LOC100633849 cDNA ORF clone, Amphimedon queenslandica

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100633849
Entrez Gene ID	100633849
Full Name	glycine dehydrogenase (decarboxylating), mitochondrial-like
Gene Type	protein-coding
Organism	Amphimedon queenslandica

mRNA and Protein(s)

mRNA	Protein	Name
XM_019996081.1	XP_019851640.1	glycine dehydrogenase (decarboxylating), mitochondrial-like isoform X2
XM_019996080.1	XP_019851639.1	glycine dehydrogenase (decarboxylating), mitochondrial-like isoform X1

Pathways from BioSystems
Gene Ontology

The following LOC100633849 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100633849 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OAm17177	XM_019996080.1 Latest version!	Amphimedon queenslandica glycine dehydrogenase (decarboxylating), mitochondrial-like (LOC100633849), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OAm17178	XM_019996081.1 Latest version!	Amphimedon queenslandica glycine dehydrogenase (decarboxylating), mitochondrial-like (LOC100633849), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100633849 ( XM_019996080.1 ) cDNA ORF clone, Amphimedon queenslandica -> XP_019851639.1 Amphimedon queenslandica glycine dehydrogenase (decarboxylating), mitochondrial-like (LOC100633849), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OAm17177
Clone ID Related Accession (Same CDS sequence)	XM_019996080.1
Accession Version	XM_019996080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-17
Organism	Amphimedon queenslandica
Product	glycine dehydrogenase (decarboxylating), mitochondrial-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003546341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amphimedon queenslandica Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAGG TGTATCGGTC TTATCTTGGT CAGGGGTACT ACGGATGTAT AGTACCTCCT 
GTTATCAAAA GAAACGTAAT AGAGAACCCA GGCTGGATAA CACAGTATAC TCCCTATCAA 
GCCGAGCTGG CTCAGGGTAG ACTGGAATCT CTCATGAACT ACCAGACAAT GATAGTCGAC 
CTAACTGGAC TGGATATTGC TAATGCTTCT CTTCTTGACG AAGGAACTGC AGCAGCTGAA 
GCTGTGACAC TCTCTTATAG AATGTTTAGA GGAAAAAGAA ACAAGTACTT CATTGATGAG 
GGGATCCACT CAAACTCGCT TGCGGTCATC AAAACGAGAG CAAAGTTCTT AAATATCGAC 
