crim1 cDNA ORF clone, Amphiprion ocellaris(clown anemonefish)

Gene
Clones
gRNAs

Summary

Gene Symbol	crim1
Entrez Gene ID	111562540
Full Name	cysteine rich transmembrane BMP regulator 1
Gene Type	protein-coding
Organism	Amphiprion ocellaris(clown anemonefish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_023261127.1	XP_023116895.1	cysteine-rich motor neuron 1 protein isoform X1
XM_023261133.1	XP_023116901.1	cysteine-rich motor neuron 1 protein isoform X2

Pathways from BioSystems
Gene Ontology

The following crim1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crim1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm73755	XM_023261127.1 Latest version!	Amphiprion ocellaris cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAm73756	XM_023261133.1 Latest version!	Amphiprion ocellaris cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

crim1 ( XM_023261127.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023116895.1 Amphiprion ocellaris cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm73755
Clone ID Related Accession (Same CDS sequence)	XM_023261127.1
Accession Version	XM_023261127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2817bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	cysteine-rich motor neuron 1 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019371731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGGCA AATGCTCCGT GGTGTTATTG AACGTCCTCT TTCTGGTGCG GGCGCTCTCG 
GCTCATGAGT GCGCGCCGTG TAACCTCCGG ACTTGTCCCG TGAAATCCCC CCACGGCTGC 
GAGGGCGGCC GGACGGTGGC CAGGGACCCG TGCGGATGCT GCGATCAGTG CACCCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG GCGCACGGCT ATTGCGCACT GGGGCTGACG 
TGCGCGTCTG TCAACAAAAC CGGAGCTGCC ACTATCCCTG AAATTGGAGT TTGTAAAGTA 
CTTCCTGACC ATCCAGACGC AGGTCTTGAG GATGAGCGCT GTCCCCTGAT GACGGGCTGT 
GATAGAGCAG GAGGTCAGTG TGTGTGTGAT GCCCGTCACT CCTGCTTGGG CTCTTTCACC 
TACCCGGACC AAGAAACCTG CATGAAGGCC AAGTCAGATG GTCGGAGGCA CGAGCACCGG 
GACAGACACA GAGAGAAGTA TGTGGGACCG TCCAACCCGG CGTGTCTGTT CTCTGGCTGC 
AATCTGACCG CTGAGGGCTG CGTGTGCGAG TCTCAGAGCT GCCACCACCA CTTCGCCTAC 
ATCAACCGCA GCCAGTGCCA GGAAGCCGCA GAGGAGCTGA GGTGTGCCGG GGTCACGTGT 
CCCGTCCTGC AGGTGCCCTC CTGCCCCAAA GGTTCAGTCC TGACCAAGAG CTACACGCCC 
CCTGCTGGTT GCTGCCCCTC CATACCACCC CGGTGCACTT GTGACCTCCG TGCCTGCCAC 
AAGCCCCAGT GCCCGAGTGG ACAGCACCCT GTTCCACTCA GCCAGGCCAG CGGTCACCCA 
GGAGACTGCT GCGATGTCTT TGAGTGCCAG AAAGTTTCTC CAAAGTGTGT CCACAATGGA 
AAGGATTTCT CTGAGGGAGA GGTGTATCGC ATGGACCCCT GCTGGCTGTG TCAGTGTCGG 
GGAGGAATCT CCTTCTGCTC CAAGGCAGAG TGTGCTGAGC TGGACTGTGA AAACTTCTAC 
ATTCCTGAGG GCGAGTGTTG CCCTGTCTGC ATAGATGTGG AGCTGCTGTC AATGGATAGC 
ACTAAGGCCA GTTGCTGGGT GAATAACAAG CTGCGAGCCC ACGAGGAGCA GTGGAAAGAG 
GACGACTGCA CGTTCTGCCA GTGTGTGGAT GGAGAACCTC ACTGTACAGC CATGGCCTGC 
AAACAGAGTT GCCAGAACCC TGTGAAGATC CCTGGAGAGT GCTGTCCATT CTGTGAAGAG 
