LOC100640497 cDNA ORF clone, Amphimedon queenslandica

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100640497
Entrez Gene ID	100640497
Full Name	sphingosine-1-phosphate lyase 1-like
Gene Type	protein-coding
Organism	Amphimedon queenslandica

mRNA and Protein(s)

mRNA	Protein	Name
XM_003387011.3	XP_003387059.1	sphingosine-1-phosphate lyase 1-like

Pathways from BioSystems
Gene Ontology

The following LOC100640497 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100640497 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm18690	XM_003387011.3 Latest version!	Amphimedon queenslandica sphingosine-1-phosphate lyase 1-like (LOC100640497), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100640497 ( XM_003387011.3 ) cDNA ORF clone, Amphimedon queenslandica -> XP_003387059.1 Amphimedon queenslandica sphingosine-1-phosphate lyase 1-like (LOC100640497), mRNA.

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CloneID	OAm18690
Clone ID Related Accession (Same CDS sequence)	XM_003387011.3
Accession Version	XM_003387011.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-17
Organism	Amphimedon queenslandica
Product	sphingosine-1-phosphate lyase 1-like
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003546390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 18, 2017 this sequence version replaced XM_003387011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amphimedon queenslandica Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTGTTCA AACTTCTCTT TCTGGCTGCC ATTACTTTCT TCCTGGCAGT TCTATGGGAG 
AGTCCATCAG CTAGACATCA GTTTAGAGAC GAGTTAGAGA AGGCCAGGGG TCACATAAAC 
CGCTCAGTTA AAGGCTATGA AGGATGGCAG ATCATAGCTG TGACTGCTGG TGCCACACTC 
ATCCTTTATA AACTCTACCT CTTCCTTGTT GGAACTGGAG AGAAGACACT TTGGGAGAGA 
ATCAAGGGAA CCTGTTTTAA AACCGTCCGC TCTCTTCCTT TCATTAACGG GAAGATTGAG 
AAAGAGTTAA ATAAGACACG GAGACTCTTA GAAACTGAAC TCTTGACACC AAAACCAGGA 
GAAACCTTTC ACCTTTCGTT ACCAGAGAAG GGACTTTCAC ACGAAGAAAT AATGAAAGAA 
CTGGATGGAA TGGATAAACT TGAACCATAC GACTGGCGAA AAGGATACAC TTCAGGATGT 
GCTTATAACT GCGACGATAA ATTAACTAAA ATAACTTGTG ATGTATTTCG GAGATACTGC 
TGGACTAACC CACTTCATCC TGACATATTC CCTCAAATCC GTAAGATGGA GGCTGAGATT 
GTTCAATGGA CGGCTAAACT GTTCAACGGA GGCAGCACGT CCTGTGGGTG TGTCACTTCT 
GGAGGAACCG AGAGTATTAT GTTGGCAATG AAAGCTTATC GTGGGGTTGG GTACTCTAAA 
GGTATCCAGT ATCCCGAGAT ATTATGTTCT AGCGCTACGC ATGCCGCGTT CAATAAAGCA 
GCTCATTACC TTCGGATGAA AATAACGATC ATTAAATGTG ATCCACTCTC TCGTCAGGTT 
GACGTTAAGG CCATGGCTCG TGCTATTAAT AGTAACACTG TCGTGTTAGT GGCCAACGCC 
CCCCAATACC CGCACGGGGT CATCGACCCG ATACAGAAGA TAAGTGAACT GGCTCTTAAG 
AATAAGATAG GACTTCACGT GGACTCTTGT CTCGGAGGGT TTCTACTTCC TTTTATGGAA 
AGTGCAGGAT ACCCTTTAGA TCCGTTTGAT TTCAGGGTAC CAGGTGTTAC TAGTATCTCA 
GCAGACACTC ATAAGTATGG TTACACTACT AAAGGTTCTT CGGTCGTCTT GTATCGCAAT 
AAGGAACTCC GCCAGCACCA GTTCTTTGTG GTGACAGATT GGGAGGGAGG GATCTATCCA 
TCAGCATCTG TTGCTGGTAG CAGACCAGGT GGTATTATAG CTGCCACGTG GGCAGCAATG 
ATGTATATGG GTATGGATGG GTATGTTGAA TCCACTCGGT CTATTGTTAA GACAACTCGC 
TGGTTGGCAC AAGAGCTGAA CTCCATCCCC CACCTCTATG TGATGGGTAA TCCCATGGTT 
AATGTGGTCG CTATTGCGTC TAAGGATTTC AATATTTACG AGTTAAACGA GGAAATGCGT 
AAAAGGAACT GGAGCCTGAA CCCCCTCCAG TTTCCCCCTG CCGTTCACAT CTGTGTCACT 
ATGATACACA CTAGGGATGG TATGGATCTA AAGTTTCTAA ATGACGTCAA GGAGTGTGTC 
TCCCTCATCA TGAAGGATCC TGGTAAGAAG GTCTCTGGAG CAGCTGCTGT TTATGGTACA 
TCACAGGCAA TTCCTGACCG CTCCATAATA TCTGATATCG GGTACCATTA TCTCAACGTT 
AGCCTCACAG CTGCACCTCT TGAGAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003387059.1
CDS	38..1747
Translation	MLFKLLFLAAITFFLAVLWESPSARHQFRDELEKARGHINRSVKGYEGWQIIAVTAGATLILYKLYLFLVGTGEKTLWERIKGTCFKTVRSLPFINGKIEKELNKTRRLLETELLTPKPGETFHLSLPEKGLSHEEIMKELDGMDKLEPYDWRKGYTSGCAYNCDDKLTKITCDVFRRYCWTNPLHPDIFPQIRKMEAEIVQWTAKLFNGGSTSCGCVTSGGTESIMLAMKAYRGVGYSKGIQYPEILCSSATHAAFNKAAHYLRMKITIIKCDPLSRQVDVKAMARAINSNTVVLVANAPQYPHGVIDPIQKISELALKNKIGLHVDSCLGGFLLPFMESAGYPLDPFDFRVPGVTSISADTHKYGYTTKGSSVVLYRNKELRQHQFFVVTDWEGGIYPSASVAGSRPGGIIAATWAAMMYMGMDGYVESTRSIVKTTRWLAQELNSIPHLYVMGNPMVNVVAIASKDFNIYELNEEMRKRNWSLNPLQFPPAVHICVTMIHTRDGMDLKFLNDVKECVSLIMKDPGKKVSGAAAVYGTSQAIPDRSIISDIGYHYLNVSLTAAPLEN