ATAGTGACTG GTTCGTATCA GGACTTTGTT TTAACTGATG ATGTGTGTGG AGTGATGCTC 
CAGTATCCTA ATAATGATGG TATCATTAAT AATTATCATG ACATCATACA GCAAGCTAAA 
GGATTAAAGA TCCCGGTTGC AATGGCTACT GATCTCCTAG CCCTCACTAT CCTCAAGTCT 
CCTGGTGAAT TAGGTGCCAG TATTGCCTTT GGTAACAGTC AGAGGTTCGG GGTACCAATG 
GGTTGTGGTG GTCCTCATGC TGCATTCTTT GCTGTTAAAG ACGAACTAAA GAAACAAATG 
CCTGGAAGAC TAGTGGGGAA GAGCTGGGAT CAGTATGGTG TCCCTACCTA CAGACTAGCA 
CTACAGACAA GAGAACAGTT CATAAGGAGA GAGAAGGCCA CCAGTAACAT ATGCACTGCC 
CAAGCTTTGC TGGCTAATGT ATCAGCTATG TATGCAGTGT ATCATGGACC CAAAGGACTG 
AAGGATATTG CAATGGAAGT ACATAAAGCA ACTGTACTAT TAGCTGAAGG CCTGAGACTG 
TTAGGTTGGA CTATAAATAA TGAAACTGTC TTTGATACAA TAATGATATC AAATGGAGAC 
ATTAGCACTG ATGAGATACA GAGGAGGTGT GACGAAAAGA AGATAAACAT ACGTCACTAT 
GGAGACGGAA CTTACGGTGT TTCACTGGAT GAGACTGTCA TTGGTAGTGA TTTAATTGAC 
TTACTGTATG TGTTTGGAGC TAATGAAGAA GAGGCTGCCA GTGTCCTGTA CAGTGTATCT 
GATGCTGATT CTAATGTCAG TATTACTGGT AGTGGCCACG AGAGGGAAAC ACCTTATCTC 
ACTCATCCTG TCTTTAACAG TTATCATTCA GAGACTAAGT TGTTACGTTA CATGAAGGAG 
CTTGAGAACA GAGACCTCTC ACTCTGTCAC TCAATGATAC CACTGGGTTC CTGTACTATG 
AAACTCAACC CGACCAGTGC CCTACTCCCA GTCTCCCTAC CTCAATTCAA CACCATCCAT 
CCTTATGTAC CCAGTAACCA GACCACTGGA TACCAGAGTC TCATTGATGA GTTGGAGTCT 
CATTTGTGTA GTATCACTGG GTATGATAAG TTCTCCTTCC AGCCCAACAG TGGGGCCCAG 
GGGGAATACG CTGGACTCTG TGCTATACTG GCCTATCTGA GGGATAAAGG GGAGGGGCAG 
AGAGATATAT GTCTGATACC AGCCTCAGCT CATGGTACTA ACCCAGCCAG TGCAATAATG 
GCTGGTCTTA AAGTGGTTGA GATACCAATG AACAAAGAAG GAGAAGTCAC TAAAGACTCT 
TTTAAACAAA AAATAGAAGA AGCTGGTGAT AAGTTAGCCA CCATTATGGT GACCTATCCC 
TCCACTAGTG GTGTGTTTGA AGACTCCATT AGAGAGGTCT GTGACATGGT ACACTATTAT 
GGAGGACAGA AATGTAGTGT GATTTACATA CCACGCCACT TGTTACATCT TTGTGGTGTG 
TTTGAAGACT CCATTAGAGA GGTCTGTGAC ATGGTACACT ATTATGGAGG ACAGGTATAT 
GTAGATGGTG CTAATCTCAA TGCTCAGGTT GGGCTCTGTC GTCCTGGGGA TTATGGAGGA 
GATGTGTCTC ATCTCAACCT TCACAAGACC TTCTGTATAC CTCATGGTGG GGGCGGACCG 
GGAATGGGAC CTATTGGAGT TAAGGCTCAT TTAGCTCCAT ACCTACCTGG TCATCCAGTA 
ACTGGTCCTA ATCCATTAAA GTCCTTTGGA ACAGTATCAT CAGCAGCATG GGGCTCTGCC 
TCCATCATAC CAGTCTCATG GGCTTACATA CTAATGATGG GAAGCAAAGG ATTGAAGAAA 
GCTTCTGAAA TAGCAATAGT CAATGCTAAT TACATGGCAG CAAGACTAAA GGGACACTAT 