CCTTCGTACG AAACCGTTAG CCCCATGCTT TGTCCTCCCC TGGAGAACTG CTCCCTGTCA 
GAAAATGATT GCCCCTTTGG ATTCCATCAA GACAAAAATG GATGTCTTCT CTGCCAGTGT 
CTCAACAATG ACTCCTGCCC CGACCTGGCG AAATACTGCT CCCTGCAATG CCCGATGGGA 
TATGAGAGGG ATGATTTTGG CTGTGAGGTT TGCGAGTGCA GTATCCCCAC GCCCAAGTGC 
CGACCTCTGA CCTGCACTAA GACCTGCCCT TATGGTTATG TGAGGAACAA GCACGGCTGT 
GAGATGTGTC GCTGCGTCAA GTGTCCTCCC TTCACCTGTG ACAAGCACTG CAGCAGCGGC 
TATCGACAGA ACAGGAAAGG CTGCAACATC TGCATGTGTA AAGAAAGCGG CCATGTCCCA 
AGCACCACTG TCCCTGCTCC GATGCCCAGC TACTGCCTCA CCTCCAACGG CCACCGATAT 
GAGGAAGGCG ATGGCTGGCA TGACGGCTGT CGTGACTGTT ACTGCCACTC CGGACGAGAG 
ATGTGTGTGC TCATATCGTG TCCCGTTCCC AGCTGTTCTC ACCCTGTCGT CAAGCCTGAC 
CAGTGTTGTC CGACCTGCGA AGATGAGTCA GGCAGCGGCC AGCCAGAGGG GATGGACATG 
GTGGTGTGCA GAGCCCCTGG AGGGGAGTTT TATGTGGAAG GGGAGACCTG GAACCTGGAC 
GAGTGCACAC GCTGCACCTG CAGGAAGGGA CGCGTCCTGT GCGACACTGA AGTCTGTCCC 
CCGGCTCTCT GCCAGACACC AGTCAGGAAC AAGGACACCT GTTGCCACGT GTGCCTAGAT 
GAGACCCTGA GCCCCTTGCT GCCAGTGAAC AACAGCCAAC AGGAGTACTG CATAACCAGC 
GATGGAGACG TGCTGCTCGC CGGAGACTCC TGGAAAGCCA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGTTTCTCT CAGCGCTGCC CTGCTGCCAA CTGCAGGGTG 
CCCGTTTTGC GCAAGGGCCA GTGCTGCCCA CACTGTCTCG ACGTGACCAC ATCGGTGCCC 
GTCATGGTGT CCACAAACGC TCCCAAGACA ACAGTCACTA CCACAGTGGA GAGTGCCCTG 
TGGATCACTA CTGTCACTGT GCCACCCATC ATTGCTGAAA CAGACGAGCC GGGCTTGGGT 
GAGACTTACC CCAGCCAGAC GGAGATGGCC CTCATTTACC AGTCAGCCGC ATGGATCCTG 
GCAGGACTCC TCCTAGCCAT CATCATCTTT CTGGTGGTGG CGCTGCTCAT CAACAAGAAA 
AAGAAGTGGG TGCAGATGTC CTGCTACAGT GCACCAAAGA AGACTGTGAT CCTCAAGAAG 
CATGTAAACA AGAATTCCGT GGTCTATATG GAGCCCTCCA AGGAGAACAA ATTTCAGAAT 
GTGAAGAGCG ACTGCGGCAT CATCCACTTC TCTCCGGAGA TGAGCTCCCC CTGCACGGAC 
AAGATGACCA TCCCCAGGGC CAAGATGAGC CTGGGCAACG ACTGGACGCC GCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023116895.1
CDS	462..3278
Translation	MFGKCSVVLLNVLFLVRALSAHECAPCNLRTCPVKSPHGCEGGRTVARDPCGCCDQCTRLEWEPCGGQDWAHGYCALGLTCASVNKTGAATIPEIGVCKVLPDHPDAGLEDERCPLMTGCDRAGGQCVCDARHSCLGSFTYPDQETCMKAKSDGRRHEHRDRHREKYVGPSNPACLFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVTCPVLQVPSCPKGSVLTKSYTPPAGCCPSIPPRCTCDLRACHKPQCPSGQHPVPLSQASGHPGDCCDVFECQKVSPKCVHNGKDFSEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGEPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPMLCPPLENCSLSENDCPFGFHQDKNGCLLCQCLNNDSCPDLAKYCSLQCPMGYERDDFGCEVCECSIPTPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSSGYRQNRKGCNICMCKESGHVPSTTVPAPMPSYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLISCPVPSCSHPVVKPDQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPVRNKDTCCHVCLDETLSPLLPVNNSQQEYCITSDGDVLLAGDSWKANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLDVTTSVPVMVSTNAPKTTVTTTVESALWITTVTVPPIIAETDEPGLGETYPSQTEMALIYQSAAWILAGLLLAIIIFLVVALLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNVKSDCGIIHFSPEMSSPCTDKMTIPRAKMSLGNDWTPR

