATT TGGGGAGGAG GGGGAGGAGC 
AGCTGCAGTA GCTTGATCTC GCAGCTGAGT TGGCGGCGAT TGCGATCGAG CTCGGCCATG 
GCGCGGGGGG GAGAAGGGGA GGAGCTCGGG GGGTTCTCGG AATCCATGGA GAGGCTCAGG 
AACTACGAGA GGATCGGCGT GCCCAGAGGC GCAGGGACCG ACTCCGGCGA CGGCTTCGAC 
CTTGGCCGGA TGCGGCGGTT GCTCCGCCGC CTCGGCGATC CCCACGCCCT GTTCAAGGCT 
GTTCATATCG CTGGCACTAA AGGGAAAGGT TCATCTGCAG CATTTCTGTC CAATATTTTA 
CGAGAAGGCG GCTATAAAGT TGGCTGCTAT ACCAGTCCAC ATCTTTTGAC CATTCGGGAA 
CGGATTTCTT TAGGAAGAAA TGGAGATCCT GTTTCAGCTG AATTACTAAG GGATACTTTC 
CATGGAGCGA AGGGAGTTCT TGATCAATCG ATAGAGCTGG AAAATGGAGC TCTGACCCAT 
TTTGAGGTGT TCACAGCTCT TGCTTTTCTT CTGTTTTCTC AAGAGAAAGT TGACATCGCC 
ATTATTGAAG CTGGTCTTGG AGGTGCAAGG GATGCAACCA ATGTAATTCG CAACAGTGAA 
CTTGCTGCCT CTGTATTAAC TTCGATTGGC GAGGAACATT TGGCTGCTCT GGGTGGTTCT 
TTAGAAAGTG TGGCGATGGC AAAGTCAGGA ATAATCAAGC AGGGGCGTCC GGTTATTATT 
GGTGGCCCTC TTGAGGAAAA AATTGAGCGC ATTATTAAGG ATAGGGCATC TGTCATGAAT 
TCTCCGGTCA TATCGGCATG TGATCCTGGA ATACGGAGCA TTTTAAAATG CTTTGCGAGA 
GACGATGACG AACCTCGCCA AACTTGTGAC ATAGTTGTTG ACATTCAAAA AGACATGAAA 
CTGAGTATCA AATTACACGA TTTGAAACTT CACATGCTTG GAGCTCATCA GCTACAAAAT 
GCTGTTACGG CAACATGTAC AGCCCTCTGC CTCAGTAAAC AAGGTTGGAG AATATCGGAT 
GCATCCATTC GAGCTGGCTT GGAAAGGACA AAGTTGCATG GTAGAAACCA ATTTCTGACT 
CAGAAGGAAG CCTCAGCTTT TGGGCTTCAA GAAGTGTCAT TACTTGTTGA TGGAGCTCAT 
ACTGAAGCTT CTGCGAAGGG CCTATCAGAT GTAATAAGAA TGGCGCATCC GGATGGGCCG 
TTGGCTCTTG TGGTGGCAAT GGCGAGCGAT AAAGATCATC TTGCATTTGC TCAACAGCTT 
CTTTCAGGCA GGAGACCTGA CATCGTCCTT TTAACTGAGA CGAGCATAAG TGGAGGAAAA 
TCCCGCACGA GTTCGGCTTC AGCTCTGAAA AATATATGGA TAAAGGCTGC GCAAGACCTC 
AGTATCAATC TCATAGACCT TGGAGCGGTC GAACTTCACA AACTTTCCAA GGATAATAAT 
TGCTCGTTCG TATCAATGAG TGACAACGAA ACTATGTTGA TCGGTTACCA AGTTGCACCG 
GTTAATGATT CGATCAGGGT TTGTTCTCAG TTGCTTCAGT CGAAGGAGCA GGATCAGAAT 
GGTCTCATTG TTGTAACAGG CTCCCTGCAT ATAGTATCGT CGCTGTTAGC GGCTGTATAC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_020097100.1
CDS	17..1702
Translation	MLHLPKMSYPICHLGRRGRSSCSSLISQLSWRRLRSSSAMARGGEGEELGGFSESMERLRNYERIGVPRGAGTDSGDGFDLGRMRRLLRRLGDPHALFKAVHIAGTKGKGSSAAFLSNILREGGYKVGCYTSPHLLTIRERISLGRNGDPVSAELLRDTFHGAKGVLDQSIELENGALTHFEVFTALAFLLFSQEKVDIAIIEAGLGGARDATNVIRNSELAASVLTSIGEEHLAALGGSLESVAMAKSGIIKQGRPVIIGGPLEEKIERIIKDRASVMNSPVISACDPGIRSILKCFARDDDEPRQTCDIVVDIQKDMKLSIKLHDLKLHMLGAHQLQNAVTATCTALCLSKQGWRISDASIRAGLERTKLHGRNQFLTQKEASAFGLQEVSLLVDGAHTEASAKGLSDVIRMAHPDGPLALVVAMASDKDHLAFAQQLLSGRRPDIVLLTETSISGGKSRTSSASALKNIWIKAAQDLSINLIDLGAVELHKLSKDNNCSFVSMSDNETMLIGYQVAPVNDSIRVCSQLLQSKEQDQNGLIVVTGSLHIVSSLLAAVYK