AATATCCTGT TCCGTGGAAC TAATGGATTC AATGCTCACG AGTTCATACT GGATACCAGG 
TGCTTTAAGA ACACTAGTGG CATTGAAGCT ATTGACATTG CTAAGAGAAT GCAGGACTAT 
GGGTTCCATG CCCCTACTGT CTCCTGGCCA GTCAGTAATA CTCTGATGAT TGAACCAACA 
GAGAGTGAAG GAAAGGCAGA ACTGGATCGA TACTGTGACG CACTAATATC TATTAGACAG 
GAAATCTCTG AAGTAGAAGA AGGCAAATAC GACATTAAGA ACAATGTACT AAAGAATGCT 
CCTCATCCCA TGTACTTGGT GACAGCCTCA AACTGGCCAT TCCCCTACTC AAGAGAGAAG 
GCTGCCTTTC CAGCTGAGTG GCTAAACCCA TACAATAAAG TCTGGACTGG CAGTGCAAGA 
GCTAATGATG TCCACGGTGA CAGGAACTTA GTTGCTACGC ACAACCAAGA GCTAAAACCT 
CTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019851639.1
CDS	70..2838
Translation	MNKVYRSYLGQGYYGCIVPPVIKRNVIENPGWITQYTPYQAELAQGRLESLMNYQTMIVDLTGLDIANASLLDEGTAAAEAVTLSYRMFRGKRNKYFIDEGIHSNSLAVIKTRAKFLNIDIVTGSYQDFVLTDDVCGVMLQYPNNDGIINNYHDIIQQAKGLKIPVAMATDLLALTILKSPGELGASIAFGNSQRFGVPMGCGGPHAAFFAVKDELKKQMPGRLVGKSWDQYGVPTYRLALQTREQFIRREKATSNICTAQALLANVSAMYAVYHGPKGLKDIAMEVHKATVLLAEGLRLLGWTINNETVFDTIMISNGDISTDEIQRRCDEKKINIRHYGDGTYGVSLDETVIGSDLIDLLYVFGANEEEAASVLYSVSDADSNVSITGSGHERETPYLTHPVFNSYHSETKLLRYMKELENRDLSLCHSMIPLGSCTMKLNPTSALLPVSLPQFNTIHPYVPSNQTTGYQSLIDELESHLCSITGYDKFSFQPNSGAQGEYAGLCAILAYLRDKGEGQRDICLIPASAHGTNPASAIMAGLKVVEIPMNKEGEVTKDSFKQKIEEAGDKLATIMVTYPSTSGVFEDSIREVCDMVHYYGGQKCSVIYIPRHLLHLCGVFEDSIREVCDMVHYYGGQVYVDGANLNAQVGLCRPGDYGGDVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPYLPGHPVTGPNPLKSFGTVSSAAWGSASIIPVSWAYILMMGSKGLKKASEIAIVNANYMAARLKGHYNILFRGTNGFNAHEFILDTRCFKNTSGIEAIDIAKRMQDYGFHAPTVSWPVSNTLMIEPTESEGKAELDRYCDALISIRQEISEVEEGKYDIKNNVLKNAPHPMYLVTASNWPFPYSREKAAFPAEWLNPYNKVWTGSARANDVHGDRNLVATHNQELKPLR

Target ORF information:

RefSeq Version	XM_019996080.1
Organism	Amphimedon queenslandica
Definition	Amphimedon queenslandica glycine dehydrogenase (decarboxylating), mitochondrial-like (LOC100633849), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019996080.1

ORF Insert Sequence:

ATGAACAAGG TGTATCGGTC TTATCTTGGT CAGGGGTACT ACGGATGTAT AGTACCTCCT 
GTTATCAAAA GAAACGTAAT AGAGAACCCA GGCTGGATAA CACAGTATAC TCCCTATCAA 
GCCGAGCTGG CTCAGGGTAG ACTGGAATCT CTCATGAACT ACCAGACAAT GATAGTCGAC 
CTAACTGGAC TGGATATTGC TAATGCTTCT CTTCTTGACG AAGGAACTGC AGCAGCTGAA 