Target ORF information:

RefSeq Version	XM_023261127.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023261127.1

ORF Insert Sequence:

ATGTTCGGCA AATGCTCCGT GGTGTTATTG AACGTCCTCT TTCTGGTGCG GGCGCTCTCG 
GCTCATGAGT GCGCGCCGTG TAACCTCCGG ACTTGTCCCG TGAAATCCCC CCACGGCTGC 
GAGGGCGGCC GGACGGTGGC CAGGGACCCG TGCGGATGCT GCGATCAGTG CACCCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG GCGCACGGCT ATTGCGCACT GGGGCTGACG 
TGCGCGTCTG TCAACAAAAC CGGAGCTGCC ACTATCCCTG AAATTGGAGT TTGTAAAGTA 
CTTCCTGACC ATCCAGACGC AGGTCTTGAG GATGAGCGCT GTCCCCTGAT GACGGGCTGT 
GATAGAGCAG GAGGTCAGTG TGTGTGTGAT GCCCGTCACT CCTGCTTGGG CTCTTTCACC 
TACCCGGACC AAGAAACCTG CATGAAGGCC AAGTCAGATG GTCGGAGGCA CGAGCACCGG 
GACAGACACA GAGAGAAGTA TGTGGGACCG TCCAACCCGG CGTGTCTGTT CTCTGGCTGC 
AATCTGACCG CTGAGGGCTG CGTGTGCGAG TCTCAGAGCT GCCACCACCA CTTCGCCTAC 
ATCAACCGCA GCCAGTGCCA GGAAGCCGCA GAGGAGCTGA GGTGTGCCGG GGTCACGTGT 
CCCGTCCTGC AGGTGCCCTC CTGCCCCAAA GGTTCAGTCC TGACCAAGAG CTACACGCCC 
CCTGCTGGTT GCTGCCCCTC CATACCACCC CGGTGCACTT GTGACCTCCG TGCCTGCCAC 
AAGCCCCAGT GCCCGAGTGG ACAGCACCCT GTTCCACTCA GCCAGGCCAG CGGTCACCCA 
GGAGACTGCT GCGATGTCTT TGAGTGCCAG AAAGTTTCTC CAAAGTGTGT CCACAATGGA 
AAGGATTTCT CTGAGGGAGA GGTGTATCGC ATGGACCCCT GCTGGCTGTG TCAGTGTCGG 
GGAGGAATCT CCTTCTGCTC CAAGGCAGAG TGTGCTGAGC TGGACTGTGA AAACTTCTAC 
ATTCCTGAGG GCGAGTGTTG CCCTGTCTGC ATAGATGTGG AGCTGCTGTC AATGGATAGC 
ACTAAGGCCA GTTGCTGGGT GAATAACAAG CTGCGAGCCC ACGAGGAGCA GTGGAAAGAG 
GACGACTGCA CGTTCTGCCA GTGTGTGGAT GGAGAACCTC ACTGTACAGC CATGGCCTGC 
AAACAGAGTT GCCAGAACCC TGTGAAGATC CCTGGAGAGT GCTGTCCATT CTGTGAAGAG 
CCTTCGTACG AAACCGTTAG CCCCATGCTT TGTCCTCCCC TGGAGAACTG CTCCCTGTCA 
GAAAATGATT GCCCCTTTGG ATTCCATCAA GACAAAAATG GATGTCTTCT CTGCCAGTGT 