Target ORF information:

RefSeq Version	XM_020241511.1
Organism	Ananas comosus(pineapple)
Definition	Ananas comosus dihydrofolate synthetase (LOC109716182), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_020241511.1

ORF Insert Sequence:

ATGCTGCATC TCCCGAAAAT GTCGTACCCA ATTTGCCATT TGGGGAGGAG GGGGAGGAGC 
AGCTGCAGTA GCTTGATCTC GCAGCTGAGT TGGCGGCGAT TGCGATCGAG CTCGGCCATG 
GCGCGGGGGG GAGAAGGGGA GGAGCTCGGG GGGTTCTCGG AATCCATGGA GAGGCTCAGG 
AACTACGAGA GGATCGGCGT GCCCAGAGGC GCAGGGACCG ACTCCGGCGA CGGCTTCGAC 
CTTGGCCGGA TGCGGCGGTT GCTCCGCCGC CTCGGCGATC CCCACGCCCT GTTCAAGGCT 
GTTCATATCG CTGGCACTAA AGGGAAAGGT TCATCTGCAG CATTTCTGTC CAATATTTTA 
CGAGAAGGCG GCTATAAAGT TGGCTGCTAT ACCAGTCCAC ATCTTTTGAC CATTCGGGAA 
CGGATTTCTT TAGGAAGAAA TGGAGATCCT GTTTCAGCTG AATTACTAAG GGATACTTTC 
CATGGAGCGA AGGGAGTTCT TGATCAATCG ATAGAGCTGG AAAATGGAGC TCTGACCCAT 
TTTGAGGTGT TCACAGCTCT TGCTTTTCTT CTGTTTTCTC AAGAGAAAGT TGACATCGCC 
ATTATTGAAG CTGGTCTTGG AGGTGCAAGG GATGCAACCA ATGTAATTCG CAACAGTGAA 
CTTGCTGCCT CTGTATTAAC TTCGATTGGC GAGGAACATT TGGCTGCTCT GGGTGGTTCT 
TTAGAAAGTG TGGCGATGGC AAAGTCAGGA ATAATCAAGC AGGGGCGTCC GGTTATTATT 
GGTGGCCCTC TTGAGGAAAA AATTGAGCGC ATTATTAAGG ATAGGGCATC TGTCATGAAT 
TCTCCGGTCA TATCGGCATG TGATCCTGGA ATACGGAGCA TTTTAAAATG CTTTGCGAGA 
GACGATGACG AACCTCGCCA AACTTGTGAC ATAGTTGTTG ACATTCAAAA AGACATGAAA 
CTGAGTATCA AATTACACGA TTTGAAACTT CACATGCTTG GAGCTCATCA GCTACAAAAT 
GCTGTTACGG CAACATGTAC AGCCCTCTGC CTCAGTAAAC AAGGTTGGAG AATATCGGAT 
GCATCCATTC GAGCTGGCTT GGAAAGGACA AAGTTGCATG GTAGAAACCA ATTTCTGACT 
CAGAAGGAAG CCTCAGCTTT TGGGCTTCAA GAAGTGTCAT TACTTGTTGA TGGAGCTCAT 
ACTGAAGCTT CTGCGAAGGG CCTATCAGAT GTAATAAGAA TGGCGCATCC GGATGGGCCG 
TTGGCTCTTG TGGTGGCAAT GGCGAGCGAT AAAGATCATC TTGCATTTGC TCAACAGCTT 
CTTTCAGGCA GGAGACCTGA CATCGTCCTT TTAACTGAGA CGAGCATAAG TGGAGGAAAA 
TCCCGCACGA GTTCGGCTTC AGCTCTGAAA AATATATGGA TAAAGGCTGC GCAAGACCTC 
AGTATCAATC TCATAGACCT TGGAGCGGTC GAACTTCACA AACTTTCCAA GGATAATAAT 
TGCTCGTTCG TATCAATGAG TGACAACGAA ACTATGTTGA TCGGTTACCA AGTTGCACCG 
GTTAATGATT CGATCAGGGT TTGTTCTCAG TTGCTTCAGT CGAAGGAGCA GGATCAGAAT 
GGTCTCATTG TTGTAACAGG CTCCCTGCAT ATAGTATCGT CGCTGTTAGC GGCTGTATAC 
AAGTAA