GCTGTGACAC TCTCTTATAG AATGTTTAGA GGAAAAAGAA ACAAGTACTT CATTGATGAG 
GGGATCCACT CAAACTCGCT TGCGGTCATC AAAACGAGAG CAAAGTTCTT AAATATCGAC 
ATAGTGACTG GTTCGTATCA GGACTTTGTT TTAACTGATG ATGTGTGTGG AGTGATGCTC 
CAGTATCCTA ATAATGATGG TATCATTAAT AATTATCATG ACATCATACA GCAAGCTAAA 
GGATTAAAGA TCCCGGTTGC AATGGCTACT GATCTCCTAG CCCTCACTAT CCTCAAGTCT 
CCTGGTGAAT TAGGTGCCAG TATTGCCTTT GGTAACAGTC AGAGGTTCGG GGTACCAATG 
GGTTGTGGTG GTCCTCATGC TGCATTCTTT GCTGTTAAAG ACGAACTAAA GAAACAAATG 
CCTGGAAGAC TAGTGGGGAA GAGCTGGGAT CAGTATGGTG TCCCTACCTA CAGACTAGCA 
CTACAGACAA GAGAACAGTT CATAAGGAGA GAGAAGGCCA CCAGTAACAT ATGCACTGCC 
CAAGCTTTGC TGGCTAATGT ATCAGCTATG TATGCAGTGT ATCATGGACC CAAAGGACTG 
AAGGATATTG CAATGGAAGT ACATAAAGCA ACTGTACTAT TAGCTGAAGG CCTGAGACTG 
TTAGGTTGGA CTATAAATAA TGAAACTGTC TTTGATACAA TAATGATATC AAATGGAGAC 
ATTAGCACTG ATGAGATACA GAGGAGGTGT GACGAAAAGA AGATAAACAT ACGTCACTAT 
GGAGACGGAA CTTACGGTGT TTCACTGGAT GAGACTGTCA TTGGTAGTGA TTTAATTGAC 
TTACTGTATG TGTTTGGAGC TAATGAAGAA GAGGCTGCCA GTGTCCTGTA CAGTGTATCT 
GATGCTGATT CTAATGTCAG TATTACTGGT AGTGGCCACG AGAGGGAAAC ACCTTATCTC 
ACTCATCCTG TCTTTAACAG TTATCATTCA GAGACTAAGT TGTTACGTTA CATGAAGGAG 
CTTGAGAACA GAGACCTCTC ACTCTGTCAC TCAATGATAC CACTGGGTTC CTGTACTATG 
AAACTCAACC CGACCAGTGC CCTACTCCCA GTCTCCCTAC CTCAATTCAA CACCATCCAT 
CCTTATGTAC CCAGTAACCA GACCACTGGA TACCAGAGTC TCATTGATGA GTTGGAGTCT 
CATTTGTGTA GTATCACTGG GTATGATAAG TTCTCCTTCC AGCCCAACAG TGGGGCCCAG 
GGGGAATACG CTGGACTCTG TGCTATACTG GCCTATCTGA GGGATAAAGG GGAGGGGCAG 
AGAGATATAT GTCTGATACC AGCCTCAGCT CATGGTACTA ACCCAGCCAG TGCAATAATG 
GCTGGTCTTA AAGTGGTTGA GATACCAATG AACAAAGAAG GAGAAGTCAC TAAAGACTCT 
TTTAAACAAA AAATAGAAGA AGCTGGTGAT AAGTTAGCCA CCATTATGGT GACCTATCCC 
TCCACTAGTG GTGTGTTTGA AGACTCCATT AGAGAGGTCT GTGACATGGT ACACTATTAT 
GGAGGACAGA AATGTAGTGT GATTTACATA CCACGCCACT TGTTACATCT TTGTGGTGTG 
TTTGAAGACT CCATTAGAGA GGTCTGTGAC ATGGTACACT ATTATGGAGG ACAGGTATAT 
GTAGATGGTG CTAATCTCAA TGCTCAGGTT GGGCTCTGTC GTCCTGGGGA TTATGGAGGA 
GATGTGTCTC ATCTCAACCT TCACAAGACC TTCTGTATAC CTCATGGTGG GGGCGGACCG 
GGAATGGGAC CTATTGGAGT TAAGGCTCAT TTAGCTCCAT ACCTACCTGG TCATCCAGTA 
ACTGGTCCTA ATCCATTAAA GTCCTTTGGA ACAGTATCAT CAGCAGCATG GGGCTCTGCC 
TCCATCATAC CAGTCTCATG GGCTTACATA CTAATGATGG GAAGCAAAGG ATTGAAGAAA 