CTCAACAATG ACTCCTGCCC CGACCTGGCG AAATACTGCT CCCTGCAATG CCCGATGGGA 
TATGAGAGGG ATGATTTTGG CTGTGAGGTT TGCGAGTGCA GTATCCCCAC GCCCAAGTGC 
CGACCTCTGA CCTGCACTAA GACCTGCCCT TATGGTTATG TGAGGAACAA GCACGGCTGT 
GAGATGTGTC GCTGCGTCAA GTGTCCTCCC TTCACCTGTG ACAAGCACTG CAGCAGCGGC 
TATCGACAGA ACAGGAAAGG CTGCAACATC TGCATGTGTA AAGAAAGCGG CCATGTCCCA 
AGCACCACTG TCCCTGCTCC GATGCCCAGC TACTGCCTCA CCTCCAACGG CCACCGATAT 
GAGGAAGGCG ATGGCTGGCA TGACGGCTGT CGTGACTGTT ACTGCCACTC CGGACGAGAG 
ATGTGTGTGC TCATATCGTG TCCCGTTCCC AGCTGTTCTC ACCCTGTCGT CAAGCCTGAC 
CAGTGTTGTC CGACCTGCGA AGATGAGTCA GGCAGCGGCC AGCCAGAGGG GATGGACATG 
GTGGTGTGCA GAGCCCCTGG AGGGGAGTTT TATGTGGAAG GGGAGACCTG GAACCTGGAC 
GAGTGCACAC GCTGCACCTG CAGGAAGGGA CGCGTCCTGT GCGACACTGA AGTCTGTCCC 
CCGGCTCTCT GCCAGACACC AGTCAGGAAC AAGGACACCT GTTGCCACGT GTGCCTAGAT 
GAGACCCTGA GCCCCTTGCT GCCAGTGAAC AACAGCCAAC AGGAGTACTG CATAACCAGC 
GATGGAGACG TGCTGCTCGC CGGAGACTCC TGGAAAGCCA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGTTTCTCT CAGCGCTGCC CTGCTGCCAA CTGCAGGGTG 
CCCGTTTTGC GCAAGGGCCA GTGCTGCCCA CACTGTCTCG ACGTGACCAC ATCGGTGCCC 
GTCATGGTGT CCACAAACGC TCCCAAGACA ACAGTCACTA CCACAGTGGA GAGTGCCCTG 
TGGATCACTA CTGTCACTGT GCCACCCATC ATTGCTGAAA CAGACGAGCC GGGCTTGGGT 
GAGACTTACC CCAGCCAGAC GGAGATGGCC CTCATTTACC AGTCAGCCGC ATGGATCCTG 
GCAGGACTCC TCCTAGCCAT CATCATCTTT CTGGTGGTGG CGCTGCTCAT CAACAAGAAA 
AAGAAGTGGG TGCAGATGTC CTGCTACAGT GCACCAAAGA AGACTGTGAT CCTCAAGAAG 
CATGTAAACA AGAATTCCGT GGTCTATATG GAGCCCTCCA AGGAGAACAA ATTTCAGAAT 
GTGAAGAGCG ACTGCGGCAT CATCCACTTC TCTCCGGAGA TGAGCTCCCC CTGCACGGAC 
AAGATGACCA TCCCCAGGGC CAAGATGAGC CTGGGCAACG ACTGGACGCC GCGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