Target ORF information:

RefSeq Version	XM_003387011.3
Organism	Amphimedon queenslandica
Definition	Amphimedon queenslandica sphingosine-1-phosphate lyase 1-like (LOC100640497), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003387011.3

ORF Insert Sequence:

ATGTTGTTCA AACTTCTCTT TCTGGCTGCC ATTACTTTCT TCCTGGCAGT TCTATGGGAG 
AGTCCATCAG CTAGACATCA GTTTAGAGAC GAGTTAGAGA AGGCCAGGGG TCACATAAAC 
CGCTCAGTTA AAGGCTATGA AGGATGGCAG ATCATAGCTG TGACTGCTGG TGCCACACTC 
ATCCTTTATA AACTCTACCT CTTCCTTGTT GGAACTGGAG AGAAGACACT TTGGGAGAGA 
ATCAAGGGAA CCTGTTTTAA AACCGTCCGC TCTCTTCCTT TCATTAACGG GAAGATTGAG 
AAAGAGTTAA ATAAGACACG GAGACTCTTA GAAACTGAAC TCTTGACACC AAAACCAGGA 
GAAACCTTTC ACCTTTCGTT ACCAGAGAAG GGACTTTCAC ACGAAGAAAT AATGAAAGAA 
CTGGATGGAA TGGATAAACT TGAACCATAC GACTGGCGAA AAGGATACAC TTCAGGATGT 
GCTTATAACT GCGACGATAA ATTAACTAAA ATAACTTGTG ATGTATTTCG GAGATACTGC 
TGGACTAACC CACTTCATCC TGACATATTC CCTCAAATCC GTAAGATGGA GGCTGAGATT 
GTTCAATGGA CGGCTAAACT GTTCAACGGA GGCAGCACGT CCTGTGGGTG TGTCACTTCT 
GGAGGAACCG AGAGTATTAT GTTGGCAATG AAAGCTTATC GTGGGGTTGG GTACTCTAAA 
GGTATCCAGT ATCCCGAGAT ATTATGTTCT AGCGCTACGC ATGCCGCGTT CAATAAAGCA 
GCTCATTACC TTCGGATGAA AATAACGATC ATTAAATGTG ATCCACTCTC TCGTCAGGTT 
GACGTTAAGG CCATGGCTCG TGCTATTAAT AGTAACACTG TCGTGTTAGT GGCCAACGCC 
CCCCAATACC CGCACGGGGT CATCGACCCG ATACAGAAGA TAAGTGAACT GGCTCTTAAG 
AATAAGATAG GACTTCACGT GGACTCTTGT CTCGGAGGGT TTCTACTTCC TTTTATGGAA 
AGTGCAGGAT ACCCTTTAGA TCCGTTTGAT TTCAGGGTAC CAGGTGTTAC TAGTATCTCA 
GCAGACACTC ATAAGTATGG TTACACTACT AAAGGTTCTT CGGTCGTCTT GTATCGCAAT 
AAGGAACTCC GCCAGCACCA GTTCTTTGTG GTGACAGATT GGGAGGGAGG GATCTATCCA 
TCAGCATCTG TTGCTGGTAG CAGACCAGGT GGTATTATAG CTGCCACGTG GGCAGCAATG 
ATGTATATGG GTATGGATGG GTATGTTGAA TCCACTCGGT CTATTGTTAA GACAACTCGC 
TGGTTGGCAC AAGAGCTGAA CTCCATCCCC CACCTCTATG TGATGGGTAA TCCCATGGTT 
AATGTGGTCG CTATTGCGTC TAAGGATTTC AATATTTACG AGTTAAACGA GGAAATGCGT 
AAAAGGAACT GGAGCCTGAA CCCCCTCCAG TTTCCCCCTG CCGTTCACAT CTGTGTCACT 
ATGATACACA CTAGGGATGG TATGGATCTA AAGTTTCTAA ATGACGTCAA GGAGTGTGTC 
TCCCTCATCA TGAAGGATCC TGGTAAGAAG GTCTCTGGAG CAGCTGCTGT TTATGGTACA 
TCACAGGCAA TTCCTGACCG CTCCATAATA TCTGATATCG GGTACCATTA TCTCAACGTT 
AGCCTCACAG CTGCACCTCT TGAGAATTAA