GCTTCTGAAA TAGCAATAGT CAATGCTAAT TACATGGCAG CAAGACTAAA GGGACACTAT 
AATATCCTGT TCCGTGGAAC TAATGGATTC AATGCTCACG AGTTCATACT GGATACCAGG 
TGCTTTAAGA ACACTAGTGG CATTGAAGCT ATTGACATTG CTAAGAGAAT GCAGGACTAT 
GGGTTCCATG CCCCTACTGT CTCCTGGCCA GTCAGTAATA CTCTGATGAT TGAACCAACA 
GAGAGTGAAG GAAAGGCAGA ACTGGATCGA TACTGTGACG CACTAATATC TATTAGACAG 
GAAATCTCTG AAGTAGAAGA AGGCAAATAC GACATTAAGA ACAATGTACT AAAGAATGCT 
CCTCATCCCA TGTACTTGGT GACAGCCTCA AACTGGCCAT TCCCCTACTC AAGAGAGAAG 
GCTGCCTTTC CAGCTGAGTG GCTAAACCCA TACAATAAAG TCTGGACTGG CAGTGCAAGA 
GCTAATGATG TCCACGGTGA CAGGAACTTA GTTGCTACGC ACAACCAAGA GCTAAAACCT 
CTTCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100633849 ( XM_019996081.1 ) cDNA ORF clone, Amphimedon queenslandica -> XP_019851640.1 Amphimedon queenslandica glycine dehydrogenase (decarboxylating), mitochondrial-like (LOC100633849), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OAm17178
Clone ID Related Accession (Same CDS sequence)	XM_019996081.1
Accession Version	XM_019996081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-01-17
Organism	Amphimedon queenslandica
Product	glycine dehydrogenase (decarboxylating), mitochondrial-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003546341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Amphimedon queenslandica Annotation Release 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAAGG TGTATCGGTC TTATCTTGGT CAGGGGTACT ACGGATGTAT AGTACCTCCT 
GTTATCAAAA GAAACGTAAT AGAGAACCCA GGCTGGATAA CACAGTATAC TCCCTATCAA 
GCCGAGCTGG CTCAGGGTAG ACTGGAATCT CTCATGAACT ACCAGACAAT GATAGTCGAC 
CTAACTGGAC TGGATATTGC TAATGCTTCT CTTCTTGACG AAGGAACTGC AGCAGCTGAA 
GCTGTGACAC TCTCTTATAG AATGTTTAGA GGAAAAAGAA ACAAGTACTT CATTGATGAG 
GGGATCCACT CAAACTCGCT TGCGGTCATC AAAACGAGAG CAAAGTTCTT AAATATCGAC 
ATAGTGACTG GTTCGTATCA GGACTTTGTT TTAACTGATG ATGTGTGTGG AGTGATGCTC 
CAGTATCCTA ATAATGATGG TATCATTAAT AATTATCATG ACATCATACA GCAAGCTAAA 
GGATTAAAGA TCCCGGTTGC AATGGCTACT GATCTCCTAG CCCTCACTAT CCTCAAGTCT 
CCTGGTGAAT TAGGTGCCAG TATTGCCTTT GGTAACAGTC AGAGGTTCGG GGTACCAATG 
GGTTGTGGTG GTCCTCATGC TGCATTCTTT GCTGTTAAAG ACGAACTAAA GAAACAAATG 