crim1 ( XM_023261133.1 ) cDNA ORF clone, Amphiprion ocellaris(clown anemonefish) -> XP_023116901.1 Amphiprion ocellaris cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm73756
Clone ID Related Accession (Same CDS sequence)	XM_023261133.1
Accession Version	XM_023261133.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-12
Organism	Amphiprion ocellaris(clown anemonefish)
Product	cysteine-rich motor neuron 1 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_019371731.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Amphiprion ocellaris Annotation Release 100 Annotation Version :: 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCGGCA AATGCTCCGT GGTGTTATTG AACGTCCTCT TTCTGGTGCG GGCGCTCTCG 
GCTCATGAGT GCGCGCCGTG TAACCTCCGG ACTTGTCCCG TGAAATCCCC CCACGGCTGC 
GAGGGCGGCC GGACGGTGGC CAGGGACCCG TGCGGATGCT GCGATCAGTG CACCCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG GCGCACGGCT ATTGCGCACT GGGGCTGACG 
TGCGCGTCTG TCAACAAAAC CGGAGCTGCC ACTATCCCTG AAATTGGAGT TTGTAAAGTA 
CTTCCTGACC ATCCAGACGC AGGTCTTGAG GATGAGCGCT GTCCCCTGAT GACGGGCTGT 
GATAGAGCAG GAGGTCAGTG TGTGTGTGAT GCCCGTCACT CCTGCTTGGG CTCTTTCACC 
TACCCGGACC AAGAAACCTG CATGAAGGCC AAGTCAGATG GTCGGAGGCA CGAGCACCGG 
GACAGACACA GAGAGAAGTA TGTGGGACCG TCCAACCCGG CGTGTCTGTT CTCTGGCTGC 
AATCTGACCG CTGAGGGCTG CGTGTGCGAG TCTCAGAGCT GCCACCACCA CTTCGCCTAC 
ATCAACCGCA GCCAGTGCCA GGAAGCCGCA GAGGAGCTGA GGTGTGCCGG GGTCACGTGT 
CCCGTCCTGC AGGTGCCCTC CTGCCCCAAA GGTTCAGTCC TGACCAAGAG CTACACGCCC 
CCTGCTGGTT GCTGCCCCTC CATACCACCC CGGTGCACTT GTGACCTCCG TGCCTGCCAC 
AAGCCCCAGT GCCCGAGTGG ACAGCACCCT GTTCCACTCA GCCAGGCCAG CGGTCACCCA 
GGAGACTGCT GCGATGTCTT TGAGTGCCAG AAAGTTTCTC CAAAGTGTGT CCACAATGGA 
AAGGATTTCT CTGAGGGAGA GGTGTATCGC ATGGACCCCT GCTGGCTGTG TCAGTGTCGG 
GGAGGAATCT CCTTCTGCTC CAAGGCAGAG TGTGCTGAGC TGGACTGTGA AAACTTCTAC 
ATTCCTGAGG GCGAGTGTTG CCCTGTCTGC ATAGATGTGG AGCTGCTGTC AATGGATAGC 
ACTAAGGCCA GTTGCTGGGT GAATAACAAG CTGCGAGCCC ACGAGGAGCA GTGGAAAGAG 
GACGACTGCA CGTTCTGCCA GTGTGTGGAT GGAGAACCTC ACTGTACAGC CATGGCCTGC 
AAACAGAGTT GCCAGAACCC TGTGAAGATC CCTGGAGAGT GCTGTCCATT CTGTGAAGAG 
CCTTCGTACG AAACCGTTAG CCCCATGCTT TGTCCTCCCC TGGAGAACTG CTCCCTGTCA 
GAAAATGATT GCCCCTTTGG ATTCCATCAA GACAAAAATG GATGTCTTCT CTGCCAGTGT 
CTCAACAATG ACTCCTGCCC CGACCTGGCG AAATACTGCT CCCTGCAATG CCCGATGGGA 
TATGAGAGGG ATGATTTTGG CTGTGAGGTT TGCGAGTGCA GTATCCCCAC GCCCAAGTGC 