CCTGGAAGAC TAGTGGGGAA GAGCTGGGAT CAGTATGGTG TCCCTACCTA CAGACTAGCA 
CTACAGACAA GAGAACAGTT CATAAGGAGA GAGAAGGCCA CCAGTAACAT ATGCACTGCC 
CAAGCTTTGC TGGCTAATGT ATCAGCTATG TATGCAGTGT ATCATGGACC CAAAGGACTG 
AAGGATATTG CAATGGAAGT ACATAAAGCA ACTGTACTAT TAGCTGAAGG CCTGAGACTG 
TTAGGTTGGA CTATAAATAA TGAAACTGTC TTTGATACAA TAATGATATC AAATGGAGAC 
ATTAGCACTG ATGAGATACA GAGGAGGTGT GACGAAAAGA AGATAAACAT ACGTCACTAT 
GGAGACGGAA CTTACGGTGT TTCACTGGAT GAGACTGTCA TTGGTAGTGA TTTAATTGAC 
TTACTGTATG TGTTTGGAGC TAATGAAGAA GAGGCTGCCA GTGTCCTGTA CAGTGTATCT 
GATGCTGATT CTAATGTCAG TATTACTGGT AGTGGCCACG AGAGGGAAAC ACCTTATCTC 
ACTCATCCTG TCTTTAACAG TTATCATTCA GAGACTAAGT TGTTACGTTA CATGAAGGAG 
CTTGAGAACA GAGACCTCTC ACTCTGTCAC TCAATGATAC CACTGGGTTC CTGTACTATG 
AAACTCAACC CGACCAGTGC CCTACTCCCA GTCTCCCTAC CTCAATTCAA CACCATCCAT 
CCTTATGTAC CCAGTAACCA GACCACTGGA TACCAGAGTC TCATTGATGA GTTGGAGTCT 
CATTTGTGTA GTATCACTGG GTATGATAAG TTCTCCTTCC AGCCCAACAG TGGGGCCCAG 
GGGGAATACG CTGGACTCTG TGCTATACTG GCCTATCTGA GGGATAAAGG GGAGGGGCAG 
AGAGATATAT GTCTGATACC AGCCTCAGCT CATGGTACTA ACCCAGCCAG TGCAATAATG 
GCTGGTCTTA AAGTGGTTGA GATACCAATG AACAAAGAAG GAGAAGTCAC TAAAGACTCT 
TTTAAACAAA AAATAGAAGA AGCTGGTGAT AAGTTAGCCA CCATTATGGT GACCTATCCC 
TCCACTAGTG GTGTGTTTGA AGACTCCATT AGAGAGGTCT GTGACATGGT ACACTATTAT 
GGAGGACAGG TATATGTAGA TGGTGCTAAT CTCAATGCTC AGGTTGGGCT CTGTCGTCCT 
GGGGATTATG GAGGAGATGT GTCTCATCTC AACCTTCACA AGACCTTCTG TATACCTCAT 
GGTGGGGGCG GACCGGGAAT GGGACCTATT GGAGTTAAGG CTCATTTAGC TCCATACCTA 
CCTGGTCATC CAGTAACTGG TCCTAATCCA TTAAAGTCCT TTGGAACAGT ATCATCAGCA 
GCATGGGGCT CTGCCTCCAT CATACCAGTC TCATGGGCTT ACATACTAAT GATGGGAAGC 
AAAGGATTGA AGAAAGCTTC TGAAATAGCA ATAGTCAATG CTAATTACAT GGCAGCAAGA 
CTAAAGGGAC ACTATAATAT CCTGTTCCGT GGAACTAATG GATTCAATGC TCACGAGTTC 
ATACTGGATA CCAGGTGCTT TAAGAACACT AGTGGCATTG AAGCTATTGA CATTGCTAAG 
AGAATGCAGG ACTATGGGTT CCATGCCCCT ACTGTCTCCT GGCCAGTCAG TAATACTCTG 
ATGATTGAAC CAACAGAGAG TGAAGGAAAG GCAGAACTGG ATCGATACTG TGACGCACTA 
ATATCTATTA GACAGGAAAT CTCTGAAGTA GAAGAAGGCA AATACGACAT TAAGAACAAT 
GTACTAAAGA ATGCTCCTCA TCCCATGTAC TTGGTGACAG CCTCAAACTG GCCATTCCCC 
TACTCAAGAG AGAAGGCTGC CTTTCCAGCT GAGTGGCTAA ACCCATACAA TAAAGTCTGG 
ACTGGCAGTG CAAGAGCTAA TGATGTCCAC GGTGACAGGA ACTTAGTTGC TACGCACAAC 
CAAGAGCTAA AACCTCTTCG GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019851640.1
CDS	70..2733
Translation	MNKVYRSYLGQGYYGCIVPPVIKRNVIENPGWITQYTPYQAELAQGRLESLMNYQTMIVDLTGLDIANASLLDEGTAAAEAVTLSYRMFRGKRNKYFIDEGIHSNSLAVIKTRAKFLNIDIVTGSYQDFVLTDDVCGVMLQYPNNDGIINNYHDIIQQAKGLKIPVAMATDLLALTILKSPGELGASIAFGNSQRFGVPMGCGGPHAAFFAVKDELKKQMPGRLVGKSWDQYGVPTYRLALQTREQFIRREKATSNICTAQALLANVSAMYAVYHGPKGLKDIAMEVHKATVLLAEGLRLLGWTINNETVFDTIMISNGDISTDEIQRRCDEKKINIRHYGDGTYGVSLDETVIGSDLIDLLYVFGANEEEAASVLYSVSDADSNVSITGSGHERETPYLTHPVFNSYHSETKLLRYMKELENRDLSLCHSMIPLGSCTMKLNPTSALLPVSLPQFNTIHPYVPSNQTTGYQSLIDELESHLCSITGYDKFSFQPNSGAQGEYAGLCAILAYLRDKGEGQRDICLIPASAHGTNPASAIMAGLKVVEIPMNKEGEVTKDSFKQKIEEAGDKLATIMVTYPSTSGVFEDSIREVCDMVHYYGGQVYVDGANLNAQVGLCRPGDYGGDVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPYLPGHPVTGPNPLKSFGTVSSAAWGSASIIPVSWAYILMMGSKGLKKASEIAIVNANYMAARLKGHYNILFRGTNGFNAHEFILDTRCFKNTSGIEAIDIAKRMQDYGFHAPTVSWPVSNTLMIEPTESEGKAELDRYCDALISIRQEISEVEEGKYDIKNNVLKNAPHPMYLVTASNWPFPYSREKAAFPAEWLNPYNKVWTGSARANDVHGDRNLVATHNQELKPLR

Target ORF information:

RefSeq Version	XM_019996081.1
Organism	Amphimedon queenslandica
Definition	Amphimedon queenslandica glycine dehydrogenase (decarboxylating), mitochondrial-like (LOC100633849), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019996081.1

ORF Insert Sequence:

ATGAACAAGG TGTATCGGTC TTATCTTGGT CAGGGGTACT ACGGATGTAT AGTACCTCCT 
GTTATCAAAA GAAACGTAAT AGAGAACCCA GGCTGGATAA CACAGTATAC TCCCTATCAA 
GCCGAGCTGG CTCAGGGTAG ACTGGAATCT CTCATGAACT ACCAGACAAT GATAGTCGAC 
CTAACTGGAC TGGATATTGC TAATGCTTCT CTTCTTGACG AAGGAACTGC AGCAGCTGAA 
GCTGTGACAC TCTCTTATAG AATGTTTAGA GGAAAAAGAA ACAAGTACTT CATTGATGAG 
GGGATCCACT CAAACTCGCT TGCGGTCATC AAAACGAGAG CAAAGTTCTT AAATATCGAC 
ATAGTGACTG GTTCGTATCA GGACTTTGTT TTAACTGATG ATGTGTGTGG AGTGATGCTC 
CAGTATCCTA ATAATGATGG TATCATTAAT AATTATCATG ACATCATACA GCAAGCTAAA 
GGATTAAAGA TCCCGGTTGC AATGGCTACT GATCTCCTAG CCCTCACTAT CCTCAAGTCT 
CCTGGTGAAT TAGGTGCCAG TATTGCCTTT GGTAACAGTC AGAGGTTCGG GGTACCAATG 
GGTTGTGGTG GTCCTCATGC TGCATTCTTT GCTGTTAAAG ACGAACTAAA GAAACAAATG 
CCTGGAAGAC TAGTGGGGAA GAGCTGGGAT CAGTATGGTG TCCCTACCTA CAGACTAGCA 