CGACCTCTGA CCTGCACTAA GACCTGCCCT TATGGTTATG TGAGGAACAA GCACGGCTGT 
GAGATGTGTC GCTGCGTCAA GTGTCCTCCC TTCACCTGTG ACAAGCACTG CAGCAGCGGC 
TATCGACAGA ACAGGAAAGG CTGCAACATC TGCATGTGTA AAGAAAGCGG CCATGTCCCA 
AGCACCACTG TCCCTGCTCC GATGCCCAGC TACTGCCTCA CCTCCAACGG CCACCGATAT 
GAGGAAGGCG ATGGCTGGCA TGACGGCTGT CGTGACTGTT ACTGCCACTC CGGACGAGAG 
ATGTGTGTGC TCATATCGTG TCCCGTTCCC AGCTGTTCTC ACCCTGTCGT CAAGCCTGAC 
CAGTGTTGTC CGACCTGCGA AGATGAGTCA GGCAGCGGCC AGCCAGAGGG GATGGACATG 
GTGGTGTGCA GAGCCCCTGG AGGGGAGTTT TATGTGGAAG GGGAGACCTG GAACCTGGAC 
GAGTGCACAC GCTGCACCTG CAGGAAGGGA CGCGTCCTGT GCGACACTGA AGTCTGTCCC 
CCGGCTCTCT GCCAGACACC AGTCAGGAAC AAGGACACCT GTTGCCACGT GTGCCTAGAT 
GAGACCCTGA GCCCCTTGCT GCCAGTGAAC AACAGCCAAC AGGAGTACTG CATAACCAGC 
GATGGAGACG TGCTGCTCGC CGGAGACTCC TGGAAAGCCA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGTTTCTCT CAGCGCTGCC CTGCTGCCAA CTGCAGGGTG 
CCCGTTTTGC GCAAGGGCCA GTGCTGCCCA CACTGTCTCG ACGAGCCGGG CTTGGGTGAG 
ACTTACCCCA GCCAGACGGA GATGGCCCTC ATTTACCAGT CAGCCGCATG GATCCTGGCA 
GGACTCCTCC TAGCCATCAT CATCTTTCTG GTGGTGGCGC TGCTCATCAA CAAGAAAAAG 
AAGTGGGTGC AGATGTCCTG CTACAGTGCA CCAAAGAAGA CTGTGATCCT CAAGAAGCAT 
GTAAACAAGA ATTCCGTGGT CTATATGGAG CCCTCCAAGG AGAACAAATT TCAGAATGTG 
AAGAGCGACT GCGGCATCAT CCACTTCTCT CCGGAGATGA GCTCCCCCTG CACGGACAAG 
ATGACCATCC CCAGGGCCAA GATGAGCCTG GGCAACGACT GGACGCCGCG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_023116901.1
CDS	462..3155
Translation	MFGKCSVVLLNVLFLVRALSAHECAPCNLRTCPVKSPHGCEGGRTVARDPCGCCDQCTRLEWEPCGGQDWAHGYCALGLTCASVNKTGAATIPEIGVCKVLPDHPDAGLEDERCPLMTGCDRAGGQCVCDARHSCLGSFTYPDQETCMKAKSDGRRHEHRDRHREKYVGPSNPACLFSGCNLTAEGCVCESQSCHHHFAYINRSQCQEAAEELRCAGVTCPVLQVPSCPKGSVLTKSYTPPAGCCPSIPPRCTCDLRACHKPQCPSGQHPVPLSQASGHPGDCCDVFECQKVSPKCVHNGKDFSEGEVYRMDPCWLCQCRGGISFCSKAECAELDCENFYIPEGECCPVCIDVELLSMDSTKASCWVNNKLRAHEEQWKEDDCTFCQCVDGEPHCTAMACKQSCQNPVKIPGECCPFCEEPSYETVSPMLCPPLENCSLSENDCPFGFHQDKNGCLLCQCLNNDSCPDLAKYCSLQCPMGYERDDFGCEVCECSIPTPKCRPLTCTKTCPYGYVRNKHGCEMCRCVKCPPFTCDKHCSSGYRQNRKGCNICMCKESGHVPSTTVPAPMPSYCLTSNGHRYEEGDGWHDGCRDCYCHSGREMCVLISCPVPSCSHPVVKPDQCCPTCEDESGSGQPEGMDMVVCRAPGGEFYVEGETWNLDECTRCTCRKGRVLCDTEVCPPALCQTPVRNKDTCCHVCLDETLSPLLPVNNSQQEYCITSDGDVLLAGDSWKANACTSCTCNNGTIQCFSQRCPAANCRVPVLRKGQCCPHCLDEPGLGETYPSQTEMALIYQSAAWILAGLLLAIIIFLVVALLINKKKKWVQMSCYSAPKKTVILKKHVNKNSVVYMEPSKENKFQNVKSDCGIIHFSPEMSSPCTDKMTIPRAKMSLGNDWTPR