CTACAGACAA GAGAACAGTT CATAAGGAGA GAGAAGGCCA CCAGTAACAT ATGCACTGCC 
CAAGCTTTGC TGGCTAATGT ATCAGCTATG TATGCAGTGT ATCATGGACC CAAAGGACTG 
AAGGATATTG CAATGGAAGT ACATAAAGCA ACTGTACTAT TAGCTGAAGG CCTGAGACTG 
TTAGGTTGGA CTATAAATAA TGAAACTGTC TTTGATACAA TAATGATATC AAATGGAGAC 
ATTAGCACTG ATGAGATACA GAGGAGGTGT GACGAAAAGA AGATAAACAT ACGTCACTAT 
GGAGACGGAA CTTACGGTGT TTCACTGGAT GAGACTGTCA TTGGTAGTGA TTTAATTGAC 
TTACTGTATG TGTTTGGAGC TAATGAAGAA GAGGCTGCCA GTGTCCTGTA CAGTGTATCT 
GATGCTGATT CTAATGTCAG TATTACTGGT AGTGGCCACG AGAGGGAAAC ACCTTATCTC 
ACTCATCCTG TCTTTAACAG TTATCATTCA GAGACTAAGT TGTTACGTTA CATGAAGGAG 
CTTGAGAACA GAGACCTCTC ACTCTGTCAC TCAATGATAC CACTGGGTTC CTGTACTATG 
AAACTCAACC CGACCAGTGC CCTACTCCCA GTCTCCCTAC CTCAATTCAA CACCATCCAT 
CCTTATGTAC CCAGTAACCA GACCACTGGA TACCAGAGTC TCATTGATGA GTTGGAGTCT 
CATTTGTGTA GTATCACTGG GTATGATAAG TTCTCCTTCC AGCCCAACAG TGGGGCCCAG 
GGGGAATACG CTGGACTCTG TGCTATACTG GCCTATCTGA GGGATAAAGG GGAGGGGCAG 
AGAGATATAT GTCTGATACC AGCCTCAGCT CATGGTACTA ACCCAGCCAG TGCAATAATG 
GCTGGTCTTA AAGTGGTTGA GATACCAATG AACAAAGAAG GAGAAGTCAC TAAAGACTCT 
TTTAAACAAA AAATAGAAGA AGCTGGTGAT AAGTTAGCCA CCATTATGGT GACCTATCCC 
TCCACTAGTG GTGTGTTTGA AGACTCCATT AGAGAGGTCT GTGACATGGT ACACTATTAT 
GGAGGACAGG TATATGTAGA TGGTGCTAAT CTCAATGCTC AGGTTGGGCT CTGTCGTCCT 
GGGGATTATG GAGGAGATGT GTCTCATCTC AACCTTCACA AGACCTTCTG TATACCTCAT 
GGTGGGGGCG GACCGGGAAT GGGACCTATT GGAGTTAAGG CTCATTTAGC TCCATACCTA 
CCTGGTCATC CAGTAACTGG TCCTAATCCA TTAAAGTCCT TTGGAACAGT ATCATCAGCA 
GCATGGGGCT CTGCCTCCAT CATACCAGTC TCATGGGCTT ACATACTAAT GATGGGAAGC 
AAAGGATTGA AGAAAGCTTC TGAAATAGCA ATAGTCAATG CTAATTACAT GGCAGCAAGA 
CTAAAGGGAC ACTATAATAT CCTGTTCCGT GGAACTAATG GATTCAATGC TCACGAGTTC 
ATACTGGATA CCAGGTGCTT TAAGAACACT AGTGGCATTG AAGCTATTGA CATTGCTAAG 
AGAATGCAGG ACTATGGGTT CCATGCCCCT ACTGTCTCCT GGCCAGTCAG TAATACTCTG 
ATGATTGAAC CAACAGAGAG TGAAGGAAAG GCAGAACTGG ATCGATACTG TGACGCACTA 
ATATCTATTA GACAGGAAAT CTCTGAAGTA GAAGAAGGCA AATACGACAT TAAGAACAAT 
GTACTAAAGA ATGCTCCTCA TCCCATGTAC TTGGTGACAG CCTCAAACTG GCCATTCCCC 
TACTCAAGAG AGAAGGCTGC CTTTCCAGCT GAGTGGCTAA ACCCATACAA TAAAGTCTGG 
ACTGGCAGTG CAAGAGCTAA TGATGTCCAC GGTGACAGGA ACTTAGTTGC TACGCACAAC 
CAAGAGCTAA AACCTCTTCG GTAA