Target ORF information:

RefSeq Version	XM_023261133.1
Organism	Amphiprion ocellaris(clown anemonefish)
Definition	Amphiprion ocellaris cysteine rich transmembrane BMP regulator 1 (crim1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_023261133.1

ORF Insert Sequence:

ATGTTCGGCA AATGCTCCGT GGTGTTATTG AACGTCCTCT TTCTGGTGCG GGCGCTCTCG 
GCTCATGAGT GCGCGCCGTG TAACCTCCGG ACTTGTCCCG TGAAATCCCC CCACGGCTGC 
GAGGGCGGCC GGACGGTGGC CAGGGACCCG TGCGGATGCT GCGATCAGTG CACCCGGCTG 
GAGTGGGAGC CCTGCGGCGG CCAGGACTGG GCGCACGGCT ATTGCGCACT GGGGCTGACG 
TGCGCGTCTG TCAACAAAAC CGGAGCTGCC ACTATCCCTG AAATTGGAGT TTGTAAAGTA 
CTTCCTGACC ATCCAGACGC AGGTCTTGAG GATGAGCGCT GTCCCCTGAT GACGGGCTGT 
GATAGAGCAG GAGGTCAGTG TGTGTGTGAT GCCCGTCACT CCTGCTTGGG CTCTTTCACC 
TACCCGGACC AAGAAACCTG CATGAAGGCC AAGTCAGATG GTCGGAGGCA CGAGCACCGG 
GACAGACACA GAGAGAAGTA TGTGGGACCG TCCAACCCGG CGTGTCTGTT CTCTGGCTGC 
AATCTGACCG CTGAGGGCTG CGTGTGCGAG TCTCAGAGCT GCCACCACCA CTTCGCCTAC 
ATCAACCGCA GCCAGTGCCA GGAAGCCGCA GAGGAGCTGA GGTGTGCCGG GGTCACGTGT 
CCCGTCCTGC AGGTGCCCTC CTGCCCCAAA GGTTCAGTCC TGACCAAGAG CTACACGCCC 
CCTGCTGGTT GCTGCCCCTC CATACCACCC CGGTGCACTT GTGACCTCCG TGCCTGCCAC 
AAGCCCCAGT GCCCGAGTGG ACAGCACCCT GTTCCACTCA GCCAGGCCAG CGGTCACCCA 
GGAGACTGCT GCGATGTCTT TGAGTGCCAG AAAGTTTCTC CAAAGTGTGT CCACAATGGA 
AAGGATTTCT CTGAGGGAGA GGTGTATCGC ATGGACCCCT GCTGGCTGTG TCAGTGTCGG 
GGAGGAATCT CCTTCTGCTC CAAGGCAGAG TGTGCTGAGC TGGACTGTGA AAACTTCTAC 
ATTCCTGAGG GCGAGTGTTG CCCTGTCTGC ATAGATGTGG AGCTGCTGTC AATGGATAGC 
ACTAAGGCCA GTTGCTGGGT GAATAACAAG CTGCGAGCCC ACGAGGAGCA GTGGAAAGAG 
GACGACTGCA CGTTCTGCCA GTGTGTGGAT GGAGAACCTC ACTGTACAGC CATGGCCTGC 
AAACAGAGTT GCCAGAACCC TGTGAAGATC CCTGGAGAGT GCTGTCCATT CTGTGAAGAG 
CCTTCGTACG AAACCGTTAG CCCCATGCTT TGTCCTCCCC TGGAGAACTG CTCCCTGTCA 
GAAAATGATT GCCCCTTTGG ATTCCATCAA GACAAAAATG GATGTCTTCT CTGCCAGTGT 
CTCAACAATG ACTCCTGCCC CGACCTGGCG AAATACTGCT CCCTGCAATG CCCGATGGGA 
TATGAGAGGG ATGATTTTGG CTGTGAGGTT TGCGAGTGCA GTATCCCCAC GCCCAAGTGC 
CGACCTCTGA CCTGCACTAA GACCTGCCCT TATGGTTATG TGAGGAACAA GCACGGCTGT 
GAGATGTGTC GCTGCGTCAA GTGTCCTCCC TTCACCTGTG ACAAGCACTG CAGCAGCGGC 
TATCGACAGA ACAGGAAAGG CTGCAACATC TGCATGTGTA AAGAAAGCGG CCATGTCCCA 
AGCACCACTG TCCCTGCTCC GATGCCCAGC TACTGCCTCA CCTCCAACGG CCACCGATAT 
GAGGAAGGCG ATGGCTGGCA TGACGGCTGT CGTGACTGTT ACTGCCACTC CGGACGAGAG 
ATGTGTGTGC TCATATCGTG TCCCGTTCCC AGCTGTTCTC ACCCTGTCGT CAAGCCTGAC 
CAGTGTTGTC CGACCTGCGA AGATGAGTCA GGCAGCGGCC AGCCAGAGGG GATGGACATG 
GTGGTGTGCA GAGCCCCTGG AGGGGAGTTT TATGTGGAAG GGGAGACCTG GAACCTGGAC 
GAGTGCACAC GCTGCACCTG CAGGAAGGGA CGCGTCCTGT GCGACACTGA AGTCTGTCCC 
CCGGCTCTCT GCCAGACACC AGTCAGGAAC AAGGACACCT GTTGCCACGT GTGCCTAGAT 
GAGACCCTGA GCCCCTTGCT GCCAGTGAAC AACAGCCAAC AGGAGTACTG CATAACCAGC 
GATGGAGACG TGCTGCTCGC CGGAGACTCC TGGAAAGCCA ACGCCTGCAC CAGCTGCACC 
TGCAACAACG GCACCATTCA GTGTTTCTCT CAGCGCTGCC CTGCTGCCAA CTGCAGGGTG 
CCCGTTTTGC GCAAGGGCCA GTGCTGCCCA CACTGTCTCG ACGAGCCGGG CTTGGGTGAG 
ACTTACCCCA GCCAGACGGA GATGGCCCTC ATTTACCAGT CAGCCGCATG GATCCTGGCA 
GGACTCCTCC TAGCCATCAT CATCTTTCTG GTGGTGGCGC TGCTCATCAA CAAGAAAAAG 
AAGTGGGTGC AGATGTCCTG CTACAGTGCA CCAAAGAAGA CTGTGATCCT CAAGAAGCAT 
GTAAACAAGA ATTCCGTGGT CTATATGGAG CCCTCCAAGG AGAACAAATT TCAGAATGTG 
AAGAGCGACT GCGGCATCAT CCACTTCTCT CCGGAGATGA GCTCCCCCTG CACGGACAAG 
ATGACCATCC CCAGGGCCAA GATGAGCCTG GGCAACGACT GGACGCCGCG CTAA